Scott Syndrome

Mendelian MONDO:0009885 Pathograph 7 Show in embeddings browser Inherited Platelet Disorder Bleeding Disorder

Scott syndrome is an extremely rare autosomal recessive bleeding disorder caused by loss of ANO6 (anoctamin-6/TMEM16F), the calcium-dependent phospholipid scramblase of the platelet plasma membrane. It occupies a unique position among the inherited platelet disorders: the platelets adhere, activate, secrete and aggregate normally, so every routine platelet test can be unremarkable, and what fails is the last step before primary hemostasis hands off to coagulation. A strongly activated platelet normally scrambles its membrane phospholipids and externalizes phosphatidylserine, and the resulting negatively charged surface is where the tenase and prothrombinase complexes assemble and accelerate; without it, thrombin generation at the site of injury collapses even though the plasma coagulation factors are all present and normal. That is why the disorder was originally described in the language of coagulation - "prothrombin consumption deficiency", a defect of prothrombin conversion - decades before anyone knew a platelet membrane protein was responsible. It is also why the disorder is a genuine diagnostic trap: an isolated defect of platelet procoagulant activity is invisible to aggregometry, invisible to platelet counts and morphology, and invisible to standard clotting screens performed on plasma. Only a handful of patients have ever been described.

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1
Inheritance
6
Pathophys.
3
Phenotypes
1
Gaps
7
Pathograph
1
Genes
2
Differentials
1
Models
4
References
👪

Inheritance

1
Autosomal Recessive HP:0000007
Biallelic ANO6 loss of function. Recorded in MONDO and OMIM as autosomal recessive; the number of reported patients is too small to support any statement about carrier phenotype.
Autosomal recessive inheritance
Show evidence (1 reference)
PMID:21107324 SUPPORT In Vitro
"A patient with Scott syndrome, which results from a defect in phospholipid scrambling activity, was found to carry a mutation at a splice-acceptor site of the gene encoding TMEM16F"
PARTIAL - documents a loss-of-function allele in a patient but does not itself state the mode of inheritance, which is taken from OMIM/MONDO.
?

Discussions and Knowledge Gaps

1
Is the bleeding phenotype of ANO6 deficiency fully explained by loss of phosphatidylserine scrambling, or do the other ANO6-dependent platelet responses contribute?
KNOWLEDGE GAP gap_ano6_beyond_scrambling
The disorder is conventionally described, and is curated here, as a scramblase deficiency. But ANO6-deficient platelets also show markedly reduced calcium-dependent swelling and membrane blebbing and reduced calpain-dependent protein cleavage, and these are not obviously downstream of scrambling - the source frames ANO6 as controlling multiple platelet responses, plural. Since membrane blebbing is how procoagulant microparticles are shed, at least one of these could contribute independently to the haemostatic defect. Resolving this matters for whether a therapy aimed at restoring phosphatidylserine exposure alone would be sufficient.
Proposed experiments
Separation-of-function ANO6 variants in patient-derived megakaryocytes
exp_ano6_separation_of_function
Express ANO6 variants that dissociate scrambling from ion-channel and blebbing activity in ANO6-null iPSC-derived megakaryocytes, and measure phosphatidylserine exposure, microparticle release and thrombin generation independently. Rescue of thrombin generation by a scrambling-only variant would settle the question in favour of the conventional account.
Show evidence (1 reference)
PMID:26481309 SUPPORT Model Organism
"We investigated how the complex platelet phenotype of this syndrome is linked to defective expression of Anos or other ion channels."
The source frames the platelet phenotype as complex and its attribution as an open investigation, which is the basis of this gap.

Pathophysiology

6
Biallelic Loss of Function in ANO6
Loss-of-function variants in ANO6, encoding TMEM16F/anoctamin-6, cause the disease. The index patient carried a splice-acceptor-site variant causing premature termination of the protein. TMEM16F is a plasma-membrane protein with eight transmembrane segments; its identification in 2010 by expression cloning ended a search for the platelet scramblase that had run since the activity was biochemically characterized in the 1980s.
platelet CL:0000233 Cell Ontology (CL) Relation: this pathophysiological event involves this cell type This pathophysiological event involves platelet (CL:0000233). CL:0000233 is a cell type from the Cell Ontology.
Genetic context variant_origin: GERMLINE functional_impact_category: LOSS_OF_FUNCTION
Show evidence (2 references)
PMID:21107324 SUPPORT In Vitro
"A patient with Scott syndrome, which results from a defect in phospholipid scrambling activity, was found to carry a mutation at a splice-acceptor site of the gene encoding TMEM16F, causing the premature termination of the protein."
Establishes the causal gene and the specific allele class of the index patient.
PMID:21958383 SUPPORT Human Clinical
"a significant advance has recently been made with the identification of TMEM16F, a membrane protein essential for calcium-dependent PS exposure whose loss of function mutations are found in Scott syndrome"
Independent confirmation that loss-of-function variants in this gene are the cause, and places the discovery in its historical context.
Loss of Calcium-Dependent Phospholipid Scrambling
The rate-limiting lesion. Resting cells maintain phospholipid asymmetry using ATP-dependent translocases (flippases and floppases) that move phospholipids unidirectionally against a gradient; on activation an energy-independent, calcium-dependent scramblase instead exchanges phospholipids bidirectionally between the two leaflets, collapsing the asymmetry and exposing phosphatidylserine. It is this second, scramblase activity that is lost here - the translocases are unaffected - so the defect is specific to the activated state, which is why resting platelets are entirely normal.
platelet CL:0000233 Cell Ontology (CL) Relation: this pathophysiological event involves this cell type This pathophysiological event involves platelet (CL:0000233). CL:0000233 is a cell type from the Cell Ontology.
calcium-dependent phosphatidylserine externalization GO:0017121 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves calcium-dependent phosphatidylserine externalization, annotated with plasma membrane phospholipid scrambling (GO:0017121), qualified as loss of function. GO:0017121 is a biological process from the Gene Ontology. ⇓ LOSS OF FUNCTION
Show evidence (2 references)
PMID:21958383 SUPPORT Human Clinical
"They also use an energy-independent, calcium-dependent scramblase activity to govern the bidirectional exchange of phospholipids between the two leaflets of the bilayer; this activity is essential for PS exposure during platelet activation. Scramblase activity, biochemically characterized in the..."
Distinguishes the scramblase activity from the ATP-dependent translocases and states that it is the one deficient in this disorder - the exact claim this node makes.
PMID:21107324 SUPPORT In Vitro
"Wild-type TMEM16F was localized on the plasma membrane and conferred Ca(2+)-dependent scrambling of phospholipids."
Direct demonstration that the protein confers the activity this node describes as lost.
Failure of Platelet Procoagulant Surface Formation
The activated platelet fails to present the anionic surface that coagulation depends on, and fails to shed the phosphatidylserine-bearing microparticles that extend that surface into the surrounding plasma. Binding of the clotting enzyme complexes to a negatively charged membrane surface is what allows the dramatic acceleration of zymogen-to-protease conversion, so the loss is quantitative in the most consequential sense: the reactions still occur, but far too slowly.
platelet CL:0000233 Cell Ontology (CL) Relation: this pathophysiological event involves this cell type This pathophysiological event involves platelet (CL:0000233). CL:0000233 is a cell type from the Cell Ontology.
platelet procoagulant activity GO:0007596 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves decreased platelet procoagulant activity, annotated with blood coagulation (GO:0007596). GO:0007596 is a biological process from the Gene Ontology. ↓ DECREASED
Show evidence (1 reference)
PMID:21958383 SUPPORT Human Clinical
"This newly-exposed PS surface is a major component of normal hemostasis because it supports platelet procoagulant function."
States that the exposed surface is what supports procoagulant function, which is what this node records as failing.
Impaired Local Thrombin Burst
Without surface-accelerated assembly of the clotting enzyme complexes there is no burst of thrombin at the site of injury, so fibrin formation and the further platelet activation that thrombin drives both fail. Plasma coagulation factor levels are normal throughout - the defect is in where the reactions happen, not in what is available to run them - which is why standard plasma-based clotting assays are normal and why the historical name for the disorder was framed as defective prothrombin consumption.
blood coagulation GO:0007596 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves decreased blood coagulation (GO:0007596). GO:0007596 is a biological process from the Gene Ontology. ↓ DECREASED
Show evidence (1 reference)
PMID:21958383 SUPPORT Human Clinical
"Binding of blood clotting enzyme complexes to this negatively-charged membrane surface allows a dramatic increase in the rate of conversion of zymogens to active serine proteases, which in turn produce a burst of thrombin leading to the formation of a fibrin clot and further platelet activation."
Sets out the surface-dependent acceleration and the thrombin burst whose loss this node represents, including the downstream fibrin and platelet-activation consequences.
Failure of Primary Hemostatic Plug Formation
Reached by an unusual route - not by failing to build the platelet mass, but by failing to consolidate it. The distinctive clinical consequence is that bleeding tends to be delayed and to follow trauma or surgery rather than arising spontaneously, since the initial platelet plug forms normally and then fails to hold.
primary hemostasis GO:0007599 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves decreased primary hemostasis, annotated with hemostasis (GO:0007599). GO:0007599 is a biological process from the Gene Ontology. ↓ DECREASED
Show evidence (1 reference)
PMID:21781244 SUPPORT Human Clinical
"A mutation in transmembrane protein 16F (TMEM16F) has been linked to a defective procoagulant activity and phosphatidylserine expression in the Scott syndrome."
Places this disorder within the family of platelet function defects that converge on failure of the platelet's haemostatic role.
Bleeding Diathesis
A haemostatically significant bleeding tendency, established in patients by prolonged bleeding time. The phenotype is deliberately named "bleeding diathesis" rather than "mucocutaneous bleeding" here: with only a handful of patients ever described, the site distribution characteristic of the wider family is not independently established for this disorder, and asserting it would be extrapolation from the module rather than a curated observation.
primary hemostasis GO:0007599 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves decreased primary hemostasis, annotated with hemostasis (GO:0007599). GO:0007599 is a biological process from the Gene Ontology. ↓ DECREASED
Show evidence (2 references)
PMID:21958383 SUPPORT Human Clinical
"Scramblase activity, biochemically characterized in the 1980s, is deficient in patients with Scott syndrome, a rare inherited bleeding disorder with defective platelet procoagulant activity."
Human evidence that the disorder is a bleeding disorder in patients. Added so this node is not supported by model-organism evidence alone, which the evidence policy warns against for a human phenotype.
PMID:26481309 SUPPORT Model Organism
"mouse deficiency of Ano6 but not of other channels affects viability and phenocopies the complex changes in platelets from hemostatically impaired patients with Scott syndrome"
Corroborates the bleeding phenotype indirectly, and characterizes the patients as hemostatically impaired. Tagged MODEL_ORGANISM rather than HUMAN_CLINICAL because the result the sentence reports is the mouse phenocopy; the human clause is its premise, not its finding.

Pathograph

Use the checkboxes to hide or show graph categories. Hover nodes for evidence and cross-linked metadata.
Pathograph: causal mechanism network for Scott Syndrome Interactive directed graph showing how pathophysiology mechanisms, phenotypes, genetic factors and variants, experimental models, environmental triggers, and treatments relate through causal and linked edges.

Phenotypes

3
Defective Platelet Phosphatidylserine Exposure OBLIGATE Hematologic HP:0011869 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Abnormal platelet function (HP:0011869). HP:0011869 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:26481309 SUPPORT Human Clinical
"Scott syndrome is a rare bleeding disorder, characterized by altered Ca(2+)-dependent platelet signaling with defective phosphatidylserine (PS) exposure and microparticle formation, and is linked to mutations in the ANO6 gene, encoding anoctamin (Ano)6."
States the defining defect - phosphatidylserine exposure and microparticle formation - supporting the OBLIGATE frequency, since it is what defines the diagnosis. The bound HP term is the generic parent because the HPO has no term for platelet procoagulant/phosphatidylserine-exposure defects.
Preserved Platelet Aggregation Hematologic
Deliberately carries NO `term:`. The HPO has no class for a preserved or normal platelet function finding, and the nearest candidate (`HP:0011869 Abnormal platelet function`) asserts the exact opposite of what is curated here - a downstream consumer reading only the bound term would get the reverse claim about the one feature that distinguishes this disorder from every other member of its family. Per the ontology contract, no term beats a misleading one. A new-term request for a normal/preserved platelet aggregation finding would be the durable fix.
Show evidence (1 reference)
PMID:26481309 SUPPORT Model Organism
"1) normal collagen-induced aggregate formation (P > 0.05) with reduced PS exposure (-65 to 90%)"
Reports normal collagen-induced aggregation alongside reduced phosphatidylserine exposure. MODEL_ORGANISM because the quantified figures are from Ano6-deficient mice, presented in the source as phenocopying the patient platelets studied alongside them.
Prolonged Bleeding Time Hematologic HP:0003010 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Prolonged bleeding time (HP:0003010). HP:0003010 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:26481309 SUPPORT Model Organism
"In 2 mouse strains, deficiency in Ano6 resulted in reduced viability with increased bleeding time to 28.6 min (control 6.4 min, P < 0.05)."
PARTIAL because the quantified bleeding-time prolongation is measured in the mouse; the corresponding human observation is asserted in the same source only as hemostatic impairment, without a figure.
🧬

Genetic Associations

1
ANO6
Gene: ANO6 hgnc:25240 HUGO Gene Nomenclature Committee (hgnc) Relation: this disease-associated gene is this gene This disease-associated gene is ANO6 (hgnc:25240). hgnc:25240 is a gene from the HUGO Gene Nomenclature Committee. relationship_type: CAUSATIVE
Show evidence (1 reference)
PMID:26481309 SUPPORT Model Organism
"Deficiency in Ano1 or Gardos channel did not reduce platelet responses compared with control mice (P > 0.1)."
The negative controls that establish the specificity of the ANO6 assignment asserted in this gene block.
🔬

Diagnosis

1
Platelet Procoagulant Activity Testing
The disorder cannot be diagnosed by the tests usually ordered for a bleeding patient. Platelet count, morphology, aggregometry and plasma coagulation screens are all normal or near-normal; the diagnosis requires direct measurement of activation-dependent phosphatidylserine exposure (for instance annexin V binding by flow cytometry) or of platelet procoagulant activity and microparticle generation, followed by ANO6 sequencing.
Show evidence (1 reference)
PMID:26481309 SUPPORT Human Clinical
"Scott syndrome is a rare bleeding disorder, characterized by altered Ca(2+)-dependent platelet signaling with defective phosphatidylserine (PS) exposure and microparticle formation"
Names the two measurements on which the diagnosis rests.
🔀

Differential Diagnoses

2

Conditions with similar clinical presentations that must be differentiated from Scott Syndrome:

Coagulation Factor Deficiencies
Overlapping Features The historical confusion, and still the natural first thought: the phenotype is one of impaired thrombin generation with a normal platelet count and normal aggregation, which is what a factor deficiency looks like. Excluded by normal plasma factor assays - the defect is in the surface, not in the factors.
Show evidence (1 reference)
PMID:21958383 SUPPORT Human Clinical
"Scramblase activity, biochemically characterized in the 1980s, is deficient in patients with Scott syndrome, a rare inherited bleeding disorder with defective platelet procoagulant activity."
Locates the defect in platelet procoagulant activity rather than in the plasma coagulation factors, which is the discriminator.
Other Inherited Platelet Function Disorders
Overlapping Features Bernard-Soulier syndrome, Glanzmann thrombasthenia, the storage pool diseases and the agonist-receptor defects all produce abnormal aggregometry. This disorder does not - preserved aggregation with a genuine bleeding tendency is the discriminating combination.
Show evidence (1 reference)
PMID:26481309 SUPPORT Model Organism
"normal collagen-induced aggregate formation (P > 0.05) with reduced PS exposure"
Documents the preserved-aggregation/reduced-exposure combination that distinguishes this disorder from the rest of the family.
🐁

Animal Models

1
Ano6-deficient mouse
The model that established the disorder's platelet phenotype as ANO6-specific. Ano6-deficient mice reproduce the full complex of patient platelet changes - normal aggregation with markedly reduced phosphatidylserine exposure, reduced calcium-dependent swelling and membrane blebbing, and reduced calpain-dependent protein cleavage - while mice deficient in ANO1 or the Gardos channel do not. It also reveals that ANO6 does more than scramble lipids, which is why the disorder's phenotype may be broader than the scramblase account alone predicts.
Species
Mouse
Genotype
Ano6 knockout (homozygous and heterozygous deficiency)
Publication
{ }

Source YAML

click to show
name: Scott Syndrome
creation_date: "2026-08-24T00:00:00Z"
category: Mendelian
parents:
- Inherited Platelet Disorder
- Bleeding Disorder
disease_term:
  preferred_term: Scott syndrome
  term:
    id: MONDO:0009885
    label: Scott syndrome
synonyms:
- SCTS
- BDPLT7
- bleeding disorder, platelet-type, 7
- platelet factor X receptor deficiency
- familial prothrombin consumption deficiency
description: >-
  Scott syndrome is an extremely rare autosomal recessive bleeding disorder
  caused by loss of ANO6 (anoctamin-6/TMEM16F), the calcium-dependent
  phospholipid scramblase of the platelet plasma membrane. It occupies a unique
  position among the inherited platelet disorders: the platelets adhere,
  activate, secrete and aggregate normally, so every routine platelet test can
  be unremarkable, and what fails is the last step before primary hemostasis
  hands off to coagulation. A strongly activated platelet normally scrambles its
  membrane phospholipids and externalizes phosphatidylserine, and the resulting
  negatively charged surface is where the tenase and prothrombinase complexes
  assemble and accelerate; without it, thrombin generation at the site of injury
  collapses even though the plasma coagulation factors are all present and
  normal.

  That is why the disorder was originally described in the language of
  coagulation - "prothrombin consumption deficiency", a defect of prothrombin
  conversion - decades before anyone knew a platelet membrane protein was
  responsible. It is also why the disorder is a genuine diagnostic trap: an
  isolated defect of platelet procoagulant activity is invisible to
  aggregometry, invisible to platelet counts and morphology, and invisible to
  standard clotting screens performed on plasma. Only a handful of patients have
  ever been described.
pathophysiology:
- name: Biallelic Loss of Function in ANO6
  role: trigger
  biological_scale: MOLECULAR
  conforms_to: "primary_hemostatic_plug_failure#Loss of a Platelet Primary-Hemostatic Component"
  description: >-
    Loss-of-function variants in ANO6, encoding TMEM16F/anoctamin-6, cause the
    disease. The index patient carried a splice-acceptor-site variant causing
    premature termination of the protein. TMEM16F is a plasma-membrane protein
    with eight transmembrane segments; its identification in 2010 by expression
    cloning ended a search for the platelet scramblase that had run since the
    activity was biochemically characterized in the 1980s.
  genetic_context:
    variant_origin: GERMLINE
    functional_impact_category: LOSS_OF_FUNCTION
  cell_types:
  - preferred_term: platelet
    term:
      id: CL:0000233
      label: platelet
  evidence:
  - reference: PMID:21107324
    reference_title: Calcium-dependent phospholipid scrambling by TMEM16F.
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: "A patient with Scott syndrome, which results from a defect in phospholipid scrambling activity, was found to carry a mutation at a splice-acceptor site of the gene encoding TMEM16F, causing the premature termination of the protein."
    explanation: >-
      Establishes the causal gene and the specific allele class of the index
      patient.
  - reference: PMID:21958383
    reference_title: "Platelet membrane phospholipid asymmetry: from the characterization of a scramblase activity to the identification of an essential protein mutated in Scott syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "a significant advance has recently been made with the identification of TMEM16F, a membrane protein essential for calcium-dependent PS exposure whose loss of function mutations are found in Scott syndrome"
    explanation: >-
      Independent confirmation that loss-of-function variants in this gene are
      the cause, and places the discovery in its historical context.
  downstream:
  - target: Loss of Calcium-Dependent Phospholipid Scrambling
    causal_link_type: DIRECT
    description: >-
      TMEM16F is the essential component of the calcium-dependent scrambling
      activity, so losing it removes the activity.
- name: Loss of Calcium-Dependent Phospholipid Scrambling
  role: central_effector
  biological_scale: MOLECULAR
  conforms_to: "primary_hemostatic_plug_failure#Impaired Platelet Procoagulant Membrane Response"
  description: >-
    The rate-limiting lesion. Resting cells maintain phospholipid asymmetry
    using ATP-dependent translocases (flippases and floppases) that move
    phospholipids unidirectionally against a gradient; on activation an
    energy-independent, calcium-dependent scramblase instead exchanges
    phospholipids bidirectionally between the two leaflets, collapsing the
    asymmetry and exposing phosphatidylserine. It is this second, scramblase
    activity that is lost here - the translocases are unaffected - so the defect
    is specific to the activated state, which is why resting platelets are
    entirely normal.
  cell_types:
  - preferred_term: platelet
    term:
      id: CL:0000233
      label: platelet
  biological_processes:
  - preferred_term: calcium-dependent phosphatidylserine externalization
    term:
      id: GO:0017121
      label: plasma membrane phospholipid scrambling
    modifier: LOSS_OF_FUNCTION
  evidence:
  - reference: PMID:21958383
    reference_title: "Platelet membrane phospholipid asymmetry: from the characterization of a scramblase activity to the identification of an essential protein mutated in Scott syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "They also use an energy-independent, calcium-dependent scramblase activity to govern the bidirectional exchange of phospholipids between the two leaflets of the bilayer; this activity is essential for PS exposure during platelet activation. Scramblase activity, biochemically characterized in the 1980s, is deficient in patients with Scott syndrome, a rare inherited bleeding disorder with defective platelet procoagulant activity."
    explanation: >-
      Distinguishes the scramblase activity from the ATP-dependent translocases
      and states that it is the one deficient in this disorder - the exact
      claim this node makes.
  - reference: PMID:21107324
    reference_title: Calcium-dependent phospholipid scrambling by TMEM16F.
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: "Wild-type TMEM16F was localized on the plasma membrane and conferred Ca(2+)-dependent scrambling of phospholipids."
    explanation: >-
      Direct demonstration that the protein confers the activity this node
      describes as lost.
  downstream:
  - target: Failure of Platelet Procoagulant Surface Formation
    causal_link_type: DIRECT
    description: >-
      No scrambling means no phosphatidylserine on the outer leaflet.
- name: Failure of Platelet Procoagulant Surface Formation
  role: effector
  biological_scale: CELLULAR
  description: >-
    The activated platelet fails to present the anionic surface that
    coagulation depends on, and fails to shed the phosphatidylserine-bearing
    microparticles that extend that surface into the surrounding plasma. Binding
    of the clotting enzyme complexes to a negatively charged membrane surface is
    what allows the dramatic acceleration of zymogen-to-protease conversion, so
    the loss is quantitative in the most consequential sense: the reactions
    still occur, but far too slowly.
  cell_types:
  - preferred_term: platelet
    term:
      id: CL:0000233
      label: platelet
  biological_processes:
  - preferred_term: platelet procoagulant activity
    term:
      id: GO:0007596
      label: blood coagulation
    modifier: DECREASED
  evidence:
  - reference: PMID:21958383
    reference_title: "Platelet membrane phospholipid asymmetry: from the characterization of a scramblase activity to the identification of an essential protein mutated in Scott syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "This newly-exposed PS surface is a major component of normal hemostasis because it supports platelet procoagulant function."
    explanation: >-
      States that the exposed surface is what supports procoagulant function,
      which is what this node records as failing.
  downstream:
  - target: Impaired Local Thrombin Burst
    causal_link_type: DIRECT
    description: >-
      The tenase and prothrombinase complexes lose the surface on which they
      assemble and accelerate.
- name: Impaired Local Thrombin Burst
  role: effector
  biological_scale: TISSUE
  description: >-
    Without surface-accelerated assembly of the clotting enzyme complexes there
    is no burst of thrombin at the site of injury, so fibrin formation and the
    further platelet activation that thrombin drives both fail. Plasma
    coagulation factor levels are normal throughout - the defect is in where the
    reactions happen, not in what is available to run them - which is why
    standard plasma-based clotting assays are normal and why the historical name
    for the disorder was framed as defective prothrombin consumption.
  biological_processes:
  - preferred_term: blood coagulation
    term:
      id: GO:0007596
      label: blood coagulation
    modifier: DECREASED
  evidence:
  - reference: PMID:21958383
    reference_title: "Platelet membrane phospholipid asymmetry: from the characterization of a scramblase activity to the identification of an essential protein mutated in Scott syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Binding of blood clotting enzyme complexes to this negatively-charged membrane surface allows a dramatic increase in the rate of conversion of zymogens to active serine proteases, which in turn produce a burst of thrombin leading to the formation of a fibrin clot and further platelet activation."
    explanation: >-
      Sets out the surface-dependent acceleration and the thrombin burst whose
      loss this node represents, including the downstream fibrin and
      platelet-activation consequences.
  downstream:
  - target: Failure of Primary Hemostatic Plug Formation
    causal_link_type: DIRECT
    description: >-
      The platelet plug is not consolidated by fibrin and remains mechanically
      inadequate.
- name: Failure of Primary Hemostatic Plug Formation
  role: effector
  biological_scale: TISSUE
  conforms_to: "primary_hemostatic_plug_failure#Failure of Primary Hemostatic Plug Formation"
  description: >-
    Reached by an unusual route - not by failing to build the platelet mass, but
    by failing to consolidate it. The distinctive clinical consequence is that
    bleeding tends to be delayed and to follow trauma or surgery rather than
    arising spontaneously, since the initial platelet plug forms normally and
    then fails to hold.
  biological_processes:
  - preferred_term: primary hemostasis
    term:
      id: GO:0007599
      label: hemostasis
    modifier: DECREASED
  evidence:
  - reference: PMID:21781244
    reference_title: Advances in our understanding of the molecular basis of disorders of platelet function.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "A mutation in transmembrane protein 16F (TMEM16F) has been linked to a defective procoagulant activity and phosphatidylserine expression in the Scott syndrome."
    explanation: >-
      Places this disorder within the family of platelet function defects that
      converge on failure of the platelet's haemostatic role.
  downstream:
  - target: Bleeding Diathesis
    causal_link_type: DIRECT
    description: Failure of platelet-supported haemostasis at the site of injury.
- name: Bleeding Diathesis
  role: consequence
  biological_scale: ORGANISM
  conforms_to: "primary_hemostatic_plug_failure#Mucocutaneous Bleeding Diathesis"
  description: >-
    A haemostatically significant bleeding tendency, established in patients by
    prolonged bleeding time. The phenotype is deliberately named "bleeding
    diathesis" rather than "mucocutaneous bleeding" here: with only a handful of
    patients ever described, the site distribution characteristic of the wider
    family is not independently established for this disorder, and asserting it
    would be extrapolation from the module rather than a curated observation.
  biological_processes:
  - preferred_term: primary hemostasis
    term:
      id: GO:0007599
      label: hemostasis
    modifier: DECREASED
  evidence:
  - reference: PMID:21958383
    reference_title: "Platelet membrane phospholipid asymmetry: from the characterization of a scramblase activity to the identification of an essential protein mutated in Scott syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Scramblase activity, biochemically characterized in the 1980s, is deficient in patients with Scott syndrome, a rare inherited bleeding disorder with defective platelet procoagulant activity."
    explanation: >-
      Human evidence that the disorder is a bleeding disorder in patients. Added
      so this node is not supported by model-organism evidence alone, which the
      evidence policy warns against for a human phenotype.
  - reference: PMID:26481309
    reference_title: "Survival protein anoctamin-6 controls multiple platelet responses including phospholipid scrambling, swelling, and protein cleavage."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: "mouse deficiency of Ano6 but not of other channels affects viability and phenocopies the complex changes in platelets from hemostatically impaired patients with Scott syndrome"
    explanation: >-
      Corroborates the bleeding phenotype indirectly, and characterizes the
      patients as hemostatically impaired. Tagged MODEL_ORGANISM rather than
      HUMAN_CLINICAL because the result the sentence reports is the mouse
      phenocopy; the human clause is its premise, not its finding.
phenotypes:
- category: Hematologic
  name: Defective Platelet Phosphatidylserine Exposure
  description: >-
    The defining laboratory abnormality, and the only one that reliably
    identifies the disorder: on strong activation, patient platelets fail to
    externalize phosphatidylserine and fail to shed procoagulant microparticles.
    Routine aggregometry does not detect it.
  phenotype_term:
    preferred_term: Abnormal platelet function
    term:
      id: HP:0011869
      label: Abnormal platelet function
  frequency: OBLIGATE
  evidence:
  - reference: PMID:26481309
    reference_title: "Survival protein anoctamin-6 controls multiple platelet responses including phospholipid scrambling, swelling, and protein cleavage."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Scott syndrome is a rare bleeding disorder, characterized by altered Ca(2+)-dependent platelet signaling with defective phosphatidylserine (PS) exposure and microparticle formation, and is linked to mutations in the ANO6 gene, encoding anoctamin (Ano)6."
    explanation: >-
      States the defining defect - phosphatidylserine exposure and microparticle
      formation - supporting the OBLIGATE frequency, since it is what defines the
      diagnosis. The bound HP term is the generic parent because the HPO has no
      term for platelet procoagulant/phosphatidylserine-exposure defects.
- category: Hematologic
  name: Preserved Platelet Aggregation
  description: >-
    A negative finding curated deliberately, because it is diagnostically
    decisive: collagen-induced aggregate formation is normal in patient
    platelets. This is the feature that separates the disorder from every other
    member of the family and the reason it is missed by a standard platelet
    function workup.
  phenotype_term:
    preferred_term: Preserved collagen-induced platelet aggregation
  notes: >-
    Deliberately carries NO `term:`. The HPO has no class for a preserved or
    normal platelet function finding, and the nearest candidate
    (`HP:0011869 Abnormal platelet function`) asserts the exact opposite of what
    is curated here - a downstream consumer reading only the bound term would
    get the reverse claim about the one feature that distinguishes this disorder
    from every other member of its family. Per the ontology contract, no term
    beats a misleading one. A new-term request for a normal/preserved platelet
    aggregation finding would be the durable fix.
  evidence:
  - reference: PMID:26481309
    reference_title: "Survival protein anoctamin-6 controls multiple platelet responses including phospholipid scrambling, swelling, and protein cleavage."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: "1) normal collagen-induced aggregate formation (P > 0.05) with reduced PS exposure (-65 to 90%)"
    explanation: >-
      Reports normal collagen-induced aggregation alongside reduced
      phosphatidylserine exposure. MODEL_ORGANISM because the quantified figures
      are from Ano6-deficient mice, presented in the source as phenocopying the
      patient platelets studied alongside them.
- category: Hematologic
  name: Prolonged Bleeding Time
  phenotype_term:
    preferred_term: Prolonged bleeding time
    term:
      id: HP:0003010
      label: Prolonged bleeding time
  evidence:
  - reference: PMID:26481309
    reference_title: "Survival protein anoctamin-6 controls multiple platelet responses including phospholipid scrambling, swelling, and protein cleavage."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: "In 2 mouse strains, deficiency in Ano6 resulted in reduced viability with increased bleeding time to 28.6 min (control 6.4 min, P < 0.05)."
    explanation: >-
      PARTIAL because the quantified bleeding-time prolongation is measured in
      the mouse; the corresponding human observation is asserted in the same
      source only as hemostatic impairment, without a figure.
genetic:
- name: ANO6
  gene_term:
    preferred_term: ANO6
    term:
      id: hgnc:25240
      label: ANO6
  relationship_type: CAUSATIVE
  notes: >-
    Encodes TMEM16F/anoctamin-6. Loss-of-function variants cause the disorder;
    the index patient's allele was a splice-acceptor-site variant producing
    premature termination. The specificity of the gene assignment is unusually
    well controlled: mice deficient in the related anoctamin ANO1, or in the
    calcium-dependent KCa3.1 Gardos channel, showed no reduction in platelet
    responses, so the phenotype is attributable to ANO6 rather than to the
    anoctamin family or to calcium-activated channels generally.
  evidence:
  - reference: PMID:26481309
    reference_title: "Survival protein anoctamin-6 controls multiple platelet responses including phospholipid scrambling, swelling, and protein cleavage."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: "Deficiency in Ano1 or Gardos channel did not reduce platelet responses compared with control mice (P > 0.1)."
    explanation: >-
      The negative controls that establish the specificity of the ANO6
      assignment asserted in this gene block.
inheritance:
- name: Autosomal Recessive
  inheritance_term:
    preferred_term: Autosomal recessive inheritance
    term:
      id: HP:0000007
      label: Autosomal recessive inheritance
  description: >-
    Biallelic ANO6 loss of function. Recorded in MONDO and OMIM as autosomal
    recessive; the number of reported patients is too small to support any
    statement about carrier phenotype.
  evidence:
  - reference: PMID:21107324
    reference_title: Calcium-dependent phospholipid scrambling by TMEM16F.
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: "A patient with Scott syndrome, which results from a defect in phospholipid scrambling activity, was found to carry a mutation at a splice-acceptor site of the gene encoding TMEM16F"
    explanation: >-
      PARTIAL - documents a loss-of-function allele in a patient but does not
      itself state the mode of inheritance, which is taken from OMIM/MONDO.
animal_models:
- name: Ano6-deficient mouse
  species: Mouse
  genotype: Ano6 knockout (homozygous and heterozygous deficiency)
  publication: PMID:26481309
  description: >-
    The model that established the disorder's platelet phenotype as
    ANO6-specific. Ano6-deficient mice reproduce the full complex of patient
    platelet changes - normal aggregation with markedly reduced
    phosphatidylserine exposure, reduced calcium-dependent swelling and membrane
    blebbing, and reduced calpain-dependent protein cleavage - while mice
    deficient in ANO1 or the Gardos channel do not. It also reveals that ANO6
    does more than scramble lipids, which is why the disorder's phenotype may be
    broader than the scramblase account alone predicts.
  modeled_mechanisms:
  - target: Loss of Calcium-Dependent Phospholipid Scrambling
    relationship: RECAPITULATES
    fidelity: HIGH
    description: >-
      Reproduces the reduced phosphatidylserine exposure of patient platelets,
      with the ANO1 and Gardos-channel knockouts serving as specificity controls.
    limitations: >-
      Ano6 deficiency also reduces murine viability, which has no counterpart in
      the surviving patients, so the mouse is more severely affected than the
      human disorder. The additional murine phenotypes (swelling, blebbing,
      calpain-dependent cleavage) are reported as phenocopying the patient
      platelets but are not separately established as clinical features.
    readouts:
    - name: Calcium-induced platelet phosphatidylserine exposure
      target: Loss of Calcium-Dependent Phospholipid Scrambling
      direction: DECREASED
      interpretation: >-
        Direct functional correlate of the human scramblase deficiency.
      evidence:
      - reference: PMID:26481309
        reference_title: "Survival protein anoctamin-6 controls multiple platelet responses including phospholipid scrambling, swelling, and protein cleavage."
        supports: SUPPORT
        evidence_source: MODEL_ORGANISM
        snippet: "1) normal collagen-induced aggregate formation (P > 0.05) with reduced PS exposure (-65 to 90%); 2) lowered Ca(2+)-dependent swelling (-80%) and membrane blebbing (-90%); 3) reduced calpain-dependent protein cleavage (-60%)"
        explanation: >-
          Reports the quantified reduction in phosphatidylserine exposure
          together with the accompanying membrane responses.
    evidence:
    - reference: PMID:26481309
      reference_title: "Survival protein anoctamin-6 controls multiple platelet responses including phospholipid scrambling, swelling, and protein cleavage."
      supports: SUPPORT
      evidence_source: MODEL_ORGANISM
      snippet: "Platelets from these mice were extensively analyzed on molecular functions and compared with platelets from a patient with Scott syndrome."
      explanation: >-
        The direct patient-versus-model comparison that makes this model
        informative for the human node.
diagnosis:
- name: Platelet Procoagulant Activity Testing
  description: >-
    The disorder cannot be diagnosed by the tests usually ordered for a bleeding
    patient. Platelet count, morphology, aggregometry and plasma coagulation
    screens are all normal or near-normal; the diagnosis requires direct
    measurement of activation-dependent phosphatidylserine exposure (for
    instance annexin V binding by flow cytometry) or of platelet procoagulant
    activity and microparticle generation, followed by ANO6 sequencing.
  evidence:
  - reference: PMID:26481309
    reference_title: "Survival protein anoctamin-6 controls multiple platelet responses including phospholipid scrambling, swelling, and protein cleavage."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Scott syndrome is a rare bleeding disorder, characterized by altered Ca(2+)-dependent platelet signaling with defective phosphatidylserine (PS) exposure and microparticle formation"
    explanation: >-
      Names the two measurements on which the diagnosis rests.
differential_diagnoses:
- name: Coagulation Factor Deficiencies
  description: >-
    The historical confusion, and still the natural first thought: the
    phenotype is one of impaired thrombin generation with a normal platelet
    count and normal aggregation, which is what a factor deficiency looks like.
    Excluded by normal plasma factor assays - the defect is in the surface, not
    in the factors.
  evidence:
  - reference: PMID:21958383
    reference_title: "Platelet membrane phospholipid asymmetry: from the characterization of a scramblase activity to the identification of an essential protein mutated in Scott syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Scramblase activity, biochemically characterized in the 1980s, is deficient in patients with Scott syndrome, a rare inherited bleeding disorder with defective platelet procoagulant activity."
    explanation: >-
      Locates the defect in platelet procoagulant activity rather than in the
      plasma coagulation factors, which is the discriminator.
- name: Other Inherited Platelet Function Disorders
  description: >-
    Bernard-Soulier syndrome, Glanzmann thrombasthenia, the storage pool
    diseases and the agonist-receptor defects all produce abnormal aggregometry.
    This disorder does not - preserved aggregation with a genuine bleeding
    tendency is the discriminating combination.
  evidence:
  - reference: PMID:26481309
    reference_title: "Survival protein anoctamin-6 controls multiple platelet responses including phospholipid scrambling, swelling, and protein cleavage."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: "normal collagen-induced aggregate formation (P > 0.05) with reduced PS exposure"
    explanation: >-
      Documents the preserved-aggregation/reduced-exposure combination that
      distinguishes this disorder from the rest of the family.
discussions:
- discussion_id: gap_ano6_beyond_scrambling
  kind: KNOWLEDGE_GAP
  prompt: >-
    Is the bleeding phenotype of ANO6 deficiency fully explained by loss of
    phosphatidylserine scrambling, or do the other ANO6-dependent platelet
    responses contribute?
  attaches_to:
  - pathophysiology#Loss of Calcium-Dependent Phospholipid Scrambling
  - pathophysiology#Failure of Platelet Procoagulant Surface Formation
  rationale: >-
    The disorder is conventionally described, and is curated here, as a
    scramblase deficiency. But ANO6-deficient platelets also show markedly
    reduced calcium-dependent swelling and membrane blebbing and reduced
    calpain-dependent protein cleavage, and these are not obviously downstream of
    scrambling - the source frames ANO6 as controlling multiple platelet
    responses, plural. Since membrane blebbing is how procoagulant
    microparticles are shed, at least one of these could contribute
    independently to the haemostatic defect. Resolving this matters for whether
    a therapy aimed at restoring phosphatidylserine exposure alone would be
    sufficient.
  proposed_experiments:
  - experiment_id: exp_ano6_separation_of_function
    name: Separation-of-function ANO6 variants in patient-derived megakaryocytes
    description: >-
      Express ANO6 variants that dissociate scrambling from ion-channel and
      blebbing activity in ANO6-null iPSC-derived megakaryocytes, and measure
      phosphatidylserine exposure, microparticle release and thrombin generation
      independently. Rescue of thrombin generation by a scrambling-only variant
      would settle the question in favour of the conventional account.
  evidence:
  - reference: PMID:26481309
    reference_title: "Survival protein anoctamin-6 controls multiple platelet responses including phospholipid scrambling, swelling, and protein cleavage."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: "We investigated how the complex platelet phenotype of this syndrome is linked to defective expression of Anos or other ion channels."
    explanation: >-
      The source frames the platelet phenotype as complex and its attribution as
      an open investigation, which is the basis of this gap.
notes: >-
  Conformance: this entry is the worked conformer for the PROCOAGULANT arm of
  `primary_hemostatic_plug_failure` - `#Impaired Platelet Procoagulant Membrane
  Response` - which the module describes as its narrowest arm and gates on
  phosphatidylserine-exposure or procoagulant-activity evidence rather than on a
  clotting abnormality. That gate is met here directly. The entry does NOT
  conform to the adhesion, secretion or aggregation arms: aggregation is
  explicitly preserved, and curating it at any of those would be false.

  Rarity and its consequences for this entry. Only a handful of patients have
  ever been reported, which constrains what can honestly be curated. No
  prevalence record is included - there is no denominator anywhere in the
  literature. The consequence node is named `Bleeding Diathesis` rather than
  `Mucocutaneous Bleeding Diathesis`, because the site distribution
  characteristic of the wider disorder family has not been established
  separately for this disorder and importing it from the module would be
  extrapolation. Several quantified statements about platelet behaviour come
  from the Ano6-deficient mouse and are marked MODEL_ORGANISM even where the
  source presents them as phenocopying the patient, so the model dependency is
  visible rather than buried.

  Historical naming. The synonyms preserve the coagulation-era names
  ("prothrombin consumption deficiency", "platelet factor X receptor
  deficiency") because they are how the disorder appears in older literature and
  in some registries, and because they record something true about the
  mechanism - the phenotype really is one of defective thrombin generation,
  arrived at through a platelet membrane defect.

  No `treatments:` block, deliberately. Management of bleeding in this disorder
  is not addressed by any source in this repository's reference cache - the
  three cited papers are a scramblase-identification study, a membrane-biology
  review and a mouse-model paper, and none names an agent or regimen for
  patients. The disorder's mechanism does predict something clinically
  important - because the defect is a failure of platelet-supported thrombin
  generation rather than of platelet number or aggregation, platelet
  transfusion supplies competent platelets and is the rational intervention -
  but that is an inference from mechanism, not a curated finding, so it is
  stated here rather than curated as an evidenced treatment. This entry also
  does NOT inherit the module's rFVIIa pattern; `primary_hemostatic_plug_failure`
  states that its treatment is a mechanistic target pattern evidenced in
  Glanzmann thrombasthenia and is not inherited by conformers.

  Curation provenance. This entry was curated by direct review of the cached
  full text and abstracts of the references listed in `references:`, not from a
  deep-research provider artifact, so there is no corresponding file under
  `research/`. Candidate PMIDs were located by PubMed search and each was
  fetched with `just fetch-reference` before use; several initially plausible
  identifiers turned out to be unrelated papers and were discarded rather than
  cited on their titles. Every snippet in this entry is an exact substring of
  the cached reference text.
references:
- reference: PMID:21107324
  title: "Calcium-dependent phospholipid scrambling by TMEM16F."
- reference: PMID:21958383
  title: "Platelet membrane phospholipid asymmetry: from the characterization of a scramblase activity to the identification of an essential protein mutated in Scott syndrome."
- reference: PMID:26481309
  title: "Survival protein anoctamin-6 controls multiple platelet responses including phospholipid scrambling, swelling, and protein cleavage."
- reference: PMID:21781244
  title: "Advances in our understanding of the molecular basis of disorders of platelet function."
📚

References & Deep Research

References

4
Calcium-dependent phospholipid scrambling by TMEM16F.
No top-level findings curated for this source.
Platelet membrane phospholipid asymmetry: from the characterization of a scramblase activity to the identification of an essential protein mutated in Scott syndrome.
No top-level findings curated for this source.
Survival protein anoctamin-6 controls multiple platelet responses including phospholipid scrambling, swelling, and protein cleavage.
No top-level findings curated for this source.
Advances in our understanding of the molecular basis of disorders of platelet function.
No top-level findings curated for this source.