Lymphomatoid Granulomatosis

Cancer MONDO:0019466 Pathograph 8 Show in embeddings browser Epstein-Barr virus-associated lymphoproliferative disorder Diffuse large B-cell lymphoma

Lymphomatoid granulomatosis is a rare Epstein-Barr virus-driven B-cell lymphoproliferative disease in which EBV-positive atypical B cells accumulate in extranodal tissue - always the lung, often skin, central nervous system, liver and kidney - inside a dense, angiocentric and angiodestructive reactive T-cell infiltrate. Nodal and bone marrow involvement is so rare that finding it argues for a different diagnosis. The disease is understood as a failure of EBV immunosurveillance rather than as a conventional oncogenic-driver malignancy: no recurrent chromosomal lesion has been identified, patients have measurably depressed circulating T cells with the deficit falling mainly on the CD8-positive compartment, and the vascular damage and necrosis that give the disease its name are attributed to the host response to EBV rather than to the neoplastic cells. Histologic grade, defined by the number and density of EBV-positive atypical B cells, tracks clonality and is the axis on which treatment turns: low-grade disease is treated as immune-dependent with interferon alfa-2b, high-grade disease as immune-independent with chemoimmunotherapy.

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1
Mappings
5
Pathophys.
2
Histopath.
4
Phenotypes
3
Gaps
8
Pathograph
4
Medical Actions
3
Subtypes
2
Differentials
1
Trials
1
Deep Research
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Classifications

ICD-O Morphology
Lymphoma
Harrison's Part
ONCOLOGY HEMATOLOGY RESPIRATORY
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Mappings

MONDO
MONDO:0019466 lymphomatoid granulomatosis
skos:exactMatch MONDO
Primary MONDO disease identifier for this entry.

Subtypes

3
Grade 1 (low grade) MONDO:0859747
Polymorphous infiltrate with rare atypical medium-to-large lymphoid cells and a paucity of EBV-positive cells, fewer than five per high-power field. Necrosis is focal or absent and the infiltrate is usually polyclonal - only about 8% of grade 1 lesions carry a clonal immunoglobulin rearrangement. This is the grade treated as immune-dependent, and the grade hardest to diagnose because the EBV-positive cells can be sparse or absent.
Show evidence (1 reference)
PMID:25321327 SUPPORT Human Clinical
"Immunoglobulin gene rearrangement studies were performed, and a higher percentage of clonal rearrangements were seen in LYG grade 2 (50%) and grade 3 (69%) as compared with grade 1 (8%)."
Gives the grade 1 clonality figure that distinguishes it from the higher grades.
Grade 2 (low grade) MONDO:0859748
More numerous large atypical cells, sometimes reminiscent of Hodgkin/Reed-Sternberg cells, with near-universal EBER positivity at five to twenty cells per high-power field and more necrosis than grade 1. Half of grade 2 lesions are clonal. Grouped with grade 1 as low-grade, immune-dependent disease for treatment purposes.
Show evidence (1 reference)
PMID:32107539 SUPPORT Other
"In accordance with the World Health Organization classification, grade 1 lesions were associated with rare atypical medium-to-large lymphoid cells, whereas a greater number of large atypical cells, occasionally reminiscent of Hodgkin/Reed-Sternberg cells, were frequently seen in grade 2 and 3 lesions."
Describes the cytologic difference between grade 1 and the higher grades.
Grade 3 (high grade) MONDO:0859749
Sheets of large atypical EBV-positive B cells, more than fifty and often more than a hundred per high-power field, with extensive coagulative necrosis. About 69% are clonal. This grade behaves as, and is treated as, an aggressive EBV-positive large B-cell lymphoma - immune-independent disease.
Show evidence (1 reference)
PMID:32107539 SUPPORT Other
"In contrast, near universal expression of EBER was seen in grade 2 (5-20 and occasionally up to 50 cells per high-power field) and grade 3 (>50 and often >100 cells per high power field) lesions"
Gives the EBER density thresholds that define grade 2 and grade 3.
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Discussions and Knowledge Gaps

3
What is the specific immune defect that permits lymphomatoid granulomatosis in patients with no recognised primary immunodeficiency?
KNOWLEDGE GAP OPEN lyg_immune_defect_unidentified
The upstream node of this entry is a defect that has been measured only as an aggregate: reduced circulating T cells, falling more on the CD8 compartment, in patients who have no diagnosed immunodeficiency syndrome. It is inferred to be pre-existing and is described by its own authors as hypothesised. No causal gene, no functional assay and no biomarker identifies it in an individual patient, so the entry has a trigger node it cannot yet characterise molecularly.
Show evidence (1 reference)
PMID:32107539 SUPPORT Human Clinical
"Taken together, these results suggest that the immunologic deficits are likely preexistent and that a quantitative and/or qualitative defect in mainly CD8+cytotoxic T cells may be a prerequisite for disease."
The hedged language is the point: this is the strongest available characterisation of the defect.
How should treatment be allocated when the grading that selects the treatment arm is not reproducible between observers?
KNOWLEDGE GAP OPEN lyg_grading_reproducibility
Grade decides whether a patient receives interferon alfa-2b or chemoimmunotherapy, and the toxicity difference between those arms is large - serious adverse events in 25% against 64%. The pathologists who defined the grading system record in the same paper that it is inconsistent and poorly reproducible between observers, with grade 1 the hardest because the EBV-positive cells can be sparse or absent. No reproducibility study, quantitative EBER threshold or adjunct molecular assay has been published to close the gap.
Show evidence (2 references)
PMID:25321327 SUPPORT Human Clinical
"However, in practice, the grading can be inconsistent and poorly reproducible among observers."
States the reproducibility problem directly.
PMID:25321327 SUPPORT Human Clinical
"The greatest difficulty for the diagnosis of LYG has been with grade 1 lesions because of the possible absence or paucity of EBV-positive atypical B cells."
Identifies grade 1 as the specific point of failure, which is also the grade that selects the low-toxicity arm.
Do humanized-mouse models of EBV lymphoproliferation actually model lymphomatoid granulomatosis, or only the generic EBV-plus-immunosuppression axis?
HUMAN MODEL MISMATCH OPEN lyg_no_model_system
No animal or in vitro model of this disease exists. Humanized mice reconstituted with human haematopoietic cells develop EBV-associated B-cell lymphoproliferation, and immunosuppression increases its frequency, so they model the upstream axis. They do not reproduce what actually defines the disease - the angiocentric and angiodestructive architecture, the lung-skin-CNS distribution, or the CD4-predominant reactive microenvironment. The entry therefore records no animal_models, and the tissue-level node has no experimental system behind it. This is flagged as a model mismatch rather than a plain knowledge gap because evidence exists in a model system whose fidelity to the human tissue phenotype is the open question.
Show evidence (2 references)
PMID:25436886 SUPPORT Model Organism
"Thus, many aspects of human EBV infection, including associated diseases (e.g., lymphoproliferative disease, hemophagocytic lymphohistiocytosis and erosive arthritis resembling RA), latent infection, and T-cell-mediated and humoral immune responses have been successfully reproduced in humanized mice."
Establishes what the available models do reproduce - generic EBV-driven lymphoproliferation and immune control - which is the upstream axis of this disease and not its defining tissue phenotype.
PMID:32251475 SUPPORT Model Organism
"We found that FK506 treatment of EBV-infected mice led to an elevated viral burden, more frequent tumor formation and diminished EBV-induced T cell responses, indicative of reduced EBV-specific immune control."
Shows that the immunosuppression-plus-EBV axis is modellable, and that the modelled endpoint is post-transplant-style lymphoproliferation rather than an angiocentric, lung-predominant lesion.

Pathophysiology

5
Defective EBV Immunosurveillance
Latent EBV, carried by most of the adult population, escapes control in a host whose cell-mediated surveillance of EBV-infected B cells is quantitatively or qualitatively impaired. Every patient treated at the reporting national centre had moderately to severely reduced circulating CD3-positive T cells with the deficit falling more heavily on the CD8-positive compartment, and none had a recognised primary immunodeficiency - so the defect is inferred to be pre-existing and subtle rather than syndromic. Recognised syndromic and iatrogenic causes of the same defect exist and predispose to the disease.
CD8-positive, alpha-beta T cell CL:0000625 Cell Ontology (CL) Relation: this pathophysiological event involves this cell type This pathophysiological event involves CD8-positive, alpha-beta T cell (CL:0000625). CL:0000625 is a cell type from the Cell Ontology.
viral latency GO:0019042 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves increased viral latency (GO:0019042). GO:0019042 is a biological process from the Gene Ontology. ↑ INCREASED T cell mediated immunity GO:0002456 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves decreased T cell mediated immunity (GO:0002456). GO:0002456 is a biological process from the Gene Ontology. ↓ DECREASED
Show evidence (2 references)
PMID:32107539 SUPPORT Human Clinical
"Taken together, these results suggest that the immunologic deficits are likely preexistent and that a quantitative and/or qualitative defect in mainly CD8+cytotoxic T cells may be a prerequisite for disease."
States the CD8-centred surveillance defect and its position upstream of disease.
PMID:25321327 SUPPORT Human Clinical
"More recently, it has been hypothesized that patients with LYG have defective immune surveillance of EBV-infected B cells particularly by the CD8-positive T cells"
Independent statement of the same model from the pathology series.
Outgrowth of EBV-Positive Atypical B Cells
EBV-infected B cells expressing latent viral proteins proliferate and accumulate in extranodal tissue. Their number and density per high-power field is precisely what histologic grade measures, so this node is the one the grading system quantifies. Nodal and bone marrow compartments are spared, which is one of the features that separates the disease from other EBV-positive B-cell lymphoproliferations.
EBV-positive atypical B cell CL:0000236 Cell Ontology (CL) Relation: this pathophysiological event involves this cell type This pathophysiological event involves EBV-positive atypical B cell, annotated with B cell (CL:0000236). CL:0000236 is a cell type from the Cell Ontology.
cell population proliferation GO:0008283 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves increased cell population proliferation (GO:0008283). GO:0008283 is a biological process from the Gene Ontology. ↑ INCREASED
lung UBERON:0002048 Uberon multi-species anatomy ontology (UBERON) Relation: this pathophysiological event occurs in this anatomical location This pathophysiological event occurs in lung (UBERON:0002048). UBERON:0002048 is an anatomical location from the Uberon multi-species anatomy ontology.
Show evidence (2 references)
PMID:32107539 SUPPORT Other
"Pathologically, LYG is graded by the number and density of EBV+ atypical B cells, and other characteristic findings include an angioinvasive/angiodestructive reactive T-cell infiltrate and various degrees of necrosis."
Identifies the EBV-positive atypical B-cell population as the graded quantity.
PMID:32107539 SUPPORT Other
"Clinically, LYG universally involves the lungs with other common extranodal sites, including skin, central nervous system, liver, and kidneys. Nodal and/or bone marrow involvement is extremely rare and, if present, suggests an alternative diagnosis."
Establishes the extranodal distribution and the diagnostic weight of nodal sparing.
Ineffective Angiocentric Reactive T-cell Response
A dense reactive T-cell infiltrate, CD4-predominant rather than CD8, gathers around and invades vessel walls without clearing the EBV-positive B cells. The interferon-inducible chemokines CXCL9 and CXCL10 localise to reactive cells in viable tissue at the edge of necrotic areas rather than to the malignant cells, which is the basis for attributing the vascular damage to the host response rather than to the tumour. The vascular injury is angiocentric and angiodestructive but is not a primary vasculitis - the vessel wall is damaged by lymphocytic invasion, not by an immune-mediated attack on the wall itself.
CD4-positive, alpha-beta T cell CL:0000624 Cell Ontology (CL) Relation: this pathophysiological event involves this cell type This pathophysiological event involves CD4-positive, alpha-beta T cell (CL:0000624). CL:0000624 is a cell type from the Cell Ontology.
chemokine-mediated signaling pathway GO:0070098 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves increased chemokine-mediated signaling pathway (GO:0070098). GO:0070098 is a biological process from the Gene Ontology. ↑ INCREASED inflammatory response GO:0006954 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves increased inflammatory response (GO:0006954). GO:0006954 is a biological process from the Gene Ontology. ↑ INCREASED
blood vessel UBERON:0001981 Uberon multi-species anatomy ontology (UBERON) Relation: this pathophysiological event occurs in this anatomical location This pathophysiological event occurs in blood vessel (UBERON:0001981). UBERON:0001981 is an anatomical location from the Uberon multi-species anatomy ontology.
Show evidence (3 references)
PMID:32107539 SUPPORT Other
"CXCL9 and CXCL10 localize to the reactive cells in the viable tissue surrounding areas of necrosis, suggesting that cells in the tumor microenvironment and not the malignant cells themselves are the principal source of chemokines, resulting in vascular damage and necrosis."
Locates the chemokine source in the reactive microenvironment, which is what makes this node distinct from the B-cell node upstream of it.
PMID:32107539 SUPPORT Other
"Thus, the host immune response to EBV is a principal cause of the vasculitic changes that are such a typical feature of the disease."
States directly that the host response, not the neoplastic clone, drives the vascular pathology.
PMID:32107539 SUPPORT Other
"Most of the angiocentric lymphocytes are CD3+T cells, with CD4+cells representing the predominant subtype in most cases."
Establishes the CD4-predominant composition of the angiocentric infiltrate.
Coagulative Necrosis and Multi-organ Tissue Destruction
Coagulative necrosis follows the vascular damage and is present at every grade, focal in grade 1 and often extensive in grades 2 and 3. In the lung this produces the bilateral nodules, sometimes cavitating, that define the radiologic picture; in skin, central nervous system, liver and kidney it produces the corresponding extranodal lesions.
cell death GO:0008219 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves increased cell death (GO:0008219). GO:0008219 is a biological process from the Gene Ontology. ↑ INCREASED
lung UBERON:0002048 Uberon multi-species anatomy ontology (UBERON) Relation: this pathophysiological event occurs in this anatomical location This pathophysiological event occurs in lung (UBERON:0002048). UBERON:0002048 is an anatomical location from the Uberon multi-species anatomy ontology. brain UBERON:0000955 Uberon multi-species anatomy ontology (UBERON) Relation: this pathophysiological event occurs in this anatomical location This pathophysiological event occurs in brain (UBERON:0000955). UBERON:0000955 is an anatomical location from the Uberon multi-species anatomy ontology. skin of body UBERON:0002097 Uberon multi-species anatomy ontology (UBERON) Relation: this pathophysiological event occurs in this anatomical location This pathophysiological event occurs in skin of body (UBERON:0002097). UBERON:0002097 is an anatomical location from the Uberon multi-species anatomy ontology. kidney UBERON:0002113 Uberon multi-species anatomy ontology (UBERON) Relation: this pathophysiological event occurs in this anatomical location This pathophysiological event occurs in kidney (UBERON:0002113). UBERON:0002113 is an anatomical location from the Uberon multi-species anatomy ontology. liver UBERON:0002107 Uberon multi-species anatomy ontology (UBERON) Relation: this pathophysiological event occurs in this anatomical location This pathophysiological event occurs in liver (UBERON:0002107). UBERON:0002107 is an anatomical location from the Uberon multi-species anatomy ontology.
Show evidence (2 references)
PMID:25321327 SUPPORT Human Clinical
"Necrosis was seen in all grades, with a greater degree in high-grade lesions."
Establishes necrosis as a feature of every grade with a grade-dependent extent.
PMID:25321327 SUPPORT Human Clinical
"We reviewed 122 biopsies; the most common site was lung (73%), followed by skin/subcutaneous tissue (17%); other sites included kidney, nasal cavity, gastrointestinal tract, conjunctiva, liver, and adrenal gland."
Documents the organ distribution of biopsy-proven tissue destruction.
Clonal Selection and High-Grade Transformation
With continued proliferation under a persistent surveillance defect, the EBV-infected B-cell population shifts from polyclonal to monoclonal. Immunoglobulin gene rearrangement is clonal in 8% of grade 1, 50% of grade 2 and 69% of grade 3 lesions - a monotonic gradient that is the molecular reading of the grading system. At grade 3 the lesion is no longer separable from an EBV-positive large B-cell lymphoma, which is why the treatment strategy switches from restoring immunity to killing the clone. No recurrent chromosomal abnormality has been found, so this transformation is not anchored to a known cytogenetic driver.
EBV-positive atypical B cell CL:0000236 Cell Ontology (CL) Relation: this pathophysiological event involves this cell type This pathophysiological event involves EBV-positive atypical B cell, annotated with B cell (CL:0000236). CL:0000236 is a cell type from the Cell Ontology.
cell population proliferation GO:0008283 Gene Ontology (GO) Relation: this pathophysiological event involves this biological process This pathophysiological event involves increased cell population proliferation (GO:0008283). GO:0008283 is a biological process from the Gene Ontology. ↑ INCREASED
Show evidence (2 references)
PMID:25321327 SUPPORT Human Clinical
"Immunoglobulin gene rearrangement studies were performed, and a higher percentage of clonal rearrangements were seen in LYG grade 2 (50%) and grade 3 (69%) as compared with grade 1 (8%)."
Provides the grade-clonality gradient that this node describes.
PMID:32107539 SUPPORT Other
"This shift in clonality from polyclonal to monoclonal disease seen with increased histologic grade most likely represents the selective transformation of EBV-infected B-cell clones"
States the clonal-selection interpretation of that gradient, hedged by its authors as the likely explanation, which is why this node is provisional.

Histopathology

2
Angiocentric and angiodestructive polymorphous infiltrate
A polymorphous lymphoid infiltrate centred on and invading vessels, rich in reactive T cells, containing large atypical EBV-positive B cells, with coagulative necrosis. This combination is the diagnostic finding and is what separates the disease from other EBV-positive B-cell lymphoproliferations.
Show evidence (1 reference)
PMID:25321327 SUPPORT Human Clinical
"Histologically, the lesions showed angiocentricity, were rich in T cells, had large atypical B cells, and were positive for EBV."
States the four features that together define the diagnostic histology.
Grade assignment by EBER-positive B-cell density
Grade is assigned from the number and density of EBER-positive atypical B cells and the extent of coagulative necrosis, predominantly on the diagnostic lung biopsy. In the reference series grades were distributed 30% grade 1, 22% grade 2 and 48% grade 3. Grading is acknowledged to be inconsistent and poorly reproducible between observers, which matters because it selects the treatment arm.
Show evidence (2 references)
PMID:25321327 SUPPORT Human Clinical
"Grading was performed predominantly on the lung biopsy at diagnosis; they were distributed as follows: LYG grade 1 (30%), grade 2 (22%), and grade 3 (48%)."
Gives the grade distribution and confirms the lung biopsy as the grading substrate.
PMID:25321327 SUPPORT Human Clinical
"However, in practice, the grading can be inconsistent and poorly reproducible among observers."
Records the reproducibility limitation of the grading system that drives therapy.

Pathograph

Use the checkboxes to hide or show graph categories. Hover nodes for evidence and cross-linked metadata.
Pathograph: causal mechanism network for Lymphomatoid Granulomatosis Interactive directed graph showing how pathophysiology mechanisms, phenotypes, genetic factors and variants, experimental models, environmental triggers, and treatments relate through causal and linked edges.

Phenotypes

4
Integument 1
Cutaneous Nodules and Plaques Skin nodule HP:0200036 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Skin nodule (HP:0200036). HP:0200036 is a phenotype from the Human Phenotype Ontology.
Show evidence (2 references)
PMID:25321327 SUPPORT Human Clinical
"We reviewed 122 biopsies; the most common site was lung (73%), followed by skin/subcutaneous tissue (17%); other sites included kidney, nasal cavity, gastrointestinal tract, conjunctiva, liver, and adrenal gland."
Gives skin and subcutaneous tissue as the second most frequently biopsied site.
PMID:32107539 SUPPORT Other
"For this reason, skin lesions alone are inadequate to establish a definitive diagnosis of LYG."
Supports marking the cutaneous phenotype as non-diagnostic on its own.
Respiratory 1
Dyspnea HP:0002094 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Dyspnea (HP:0002094). HP:0002094 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:23006954 SUPPORT INDIRECT Other
"Lymphomatoid granulomatosis can lead to progressive pulmonary failure, central nervous system disease, or progression to overt EBV-positive lymphoma without appropriate recognition and management."
Supports progressive pulmonary compromise as a disease consequence. The review does not enumerate individual respiratory symptoms, so breathlessness follows by inference and the item is marked INDIRECT.
Other 2
Bilateral Pulmonary Nodules VERY_FREQUENT HP:0033608 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Pulmonary nodule (HP:0033608). HP:0033608 is a phenotype from the Human Phenotype Ontology.
Show evidence (2 references)
PMID:25321327 SUPPORT Human Clinical
"Clinically, all patients had lung involvement (100%), with the next most common site being the central nervous system (38%). No patient had nodal or bone marrow disease."
Gives the 100% lung involvement figure in the largest pathology series.
PMID:23006954 SUPPORT Other
"It is usually a progressive disorder that virtually always involves the lung and characteristically presents as bilateral pulmonary nodules."
Confirms bilateral nodules as the characteristic pulmonary presentation.
Central Nervous System Involvement FREQUENT Morphological central nervous system abnormality HP:0002011 Human Phenotype Ontology (HP) Relation: this clinical feature is this phenotype This clinical feature is Morphological central nervous system abnormality (HP:0002011). HP:0002011 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
PMID:25321327 SUPPORT Human Clinical
"Clinically, all patients had lung involvement (100%), with the next most common site being the central nervous system (38%). No patient had nodal or bone marrow disease."
Gives the 38% frequency of central nervous system involvement supporting the FREQUENT band.
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Medical Actions

4
Interferon Alfa-2b
Action: PharmacotherapyNCI Thesaurus (NCIT) Relation: this treatment is this clinical intervention This treatment is Pharmacotherapy (NCIT:C15986). NCIT:C15986 is a clinical intervention from the NCI Thesaurus. NCIT:C15986
Agent: recombinant interferon alfa-2b NCIT:C1953 NCI Thesaurus (NCIT) Relation: this treatment uses this therapeutic agent This treatment uses recombinant interferon alfa-2b (NCIT:C1953). NCIT:C1953 is a therapeutic agent from the NCI Thesaurus.
Dose-escalated subcutaneous interferon alfa-2b, from 7.5 million international units three times weekly and continued for up to a year past best response, for low-grade disease. The rationale is explicitly mechanistic: low-grade disease is treated as immune-dependent, so the intervention augments the host response to EBV rather than killing the clone. In the phase 2 trial it produced complete responses in 61% of evaluable patients and was substantially better tolerated than chemotherapy.
Mechanism Target:
Defective EBV Immunosurveillance — Augments the host immune response to EBV, which is the defect this node records.
Show evidence (3 references)
PMID:37011643 SUPPORT Human Clinical
"After initial treatment with interferon alfa-2b, the overall response was 64% (28 of 44 evaluable patients) with 61% (27 of 44) having a complete response"
Gives the response rates from the pivotal prospective trial.
PMID:37011643 SUPPORT Human Clinical
"Interferon alfa-2b is efficacious for treating low-grade lymphomatoid granulomatosis and hence reducing progression to high-grade disease, whereas patients with high-grade lymphomatoid granulomatosis showed expected responses to chemotherapy."
The trial's own conclusion, including the claim that treating low-grade disease reduces progression to high grade.
PMID:32107539 SUPPORT Other
"Methods of augmenting the immune response to EBV in low-grade LYG include treatment with interferon-α2b, whereas high-grade disease requires immunochemotherapy."
States the mechanistic rationale that makes this an immune-directed rather than cytotoxic treatment.
DA-EPOCH-R
Action: chemotherapyNCI Thesaurus (NCIT) Relation: this treatment is this clinical intervention This treatment is chemotherapy (NCIT:C15632). NCIT:C15632 is a clinical intervention from the NCI Thesaurus. Ontology label: Chemotherapy NCIT:C15632 Regimen: dose-adjusted EPOCH-R regimenNCI Thesaurus (NCIT) Relation: this regimen component is this clinical intervention This regimen component is dose-adjusted EPOCH-R regimen (NCIT:C140097). NCIT:C140097 is a clinical intervention from the NCI Thesaurus. Ontology label: Dose-adjusted EPOCH-R Regimen NCIT:C140097
Agent: etoposide CHEBI:4911 Chemical Entities of Biological Interest (CHEBI) Relation: this treatment uses this therapeutic agent This treatment uses etoposide (CHEBI:4911). CHEBI:4911 is a therapeutic agent from Chemical Entities of Biological Interest. prednisone CHEBI:8382 Chemical Entities of Biological Interest (CHEBI) Relation: this treatment uses this therapeutic agent This treatment uses prednisone (CHEBI:8382). CHEBI:8382 is a therapeutic agent from Chemical Entities of Biological Interest. vincristine CHEBI:28445 Chemical Entities of Biological Interest (CHEBI) Relation: this treatment uses this therapeutic agent This treatment uses vincristine (CHEBI:28445). CHEBI:28445 is a therapeutic agent from Chemical Entities of Biological Interest. cyclophosphamide CHEBI:4027 Chemical Entities of Biological Interest (CHEBI) Relation: this treatment uses this therapeutic agent This treatment uses cyclophosphamide (CHEBI:4027). CHEBI:4027 is a therapeutic agent from Chemical Entities of Biological Interest. doxorubicin CHEBI:28748 Chemical Entities of Biological Interest (CHEBI) Relation: this treatment uses this therapeutic agent This treatment uses doxorubicin (CHEBI:28748). CHEBI:28748 is a therapeutic agent from Chemical Entities of Biological Interest. rituximab NCIT:C1702 NCI Thesaurus (NCIT) Relation: this treatment uses this therapeutic agent This treatment uses rituximab (NCIT:C1702). NCIT:C1702 is a therapeutic agent from the NCI Thesaurus.
Six three-weekly cycles of dose-adjusted etoposide, prednisone, vincristine, cyclophosphamide, doxorubicin and rituximab for high-grade disease, which is treated as immune-independent. Complete responses were seen in 47% of evaluable patients. Toxicity is the trade-off: serious adverse events occurred in 64% of chemotherapy-treated patients against 25% of those on interferon alfa-2b.
Mechanism Target:
Clonal Selection and High-Grade Transformation — Cytotoxic and anti-CD20 therapy directed at the transformed clone that defines high-grade disease.
Show evidence (2 references)
PMID:37011643 SUPPORT Human Clinical
"After initial treatment with DA-EPOCH-R, the overall response was 76% (13 of 17 evaluable patients) with 47% (eight of 17) having a complete response"
Gives the response rates for the high-grade arm of the trial.
PMID:37011643 SUPPORT Human Clinical
"Serious adverse events occurred in 13 (25%) of 51 patients receiving treatment with interferon alfa-2b and 21 (64%) of 33 patients receiving DA-EPOCH-R, with five treatment-related deaths: one thromboembolic, one infection, and one haemophagocytic syndrome with interferon alfa-2b, and one..."
Quantifies the toxicity difference between the two arms, including treatment-related deaths in both.
Crossover Between Treatment Arms
Action: Therapeutic ProcedureNCI Thesaurus (NCIT) Relation: this treatment is this clinical intervention This treatment is Therapeutic Procedure (NCIT:C49236). NCIT:C49236 is a clinical intervention from the NCI Thesaurus. NCIT:C49236
Patients with residual or progressive disease after initial therapy cross over to the other arm. This is not a salvage improvisation but a designed consequence of the model: the underlying EBV surveillance defect persists through chemotherapy, so high-grade disease can recur as low-grade disease and be treated with interferon, and low-grade disease can transform under immune modulation and need chemotherapy.
Show evidence (2 references)
PMID:37011643 SUPPORT Human Clinical
"Patients with residual or progressive disease after initial therapy crossed over to alternative therapy."
Documents crossover as part of the prospective trial design.
PMID:32107539 SUPPORT Other
"Given the underlying defective immune surveillance of EBV, patients with high-grade disease may have a recurrence in the form of low-grade disease after immunochemotherapy, and those with low-grade disease may progress to high-grade disease after immune modulation, which can be effectively..."
Gives the mechanistic reason crossover is expected rather than exceptional.
Hematopoietic Stem Cell Transplantation
Action: Hematopoietic Cell TransplantationNCI Thesaurus (NCIT) Relation: this treatment is this clinical intervention This treatment is Hematopoietic Cell Transplantation (NCIT:C15431). NCIT:C15431 is a clinical intervention from the NCI Thesaurus. NCIT:C15431
Considered for primary refractory disease or multiple relapses. Its efficacy in this disease is explicitly described as not well established, so it is recorded here as an option rather than a standard.
Show evidence (1 reference)
PMID:32107539 SUPPORT DIRECT Other
"In patients with primary refractory disease or in those with multiple relapses, hematopoietic stem cell transplantation may be considered, but its efficacy is not well established."
Supports transplantation as a considered option while explicitly declining to claim established efficacy.
🔬

Diagnosis

2
Tissue biopsy with EBER in situ hybridization
Definitive diagnosis needs tissue, usually a lung biopsy, showing the angiocentric polymorphous infiltrate with EBER-positive atypical B cells. Immunohistochemistry for CD20, CD3, CD4, CD8 and LMP1 and immunoglobulin gene rearrangement studies support the diagnosis and set the grade.
Show evidence (2 references)
PMID:25321327 SUPPORT Human Clinical
"LYG is a distinct entity that can usually be differentiated from other EBV-associated B-cell lymphoproliferative disorders on the basis of the combination of clinical presentation, histology, and EBV studies."
States that the diagnosis rests on the combination of clinical picture, histology and EBV studies.
PMID:25321327 SUPPORT Human Clinical
"Grading of these lesions is important because it dictates the treatment choice."
Explains why grading, and therefore adequate tissue, is a diagnostic requirement rather than an academic exercise.
Recognition of diagnostic delay
The combination of rarity and a nonspecific presentation - cough, breathlessness, fever, pulmonary nodules - routinely delays diagnosis, and the differential includes both infection and vasculitis.
Show evidence (1 reference)
PMID:23006954 SUPPORT Other
"The rareness of LYG in conjunction with its nonspecific presentation contributes to delays in diagnosis in many situations."
Documents diagnostic delay as a recognised feature of the disease.
📈

Progression

3
Untreated or steroid-treated natural history
Historically poor. Before grade-stratified therapy, patients treated with steroids and/or chemotherapy had a median survival of about 14 months, and median overall survival across the disease was under two years.
Show evidence (2 references)
PMID:25321327 SUPPORT Human Clinical
"In regards to treatment, historical outcomes of patients treated with steroids and/or chemotherapy have been poor (median survivals of 14 months)"
Gives the historical median survival figure for the pre-stratification era.
PMID:37011643 SUPPORT Human Clinical
"Lymphomatoid granulomatosis is a rare Epstein-Barr virus-associated B-cell lymphoproliferative disorder with a median overall survival of less than 2 years."
The trial's own statement of the survival baseline it set out to improve.
Grade transformation in both directions
Grade is not a fixed property of a patient. Low-grade disease can progress to high-grade disease after immune modulation, and high-grade disease can recur as low-grade disease after immunochemotherapy. Both directions are managed by crossing over to the other arm of therapy, which is a direct clinical consequence of the shared underlying defect in EBV surveillance.
Show evidence (1 reference)
PMID:32107539 SUPPORT Other
"Given the underlying defective immune surveillance of EBV, patients with high-grade disease may have a recurrence in the form of low-grade disease after immunochemotherapy, and those with low-grade disease may progress to high-grade disease after immune modulation, which can be effectively..."
States the bidirectional grade shift and its mechanistic explanation.
Progression to overt EBV-positive lymphoma or organ failure
Without recognition and grade-appropriate treatment the disease can progress to pulmonary failure, central nervous system disease, or frank EBV-positive lymphoma.
Show evidence (1 reference)
PMID:23006954 SUPPORT Other
"Lymphomatoid granulomatosis can lead to progressive pulmonary failure, central nervous system disease, or progression to overt EBV-positive lymphoma without appropriate recognition and management."
Names the three endpoints of untreated progression.
📊

Prevalence

1
Worldwide
Unknown Ultra Rare
No incidence or prevalence estimate has been published. Cohort size is the only available proxy: the largest single-institution pathology series covers 55 patients referred to one national centre over 15 years, and the pivotal prospective trial accrued 67 patients over 28 years.
Show evidence (1 reference)
PMID:25321327 SUPPORT Human Clinical
"We reviewed the biopsies of 55 patients with LYG who were referred for a prospective trial at the National Cancer Institute (1995 to 2010) and evaluated the histologic, immunohistochemical, in situ hybridization, and molecular findings of these biopsies in conjunction with clinical information."
Establishes the scale of the largest available series, which is the only quantitative handle on how rare the disease is.
🦠

Infectious Agent

1
Epstein-Barr virus
EBV is present in the atypical B cells in essentially every case and is treated as the transforming agent rather than a bystander. Patients have serologic evidence of past exposure but not of acute infection, and the measured viral load is low - the abnormality is control of a common latent infection, not an unusual exposure.
Epstein-Barr virus NCBITaxon:10376 NCBI Taxonomy (NCBITaxon)
Show evidence (2 references)
PMID:25321327 SUPPORT Human Clinical
"All patients had past EBV exposure by serology but with a low median EBV viral load."
Shows that the relevant abnormality is EBV control rather than an acute or high-burden infection.
PMID:32107539 SUPPORT Other
"Lymphomatoid granulomatosis (LYG) is a rare Epstein-Barr virus (EBV)-driven B-cell lymphoproliferative disease (LPD). This disease is hypothesized to result from defective immune surveillance of EBV, with most patients showing evidence of immune dysfunction, despite no known primary immunodeficiency."
States both the causal role of EBV and the immune-surveillance model this entry is built around.
🔀

Differential Diagnoses

2

Conditions with similar clinical presentations that must be differentiated from Lymphomatoid Granulomatosis:

Other EBV-positive B-cell lymphoproliferative disorders
Overlapping Features EBV-positive diffuse large B-cell lymphoma and the post-transplant lymphoproliferative disorders share EBV positivity and large atypical B cells. The separating features are the angiocentric architecture, the obligatory pulmonary involvement, and the absence of nodal and bone marrow disease.
Distinguishing Features
  • Angiocentric and angiodestructive architecture with a reactive T-cell-rich background
  • Lung involvement in essentially every patient
  • Nodal or bone marrow involvement argues for an alternative diagnosis
Show evidence (1 reference)
PMID:32107539 SUPPORT Other
"Clinically, LYG universally involves the lungs with other common extranodal sites, including skin, central nervous system, liver, and kidneys. Nodal and/or bone marrow involvement is extremely rare and, if present, suggests an alternative diagnosis."
Gives the distribution rule used to separate this disease from other EBV-positive B-cell lymphoproliferations.
T-cell and NK-cell lymphomas of the angiocentric group
Overlapping Features Extranodal NK/T-cell lymphoma of nasal type and peripheral T-cell lymphoma were historically pooled with this disease under the label angiocentric immunoproliferative lesion, and misclassification confounded the older literature. On expert re-review of cases referred as possible lymphomatoid granulomatosis, a fifth turned out to be something else.
Distinguishing Features
  • The neoplastic population is B-cell, not T-cell or NK-cell
  • Expert re-review reclassified 14 of 69 referred cases, including NK/T-cell lymphoma, peripheral T-cell lymphoma and classical Hodgkin lymphoma
Show evidence (2 references)
PMID:25321327 SUPPORT Human Clinical
"The diagnoses on review were: NK/T cell lymphoma, nasal type (1 case), peripheral T-cell lymphoma, not otherwise specified (1 case), classical Hodgkin's lymphoma (4 cases), and EBV positive polymorphic or monomorphic B-cell lymphoma, lacking features of LYG (8 cases)."
Enumerates what referred cases actually turned out to be, which is the practical content of this differential.
PMID:25321327 SUPPORT Human Clinical
"Earlier studies were likely confounded by the inclusion of T and NK cell lymphomas (formerly included in the diagnosis of angiocentric immunoproliferative lesion) with cases of LYG"
Records the historical conflation that makes this differential important when reading older literature.
🔬

Clinical Trials

1
NCT00001379 PHASE_II COMPLETED
Treatment and Natural History Study of Lymphomatoid Granulomatosis. The single-centre trial that established grade-stratified therapy: interferon alfa-2b for grade 1 and 2 disease, DA-EPOCH-R for grade 3, with crossover for residual or progressive disease. Its results are the evidence base for the treatments recorded in this entry.
Target Phenotypes: Pulmonary nodule HP:0033608 Human Phenotype Ontology (HP) Relation: this clinical trial targets this phenotype This clinical trial targets Pulmonary nodule (HP:0033608). HP:0033608 is a phenotype from the Human Phenotype Ontology.
Show evidence (1 reference)
clinicaltrials:NCT00001379 SUPPORT Human Clinical
"If patients have grade 3 disease, they will usually receive etoposide, prednisone, vincristine, cyclophosphamide, and doxorubicin (EPOCH)-rituximab (EPOCH-R) chemotherapy (each letter representing a drug). If patients have grade 1 or 2 disease, they will usually receive alpha interferon."
The trial record states the grade-stratified allocation this entry's treatment section is built on.
{ }

Source YAML

click to show
name: Lymphomatoid Granulomatosis
creation_date: "2026-08-29T16:00:00Z"
category: Cancer
categories:
- Hematologic Malignancy
- B-cell Neoplasm
- EBV-Associated Lymphoproliferative Disorder
synonyms:
- LYG
- angiocentric immunoproliferative lesion
- Liebow granulomatosis
description: >-
  Lymphomatoid granulomatosis is a rare Epstein-Barr virus-driven B-cell
  lymphoproliferative disease in which EBV-positive atypical B cells accumulate
  in extranodal tissue - always the lung, often skin, central nervous system,
  liver and kidney - inside a dense, angiocentric and angiodestructive reactive
  T-cell infiltrate. Nodal and bone marrow involvement is so rare that finding
  it argues for a different diagnosis. The disease is understood as a failure of
  EBV immunosurveillance rather than as a conventional oncogenic-driver
  malignancy: no recurrent chromosomal lesion has been identified, patients have
  measurably depressed circulating T cells with the deficit falling mainly on
  the CD8-positive compartment, and the vascular damage and necrosis that give
  the disease its name are attributed to the host response to EBV rather than to
  the neoplastic cells. Histologic grade, defined by the number and density of
  EBV-positive atypical B cells, tracks clonality and is the axis on which
  treatment turns: low-grade disease is treated as immune-dependent with
  interferon alfa-2b, high-grade disease as immune-independent with
  chemoimmunotherapy.
disease_term:
  preferred_term: lymphomatoid granulomatosis
  term:
    id: MONDO:0019466
    label: lymphomatoid granulomatosis
mappings:
  mondo_mappings:
  - term:
      id: MONDO:0019466
      label: lymphomatoid granulomatosis
    mapping_predicate: skos:exactMatch
    mapping_source: MONDO
    mapping_justification: Primary MONDO disease identifier for this entry.
parents:
- Epstein-Barr virus-associated lymphoproliferative disorder
- Diffuse large B-cell lymphoma
classifications:
  harrisons_chapter:
  - classification_value: ONCOLOGY_HEMATOLOGY
    notes: >-
      A WHO-recognised mature B-cell neoplasm whose management is grade-stratified
      lymphoma therapy.
  - classification_value: RESPIRATORY
    notes: >-
      The lung is involved in essentially every patient and is the usual biopsy
      site.
  icdo_morphology:
    classification_value: Lymphoma
    evidence:
    - reference: PMID:32107539
      reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
      supports: SUPPORT
      evidence_source: OTHER
      snippet: "Lymphomatoid granulomatosis (LYG) is a rare Epstein-Barr virus (EBV)-driven B-cell lymphoproliferative disease (LPD)."
      explanation: >-
        Places LYG in the B-cell lymphoproliferative family, supporting the
        ICD-O lymphoma morphology bucket.
infectious_agent:
- name: Epstein-Barr virus
  description: >-
    EBV is present in the atypical B cells in essentially every case and is
    treated as the transforming agent rather than a bystander. Patients have
    serologic evidence of past exposure but not of acute infection, and the
    measured viral load is low - the abnormality is control of a common latent
    infection, not an unusual exposure.
  infectious_agent_term:
    preferred_term: Epstein-Barr virus
    term:
      id: NCBITaxon:10376
      label: human gammaherpesvirus 4
  evidence:
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "All patients had past EBV exposure by serology but with a low median EBV viral load."
    explanation: >-
      Shows that the relevant abnormality is EBV control rather than an acute or
      high-burden infection.
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "Lymphomatoid granulomatosis (LYG) is a rare Epstein-Barr virus (EBV)-driven B-cell lymphoproliferative disease (LPD). This disease is hypothesized to result from defective immune surveillance of EBV, with most patients showing evidence of immune dysfunction, despite no known primary immunodeficiency."
    explanation: >-
      States both the causal role of EBV and the immune-surveillance model this
      entry is built around.
has_subtypes:
- name: Grade 1
  display_name: Grade 1 (low grade)
  description: >-
    Polymorphous infiltrate with rare atypical medium-to-large lymphoid cells and
    a paucity of EBV-positive cells, fewer than five per high-power field.
    Necrosis is focal or absent and the infiltrate is usually polyclonal - only
    about 8% of grade 1 lesions carry a clonal immunoglobulin rearrangement. This
    is the grade treated as immune-dependent, and the grade hardest to diagnose
    because the EBV-positive cells can be sparse or absent.
  subtype_term:
    preferred_term: grade I lymphomatoid granulomatosis
    term:
      id: MONDO:0859747
      label: grade I lymphomatoid granulomatosis
  evidence:
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Immunoglobulin gene rearrangement studies were performed, and a higher percentage of clonal rearrangements were seen in LYG grade 2 (50%) and grade 3 (69%) as compared with grade 1 (8%)."
    explanation: >-
      Gives the grade 1 clonality figure that distinguishes it from the higher
      grades.
- name: Grade 2
  display_name: Grade 2 (low grade)
  description: >-
    More numerous large atypical cells, sometimes reminiscent of
    Hodgkin/Reed-Sternberg cells, with near-universal EBER positivity at five to
    twenty cells per high-power field and more necrosis than grade 1. Half of
    grade 2 lesions are clonal. Grouped with grade 1 as low-grade,
    immune-dependent disease for treatment purposes.
  subtype_term:
    preferred_term: grade II lymphomatoid granulomatosis
    term:
      id: MONDO:0859748
      label: grade II lymphomatoid granulomatosis
  evidence:
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "In accordance with the World Health Organization classification, grade 1 lesions were associated with rare atypical medium-to-large lymphoid cells, whereas a greater number of large atypical cells, occasionally reminiscent of Hodgkin/Reed-Sternberg cells, were frequently seen in grade 2 and 3 lesions."
    explanation: >-
      Describes the cytologic difference between grade 1 and the higher grades.
- name: Grade 3
  display_name: Grade 3 (high grade)
  description: >-
    Sheets of large atypical EBV-positive B cells, more than fifty and often more
    than a hundred per high-power field, with extensive coagulative necrosis.
    About 69% are clonal. This grade behaves as, and is treated as, an aggressive
    EBV-positive large B-cell lymphoma - immune-independent disease.
  subtype_term:
    preferred_term: grade III lymphomatoid granulomatosis
    term:
      id: MONDO:0859749
      label: grade III lymphomatoid granulomatosis
  evidence:
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "In contrast, near universal expression of EBER was seen in grade 2 (5-20 and occasionally up to 50 cells per high-power field) and grade 3 (>50 and often >100 cells per high power field) lesions"
    explanation: >-
      Gives the EBER density thresholds that define grade 2 and grade 3.
prevalence:
- population: Worldwide
  measure_type: UNKNOWN
  prevalence_class: ULTRA_RARE
  notes: >-
    No incidence or prevalence estimate has been published. Cohort size is the
    only available proxy: the largest single-institution pathology series covers
    55 patients referred to one national centre over 15 years, and the pivotal
    prospective trial accrued 67 patients over 28 years.
  evidence:
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "We reviewed the biopsies of 55 patients with LYG who were referred for a prospective trial at the National Cancer Institute (1995 to 2010) and evaluated the histologic, immunohistochemical, in situ hybridization, and molecular findings of these biopsies in conjunction with clinical information."
    explanation: >-
      Establishes the scale of the largest available series, which is the only
      quantitative handle on how rare the disease is.
progression:
- phase: Untreated or steroid-treated natural history
  notes: >-
    Historically poor. Before grade-stratified therapy, patients treated with
    steroids and/or chemotherapy had a median survival of about 14 months, and
    median overall survival across the disease was under two years.
  evidence:
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "In regards to treatment, historical outcomes of patients treated with steroids and/or chemotherapy have been poor (median survivals of 14 months)"
    explanation: >-
      Gives the historical median survival figure for the pre-stratification era.
  - reference: PMID:37011643
    reference_title: "Interferon alfa-2b in patients with low-grade lymphomatoid granulomatosis and chemotherapy with DA-EPOCH-R in patients with high-grade lymphomatoid granulomatosis: an open-label, single-centre, phase 2 trial."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Lymphomatoid granulomatosis is a rare Epstein-Barr virus-associated B-cell lymphoproliferative disorder with a median overall survival of less than 2 years."
    explanation: >-
      The trial's own statement of the survival baseline it set out to improve.
- phase: Grade transformation in both directions
  notes: >-
    Grade is not a fixed property of a patient. Low-grade disease can progress to
    high-grade disease after immune modulation, and high-grade disease can recur
    as low-grade disease after immunochemotherapy. Both directions are managed by
    crossing over to the other arm of therapy, which is a direct clinical
    consequence of the shared underlying defect in EBV surveillance.
  evidence:
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "Given the underlying defective immune surveillance of EBV, patients with high-grade disease may have a recurrence in the form of low-grade disease after immunochemotherapy, and those with low-grade disease may progress to high-grade disease after immune modulation, which can be effectively managed with crossover treatment."
    explanation: >-
      States the bidirectional grade shift and its mechanistic explanation.
- phase: Progression to overt EBV-positive lymphoma or organ failure
  notes: >-
    Without recognition and grade-appropriate treatment the disease can progress
    to pulmonary failure, central nervous system disease, or frank EBV-positive
    lymphoma.
  evidence:
  - reference: PMID:23006954
    reference_title: "Lymphomatoid granulomatosis."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "Lymphomatoid granulomatosis can lead to progressive pulmonary failure, central nervous system disease, or progression to overt EBV-positive lymphoma without appropriate recognition and management."
    explanation: >-
      Names the three endpoints of untreated progression.
pathophysiology:
- name: Defective EBV Immunosurveillance
  biological_scale: ORGANISM
  conforms_to: "viral_oncogenesis#Persistent Oncogenic Virus Infection"
  description: >-
    Latent EBV, carried by most of the adult population, escapes control in a
    host whose cell-mediated surveillance of EBV-infected B cells is
    quantitatively or qualitatively impaired. Every patient treated at the
    reporting national centre had moderately to severely reduced circulating
    CD3-positive T cells with the deficit falling more heavily on the
    CD8-positive compartment, and none had a recognised primary immunodeficiency
    - so the defect is inferred to be pre-existing and subtle rather than
    syndromic. Recognised syndromic and iatrogenic causes of the same defect
    exist and predispose to the disease.
  cell_types:
  - preferred_term: CD8-positive, alpha-beta T cell
    term:
      id: CL:0000625
      label: CD8-positive, alpha-beta T cell
  biological_processes:
  - preferred_term: viral latency
    modifier: INCREASED
    term:
      id: GO:0019042
      label: viral latency
  - preferred_term: T cell mediated immunity
    modifier: DECREASED
    term:
      id: GO:0002456
      label: T cell mediated immunity
  evidence:
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Taken together, these results suggest that the immunologic deficits are likely preexistent and that a quantitative and/or qualitative defect in mainly CD8+cytotoxic T cells may be a prerequisite for disease."
    explanation: >-
      States the CD8-centred surveillance defect and its position upstream of
      disease.
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "More recently, it has been hypothesized that patients with LYG have defective immune surveillance of EBV-infected B cells particularly by the CD8-positive T cells"
    explanation: >-
      Independent statement of the same model from the pathology series.
  downstream:
  - target: Outgrowth of EBV-Positive Atypical B Cells
    description: >-
      Loss of cytotoxic control permits EBV-infected B cells to expand rather
      than be cleared.
- name: Outgrowth of EBV-Positive Atypical B Cells
  biological_scale: CELLULAR
  description: >-
    EBV-infected B cells expressing latent viral proteins proliferate and
    accumulate in extranodal tissue. Their number and density per high-power
    field is precisely what histologic grade measures, so this node is the one
    the grading system quantifies. Nodal and bone marrow compartments are spared,
    which is one of the features that separates the disease from other
    EBV-positive B-cell lymphoproliferations.
  cell_types:
  - preferred_term: EBV-positive atypical B cell
    term:
      id: CL:0000236
      label: B cell
  biological_processes:
  - preferred_term: cell population proliferation
    modifier: INCREASED
    term:
      id: GO:0008283
      label: cell population proliferation
  locations:
  - preferred_term: lung
    term:
      id: UBERON:0002048
      label: lung
  evidence:
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "Pathologically, LYG is graded by the number and density of EBV+ atypical B cells, and other characteristic findings include an angioinvasive/angiodestructive reactive T-cell infiltrate and various degrees of necrosis."
    explanation: >-
      Identifies the EBV-positive atypical B-cell population as the graded
      quantity.
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "Clinically, LYG universally involves the lungs with other common extranodal sites, including skin, central nervous system, liver, and kidneys. Nodal and/or bone marrow involvement is extremely rare and, if present, suggests an alternative diagnosis."
    explanation: >-
      Establishes the extranodal distribution and the diagnostic weight of nodal
      sparing.
  downstream:
  - target: Ineffective Angiocentric Reactive T-cell Response
    description: >-
      The expanding EBV-positive population recruits a large but ineffective
      reactive T-cell infiltrate.
  - target: Clonal Selection and High-Grade Transformation
    description: >-
      Continued proliferation under persistent immune failure selects for
      transformed clones.
- name: Ineffective Angiocentric Reactive T-cell Response
  biological_scale: TISSUE
  description: >-
    A dense reactive T-cell infiltrate, CD4-predominant rather than CD8, gathers
    around and invades vessel walls without clearing the EBV-positive B cells.
    The interferon-inducible chemokines CXCL9 and CXCL10 localise to reactive
    cells in viable tissue at the edge of necrotic areas rather than to the
    malignant cells, which is the basis for attributing the vascular damage to
    the host response rather than to the tumour. The vascular injury is
    angiocentric and angiodestructive but is not a primary vasculitis - the
    vessel wall is damaged by lymphocytic invasion, not by an immune-mediated
    attack on the wall itself.
  cell_types:
  - preferred_term: CD4-positive, alpha-beta T cell
    term:
      id: CL:0000624
      label: CD4-positive, alpha-beta T cell
  biological_processes:
  - preferred_term: chemokine-mediated signaling pathway
    modifier: INCREASED
    term:
      id: GO:0070098
      label: chemokine-mediated signaling pathway
  - preferred_term: inflammatory response
    modifier: INCREASED
    term:
      id: GO:0006954
      label: inflammatory response
  locations:
  - preferred_term: blood vessel
    term:
      id: UBERON:0001981
      label: blood vessel
  evidence:
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "CXCL9 and CXCL10 localize to the reactive cells in the viable tissue surrounding areas of necrosis, suggesting that cells in the tumor microenvironment and not the malignant cells themselves are the principal source of chemokines, resulting in vascular damage and necrosis."
    explanation: >-
      Locates the chemokine source in the reactive microenvironment, which is
      what makes this node distinct from the B-cell node upstream of it.
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "Thus, the host immune response to EBV is a principal cause of the vasculitic changes that are such a typical feature of the disease."
    explanation: >-
      States directly that the host response, not the neoplastic clone, drives
      the vascular pathology.
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "Most of the angiocentric lymphocytes are CD3+T cells, with CD4+cells representing the predominant subtype in most cases."
    explanation: >-
      Establishes the CD4-predominant composition of the angiocentric infiltrate.
  downstream:
  - target: Coagulative Necrosis and Multi-organ Tissue Destruction
    description: >-
      Vascular invasion and destruction produce ischaemic coagulative necrosis in
      the involved tissue.
- name: Coagulative Necrosis and Multi-organ Tissue Destruction
  biological_scale: TISSUE
  description: >-
    Coagulative necrosis follows the vascular damage and is present at every
    grade, focal in grade 1 and often extensive in grades 2 and 3. In the lung
    this produces the bilateral nodules, sometimes cavitating, that define the
    radiologic picture; in skin, central nervous system, liver and kidney it
    produces the corresponding extranodal lesions.
  biological_processes:
  - preferred_term: cell death
    modifier: INCREASED
    term:
      id: GO:0008219
      label: cell death
  locations:
  - preferred_term: lung
    term:
      id: UBERON:0002048
      label: lung
  - preferred_term: brain
    term:
      id: UBERON:0000955
      label: brain
  - preferred_term: skin of body
    term:
      id: UBERON:0002097
      label: skin of body
  - preferred_term: kidney
    term:
      id: UBERON:0002113
      label: kidney
  - preferred_term: liver
    term:
      id: UBERON:0002107
      label: liver
  evidence:
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Necrosis was seen in all grades, with a greater degree in high-grade lesions."
    explanation: >-
      Establishes necrosis as a feature of every grade with a grade-dependent
      extent.
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "We reviewed 122 biopsies; the most common site was lung (73%), followed by skin/subcutaneous tissue (17%); other sites included kidney, nasal cavity, gastrointestinal tract, conjunctiva, liver, and adrenal gland."
    explanation: >-
      Documents the organ distribution of biopsy-proven tissue destruction.
  downstream:
  - target: Bilateral Pulmonary Nodules
    description: >-
      Necrotic angiocentric lesions in the lung are what imaging shows as
      bilateral nodules.
- name: Clonal Selection and High-Grade Transformation
  biological_scale: CELLULAR
  description: >-
    With continued proliferation under a persistent surveillance defect, the
    EBV-infected B-cell population shifts from polyclonal to monoclonal.
    Immunoglobulin gene rearrangement is clonal in 8% of grade 1, 50% of grade 2
    and 69% of grade 3 lesions - a monotonic gradient that is the molecular
    reading of the grading system. At grade 3 the lesion is no longer separable
    from an EBV-positive large B-cell lymphoma, which is why the treatment
    strategy switches from restoring immunity to killing the clone. No recurrent
    chromosomal abnormality has been found, so this transformation is not
    anchored to a known cytogenetic driver.
  cell_types:
  - preferred_term: EBV-positive atypical B cell
    term:
      id: CL:0000236
      label: B cell
  biological_processes:
  - preferred_term: cell population proliferation
    modifier: INCREASED
    term:
      id: GO:0008283
      label: cell population proliferation
  mechanism_confidence: PROVISIONAL
  evidence:
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Immunoglobulin gene rearrangement studies were performed, and a higher percentage of clonal rearrangements were seen in LYG grade 2 (50%) and grade 3 (69%) as compared with grade 1 (8%)."
    explanation: >-
      Provides the grade-clonality gradient that this node describes.
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "This shift in clonality from polyclonal to monoclonal disease seen with increased histologic grade most likely represents the selective transformation of EBV-infected B-cell clones"
    explanation: >-
      States the clonal-selection interpretation of that gradient, hedged by its
      authors as the likely explanation, which is why this node is provisional.
phenotypes:
- category: Respiratory
  name: Bilateral Pulmonary Nodules
  description: >-
    Bilateral pulmonary nodules or masses, often peribronchovascular and
    lower-lobe predominant and sometimes cavitating. Lung involvement is
    essentially universal, and its absence should prompt reconsideration of the
    diagnosis.
  phenotype_term:
    preferred_term: Pulmonary nodule
    term:
      id: HP:0033608
      label: Pulmonary nodule
  frequency: VERY_FREQUENT
  diagnostic: true
  evidence:
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Clinically, all patients had lung involvement (100%), with the next most common site being the central nervous system (38%). No patient had nodal or bone marrow disease."
    explanation: >-
      Gives the 100% lung involvement figure in the largest pathology series.
  - reference: PMID:23006954
    reference_title: "Lymphomatoid granulomatosis."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "It is usually a progressive disorder that virtually always involves the lung and characteristically presents as bilateral pulmonary nodules."
    explanation: >-
      Confirms bilateral nodules as the characteristic pulmonary presentation.
- category: Nervous System
  name: Central Nervous System Involvement
  description: >-
    Parenchymal brain lesions, with cranial neuropathies, seizures and focal
    deficits as their clinical expression. The central nervous system is the
    second most common site of disease after the lung and is an adverse
    prognostic feature.
  review_notes: >-
    The bound term covers the structural lesion, which is what the cited cohort
    counted. The individual neurologic symptoms are not separately enumerated in
    that source, so no narrower term is used.
  phenotype_term:
    preferred_term: Morphological central nervous system abnormality
    term:
      id: HP:0002011
      label: Morphological central nervous system abnormality
  frequency: FREQUENT
  evidence:
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Clinically, all patients had lung involvement (100%), with the next most common site being the central nervous system (38%). No patient had nodal or bone marrow disease."
    explanation: >-
      Gives the 38% frequency of central nervous system involvement supporting
      the FREQUENT band.
- category: Integument
  name: Cutaneous Nodules and Plaques
  description: >-
    Dermal papules and subcutaneous nodules, plaques or a patchy erythematous
    rash, present in roughly a sixth of biopsied sites. Cutaneous lesions show a
    dense lymphohistiocytic infiltrate with nonnecrotizing granulomatous
    inflammation and only sparse EBV-positive cells, so skin lesions alone cannot
    establish the diagnosis.
  review_notes: >-
    No frequency is recorded. The available figure, 17%, is a share of 122
    biopsies rather than of patients, and the FrequencyEnum bands are defined
    over patients.
  phenotype_term:
    preferred_term: Skin nodule
    term:
      id: HP:0200036
      label: Skin nodule
  diagnostic: false
  evidence:
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "We reviewed 122 biopsies; the most common site was lung (73%), followed by skin/subcutaneous tissue (17%); other sites included kidney, nasal cavity, gastrointestinal tract, conjunctiva, liver, and adrenal gland."
    explanation: >-
      Gives skin and subcutaneous tissue as the second most frequently biopsied
      site.
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "For this reason, skin lesions alone are inadequate to establish a definitive diagnosis of LYG."
    explanation: >-
      Supports marking the cutaneous phenotype as non-diagnostic on its own.
- category: Respiratory
  name: Dyspnea
  description: >-
    Breathlessness from progressive pulmonary infiltration; part of the
    presenting respiratory syndrome along with cough and chest pain.
  phenotype_term:
    preferred_term: Dyspnea
    term:
      id: HP:0002094
      label: Dyspnea
  evidence:
  - reference: PMID:23006954
    reference_title: "Lymphomatoid granulomatosis."
    supports: SUPPORT
    directness: INDIRECT
    evidence_source: OTHER
    snippet: "Lymphomatoid granulomatosis can lead to progressive pulmonary failure, central nervous system disease, or progression to overt EBV-positive lymphoma without appropriate recognition and management."
    explanation: >-
      Supports progressive pulmonary compromise as a disease consequence. The
      review does not enumerate individual respiratory symptoms, so breathlessness
      follows by inference and the item is marked INDIRECT.
histopathology:
- name: Angiocentric and angiodestructive polymorphous infiltrate
  description: >-
    A polymorphous lymphoid infiltrate centred on and invading vessels, rich in
    reactive T cells, containing large atypical EBV-positive B cells, with
    coagulative necrosis. This combination is the diagnostic finding and is what
    separates the disease from other EBV-positive B-cell lymphoproliferations.
  diagnostic: true
  evidence:
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Histologically, the lesions showed angiocentricity, were rich in T cells, had large atypical B cells, and were positive for EBV."
    explanation: >-
      States the four features that together define the diagnostic histology.
- name: Grade assignment by EBER-positive B-cell density
  description: >-
    Grade is assigned from the number and density of EBER-positive atypical B
    cells and the extent of coagulative necrosis, predominantly on the diagnostic
    lung biopsy. In the reference series grades were distributed 30% grade 1, 22%
    grade 2 and 48% grade 3. Grading is acknowledged to be inconsistent and
    poorly reproducible between observers, which matters because it selects the
    treatment arm.
  diagnostic: true
  evidence:
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Grading was performed predominantly on the lung biopsy at diagnosis; they were distributed as follows: LYG grade 1 (30%), grade 2 (22%), and grade 3 (48%)."
    explanation: >-
      Gives the grade distribution and confirms the lung biopsy as the grading
      substrate.
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "However, in practice, the grading can be inconsistent and poorly reproducible among observers."
    explanation: >-
      Records the reproducibility limitation of the grading system that drives
      therapy.
diagnosis:
- name: Tissue biopsy with EBER in situ hybridization
  description: >-
    Definitive diagnosis needs tissue, usually a lung biopsy, showing the
    angiocentric polymorphous infiltrate with EBER-positive atypical B cells.
    Immunohistochemistry for CD20, CD3, CD4, CD8 and LMP1 and immunoglobulin gene
    rearrangement studies support the diagnosis and set the grade.
  evidence:
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "LYG is a distinct entity that can usually be differentiated from other EBV-associated B-cell lymphoproliferative disorders on the basis of the combination of clinical presentation, histology, and EBV studies."
    explanation: >-
      States that the diagnosis rests on the combination of clinical picture,
      histology and EBV studies.
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Grading of these lesions is important because it dictates the treatment choice."
    explanation: >-
      Explains why grading, and therefore adequate tissue, is a diagnostic
      requirement rather than an academic exercise.
- name: Recognition of diagnostic delay
  description: >-
    The combination of rarity and a nonspecific presentation - cough,
    breathlessness, fever, pulmonary nodules - routinely delays diagnosis, and
    the differential includes both infection and vasculitis.
  evidence:
  - reference: PMID:23006954
    reference_title: "Lymphomatoid granulomatosis."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "The rareness of LYG in conjunction with its nonspecific presentation contributes to delays in diagnosis in many situations."
    explanation: >-
      Documents diagnostic delay as a recognised feature of the disease.
differential_diagnoses:
- name: Other EBV-positive B-cell lymphoproliferative disorders
  description: >-
    EBV-positive diffuse large B-cell lymphoma and the post-transplant
    lymphoproliferative disorders share EBV positivity and large atypical B
    cells. The separating features are the angiocentric architecture, the
    obligatory pulmonary involvement, and the absence of nodal and bone marrow
    disease.
  distinguishing_features:
  - Angiocentric and angiodestructive architecture with a reactive T-cell-rich background
  - Lung involvement in essentially every patient
  - Nodal or bone marrow involvement argues for an alternative diagnosis
  evidence:
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "Clinically, LYG universally involves the lungs with other common extranodal sites, including skin, central nervous system, liver, and kidneys. Nodal and/or bone marrow involvement is extremely rare and, if present, suggests an alternative diagnosis."
    explanation: >-
      Gives the distribution rule used to separate this disease from other
      EBV-positive B-cell lymphoproliferations.
- name: T-cell and NK-cell lymphomas of the angiocentric group
  description: >-
    Extranodal NK/T-cell lymphoma of nasal type and peripheral T-cell lymphoma
    were historically pooled with this disease under the label angiocentric
    immunoproliferative lesion, and misclassification confounded the older
    literature. On expert re-review of cases referred as possible lymphomatoid
    granulomatosis, a fifth turned out to be something else.
  distinguishing_features:
  - The neoplastic population is B-cell, not T-cell or NK-cell
  - Expert re-review reclassified 14 of 69 referred cases, including NK/T-cell lymphoma, peripheral T-cell lymphoma and classical Hodgkin lymphoma
  evidence:
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "The diagnoses on review were: NK/T cell lymphoma, nasal type (1 case), peripheral T-cell lymphoma, not otherwise specified (1 case), classical Hodgkin's lymphoma (4 cases), and EBV positive polymorphic or monomorphic B-cell lymphoma, lacking features of LYG (8 cases)."
    explanation: >-
      Enumerates what referred cases actually turned out to be, which is the
      practical content of this differential.
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Earlier studies were likely confounded by the inclusion of T and NK cell lymphomas (formerly included in the diagnosis of angiocentric immunoproliferative lesion) with cases of LYG"
    explanation: >-
      Records the historical conflation that makes this differential important
      when reading older literature.
treatments:
- name: Interferon Alfa-2b
  description: >-
    Dose-escalated subcutaneous interferon alfa-2b, from 7.5 million
    international units three times weekly and continued for up to a year past
    best response, for low-grade disease. The rationale is explicitly
    mechanistic: low-grade disease is treated as immune-dependent, so the
    intervention augments the host response to EBV rather than killing the clone.
    In the phase 2 trial it produced complete responses in 61% of evaluable
    patients and was substantially better tolerated than chemotherapy.
  therapeutic_modality: OTHER
  treatment_term:
    preferred_term: Pharmacotherapy
    term:
      id: NCIT:C15986
      label: Pharmacotherapy
    therapeutic_agent:
    - preferred_term: recombinant interferon alfa-2b
      term:
        id: NCIT:C1953
        label: Recombinant Interferon Alfa-2b
  target_mechanisms:
  - target: Defective EBV Immunosurveillance
    description: >-
      Augments the host immune response to EBV, which is the defect this node
      records.
  evidence:
  - reference: PMID:37011643
    reference_title: "Interferon alfa-2b in patients with low-grade lymphomatoid granulomatosis and chemotherapy with DA-EPOCH-R in patients with high-grade lymphomatoid granulomatosis: an open-label, single-centre, phase 2 trial."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "After initial treatment with interferon alfa-2b, the overall response was 64% (28 of 44 evaluable patients) with 61% (27 of 44) having a complete response"
    explanation: >-
      Gives the response rates from the pivotal prospective trial.
  - reference: PMID:37011643
    reference_title: "Interferon alfa-2b in patients with low-grade lymphomatoid granulomatosis and chemotherapy with DA-EPOCH-R in patients with high-grade lymphomatoid granulomatosis: an open-label, single-centre, phase 2 trial."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Interferon alfa-2b is efficacious for treating low-grade lymphomatoid granulomatosis and hence reducing progression to high-grade disease, whereas patients with high-grade lymphomatoid granulomatosis showed expected responses to chemotherapy."
    explanation: >-
      The trial's own conclusion, including the claim that treating low-grade
      disease reduces progression to high grade.
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "Methods of augmenting the immune response to EBV in low-grade LYG include treatment with interferon-α2b, whereas high-grade disease requires immunochemotherapy."
    explanation: >-
      States the mechanistic rationale that makes this an immune-directed rather
      than cytotoxic treatment.
- name: DA-EPOCH-R
  description: >-
    Six three-weekly cycles of dose-adjusted etoposide, prednisone, vincristine,
    cyclophosphamide, doxorubicin and rituximab for high-grade disease, which is
    treated as immune-independent. Complete responses were seen in 47% of
    evaluable patients. Toxicity is the trade-off: serious adverse events
    occurred in 64% of chemotherapy-treated patients against 25% of those on
    interferon alfa-2b.
  therapeutic_modality: SMALL_MOLECULE
  treatment_term:
    preferred_term: chemotherapy
    term:
      id: NCIT:C15632
      label: Chemotherapy
    therapeutic_agent:
    - preferred_term: etoposide
      term:
        id: CHEBI:4911
        label: etoposide
    - preferred_term: prednisone
      term:
        id: CHEBI:8382
        label: prednisone
    - preferred_term: vincristine
      term:
        id: CHEBI:28445
        label: vincristine
    - preferred_term: cyclophosphamide
      term:
        id: CHEBI:4027
        label: cyclophosphamide
    - preferred_term: doxorubicin
      term:
        id: CHEBI:28748
        label: doxorubicin
    - preferred_term: rituximab
      term:
        id: NCIT:C1702
        label: Rituximab
  regimen_term:
    preferred_term: dose-adjusted EPOCH-R regimen
    term:
      id: NCIT:C140097
      label: Dose-adjusted EPOCH-R Regimen
  target_mechanisms:
  - target: Clonal Selection and High-Grade Transformation
    description: >-
      Cytotoxic and anti-CD20 therapy directed at the transformed clone that
      defines high-grade disease.
  evidence:
  - reference: PMID:37011643
    reference_title: "Interferon alfa-2b in patients with low-grade lymphomatoid granulomatosis and chemotherapy with DA-EPOCH-R in patients with high-grade lymphomatoid granulomatosis: an open-label, single-centre, phase 2 trial."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "After initial treatment with DA-EPOCH-R, the overall response was 76% (13 of 17 evaluable patients) with 47% (eight of 17) having a complete response"
    explanation: >-
      Gives the response rates for the high-grade arm of the trial.
  - reference: PMID:37011643
    reference_title: "Interferon alfa-2b in patients with low-grade lymphomatoid granulomatosis and chemotherapy with DA-EPOCH-R in patients with high-grade lymphomatoid granulomatosis: an open-label, single-centre, phase 2 trial."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Serious adverse events occurred in 13 (25%) of 51 patients receiving treatment with interferon alfa-2b and 21 (64%) of 33 patients receiving DA-EPOCH-R, with five treatment-related deaths: one thromboembolic, one infection, and one haemophagocytic syndrome with interferon alfa-2b, and one infection and one haemophagocytic syndrome with DA-EPOCH-R."
    explanation: >-
      Quantifies the toxicity difference between the two arms, including
      treatment-related deaths in both.
- name: Crossover Between Treatment Arms
  description: >-
    Patients with residual or progressive disease after initial therapy cross
    over to the other arm. This is not a salvage improvisation but a designed
    consequence of the model: the underlying EBV surveillance defect persists
    through chemotherapy, so high-grade disease can recur as low-grade disease
    and be treated with interferon, and low-grade disease can transform under
    immune modulation and need chemotherapy.
  therapeutic_modality: OTHER
  treatment_term:
    preferred_term: Therapeutic Procedure
    term:
      id: NCIT:C49236
      label: Therapeutic Procedure
  evidence:
  - reference: PMID:37011643
    reference_title: "Interferon alfa-2b in patients with low-grade lymphomatoid granulomatosis and chemotherapy with DA-EPOCH-R in patients with high-grade lymphomatoid granulomatosis: an open-label, single-centre, phase 2 trial."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Patients with residual or progressive disease after initial therapy crossed over to alternative therapy."
    explanation: >-
      Documents crossover as part of the prospective trial design.
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "Given the underlying defective immune surveillance of EBV, patients with high-grade disease may have a recurrence in the form of low-grade disease after immunochemotherapy, and those with low-grade disease may progress to high-grade disease after immune modulation, which can be effectively managed with crossover treatment."
    explanation: >-
      Gives the mechanistic reason crossover is expected rather than
      exceptional.
- name: Hematopoietic Stem Cell Transplantation
  description: >-
    Considered for primary refractory disease or multiple relapses. Its efficacy
    in this disease is explicitly described as not well established, so it is
    recorded here as an option rather than a standard.
  therapeutic_modality: CELL_THERAPY
  treatment_term:
    preferred_term: Hematopoietic Cell Transplantation
    term:
      id: NCIT:C15431
      label: Hematopoietic Cell Transplantation
  evidence:
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    directness: DIRECT
    evidence_source: OTHER
    snippet: "In patients with primary refractory disease or in those with multiple relapses, hematopoietic stem cell transplantation may be considered, but its efficacy is not well established."
    explanation: >-
      Supports transplantation as a considered option while explicitly declining
      to claim established efficacy.
clinical_trials:
- name: NCT00001379
  phase: PHASE_II
  status: COMPLETED
  description: >-
    Treatment and Natural History Study of Lymphomatoid Granulomatosis. The
    single-centre trial that established grade-stratified therapy: interferon
    alfa-2b for grade 1 and 2 disease, DA-EPOCH-R for grade 3, with crossover for
    residual or progressive disease. Its results are the evidence base for the
    treatments recorded in this entry.
  target_phenotypes:
  - preferred_term: Pulmonary nodule
    term:
      id: HP:0033608
      label: Pulmonary nodule
  evidence:
  - reference: clinicaltrials:NCT00001379
    reference_title: "Treatment and Natural History Study of Lymphomatoid Granulomatosis"
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "If patients have grade 3 disease, they will usually receive etoposide, prednisone, vincristine, cyclophosphamide, and doxorubicin (EPOCH)-rituximab (EPOCH-R) chemotherapy (each letter representing a drug). If patients have grade 1 or 2 disease, they will usually receive alpha interferon."
    explanation: >-
      The trial record states the grade-stratified allocation this entry's
      treatment section is built on.
discussions:
- discussion_id: lyg_immune_defect_unidentified
  kind: KNOWLEDGE_GAP
  status: OPEN
  prompt: >-
    What is the specific immune defect that permits lymphomatoid granulomatosis
    in patients with no recognised primary immunodeficiency?
  attaches_to:
  - pathophysiology#Defective EBV Immunosurveillance
  rationale: >-
    The upstream node of this entry is a defect that has been measured only as an
    aggregate: reduced circulating T cells, falling more on the CD8 compartment,
    in patients who have no diagnosed immunodeficiency syndrome. It is inferred
    to be pre-existing and is described by its own authors as hypothesised. No
    causal gene, no functional assay and no biomarker identifies it in an
    individual patient, so the entry has a trigger node it cannot yet
    characterise molecularly.
  evidence:
  - reference: PMID:32107539
    reference_title: "Pathobiology and treatment of lymphomatoid granulomatosis, a rare EBV-driven disorder."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Taken together, these results suggest that the immunologic deficits are likely preexistent and that a quantitative and/or qualitative defect in mainly CD8+cytotoxic T cells may be a prerequisite for disease."
    explanation: >-
      The hedged language is the point: this is the strongest available
      characterisation of the defect.
- discussion_id: lyg_grading_reproducibility
  kind: KNOWLEDGE_GAP
  status: OPEN
  prompt: >-
    How should treatment be allocated when the grading that selects the treatment
    arm is not reproducible between observers?
  attaches_to:
  - histopathology#Grade assignment by EBER-positive B-cell density
  - treatments#
  rationale: >-
    Grade decides whether a patient receives interferon alfa-2b or
    chemoimmunotherapy, and the toxicity difference between those arms is large -
    serious adverse events in 25% against 64%. The pathologists who defined the
    grading system record in the same paper that it is inconsistent and poorly
    reproducible between observers, with grade 1 the hardest because the
    EBV-positive cells can be sparse or absent. No reproducibility study,
    quantitative EBER threshold or adjunct molecular assay has been published to
    close the gap.
  evidence:
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "However, in practice, the grading can be inconsistent and poorly reproducible among observers."
    explanation: >-
      States the reproducibility problem directly.
  - reference: PMID:25321327
    reference_title: "Lymphomatoid granulomatosis--a single institute experience: pathologic findings and clinical correlations."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "The greatest difficulty for the diagnosis of LYG has been with grade 1 lesions because of the possible absence or paucity of EBV-positive atypical B cells."
    explanation: >-
      Identifies grade 1 as the specific point of failure, which is also the
      grade that selects the low-toxicity arm.
- discussion_id: lyg_no_model_system
  kind: HUMAN_MODEL_MISMATCH
  status: OPEN
  prompt: >-
    Do humanized-mouse models of EBV lymphoproliferation actually model
    lymphomatoid granulomatosis, or only the generic EBV-plus-immunosuppression
    axis?
  attaches_to:
  - pathophysiology#Ineffective Angiocentric Reactive T-cell Response
  rationale: >-
    No animal or in vitro model of this disease exists. Humanized mice
    reconstituted with human haematopoietic cells develop EBV-associated B-cell
    lymphoproliferation, and immunosuppression increases its frequency, so they
    model the upstream axis. They do not reproduce what actually defines the
    disease - the angiocentric and angiodestructive architecture, the
    lung-skin-CNS distribution, or the CD4-predominant reactive microenvironment.
    The entry therefore records no animal_models, and the tissue-level node has
    no experimental system behind it. This is flagged as a model mismatch rather
    than a plain knowledge gap because evidence exists in a model system whose
    fidelity to the human tissue phenotype is the open question.
  evidence:
  - reference: PMID:25436886
    reference_title: "Humanized mouse models of epstein-barr virus infection and associated diseases."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: "Thus, many aspects of human EBV infection, including associated diseases (e.g., lymphoproliferative disease, hemophagocytic lymphohistiocytosis and erosive arthritis resembling RA), latent infection, and T-cell-mediated and humoral immune responses have been successfully reproduced in humanized mice."
    explanation: >-
      Establishes what the available models do reproduce - generic EBV-driven
      lymphoproliferation and immune control - which is the upstream axis of
      this disease and not its defining tissue phenotype.
  - reference: PMID:32251475
    reference_title: "Immunosuppressive FK506 treatment leads to more frequent EBV-associated lymphoproliferative disease in humanized mice."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: "We found that FK506 treatment of EBV-infected mice led to an elevated viral burden, more frequent tumor formation and diminished EBV-induced T cell responses, indicative of reduced EBV-specific immune control."
    explanation: >-
      Shows that the immunosuppression-plus-EBV axis is modellable, and that the
      modelled endpoint is post-transplant-style lymphoproliferation rather than
      an angiocentric, lung-predominant lesion.
notes: >-
  Entity identity was checked manually because `just preflight-dr` returned SKIP:
  MONDO records no causal gene for MONDO:0019466, so its gene-identity check
  cannot discriminate. The MONDO term's own Orphanet-sourced definition matches
  the research report on every point checked - EBV-driven, fourth to sixth
  decade, lung, skin, central nervous system and kidney, with lymph nodes and
  spleen only very rarely involved.

  Grades 1, 2 and 3 are curated as `has_subtypes` rather than as `stages`
  because MONDO models them as three distinct disease children
  (MONDO:0859747/8/9) and because they select different treatment arms rather
  than marking sequential points in one course. Grade is not fixed for a
  patient, though: it moves in both directions under treatment, which the
  `progression` section records.

  No `genetic` section is curated. Germline immunodeficiency genes - DOCK8, WAS,
  SH2D1A - are reported as predisposing conditions in case reports, but they
  cause their own syndromes, of which this disease is one manifestation among
  several; folding them in here would misrepresent them as causal genes for a
  disease that has none. No recurrent chromosomal abnormality has been
  identified either.

  Scope calls made deliberately. No `environmental:` section: the recognised
  predisposing conditions are iatrogenic immunosuppression, HIV and germline
  immunodeficiency syndromes, none of which is an ECTO-style exposure acting on
  a named mechanism node, and the one thing that would qualify - EBV - is
  curated as `infectious_agent` instead. No separate molecular or viral-latency
  node: the mechanism this disease turns on is the host's failure to control a
  latent infection that is otherwise near-universal, so latency is annotated on
  the immunosurveillance node (GO:0019042) rather than given a node of its own,
  and no LYG-specific data on EBV latent gene programs was found to support one.
  No `animal_models`: see the HUMAN_MODEL_MISMATCH discussion.

  Most of the reference cache added alongside this entry comes from the
  deep-research run's own citation-validation pass rather than from curation.
  Those files are committed because the report that cites them is committed;
  they are not evidence for anything in the entry.

  Historical caution when reading pre-2001 literature: T-cell and NK-cell
  lymphomas were pooled with this disease under the label "angiocentric
  immunoproliferative lesion", and the confounding is explicitly noted by the
  reference pathology series.
📚

References & Deep Research

Deep Research

1
Claude Code
Lymphomatoid Granulomatosis: Comprehensive Research Report
claude-haiku-4-5-20251001, claude-sonnet-5 27 citations 2026-08-29T14:50:16.275062

Lymphomatoid Granulomatosis: Comprehensive Research Report

1. Disease Information

Overview. Lymphomatoid granulomatosis (LYG) is a rare, Epstein-Barr virus (EBV)–driven, angiocentric and angiodestructive B-cell lymphoproliferative disease that arises against a background of defective host immune surveillance of EBV. First described by Averill Liebow in 1972, it is now recognized in the WHO classification as a distinct entity most closely related to a T-cell/histiocyte-rich large B-cell lymphoma variant, formally classified since 2015 within the WHO Classification of Tumours of the Lung, Pleura, Thymus and Heart, and retained in the 2016/2022 WHO classifications of mature lymphoid neoplasms (Melani et al., Blood 2020, PMID 32107539; PathologyOutlines). Histologically it is defined by an angiocentric, angioinvasive, extranodal, polymorphous lymphoid infiltrate containing variable numbers of large, atypical, EBV-positive B cells set in a dense background of reactive, predominantly CD4+ T lymphocytes, with associated coagulative ("tumor") necrosis (PMID 25321327).

Key identifiers: - MONDO: MONDO:0019466 - Orphanet: ORPHA:86869 - ICD-10-CM: C83.8 (Other non-follicular lymphoma) - ICD-O: 9766/1 - OMIM: No dedicated single-gene OMIM entry exists for sporadic LYG (it is not a classic monogenic Mendelian disorder); related immunodeficiency-associated lymphoproliferative OMIM entries exist for specific germline predisposition syndromes (e.g., OMIM #613011, Lymphoproliferative syndrome 1)

Synonyms: angiocentric immunoproliferative lesion (AIL); "LG"/"LYG"; historically grouped with (though not identical to) polymorphic reticulosis/lethal midline granuloma and angiocentric lymphoma under the older umbrella term "angiocentric immunoproliferative lesions," though modern classification distinguishes LYG (B-cell driven, EBV+, multi-organ) from nasal NK/T-cell lymphoma (formerly "polymorphic reticulosis") (GARD; PMID 7281476).

Evidence basis: Information is derived almost entirely from aggregated case series, single-institution retrospective cohorts, and one prospective NCI phase 2 interventional trial — not large-scale EHR/claims data — reflecting the disease's rarity.


2. Etiology

Primary causal driver — EBV plus host immune dysfunction. LYG is hypothesized to result from defective cell-mediated immune surveillance of EBV-infected B cells, permitting outgrowth of EBV-driven B-cell clones amid a robust but ineffective reactive T-cell response ("hyperimmune" reaction), rather than from any single germline oncogenic driver (PMID 32107539). A functional defect, primarily in CD8+ cytotoxic T-cell immunosurveillance, is hypothesized as a prerequisite; notably, the reactive infiltrate itself is CD4-predominant (CD4:CD8 ratio favoring CD4 in 95% of cases, 20/21) rather than CD8-predominant, consistent with an ineffective/dysregulated rather than absent T-cell response (PMID 25321327).

Genetic/immunologic risk factors: - Underlying primary or acquired immunodeficiency is documented in a substantial fraction of cases even without a formally diagnosed syndrome. Recognized associated conditions include: - Wiskott–Aldrich syndrome (X-linked; marked predisposition to EBV-associated lymphoma) - X-linked lymphoproliferative syndrome (XLP) - Common variable immunodeficiency (CVID) - DOCK8 deficiency — a combined immunodeficiency in the hyper-IgE spectrum; a 2017 report described EBV+ LYG as a previously unreported presentation of DOCK8 deficiency, with resolution of LYG following hematopoietic stem cell transplantation in affected relatives (PMID from Frontiers report, DOCK8/LYG) - HIV/AIDS — via CD4+ T-cell depletion and loss of EBV control - Solid organ transplantation (iatrogenic immunosuppression) — placing LYG within the broader post-transplant lymphoproliferative disease (PTLD) spectrum in transplant recipients - No recurrent chromosomal abnormalities have been reported in LYG, and no single causal driver gene/variant analogous to a classic Mendelian mutation has been established (PMID 32107539). - Sporadic disease without recognized immunodeficiency is also common — most affected adults have no formally diagnosed primary immunodeficiency syndrome, suggesting a subtler or acquired immune surveillance defect specific to EBV.

Environmental/infectious factor — EBV is central and essentially obligate. EBV (a γ-herpesvirus) genomes and gene products (EBER, LMP1) are demonstrable in the neoplastic B cells of the great majority of cases across grades (EBER positivity: ~46% in grade 1, 100% in grade 2, 94% in grade 3 lesions per PMID 25321327), supporting EBV as the direct causal/transforming agent rather than a bystander.

Protective factors: No specific genetic or environmental protective factor has been established in the literature; intact cell-mediated (particularly CD8+ cytotoxic T-lymphocyte) immunosurveillance of EBV is implicitly protective, as evidenced by disease remission following immune reconstitution (e.g., antiretroviral therapy in newly diagnosed HIV, or reduction of immunosuppression in transplant recipients) (PMC11829542).

Gene-environment interaction: The disease represents a paradigmatic gene(immune)-environment(EBV) interaction: a host with a quantitative or qualitative T-cell surveillance defect (genetic/immunodeficiency-related or acquired) fails to control EBV-driven B-cell proliferation, and progressive genetic/clonal evolution of the EBV+ B-cell population (increasing clonality with grade) leads to transformation toward overt lymphoma.


3. Phenotypes

LYG is multisystemic, with organ involvement documented in a 55-patient single-institution series (Song et al., Am J Surg Pathol 2015, PMID 25321327):

Organ Frequency HPO term (suggested)
Lung ~90–100% (near-universal) HP:0006536 (Pulmonary infiltrates) / HP:0032230 (Pulmonary nodule)
CNS 20–38% HP:0002316 (CNS neoplasm) / HP:0007281
Skin 17–55% HP:0011355 (Skin nodule) / HP:0100310 (Skin ulcer)
Liver 19–29% HP:0001392 (Abnormal liver morphology)
Kidney 15–40% HP:0000077 (Abnormality of the kidney)

Pulmonary phenotype (near-universal, ~90–100% of cases): Presenting symptoms include cough, dyspnea, chest pain, and fever; imaging shows bilateral, peribronchovascular, lower/peripheral-lung-predominant nodules or masses in 80–100% of cases, with cavitation, small thin-walled cysts, atelectasis/lobar obstruction, and occasional pneumothorax (AJR, PMID 11044036).

Cutaneous phenotype (~17–55%; second most common site): Multiple erythematous dermal papules and/or subcutaneous nodules, plaques, or a patchy erythematous rash, with ulceration in up to ~30% of affected patients; distribution favors extremities over head/neck (only ~10% head/neck) (Dermatology Advisor; PMC6110445).

CNS phenotype (20–38%): Parenchymal brain lesions, cranial neuropathies, seizures, and focal neurologic deficits; CT shows high-density lesions. CNS involvement is a major adverse prognostic factor — one CNS-focused cohort reported overall mortality of 63.5% in LYG generally versus 86.0% in CNS-LYG specifically, with 5-year mortality of 38–88% and median survival 14–72 months in CNS-involved disease (PMC7516720).

Hepatic and renal phenotype: Often asymptomatic or detected on imaging/labs; renal involvement can present with hematuria or renal impairment without overt vasculitic glomerulonephritis (distinguishing it from ANCA-associated vasculitis).

Constitutional/laboratory phenotypes: Fever, weight loss, malaise; laboratory abnormalities may include cytopenias, and rare cases present with hemophagocytic lymphohistiocytosis (HLH) as an initial manifestation (Frontiers, PMC) or paraneoplastic polymyositis (PMC4757691).

Onset/course: Typically insidious onset in adults (fourth–sixth decade), though a chronic, indolent ("smoldering") cutaneous-only course has also been reported (PMC8841505); grade generally correlates with disease pace, with low-grade disease often smoldering/relapsing-remitting and high-grade disease behaving as an aggressive lymphoma.

Quality of life impact: Not systematically studied via standardized instruments (EQ-5D/SF-36) in this rare disease; qualitatively, pulmonary and CNS involvement drive the greatest functional morbidity.


4. Genetic/Molecular Information

Causal genes: No single germline causal gene for sporadic LYG exists; it is not a classic monogenic disorder. However, LYG has been reported as a rare secondary manifestation of several germline primary immunodeficiency genes: - DOCK8 (hgnc:19191) — autosomal recessive combined immunodeficiency (hyper-IgE syndrome spectrum); reported cause of EBV+ LYG with intrafamilial phenotypic variation (PMC5328973) - WAS — Wiskott-Aldrich syndrome gene, X-linked - Genes underlying XLP (SH2D1A, XIAP) and CVID

Somatic/molecular features of the neoplastic clone: - Clonality: Immunoglobulin heavy-chain gene rearrangement studies show grade-dependent clonality — grade 1: ~8% clonal (1/12); grade 2: ~50% clonal (4/8); grade 3: ~69% clonal (11/16) — consistent with progressive selection/transformation of an EBV-infected B-cell clone with increasing grade (Song et al. 2015, PMID 25321327). - No recurrent cytogenetic abnormalities (translocations, aneuploidy) have been established, unlike most other B-cell lymphomas (PMID 32107539). - EBV gene expression: Neoplastic B cells express EBER (EBV-encoded small RNA, detected by in situ hybridization) and latent membrane protein 1 (LMP1) by immunohistochemistry, consistent with a latency II/III-like expression program; EBER positivity rises from ~46% (grade 1) to 100% (grade 2) to 94% (grade 3) (PMID 25321327). - Immunophenotype of neoplastic cells: CD20+, CD45+, LMP1+ large atypical B cells; background reactive infiltrate is CD3+ T cells, CD4-predominant in 95% of cases (20/21) (PMID 25321327).

Variant classification/allele frequency: Not applicable in the classic ClinVar/gnomAD sense for sporadic LYG, since it is a somatic/EBV-driven lymphoproliferation rather than a germline variant-caused disease; where associated germline immunodeficiency genes (DOCK8, WAS) are implicated, standard ACMG/AMP pathogenic-variant classification applies to those underlying conditions rather than to LYG itself.

Epigenetics: No LYG-specific DNA methylation/histone-modification studies were identified in the search; EBV latency programs themselves involve epigenetic (CpG methylation, histone) regulation of the viral genome, a mechanism general to EBV-associated lymphoproliferations.


5. Environmental Information

Infectious trigger — EBV (Epstein-Barr virus, human gammaherpesvirus 4; NCBITaxon:10376): The central and essentially obligate etiologic agent; EBV genomes are detectable by PCR/in situ hybridization in the overwhelming majority of cases (PMID 2170969).

Iatrogenic/exposure factors: - Immunosuppressive medication — solid organ transplantation, TNF-α inhibitor therapy (a 2023 case report describes pulmonary LYG in a patient on long-term TNF-α inhibitor use, PMID 37160375), and other immunosuppressive regimens for autoimmune disease can precipitate LYG by impairing EBV surveillance. - HIV infection — a well-documented environmental/infectious risk factor via CD4+ T-cell depletion; case reports document LYG remission after immune reconstitution with antiretroviral therapy.

Lifestyle factors: No specific lifestyle risk factor (smoking, diet, alcohol) has been established in the literature as a driver of LYG.


6. Mechanism / Pathophysiology

Causal chain (upstream → downstream): 1. Trigger: EBV infection of B lymphocytes (nearly universal in the population; EBV seroprevalence >90% in adults) combined with a host defect in cell-mediated EBV immunosurveillance (constitutional immunodeficiency, HIV, iatrogenic immunosuppression, or an unidentified subtler defect). 2. Molecular/cellular consequence: Failure of cytotoxic (largely CD8+) T-cell control permits outgrowth of EBV-infected B cells expressing latent viral oncoproteins (LMP1, EBNA), which drive B-cell proliferation and survival signaling (NF-κB activation downstream of LMP1, analogous to other EBV+ lymphoproliferations). 3. Tissue-level consequence: A robust reactive, predominantly CD4+ T-cell response is recruited but is immunologically ineffective at eliminating the EBV+ B-cell population; the mixed infiltrate shows a striking tropism for blood vessel walls (angiocentricity) and vessel destruction (angioinvasion/angiodestruction), producing ischemic coagulative ("tumor") necrosis in affected tissue. 4. Organism-level consequence: Multi-organ tissue destruction (lung nodules/cavitation, cutaneous ulceration, CNS lesions, hepatic/renal involvement) and, with clonal evolution/selection of the EBV+ B-cell population (rising Ig-clonality across grades 1→3), progression from a polyclonal/oligoclonal low-grade lymphoproliferation to a clonal, aggressive B-cell lymphoma indistinguishable from EBV+ diffuse large B-cell lymphoma at grade 3.

Cellular processes involved: Chronic inflammation, angiocentric/angiodestructive vasculopathy (not a true vasculitis, since there is no primary destruction of the vessel wall by an immune-mediated vasculitic process — vessel damage is secondary to lymphocytic infiltration), ischemic/coagulative necrosis, and B-cell clonal selection/malignant transformation.

Grading as a mechanistic readout: The three-tier histologic grading system directly operationalizes the pathobiology — grade is defined by the number/density of large EBV+ B cells and extent of necrosis: - Grade 1: Polymorphous infiltrate, few/no large atypical cells, scant EBV+ B cells (EBER+ ~46%), no or minimal necrosis, low/no clonality (8% clonal) — indolent, "immune-dependent" biology. - Grade 2: Increased large EBV+ B cells (EBER+ 100%), more necrosis, intermediate clonality (50%). - Grade 3: Sheets of large atypical EBV+ B cells resembling conventional EBV+ diffuse large B-cell lymphoma, extensive necrosis, high clonality (69%) — "immune-independent," frankly malignant biology (PMID 25321327; Nakamura/Nature Modern Pathology review).

This grade-dependent biology is the direct rationale for the NCI's differentiated treatment strategy (immunotherapy for immune-dependent low-grade disease vs. cytotoxic chemoimmunotherapy for immune-independent high-grade disease — see Treatment section).

Suggested GO terms: GO:0006955 (immune response), GO:0002432 (granuloma formation), GO:0031295 (T cell costimulation) [context], GO:0043065 (positive regulation of apoptotic process) [reactive T cell attack on infected cells], GO:0001525 (angiogenesis)/GO:0032102 (negative regulation of response to wounding) not directly established. Suggested CL terms: CL:0000236 (B cell) — specifically EBV-transformed large B cell; CL:0000624 (CD4-positive, alpha-beta T cell) for the reactive infiltrate; CL:0000625 (CD8-positive, alpha-beta T cell) for the hypothesized deficient surveillance population.

Advanced/omics profiling: No large-scale transcriptomic, proteomic, single-cell, or spatial transcriptomic dataset specific to LYG was identified in this search — consistent with its rarity and the field's reliance on immunohistochemistry/ISH-based diagnostic pathology rather than genomic profiling to date. This represents a knowledge gap relative to better-characterized B-cell lymphomas.


7. Anatomical Structures Affected

  • Primary organ: Lung (near-universal, ~90–100%) — UBERON:0002048 (lung); peribronchovascular/perivascular distribution.
  • Secondary/frequently involved organs: Skin (UBERON:0002097, integument), CNS/brain (UBERON:0000955), liver (UBERON:0002107), kidney (UBERON:0002113).
  • Body systems: Respiratory, integumentary, nervous, hepatobiliary, renal — a genuinely multisystem disease; lymph nodes and spleen may be secondarily involved (splenomegaly reported, PMC6000673) but are not primary sites (an important distinguishing feature from typical nodal lymphomas).
  • Tissue level: Perivascular/angiocentric lymphoid infiltrate within pulmonary interstitium, dermis/subcutis, cerebral parenchyma; vascular smooth muscle and endothelium (targets of angioinvasion).
  • Cell populations: Large atypical EBV+ B cells (CD20+, CD45+, LMP1+, EBER+) — Cell Ontology CL:0000236 specialization; reactive CD4+ T cells (CL:0000624) as the dominant background population; histiocytes/plasma cells as minor infiltrate components.
  • Subcellular: No specific organelle-level pathology reported (this is not a classic metabolic/storage disease); relevant GO Cellular Component context would be nuclear (EBER, an RNA Pol III transcript, localizes to the nucleus) for viral gene products.
  • Localization/laterality: Pulmonary disease is characteristically bilateral; skin lesions favor extremities (bilateral, non-lateralized); CNS lesions can be single or multifocal, no strict laterality pattern reported.

8. Temporal Development

  • Onset: Typically adult-onset, fourth to sixth decade of life, though reported across the age spectrum including pediatric cases (children with LYG have been described, including as a rare complication after chemotherapy for pediatric AML, PMID 12571471, and as a cerebellar-mass presentation, AJNR 2007).
  • Onset pattern: Usually insidious/subacute; can present acutely in the context of superimposed immunosuppression (e.g., post-transplant, HIV seroconversion).
  • Staging/grading as a temporal-progression proxy: Grade 1↔2↔3 functions partly as a progression axis — low-grade disease can progress to high-grade disease over time if untreated or under continued immune impairment, motivating the rationale for early immunotherapy in low-grade disease to forestall transformation (Lancet Haematol 2023, PMID 37011643).
  • Progression rate/course pattern: Variable — ranges from an indolent, smoldering, relapsing-remitting cutaneous-limited course (PMC8841505) to a rapidly progressive, aggressive high-grade lymphoma course.
  • Remission patterns: Both spontaneous remission (rare, reported with immune reconstitution, e.g., post-antiretroviral therapy in HIV) and treatment-induced remission (interferon-alfa-2b for low-grade disease; DA-EPOCH-R for high-grade) are documented.
  • Historical natural history (untreated/steroid-treated): Median survival ~14 months, with 5-year mortality ~50% in older literature predating modern grade-stratified therapy.

9. Inheritance and Population

Epidemiology: - LYG is an exceedingly rare disease of unknown precise population prevalence/incidence — described as "a disease of unknown prevalence" with no dedicated national registry figures identified. - Sex ratio: Male predominance, approximately 2:1 male:female. - Age distribution: Most common in the fourth to sixth decades of adult life; can occur at any age, including rare pediatric cases. - Race/ethnicity: No known racial predilection reported.

Inheritance pattern: LYG itself is not inherited in a classic Mendelian sense — it is a sporadic, EBV-driven lymphoproliferation. However, when it arises secondary to a germline primary immunodeficiency (e.g., DOCK8 deficiency — autosomal recessive; Wiskott-Aldrich syndrome — X-linked recessive), the underlying predisposing condition follows that syndrome's inheritance pattern, and intrafamilial variation in LYG presentation among relatives sharing the same germline DOCK8 mutation has been documented (PMC5328973).

Penetrance/expressivity: Not classically applicable, given the sporadic/acquired nature of most cases; where a germline immunodeficiency is causal, penetrance for LYG specifically (versus other EBV-driven manifestations) is incomplete and variable even within families.

Founder effects/consanguinity/carrier frequency: Not established for LYG itself, though relevant to the rare recessive immunodeficiency syndromes (e.g., DOCK8 deficiency) that can predispose to it.


10. Diagnostics

Tissue diagnosis is required and central. Definitive diagnosis requires tissue biopsy (open lung biopsy or video-assisted thoracoscopic surgery [VATS] for pulmonary disease, or biopsy of an accessible extrapulmonary site such as skin) demonstrating the characteristic angiocentric/angioinvasive polymorphous infiltrate with EBV+ atypical B cells (emedicine Workup).

Ancillary pathology studies: - In situ hybridization for EBER (EBV-encoded RNA) — the key diagnostic test confirming EBV positivity in the atypical B-cell population. - Immunohistochemistry: CD20 (neoplastic B cells), CD3/CD4/CD8 (background reactive T cells, CD4-predominant), LMP1 (latent EBV protein). - Molecular clonality studies: Immunoglobulin heavy-chain gene rearrangement (PCR-based) to assess clonality, correlating with grade.

Imaging: - Chest CT — bilateral, peribronchovascular, lower/peripheral-lung nodules or masses, sometimes with cavitation; the primary modality for detecting and monitoring pulmonary disease. - Brain MRI/CT — for suspected CNS involvement; CT shows high-density lesions. - F18-FDG PET/CT — used to assess multisystem disease distribution, guide high-yield biopsy site selection, and monitor treatment response.

Laboratory studies: No LYG-specific serum biomarker exists; EBV serology/PCR (plasma EBV DNA load) may support the diagnosis and can be used for monitoring, though it is not diagnostic in isolation given high background EBV seroprevalence.

Genetic testing: Not part of routine LYG diagnostic workup unless an underlying primary immunodeficiency is clinically suspected (e.g., recurrent infections, eczema, and elevated IgE suggesting DOCK8 deficiency; recurrent infections/thrombocytopenia/eczema suggesting Wiskott-Aldrich syndrome), in which case targeted gene panel or exome sequencing for primary immunodeficiency genes would be pursued.

Differential diagnosis: - Granulomatosis with polyangiitis (GPA/Wegener's) — distinguished by true necrotizing vasculitis (destruction of the vessel wall itself by the inflammatory process) and typically ANCA positivity, versus LYG's angiocentric/angioinvasive-but-not-classically-vasculitic pattern and EBV positivity. - Sarcoidosis / necrotizing sarcoid granulomatosis — distinguished by well-formed granulomas with giant cells, more frequent mediastinal adenopathy, and absence of EBV+ atypical B cells (LYG characteristically lacks well-formed granulomas or multinucleated giant cells). - Other entities in the differential: pseudolymphoma, other malignant lymphomas (including EBV+ diffuse large B-cell lymphoma, into which grade 3 LYG merges diagnostically), lymphocytic interstitial pneumonia, metastatic disease, cryptogenic organizing pneumonia, infectious granulomatous disease (fungal, mycobacterial).

Screening: No population-level screening program exists given the disease's rarity and lack of an identifiable pre-symptomatic screening marker; surveillance in known immunodeficiency syndromes (DOCK8 deficiency, Wiskott-Aldrich) for EBV-driven lymphoproliferative complications is a reasonable clinical practice, though not formally codified as LYG-specific screening.


11. Outcome/Prognosis

Historical (pre-risk-stratified-therapy) outcomes: Median overall survival was poor, historically cited as 14 months (steroid/chemotherapy era) to under 2 years, with 5-year mortality around 50%.

Modern, grade-stratified therapy outcomes (NCI phase 2 trial, Lancet Haematology 2023, PMID 37011643): - Patients with low-grade disease treated with interferon alfa-2b achieved a median overall survival of approximately 20 years — a dramatic improvement over historical controls. - Progression-free survival for grades 1–2 treated with interferon-alfa was 56%, with median PFS of 5.1 years; for grade 3 treated with DA-EPOCH-R, PFS was 44%, median 32 months (PMID 25321327 reporting NCI cohort outcomes). - Complete response rates in the phase 2 trial: 61% (27/44) after initial interferon alfa-2b in low-grade disease; 47% (8/17) after initial DA-EPOCH-R in high-grade disease; with additional responses after cross-over treatment. - Serious adverse events occurred in about 25% of interferon alfa-2b-treated patients versus nearly two-thirds of chemotherapy-treated patients, favoring the tolerability of immunotherapy for low-grade disease.

Prognostic factors: - Histologic grade is the single most important prognostic factor — grade 3 (high-grade) disease behaves as an aggressive lymphoma with poorer outcomes than low-grade disease, though modern chemoimmunotherapy has substantially improved even high-grade outcomes. - CNS involvement confers markedly worse prognosis: one cohort reported 3-year overall mortality of 63.5% in LYG broadly versus 86.0% specifically in CNS-involved LYG, with 5-year mortality of 38–88% and median survival 14–72 months in CNS-LYG (PMC7516720). - Underlying immunodeficiency status and ability to achieve immune reconstitution (e.g., HIV control, reduction of iatrogenic immunosuppression) favorably affect outcome.

Complications: Progression to overt EBV+ diffuse large B-cell lymphoma (grade 3 transformation), organ failure from pulmonary/hepatic/renal destruction, secondary infections related to immunosuppressive therapy, hemophagocytic lymphohistiocytosis as a rare severe complication.


12. Treatment

Modern risk/grade-stratified paradigm (NCI standard of care, established by the Lancet Haematology 2023 phase 2 trial, PMID 37011643):

  • Low-grade disease (grades 1–2) — immunotherapy-first, reflecting "immune-dependent" biology:
  • Interferon alfa-2b — dose-escalated subcutaneous injections, starting ~7.5 million international units three times weekly, continued for up to 1 year past best response. NCIT suggestion: NCIT:C1666 (Interferon Alfa-2b); treatment_term NCIT:C15986 (Pharmacotherapy).

  • High-grade disease (grade 3) — chemoimmunotherapy, reflecting "immune-independent," frankly malignant biology:

  • DA-EPOCH-R (dose-adjusted etoposide, prednisone, vincristine, cyclophosphamide, doxorubicin, and rituximab) — six cycles every 3 weeks intravenously. NCIT suggestion: regimen conceptually analogous to standard aggressive B-cell lymphoma chemoimmunotherapy; therapeutic_agent components include rituximab (CHEBI/NCIT:C2185 or similar anti-CD20 monoclonal antibody term), doxorubicin, cyclophosphamide, vincristine, etoposide, prednisone.

  • Rituximab monotherapy — reported in isolated case reports (e.g., mediastinal LYG achieving complete remission after 3 months of rituximab monotherapy, PMID 15693798), but with variable/unpredictable results as monotherapy; not established as standard for high-grade disease alone.

Immune reconstitution as therapy: In cases secondary to reversible immunosuppression (e.g., newly diagnosed HIV, TNF-α inhibitor use, post-transplant), reducing/discontinuing the offending immunosuppression or initiating antiretroviral therapy has produced remission of low-grade pulmonary LYG, underscoring the immune-dependent mechanism at low grade (PMC11829542).

Emerging/experimental therapies: - PD-1 checkpoint inhibition — a case report describes successful treatment of pulmonary LYG with a PD-1 inhibitor-based regimen, suggesting a role for immune checkpoint blockade as an emerging strategy, though this is not yet standard of care and mechanistic rationale (tumor PD-L1 engagement suppressing anti-EBV T-cell responses) is inferred by analogy to other EBV+ lymphomas rather than LYG-specific mechanistic data. - Hematopoietic stem cell transplantation — curative for LYG arising in the context of an underlying correctable primary immunodeficiency (e.g., DOCK8 deficiency), where HSCT resolved both the immunodeficiency and the LYG.

Supportive care: Management of pulmonary complications (may require ventilatory support in severe disease), CNS-directed therapy (high-dose methotrexate plus rituximab reported effective for higher-grade CNS-LYG), and standard supportive oncologic care.

Treatment algorithm summary: The defining modern conceptual advance is that LYG is not treated as a single entity but is bifurcated by grade into a proposed "immune-dependent" (low-grade, treat the immune deficit) versus "immune-independent" (high-grade, treat as lymphoma) disease model — directly informing the two-arm design of the pivotal 2023 trial.


13. Prevention

Given LYG's basis in EBV plus acquired/host immune dysfunction, prevention is indirect rather than primary (no LYG-specific vaccine or prophylactic agent exists):

  • Primary prevention: No EBV vaccine is currently licensed for general use (EBV vaccine candidates remain in clinical trials for EBV-associated diseases broadly, not LYG-specific); minimizing unnecessary or prolonged immunosuppression, and judicious use of TNF-α inhibitors and other immunomodulatory agents with awareness of EBV-driven lymphoproliferative risk, represents a practical primary-prevention-adjacent strategy in susceptible patients.
  • Secondary prevention/early detection: Surveillance for EBV-driven lymphoproliferative disease (including LYG) in patients with known primary immunodeficiency syndromes (DOCK8 deficiency, Wiskott-Aldrich syndrome, XLP, CVID), solid organ transplant recipients, and HIV-positive patients with poor immune control — enabling earlier diagnosis and grade-stratified treatment before high-grade transformation.
  • Tertiary prevention: Early initiation of interferon alfa-2b in low-grade disease is explicitly framed (per the 2023 NCI trial) as reducing the risk of progression to high-grade, immune-independent disease — i.e., tertiary prevention of transformation.
  • Genetic counseling: Relevant when LYG arises in the context of an underlying heritable immunodeficiency syndrome (e.g., DOCK8 deficiency, Wiskott-Aldrich syndrome), where standard genetic counseling for the primary condition applies, including consideration of carrier testing and reproductive counseling for at-risk relatives.
  • Public health/prophylaxis: No specific public health intervention targets LYG; general EBV exposure is nearly universal and not itself modifiable at the population level.

14. Other Species / Natural Disease

No naturally occurring veterinary counterpart of lymphomatoid granulomatosis specifically was identified in this search (unlike, e.g., some EBV-associated human lymphomas that have partial analogs in Old World primate lymphocryptovirus infections). EBV itself is a human-tropic gammaherpesvirus (NCBITaxon:10376) without natural non-human hosts, though related lymphocryptoviruses infect other primates and can produce analogous lymphoproliferative disease in those species (relevant to comparative biology of gammaherpesvirus-driven lymphoproliferation broadly, though not documented as "lymphomatoid granulomatosis" by name in veterinary literature). No OMIA (Online Mendelian Inheritance in Animals) entry or zoonotic transmission pathway is applicable, since EBV is not zoonotic.


15. Model Organisms

No LYG-specific animal or in vitro model was identified in the literature searched. However, the broader mechanistic paradigm — EBV infection with defective T-cell immunosurveillance driving B-cell lymphoproliferation — is modeled by:

  • Humanized mouse models of EBV infection (e.g., NOD/Shi-scid IL2rγnull [NOG], NOD/LtSz-scid Il2rg−/− [NSG], BALB/c Rag2−/−Il2rg−/− strains reconstituted with human hematopoietic stem cells) — these models reproduce EBV-associated B-cell lymphoproliferative disease and hemophagocytic lymphohistiocytosis, and are used to study post-transplant lymphoproliferative disease (PTLD), a mechanistically related EBV+ B-cell lymphoproliferation arising under iatrogenic immunosuppression (PMC4235711, "Humanized Mouse Models of Epstein-Barr Virus Infection and Associated Diseases").
  • Immunosuppression-augmented humanized mouse models — e.g., FK506 (tacrolimus) treatment of humanized mice increases the frequency of EBV-associated lymphoproliferative disease, directly modeling the iatrogenic-immunosuppression risk pathway relevant to LYG in transplant recipients (PMC7162544).
  • Checkpoint blockade studies in humanized mice — PD-1/CTLA-4 blockade has been shown to inhibit EBV-induced lymphoma growth in a cord-blood humanized mouse model, providing translational rationale (by analogy, not direct LYG data) for the emerging PD-1 inhibitor case report noted above (PMC4871349).

Model limitations relevant to LYG specifically: Existing humanized-mouse EBV-lymphoproliferation models capture generic EBV+ B-cell lymphoproliferative disease and PTLD-like biology but do not recapitulate the LYG-defining angiocentric/angioinvasive tissue tropism, multi-organ pattern (lung/skin/CNS predominance), or the specific CD4-predominant reactive T-cell microenvironment — representing a clear gap between available model systems and the human disease phenotype that would need explicit flagging (e.g., a HUMAN_MODEL_MISMATCH framing) in any KB curation.


Summary of Suggested Ontology Bindings for KB Curation

Concept Suggested term
Disease MONDO:0019466 (lymphomatoid granulomatosis)
Causal/associated agent EBV — NCBITaxon:10376
Neoplastic cell CL:0000236 (B cell), EBV-transformed large B cell
Reactive infiltrate cell CL:0000624 (CD4+ alpha-beta T cell)
Lung involvement UBERON:0002048
Skin involvement UBERON:0002097
CNS involvement UBERON:0000955
Angiodestruction/necrosis GO process terms for apoptosis/necrosis, tissue damage
Treatment — interferon NCIT:C1666 (Interferon Alfa-2b) under NCIT:C15986 (Pharmacotherapy)
Treatment — DA-EPOCH-R NCIT:C15632 (Chemotherapy) + therapeutic_agent list (rituximab, doxorubicin, cyclophosphamide, vincristine, etoposide, prednisone); consider regimen_term if an NCIT-coded DA-EPOCH-R identity exists
Genetic predisposition (when applicable) hgnc:2993 (DOCK8), WAS gene

Sources

Reference Validation

Checked with linkml-reference-validator 0.2.1.

Outcome Count
References checked 24
Resolved 24
Unresolved (possible confabulation) 0
Unverifiable 0
References weighed for topical relevance 24
On topic 12
Off topic 0

All extracted references resolved successfully.

Term Validation

Checked with linkml-term-validator 0.4.5, through the ols: adapter.

Outcome Count
Terms checked 29
Resolved 27
Unresolved (possible confabulation) 1
Obsolete 0
Unverifiable 1
Terms whose name was checked 18
Terms named correctly 11
Terms named as a different term 2
Terms whose name is worth a second look 5

Terms the report names something else

These identifiers resolve, so nothing about them looks wrong, and the ontology calls them something unrelated to what the report calls them. That usually means the identifier is not the one the sentence needs:

  • UBERON:0000955 (2 mentions) - the report calls it "CNS involvement"; UBERON calls it brain
  • NCIT:C1666 (2 mentions) - the report calls it "Interferon Alfa-2b"; NCIT calls it Tyrphostin A30

Unresolved terms

These identifiers do not exist in an ontology that resolved other terms from the same prefix, so they were most likely invented:

  • HP:0002316 (1 mention) - HP does not contain this term

Terms whose name is worth a second look

The report's name for these is recognisably related to the term's own name without being one of them. A loose paraphrase reads the same way as a citation of the wrong sibling term - and so does a related synonym, which the ontology records precisely because it names something adjacent rather than the same thing - so these are listed rather than judged:

  • HP:0001392 (1 mention) - the report calls it "Abnormal liver morphology"; HP calls it Abnormality of the liver, and lists "Abnormal liver" among its other names
  • GO:0032102 (1 mention) - the report calls it "negative regulation of response to wounding"; GO calls it negative regulation of response to external stimulus
  • CL:0000624 (3 mentions) - the report calls it "CD4-positive, alpha-beta T cell", "CD4+ alpha-beta T cell"; CL calls it CD4-positive, alpha-beta T cell
  • UBERON:0002048 (2 mentions) - the report calls it "lung", "Lung involvement"; UBERON calls it lung
  • UBERON:0002097 (2 mentions) - the report calls it "Skin involvement"; UBERON calls it skin of body, and lists "entire integument" among its other names

Terms named inconsistently

The report gives these identifiers more than one name of its own:

  • CL:0000624 - called "CD4-positive, alpha-beta T cell", "CD4+ alpha-beta T cell"
  • UBERON:0002048 - called "lung", "Lung involvement"

Prefixes with no resolver

Terms carrying these prefixes were not checked either way, because no configured ontology covers them. An unrecognised prefix may name an ontology this run could not reach as easily as one that does not exist, so nothing here is evidence of fabrication: ORPHA.