Pathophysiology Nodes

6
6 shared nodes are defined in this module.

Cell Types

3
Hepatocyte CL:0000182 Cell Ontology (CL) Relation: this mechanism module involves this cell type This mechanism module involves Hepatocyte (CL:0000182). CL:0000182 is a cell type from the Cell Ontology. B cell CL:0000236 Cell Ontology (CL) Relation: this mechanism module involves this cell type This mechanism module involves B cell (CL:0000236). CL:0000236 is a cell type from the Cell Ontology. Plasma cell CL:0000786 Cell Ontology (CL) Relation: this mechanism module involves this cell type This mechanism module involves Plasma cell (CL:0000786). CL:0000786 is a cell type from the Cell Ontology.

Biological Processes

8
Interleukin-6 production GO:0032635 Gene Ontology (GO) Relation: this mechanism module involves this biological process This mechanism module involves increased Interleukin-6 production (GO:0032635). GO:0032635 is a biological process from the Gene Ontology. INCREASED Interleukin-6-mediated signaling pathway GO:0070102 Gene Ontology (GO) Relation: this mechanism module involves this biological process This mechanism module involves increased Interleukin-6-mediated signaling pathway (GO:0070102). GO:0070102 is a biological process from the Gene Ontology. INCREASED JAK-STAT signaling GO:0007259 Gene Ontology (GO) Relation: this mechanism module involves this biological process This mechanism module involves increased JAK-STAT signaling (GO:0007259). GO:0007259 is a biological process from the Gene Ontology. INCREASED MAPK cascade GO:0000165 Gene Ontology (GO) Relation: this mechanism module involves this biological process This mechanism module involves increased MAPK cascade (GO:0000165). GO:0000165 is a biological process from the Gene Ontology. INCREASED Acute-phase response GO:0006953 Gene Ontology (GO) Relation: this mechanism module involves this biological process This mechanism module involves increased Acute-phase response (GO:0006953). GO:0006953 is a biological process from the Gene Ontology. INCREASED Plasma cell differentiation GO:0002317 Gene Ontology (GO) Relation: this mechanism module involves this biological process This mechanism module involves increased Plasma cell differentiation (GO:0002317). GO:0002317 is a biological process from the Gene Ontology. INCREASED Immunoglobulin production GO:0002377 Gene Ontology (GO) Relation: this mechanism module involves this biological process This mechanism module involves increased Immunoglobulin production (GO:0002377). GO:0002377 is a biological process from the Gene Ontology. INCREASED Inflammatory response GO:0006954 Gene Ontology (GO) Relation: this mechanism module involves this biological process This mechanism module involves increased Inflammatory response (GO:0006954). GO:0006954 is a biological process from the Gene Ontology. INCREASED
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Notes

Conforming entries substitute the disorder-specific IL-6 source at the trigger node and keep the rest of the chain: lymph node stromal and plasma cells in idiopathic multicentric Castleman disease, lytically infected plasmablasts producing a viral IL-6 homolog in HHV-8-associated multicentric Castleman disease, synovial fibroblasts and macrophages in rheumatoid arthritis, activated macrophages in Still disease and in cytokine release syndrome, and tumour or stromal cells in IL-6-driven malignancy. The clinical read-out set at the consequence node is likewise substituted. Relationship to `cytokine_storm_hyperinflammation`, which also has IL-6 at its centre: that module models the *generation* of an acute, life-threatening storm - an overwhelming immune trigger driving a self-amplifying myeloid cytokine loop, ending in endothelial capillary leak, shock and multiorgan dysfunction. This module picks up at the cytokine and models how the IL-6 *signal is transduced* and which tissue programs it turns on: gp130 engagement, JAK-STAT3, hepatic acute-phase reprogramming and B-lineage differentiation. The two therefore overlap at the IL-6 node and diverge on either side of it. Use the storm module for a disorder whose defining course is acute hyperinflammation ending in shock; use this one for a disorder whose IL-6 output drives chronic acute-phase and plasma-cell programs, which is the case for the indolent Castleman subtypes where shock never occurs. A single node conforms to one or the other, since `conforms_to` is single-valued; a disorder that does both - iMCD-TAFRO - conforms its capillary-leak node to the storm module and its cytokine chain here. This module is deliberately distinct from `jak_stat_pathway_activation`, and a node should not conform to both. That module covers cell-intrinsic, constitutive, ligand-INDEPENDENT activation from an activating lesion in a receptor, JAK or STAT - or from lost SOCS feedback - and terminates in cytokine-independent proliferation. This module covers the opposite case: a structurally normal pathway driven by an excess of ligand, where removing the ligand or blocking its receptor switches the pathway off. A disorder in which IL-6 blockade produces clinical remission conforms here, not there. This is not an Xogenesis module: the terminal output is a systemic physiological state, not a pathological material anatomical entity, so no MPATH/OGMS anchor stanza applies.

Used By Disorder Entries

2

Pathograph

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Pathograph: causal mechanism network for Interleukin-6 Hypercytokinemia Module Interactive directed graph showing how this shared module's pathophysiology nodes connect.

Pathophysiology

6
Sustained Interleukin-6 Oversupply
trigger
IL-6 is produced in excess of what its clearance and negative-feedback machinery can absorb, and the excess is sustained rather than transient. The producing cell is the disorder-specific substitution and varies widely - stromal cells, plasma cells, macrophages, endothelium, synovial fibroblasts, tumour cells - and in one case the ligand is not host IL-6 at all but a virally encoded homolog. Normal control operates through induction of suppressor molecules after pathway activation and through circulating soluble IL-6 receptor and gp130; sustained oversupply overwhelms it.
Interleukin-6 production GO:0032635 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves increased Interleukin-6 production (GO:0032635). GO:0032635 is a biological process from the Gene Ontology. INCREASED
gp130 Receptor Complex Engagement
amplifier
IL-6 signals through a complex of IL-6, an IL-6 receptor and the signal-transducing subunit gp130. Three modes exist: classic signaling through the membrane-bound receptor, trans-signaling through a soluble receptor, and trans-presentation from a neighbouring cell. The amplification that makes hypercytokinemia systemic is trans-signaling: only a few cell types express the membrane receptor, whereas gp130 is ubiquitous, so a soluble-receptor complex dramatically enlarges the spectrum of responder cells. Pro-inflammatory responses are attributed rather to trans-signaling and regenerative or anti-inflammatory responses to classic signaling.
Interleukin-6-mediated signaling pathway GO:0070102 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves increased Interleukin-6-mediated signaling pathway (GO:0070102). GO:0070102 is a biological process from the Gene Ontology. INCREASED
JAK-STAT3 Activation in Responder Cells
central effector
The rate-limiting, disorder-agnostic step and the key conformance target. gp130 dimerization activates Janus kinases, which phosphorylate tyrosine residues in the gp130 cytoplasmic tail. Two arms follow: recruitment and phosphorylation of STAT transcription factors, which dimerize, translocate to the nucleus and activate target genes; and engagement of SHP-2 with activation of the Ras/Raf/MAPK pathway. Every conforming disorder funnels through this node regardless of what supplies the ligand, and it is the point a JAK inhibitor acts on.
JAK-STAT signaling GO:0007259 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves increased JAK-STAT signaling, annotated with cell surface receptor signaling pathway via JAK-STAT (GO:0007259). GO:0007259 is a biological process from the Gene Ontology. INCREASED MAPK cascade GO:0000165 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves increased MAPK cascade (GO:0000165). GO:0000165 is a biological process from the Gene Ontology. INCREASED
Hepatic Acute-Phase Reprogramming
effector
STAT3-driven transcription in the hepatocyte induces the positive acute-phase proteins, including C-reactive protein and fibrinogen, while suppressing albumin synthesis; hepcidin induction restricts iron availability and produces the anemia of inflammation. These outputs reverse on IL-6 blockade, and their laboratory markers normalize in a reproducible sequence, which is the evidence that they are downstream of the cytokine rather than independent.
Hepatocyte CL:0000182 Cell Ontology (CL) Relation: this pathophysiological event involves this cell type This pathophysiological event involves Hepatocyte (CL:0000182). CL:0000182 is a cell type from the Cell Ontology.
Acute-phase response GO:0006953 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves increased Acute-phase response (GO:0006953). GO:0006953 is a biological process from the Gene Ontology. INCREASED
B-Lineage Differentiation and Immunoglobulin Output
effector
IL-6 drives IL-21 production by CD4+ T cells, and STAT3 in the B cell is then required for optimal immunoglobulin production and for upregulation of the master plasma cell factor PRDM1. The output is plasma cell differentiation and immunoglobulin secretion. This is a separate effector arm from the hepatic one: it acts on a different cell lineage through an IL-21 intermediate, and its clinical read-out (immunoglobulin) is the last to normalize on IL-6 blockade rather than among the first.
B cell CL:0000236 Cell Ontology (CL) Relation: this pathophysiological event involves this cell type This pathophysiological event involves B cell (CL:0000236). CL:0000236 is a cell type from the Cell Ontology. Plasma cell CL:0000786 Cell Ontology (CL) Relation: this pathophysiological event involves this cell type This pathophysiological event involves Plasma cell (CL:0000786). CL:0000786 is a cell type from the Cell Ontology.
Plasma cell differentiation GO:0002317 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves increased Plasma cell differentiation (GO:0002317). GO:0002317 is a biological process from the Gene Ontology. INCREASED Immunoglobulin production GO:0002377 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves increased Immunoglobulin production (GO:0002377). GO:0002377 is a biological process from the Gene Ontology. INCREASED
Systemic Inflammatory Syndrome
consequence
The clinical state: fever, fatigue and weight loss, anemia of inflammation, hypoalbuminemia with third-space fluid accumulation, a raised C-reactive protein and erythrocyte sedimentation rate, hypergammaglobulinemia, and platelet counts driven away from normal. Which of these dominate is the disorder-specific substitution. The defining property for conformance is that the syndrome remits when the IL-6 axis is blocked - if it does not, the disorder is being driven by something this module does not model.
Inflammatory response GO:0006954 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves increased Inflammatory response (GO:0006954). GO:0006954 is a biological process from the Gene Ontology. INCREASED
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Treatments

3
Anti-IL-6 Monoclonal Antibody
anti-IL-6 monoclonal antibody therapy NCIT:C15986 NCI Thesaurus (NCIT) Relation: this treatment is this clinical intervention This treatment is anti-IL-6 monoclonal antibody therapy, annotated with Pharmacotherapy (NCIT:C15986). NCIT:C15986 is a clinical intervention from the NCI Thesaurus.
Agent: siltuximab NCIT:C61084 NCI Thesaurus (NCIT) Relation: this treatment uses this therapeutic agent This treatment uses siltuximab (NCIT:C61084). NCIT:C61084 is a therapeutic agent from the NCI Thesaurus.
Platform: Monoclonal antibody
Neutralizes the cytokine itself before it can assemble a receptor complex. Siltuximab is the worked example and is approved for idiopathic multicentric Castleman disease.
Mechanism target:
INHIBITS Sustained Interleukin-6 Oversupply — Removes the ligand, so no receptor complex forms.
Evidence: 1
Anti-IL-6-Receptor Monoclonal Antibody
anti-IL-6 receptor monoclonal antibody therapy NCIT:C15986 NCI Thesaurus (NCIT) Relation: this treatment is this clinical intervention This treatment is anti-IL-6 receptor monoclonal antibody therapy, annotated with Pharmacotherapy (NCIT:C15986). NCIT:C15986 is a clinical intervention from the NCI Thesaurus.
Agent: tocilizumab NCIT:C84217 NCI Thesaurus (NCIT) Relation: this treatment uses this therapeutic agent This treatment uses tocilizumab (NCIT:C84217). NCIT:C84217 is a therapeutic agent from the NCI Thesaurus.
Platform: Monoclonal antibody
Blocks the receptor rather than the ligand. Tocilizumab is the worked example and is approved for rheumatoid arthritis, cytokine release syndrome and idiopathic multicentric Castleman disease - three disorders that conform to this module by different triggers.
Mechanism target:
INHIBITS gp130 Receptor Complex Engagement — Blocks both membrane and soluble IL-6 receptor, so neither classic nor trans-signaling can assemble a gp130 complex.
Evidence: 1
JAK Inhibition
Pharmacotherapy NCIT:C15986 NCI Thesaurus (NCIT) Relation: this treatment is this clinical intervention This treatment is Pharmacotherapy (NCIT:C15986). NCIT:C15986 is a clinical intervention from the NCI Thesaurus.
Agent: ruxolitinib CHEBI:66919 Chemical Entities of Biological Interest (CHEBI) Relation: this treatment uses this therapeutic agent This treatment uses ruxolitinib (CHEBI:66919). CHEBI:66919 is a therapeutic agent from Chemical Entities of Biological Interest.
Platform: Small molecule
Acts inside the responder cell at the central effector node, downstream of both the cytokine and its receptor. This is the rational option when the ligand is not host IL-6 or when more than one gp130-family cytokine is contributing, since it does not depend on neutralizing a single ligand.
Mechanism target:
INHIBITS JAK-STAT3 Activation in Responder Cells — Inhibits the Janus kinases downstream of gp130, the shared node for every gp130-family ligand.
Evidence: 1