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2
Mappings
1
Inheritance
6
Pathophys.
9
Phenotypes
14
Pathograph
3
Genes
2
Medical Actions
3
Subtypes
4
Differentials
🔗

Mappings

MONDO
MONDO:0015776 rhizomelic chondrodysplasia punctata
skos:broadMatch MONDO
This entry covers the plasmalogen-synthesis single-enzyme forms (RCDP2/3/4) under the clinical RCDP umbrella; the umbrella also subsumes the import-receptor forms RCDP1 (PEX7) and RCDP5 (PEX5), which are curated separately, so a broadMatch is used rather than exactMatch.
MONDO:0017986 disorder of plasmalogens biosynthesis Not Yet Curated
skos:broadMatch MONDO
The three convergent enzyme defects are disorders of plasmalogen biosynthesis; this MONDO grouping additionally includes import-receptor RCDP forms.
👪

Inheritance

1
Autosomal recessive inheritance HP:0000007
RCDP2 (GNPAT), RCDP3 (AGPS), and RCDP4 (FAR1 deficiency) each require biallelic pathogenic variants and are inherited as autosomal recessive traits.
Autosomal recessive inheritance
Show evidence (1 reference)
"GNPAT | HGNC:4416 | glyceronephosphate O-acyltransferase deficiency | MONDO:0100273 | AR | Definitive"
ClinGen records autosomal recessive inheritance (AR) for GNPAT-related RCDP2; AGPS and FAR1-deficiency assertions are likewise AR.

Subtypes

3
RCDP type 2 (GNPAT / DHAPAT deficiency) MONDO:0009112
GNPAT hgnc:4416
Autosomal recessive deficiency of glyceronephosphate O-acyltransferase (GNPAT/DHAPAT), which catalyses the first committed peroxisomal step of ether-lipid synthesis. RCDP2 patients have an isolated DHAPAT deficiency with no other peroxisomal abnormalities, producing plasmalogen deficiency with a classic RCDP clinical picture.
Show evidence (2 references)
"GNPAT | HGNC:4416 | glyceronephosphate O-acyltransferase deficiency | MONDO:0100273 | AR | Definitive"
ClinGen classifies the GNPAT-RCDP2 (glyceronephosphate O-acyltransferase deficiency) gene-disease relationship as Definitive with autosomal recessive inheritance.
PMID:9536089 SUPPORT Human Clinical
"All RCDP type 2 patients analyzed were found to contain mutations in their DHAPAT cDNA. This demonstrates that RCDP type 2 is the result of mutations in DHAPAT."
Identifies DHAPAT (GNPAT) mutations as the molecular cause of RCDP type 2.
RCDP type 3 (AGPS / alkyl-DHAP synthase deficiency) MONDO:0010823
AGPS hgnc:327
Autosomal recessive deficiency of alkylglycerone-phosphate synthase (AGPS/alkyl-DHAP synthase), the peroxisomal enzyme that forms the characteristic ether bond of plasmalogens. RCDP3 is an isolated enzyme deficiency causing plasmalogen deficiency with the RCDP clinical phenotype.
Show evidence (2 references)
"AGPS | HGNC:327 | alkylglycerone-phosphate synthase deficiency | MONDO:0100274 | AR | Definitive"
ClinGen classifies the AGPS-RCDP3 (alkylglycerone-phosphate synthase deficiency) gene-disease relationship as Definitive with autosomal recessive inheritance.
PMID:9553082 SUPPORT In Vitro
"A patient with an isolated deficiency in alkyl-dihydroxyacetonephosphate (DHAP) synthase activity had normal levels of this protein. Analysis at the cDNA level revealed a missense mutation leading to a R419H substitution in the enzyme of this patient."
Identifies an AGPS (alkyl-DHAP synthase) point mutation as the cause of an isolated single-enzyme deficiency (RCDP3).
RCDP type 4 (FAR1 deficiency) MONDO:0014510
FAR1 hgnc:26222
Autosomal recessive deficiency of fatty acyl-CoA reductase 1 (FAR1), the rate-limiting enzyme that supplies the fatty alcohol for ether-lipid synthesis. Loss-of-function abolishes reductase activity and depletes plasmalogens. The clinical phenotype emphasises severe intellectual disability, early-onset epilepsy, microcephaly, congenital cataracts, growth retardation, and spasticity, frequently without the rhizomelia seen in RCDP1-3.
Show evidence (2 references)
"FAR1 | HGNC:26222 | fatty acyl-CoA reductase 1 deficiency | MONDO:0014510 | AR | Moderate"
ClinGen classifies the FAR1-deficiency (RCDP4) gene-disease relationship as Moderate with autosomal recessive inheritance.
PMID:25439727 SUPPORT Human Clinical
"We thus expand the spectrum of clinical features associated with defects in plasmalogen biosynthesis to include FAR1 deficiency as a cause of syndromic severe intellectual disability with cataracts, epilepsy, and growth retardation but without rhizomelia."
Establishes FAR1 deficiency (RCDP4) as a plasmalogen-biosynthesis disorder with a distinctive cataract/epilepsy phenotype often without rhizomelia.

Pathophysiology

6
Plasmalogen-Synthesis Enzyme Deficiency
Loss-of-function of a single ether-lipid biosynthetic enzyme — FAR1 (rate-limiting fatty-alcohol supply), GNPAT/DHAPAT (first acyltransferase step), or AGPS/alkyl-DHAP synthase (ether-bond formation) — blocks plasmalogen synthesis at a discrete step. Unlike RCDP1, the peroxisomal PTS2 import machinery is intact, so only the affected enzyme is lost and other PTS2-dependent functions (notably phytanic acid alpha-oxidation by phytanoyl-CoA hydroxylase) are preserved.
FAR1 hgnc:26222 GNPAT hgnc:4416 AGPS hgnc:327
ether lipid biosynthetic process GO:0008611 ↓ DECREASED
peroxisome GO:0005777
Show evidence (2 references)
PMID:9536089 SUPPORT Human Clinical
"Patients in the two other subgroups have been reported to be either deficient in the activity of DHAPAT (RCDP type 2) or alkyl-DHAP synthase (RCDP type 3) while no other abnormalities could be observed."
Documents that RCDP2 and RCDP3 are isolated single-enzyme deficiencies with no other peroxisomal abnormalities, distinguishing them from the generalized PTS2-import lesion of RCDP1.
PMID:25439727 SUPPORT In Vitro
"all three mutations abolished the reductase activity of FAR1, given that no fatty alcohols could be detected."
Functional assays show FAR1 loss-of-function abolishes reductase activity, the enzymatic lesion upstream of plasmalogen deficiency in RCDP4.
Plasmalogen (Ether Lipid) Deficiency
Plasmalogens are ether glycerophospholipids with a vinyl-ether bond at the sn-1 position. Their depletion disrupts membrane and myelin composition and developmental signaling in bone, brain, lens, and other tissues, accounting for the skeletal, ocular, and neurologic manifestations shared with RCDP1.
ether lipid biosynthetic process GO:0008611 ↓ DECREASED
Show evidence (1 reference)
PMID:22627108 SUPPORT Human Clinical
"Their biosynthesis starts in peroxisomes, and defects at these steps cause the malformation syndrome, Rhizomelic Chondrodysplasia Punctata (RCDP)."
Links defects in peroxisomal plasmalogen biosynthesis to the RCDP malformation syndrome.
Preserved Phytanic Acid Alpha-Oxidation
Because the lesion is a single ether-lipid enzyme downstream of peroxisomal matrix import, the PTS2 enzyme phytanoyl-CoA hydroxylase is still imported and functional, so phytanic acid alpha-oxidation is preserved and plasma phytanic acid is normal. This is the key biochemical fork distinguishing the single-enzyme forms (RCDP2/3/4) from the import-receptor forms RCDP1 (PEX7) and RCDP5 (PEX5), in which loss of the PTS2 receptor knocks out both plasmalogen synthesis and phytanic alpha-oxidation.
fatty acid alpha-oxidation GO:0001561
Show evidence (1 reference)
PMID:9536089 SUPPORT Human Clinical
"Patients in the two other subgroups have been reported to be either deficient in the activity of DHAPAT (RCDP type 2) or alkyl-DHAP synthase (RCDP type 3) while no other abnormalities could be observed."
The isolated single-enzyme deficiency with no other peroxisomal abnormalities implies preserved phytanic alpha-oxidation, unlike the generalized RCDP1 import lesion.
Skeletal Dysplasia
Plasmalogen-dependent disruption of cartilage and bone development produces rhizomelic (proximal) limb shortening, chondrodysplasia punctata with stippled epiphyses, and coronal vertebral clefts in RCDP2/3 (and variably in RCDP4).
chondrocyte CL:0000138
Show evidence (1 reference)
PMID:25439727 SUPPORT Human Clinical
"Rhizomelic chondrodysplasia punctata (RCDP) is a group of disorders with overlapping clinical features including rhizomelia, chondrodysplasia punctata, coronal clefts, cervical dysplasia, congenital cataracts, profound postnatal growth retardation, severe intellectual disability, and seizures."
Enumerates the rhizomelia, chondrodysplasia punctata, and coronal clefts that define the skeletal dysplasia across RCDP forms.
Congenital Cataract
Plasmalogen deficiency disrupts lens fiber development, producing cataracts usually present at birth or in early infancy; cataract is a consistent feature across all three single-enzyme forms.
lens fiber cell CL:0011004
Show evidence (1 reference)
PMID:25439727 SUPPORT Human Clinical
"we report the identification of mutations in another gene in plasmalogen biosynthesis, fatty acyl-CoA reductase 1 (FAR1), in two families affected by severe intellectual disability, early-onset epilepsy, microcephaly, congenital cataracts, growth retardation, and spasticity."
Documents congenital cataracts as a core feature of FAR1 deficiency (RCDP4) and the broader plasmalogen-synthesis group.
Neurological Impairment
CNS plasmalogen deficiency impairs neuronal and myelin development, producing severe intellectual disability and seizures; FAR1 deficiency additionally features microcephaly and spasticity.
neuron CL:0000540 oligodendrocyte CL:0000128
Show evidence (1 reference)
PMID:25439727 SUPPORT Human Clinical
"in two families affected by severe intellectual disability, early-onset epilepsy, microcephaly, congenital cataracts, growth retardation, and spasticity."
Documents the severe neurodevelopmental phenotype (intellectual disability, epilepsy, microcephaly, spasticity) of FAR1 deficiency.

Pathograph

Use the checkboxes to hide or show graph categories. Hover nodes for evidence and cross-linked metadata.
Pathograph: causal mechanism network for Rhizomelic Chondrodysplasia Punctata, Plasmalogen-Synthesis Defect Interactive directed graph showing how pathophysiology mechanisms, phenotypes, genetic factors and variants, experimental models, environmental triggers, and treatments relate through causal and linked edges.

Phenotypes

9
Eye 1
Cataract Cataract HP:0000518
Onset: CONGENITAL
Show evidence (1 reference)
PMID:25439727 SUPPORT Human Clinical
"severe intellectual disability, early-onset epilepsy, microcephaly, congenital cataracts, growth retardation, and spasticity."
Documents congenital cataracts in FAR1 deficiency and the RCDP group.
Head and Neck 1
Microcephaly Microcephaly HP:0000252
Show evidence (1 reference)
PMID:25439727 SUPPORT Human Clinical
"severe intellectual disability, early-onset epilepsy, microcephaly, congenital cataracts"
Documents microcephaly as a feature of FAR1 deficiency.
Musculoskeletal 2
Coronal Cleft Vertebrae Coronal cleft vertebrae HP:0003417
Onset: CONGENITAL
Show evidence (1 reference)
PMID:25439727 SUPPORT Human Clinical
"overlapping clinical features including rhizomelia, chondrodysplasia punctata, coronal clefts, cervical dysplasia, congenital cataracts"
Lists coronal clefts among the overlapping skeletal features of RCDP.
Spasticity Spasticity HP:0001257
Show evidence (1 reference)
PMID:25439727 SUPPORT Human Clinical
"congenital cataracts, growth retardation, and spasticity."
Documents spasticity as a feature of FAR1 deficiency.
Nervous System 2
Intellectual Disability Intellectual disability HP:0001249
Show evidence (1 reference)
PMID:25439727 SUPPORT Human Clinical
"two families affected by severe intellectual disability, early-onset epilepsy, microcephaly, congenital cataracts, growth retardation, and spasticity."
Documents severe intellectual disability in FAR1 deficiency and the RCDP group.
Seizures Seizure HP:0001250
Show evidence (1 reference)
PMID:25439727 SUPPORT Human Clinical
"severe intellectual disability, early-onset epilepsy, microcephaly"
Documents early-onset epilepsy/seizures in FAR1 deficiency.
Growth 2
Rhizomelia Rhizomelia HP:0008905
Onset: CONGENITAL
Show evidence (1 reference)
PMID:25439727 SUPPORT Human Clinical
"Rhizomelic chondrodysplasia punctata (RCDP) is a group of disorders with overlapping clinical features including rhizomelia, chondrodysplasia punctata, coronal clefts, cervical dysplasia, congenital cataracts, profound postnatal growth retardation, severe intellectual disability, and seizures."
Lists rhizomelia among the overlapping clinical features of the RCDP group.
Postnatal Growth Deficiency Postnatal growth retardation HP:0008897
Show evidence (1 reference)
PMID:25439727 SUPPORT Human Clinical
"profound postnatal growth retardation, severe intellectual disability, and seizures."
Documents profound postnatal growth retardation in the RCDP group.
Other 1
Chondrodysplasia Punctata Epiphyseal stippling HP:0010655
Onset: CONGENITAL
Show evidence (1 reference)
PMID:25439727 SUPPORT Human Clinical
"overlapping clinical features including rhizomelia, chondrodysplasia punctata, coronal clefts"
Documents chondrodysplasia punctata as a defining feature of the RCDP group.
🧬

Genetic Associations

3
GNPAT (Biallelic loss-of-function variants in GNPAT (encoding DHAPAT) cause RCDP type 2, an isolated DHAPAT deficiency. ClinGen classifies the gene-disease relationship as Definitive.)
Gene: GNPAT hgnc:4416
Show evidence (1 reference)
PMID:9536089 SUPPORT Human Clinical
"All RCDP type 2 patients analyzed were found to contain mutations in their DHAPAT cDNA. This demonstrates that RCDP type 2 is the result of mutations in DHAPAT."
Establishes DHAPAT (GNPAT) mutations as the cause of RCDP type 2.
AGPS (Biallelic loss-of-function variants in AGPS (encoding alkyl-DHAP synthase) cause RCDP type 3, an isolated alkyl-DHAP synthase deficiency. ClinGen classifies the gene-disease relationship as Definitive.)
Gene: AGPS hgnc:327
Show evidence (1 reference)
PMID:9553082 SUPPORT In Vitro
"Expression of a recombinant protein carrying this mutation in Escherichia coli yielded an inactive enzyme"
Functional expression confirms the AGPS missense mutation abolishes enzyme activity, establishing causality for RCDP3.
FAR1 (Biallelic loss-of-function variants in FAR1 cause RCDP type 4 (FAR1 deficiency) by abolishing the rate-limiting fatty acyl-CoA reductase step of plasmalogen synthesis. ClinGen classifies the gene-disease relationship as Moderate.)
Gene: FAR1 hgnc:26222
Show evidence (1 reference)
PMID:25439727 SUPPORT In Vitro
"all three mutations abolished the reductase activity of FAR1, given that no fatty alcohols could be detected."
Functional assays establish that the FAR1 variants abolish reductase activity, the molecular cause of RCDP4.
💊

Medical Actions

2
Supportive and Rehabilitative Care
Action: supportive care MAXO:0000950
Management is supportive: physical therapy and orthopedic procedures for contractures and skeletal deformity, antiseizure medication for epilepsy, and developmental support.
Target Phenotypes: Seizure HP:0001250 Spasticity HP:0001257
Show evidence (1 reference)
PMID:22627108 NO_EVIDENCE Human Clinical
"Their biosynthesis starts in peroxisomes, and defects at these steps cause the malformation syndrome, Rhizomelic Chondrodysplasia Punctata (RCDP)."
No disease-modifying therapy exists for RCDP; management is supportive care by clinical convention. This snippet describes the disease mechanism, not a treatment intervention.
Cataract Extraction
Action: surgical procedure MAXO:0000004
Surgical removal of congenital cataracts can restore and/or preserve vision.
Target Phenotypes: Cataract HP:0000518
Show evidence (1 reference)
PMID:25439727 NO_EVIDENCE Human Clinical
"severe intellectual disability, early-onset epilepsy, microcephaly, congenital cataracts, growth retardation, and spasticity."
Congenital cataracts are present in RCDP and are amenable to surgical extraction by standard ophthalmologic practice; this snippet establishes cataract as a feature but does not describe the surgical intervention.
🔬

Biochemical Markers

2
Plasmalogens (Decreased)
Context: Erythrocyte and tissue plasmalogens are reduced because a plasmalogen- synthesis enzyme (FAR1, GNPAT, or AGPS) is non-functional. Reduced plasmalogens are the shared biochemical readout of the convergence.
Pathograph Readouts
Readout Of Plasmalogen (Ether Lipid) Deficiency Negative Diagnostic
Decreased plasmalogens report failure of ether-lipid synthesis at the affected enzyme step.
Show evidence (1 reference)
PMID:25439727 SUPPORT Human Clinical
"We also observed reduced plasmalogens in red blood cells in one individual to a range similar to that seen in individuals with RCDP, further supporting abolished FAR1 activity."
Reduced red-blood-cell plasmalogens are the biochemical hallmark of the single-enzyme RCDP forms.
Phytanic acid (Normal)
Context: Plasma phytanic acid is characteristically NORMAL in the single-enzyme plasmalogen-synthesis forms because phytanoyl-CoA hydroxylase (a PTS2 enzyme) is still imported and functional. Normal phytanic acid distinguishes RCDP2/3/4 from RCDP1 (PEX7) and RCDP5 (PEX5), where phytanic accumulates.
Show evidence (1 reference)
PMID:9536089 SUPPORT Human Clinical
"Patients in the two other subgroups have been reported to be either deficient in the activity of DHAPAT (RCDP type 2) or alkyl-DHAP synthase (RCDP type 3) while no other abnormalities could be observed."
The isolated enzyme deficiency with no other peroxisomal abnormalities implies preserved phytanic alpha-oxidation and normal phytanic acid.
🔀

Differential Diagnoses

4

Conditions with similar clinical presentations that must be differentiated from Rhizomelic Chondrodysplasia Punctata, Plasmalogen-Synthesis Defect:

Overlapping Features RCDP1 is caused by biallelic PEX7 variants that abolish the cytosolic PTS2 import receptor, producing a generalized loss of PTS2 cargo — including both plasmalogen-synthesis enzymes and phytanoyl-CoA hydroxylase — so plasmalogens are deficient AND phytanic acid accumulates.
Distinguishing Features
  • RCDP1 is a PTS2 import-receptor defect with combined plasmalogen deficiency and elevated phytanic acid; RCDP2/3/4 are single-enzyme plasmalogen-synthesis defects with normal phytanic acid.
Rhizomelic Chondrodysplasia Punctata Type 5 Not Yet Curated MONDO:0014743
Overlapping Features RCDP5 results from PEX5 variants affecting the PEX7-binding domain, which impairs PTS2 import similarly to RCDP1 (an import-receptor lesion), not a single ether-lipid enzyme defect.
Distinguishing Features
  • RCDP5 is a PEX5 PTS2-binding-domain import defect; the single-enzyme forms in this entry leave peroxisomal import intact.
FAR1 Upregulation Disorder (gain-of-function) Not Yet Curated MONDO:0100230
Overlapping Features Autosomal dominant gain-of-function FAR1 variants disrupt the feedback control of FAR1 stability, causing plasmalogen ELEVATION rather than deficiency. Despite the opposite biochemical direction, affected individuals share a neurodevelopmental phenotype with cataracts and spasticity, illustrating the tight homeostatic requirement for plasmalogen dosage.
Distinguishing Features
  • FAR1 gain-of-function (AD) causes plasmalogen elevation via impaired FAR1 feedback regulation; RCDP4 is FAR1 loss-of-function (AR) causing plasmalogen deficiency.
Overlapping Features ZSD results from generalized peroxisomal matrix-import failure (PEX genes) affecting both PTS1 and PTS2 cargo, producing VLCFA accumulation alongside plasmalogen and phytanic/bile-acid abnormalities.
Distinguishing Features
  • ZSD has generalized peroxisomal dysfunction with elevated VLCFA; the single-enzyme RCDP forms have an isolated plasmalogen-synthesis defect with normal VLCFA and phytanic acid.
{ }

Source YAML

click to show
name: Rhizomelic Chondrodysplasia Punctata, Plasmalogen-Synthesis Defect
creation_date: "2026-06-12T00:00:00Z"
category: Mendelian
description: >-
  Rhizomelic chondrodysplasia punctata (RCDP) types 2-4 are autosomal recessive
  inborn errors of ether-phospholipid (plasmalogen) biosynthesis caused by
  biallelic loss-of-function variants in a single plasmalogen-synthesis enzyme
  rather than in a peroxisome import receptor. Three sequential enzymes feed the
  pathway: fatty acyl-CoA reductase 1 (FAR1; rate-limiting, supplies the fatty
  alcohol), glyceronephosphate O-acyltransferase (GNPAT/DHAPAT), and
  alkylglycerone-phosphate synthase (AGPS/alkyl-DHAP synthase; forms the
  ether bond). Loss of any one of these enzymes converges on the same lesion —
  cellular plasmalogen deficiency — producing a phenotype that overlaps
  classic RCDP1: rhizomelic limb shortening, chondrodysplasia punctata
  (stippled epiphyses), coronal vertebral clefts, congenital cataracts, severe
  growth and intellectual impairment, and seizures (FAR1 deficiency is
  distinguished by prominent microcephaly, epilepsy, and spasticity, often
  without rhizomelia). The critical biochemical discriminator from RCDP1 is
  that phytanic acid alpha-oxidation is preserved: RCDP1 (PEX7) and the PEX5
  PTS2-binding-domain form (RCDP5) lose the PTS2 import receptor and therefore
  knock out both plasmalogen synthesis and phytanoyl-CoA hydroxylase, whereas
  these single-enzyme defects lie downstream of import and leave phytanic
  alpha-oxidation intact, so phytanic acid is normal. This entry models the
  single-enzyme plasmalogen-synthesis convergence (RCDP2 GNPAT, RCDP3 AGPS,
  RCDP4 FAR1 deficiency); the import-receptor forms RCDP1 (PEX7) and RCDP5
  (PEX5), and the mechanistically inverse FAR1 gain-of-function disorder
  (plasmalogen elevation), are captured as differentials.
disease_term:
  preferred_term: rhizomelic chondrodysplasia punctata (plasmalogen-synthesis defect)
  term:
    id: MONDO:0015776
    label: rhizomelic chondrodysplasia punctata
synonyms:
- RCDP types 2-4
- Plasmalogen-biosynthesis-defect rhizomelic chondrodysplasia punctata
- GNPAT-, AGPS-, and FAR1-related rhizomelic chondrodysplasia punctata
- Single-enzyme RCDP
mappings:
  mondo_mappings:
  - term:
      id: MONDO:0015776
      label: rhizomelic chondrodysplasia punctata
    mapping_predicate: skos:broadMatch
    mapping_source: MONDO
    mapping_justification: >-
      This entry covers the plasmalogen-synthesis single-enzyme forms (RCDP2/3/4)
      under the clinical RCDP umbrella; the umbrella also subsumes the
      import-receptor forms RCDP1 (PEX7) and RCDP5 (PEX5), which are curated
      separately, so a broadMatch is used rather than exactMatch.
  - term:
      id: MONDO:0017986
      label: disorder of plasmalogens biosynthesis
    mapping_predicate: skos:broadMatch
    mapping_source: MONDO
    mapping_justification: >-
      The three convergent enzyme defects are disorders of plasmalogen
      biosynthesis; this MONDO grouping additionally includes import-receptor
      RCDP forms.
parents:
- peroxisomal single enzyme/protein defect
- disorder of plasmalogens biosynthesis
- inborn errors of metabolism
has_subtypes:
- name: RCDP2
  display_name: RCDP type 2 (GNPAT / DHAPAT deficiency)
  subtype_term:
    preferred_term: rhizomelic chondrodysplasia punctata type 2
    term:
      id: MONDO:0009112
      label: rhizomelic chondrodysplasia punctata type 2
  description: >-
    Autosomal recessive deficiency of glyceronephosphate O-acyltransferase
    (GNPAT/DHAPAT), which catalyses the first committed peroxisomal step of
    ether-lipid synthesis. RCDP2 patients have an isolated DHAPAT deficiency
    with no other peroxisomal abnormalities, producing plasmalogen deficiency
    with a classic RCDP clinical picture.
  genes:
  - preferred_term: GNPAT
    term:
      id: hgnc:4416
      label: GNPAT
  evidence:
  - reference: CGGV:assertion_ba96f922-5ec9-4404-b2e4-66c31c2dcda6-2020-02-07T170000.000Z
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "GNPAT | HGNC:4416 | glyceronephosphate O-acyltransferase deficiency | MONDO:0100273 | AR | Definitive"
    explanation: >-
      ClinGen classifies the GNPAT-RCDP2 (glyceronephosphate O-acyltransferase
      deficiency) gene-disease relationship as Definitive with autosomal
      recessive inheritance.
  - reference: PMID:9536089
    reference_title: "Acyl-CoA:dihydroxyacetonephosphate acyltransferase: cloning of the human cDNA and resolution of the molecular basis in rhizomelic chondrodysplasia punctata type 2."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      All RCDP type 2 patients analyzed were found to contain mutations in their
      DHAPAT cDNA. This demonstrates that RCDP type 2 is the result of mutations
      in DHAPAT.
    explanation: >-
      Identifies DHAPAT (GNPAT) mutations as the molecular cause of RCDP type 2.
- name: RCDP3
  display_name: RCDP type 3 (AGPS / alkyl-DHAP synthase deficiency)
  subtype_term:
    preferred_term: rhizomelic chondrodysplasia punctata type 3
    term:
      id: MONDO:0010823
      label: rhizomelic chondrodysplasia punctata type 3
  description: >-
    Autosomal recessive deficiency of alkylglycerone-phosphate synthase
    (AGPS/alkyl-DHAP synthase), the peroxisomal enzyme that forms the
    characteristic ether bond of plasmalogens. RCDP3 is an isolated enzyme
    deficiency causing plasmalogen deficiency with the RCDP clinical phenotype.
  genes:
  - preferred_term: AGPS
    term:
      id: hgnc:327
      label: AGPS
  evidence:
  - reference: CGGV:assertion_aba640e2-c95d-414b-8c72-d22769e4366a-2022-04-22T160000.000Z
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "AGPS | HGNC:327 | alkylglycerone-phosphate synthase deficiency | MONDO:0100274 | AR | Definitive"
    explanation: >-
      ClinGen classifies the AGPS-RCDP3 (alkylglycerone-phosphate synthase
      deficiency) gene-disease relationship as Definitive with autosomal
      recessive inheritance.
  - reference: PMID:9553082
    reference_title: Alkyl-dihydroxyacetonephosphate synthase. Fate in peroxisome biogenesis disorders and identification of the point mutation underlying a single enzyme deficiency.
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      A patient with an isolated deficiency in alkyl-dihydroxyacetonephosphate
      (DHAP) synthase activity had normal levels of this protein. Analysis at
      the cDNA level revealed a missense mutation leading to a R419H
      substitution in the enzyme of this patient.
    explanation: >-
      Identifies an AGPS (alkyl-DHAP synthase) point mutation as the cause of an
      isolated single-enzyme deficiency (RCDP3).
- name: RCDP4
  display_name: RCDP type 4 (FAR1 deficiency)
  subtype_term:
    preferred_term: fatty acyl-CoA reductase 1 deficiency
    term:
      id: MONDO:0014510
      label: fatty acyl-CoA reductase 1 deficiency
  description: >-
    Autosomal recessive deficiency of fatty acyl-CoA reductase 1 (FAR1), the
    rate-limiting enzyme that supplies the fatty alcohol for ether-lipid
    synthesis. Loss-of-function abolishes reductase activity and depletes
    plasmalogens. The clinical phenotype emphasises severe intellectual
    disability, early-onset epilepsy, microcephaly, congenital cataracts, growth
    retardation, and spasticity, frequently without the rhizomelia seen in
    RCDP1-3.
  genes:
  - preferred_term: FAR1
    term:
      id: hgnc:26222
      label: FAR1
  evidence:
  - reference: CGGV:assertion_91f6f864-5d07-4891-94b5-7f73bd71752c-2025-07-08T160000.000Z
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "FAR1 | HGNC:26222 | fatty acyl-CoA reductase 1 deficiency | MONDO:0014510 | AR | Moderate"
    explanation: >-
      ClinGen classifies the FAR1-deficiency (RCDP4) gene-disease relationship
      as Moderate with autosomal recessive inheritance.
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      We thus expand the spectrum of clinical features associated with defects
      in plasmalogen biosynthesis to include FAR1 deficiency as a cause of
      syndromic severe intellectual disability with cataracts, epilepsy, and
      growth retardation but without rhizomelia.
    explanation: >-
      Establishes FAR1 deficiency (RCDP4) as a plasmalogen-biosynthesis disorder
      with a distinctive cataract/epilepsy phenotype often without rhizomelia.
inheritance:
- name: Autosomal recessive inheritance
  inheritance_term:
    preferred_term: Autosomal recessive inheritance
    term:
      id: HP:0000007
      label: Autosomal recessive inheritance
  description: >-
    RCDP2 (GNPAT), RCDP3 (AGPS), and RCDP4 (FAR1 deficiency) each require
    biallelic pathogenic variants and are inherited as autosomal recessive
    traits.
  evidence:
  - reference: CGGV:assertion_ba96f922-5ec9-4404-b2e4-66c31c2dcda6-2020-02-07T170000.000Z
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "GNPAT | HGNC:4416 | glyceronephosphate O-acyltransferase deficiency | MONDO:0100273 | AR | Definitive"
    explanation: >-
      ClinGen records autosomal recessive inheritance (AR) for GNPAT-related
      RCDP2; AGPS and FAR1-deficiency assertions are likewise AR.
pathophysiology:
- name: Plasmalogen-Synthesis Enzyme Deficiency
  description: >-
    Loss-of-function of a single ether-lipid biosynthetic enzyme — FAR1
    (rate-limiting fatty-alcohol supply), GNPAT/DHAPAT (first acyltransferase
    step), or AGPS/alkyl-DHAP synthase (ether-bond formation) — blocks
    plasmalogen synthesis at a discrete step. Unlike RCDP1, the peroxisomal
    PTS2 import machinery is intact, so only the affected enzyme is lost and
    other PTS2-dependent functions (notably phytanic acid alpha-oxidation by
    phytanoyl-CoA hydroxylase) are preserved.
  genes:
  - preferred_term: FAR1
    term:
      id: hgnc:26222
      label: FAR1
  - preferred_term: GNPAT
    term:
      id: hgnc:4416
      label: GNPAT
  - preferred_term: AGPS
    term:
      id: hgnc:327
      label: AGPS
  biological_processes:
  - preferred_term: ether lipid biosynthetic process
    term:
      id: GO:0008611
      label: ether lipid biosynthetic process
    modifier: DECREASED
  cellular_components:
  - preferred_term: peroxisome
    term:
      id: GO:0005777
      label: peroxisome
  evidence:
  - reference: PMID:9536089
    reference_title: "Acyl-CoA:dihydroxyacetonephosphate acyltransferase: cloning of the human cDNA and resolution of the molecular basis in rhizomelic chondrodysplasia punctata type 2."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Patients in the two other subgroups have been reported to be either
      deficient in the activity of DHAPAT (RCDP type 2) or alkyl-DHAP synthase
      (RCDP type 3) while no other abnormalities could be observed.
    explanation: >-
      Documents that RCDP2 and RCDP3 are isolated single-enzyme deficiencies
      with no other peroxisomal abnormalities, distinguishing them from the
      generalized PTS2-import lesion of RCDP1.
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      all three mutations abolished the reductase activity of FAR1, given that
      no fatty alcohols could be detected.
    explanation: >-
      Functional assays show FAR1 loss-of-function abolishes reductase activity,
      the enzymatic lesion upstream of plasmalogen deficiency in RCDP4.
  downstream:
  - target: Plasmalogen (Ether Lipid) Deficiency
    description: >-
      Loss of any single step in the FAR1 -> GNPAT -> AGPS sequence blocks
      ether-phospholipid synthesis and depletes cellular plasmalogens.
    causal_link_type: DIRECT
    evidence:
    - reference: PMID:25439727
      reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        We also observed reduced plasmalogens in red blood cells in one
        individual to a range similar to that seen in individuals with RCDP,
        further supporting abolished FAR1 activity.
      explanation: >-
        Demonstrates that a single-enzyme (FAR1) defect reduces plasmalogens to
        the RCDP range, linking the enzyme lesion to plasmalogen deficiency.
- name: Plasmalogen (Ether Lipid) Deficiency
  description: >-
    Plasmalogens are ether glycerophospholipids with a vinyl-ether bond at the
    sn-1 position. Their depletion disrupts membrane and myelin composition and
    developmental signaling in bone, brain, lens, and other tissues, accounting
    for the skeletal, ocular, and neurologic manifestations shared with RCDP1.
  biological_processes:
  - preferred_term: ether lipid biosynthetic process
    term:
      id: GO:0008611
      label: ether lipid biosynthetic process
    modifier: DECREASED
  chemical_entities:
  - preferred_term: plasmalogens
    term:
      id: CHEBI:64611
      label: ether lipid
    modifier: DECREASED
  evidence:
  - reference: PMID:22627108
    reference_title: Functions of plasmalogen lipids in health and disease.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Their biosynthesis starts in peroxisomes, and defects at these steps cause
      the malformation syndrome, Rhizomelic Chondrodysplasia Punctata (RCDP).
    explanation: >-
      Links defects in peroxisomal plasmalogen biosynthesis to the RCDP
      malformation syndrome.
  downstream:
  - target: Skeletal Dysplasia
    description: >-
      Plasmalogen deficiency disrupts cartilage and bone development, producing
      rhizomelic shortening and chondrodysplasia punctata.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:22627108
      reference_title: Functions of plasmalogen lipids in health and disease.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        The RCDP phenotype predicts developmental roles for plasmalogens in
        bone, brain, lens, lung, kidney and heart.
      explanation: >-
        Supports a plasmalogen-dependent developmental role in bone underlying
        the skeletal dysplasia.
  - target: Congenital Cataract
    description: >-
      Plasmalogen deficiency disrupts lens fiber development, producing
      congenital cataracts.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:22627108
      reference_title: Functions of plasmalogen lipids in health and disease.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        The RCDP phenotype predicts developmental roles for plasmalogens in
        bone, brain, lens, lung, kidney and heart.
      explanation: >-
        Supports a plasmalogen-dependent developmental role in the lens
        underlying cataract.
  - target: Neurological Impairment
    description: >-
      Plasmalogen deficiency disrupts CNS neuronal and myelin development,
      producing intellectual disability, epilepsy, and (in FAR1 deficiency)
      microcephaly and spasticity.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:36720320
      reference_title: Regulation of plasmalogen biosynthesis in mammalian cells and tissues.
      supports: SUPPORT
      evidence_source: MODEL_ORGANISM
      snippet: >-
        Dysregulation of plasmalogen synthesis impairs cholesterol synthesis in
        cells and brain, resulting in the reduced expression of genes such as
        mRNA encoding myelin basic protein, a phenotype found in the cerebellum
        of plasmalogen-deficient mice.
      explanation: >-
        Plasmalogen-deficient mice show impaired brain cholesterol synthesis and
        reduced myelin basic protein expression, a mechanistic link between
        plasmalogen deficiency and the CNS/myelin phenotype.
- name: Preserved Phytanic Acid Alpha-Oxidation
  description: >-
    Because the lesion is a single ether-lipid enzyme downstream of peroxisomal
    matrix import, the PTS2 enzyme phytanoyl-CoA hydroxylase is still imported
    and functional, so phytanic acid alpha-oxidation is preserved and plasma
    phytanic acid is normal. This is the key biochemical fork distinguishing the
    single-enzyme forms (RCDP2/3/4) from the import-receptor forms RCDP1 (PEX7)
    and RCDP5 (PEX5), in which loss of the PTS2 receptor knocks out both
    plasmalogen synthesis and phytanic alpha-oxidation.
  biological_processes:
  - preferred_term: fatty acid alpha-oxidation
    term:
      id: GO:0001561
      label: fatty acid alpha-oxidation
  chemical_entities:
  - preferred_term: phytanic acid
    term:
      id: CHEBI:16285
      label: phytanic acid
  evidence:
  - reference: PMID:9536089
    reference_title: "Acyl-CoA:dihydroxyacetonephosphate acyltransferase: cloning of the human cDNA and resolution of the molecular basis in rhizomelic chondrodysplasia punctata type 2."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Patients in the two other subgroups have been reported to be either
      deficient in the activity of DHAPAT (RCDP type 2) or alkyl-DHAP synthase
      (RCDP type 3) while no other abnormalities could be observed.
    explanation: >-
      The isolated single-enzyme deficiency with no other peroxisomal
      abnormalities implies preserved phytanic alpha-oxidation, unlike the
      generalized RCDP1 import lesion.
- name: Skeletal Dysplasia
  description: >-
    Plasmalogen-dependent disruption of cartilage and bone development produces
    rhizomelic (proximal) limb shortening, chondrodysplasia punctata with
    stippled epiphyses, and coronal vertebral clefts in RCDP2/3 (and variably
    in RCDP4).
  cell_types:
  - preferred_term: chondrocyte
    term:
      id: CL:0000138
      label: chondrocyte
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Rhizomelic chondrodysplasia punctata (RCDP) is a group of disorders with
      overlapping clinical features including rhizomelia, chondrodysplasia
      punctata, coronal clefts, cervical dysplasia, congenital cataracts,
      profound postnatal growth retardation, severe intellectual disability, and
      seizures.
    explanation: >-
      Enumerates the rhizomelia, chondrodysplasia punctata, and coronal clefts
      that define the skeletal dysplasia across RCDP forms.
- name: Congenital Cataract
  description: >-
    Plasmalogen deficiency disrupts lens fiber development, producing cataracts
    usually present at birth or in early infancy; cataract is a consistent
    feature across all three single-enzyme forms.
  cell_types:
  - preferred_term: lens fiber cell
    term:
      id: CL:0011004
      label: lens fiber cell
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      we report the identification of mutations in another gene in plasmalogen
      biosynthesis, fatty acyl-CoA reductase 1 (FAR1), in two families affected
      by severe intellectual disability, early-onset epilepsy, microcephaly,
      congenital cataracts, growth retardation, and spasticity.
    explanation: >-
      Documents congenital cataracts as a core feature of FAR1 deficiency
      (RCDP4) and the broader plasmalogen-synthesis group.
- name: Neurological Impairment
  description: >-
    CNS plasmalogen deficiency impairs neuronal and myelin development,
    producing severe intellectual disability and seizures; FAR1 deficiency
    additionally features microcephaly and spasticity.
  cell_types:
  - preferred_term: neuron
    term:
      id: CL:0000540
      label: neuron
  - preferred_term: oligodendrocyte
    term:
      id: CL:0000128
      label: oligodendrocyte
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      in two families affected by severe intellectual disability, early-onset
      epilepsy, microcephaly, congenital cataracts, growth retardation, and
      spasticity.
    explanation: >-
      Documents the severe neurodevelopmental phenotype (intellectual
      disability, epilepsy, microcephaly, spasticity) of FAR1 deficiency.
phenotypes:
- name: Rhizomelia
  category: Musculoskeletal
  diagnostic: true
  description: >-
    Symmetrical proximal limb shortening is a defining skeletal feature of
    RCDP2/3; it is often absent in FAR1 deficiency (RCDP4).
  phenotype_term:
    preferred_term: Rhizomelia
    term:
      id: HP:0008905
      label: Rhizomelia
    onset:
      onset_category: CONGENITAL
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Rhizomelic chondrodysplasia punctata (RCDP) is a group of disorders with
      overlapping clinical features including rhizomelia, chondrodysplasia
      punctata, coronal clefts, cervical dysplasia, congenital cataracts,
      profound postnatal growth retardation, severe intellectual disability, and
      seizures.
    explanation: >-
      Lists rhizomelia among the overlapping clinical features of the RCDP group.
- name: Chondrodysplasia Punctata
  category: Musculoskeletal
  diagnostic: true
  description: >-
    Punctate calcifications in cartilage with epiphyseal stippling are the
    radiographic hallmark of the disorder.
  phenotype_term:
    preferred_term: Epiphyseal stippling
    term:
      id: HP:0010655
      label: Epiphyseal stippling
    onset:
      onset_category: CONGENITAL
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      overlapping clinical features including rhizomelia, chondrodysplasia
      punctata, coronal clefts
    explanation: >-
      Documents chondrodysplasia punctata as a defining feature of the RCDP
      group.
- name: Coronal Cleft Vertebrae
  category: Musculoskeletal
  description: >-
    Coronal clefts of the vertebral bodies are a characteristic spinal
    radiographic finding in the RCDP group.
  phenotype_term:
    preferred_term: Coronal cleft vertebrae
    term:
      id: HP:0003417
      label: Coronal cleft vertebrae
    onset:
      onset_category: CONGENITAL
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      overlapping clinical features including rhizomelia, chondrodysplasia
      punctata, coronal clefts, cervical dysplasia, congenital cataracts
    explanation: >-
      Lists coronal clefts among the overlapping skeletal features of RCDP.
- name: Cataract
  category: Visual
  diagnostic: true
  description: >-
    Congenital cataracts occur across all three single-enzyme forms and are a
    prominent feature of FAR1 deficiency.
  phenotype_term:
    preferred_term: Cataract
    term:
      id: HP:0000518
      label: Cataract
    onset:
      onset_category: CONGENITAL
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      severe intellectual disability, early-onset epilepsy, microcephaly,
      congenital cataracts, growth retardation, and spasticity.
    explanation: >-
      Documents congenital cataracts in FAR1 deficiency and the RCDP group.
- name: Postnatal Growth Deficiency
  category: Growth
  description: >-
    Profound postnatal growth retardation is characteristic of the RCDP group.
  phenotype_term:
    preferred_term: Postnatal growth retardation
    term:
      id: HP:0008897
      label: Postnatal growth retardation
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      profound postnatal growth retardation, severe intellectual disability, and
      seizures.
    explanation: >-
      Documents profound postnatal growth retardation in the RCDP group.
- name: Intellectual Disability
  category: Neurologic
  description: >-
    Severe intellectual disability is consistent across the single-enzyme RCDP
    forms.
  phenotype_term:
    preferred_term: Intellectual disability
    term:
      id: HP:0001249
      label: Intellectual disability
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      two families affected by severe intellectual disability, early-onset
      epilepsy, microcephaly, congenital cataracts, growth retardation, and
      spasticity.
    explanation: >-
      Documents severe intellectual disability in FAR1 deficiency and the RCDP
      group.
- name: Seizures
  category: Neurologic
  description: >-
    Seizures/early-onset epilepsy occur in the RCDP group and are prominent in
    FAR1 deficiency.
  phenotype_term:
    preferred_term: Seizure
    term:
      id: HP:0001250
      label: Seizure
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      severe intellectual disability, early-onset epilepsy, microcephaly
    explanation: >-
      Documents early-onset epilepsy/seizures in FAR1 deficiency.
- name: Microcephaly
  category: Neurologic
  description: >-
    Microcephaly is a distinguishing feature of FAR1 deficiency (RCDP4).
  phenotype_term:
    preferred_term: Microcephaly
    term:
      id: HP:0000252
      label: Microcephaly
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      severe intellectual disability, early-onset epilepsy, microcephaly,
      congenital cataracts
    explanation: >-
      Documents microcephaly as a feature of FAR1 deficiency.
- name: Spasticity
  category: Neurologic
  description: >-
    Spasticity is a distinguishing feature of FAR1 deficiency (RCDP4).
  phenotype_term:
    preferred_term: Spasticity
    term:
      id: HP:0001257
      label: Spasticity
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      congenital cataracts, growth retardation, and spasticity.
    explanation: >-
      Documents spasticity as a feature of FAR1 deficiency.
genetic:
- name: GNPAT
  gene_term:
    preferred_term: GNPAT
    term:
      id: hgnc:4416
      label: GNPAT
  association: >-
    Biallelic loss-of-function variants in GNPAT (encoding DHAPAT) cause RCDP
    type 2, an isolated DHAPAT deficiency. ClinGen classifies the gene-disease
    relationship as Definitive.
  evidence:
  - reference: PMID:9536089
    reference_title: "Acyl-CoA:dihydroxyacetonephosphate acyltransferase: cloning of the human cDNA and resolution of the molecular basis in rhizomelic chondrodysplasia punctata type 2."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      All RCDP type 2 patients analyzed were found to contain mutations in their
      DHAPAT cDNA. This demonstrates that RCDP type 2 is the result of mutations
      in DHAPAT.
    explanation: >-
      Establishes DHAPAT (GNPAT) mutations as the cause of RCDP type 2.
- name: AGPS
  gene_term:
    preferred_term: AGPS
    term:
      id: hgnc:327
      label: AGPS
  association: >-
    Biallelic loss-of-function variants in AGPS (encoding alkyl-DHAP synthase)
    cause RCDP type 3, an isolated alkyl-DHAP synthase deficiency. ClinGen
    classifies the gene-disease relationship as Definitive.
  evidence:
  - reference: PMID:9553082
    reference_title: Alkyl-dihydroxyacetonephosphate synthase. Fate in peroxisome biogenesis disorders and identification of the point mutation underlying a single enzyme deficiency.
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      Expression of a recombinant protein carrying this mutation in Escherichia
      coli yielded an inactive enzyme
    explanation: >-
      Functional expression confirms the AGPS missense mutation abolishes enzyme
      activity, establishing causality for RCDP3.
- name: FAR1
  gene_term:
    preferred_term: FAR1
    term:
      id: hgnc:26222
      label: FAR1
  association: >-
    Biallelic loss-of-function variants in FAR1 cause RCDP type 4 (FAR1
    deficiency) by abolishing the rate-limiting fatty acyl-CoA reductase step of
    plasmalogen synthesis. ClinGen classifies the gene-disease relationship as
    Moderate.
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      all three mutations abolished the reductase activity of FAR1, given that
      no fatty alcohols could be detected.
    explanation: >-
      Functional assays establish that the FAR1 variants abolish reductase
      activity, the molecular cause of RCDP4.
biochemical:
- name: Plasmalogens
  presence: Decreased
  context: >-
    Erythrocyte and tissue plasmalogens are reduced because a plasmalogen-
    synthesis enzyme (FAR1, GNPAT, or AGPS) is non-functional. Reduced
    plasmalogens are the shared biochemical readout of the convergence.
  readouts:
  - target: Plasmalogen (Ether Lipid) Deficiency
    relationship: READOUT_OF
    direction: NEGATIVE
    endpoint_context: DIAGNOSTIC
    interpretation: >-
      Decreased plasmalogens report failure of ether-lipid synthesis at the
      affected enzyme step.
  biomarker_term:
    preferred_term: plasmalogens
    term:
      id: CHEBI:64611
      label: ether lipid
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      We also observed reduced plasmalogens in red blood cells in one individual
      to a range similar to that seen in individuals with RCDP, further
      supporting abolished FAR1 activity.
    explanation: >-
      Reduced red-blood-cell plasmalogens are the biochemical hallmark of the
      single-enzyme RCDP forms.
- name: Phytanic acid
  presence: Normal
  context: >-
    Plasma phytanic acid is characteristically NORMAL in the single-enzyme
    plasmalogen-synthesis forms because phytanoyl-CoA hydroxylase (a PTS2 enzyme)
    is still imported and functional. Normal phytanic acid distinguishes
    RCDP2/3/4 from RCDP1 (PEX7) and RCDP5 (PEX5), where phytanic accumulates.
  biomarker_term:
    preferred_term: phytanic acid
    term:
      id: CHEBI:16285
      label: phytanic acid
  evidence:
  - reference: PMID:9536089
    reference_title: "Acyl-CoA:dihydroxyacetonephosphate acyltransferase: cloning of the human cDNA and resolution of the molecular basis in rhizomelic chondrodysplasia punctata type 2."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Patients in the two other subgroups have been reported to be either
      deficient in the activity of DHAPAT (RCDP type 2) or alkyl-DHAP synthase
      (RCDP type 3) while no other abnormalities could be observed.
    explanation: >-
      The isolated enzyme deficiency with no other peroxisomal abnormalities
      implies preserved phytanic alpha-oxidation and normal phytanic acid.
treatments:
- name: Supportive and Rehabilitative Care
  description: >-
    Management is supportive: physical therapy and orthopedic procedures for
    contractures and skeletal deformity, antiseizure medication for epilepsy,
    and developmental support.
  treatment_term:
    preferred_term: supportive care
    term:
      id: MAXO:0000950
      label: supportive care
  target_phenotypes:
  - preferred_term: Seizure
    term:
      id: HP:0001250
      label: Seizure
  - preferred_term: Spasticity
    term:
      id: HP:0001257
      label: Spasticity
  evidence:
  - reference: PMID:22627108
    reference_title: Functions of plasmalogen lipids in health and disease.
    supports: NO_EVIDENCE
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Their biosynthesis starts in peroxisomes, and defects at these steps cause
      the malformation syndrome, Rhizomelic Chondrodysplasia Punctata (RCDP).
    explanation: >-
      No disease-modifying therapy exists for RCDP; management is supportive
      care by clinical convention. This snippet describes the disease mechanism,
      not a treatment intervention.
- name: Cataract Extraction
  description: >-
    Surgical removal of congenital cataracts can restore and/or preserve vision.
  treatment_term:
    preferred_term: surgical procedure
    term:
      id: MAXO:0000004
      label: surgical procedure
  target_phenotypes:
  - preferred_term: Cataract
    term:
      id: HP:0000518
      label: Cataract
  evidence:
  - reference: PMID:25439727
    reference_title: A peroxisomal disorder of severe intellectual disability, epilepsy, and cataracts due to fatty acyl-CoA reductase 1 deficiency.
    supports: NO_EVIDENCE
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      severe intellectual disability, early-onset epilepsy, microcephaly,
      congenital cataracts, growth retardation, and spasticity.
    explanation: >-
      Congenital cataracts are present in RCDP and are amenable to surgical
      extraction by standard ophthalmologic practice; this snippet establishes
      cataract as a feature but does not describe the surgical intervention.
differential_diagnoses:
- name: Rhizomelic Chondrodysplasia Punctata Type 1
  disease_term:
    preferred_term: rhizomelic chondrodysplasia punctata type 1
    term:
      id: MONDO:0008972
      label: rhizomelic chondrodysplasia punctata type 1
  description: >-
    RCDP1 is caused by biallelic PEX7 variants that abolish the cytosolic PTS2
    import receptor, producing a generalized loss of PTS2 cargo — including both
    plasmalogen-synthesis enzymes and phytanoyl-CoA hydroxylase — so plasmalogens
    are deficient AND phytanic acid accumulates.
  distinguishing_features:
  - >-
    RCDP1 is a PTS2 import-receptor defect with combined plasmalogen deficiency
    and elevated phytanic acid; RCDP2/3/4 are single-enzyme plasmalogen-synthesis
    defects with normal phytanic acid.
- name: Rhizomelic Chondrodysplasia Punctata Type 5
  disease_term:
    preferred_term: rhizomelic chondrodysplasia punctata type 5
    term:
      id: MONDO:0014743
      label: rhizomelic chondrodysplasia punctata type 5
  description: >-
    RCDP5 results from PEX5 variants affecting the PEX7-binding domain, which
    impairs PTS2 import similarly to RCDP1 (an import-receptor lesion), not a
    single ether-lipid enzyme defect.
  distinguishing_features:
  - >-
    RCDP5 is a PEX5 PTS2-binding-domain import defect; the single-enzyme forms in
    this entry leave peroxisomal import intact.
- name: FAR1 Upregulation Disorder (gain-of-function)
  disease_term:
    preferred_term: fatty acyl-CoA reductase 1 upregulation
    term:
      id: MONDO:0100230
      label: fatty acyl-CoA reductase 1 upregulation
  description: >-
    Autosomal dominant gain-of-function FAR1 variants disrupt the feedback
    control of FAR1 stability, causing plasmalogen ELEVATION rather than
    deficiency. Despite the opposite biochemical direction, affected individuals
    share a neurodevelopmental phenotype with cataracts and spasticity,
    illustrating the tight homeostatic requirement for plasmalogen dosage.
  distinguishing_features:
  - >-
    FAR1 gain-of-function (AD) causes plasmalogen elevation via impaired FAR1
    feedback regulation; RCDP4 is FAR1 loss-of-function (AR) causing plasmalogen
    deficiency.
- name: Zellweger Spectrum Disorders
  disease_term:
    preferred_term: Zellweger spectrum disorders
    term:
      id: MONDO:0019609
      label: Zellweger spectrum disorders
  description: >-
    ZSD results from generalized peroxisomal matrix-import failure (PEX genes)
    affecting both PTS1 and PTS2 cargo, producing VLCFA accumulation alongside
    plasmalogen and phytanic/bile-acid abnormalities.
  distinguishing_features:
  - >-
    ZSD has generalized peroxisomal dysfunction with elevated VLCFA; the
    single-enzyme RCDP forms have an isolated plasmalogen-synthesis defect with
    normal VLCFA and phytanic acid.
notes: >-
  Scope/anchor note for reviewers: there is no MONDO grouping term that cleanly
  covers RCDP types 2-4 while excluding the import-receptor forms (RCDP1/PEX7,
  RCDP5/PEX5), so this entry is anchored to the clinical umbrella
  MONDO:0015776 (broadMatch) and scoped via subtypes to the single-enzyme
  plasmalogen-synthesis defects GNPAT (RCDP2), AGPS (RCDP3), and FAR1 deficiency
  (RCDP4). Two corrections were made versus the auto-generated issue scaffold on
  #4181: (1) MONDO:0014743 "rhizomelic chondrodysplasia punctata type 5" is a
  PEX5 PTS2-binding-domain import defect (HGNC:9719 PEX5), not a FAR1 disorder,
  so it is modeled as a differential rather than a plasmalogen-synthesis subtype;
  (2) MONDO:0100230 "FAR1 upregulation" is an autosomal dominant gain-of-function
  disorder causing plasmalogen ELEVATION (the mechanistic inverse of the
  deficiency convergence) and is therefore a differential, not a co-equal
  deficiency subtype.