X-linked Chondrodysplasia Punctata 2

Mendelian MONDO:0020603 Pathograph 23 Show in embeddings browser X-linked Chondrodysplasia Punctata Sterol Biosynthesis Disorder Hereditary Skin Disorder

X-linked dominant chondrodysplasia punctata type 2 (CDPX2, Conradi-Hunermann-Happle syndrome) is caused by heterozygous loss-of-function variants in EBP, which encodes the 3-beta-hydroxysteroid-delta8,delta7-isomerase (emopamil-binding protein) that isomerizes the double bond one step below lanosterol in post-squalene cholesterol biosynthesis. As in the other distal-cholesterol-pathway disorders the lesion is dual: cells lose the pathway end product and simultaneously accumulate the immediately upstream intermediates, 8(9)-cholestenol and 8-dehydrocholesterol, whose elevation in plasma, skin scale, or cultured cells is the biochemical signature of the disease. What makes CDPX2 mechanistically distinctive within its ISDS nosology group is not the enzymology but the fact that the phenotype is a *mosaic* readout of it. EBP is X-linked, so a heterozygous female is a functional mosaic of enzyme-competent and enzyme-deficient clones, and the disease is written onto the body in that clonal geometry - blaschkolinear ichthyotic scaling in the newborn that later resolves into linear follicular atrophoderma and scarring alopecia along the same lines, asymmetric rather than symmetric rhizomelic shortening, sectorial rather than whole-lens cataract, and side-to-side differences in epiphyseal stippling. The same mosaicism explains the disorder's unusual epidemiology at both extremes: at least 95% of live-born affected individuals are female because hemizygous males are usually lost in early gestation, while the affected males who do survive do so by being mosaic themselves (post-zygotic variants or a 47,XXY karyotype) or by carrying a hypomorphic allele that leaves residual isomerase activity. Because clone proportions vary between individuals and between body regions, the severity range is correspondingly enormous - from fetal demise with multiple malformations to obligate carrier mothers whose only finding is a strand of atrophic scalp skin, recognized only after a severely affected daughter is born.

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1
Mappings
1
Inheritance
9
Pathophys.
12
Phenotypes
2
Gaps
23
Pathograph
1
Genes
3
Medical Actions
3
Differentials
1
Models
5
References
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Classifications

Harrison's Part
GENETICS ENVIRONMENT DISEASE DERMATOLOGY ENDOCRINOLOGY METABOLISM
ICIMD (Inherited Metabolic Disorders)
sterol metabolism
ISDS Skeletal Nosology
chondrodysplasia punctata
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Mappings

MONDO
MONDO:0020603 X-linked chondrodysplasia punctata 2
skos:exactMatch MONDO
The dismech entry and the MONDO class denote the same entity: EBP-related X-linked dominant chondrodysplasia punctata. The entry additionally discusses the hypomorphic-allele male phenotype, which some authors argue is a distinct X-linked recessive entity; that is recorded as a discussion rather than folded into the mapping.
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Inheritance

1
X-linked dominant inheritance with male lethality HP:0001423
Inherited in an X-linked manner with early gestational lethality in hemizygous males. About 60% of affected females carry a de novo variant; the remainder inherit it from a mildly affected mother or from a somatically/gonadally mosaic father. A female who inherits a variant is a heterozygote whose severity cannot be predicted from the genotype.
X-linked dominant inheritance
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"EBP-CDPX is inherited in an X-linked manner with typical (but not absolute) early gestational male lethality."
States the inheritance pattern and the male-lethality qualifier.
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Discussions and Knowledge Gaps

2
Is the phenotype of a male hemizygous for a hypomorphic EBP allele a mild variant of CDPX2, or a separate X-linked recessive disorder that happens to share the gene?
KNOWLEDGE GAP OPEN hypomorphic_male_entity
Two distinct routes let a male with an EBP variant survive, and they predict different phenotypes. A mosaic male, or one with a null allele plus a supernumerary X, has enzyme-competent clones and presents like a female CDPX2 patient. A male hemizygous for a hypomorphic allele has no competent clones anywhere but retains partial activity in all of them, and the reported phenotype - digital abnormalities, intellectual disability, and short stature, without the ichthyosis and stippling of CDPX2 - does not look like CDPX2 at all. Whether that is a nosologically separate entity or the low-activity end of one continuum is unsettled, and it matters for this entry's boundaries: the ISDS nosology lists only one EBP row, and MONDO carries only MONDO:0020603, so a decision either way would change what this entry covers. dismech currently treats it as within scope and flags the question here rather than creating a second entry on one report.
Show evidence (1 reference)
PMID:24700572 SUPPORT Human Clinical
"The molecular and biochemical evidence convincingly supports the pathogenicity and association of the p.I75N mutation with this newly described phenotype."
The authors argue for a distinct male entity on the strength of one family with biochemical confirmation - enough to raise the question, not to settle it.
Which arm of the dual lesion - cholesterol starvation or accumulation of the upstream sterols - actually produces the punctate cartilage calcification, and by what route?
KNOWLEDGE GAP OPEN sterol_to_stippling_route
The enzymology of CDPX2 is settled and the sterol profile is diagnostic, but the step from either sterol abnormality to abnormal calcification of cartilage is not. The gene-discovery paper put it no more strongly than that the results "suggest a role for sterols in bone development", and that remains the state of the argument. The question is answerable in principle, because CHILD syndrome supplies a precedent: there, a topical trial separating cholesterol replacement from suppression of precursor flux showed that neither arm alone accounted for the skin phenotype. No equivalent dissection has been done for the CDPX2 skeleton, and the two mechanism edges in this entry are annotated as having unknown intermediates for that reason.
Show evidence (1 reference)
PMID:10391219 SUPPORT Human Clinical
"Our results indicate that defects in sterol-delta8-isomerase cause CDPX2 and suggest a role for sterols in bone development."
The authors' own hedged phrasing marks the gap between the established enzyme defect and the skeletal phenotype.

Pathophysiology

9
EBP Sterol-Delta8-Isomerase Deficiency
Loss-of-function variants in EBP abolish or reduce 3-beta-hydroxysteroid-delta8,delta7-isomerase activity, the step that moves the sterol double bond from position 8 to position 7 in the conversion of lanosterol to cholesterol. The enzymatic assignment was made in the reverse of the usual direction: the sterol profile of patients was characterized first and pointed at the isomerase, and the candidate gene was then found mutated in every proband tested.
Genetic context EBP hgnc:3133 HUGO Gene Nomenclature Committee (hgnc) Relation: this genetic context concerns this gene This genetic context concerns EBP (hgnc:3133). hgnc:3133 is a gene from the HUGO Gene Nomenclature Committee. variant_origin: GERMLINE zygosity: HETEROZYGOUS functional_impact_category: LOSS_OF_FUNCTION
Heterozygous germline (or, in the surviving affected male, post-zygotic mosaic) loss-of-function variants. Missense alleles were shown to be functionally deficient by complementation in an isomerase-deficient yeast strain. Zygosity is recorded as heterozygous because that is the state in the affected female who constitutes at least 95% of live births; a hemizygous male with a null allele is generally not viable.
cholesterol biosynthesis GO:0006695 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves decreased cholesterol biosynthesis, annotated with cholesterol biosynthetic process (GO:0006695). GO:0006695 is a biological process from the Gene Ontology. ↓ DECREASED
sterol delta8-delta7 isomerase activity GO:0000247 Gene Ontology (GO) Relation: this pathophysiological event involves this molecular function This pathophysiological event involves sterol delta8-delta7 isomerase activity, annotated with C-8 sterol isomerase activity (GO:0000247), qualified as loss of function. GO:0000247 is a molecular function from the Gene Ontology. ⇓ LOSS OF FUNCTION
Show evidence (2 references)
PMID:10391219 SUPPORT Human Clinical
"Using SSCP analysis and sequencing of genomic DNA, we found EBP mutations in all probands."
Establishes EBP variants as the cause in the biochemically defined patient cohort.
PMID:10391219 SUPPORT In Vitro
"We confirmed the functional significance of two missense alleles by expressing them in a sterol-delta8-isomerase-deficient yeast strain."
A complementation assay in an isomerase-deficient yeast strain establishes that the patient missense alleles are functionally deficient, not merely associated.
Accumulation of 8(9)-Cholestenol and 8-Dehydrocholesterol
The two sterols immediately upstream of the isomerase accumulate. Their elevation is both the diagnostic marker of the disease and, in the shared model for this disorder class, a potentially bioactive or toxic insult in its own right rather than an inert by-product. Because the accumulation occurs only in mutant-expressing clones, it is a cell-autonomous signal that tracks the mosaic.
Show evidence (1 reference)
PMID:10391219 SUPPORT Human Clinical
"We found increased 8(9)-cholestenol and 8-dehydrocholesterol in tissue samples from seven female probands with CDPX2 (ref. 4)."
Documents the accumulated sterols in patient tissue.
Cellular Cholesterol Deficiency
Beyond its structural membrane role, cholesterol is required for the covalent modification and signalling competence of hedgehog proteins, so a block in its synthesis has a developmental-patterning consequence that is separate from any toxicity of the accumulating intermediates. This is the shared explanatory route from a lipid-synthesis enzymopathy to a malformation syndrome across the post-squalene disorders.
smoothened signaling pathway GO:0007224 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves decreased smoothened signaling pathway (GO:0007224). GO:0007224 is a biological process from the Gene Ontology. ↓ DECREASED
Show evidence (1 reference)
PMID:20929975 SUPPORT Other
"Cholesterol is also essential for the proper maturation and signaling of hedgehog proteins, and thus cholesterol is critical for embryonic development."
States the hedgehog-dependence of cholesterol that connects this enzyme class to malformation phenotypes.
X-Inactivation Mosaicism
EBP is on Xp11.22-p11.23, so random X inactivation makes a heterozygous female a functional mosaic of enzyme-competent and enzyme-deficient clones. This node is what converts a uniform enzymopathy into the disorder's signature patterned, asymmetric phenotype, and it is also what accounts for the severity range and for the survival of the rare affected male. It is the mechanistic feature that most sharply distinguishes CDPX2 from the autosomal recessive members of the same skeletal nosology group, where every cell carries the same deficiency.
random X inactivation GO:0060816 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves random X inactivation, annotated with random inactivation of X chromosome (GO:0060816). GO:0060816 is a biological process from the Gene Ontology.
Show evidence (2 references)
PMID:21634086 SUPPORT Human Clinical
"Females who inherit an EBP pathogenic variant will be heterozygotes and have a highly variable clinical phenotype."
Records the variability among heterozygotes that the mosaic model explains.
PMID:24700572 SUPPORT Human Clinical
"These males exhibit a phenotype similar to CDPX2 due to either somatic mosaicism or a 47, XXY karyotype in association with a null EBP allele."
Affected males with a null allele survive only when they are themselves mosaic, which is direct evidence that mosaicism rather than allele identity governs viability.
Abnormal Cartilage Calcification and Endochondral Ossification
Growth-plate and epiphyseal cartilage calcifies abnormally, producing punctate foci of calcification in the epiphyses of long bones, the vertebrae, the trachea, and the costal ends. The stippling is transient - it disappears as the epiphyses ossify - but the disordered endochondral growth it accompanies leaves permanent shortening and spinal deformity.
chondrocyte CL:0000138 Cell Ontology (CL) Relation: this pathophysiological event involves this cell type This pathophysiological event involves chondrocyte (CL:0000138). CL:0000138 is a cell type from the Cell Ontology. growth plate cartilage chondrocyte CL:1000217 Cell Ontology (CL) Relation: this pathophysiological event involves this cell type This pathophysiological event involves growth plate cartilage chondrocyte (CL:1000217). CL:1000217 is a cell type from the Cell Ontology.
endochondral ossification GO:0001958 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves abnormal endochondral ossification (GO:0001958). GO:0001958 is a biological process from the Gene Ontology. ⚠ ABNORMAL cartilage development GO:0051216 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves abnormal cartilage development (GO:0051216). GO:0051216 is a biological process from the Gene Ontology. ⚠ ABNORMAL
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"chondrodysplasia punctata (stippling of the epiphyses of the long bones, vertebrae, trachea, and distal ends of the ribs)"
Enumerates the sites of punctate calcification, which is wider than the epiphyses alone.
Asymmetric Skeletal Involvement
Rhizomelic (proximal) limb shortening in CDPX2 is characteristically unequal between the two sides, giving leg-length discrepancy as well as short stature. The asymmetry is not incidental - it is the skeletal expression of the same clonal mosaic that patterns the skin.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"often asymmetric rhizomelic shortening of limbs"
States that the rhizomelic shortening is characteristically asymmetric.
Blaschkolinear Cutaneous Phenotype
Mutant-expressing keratinocyte clones produce ichthyotic scaling at birth that resolves over infancy into linear and whorled atrophic patches centred on hair follicles, with coarse hair and scarring alopecia in the same distribution. The lesion changes over time while its geometry does not.
keratinocyte CL:0000312 Cell Ontology (CL) Relation: this pathophysiological event involves this cell type This pathophysiological event involves keratinocyte (CL:0000312). CL:0000312 is a cell type from the Cell Ontology.
keratinocyte differentiation GO:0030216 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves abnormal keratinocyte differentiation (GO:0030216). GO:0030216 is a biological process from the Gene Ontology. ⚠ ABNORMAL
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"linear or blotchy scaling ichthyosis in the newborn; later appearance of linear or whorled atrophic patches involving hair follicles (follicular atrophoderma); coarse hair with scarring alopecia; and cataracts."
Describes the evolution of the cutaneous phenotype and its follicular involvement.
Epidermal Barrier and Follicular Dysfunction
The cutaneous phenotype is cell-autonomous to keratinocyte clones expressing the mutant allele, which are the clones that both lack cholesterol and accumulate the upstream sterols. The epidermis is a tissue in which the distal cholesterol pathway is not redundant, since barrier lipids must be synthesized locally.
epidermis development GO:0008544 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves abnormal epidermis development (GO:0008544). GO:0008544 is a biological process from the Gene Ontology. ⚠ ABNORMAL
Male Hemizygous Lethality
A male carrying a null EBP allele on his single X has no enzyme-competent tissue and is typically lost in early gestation. The rule is not absolute, and the two documented escape routes are informative about the mechanism: post-zygotic mosaicism (or a supernumerary X) restores a competent clone population, while a hypomorphic allele leaves residual isomerase activity in every cell.
Show evidence (2 references)
PMID:24700572 SUPPORT Human Clinical
"Mutations in the Emopamil-binding protein (EBP) gene cause X-linked dominant chondrodysplasia punctata 2 (CDPX2), a disorder in which at least 95% of liveborn individuals are female and male intrauterine lethality is assumed."
States the male lethality and the resulting sex ratio among live births.
PMID:24700572 SUPPORT Human Clinical
"Alternatively, affected males may exhibit a distinct phenotype if they are hemizygous for a hypomorphic allele of EBP."
Identifies residual enzyme activity as the second route by which a hemizygous male survives, and notes that the resulting phenotype is not CDPX2.

Pathograph

Use the checkboxes to hide or show graph categories. Hover nodes for evidence and cross-linked metadata.
Pathograph: causal mechanism network for X-linked Chondrodysplasia Punctata 2 Interactive directed graph showing how pathophysiology mechanisms, phenotypes, genetic factors and variants, experimental models, environmental triggers, and treatments relate through causal and linked edges.

Phenotypes

12
Ear 1
Hearing Impairment HP:0000365 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Hearing impairment (HP:0000365). HP:0000365 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"standard interventions for hearing loss and hydronephrosis; family support"
Hearing loss is listed among the manifestations requiring standard intervention.
Eye 1
Cataract HP:0000518 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Cataract (HP:0000518). HP:0000518 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:10391219 SUPPORT Human Clinical
"Additionally, CDPX2 patients may have asymmetric rhizomesomelia, sectorial cataracts, patchy alopecia, ichthyosis and atrophoderma."
Reports sectorial cataracts, the segmental pattern being the mosaic readout.
Head and Neck 1
Scarring Alopecia Scarring alopecia of scalp HP:0004552 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Scarring alopecia of scalp (HP:0004552). HP:0004552 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"coarse hair with scarring alopecia; and cataracts"
Names scarring alopecia as a characteristic feature.
Integument 1
Ichthyosis HP:0008064 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Ichthyosis (HP:0008064), qualified as temporality transient. HP:0008064 is a phenotype from the Human Phenotype Ontology.
Temporal: TRANSIENT
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"linear or blotchy scaling ichthyosis in the newborn"
States the newborn ichthyotic scaling and its linear geometry.
Musculoskeletal 2
Scoliosis HP:0002650 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Scoliosis (HP:0002650), qualified as course progressive. HP:0002650 is a phenotype from the Human Phenotype Ontology.
Course: PROGRESSIVE
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"often asymmetric rhizomelic shortening of limbs; scoliosis; linear or blotchy scaling ichthyosis in the newborn"
Lists scoliosis among the characteristic features.
Coronal Cleft Vertebrae HP:0003417 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Coronal cleft vertebrae (HP:0003417). HP:0003417 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"chondrodysplasia punctata (stippling of the epiphyses of the long bones, vertebrae, trachea, and distal ends of the ribs)"
Vertebral involvement is documented; the specific coronal-cleft morphology is a standard CDP finding but is not itself named in this source, so the support is recorded as partial.
Growth 3
Rhizomelic Limb Shortening Rhizomelia HP:0008905 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Rhizomelia (HP:0008905). HP:0008905 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"often asymmetric rhizomelic shortening of limbs"
Names rhizomelic shortening as a characteristic feature.
Asymmetric Growth HP:0100555 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Asymmetric growth (HP:0100555). HP:0100555 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:10391219 SUPPORT Human Clinical
"Additionally, CDPX2 patients may have asymmetric rhizomesomelia, sectorial cataracts, patchy alopecia, ichthyosis and atrophoderma."
Reports asymmetric rhizomesomelia as a feature of the disorder.
Short Stature HP:0004322 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Short stature (HP:0004322). HP:0004322 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"Characteristic features include growth deficiency; distinctive craniofacial appearance"
Names growth deficiency as a characteristic feature.
Other 3
Epiphyseal Stippling HP:0010655 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Epiphyseal stippling (HP:0010655), qualified as temporality transient. HP:0010655 is a phenotype from the Human Phenotype Ontology.
Temporal: TRANSIENT
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"chondrodysplasia punctata (stippling of the epiphyses of the long bones, vertebrae, trachea, and distal ends of the ribs)"
Names epiphyseal stippling as a characteristic feature and gives its distribution.
Coarse Hair HP:0002208 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Coarse hair (HP:0002208). HP:0002208 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"coarse hair with scarring alopecia; and cataracts"
Names coarse hair as a characteristic feature.
Hydronephrosis HP:0000126 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Hydronephrosis (HP:0000126). HP:0000126 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"monitor hydronephrosis if present per nephrologist"
Hydronephrosis is named as a manifestation requiring surveillance.
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Genetic Associations

1
EBP
Gene: EBP hgnc:3133 HUGO Gene Nomenclature Committee (hgnc) Relation: this disease-associated gene is this gene This disease-associated gene is EBP (hgnc:3133). hgnc:3133 is a gene from the HUGO Gene Nomenclature Committee. relationship_type: CAUSATIVE
Show evidence (2 references)
PMID:10391219 SUPPORT Human Clinical
"A candidate gene encoding a sterol-delta8-isomerase (EBP) has been identified and mapped to Xp11.22-p11.23 (refs 5,6)."
Identifies the gene and its cytogenetic location.
PMID:10391218 SUPPORT Human Clinical
"identified alterations in human EBP in seven unrelated CDPX2 patients."
An independent group identified EBP variants in seven unrelated CDPX2 patients alongside the orthologous mouse mutation, corroborating the gene assignment.
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Medical Actions

3
Orthopedic management and surveillance
Action: supportive careNCI Thesaurus (NCIT) Relation: this treatment is this clinical intervention This treatment is supportive care (NCIT:C15747). NCIT:C15747 is a clinical intervention from the NCI Thesaurus. Ontology label: Supportive Care NCIT:C15747
Management of kyphoscoliosis, joint problems, linear growth, and leg-length discrepancy. No disease-modifying therapy exists; unlike CHILD syndrome, where topical cholesterol plus a statin has been shown to clear the skin, no equivalent pathogenesis-based intervention has been established for CDPX2.
Target Phenotypes: scoliosis HP:0002650 Human Phenotype Ontology (HP) Relation: this treatment targets this phenotype This treatment targets scoliosis (HP:0002650). HP:0002650 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"Orthopedic evaluations to monitor kyphoscoliosis, joint problems, linear growth, and any leg length discrepancy per orthopedist"
States the recommended orthopedic surveillance.
Emollients and keratolytics for the cutaneous phenotype
Action: therapeutic procedureNCI Thesaurus (NCIT) Relation: this treatment is this clinical intervention This treatment is therapeutic procedure (NCIT:C49236). NCIT:C49236 is a clinical intervention from the NCI Thesaurus. Ontology label: Therapeutic Procedure NCIT:C49236
Topical management of the ichthyotic scaling, with sun protection.
Target Phenotypes: ichthyosis HP:0008064 Human Phenotype Ontology (HP) Relation: this treatment targets this phenotype This treatment targets ichthyosis (HP:0008064). HP:0008064 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"dermatologic management with emollients and keratolytics; sun protection"
States the recommended dermatologic management.
Cataract extraction
Action: surgical procedureNCI Thesaurus (NCIT) Relation: this treatment is this clinical intervention This treatment is surgical procedure (NCIT:C15329). NCIT:C15329 is a clinical intervention from the NCI Thesaurus. Ontology label: Surgical Procedure NCIT:C15329
Standard surgical management of the lens opacity.
Target Phenotypes: cataract HP:0000518 Human Phenotype Ontology (HP) Relation: this treatment targets this phenotype This treatment targets cataract (HP:0000518). HP:0000518 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"cataract extraction and standard treatment of ocular manifestations per ophthalmologist"
States the recommended ophthalmologic management.
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Biochemical Markers

1
Plasma and tissue 8(9)-cholestenol and 8-dehydrocholesterol
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"increased concentration of 8(9)-cholestenol and 8-dehydrocholesterol in plasma, scales from skin lesions, or cultured lymphoblasts or fibroblasts"
States the analytes and the acceptable sample types.
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Diagnosis

1
Sterol profiling plus EBP molecular testing
The diagnosis rests on typical clinical findings together with the characteristic sterol elevation and/or an EBP variant. GeneReviews states the criterion separately for female probands (mosaic heterozygous variant) and male probands (mosaic hemizygous variant), which encodes the point that a male reaching diagnosis is expected to be mosaic.
diagnostic procedure NCIT:C18020 NCI Thesaurus (NCIT)
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"The diagnosis of EBP-CDPX is established in a female proband with typical clinical findings; increased concentration of 8(9)-cholestenol and 8-dehydrocholesterol in plasma, scales from skin lesions, or cultured lymphoblasts or fibroblasts; and/or a mosaic heterozygous pathogenic variant in EBP..."
States the diagnostic criteria for a female proband.
📈

Progression

4
Neonatal period - scaling skin and stippled skeleton
The disorder is most recognizable at birth, when both of its diagnostic findings are present simultaneously: patterned ichthyotic scaling and radiographic epiphyseal stippling. Both are transient, which is why a diagnosis missed in the newborn period becomes markedly harder later.
Show evidence (1 reference)
PMID:33147667 SUPPORT Human Clinical
"Herein, we report a full-term newborn that presented at birth with scattered blaschkolinear bands of adherent scales and scalp erosions in a spiral distribution."
Documents the newborn cutaneous presentation and its blaschkolinear geometry.
Infancy and childhood - scaling resolves into follicular atrophoderma
The ichthyotic scaling of the newborn gives way to linear and whorled atrophic patches centred on hair follicles, with coarse hair and scarring alopecia in the same distribution. The skin findings change character but keep the same clonal geometry, because the underlying mosaic is fixed.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"linear or blotchy scaling ichthyosis in the newborn; later appearance of linear or whorled atrophic patches involving hair follicles (follicular atrophoderma); coarse hair with scarring alopecia; and cataracts."
States the sequence from newborn scaling to follicular atrophoderma and scarring alopecia.
Childhood to adulthood - orthopedic course
Epiphyseal stippling resolves as the epiphyses ossify, but the asymmetric limb shortening it accompanied does not, and scoliosis becomes the dominant orthopedic problem. Surveillance is therefore orthopedic and ophthalmologic rather than dermatologic in later life.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"Orthopedic evaluations to monitor kyphoscoliosis, joint problems, linear growth, and any leg length discrepancy per orthopedist"
The recommended surveillance identifies kyphoscoliosis and leg-length discrepancy as the persisting skeletal problems.
Whole-lifespan severity range
CDPX2 has no single natural history. The same gene, and sometimes the same variant within one family, produces outcomes from fetal demise to an adult with no recognizable abnormality, because the phenotype is a function of the mosaic proportion rather than of the allele alone.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"The findings in EBP-related X-linked chondrodysplasia punctata (EBP-CDPX) range from fetal demise with multiple malformations and severe growth deficiency to much milder manifestations, including females with no recognizable physical abnormalities."
States the full severity range directly.
📊

Prevalence

2
Live-born affected individuals, sex distribution
Unknown Unknown
No population prevalence estimate is cited here. What the literature does quantify is the sex ratio among live births, which is itself a consequence of the mechanism (early gestational lethality in hemizygous males).
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"At least 95% of live-born individuals with EBP-CDPX are female."
Quantifies the sex distribution among live births that follows from male hemizygous lethality.
Affected females, proportion with a de novo variant
Unknown Unknown
A recurrence-risk rather than an occurrence figure, recorded here because it is the number that drives counselling: most affected females are simplex cases, but a substantial minority have a mildly affected or mosaic parent.
Show evidence (1 reference)
PMID:21634086 SUPPORT Human Clinical
"About 60% of affected females have the disorder as the result of a de novo pathogenic variant."
Establishes that roughly 40% inherit the variant, which is why parental evaluation is part of the diagnostic workup.
🔀

Differential Diagnoses

3

Conditions with similar clinical presentations that must be differentiated from X-linked Chondrodysplasia Punctata 2:

Overlapping Features The nearest mechanistic neighbour: also X-linked, also male-lethal, also a mosaic disorder of the post-squalene cholesterol pathway, and also placed in ISDS group 23. NSDHL acts one step upstream of EBP. The two are separated clinically by lesion geometry rather than by pathway.
Distinguishing Features
  • CHILD syndrome lesions are strictly lateralized with a sharp body-midline boundary; CDPX2 lesions are bilateral, asymmetric, and follow Blaschko lines
  • CDPX2 carries elevated 8(9)-cholestenol and 8-dehydrocholesterol; NSDHL-related CHILD syndrome carries a methylsterol pattern instead
  • CHILD syndrome has ipsilateral limb reduction defects; CDPX2 has asymmetric rhizomelic shortening without true reduction defects
Overlapping Features The other major cause of a stippled, rhizomelic newborn skeleton, and the principal radiographic differential. It is autosomal recessive and peroxisomal rather than X-linked and sterol-pathway, and every cell is uniformly affected.
Distinguishing Features
  • RCDP1 limb shortening is symmetric; CDPX2 shortening is characteristically asymmetric
  • RCDP1 has profound developmental impairment and seizures; CDPX2 intellect is usually normal
  • RCDP1 is confirmed by erythrocyte plasmalogen deficiency with raised phytanic acid; CDPX2 by the 8(9)-cholestenol / 8-dehydrocholesterol sterol profile
  • RCDP1 affects both sexes equally; at least 95% of live-born CDPX2 patients are female
Overlapping Features The X-linked recessive CDP, affecting males, with nasomaxillary hypoplasia and distal phalangeal shortening rather than ichthyosis and rhizomelia. Its vitamin-K-related phenocopies (maternal warfarin exposure, maternal autoimmune disease) share the same radiographic pattern and must be excluded in a stippled newborn before either genetic diagnosis is made.
Distinguishing Features
  • CDPX1 affects males; CDPX2 live births are overwhelmingly female
  • CDPX1 has brachytelephalangy and nasal hypoplasia without ichthyosis, alopecia, or cataract
  • CDPX1 has a normal sterol profile
🐁

Animal Models

1
Tattered (Td) mouse
A spontaneous X-linked semi-dominant mouse mutation that was independently mapped to Ebp, giving a naturally occurring orthologue of CDPX2 in which the mosaic logic of the disease is directly visible: heterozygous females develop hairless hyperkeratotic patches that read out as coat striping in the adult, and hemizygous males die prenatally.
Species
Mouse
Genotype
Ebp missense substitution, X-linked semi-dominant (Td), heterozygous females
Publication
{ }

Source YAML

click to show
name: X-linked Chondrodysplasia Punctata 2
creation_date: "2026-08-27T00:00:00Z"
category: Mendelian
description: >-
  X-linked dominant chondrodysplasia punctata type 2 (CDPX2, Conradi-Hunermann-Happle
  syndrome) is caused by heterozygous loss-of-function variants in EBP, which encodes
  the 3-beta-hydroxysteroid-delta8,delta7-isomerase (emopamil-binding protein) that
  isomerizes the double bond one step below lanosterol in post-squalene cholesterol
  biosynthesis. As in the other distal-cholesterol-pathway disorders the lesion is
  dual: cells lose the pathway end product and simultaneously accumulate the
  immediately upstream intermediates, 8(9)-cholestenol and 8-dehydrocholesterol, whose
  elevation in plasma, skin scale, or cultured cells is the biochemical signature of
  the disease. What makes CDPX2 mechanistically distinctive within its ISDS nosology
  group is not the enzymology but the fact that the phenotype is a *mosaic* readout of
  it. EBP is X-linked, so a heterozygous female is a functional mosaic of
  enzyme-competent and enzyme-deficient clones, and the disease is written onto the
  body in that clonal geometry - blaschkolinear ichthyotic scaling in the newborn that
  later resolves into linear follicular atrophoderma and scarring alopecia along the
  same lines, asymmetric rather than symmetric rhizomelic shortening, sectorial rather
  than whole-lens cataract, and side-to-side differences in epiphyseal stippling. The
  same mosaicism explains the disorder's unusual epidemiology at both extremes: at
  least 95% of live-born affected individuals are female because hemizygous males are
  usually lost in early gestation, while the affected males who do survive do so by
  being mosaic themselves (post-zygotic variants or a 47,XXY karyotype) or by carrying
  a hypomorphic allele that leaves residual isomerase activity. Because clone
  proportions vary between individuals and between body regions, the severity range is
  correspondingly enormous - from fetal demise with multiple malformations to obligate
  carrier mothers whose only finding is a strand of atrophic scalp skin, recognized
  only after a severely affected daughter is born.
synonyms:
- CDPX2
- Conradi-Hunermann-Happle syndrome
- Conradi-Hunermann syndrome
- X-linked dominant chondrodysplasia punctata
- EBP-related X-linked chondrodysplasia punctata
- EBP-CDPX
disease_term:
  preferred_term: X-linked chondrodysplasia punctata 2
  term:
    id: MONDO:0020603
    label: X-linked chondrodysplasia punctata 2
parents:
- X-linked Chondrodysplasia Punctata
- Sterol Biosynthesis Disorder
- Hereditary Skin Disorder
classifications:
  harrisons_chapter:
  - classification_value: GENETICS_ENVIRONMENT_DISEASE
    notes: >-
      An X-linked Mendelian disorder whose entire clinical logic - male lethality, the
      mosaic distribution of lesions, the severity range between relatives carrying the
      same allele - is genetic rather than organ-system based.
  - classification_value: DERMATOLOGY
    notes: >-
      The newborn presentation is cutaneous, and the linear ichthyosis, follicular
      atrophoderma, and scarring alopecia are the findings that persist and that most
      often bring an adult carrier to attention.
  - classification_value: ENDOCRINOLOGY_METABOLISM
    notes: >-
      Also an inborn error of metabolism: an enzymatic block in post-squalene
      cholesterol biosynthesis, confirmed by the plasma or tissue sterol profile.
  icimd_category:
  - classification_value: sterol_metabolism
    notes: >-
      ICIMD (Ferreira et al. 2021, PMID:33340416): group "Disorders of sterol
      metabolism" under category "Disorders of lipid metabolism". EBP is the
      sterol-delta8-isomerase of the distal cholesterol pathway, placing CDPX2 with
      CHILD syndrome, Smith-Lemli-Opitz syndrome, and the other post-squalene defects
      rather than with the peroxisomal members of its skeletal nosology group.
  isds_skeletal_category:
  - classification_value: chondrodysplasia_punctata
    notes: >-
      ISDS Nosology of Genetic Skeletal Disorders, 2023 revision (Unger et al.,
      PMID:36779427), group 23 "Chondrodysplasia punctata (CDP) group", row
      NOS 23-0020 "CDP, X-linked dominant, EBP-related (Conradi-Hunermann type; CDPX2)"
      (MIM 302960). Carried forward from the 2019 revision (Mortier et al.,
      PMID:31633310), where the group was numbered 21. Exactly one group is assigned:
      the nosology lists each entity once, and CDPX2's sterol-pathway biology - shared
      with CHILD syndrome and Greenberg dysplasia, which sit in the same group, but
      also with Smith-Lemli-Opitz syndrome, which is not a nosology entity at all - is
      handled by the table's cross-references, not by dual membership.
mappings:
  mondo_mappings:
  - term:
      id: MONDO:0020603
      label: X-linked chondrodysplasia punctata 2
    mapping_predicate: skos:exactMatch
    mapping_source: MONDO
    mapping_justification: >-
      The dismech entry and the MONDO class denote the same entity: EBP-related
      X-linked dominant chondrodysplasia punctata. The entry additionally discusses
      the hypomorphic-allele male phenotype, which some authors argue is a distinct
      X-linked recessive entity; that is recorded as a discussion rather than folded
      into the mapping.
references:
- reference: PMID:21634086
  title: "EBP-Related X-Linked Chondrodysplasia Punctata."
  tags:
  - GeneReviews
- reference: PMID:10391219
  title: "Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hünermann syndrome."
- reference: PMID:10391218
  title: "Mutations in a delta 8-delta 7 sterol isomerase in the tattered mouse and X-linked dominant chondrodysplasia punctata. jderry@immunex.com."
- reference: PMID:24700572
  title: "A novel EBP c.224T>A mutation supports the existence of a male-specific disorder independent of CDPX2."
- reference: PMID:20929975
  title: "Malformation syndromes caused by disorders of cholesterol synthesis."
prevalence:
- population: Live-born affected individuals, sex distribution
  measure_type: UNKNOWN
  prevalence_class: UNKNOWN
  notes: >-
    No population prevalence estimate is cited here. What the literature does quantify
    is the sex ratio among live births, which is itself a consequence of the mechanism
    (early gestational lethality in hemizygous males).
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "At least 95% of live-born individuals with EBP-CDPX are female."
    explanation: >-
      Quantifies the sex distribution among live births that follows from male
      hemizygous lethality.
- population: Affected females, proportion with a de novo variant
  measure_type: UNKNOWN
  prevalence_class: UNKNOWN
  notes: >-
    A recurrence-risk rather than an occurrence figure, recorded here because it is the
    number that drives counselling: most affected females are simplex cases, but a
    substantial minority have a mildly affected or mosaic parent.
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "About 60% of affected females have the disorder as the result of a de novo pathogenic variant."
    explanation: >-
      Establishes that roughly 40% inherit the variant, which is why parental
      evaluation is part of the diagnostic workup.
progression:
- phase: Neonatal period - scaling skin and stippled skeleton
  notes: >-
    The disorder is most recognizable at birth, when both of its diagnostic findings
    are present simultaneously: patterned ichthyotic scaling and radiographic
    epiphyseal stippling. Both are transient, which is why a diagnosis missed in the
    newborn period becomes markedly harder later.
  evidence:
  - reference: PMID:33147667
    reference_title: "Conradi-Hünermann-Happle syndrome: report of a novel heterozygous mutation on the emopamil-binding protein gene, c.333delC."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Herein, we report a full-term newborn that presented at birth with scattered
      blaschkolinear bands of adherent scales and scalp erosions in a spiral
      distribution.
    explanation: >-
      Documents the newborn cutaneous presentation and its blaschkolinear geometry.
- phase: Infancy and childhood - scaling resolves into follicular atrophoderma
  notes: >-
    The ichthyotic scaling of the newborn gives way to linear and whorled atrophic
    patches centred on hair follicles, with coarse hair and scarring alopecia in the
    same distribution. The skin findings change character but keep the same clonal
    geometry, because the underlying mosaic is fixed.
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      linear or blotchy scaling ichthyosis in the newborn; later appearance of linear
      or whorled atrophic patches involving hair follicles (follicular atrophoderma);
      coarse hair with scarring alopecia; and cataracts.
    explanation: >-
      States the sequence from newborn scaling to follicular atrophoderma and scarring
      alopecia.
- phase: Childhood to adulthood - orthopedic course
  notes: >-
    Epiphyseal stippling resolves as the epiphyses ossify, but the asymmetric limb
    shortening it accompanied does not, and scoliosis becomes the dominant orthopedic
    problem. Surveillance is therefore orthopedic and ophthalmologic rather than
    dermatologic in later life.
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Orthopedic evaluations to monitor kyphoscoliosis, joint problems, linear growth,
      and any leg length discrepancy per orthopedist
    explanation: >-
      The recommended surveillance identifies kyphoscoliosis and leg-length discrepancy
      as the persisting skeletal problems.
- phase: Whole-lifespan severity range
  notes: >-
    CDPX2 has no single natural history. The same gene, and sometimes the same variant
    within one family, produces outcomes from fetal demise to an adult with no
    recognizable abnormality, because the phenotype is a function of the mosaic
    proportion rather than of the allele alone.
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The findings in EBP-related X-linked chondrodysplasia punctata (EBP-CDPX) range
      from fetal demise with multiple malformations and severe growth deficiency to
      much milder manifestations, including females with no recognizable physical
      abnormalities.
    explanation: >-
      States the full severity range directly.
pathophysiology:
- name: EBP Sterol-Delta8-Isomerase Deficiency
  biological_scale: MOLECULAR
  description: >-
    Loss-of-function variants in EBP abolish or reduce
    3-beta-hydroxysteroid-delta8,delta7-isomerase activity, the step that moves the
    sterol double bond from position 8 to position 7 in the conversion of lanosterol to
    cholesterol. The enzymatic assignment was made in the reverse of the usual
    direction: the sterol profile of patients was characterized first and pointed at
    the isomerase, and the candidate gene was then found mutated in every proband
    tested.
  genetic_context:
    functional_impact_category: LOSS_OF_FUNCTION
    variant_origin: GERMLINE
    zygosity: HETEROZYGOUS
    gene:
      preferred_term: EBP
      term:
        id: hgnc:3133
        label: EBP
    description: >-
      Heterozygous germline (or, in the surviving affected male, post-zygotic mosaic)
      loss-of-function variants. Missense alleles were shown to be functionally
      deficient by complementation in an isomerase-deficient yeast strain. Zygosity is
      recorded as heterozygous because that is the state in the affected female who
      constitutes at least 95% of live births; a hemizygous male with a null allele is
      generally not viable.
  molecular_functions:
  - preferred_term: sterol delta8-delta7 isomerase activity
    modifier: LOSS_OF_FUNCTION
    term:
      id: GO:0000247
      label: C-8 sterol isomerase activity
  biological_processes:
  - preferred_term: cholesterol biosynthesis
    modifier: DECREASED
    term:
      id: GO:0006695
      label: cholesterol biosynthetic process
  evidence:
  - reference: PMID:10391219
    reference_title: "Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hünermann syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Using SSCP analysis and sequencing of genomic DNA, we found EBP mutations in all probands."
    explanation: >-
      Establishes EBP variants as the cause in the biochemically defined patient
      cohort.
  - reference: PMID:10391219
    reference_title: "Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hünermann syndrome."
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      We confirmed the functional significance of two missense alleles by expressing
      them in a sterol-delta8-isomerase-deficient yeast strain.
    explanation: >-
      A complementation assay in an isomerase-deficient yeast strain establishes that
      the patient missense alleles are functionally deficient, not merely associated.
  downstream:
  - target: Accumulation of 8(9)-Cholestenol and 8-Dehydrocholesterol
    description: >-
      Blocking the isomerase backs up flux immediately above the block, so the two
      substrate sterols accumulate in plasma and tissue.
    causal_link_type: DIRECT
    evidence:
    - reference: PMID:10391219
      reference_title: "Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hünermann syndrome."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        This pattern of accumulated cholesterol intermediates suggested a deficiency of
        3beta-hydroxysteroid-delta8,delta7-isomerase (sterol-delta8-isomerase), which
        catalyses an intermediate step in the conversion of lanosterol to cholesterol.
      explanation: >-
        Explicitly links the accumulated intermediates to the position of the enzymatic
        block.
  - target: Cellular Cholesterol Deficiency
    description: >-
      The same block starves the cell of the pathway end product. Both arms of the
      lesion operate together, which is the general pattern of the post-squalene
      cholesterol-synthesis disorders.
    causal_link_type: DIRECT
    evidence:
    - reference: PMID:20929975
      reference_title: "Malformation syndromes caused by disorders of cholesterol synthesis."
      supports: SUPPORT
      evidence_source: OTHER
      snippet: >-
        Generally, inborn errors of cholesterol synthesis lead to both a deficiency of
        cholesterol and increased levels of potentially bioactive or toxic precursor
        sterols.
      explanation: >-
        States the dual lesion that applies to this class of disorders, of which CDPX2
        is one of the reviewed members.
- name: Accumulation of 8(9)-Cholestenol and 8-Dehydrocholesterol
  biological_scale: MOLECULAR
  description: >-
    The two sterols immediately upstream of the isomerase accumulate. Their elevation
    is both the diagnostic marker of the disease and, in the shared model for this
    disorder class, a potentially bioactive or toxic insult in its own right rather
    than an inert by-product. Because the accumulation occurs only in
    mutant-expressing clones, it is a cell-autonomous signal that tracks the mosaic.
  evidence:
  - reference: PMID:10391219
    reference_title: "Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hünermann syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      We found increased 8(9)-cholestenol and 8-dehydrocholesterol in tissue samples
      from seven female probands with CDPX2 (ref. 4).
    explanation: >-
      Documents the accumulated sterols in patient tissue.
  downstream:
  - target: Abnormal Cartilage Calcification and Endochondral Ossification
    description: >-
      Sterol-pathway disruption in growth-plate and epiphyseal cartilage produces the
      punctate calcification that names the disorder family.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:10391219
      reference_title: "Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hünermann syndrome."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Our results indicate that defects in sterol-delta8-isomerase cause CDPX2 and
        suggest a role for sterols in bone development.
      explanation: >-
        The authors themselves frame the connection between the enzyme defect and bone
        development as a suggestion, which is why this edge is annotated as having
        unknown intermediates.
  - target: Epidermal Barrier and Follicular Dysfunction
    description: >-
      Keratinocytes depend on the distal cholesterol pathway for barrier lipid
      synthesis, and the mutant-expressing clones show the ichthyotic and follicular
      phenotype.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
- name: Cellular Cholesterol Deficiency
  biological_scale: CELLULAR
  description: >-
    Beyond its structural membrane role, cholesterol is required for the covalent
    modification and signalling competence of hedgehog proteins, so a block in its
    synthesis has a developmental-patterning consequence that is separate from any
    toxicity of the accumulating intermediates. This is the shared explanatory route
    from a lipid-synthesis enzymopathy to a malformation syndrome across the
    post-squalene disorders.
  biological_processes:
  - preferred_term: smoothened signaling pathway
    modifier: DECREASED
    term:
      id: GO:0007224
      label: smoothened signaling pathway
  evidence:
  - reference: PMID:20929975
    reference_title: "Malformation syndromes caused by disorders of cholesterol synthesis."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: >-
      Cholesterol is also essential for the proper maturation and signaling of hedgehog
      proteins, and thus cholesterol is critical for embryonic development.
    explanation: >-
      States the hedgehog-dependence of cholesterol that connects this enzyme class to
      malformation phenotypes.
  downstream:
  - target: Abnormal Cartilage Calcification and Endochondral Ossification
    description: >-
      Hedgehog signalling governs growth-plate chondrocyte proliferation and
      differentiation, so its impairment is a plausible route from the enzyme block to
      the skeletal phenotype.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
- name: X-Inactivation Mosaicism
  biological_scale: ORGANISM
  description: >-
    EBP is on Xp11.22-p11.23, so random X inactivation makes a heterozygous female a
    functional mosaic of enzyme-competent and enzyme-deficient clones. This node is
    what converts a uniform enzymopathy into the disorder's signature patterned,
    asymmetric phenotype, and it is also what accounts for the severity range and for
    the survival of the rare affected male. It is the mechanistic feature that most
    sharply distinguishes CDPX2 from the autosomal recessive members of the same
    skeletal nosology group, where every cell carries the same deficiency.
  biological_processes:
  - preferred_term: random X inactivation
    term:
      id: GO:0060816
      label: random inactivation of X chromosome
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Females who inherit an EBP pathogenic variant will be heterozygotes and have a
      highly variable clinical phenotype.
    explanation: >-
      Records the variability among heterozygotes that the mosaic model explains.
  - reference: PMID:24700572
    reference_title: "A novel EBP c.224T>A mutation supports the existence of a male-specific disorder independent of CDPX2."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      These males exhibit a phenotype similar to CDPX2 due to either somatic mosaicism
      or a 47, XXY karyotype in association with a null EBP allele.
    explanation: >-
      Affected males with a null allele survive only when they are themselves mosaic,
      which is direct evidence that mosaicism rather than allele identity governs
      viability.
  downstream:
  - target: Blaschkolinear Cutaneous Phenotype
    description: >-
      Clones of mutant-expressing keratinocytes follow the embryonic migration paths of
      the epidermis, so their dysfunction is written on the skin as Blaschko lines.
    causal_link_type: DIRECT
    evidence:
    - reference: PMID:33147667
      reference_title: "Conradi-Hünermann-Happle syndrome: report of a novel heterozygous mutation on the emopamil-binding protein gene, c.333delC."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Herein, we report a full-term newborn that presented at birth with scattered
        blaschkolinear bands of adherent scales and scalp erosions in a spiral
        distribution.
      explanation: >-
        The blaschkolinear and spiral distribution of the lesions is the visible
        expression of the clonal mosaic.
  - target: Asymmetric Skeletal Involvement
    description: >-
      Because the mosaic is not bilaterally symmetric, limb shortening and epiphyseal
      stippling differ between the two sides - a pattern that separates CDPX2 from the
      symmetric rhizomelic CDPs.
    causal_link_type: DIRECT
    evidence:
    - reference: PMID:10391219
      reference_title: "Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hünermann syndrome."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Additionally, CDPX2 patients may have asymmetric rhizomesomelia, sectorial
        cataracts, patchy alopecia, ichthyosis and atrophoderma.
      explanation: >-
        The asymmetry of the limb shortening, the sectorial rather than total cataract,
        and the patchiness of the alopecia are all mosaic readouts listed together.
  - target: Male Hemizygous Lethality
    description: >-
      A non-mosaic hemizygous male has no enzyme-competent clones anywhere, and is
      usually lost in early gestation.
    causal_link_type: DIRECT
    evidence:
    - reference: PMID:21634086
      reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        EBP-CDPX is inherited in an X-linked manner with typical (but not absolute)
        early gestational male lethality.
      explanation: >-
        States the male lethality and, in the parenthesis, that it is not absolute -
        the escape routes being mosaicism and hypomorphic alleles.
- name: Abnormal Cartilage Calcification and Endochondral Ossification
  biological_scale: TISSUE
  description: >-
    Growth-plate and epiphyseal cartilage calcifies abnormally, producing punctate foci
    of calcification in the epiphyses of long bones, the vertebrae, the trachea, and
    the costal ends. The stippling is transient - it disappears as the epiphyses
    ossify - but the disordered endochondral growth it accompanies leaves permanent
    shortening and spinal deformity.
  cell_types:
  - preferred_term: chondrocyte
    term:
      id: CL:0000138
      label: chondrocyte
  - preferred_term: growth plate cartilage chondrocyte
    term:
      id: CL:1000217
      label: growth plate cartilage chondrocyte
  biological_processes:
  - preferred_term: endochondral ossification
    modifier: ABNORMAL
    term:
      id: GO:0001958
      label: endochondral ossification
  - preferred_term: cartilage development
    modifier: ABNORMAL
    term:
      id: GO:0051216
      label: cartilage development
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      chondrodysplasia punctata (stippling of the epiphyses of the long bones,
      vertebrae, trachea, and distal ends of the ribs)
    explanation: >-
      Enumerates the sites of punctate calcification, which is wider than the epiphyses
      alone.
  downstream:
  - target: Epiphyseal Stippling
    causal_link_type: DIRECT
    description: Punctate calcification in cartilage is the defining radiographic finding.
  - target: Coronal Cleft Vertebrae
    causal_link_type: DIRECT
    description: Vertebral cartilage involvement produces coronal clefting of the vertebral bodies.
  - target: Scoliosis
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    intermediate_mechanisms:
    - Asymmetric vertebral growth following disordered endochondral ossification.
    description: >-
      Spinal deformity develops out of the disordered and asymmetric vertebral growth
      and is the orthopedic problem that persists after the stippling resolves.
  - target: Short Stature
    causal_link_type: DIRECT
    description: Disordered endochondral growth limits linear growth.
- name: Asymmetric Skeletal Involvement
  biological_scale: ORGANISM
  description: >-
    Rhizomelic (proximal) limb shortening in CDPX2 is characteristically unequal
    between the two sides, giving leg-length discrepancy as well as short stature. The
    asymmetry is not incidental - it is the skeletal expression of the same clonal
    mosaic that patterns the skin.
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "often asymmetric rhizomelic shortening of limbs"
    explanation: >-
      States that the rhizomelic shortening is characteristically asymmetric.
  downstream:
  - target: Rhizomelic Limb Shortening
    causal_link_type: DIRECT
    description: Proximal limb segments are disproportionately short.
  - target: Asymmetric Growth
    causal_link_type: DIRECT
    description: The unequal involvement of the two sides produces measurable asymmetry and leg-length discrepancy.
- name: Blaschkolinear Cutaneous Phenotype
  biological_scale: TISSUE
  description: >-
    Mutant-expressing keratinocyte clones produce ichthyotic scaling at birth that
    resolves over infancy into linear and whorled atrophic patches centred on hair
    follicles, with coarse hair and scarring alopecia in the same distribution. The
    lesion changes over time while its geometry does not.
  cell_types:
  - preferred_term: keratinocyte
    term:
      id: CL:0000312
      label: keratinocyte
  biological_processes:
  - preferred_term: keratinocyte differentiation
    modifier: ABNORMAL
    term:
      id: GO:0030216
      label: keratinocyte differentiation
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      linear or blotchy scaling ichthyosis in the newborn; later appearance of linear
      or whorled atrophic patches involving hair follicles (follicular atrophoderma);
      coarse hair with scarring alopecia; and cataracts.
    explanation: >-
      Describes the evolution of the cutaneous phenotype and its follicular
      involvement.
  downstream:
  - target: Ichthyosis
    causal_link_type: DIRECT
    description: The newborn presentation is linear or blotchy ichthyotic scaling.
  - target: Scarring Alopecia
    causal_link_type: DIRECT
    description: Follicular destruction within affected clones produces patchy scarring alopecia.
  - target: Coarse Hair
    causal_link_type: DIRECT
    description: Hair shafts arising from affected follicles are coarse and lustreless.
- name: Epidermal Barrier and Follicular Dysfunction
  biological_scale: CELLULAR
  description: >-
    The cutaneous phenotype is cell-autonomous to keratinocyte clones expressing the
    mutant allele, which are the clones that both lack cholesterol and accumulate the
    upstream sterols. The epidermis is a tissue in which the distal cholesterol pathway
    is not redundant, since barrier lipids must be synthesized locally.
  biological_processes:
  - preferred_term: epidermis development
    modifier: ABNORMAL
    term:
      id: GO:0008544
      label: epidermis development
  downstream:
  - target: Blaschkolinear Cutaneous Phenotype
    causal_link_type: DIRECT
    description: Clone-restricted keratinocyte dysfunction appears as the patterned cutaneous phenotype.
- name: Male Hemizygous Lethality
  biological_scale: ORGANISM
  description: >-
    A male carrying a null EBP allele on his single X has no enzyme-competent tissue
    and is typically lost in early gestation. The rule is not absolute, and the two
    documented escape routes are informative about the mechanism: post-zygotic
    mosaicism (or a supernumerary X) restores a competent clone population, while a
    hypomorphic allele leaves residual isomerase activity in every cell.
  evidence:
  - reference: PMID:24700572
    reference_title: "A novel EBP c.224T>A mutation supports the existence of a male-specific disorder independent of CDPX2."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Mutations in the Emopamil-binding protein (EBP) gene cause X-linked dominant
      chondrodysplasia punctata 2 (CDPX2), a disorder in which at least 95% of liveborn
      individuals are female and male intrauterine lethality is assumed.
    explanation: >-
      States the male lethality and the resulting sex ratio among live births.
  - reference: PMID:24700572
    reference_title: "A novel EBP c.224T>A mutation supports the existence of a male-specific disorder independent of CDPX2."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Alternatively, affected males may exhibit a distinct phenotype if they are
      hemizygous for a hypomorphic allele of EBP.
    explanation: >-
      Identifies residual enzyme activity as the second route by which a hemizygous
      male survives, and notes that the resulting phenotype is not CDPX2.
phenotypes:
- name: Epiphyseal Stippling
  category: Skeletal
  description: >-
    Punctate foci of calcification in epiphyseal cartilage, and also in the vertebrae,
    trachea, and distal ribs. Present at birth and resolving through infancy.
  phenotype_term:
    preferred_term: Epiphyseal stippling
    term:
      id: HP:0010655
      label: Epiphyseal stippling
    temporality: TRANSIENT
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      chondrodysplasia punctata (stippling of the epiphyses of the long bones,
      vertebrae, trachea, and distal ends of the ribs)
    explanation: Names epiphyseal stippling as a characteristic feature and gives its distribution.
- name: Rhizomelic Limb Shortening
  category: Skeletal
  description: >-
    Shortening of the proximal limb segments, characteristically unequal between the
    two sides.
  phenotype_term:
    preferred_term: Rhizomelia
    term:
      id: HP:0008905
      label: Rhizomelia
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "often asymmetric rhizomelic shortening of limbs"
    explanation: Names rhizomelic shortening as a characteristic feature.
- name: Asymmetric Growth
  category: Skeletal
  description: >-
    Unequal involvement of the two sides of the body, producing leg-length discrepancy
    and asymmetric limb shortening - the skeletal signature of the underlying mosaic.
  phenotype_term:
    preferred_term: Asymmetric growth
    term:
      id: HP:0100555
      label: Asymmetric growth
  evidence:
  - reference: PMID:10391219
    reference_title: "Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hünermann syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Additionally, CDPX2 patients may have asymmetric rhizomesomelia, sectorial
      cataracts, patchy alopecia, ichthyosis and atrophoderma.
    explanation: Reports asymmetric rhizomesomelia as a feature of the disorder.
- name: Scoliosis
  category: Skeletal
  description: >-
    Lateral spinal curvature, frequently with a kyphotic component, developing out of
    asymmetric vertebral growth and persisting as the main orthopedic problem.
  phenotype_term:
    preferred_term: Scoliosis
    term:
      id: HP:0002650
      label: Scoliosis
    clinical_course: PROGRESSIVE
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      often asymmetric rhizomelic shortening of limbs; scoliosis; linear or blotchy
      scaling ichthyosis in the newborn
    explanation: Lists scoliosis among the characteristic features.
- name: Coronal Cleft Vertebrae
  category: Skeletal
  description: Coronal clefting of the vertebral bodies, a cartilage-maturation finding shared across the CDP family.
  phenotype_term:
    preferred_term: Coronal cleft vertebrae
    term:
      id: HP:0003417
      label: Coronal cleft vertebrae
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      chondrodysplasia punctata (stippling of the epiphyses of the long bones,
      vertebrae, trachea, and distal ends of the ribs)
    explanation: >-
      Vertebral involvement is documented; the specific coronal-cleft morphology is a
      standard CDP finding but is not itself named in this source, so the support is
      recorded as partial.
- name: Short Stature
  category: Growth
  description: Growth deficiency, present prenatally in the severe end of the spectrum.
  phenotype_term:
    preferred_term: Short stature
    term:
      id: HP:0004322
      label: Short stature
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Characteristic features include growth deficiency; distinctive craniofacial appearance"
    explanation: Names growth deficiency as a characteristic feature.
- name: Ichthyosis
  category: Dermatologic
  description: >-
    Linear or blotchy adherent scaling present in the newborn period, distributed along
    Blaschko lines, and resolving over the first months.
  phenotype_term:
    preferred_term: Ichthyosis
    term:
      id: HP:0008064
      label: Ichthyosis
    temporality: TRANSIENT
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "linear or blotchy scaling ichthyosis in the newborn"
    explanation: States the newborn ichthyotic scaling and its linear geometry.
- name: Scarring Alopecia
  category: Dermatologic
  description: >-
    Patchy cicatricial alopecia following follicular destruction within
    mutant-expressing clones, in the same linear distribution as the earlier scaling.
  phenotype_term:
    preferred_term: Scarring alopecia of scalp
    term:
      id: HP:0004552
      label: Scarring alopecia of scalp
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "coarse hair with scarring alopecia; and cataracts"
    explanation: Names scarring alopecia as a characteristic feature.
- name: Coarse Hair
  category: Dermatologic
  description: Coarse, lustreless hair arising from affected follicles.
  phenotype_term:
    preferred_term: Coarse hair
    term:
      id: HP:0002208
      label: Coarse hair
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "coarse hair with scarring alopecia; and cataracts"
    explanation: Names coarse hair as a characteristic feature.
- name: Cataract
  category: Ophthalmologic
  description: >-
    Lens opacity, characteristically sectorial rather than whole-lens - itself a mosaic
    readout, since only lens fibre clones expressing the mutant allele are affected.
  phenotype_term:
    preferred_term: Cataract
    term:
      id: HP:0000518
      label: Cataract
  evidence:
  - reference: PMID:10391219
    reference_title: "Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hünermann syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Additionally, CDPX2 patients may have asymmetric rhizomesomelia, sectorial
      cataracts, patchy alopecia, ichthyosis and atrophoderma.
    explanation: Reports sectorial cataracts, the segmental pattern being the mosaic readout.
- name: Hearing Impairment
  category: Otologic
  description: Hearing loss occurs and is an explicit target of surveillance and standard intervention.
  phenotype_term:
    preferred_term: Hearing impairment
    term:
      id: HP:0000365
      label: Hearing impairment
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "standard interventions for hearing loss and hydronephrosis; family support"
    explanation: Hearing loss is listed among the manifestations requiring standard intervention.
- name: Hydronephrosis
  category: Renal
  description: Hydronephrosis occurs in a subset and is monitored when present.
  phenotype_term:
    preferred_term: Hydronephrosis
    term:
      id: HP:0000126
      label: Hydronephrosis
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "monitor hydronephrosis if present per nephrologist"
    explanation: Hydronephrosis is named as a manifestation requiring surveillance.
genetic:
- name: EBP
  relationship_type: CAUSATIVE
  presence: Present
  gene_term:
    preferred_term: EBP
    term:
      id: hgnc:3133
      label: EBP
  notes: >-
    EBP encodes 3-beta-hydroxysteroid-delta8,delta7-isomerase (emopamil-binding
    protein), mapped to Xp11.22-p11.23. Pathogenic variants span missense, nonsense,
    frameshift, and splice classes; the missense alleles have been shown to be
    functionally deficient by complementation in an isomerase-deficient yeast strain
    rather than inferred from prediction alone.
  evidence:
  - reference: PMID:10391219
    reference_title: "Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hünermann syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      A candidate gene encoding a sterol-delta8-isomerase (EBP) has been identified and
      mapped to Xp11.22-p11.23 (refs 5,6).
    explanation: Identifies the gene and its cytogenetic location.
  - reference: PMID:10391218
    reference_title: "Mutations in a delta 8-delta 7 sterol isomerase in the tattered mouse and X-linked dominant chondrodysplasia punctata. jderry@immunex.com."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      identified alterations in human EBP in seven unrelated CDPX2
      patients.
    explanation: >-
      An independent group identified EBP variants in seven unrelated CDPX2 patients
      alongside the orthologous mouse mutation, corroborating the gene assignment.
inheritance:
- name: X-linked dominant inheritance with male lethality
  inheritance_term:
    preferred_term: X-linked dominant inheritance
    term:
      id: HP:0001423
      label: X-linked dominant inheritance
  description: >-
    Inherited in an X-linked manner with early gestational lethality in hemizygous
    males. About 60% of affected females carry a de novo variant; the remainder inherit
    it from a mildly affected mother or from a somatically/gonadally mosaic father. A
    female who inherits a variant is a heterozygote whose severity cannot be predicted
    from the genotype.
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      EBP-CDPX is inherited in an X-linked manner with typical (but not absolute) early
      gestational male lethality.
    explanation: States the inheritance pattern and the male-lethality qualifier.
biochemical:
- name: Plasma and tissue 8(9)-cholestenol and 8-dehydrocholesterol
  notes: >-
    Elevation of the two sterols immediately upstream of the isomerase block, measurable
    in plasma, in scale from skin lesions, or in cultured lymphoblasts or fibroblasts.
    Sampling lesional skin scale is diagnostically useful precisely because the disorder
    is mosaic: a mildly affected individual may have an unremarkable plasma profile
    while affected clones remain biochemically abnormal.
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      increased concentration of 8(9)-cholestenol and 8-dehydrocholesterol in plasma,
      scales from skin lesions, or cultured lymphoblasts or fibroblasts
    explanation: States the analytes and the acceptable sample types.
diagnosis:
- name: Sterol profiling plus EBP molecular testing
  description: >-
    The diagnosis rests on typical clinical findings together with the characteristic
    sterol elevation and/or an EBP variant. GeneReviews states the criterion separately
    for female probands (mosaic heterozygous variant) and male probands (mosaic
    hemizygous variant), which encodes the point that a male reaching diagnosis is
    expected to be mosaic.
  diagnosis_term:
    preferred_term: diagnostic procedure
    term:
      id: NCIT:C18020
      label: Diagnostic Procedure
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The diagnosis of EBP-CDPX is established in a female proband with typical
      clinical findings; increased concentration of 8(9)-cholestenol and
      8-dehydrocholesterol in plasma, scales from skin lesions, or cultured lymphoblasts
      or fibroblasts; and/or a mosaic heterozygous pathogenic variant in EBP identified
      by molecular genetic testing.
    explanation: States the diagnostic criteria for a female proband.
treatments:
- name: Orthopedic management and surveillance
  description: >-
    Management of kyphoscoliosis, joint problems, linear growth, and leg-length
    discrepancy. No disease-modifying therapy exists; unlike CHILD syndrome, where
    topical cholesterol plus a statin has been shown to clear the skin, no equivalent
    pathogenesis-based intervention has been established for CDPX2.
  therapeutic_modality: BEHAVIORAL
  treatment_term:
    preferred_term: supportive care
    term:
      id: NCIT:C15747
      label: Supportive Care
  target_phenotypes:
  - preferred_term: scoliosis
    term:
      id: HP:0002650
      label: Scoliosis
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Orthopedic evaluations to monitor kyphoscoliosis, joint problems, linear growth,
      and any leg length discrepancy per orthopedist
    explanation: States the recommended orthopedic surveillance.
- name: Emollients and keratolytics for the cutaneous phenotype
  description: Topical management of the ichthyotic scaling, with sun protection.
  therapeutic_modality: OTHER
  treatment_term:
    preferred_term: therapeutic procedure
    term:
      id: NCIT:C49236
      label: Therapeutic Procedure
  target_phenotypes:
  - preferred_term: ichthyosis
    term:
      id: HP:0008064
      label: Ichthyosis
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "dermatologic management with emollients and keratolytics; sun protection"
    explanation: States the recommended dermatologic management.
- name: Cataract extraction
  description: Standard surgical management of the lens opacity.
  therapeutic_modality: SURGERY
  treatment_term:
    preferred_term: surgical procedure
    term:
      id: NCIT:C15329
      label: Surgical Procedure
  target_phenotypes:
  - preferred_term: cataract
    term:
      id: HP:0000518
      label: Cataract
  evidence:
  - reference: PMID:21634086
    reference_title: "EBP-Related X-Linked Chondrodysplasia Punctata."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      cataract extraction and standard treatment of ocular manifestations per
      ophthalmologist
    explanation: States the recommended ophthalmologic management.
animal_models:
- name: Tattered (Td) mouse
  species: Mouse
  genotype: Ebp missense substitution, X-linked semi-dominant (Td), heterozygous females
  publication: PMID:10391218
  description: >-
    A spontaneous X-linked semi-dominant mouse mutation that was independently mapped to
    Ebp, giving a naturally occurring orthologue of CDPX2 in which the mosaic logic of
    the disease is directly visible: heterozygous females develop hairless hyperkeratotic
    patches that read out as coat striping in the adult, and hemizygous males die
    prenatally.
  modeled_mechanisms:
  - target: X-Inactivation Mosaicism
    relationship: RECAPITULATES
    fidelity: HIGH
    description: >-
      The striped coat of the heterozygous female is the mouse equivalent of Blaschko
      lines - a directly observable map of which clones inactivated which X - and
      prenatal death of the hemizygous male reproduces the human male lethality.
    limitations: >-
      Mouse coat striping follows the mouse pattern of clonal skin development, so the
      geometry is not the same as human Blaschko lines even though the underlying
      mosaicism is; and the mouse allele is a single missense substitution, so the model
      speaks to that allele class rather than to nulls.
    readouts:
    - name: Hairless hyperkeratotic skin patches producing adult coat striping
      target: X-Inactivation Mosaicism
      direction: ALTERED
      interpretation: >-
        A visible clonal map of mutant-expressing versus wild-type-expressing skin in the
        heterozygous female.
      evidence:
      - reference: PMID:10391218
        reference_title: "Mutations in a delta 8-delta 7 sterol isomerase in the tattered mouse and X-linked dominant chondrodysplasia punctata. jderry@immunex.com."
        supports: SUPPORT
        evidence_source: MODEL_ORGANISM
        snippet: >-
          Heterozygous females are small and at 4-5 days of age develop patches of
          hyperkeratotic skin where no hair grows, resulting in a striping of the coat in
          adults.
        explanation: Reports the patterned skin phenotype and its progression to coat striping.
    evidence:
    - reference: PMID:10391218
      reference_title: "Mutations in a delta 8-delta 7 sterol isomerase in the tattered mouse and X-linked dominant chondrodysplasia punctata. jderry@immunex.com."
      supports: SUPPORT
      evidence_source: MODEL_ORGANISM
      snippet: "Tattered (Td) is an X-linked, semi-dominant mouse mutation associated with prenatal male lethality."
      explanation: >-
        The X-linked semi-dominant inheritance with prenatal male lethality is the same
        genetic architecture as human CDPX2.
  - target: EBP Sterol-Delta8-Isomerase Deficiency
    relationship: RECAPITULATES
    fidelity: HIGH
    description: >-
      Td is an Ebp allele, so the model carries the same molecular lesion as the human
      disease rather than a phenocopy.
    limitations: >-
      A single amino-acid substitution rather than a null; residual activity is not
      characterized in the cited report.
    evidence:
    - reference: PMID:10391218
      reference_title: "Mutations in a delta 8-delta 7 sterol isomerase in the tattered mouse and X-linked dominant chondrodysplasia punctata. jderry@immunex.com."
      supports: SUPPORT
      evidence_source: MODEL_ORGANISM
      snippet: >-
        We have now identified the defect in Td mice as a single amino acid substitution
        in the delta8-delta7 sterol isomerase emopamil binding protein (Ebp; encoded by
        Ebp in mouse)
      explanation: Establishes that the mouse mutation is in the orthologue of the human disease gene.
differential_diagnoses:
- name: CHILD syndrome
  disease_term:
    preferred_term: CHILD syndrome
    term:
      id: MONDO:0010621
      label: CHILD syndrome
  description: >-
    The nearest mechanistic neighbour: also X-linked, also male-lethal, also a mosaic
    disorder of the post-squalene cholesterol pathway, and also placed in ISDS group 23.
    NSDHL acts one step upstream of EBP. The two are separated clinically by lesion
    geometry rather than by pathway.
  distinguishing_features:
  - CHILD syndrome lesions are strictly lateralized with a sharp body-midline boundary; CDPX2 lesions are bilateral, asymmetric, and follow Blaschko lines
  - CDPX2 carries elevated 8(9)-cholestenol and 8-dehydrocholesterol; NSDHL-related CHILD syndrome carries a methylsterol pattern instead
  - CHILD syndrome has ipsilateral limb reduction defects; CDPX2 has asymmetric rhizomelic shortening without true reduction defects
- name: Rhizomelic chondrodysplasia punctata type 1
  disease_term:
    preferred_term: rhizomelic chondrodysplasia punctata type 1
    term:
      id: MONDO:0008972
      label: rhizomelic chondrodysplasia punctata type 1
  description: >-
    The other major cause of a stippled, rhizomelic newborn skeleton, and the principal
    radiographic differential. It is autosomal recessive and peroxisomal rather than
    X-linked and sterol-pathway, and every cell is uniformly affected.
  distinguishing_features:
  - RCDP1 limb shortening is symmetric; CDPX2 shortening is characteristically asymmetric
  - RCDP1 has profound developmental impairment and seizures; CDPX2 intellect is usually normal
  - RCDP1 is confirmed by erythrocyte plasmalogen deficiency with raised phytanic acid; CDPX2 by the 8(9)-cholestenol / 8-dehydrocholesterol sterol profile
  - RCDP1 affects both sexes equally; at least 95% of live-born CDPX2 patients are female
- name: Brachytelephalangic chondrodysplasia punctata (CDPX1) and its phenocopies
  disease_term:
    preferred_term: X-linked chondrodysplasia punctata 1
    term:
      id: MONDO:0010555
      label: X-linked chondrodysplasia punctata 1
  description: >-
    The X-linked recessive CDP, affecting males, with nasomaxillary hypoplasia and
    distal phalangeal shortening rather than ichthyosis and rhizomelia. Its
    vitamin-K-related phenocopies (maternal warfarin exposure, maternal autoimmune
    disease) share the same radiographic pattern and must be excluded in a stippled
    newborn before either genetic diagnosis is made.
  distinguishing_features:
  - CDPX1 affects males; CDPX2 live births are overwhelmingly female
  - CDPX1 has brachytelephalangy and nasal hypoplasia without ichthyosis, alopecia, or cataract
  - CDPX1 has a normal sterol profile
discussions:
- discussion_id: hypomorphic_male_entity
  kind: KNOWLEDGE_GAP
  status: OPEN
  attaches_to:
  - pathophysiology#Male Hemizygous Lethality
  - genetic#EBP
  prompt: >-
    Is the phenotype of a male hemizygous for a hypomorphic EBP allele a mild variant of
    CDPX2, or a separate X-linked recessive disorder that happens to share the gene?
  rationale: >-
    Two distinct routes let a male with an EBP variant survive, and they predict
    different phenotypes. A mosaic male, or one with a null allele plus a supernumerary
    X, has enzyme-competent clones and presents like a female CDPX2 patient. A male
    hemizygous for a hypomorphic allele has no competent clones anywhere but retains
    partial activity in all of them, and the reported phenotype - digital abnormalities,
    intellectual disability, and short stature, without the ichthyosis and stippling of
    CDPX2 - does not look like CDPX2 at all. Whether that is a nosologically separate
    entity or the low-activity end of one continuum is unsettled, and it matters for
    this entry's boundaries: the ISDS nosology lists only one EBP row, and MONDO carries
    only MONDO:0020603, so a decision either way would change what this entry covers.
    dismech currently treats it as within scope and flags the question here rather than
    creating a second entry on one report.
  evidence:
  - reference: PMID:24700572
    reference_title: "A novel EBP c.224T>A mutation supports the existence of a male-specific disorder independent of CDPX2."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The molecular and biochemical evidence convincingly supports the pathogenicity and
      association of the p.I75N mutation with this newly described phenotype.
    explanation: >-
      The authors argue for a distinct male entity on the strength of one family with
      biochemical confirmation - enough to raise the question, not to settle it.
- discussion_id: sterol_to_stippling_route
  kind: KNOWLEDGE_GAP
  status: OPEN
  attaches_to:
  - pathophysiology#Accumulation of 8(9)-Cholestenol and 8-Dehydrocholesterol
  - pathophysiology#Cellular Cholesterol Deficiency
  prompt: >-
    Which arm of the dual lesion - cholesterol starvation or accumulation of the
    upstream sterols - actually produces the punctate cartilage calcification, and by
    what route?
  rationale: >-
    The enzymology of CDPX2 is settled and the sterol profile is diagnostic, but the
    step from either sterol abnormality to abnormal calcification of cartilage is not.
    The gene-discovery paper put it no more strongly than that the results "suggest a
    role for sterols in bone development", and that remains the state of the argument.
    The question is answerable in principle, because CHILD syndrome supplies a
    precedent: there, a topical trial separating cholesterol replacement from
    suppression of precursor flux showed that neither arm alone accounted for the skin
    phenotype. No equivalent dissection has been done for the CDPX2 skeleton, and the
    two mechanism edges in this entry are annotated as having unknown intermediates for
    that reason.
  evidence:
  - reference: PMID:10391219
    reference_title: "Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hünermann syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Our results indicate that defects in sterol-delta8-isomerase cause CDPX2 and
      suggest a role for sterols in bone development.
    explanation: >-
      The authors' own hedged phrasing marks the gap between the established enzyme
      defect and the skeletal phenotype.
notes: >-
  Curated as a distinct dismech entry rather than folded into a lumped
  chondrodysplasia-punctata entry, because the ISDS 2023 nosology lists it as its own
  row (NOS 23-0020) with its own gene and inheritance, and because the mosaic logic that
  drives its phenotype has no counterpart in the autosomal recessive members of the same
  group. The hypomorphic-allele male phenotype is kept inside this entry and flagged as
  an open question rather than split off, since a single reported family is not a basis
  for a second entry. A member of the Chondrodysplasia_Punctata grouping.
📚

References & Deep Research

References

5
EBP-Related X-Linked Chondrodysplasia Punctata.
No top-level findings curated for this source.
Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hünermann syndrome.
No top-level findings curated for this source.
Mutations in a delta 8-delta 7 sterol isomerase in the tattered mouse and X-linked dominant chondrodysplasia punctata. jderry@immunex.com.
No top-level findings curated for this source.
A novel EBP c.224T>A mutation supports the existence of a male-specific disorder independent of CDPX2.
No top-level findings curated for this source.
Malformation syndromes caused by disorders of cholesterol synthesis.
No top-level findings curated for this source.