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Enum: AsoMechanismEnum

Molecular mechanism of action of an antisense oligonucleotide, following the three core ASO paradigms (RNase H-mediated degradation, splice modulation, and steric blockade) described in Sang et al. 2024 (PMID:38914784).

URI: dismech:enum/AsoMechanismEnum

Permissible Values

Value Meaning Description Additional Info
RNASE_H_KNOCKDOWN GO:0004523 ASO:RNA heteroduplex recruits RNase H1 to cleave the target mRNA, reducing a ... Title: RNase H knockdown
SPLICE_MODULATION_EXON_SKIPPING None ASO occludes a splice site or splicing element to exclude an exon, restoring ... Title: Splice modulation (exon skipping)
SPLICE_MODULATION_EXON_INCLUSION None ASO blocks an intronic splicing silencer to promote exon inclusion (e Title: Splice modulation (exon inclusion)
STERIC_BLOCKADE None ASO sterically blocks ribosome access or other RNA-protein interactions witho... Title: Steric translational blockade
MIRNA_MODULATION None ASO sequesters or inhibits a microRNA (antimiR) or blocks a miRNA binding sit... Title: miRNA modulation

Slots

Name Description
aso_mechanism Molecular mechanism of action of an antisense oligonucleotide

Identifier and Mapping Information

Schema Source

  • from schema: https://w3id.org/monarch-initiative/dismech

LinkML Source

name: AsoMechanismEnum
description: Molecular mechanism of action of an antisense oligonucleotide, following
  the three core ASO paradigms (RNase H-mediated degradation, splice modulation, and
  steric blockade) described in Sang et al. 2024 (PMID:38914784).
from_schema: https://w3id.org/monarch-initiative/dismech
rank: 1000
permissible_values:
  RNASE_H_KNOCKDOWN:
    text: RNASE_H_KNOCKDOWN
    description: ASO:RNA heteroduplex recruits RNase H1 to cleave the target mRNA,
      reducing a toxic or gain-of-function protein
    meaning: GO:0004523
    title: RNase H knockdown
    aliases:
    - RNA-DNA hybrid ribonuclease activity
  SPLICE_MODULATION_EXON_SKIPPING:
    text: SPLICE_MODULATION_EXON_SKIPPING
    description: ASO occludes a splice site or splicing element to exclude an exon,
      restoring an in-frame transcript (e.g., DMD exon skipping)
    title: Splice modulation (exon skipping)
  SPLICE_MODULATION_EXON_INCLUSION:
    text: SPLICE_MODULATION_EXON_INCLUSION
    description: ASO blocks an intronic splicing silencer to promote exon inclusion
      (e.g., nusinersen at SMN2 ISS-N1)
    title: Splice modulation (exon inclusion)
  STERIC_BLOCKADE:
    text: STERIC_BLOCKADE
    description: ASO sterically blocks ribosome access or other RNA-protein interactions
      without inducing cleavage (e.g., fomivirsen)
    title: Steric translational blockade
  MIRNA_MODULATION:
    text: MIRNA_MODULATION
    description: ASO sequesters or inhibits a microRNA (antimiR) or blocks a miRNA
      binding site
    title: miRNA modulation