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6
Pathophys.
11
Phenotypes
18
Pathograph
1
Genes
6
Medical Actions
1
References
🏷

Classifications

Harrison's Chapter
GENETICS_ENVIRONMENT_DISEASE

Pathophysiology

6
CPT1A enzyme deficiency
Biallelic pathogenic variants in CPT1A reduce the activity of the liver isoform of carnitine palmitoyltransferase 1 on the outer mitochondrial membrane. CPT1A catalyzes conjugation of long-chain acyl-CoA to carnitine to form long-chain acylcarnitine, the first and rate-limiting step of the carnitine shuttle. Residual enzyme activity is typically 1%-5% in affected individuals.
hepatocyte CL:0000182
CPT1A hgnc:2328
carnitine shuttle GO:0006853 ↓ DECREASED
carnitine O-palmitoyltransferase activity GO:0004095 ↓ DECREASED
mitochondrial outer membrane GO:0005741
Show evidence (3 references)
PMID:21763168 SUPPORT Human Clinical
"Carnitine palmitoyltransferase 1A (CPT1A) catalyzes the synthesis of long chain acylcarnitines and is required for mitochondrial fatty acid oxidation in the liver."
Defines the CPT1A-catalyzed reaction (synthesis of long-chain acylcarnitines) as required for hepatic mitochondrial fatty acid oxidation.
PMID:20301700 SUPPORT Human Clinical
"Residual enzyme activity is 1%-5% in"
GeneReviews documents residual CPT1 enzyme activity of 1%-5% in most individuals with CPT1A deficiency.
PMID:22301540 SUPPORT Other
"palmitoyltransferase-1A is the rate-limiting enzyme that allows the body to"
Review identifies CPT1A as the rate-limiting enzyme for using fat for energy during fasting and illness.
Carnitine shuttle first step
The carnitine shuttle imports long-chain fatty acids into the mitochondrial matrix in three steps: CPT1 on the outer mitochondrial membrane converts long-chain acyl-CoA to acylcarnitine, CACT translocates acylcarnitine across the inner membrane, and CPT2 regenerates acyl-CoA in the matrix. CPT1A is the liver isoform of the outer-membrane CPT1 step, so its loss blocks the shuttle at its committed, rate-limiting entry point.
carnitine shuttle GO:0006853
mitochondrial outer membrane GO:0005741
Show evidence (1 reference)
PMID:15363638 SUPPORT Other
"proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes."
Establishes that the CPT system comprises outer-membrane CPT1 and inner-membrane CPT2 proteins.
Impaired hepatic long-chain fatty acid oxidation
Loss of CPT1A blocks hepatic oxidation of long-chain fatty acids. During fasting or illness, when hepatic glycogen is depleted and the liver normally switches to fatty acid oxidation to fuel ketogenesis and gluconeogenesis, this block leaves the liver unable to generate ketones or sustain energy production, and long-chain acyl groups cannot be esterified to carnitine for import.
hepatocyte CL:0000182
fatty acid beta-oxidation GO:0006635 ↓ DECREASED long-chain fatty acid metabolic process GO:0001676 ↓ DECREASED
Show evidence (1 reference)
PMID:20301700 SUPPORT Human Clinical
"is a disorder of long-chain fatty acid oxidation."
GeneReviews characterizes CPT1A deficiency as a disorder of long-chain fatty acid oxidation.
Distinctive carnitine and acylcarnitine signature
Unlike most fatty acid oxidation disorders, which accumulate acylcarnitines, CPT1A deficiency cannot form long-chain acylcarnitines. The result is low total and long-chain acylcarnitines with high free carnitine and an elevated free-carnitine to long-chain-acylcarnitine ratio [C0/(C16 + C18)], the diagnostic biochemical hallmark used on newborn screening.
Show evidence (2 references)
PMID:41017303 SUPPORT Human Clinical
"comprise elevated concentrations of free"
Human case series identifies elevated free carnitine and an elevated metabolite ratio as the diagnostic abnormalities of CPT1A deficiency.
PMID:28748224 SUPPORT Human Clinical
"an elevated free-to-total carnitine ratio,"
Human case report documents a low proportion of acylcarnitine species and an elevated free-to-total carnitine ratio in CPT1A deficiency.
Sparing of muscle and heart
Carnitine palmitoyltransferase 1 exists as tissue-specific isoforms: the liver isoform CPT1A, the muscle isoform CPT1B, and the brain isoform CPT1C. Because skeletal muscle and heart depend on CPT1B rather than CPT1A, CPT1A deficiency is largely restricted to a hepatic phenotype and characteristically spares cardiac and skeletal muscle, contrasting with CPT2, CACT, and VLCAD deficiencies, which cause cardiomyopathy and rhabdomyolysis.
carnitine O-palmitoyltransferase activity GO:0004095
Show evidence (2 references)
PMID:15363638 SUPPORT Other
"While CPT2 is an ubiquitous protein, three tissue-specific CPT1 isoforms--the"
Establishes the tissue-specific CPT1 isoforms (liver CPT1A, muscle CPT1B, brain CPT1C) that explain the muscle-sparing hepatic phenotype of CPT1A deficiency.
PMID:15363638 SUPPORT Other
"attacks of fasting hypoketotic hypoglycemia."
The isolated fasting hypoketotic-hypoglycemia presentation of CPT1-A deficiency, without myopathy, reflects loss of the liver isoform only.
Hypoketotic hypoglycemia
During fasting or intercurrent illness, hepatic glycogen is depleted and the liver normally relies on fatty acid oxidation to fuel ketogenesis. CPT1A deficiency blocks this response, producing hypoglycemia with inappropriately low ketones and acute neurologic decompensation.
ketone body biosynthetic process GO:0046951 ↓ DECREASED
Show evidence (1 reference)
PMID:22301540 SUPPORT Other
"various symptoms including hypoketotic hypoglycemia, lethargy, and seizures."
Review documents hypoketotic hypoglycemia, lethargy, and seizures as presenting features after illness.

Pathograph

Use the checkboxes to hide or show graph categories. Hover nodes for evidence and cross-linked metadata.
Referential integrity issues (2):
  • Target 'Hepatic crisis' (from 'Impaired hepatic long-chain fatty acid oxidation') not found in named elements
  • Target 'Sudden death' (from 'Hypoketotic hypoglycemia') not found in named elements
Pathograph: causal mechanism network for Carnitine Palmitoyltransferase 1A Deficiency Interactive directed graph showing how pathophysiology mechanisms, phenotypes, genetic factors and variants, experimental models, environmental triggers, and treatments relate through causal and linked edges.

Phenotypes

11
Digestive 2
Hepatomegaly FREQUENT Hepatomegaly HP:0002240
Show evidence (1 reference)
PMID:28748224 SUPPORT Human Clinical
"marked hepatomegaly on physical exam that was later confirmed with an abdominal"
Case report documents marked hepatomegaly in a child with CPT1A deficiency presenting during acute illness.
Hepatic failure Hepatic failure HP:0001399
Show evidence (1 reference)
PMID:20301700 SUPPORT Human Clinical
"hypoglycemia, and sudden onset of liver failure) and (2) later-onset"
GeneReviews lists sudden onset of liver failure among presentations of CPT1A deficiency.
Metabolism 4
Hypoketotic hypoglycemia FREQUENT Hypoketotic hypoglycemia HP:0001985
Show evidence (2 references)
PMID:15363638 SUPPORT Other
"attacks of fasting hypoketotic hypoglycemia."
Review identifies recurrent attacks of fasting hypoketotic hypoglycemia as the presentation of CPT1-A deficiency.
PMID:21763168 SUPPORT Human Clinical
"CPT1A deficiency is an inherited disorder of hepatic fatty acid oxidation that affects the tolerance to fasting and presents clinically with hypoketotic hypoglycemia."
Supervised-fast study confirms hypoketotic hypoglycemia as the clinical presentation of CPT1A deficiency.
Elevated hepatic transaminases Elevated circulating hepatic transaminase concentration HP:0002910
Show evidence (2 references)
PMID:28748224 SUPPORT Human Clinical
"elevated liver transaminases in the setting of acute gastroenteritis."
Case report documents disproportionately elevated liver transaminases with acute gastroenteritis in CPT1A deficiency.
PMID:20301700 SUPPORT Human Clinical
"serum concentrations of liver transaminases, ammonia, and creatine kinase."
GeneReviews lists elevated serum liver transaminases among later-onset manifestations.
Hyperammonemia Hyperammonemia HP:0001987
Show evidence (1 reference)
PMID:20301700 SUPPORT Human Clinical
"serum concentrations of liver transaminases, ammonia, and creatine kinase."
GeneReviews lists elevated serum ammonia among later-onset manifestations of CPT1A deficiency.
Elevated creatine kinase Elevated circulating creatine kinase concentration HP:0003236
Show evidence (1 reference)
PMID:20301700 SUPPORT Human Clinical
"serum concentrations of liver transaminases, ammonia, and creatine kinase."
GeneReviews lists elevated serum creatine kinase among later-onset manifestations of CPT1A deficiency.
Nervous System 3
Hepatic encephalopathy Hepatic encephalopathy HP:0002480
Show evidence (1 reference)
PMID:20301700 SUPPORT Human Clinical
"encephalopathy, hypoglycemia, absent or low levels of ketones, and elevated"
GeneReviews identifies hepatic encephalopathy with hypoglycemia and low ketones as a recognized presentation.
Lethargy Lethargy HP:0001254
Show evidence (1 reference)
PMID:22301540 SUPPORT Other
"various symptoms including hypoketotic hypoglycemia, lethargy, and seizures."
Review lists lethargy among symptoms after illness in CPT1A deficiency.
Seizure OCCASIONAL Seizure HP:0001250
Show evidence (1 reference)
PMID:22301540 SUPPORT Other
"various symptoms including hypoketotic hypoglycemia, lethargy, and seizures."
Review lists seizures among presenting symptoms in CPT1A deficiency.
Constitutional 1
Sudden infant death OCCASIONAL Sudden death HP:0001699
Show evidence (1 reference)
PMID:23231747 SUPPORT Human Clinical
"Columbia First Nations populations, was associated with unexpected infant death (SIDS/SUDI, infection) throughout"
Epidemiologic study associates CPT1A P479L homozygosity with unexpected infant death.
Other 1
Susceptibility to infectious illness
Show evidence (1 reference)
PMID:34295859 SUPPORT Human Clinical
"p.P479L homozygosity was associated with LRTI admission (aOR:2.88 95%CI:1.46-5.64)"
Cohort study links p.P479L homozygosity to increased lower respiratory tract infection admissions in Inuit children.
🧬

Genetic Associations

1
CPT1A gene variants
Gene: CPT1A hgnc:2328
Autosomal recessive
Show evidence (1 reference)
PMID:26010953 SUPPORT Human Clinical
"The latter has the highest frequency (0.955)"
Documents CPT1A p.P479L as the highest-frequency variant in the Nunavik Inuit population.
💊

Medical Actions

6
Avoidance of prolonged fasting and frequent feeding
Action: dietary intervention MAXO:0000088
Frequent feeding and avoidance of prolonged fasting are the cornerstone of management; infants should eat frequently and fasting should not exceed about 12 hours during illness, surgery, or procedures. Cornstarch may be used to extend overnight fasting tolerance.
Mechanism Target:
INHIBITS Hypoketotic hypoglycemia — Avoiding prolonged fasting removes the catabolic trigger for hypoketotic hypoglycemia.
Show evidence (1 reference)
PMID:20301700 SUPPORT Human Clinical
"individuals fasting should not last more than 12 hours during illness, surgery,"
GeneReviews advises limiting fasting to prevent hypoglycemia in CPT1A deficiency.
Show evidence (1 reference)
PMID:20301700 SUPPORT Human Clinical
"individuals fasting should not last more than 12 hours during illness, surgery,"
GeneReviews recommends limiting fasting duration to prevent hypoglycemia.
High-carbohydrate, low-long-chain-fat diet with MCT
Action: dietary intervention MAXO:0000088
A high-carbohydrate diet low in long-chain fat provides a constant supply of carbohydrate energy, supplemented with medium-chain triglycerides (MCT), which bypass the CPT1A-dependent step and can supply roughly one third of total calories.
Mechanism Target:
RESTORES Impaired hepatic long-chain fatty acid oxidation — MCT provides medium-chain fatty acids that enter mitochondria independent of the carnitine shuttle, bypassing the CPT1A block.
Show evidence (1 reference)
PMID:20301700 SUPPORT Human Clinical
"high-carbohydrate diet low in long-chain fat and frequent feeding supplemented"
GeneReviews recommends a high-carbohydrate diet low in long-chain fat with MCT/triheptanoin supplementation.
Show evidence (1 reference)
PMID:20301700 SUPPORT Human Clinical
"or medical procedures; adults need a high-carbohydrate, low-fat diet to provide"
GeneReviews recommends a high-carbohydrate, low-fat diet with MCT in CPT1A deficiency.
Triheptanoin or MCT oil
Action: dietary intervention MAXO:0000088
Targeted therapy provides medium-chain or odd-carbon (triheptanoin) fat that bypasses the CPT1A-dependent long-chain shuttle to supply mitochondrial substrate.
Show evidence (1 reference)
PMID:20301700 SUPPORT Human Clinical
"(an odd-carbon triglyceride) or MCT oil. Supportive care: Acute treatment"
GeneReviews lists triheptanoin (an odd-carbon triglyceride) or MCT oil as targeted therapies.
Emergency intravenous glucose
Action: Pharmacotherapy NCIT:C15986
Acute treatment of hypoglycemia uses intravenous fluid containing 10% dextrose, maintained until blood glucose normalizes and hepatic glycogen is repleted.
Mechanism Target:
RESTORES Hypoketotic hypoglycemia — Intravenous dextrose restores carbohydrate energy supply during acute decompensation.
Show evidence (1 reference)
PMID:20301700 SUPPORT Human Clinical
"includes prompt treatment of hypoglycemia with intravenous fluid containing 10%"
GeneReviews specifies prompt intravenous 10% dextrose for acute hypoglycemia.
Show evidence (1 reference)
PMID:20301700 SUPPORT Human Clinical
"includes prompt treatment of hypoglycemia with intravenous fluid containing 10%"
GeneReviews specifies intravenous 10% dextrose as acute treatment for hypoglycemia.
Avoidance of hepatotoxic agents
Action: supportive care MAXO:0000950
Potentially hepatotoxic agents such as valproate and salicylate should be avoided, as should prolonged fasting.
Show evidence (1 reference)
PMID:20301700 SUPPORT Human Clinical
"such as valproate and salicylate; viral or bacterial infections; avoid"
GeneReviews lists valproate and salicylate among agents to avoid in CPT1A deficiency.
Genetic counseling
Action: genetic counseling MAXO:0000079
CPT1A deficiency is autosomal recessive; genetic counseling and carrier/prenatal testing are offered to at-risk families.
Show evidence (1 reference)
PMID:20301700 SUPPORT Human Clinical
"CPT1A deficiency is inherited in an autosomal recessive"
GeneReviews describes autosomal recessive inheritance, the basis for genetic counseling.
🌍

Environmental Factors

2
Febrile or gastrointestinal illness
Concurrent febrile or gastrointestinal illness increases energy demand and precipitates acute metabolic decompensation in CPT1A deficiency.
Show evidence (1 reference)
PMID:28748224 SUPPORT Human Clinical
"elevated liver transaminases in the setting of acute gastroenteritis."
Case report shows acute gastroenteritis precipitating hepatic decompensation in CPT1A deficiency.
Fasting
Prolonged fasting depletes glycogen and forces reliance on fatty acid oxidation, unmasking the CPT1A block and triggering hypoketotic hypoglycemia.
Show evidence (1 reference)
PMID:21763168 SUPPORT Human Clinical
"CPT1A deficiency is an inherited disorder of hepatic fatty acid oxidation that affects the tolerance to fasting and presents clinically with hypoketotic hypoglycemia."
Supervised-fast study shows fasting intolerance and hypoketotic hypoglycemia in CPT1A deficiency.
🔬

Biochemical Markers

3
Free carnitine (C0) (INCREASED)
Context: Free carnitine is characteristically elevated in CPT1A deficiency because long-chain acyl groups cannot be esterified to carnitine, the reverse of the low-free-carnitine pattern seen in most fatty acid oxidation disorders.
Pathograph Readouts
Readout Of Distinctive carnitine and acylcarnitine signature Positive Diagnostic
Elevated free carnitine is a diagnostic readout of the CPT1A block on long-chain acylcarnitine formation.
Show evidence (1 reference)
PMID:41017303 SUPPORT Human Clinical
"comprise elevated concentrations of free"
Human case series identifies elevated free carnitine as a diagnostic abnormality of CPT1A deficiency.
Show evidence (1 reference)
PMID:41017303 SUPPORT Human Clinical
"comprise elevated concentrations of free"
Documents elevated free carnitine as a disease-specific biochemical marker of CPT1A deficiency.
Long-chain acylcarnitines (C16, C18) (DECREASED)
Context: Long-chain acylcarnitine species are low because CPT1A cannot form long-chain acylcarnitine from long-chain acyl-CoA, giving a low proportion of acylcarnitine species on the plasma profile.
Pathograph Readouts
Readout Of Distinctive carnitine and acylcarnitine signature Negative Diagnostic
Decreased long-chain acylcarnitines report the failure of the CPT1A-catalyzed acylcarnitine-forming step.
Show evidence (1 reference)
PMID:21763168 SUPPORT Human Clinical
"the ratio of free carnitine (C0) to the sum of the C16 and C18 acylcarnitines (C0/C16+C18) was significantly elevated (mean 199 ± 68, normal < 130 in infants), which is characteristic of CPT1A deficiency, reflecting the decreased ability to generate long chain acylcarnitines"
The elevated C0/(C16+C18) ratio reflects decreased generation of long-chain acylcarnitines.
Show evidence (1 reference)
PMID:28748224 SUPPORT Human Clinical
"an elevated free-to-total carnitine ratio,"
Case report notes a low proportion of acylcarnitine species from plasma with an elevated free-to-total carnitine ratio.
Free carnitine to long-chain acylcarnitine ratio [C0/(C16 + C18)] (INCREASED)
Context: The C0/(C16 + C18) ratio is the diagnostic newborn-screening marker for CPT1A deficiency and is markedly elevated because free carnitine is high while long-chain acylcarnitines are low.
Pathograph Readouts
Readout Of Distinctive carnitine and acylcarnitine signature Positive Diagnostic
The elevated C0/(C16+C18) ratio is the composite diagnostic readout of the CPT1A block.
Show evidence (1 reference)
PMID:41017303 SUPPORT Human Clinical
"Disease-specific and diagnostic abnormalities of CPT1A deficiency"
Identifies the elevated [C0/(C16 + C18)] blood ratio as a disease-specific diagnostic abnormality.
Show evidence (1 reference)
PMID:21763168 SUPPORT Human Clinical
"the ratio of free carnitine (C0) to the sum of the C16 and C18 acylcarnitines (C0/C16+C18) was significantly elevated (mean 199 ± 68, normal < 130 in infants), which is characteristic of CPT1A deficiency, reflecting the decreased ability to generate long chain acylcarnitines"
Directly documents the elevated C0/(C16+C18) ratio characteristic of CPT1A deficiency.
{ }

Source YAML

click to show
name: Carnitine Palmitoyltransferase 1A Deficiency
category: Mendelian
creation_date: '2026-07-02T00:00:00Z'
classifications:
  harrisons_chapter:
  - classification_value: GENETICS_ENVIRONMENT_DISEASE
synonyms:
- CPT1A deficiency
- CPT-1A deficiency
- CPT IA deficiency
- Carnitine palmitoyltransferase 1A deficiency
- Hepatic carnitine palmitoyltransferase deficiency
description: >
  Carnitine palmitoyltransferase 1A (CPT1A) deficiency is an autosomal recessive
  inborn error of long-chain fatty acid oxidation caused by biallelic pathogenic
  variants in CPT1A. CPT1A is the liver isoform of carnitine palmitoyltransferase 1,
  located on the outer mitochondrial membrane, where it catalyzes the first and
  rate-limiting step of the carnitine shuttle: conjugation of long-chain acyl-CoA
  to carnitine to form long-chain acylcarnitine for import into the mitochondrial
  matrix and subsequent beta-oxidation. Loss of CPT1A activity impairs hepatic
  long-chain fatty acid oxidation, so that during fasting or intercurrent febrile
  or gastrointestinal illness affected individuals develop hypoketotic
  hypoglycemia, hepatic encephalopathy, hepatomegaly, and elevated liver
  transaminases, ammonia, and creatine kinase. The biochemical signature is
  distinctive and essentially the mirror image of most fatty acid oxidation
  disorders: low total and long-chain acylcarnitines with high free carnitine and
  an elevated free-carnitine to long-chain-acylcarnitine ratio [C0/(C16 + C18)].
  Because skeletal muscle and heart rely on the separate CPT1B isoform, CPT1A
  deficiency characteristically spares cardiac and skeletal muscle, distinguishing
  it from CPT2, CACT, and VLCAD deficiencies. The p.P479L ("Arctic") variant is a
  common founder allele in Inuit, Alaska Native, and other northern populations and
  is associated with hypoketotic hypoglycemia, infectious illness, and elevated
  infant mortality/sudden unexpected infant death.
disease_term:
  preferred_term: carnitine palmitoyltransferase 1A deficiency
  term:
    id: MONDO:0009705
    label: carnitine palmitoyl transferase 1A deficiency
parents:
- Fatty Acid Oxidation Disorder
- Inborn Error of Metabolism
prevalence:
- notes: >-
    Classic CPT1A deficiency is ultra-rare. The p.P479L (c.1436C>T) founder allele,
    however, reaches extremely high frequency in Arctic Indigenous populations of
    Alaska, Canada, and Greenland; in a Nunavik Inuit exome study it was the
    highest-frequency variant at an allele frequency of 0.955, and in a Nunavut
    Inuit cohort the p.P479L allele frequency was 0.82.
  evidence:
  - reference: PMID:41017303
    reference_title: "Pitfalls in the diagnosis of carnitine palmitoyltransferase 1 deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "ultra-rare autosomal recessive disorder of the carnitine cycle caused by"
    explanation: Human case series describes CPT1A deficiency as an ultra-rare autosomal recessive disorder of the carnitine cycle.
  - reference: PMID:26010953
    reference_title: "Increased missense mutation burden of Fatty Acid metabolism related genes in nunavik inuit population."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "The latter has the highest frequency (0.955)"
    explanation: Nunavik Inuit exome study reports the CPT1A p.P479L Arctic founder variant at an allele frequency of 0.955.
  - reference: PMID:34295859
    reference_title: "Association of the CPT1A p.P479L Metabolic Gene Variant With Childhood Respiratory and Other Infectious Illness in Nunavut."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "The p.P479L allele frequency was 0.82."
    explanation: Nunavut Inuit cohort reports the p.P479L founder allele frequency at 0.82.
progression:
- notes: >-
    Individuals are typically asymptomatic between episodes and appear
    developmentally and cognitively normal unless prior metabolic decompensation
    has caused neurologic damage. Acute crises are precipitated by fasting or by
    concurrent febrile or gastrointestinal illness when energy demands rise, and
    onset of manifestations is usually rapid. With newborn screening and prevention
    of hypoglycemia, the risk of related neurologic injury is reduced.
pathophysiology:
- name: CPT1A enzyme deficiency
  description: >
    Biallelic pathogenic variants in CPT1A reduce the activity of the liver
    isoform of carnitine palmitoyltransferase 1 on the outer mitochondrial
    membrane. CPT1A catalyzes conjugation of long-chain acyl-CoA to carnitine to
    form long-chain acylcarnitine, the first and rate-limiting step of the
    carnitine shuttle. Residual enzyme activity is typically 1%-5% in affected
    individuals.
  genes:
  - preferred_term: CPT1A
    term:
      id: hgnc:2328
      label: CPT1A
  molecular_functions:
  - preferred_term: carnitine O-palmitoyltransferase activity
    term:
      id: GO:0004095
      label: carnitine O-palmitoyltransferase activity
    modifier: DECREASED
  biological_processes:
  - preferred_term: carnitine shuttle
    term:
      id: GO:0006853
      label: carnitine shuttle
    modifier: DECREASED
  locations:
  - preferred_term: mitochondrial outer membrane
    term:
      id: GO:0005741
      label: mitochondrial outer membrane
  cell_types:
  - preferred_term: hepatocyte
    term:
      id: CL:0000182
      label: hepatocyte
  evidence:
  - reference: PMID:21763168
    reference_title: "Impaired fasting tolerance among Alaska native children with a common carnitine palmitoyltransferase 1A sequence variant."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Carnitine palmitoyltransferase 1A (CPT1A) catalyzes the synthesis of long chain acylcarnitines and is required for mitochondrial fatty acid oxidation in the liver."
    explanation: Defines the CPT1A-catalyzed reaction (synthesis of long-chain acylcarnitines) as required for hepatic mitochondrial fatty acid oxidation.
  - reference: PMID:20301700
    reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Residual enzyme activity is 1%-5% in"
    explanation: GeneReviews documents residual CPT1 enzyme activity of 1%-5% in most individuals with CPT1A deficiency.
  - reference: PMID:22301540
    reference_title: "Carnitine palmitoyltransferase-1A deficiency: a look at classic and arctic variants."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "palmitoyltransferase-1A is the rate-limiting enzyme that allows the body to"
    explanation: Review identifies CPT1A as the rate-limiting enzyme for using fat for energy during fasting and illness.
  downstream:
  - target: Impaired hepatic long-chain fatty acid oxidation
    description: Loss of the rate-limiting CPT1A step blocks entry of long-chain fatty acids into hepatic mitochondrial beta-oxidation.
    causal_link_type: DIRECT
    evidence:
    - reference: PMID:21763168
      reference_title: "Impaired fasting tolerance among Alaska native children with a common carnitine palmitoyltransferase 1A sequence variant."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "CPT1A deficiency is an inherited disorder of hepatic fatty acid oxidation that affects the tolerance to fasting and presents clinically with hypoketotic hypoglycemia."
      explanation: Directly links CPT1A deficiency to impaired hepatic fatty acid oxidation, fasting intolerance, and hypoketotic hypoglycemia.
- name: Carnitine shuttle first step
  description: >
    The carnitine shuttle imports long-chain fatty acids into the mitochondrial
    matrix in three steps: CPT1 on the outer mitochondrial membrane converts
    long-chain acyl-CoA to acylcarnitine, CACT translocates acylcarnitine across
    the inner membrane, and CPT2 regenerates acyl-CoA in the matrix. CPT1A is the
    liver isoform of the outer-membrane CPT1 step, so its loss blocks the shuttle
    at its committed, rate-limiting entry point.
  biological_processes:
  - preferred_term: carnitine shuttle
    term:
      id: GO:0006853
      label: carnitine shuttle
  locations:
  - preferred_term: mitochondrial outer membrane
    term:
      id: GO:0005741
      label: mitochondrial outer membrane
  evidence:
  - reference: PMID:15363638
    reference_title: "Carnitine palmitoyltransferases 1 and 2: biochemical, molecular and medical aspects."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes."
    explanation: Establishes that the CPT system comprises outer-membrane CPT1 and inner-membrane CPT2 proteins.
  downstream:
  - target: Impaired hepatic long-chain fatty acid oxidation
    description: Blocking the outer-membrane CPT1A step prevents formation of long-chain acylcarnitine and downstream mitochondrial beta-oxidation.
    causal_link_type: DIRECT
    evidence:
    - reference: PMID:15363638
      reference_title: "Carnitine palmitoyltransferases 1 and 2: biochemical, molecular and medical aspects."
      supports: SUPPORT
      evidence_source: OTHER
      snippet: "CPT1-A deficiency presents as recurrent"
      explanation: Review states that CPT1-A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia, the clinical readout of the blocked shuttle.
- name: Impaired hepatic long-chain fatty acid oxidation
  description: >
    Loss of CPT1A blocks hepatic oxidation of long-chain fatty acids. During
    fasting or illness, when hepatic glycogen is depleted and the liver normally
    switches to fatty acid oxidation to fuel ketogenesis and gluconeogenesis, this
    block leaves the liver unable to generate ketones or sustain energy production,
    and long-chain acyl groups cannot be esterified to carnitine for import.
  biological_processes:
  - preferred_term: fatty acid beta-oxidation
    term:
      id: GO:0006635
      label: fatty acid beta-oxidation
    modifier: DECREASED
  - preferred_term: long-chain fatty acid metabolic process
    term:
      id: GO:0001676
      label: long-chain fatty acid metabolic process
    modifier: DECREASED
  chemical_entities:
  - preferred_term: ketone body
    term:
      id: CHEBI:73693
      label: ketone body
    modifier: DECREASED
  locations:
  - preferred_term: liver
    term:
      id: UBERON:0002107
      label: liver
  cell_types:
  - preferred_term: hepatocyte
    term:
      id: CL:0000182
      label: hepatocyte
  evidence:
  - reference: PMID:20301700
    reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "is a disorder of long-chain fatty acid oxidation."
    explanation: GeneReviews characterizes CPT1A deficiency as a disorder of long-chain fatty acid oxidation.
  downstream:
  - target: Hypoketotic hypoglycemia
    description: Impaired hepatic fatty acid oxidation prevents compensatory ketogenesis during fasting, producing hypoglycemia with inappropriately low ketones.
    causal_link_type: DIRECT
    evidence:
    - reference: PMID:21763168
      reference_title: "Impaired fasting tolerance among Alaska native children with a common carnitine palmitoyltransferase 1A sequence variant."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "at the termination of the fast the mean level of serum ketones was decreased"
      explanation: Supervised-fast study documents blunted ketone production in CPT1A-variant children, the biochemical basis of hypoketotic hypoglycemia.
  - target: Distinctive carnitine and acylcarnitine signature
    description: Inability to generate long-chain acylcarnitine lowers long-chain acylcarnitine species and raises free carnitine, giving an elevated C0/(C16 + C18) ratio.
    causal_link_type: DIRECT
    evidence:
    - reference: PMID:21763168
      reference_title: "Impaired fasting tolerance among Alaska native children with a common carnitine palmitoyltransferase 1A sequence variant."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "the ratio of free carnitine (C0) to the sum of the C16 and C18 acylcarnitines (C0/C16+C18) was significantly elevated (mean 199 ± 68, normal < 130 in infants), which is characteristic of CPT1A deficiency, reflecting the decreased ability to generate long chain acylcarnitines"
      explanation: Directly links the CPT1A block to the elevated C0/(C16+C18) ratio through decreased generation of long-chain acylcarnitines.
  - target: Hepatic crisis
    description: Acute hepatic energy failure during fasting or illness produces hepatomegaly, elevated transaminases, hyperammonemia, and hepatic encephalopathy.
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    intermediate_mechanisms:
    - Acute hepatic energy failure and impaired ureagenesis during metabolic decompensation.
    evidence:
    - reference: PMID:20301700
      reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "serum concentrations of liver transaminases, ammonia, and creatine kinase."
      explanation: GeneReviews lists elevated liver transaminases, ammonia, and creatine kinase among later-onset manifestations of hepatic decompensation.
- name: Distinctive carnitine and acylcarnitine signature
  description: >
    Unlike most fatty acid oxidation disorders, which accumulate acylcarnitines,
    CPT1A deficiency cannot form long-chain acylcarnitines. The result is low total
    and long-chain acylcarnitines with high free carnitine and an elevated
    free-carnitine to long-chain-acylcarnitine ratio [C0/(C16 + C18)], the
    diagnostic biochemical hallmark used on newborn screening.
  chemical_entities:
  - preferred_term: carnitine
    term:
      id: CHEBI:17126
      label: carnitine
    modifier: INCREASED
  evidence:
  - reference: PMID:41017303
    reference_title: "Pitfalls in the diagnosis of carnitine palmitoyltransferase 1 deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "comprise elevated concentrations of free"
    explanation: Human case series identifies elevated free carnitine and an elevated metabolite ratio as the diagnostic abnormalities of CPT1A deficiency.
  - reference: PMID:28748224
    reference_title: "Utility of Genetic Testing for Confirmation of Abnormal Newborn Screening in Disorders of Long-Chain Fatty Acids: A Missed Case of Carnitine Palmitoyltransferase 1A (CPT1A) Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "an elevated free-to-total carnitine ratio,"
    explanation: Human case report documents a low proportion of acylcarnitine species and an elevated free-to-total carnitine ratio in CPT1A deficiency.
- name: Sparing of muscle and heart
  description: >
    Carnitine palmitoyltransferase 1 exists as tissue-specific isoforms: the liver
    isoform CPT1A, the muscle isoform CPT1B, and the brain isoform CPT1C. Because
    skeletal muscle and heart depend on CPT1B rather than CPT1A, CPT1A deficiency
    is largely restricted to a hepatic phenotype and characteristically spares
    cardiac and skeletal muscle, contrasting with CPT2, CACT, and VLCAD
    deficiencies, which cause cardiomyopathy and rhabdomyolysis.
  molecular_functions:
  - preferred_term: carnitine O-palmitoyltransferase activity
    term:
      id: GO:0004095
      label: carnitine O-palmitoyltransferase activity
  evidence:
  - reference: PMID:15363638
    reference_title: "Carnitine palmitoyltransferases 1 and 2: biochemical, molecular and medical aspects."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "While CPT2 is an ubiquitous protein, three tissue-specific CPT1 isoforms--the"
    explanation: Establishes the tissue-specific CPT1 isoforms (liver CPT1A, muscle CPT1B, brain CPT1C) that explain the muscle-sparing hepatic phenotype of CPT1A deficiency.
  - reference: PMID:15363638
    reference_title: "Carnitine palmitoyltransferases 1 and 2: biochemical, molecular and medical aspects."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "attacks of fasting hypoketotic hypoglycemia."
    explanation: The isolated fasting hypoketotic-hypoglycemia presentation of CPT1-A deficiency, without myopathy, reflects loss of the liver isoform only.
- name: Hypoketotic hypoglycemia
  description: >
    During fasting or intercurrent illness, hepatic glycogen is depleted and the
    liver normally relies on fatty acid oxidation to fuel ketogenesis. CPT1A
    deficiency blocks this response, producing hypoglycemia with inappropriately
    low ketones and acute neurologic decompensation.
  biological_processes:
  - preferred_term: ketone body biosynthetic process
    term:
      id: GO:0046951
      label: ketone body biosynthetic process
    modifier: DECREASED
  chemical_entities:
  - preferred_term: glucose
    term:
      id: CHEBI:17234
      label: glucose
    modifier: DECREASED
  - preferred_term: ketone body
    term:
      id: CHEBI:73693
      label: ketone body
    modifier: DECREASED
  evidence:
  - reference: PMID:22301540
    reference_title: "Carnitine palmitoyltransferase-1A deficiency: a look at classic and arctic variants."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "various symptoms including hypoketotic hypoglycemia, lethargy, and seizures."
    explanation: Review documents hypoketotic hypoglycemia, lethargy, and seizures as presenting features after illness.
  downstream:
  - target: Hepatic encephalopathy
    description: Severe hypoketotic hypoglycemia and hepatic energy failure can produce hepatic encephalopathy.
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    intermediate_mechanisms:
    - Acute energy deprivation and hyperammonemia during hepatic decompensation.
    evidence:
    - reference: PMID:20301700
      reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "encephalopathy, hypoglycemia, absent or low levels of ketones, and elevated"
      explanation: GeneReviews lists hepatic encephalopathy with hypoglycemia and absent/low ketones among later-onset manifestations.
  - target: Lethargy
    description: Acute energy failure manifests as lethargy during metabolic decompensation.
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:22301540
      reference_title: "Carnitine palmitoyltransferase-1A deficiency: a look at classic and arctic variants."
      supports: SUPPORT
      evidence_source: OTHER
      snippet: "various symptoms including hypoketotic hypoglycemia, lethargy, and seizures."
      explanation: Review lists lethargy among symptoms after illness in CPT1A deficiency.
  - target: Seizure
    description: Severe hypoglycemia can progress to seizures.
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:22301540
      reference_title: "Carnitine palmitoyltransferase-1A deficiency: a look at classic and arctic variants."
      supports: SUPPORT
      evidence_source: OTHER
      snippet: "various symptoms including hypoketotic hypoglycemia, lethargy, and seizures."
      explanation: Review lists seizures among presenting symptoms in CPT1A deficiency.
  - target: Sudden death
    description: In Arctic founder-variant populations, p.P479L homozygosity is associated with unexpected/sudden infant death.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:23231747
      reference_title: "Causes and risk factors for infant mortality in Nunavut, Canada 1999-2011."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "Columbia First Nations populations, was associated with unexpected infant death (SIDS/SUDI, infection) throughout"
      explanation: Epidemiologic study associates CPT1A P479L homozygosity with unexpected infant death (SIDS/SUDI, infection).
phenotypes:
- name: Hypoketotic hypoglycemia
  frequency: FREQUENT
  description: >
    Fasting or illness-triggered hypoglycemia with inappropriately low or absent
    ketones, the hallmark acute presentation of CPT1A deficiency.
  phenotype_term:
    preferred_term: Hypoketotic hypoglycemia
    term:
      id: HP:0001985
      label: Hypoketotic hypoglycemia
  evidence:
  - reference: PMID:15363638
    reference_title: "Carnitine palmitoyltransferases 1 and 2: biochemical, molecular and medical aspects."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "attacks of fasting hypoketotic hypoglycemia."
    explanation: Review identifies recurrent attacks of fasting hypoketotic hypoglycemia as the presentation of CPT1-A deficiency.
  - reference: PMID:21763168
    reference_title: "Impaired fasting tolerance among Alaska native children with a common carnitine palmitoyltransferase 1A sequence variant."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "CPT1A deficiency is an inherited disorder of hepatic fatty acid oxidation that affects the tolerance to fasting and presents clinically with hypoketotic hypoglycemia."
    explanation: Supervised-fast study confirms hypoketotic hypoglycemia as the clinical presentation of CPT1A deficiency.
- name: Hepatomegaly
  frequency: FREQUENT
  description: >
    Enlarged, fat-laden liver during metabolic decompensation; may be a presenting
    finding with intercurrent illness.
  phenotype_term:
    preferred_term: Hepatomegaly
    term:
      id: HP:0002240
      label: Hepatomegaly
  evidence:
  - reference: PMID:28748224
    reference_title: "Utility of Genetic Testing for Confirmation of Abnormal Newborn Screening in Disorders of Long-Chain Fatty Acids: A Missed Case of Carnitine Palmitoyltransferase 1A (CPT1A) Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "marked hepatomegaly on physical exam that was later confirmed with an abdominal"
    explanation: Case report documents marked hepatomegaly in a child with CPT1A deficiency presenting during acute illness.
- name: Elevated hepatic transaminases
  description: >
    Disproportionately elevated liver transaminases during acute decompensation,
    reflecting hepatocellular stress.
  phenotype_term:
    preferred_term: Elevated hepatic transaminases
    term:
      id: HP:0002910
      label: Elevated circulating hepatic transaminase concentration
  evidence:
  - reference: PMID:28748224
    reference_title: "Utility of Genetic Testing for Confirmation of Abnormal Newborn Screening in Disorders of Long-Chain Fatty Acids: A Missed Case of Carnitine Palmitoyltransferase 1A (CPT1A) Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "elevated liver transaminases in the setting of acute gastroenteritis."
    explanation: Case report documents disproportionately elevated liver transaminases with acute gastroenteritis in CPT1A deficiency.
  - reference: PMID:20301700
    reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "serum concentrations of liver transaminases, ammonia, and creatine kinase."
    explanation: GeneReviews lists elevated serum liver transaminases among later-onset manifestations.
- name: Hyperammonemia
  description: >
    Elevated serum ammonia can occur during acute hepatic metabolic
    decompensation.
  phenotype_term:
    preferred_term: Hyperammonemia
    term:
      id: HP:0001987
      label: Hyperammonemia
  evidence:
  - reference: PMID:20301700
    reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "serum concentrations of liver transaminases, ammonia, and creatine kinase."
    explanation: GeneReviews lists elevated serum ammonia among later-onset manifestations of CPT1A deficiency.
- name: Elevated creatine kinase
  description: >
    Elevated serum creatine kinase can accompany acute metabolic decompensation in
    CPT1A deficiency.
  phenotype_term:
    preferred_term: Elevated circulating creatine kinase concentration
    term:
      id: HP:0003236
      label: Elevated circulating creatine kinase concentration
  evidence:
  - reference: PMID:20301700
    reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "serum concentrations of liver transaminases, ammonia, and creatine kinase."
    explanation: GeneReviews lists elevated serum creatine kinase among later-onset manifestations of CPT1A deficiency.
- name: Hepatic encephalopathy
  description: >
    Recurrent episodes of hepatic encephalopathy occur during metabolic
    decompensation; individuals are typically normal between episodes.
  phenotype_term:
    preferred_term: Hepatic encephalopathy
    term:
      id: HP:0002480
      label: Hepatic encephalopathy
  evidence:
  - reference: PMID:20301700
    reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "encephalopathy, hypoglycemia, absent or low levels of ketones, and elevated"
    explanation: GeneReviews identifies hepatic encephalopathy with hypoglycemia and low ketones as a recognized presentation.
- name: Hepatic failure
  description: >
    Sudden onset of liver failure can occur, particularly in individuals
    identified by an out-of-range newborn screen who then decompensate.
  phenotype_term:
    preferred_term: Hepatic failure
    term:
      id: HP:0001399
      label: Hepatic failure
  evidence:
  - reference: PMID:20301700
    reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "hypoglycemia, and sudden onset of liver failure) and (2) later-onset"
    explanation: GeneReviews lists sudden onset of liver failure among presentations of CPT1A deficiency.
- name: Lethargy
  description: >
    Progressive lethargy during metabolic decompensation following illness.
  phenotype_term:
    preferred_term: Lethargy
    term:
      id: HP:0001254
      label: Lethargy
  evidence:
  - reference: PMID:22301540
    reference_title: "Carnitine palmitoyltransferase-1A deficiency: a look at classic and arctic variants."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "various symptoms including hypoketotic hypoglycemia, lethargy, and seizures."
    explanation: Review lists lethargy among symptoms after illness in CPT1A deficiency.
- name: Seizure
  frequency: OCCASIONAL
  description: >
    Seizures may occur secondary to severe hypoglycemia during acute crises.
  phenotype_term:
    preferred_term: Seizure
    term:
      id: HP:0001250
      label: Seizure
  evidence:
  - reference: PMID:22301540
    reference_title: "Carnitine palmitoyltransferase-1A deficiency: a look at classic and arctic variants."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "various symptoms including hypoketotic hypoglycemia, lethargy, and seizures."
    explanation: Review lists seizures among presenting symptoms in CPT1A deficiency.
- name: Sudden infant death
  frequency: OCCASIONAL
  description: >
    In Arctic founder-variant populations, p.P479L homozygosity is associated with
    unexpected/sudden infant death (SIDS/SUDI).
  phenotype_term:
    preferred_term: Sudden death
    term:
      id: HP:0001699
      label: Sudden death
  context: Arctic p.P479L founder populations
  evidence:
  - reference: PMID:23231747
    reference_title: "Causes and risk factors for infant mortality in Nunavut, Canada 1999-2011."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Columbia First Nations populations, was associated with unexpected infant death (SIDS/SUDI, infection) throughout"
    explanation: Epidemiologic study associates CPT1A P479L homozygosity with unexpected infant death.
- name: Susceptibility to infectious illness
  description: >
    In Nunavut Inuit children, p.P479L homozygosity was associated with increased
    childhood infectious illness, including hospitalization for lower respiratory
    tract infection, otitis media, and gastroenteritis.
  context: Arctic p.P479L founder populations
  evidence:
  - reference: PMID:34295859
    reference_title: "Association of the CPT1A p.P479L Metabolic Gene Variant With Childhood Respiratory and Other Infectious Illness in Nunavut."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "p.P479L homozygosity was associated with LRTI admission (aOR:2.88 95%CI:1.46-5.64)"
    explanation: Cohort study links p.P479L homozygosity to increased lower respiratory tract infection admissions in Inuit children.
biochemical:
- name: Free carnitine (C0)
  presence: INCREASED
  context: >
    Free carnitine is characteristically elevated in CPT1A deficiency because
    long-chain acyl groups cannot be esterified to carnitine, the reverse of the
    low-free-carnitine pattern seen in most fatty acid oxidation disorders.
  biomarker_term:
    preferred_term: free carnitine
    term:
      id: CHEBI:17126
      label: carnitine
  readouts:
  - target: Distinctive carnitine and acylcarnitine signature
    relationship: READOUT_OF
    direction: POSITIVE
    endpoint_context: DIAGNOSTIC
    interpretation: Elevated free carnitine is a diagnostic readout of the CPT1A block on long-chain acylcarnitine formation.
    evidence:
    - reference: PMID:41017303
      reference_title: "Pitfalls in the diagnosis of carnitine palmitoyltransferase 1 deficiency."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "comprise elevated concentrations of free"
      explanation: Human case series identifies elevated free carnitine as a diagnostic abnormality of CPT1A deficiency.
  evidence:
  - reference: PMID:41017303
    reference_title: "Pitfalls in the diagnosis of carnitine palmitoyltransferase 1 deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "comprise elevated concentrations of free"
    explanation: Documents elevated free carnitine as a disease-specific biochemical marker of CPT1A deficiency.
- name: Long-chain acylcarnitines (C16, C18)
  presence: DECREASED
  context: >
    Long-chain acylcarnitine species are low because CPT1A cannot form long-chain
    acylcarnitine from long-chain acyl-CoA, giving a low proportion of
    acylcarnitine species on the plasma profile.
  readouts:
  - target: Distinctive carnitine and acylcarnitine signature
    relationship: READOUT_OF
    direction: NEGATIVE
    endpoint_context: DIAGNOSTIC
    interpretation: Decreased long-chain acylcarnitines report the failure of the CPT1A-catalyzed acylcarnitine-forming step.
    evidence:
    - reference: PMID:21763168
      reference_title: "Impaired fasting tolerance among Alaska native children with a common carnitine palmitoyltransferase 1A sequence variant."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "the ratio of free carnitine (C0) to the sum of the C16 and C18 acylcarnitines (C0/C16+C18) was significantly elevated (mean 199 ± 68, normal < 130 in infants), which is characteristic of CPT1A deficiency, reflecting the decreased ability to generate long chain acylcarnitines"
      explanation: The elevated C0/(C16+C18) ratio reflects decreased generation of long-chain acylcarnitines.
  evidence:
  - reference: PMID:28748224
    reference_title: "Utility of Genetic Testing for Confirmation of Abnormal Newborn Screening in Disorders of Long-Chain Fatty Acids: A Missed Case of Carnitine Palmitoyltransferase 1A (CPT1A) Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "an elevated free-to-total carnitine ratio,"
    explanation: Case report notes a low proportion of acylcarnitine species from plasma with an elevated free-to-total carnitine ratio.
- name: Free carnitine to long-chain acylcarnitine ratio [C0/(C16 + C18)]
  presence: INCREASED
  context: >
    The C0/(C16 + C18) ratio is the diagnostic newborn-screening marker for CPT1A
    deficiency and is markedly elevated because free carnitine is high while
    long-chain acylcarnitines are low.
  readouts:
  - target: Distinctive carnitine and acylcarnitine signature
    relationship: READOUT_OF
    direction: POSITIVE
    endpoint_context: DIAGNOSTIC
    interpretation: The elevated C0/(C16+C18) ratio is the composite diagnostic readout of the CPT1A block.
    evidence:
    - reference: PMID:41017303
      reference_title: "Pitfalls in the diagnosis of carnitine palmitoyltransferase 1 deficiency."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "Disease-specific and diagnostic abnormalities of CPT1A deficiency"
      explanation: Identifies the elevated [C0/(C16 + C18)] blood ratio as a disease-specific diagnostic abnormality.
  evidence:
  - reference: PMID:21763168
    reference_title: "Impaired fasting tolerance among Alaska native children with a common carnitine palmitoyltransferase 1A sequence variant."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "the ratio of free carnitine (C0) to the sum of the C16 and C18 acylcarnitines (C0/C16+C18) was significantly elevated (mean 199 ± 68, normal < 130 in infants), which is characteristic of CPT1A deficiency, reflecting the decreased ability to generate long chain acylcarnitines"
    explanation: Directly documents the elevated C0/(C16+C18) ratio characteristic of CPT1A deficiency.
genetic:
- name: CPT1A gene variants
  gene_term:
    preferred_term: CPT1A
    term:
      id: hgnc:2328
      label: CPT1A
  inheritance:
  - name: Autosomal recessive
    evidence:
    - reference: PMID:41017303
      reference_title: "Pitfalls in the diagnosis of carnitine palmitoyltransferase 1 deficiency."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "ultra-rare autosomal recessive disorder of the carnitine cycle caused by"
      explanation: States that CPT1A deficiency is an autosomal recessive disorder caused by biallelic CPT1A variants.
    - reference: PMID:20301700
      reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "CPT1A deficiency is inherited in an autosomal recessive"
      explanation: GeneReviews states CPT1A deficiency is inherited in an autosomal recessive manner.
  variants:
  - name: CPT1A - p.P479L (c.1436C>T, "Arctic" variant)
    description: >
      A proline-to-leucine substitution at residue 479, the common Arctic founder
      allele in Inuit, Alaska Native, and Greenland populations. It produces a
      hypomorphic enzyme with reduced activity and altered malonyl-CoA regulation
      and is associated with impaired fasting ketogenesis, hypoketotic
      hypoglycemia, infectious illness, and elevated infant mortality.
    evidence:
    - reference: PMID:21763168
      reference_title: "Impaired fasting tolerance among Alaska native children with a common carnitine palmitoyltransferase 1A sequence variant."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "This sequence variant results in a proline to leucine substitution at amino acid 479 (p.P479L) of the CPT1A protein."
      explanation: Defines the c.1436C>T variant as the p.P479L substitution in CPT1A.
    - reference: PMID:26010953
      reference_title: "Increased missense mutation burden of Fatty Acid metabolism related genes in nunavik inuit population."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "CPT1C, CPT2 and CRAT, including three novel variants and one Inuit specific"
      explanation: Population genetics study identifies CPT1A p.P479L as an Inuit-specific high-frequency variant.
  features: >
    CPT1A deficiency results from biallelic pathogenic variants in CPT1A encoding
    the liver isoform of carnitine palmitoyltransferase 1. The p.P479L Arctic
    founder allele is the predominant variant in northern Indigenous populations,
    where it reaches very high allele frequency, whereas classic severe disease
    outside these populations arises from private loss-of-function variants.
  evidence:
  - reference: PMID:26010953
    reference_title: "Increased missense mutation burden of Fatty Acid metabolism related genes in nunavik inuit population."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "The latter has the highest frequency (0.955)"
    explanation: Documents CPT1A p.P479L as the highest-frequency variant in the Nunavik Inuit population.
treatments:
- name: Avoidance of prolonged fasting and frequent feeding
  description: >
    Frequent feeding and avoidance of prolonged fasting are the cornerstone of
    management; infants should eat frequently and fasting should not exceed about
    12 hours during illness, surgery, or procedures. Cornstarch may be used to
    extend overnight fasting tolerance.
  treatment_term:
    preferred_term: dietary intervention
    term:
      id: MAXO:0000088
      label: dietary intervention
  target_mechanisms:
  - target: Hypoketotic hypoglycemia
    treatment_effect: INHIBITS
    description: Avoiding prolonged fasting removes the catabolic trigger for hypoketotic hypoglycemia.
    evidence:
    - reference: PMID:20301700
      reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "individuals fasting should not last more than 12 hours during illness, surgery,"
      explanation: GeneReviews advises limiting fasting to prevent hypoglycemia in CPT1A deficiency.
  evidence:
  - reference: PMID:20301700
    reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "individuals fasting should not last more than 12 hours during illness, surgery,"
    explanation: GeneReviews recommends limiting fasting duration to prevent hypoglycemia.
- name: High-carbohydrate, low-long-chain-fat diet with MCT
  description: >
    A high-carbohydrate diet low in long-chain fat provides a constant supply of
    carbohydrate energy, supplemented with medium-chain triglycerides (MCT), which
    bypass the CPT1A-dependent step and can supply roughly one third of total
    calories.
  treatment_term:
    preferred_term: dietary intervention
    term:
      id: MAXO:0000088
      label: dietary intervention
  target_mechanisms:
  - target: Impaired hepatic long-chain fatty acid oxidation
    treatment_effect: RESTORES
    description: MCT provides medium-chain fatty acids that enter mitochondria independent of the carnitine shuttle, bypassing the CPT1A block.
    evidence:
    - reference: PMID:20301700
      reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "high-carbohydrate diet low in long-chain fat and frequent feeding supplemented"
      explanation: GeneReviews recommends a high-carbohydrate diet low in long-chain fat with MCT/triheptanoin supplementation.
  evidence:
  - reference: PMID:20301700
    reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "or medical procedures; adults need a high-carbohydrate, low-fat diet to provide"
    explanation: GeneReviews recommends a high-carbohydrate, low-fat diet with MCT in CPT1A deficiency.
- name: Triheptanoin or MCT oil
  description: >
    Targeted therapy provides medium-chain or odd-carbon (triheptanoin) fat that
    bypasses the CPT1A-dependent long-chain shuttle to supply mitochondrial
    substrate.
  treatment_term:
    preferred_term: dietary intervention
    term:
      id: MAXO:0000088
      label: dietary intervention
  evidence:
  - reference: PMID:20301700
    reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "(an odd-carbon triglyceride) or MCT oil. Supportive care: Acute treatment"
    explanation: GeneReviews lists triheptanoin (an odd-carbon triglyceride) or MCT oil as targeted therapies.
- name: Emergency intravenous glucose
  description: >
    Acute treatment of hypoglycemia uses intravenous fluid containing 10% dextrose,
    maintained until blood glucose normalizes and hepatic glycogen is repleted.
  treatment_term:
    preferred_term: Pharmacotherapy
    term:
      id: NCIT:C15986
      label: Pharmacotherapy
  target_mechanisms:
  - target: Hypoketotic hypoglycemia
    treatment_effect: RESTORES
    description: Intravenous dextrose restores carbohydrate energy supply during acute decompensation.
    evidence:
    - reference: PMID:20301700
      reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: "includes prompt treatment of hypoglycemia with intravenous fluid containing 10%"
      explanation: GeneReviews specifies prompt intravenous 10% dextrose for acute hypoglycemia.
  evidence:
  - reference: PMID:20301700
    reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "includes prompt treatment of hypoglycemia with intravenous fluid containing 10%"
    explanation: GeneReviews specifies intravenous 10% dextrose as acute treatment for hypoglycemia.
- name: Avoidance of hepatotoxic agents
  description: >
    Potentially hepatotoxic agents such as valproate and salicylate should be
    avoided, as should prolonged fasting.
  treatment_term:
    preferred_term: supportive care
    term:
      id: MAXO:0000950
      label: supportive care
  evidence:
  - reference: PMID:20301700
    reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "such as valproate and salicylate; viral or bacterial infections; avoid"
    explanation: GeneReviews lists valproate and salicylate among agents to avoid in CPT1A deficiency.
- name: Genetic counseling
  description: >
    CPT1A deficiency is autosomal recessive; genetic counseling and carrier/prenatal
    testing are offered to at-risk families.
  treatment_term:
    preferred_term: genetic counseling
    term:
      id: MAXO:0000079
      label: genetic counseling
  evidence:
  - reference: PMID:20301700
    reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "CPT1A deficiency is inherited in an autosomal recessive"
    explanation: GeneReviews describes autosomal recessive inheritance, the basis for genetic counseling.
environmental:
- name: Febrile or gastrointestinal illness
  description: >
    Concurrent febrile or gastrointestinal illness increases energy demand and
    precipitates acute metabolic decompensation in CPT1A deficiency.
  evidence:
  - reference: PMID:28748224
    reference_title: "Utility of Genetic Testing for Confirmation of Abnormal Newborn Screening in Disorders of Long-Chain Fatty Acids: A Missed Case of Carnitine Palmitoyltransferase 1A (CPT1A) Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "elevated liver transaminases in the setting of acute gastroenteritis."
    explanation: Case report shows acute gastroenteritis precipitating hepatic decompensation in CPT1A deficiency.
- name: Fasting
  description: >
    Prolonged fasting depletes glycogen and forces reliance on fatty acid
    oxidation, unmasking the CPT1A block and triggering hypoketotic hypoglycemia.
  evidence:
  - reference: PMID:21763168
    reference_title: "Impaired fasting tolerance among Alaska native children with a common carnitine palmitoyltransferase 1A sequence variant."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "CPT1A deficiency is an inherited disorder of hepatic fatty acid oxidation that affects the tolerance to fasting and presents clinically with hypoketotic hypoglycemia."
    explanation: Supervised-fast study shows fasting intolerance and hypoketotic hypoglycemia in CPT1A deficiency.
notes: >
  CPT1A deficiency is mechanistically distinctive among fatty acid oxidation
  disorders. Because CPT1A is the outer-mitochondrial-membrane, rate-limiting,
  liver-specific step of the carnitine shuttle, deficiency produces a
  predominantly hepatic phenotype (fasting/illness-triggered hypoketotic
  hypoglycemia, hepatic encephalopathy, hepatomegaly, elevated transaminases and
  ammonia) while sparing skeletal and cardiac muscle, which use the separate CPT1B
  isoform. Its biochemical signature is the inverse of most FAODs: low long-chain
  acylcarnitines with high free carnitine and an elevated C0/(C16 + C18) ratio.
  Maternal acute fatty liver of pregnancy/HELLP is classically linked to fetal
  LCHAD/MTP deficiency; the GeneReviews entry notes a rare association of CPT1A
  fetal genotype with acute fatty liver of pregnancy, but the strong maternal
  association is with LCHAD/MTP and is not asserted here as a CPT1A mechanism.
  Renal tubular acidosis has been reported in CPT1A deficiency but was not
  documented in the sources cited here and is therefore omitted pending a
  verifiable reference.
diagnosis:
- name: Molecular and enzymatic diagnosis of CPT1A deficiency
  diagnosis_term:
    preferred_term: genetic testing
    term:
      id: MAXO:0000127
      label: genetic testing
  description: >-
    Diagnosis is established by biallelic pathogenic CPT1A variants on molecular
    genetic testing; diminished CPT1 enzyme activity in cultured skin fibroblasts
    is an available option in some countries when molecular testing is not
    definitive.
  results: Biallelic pathogenic CPT1A variants; residual CPT1 enzyme activity 1%-5%.
  evidence:
  - reference: PMID:20301700
    reference_title: "Carnitine Palmitoyltransferase 1A Deficiency."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The diagnosis of CPT1A deficiency is established in a proband with biallelic
      pathogenic variants in CPT1A identified by molecular genetic testing.
      Diminished carnitine palmitoyltransferase 1 (CPT1) enzyme activity on
      cultured skin fibroblasts when molecular genetic testing is not definitive
      may be an available option in some countries. Residual enzyme activity is
      1%-5% in most individuals with CPT1A deficiency.
    explanation: >-
      GeneReviews diagnosis/testing criteria for CPT1A deficiency (molecular
      testing, with fibroblast CPT1 enzyme activity as an adjunct).
references:
- reference: PMID:20301700
  title: "Carnitine Palmitoyltransferase 1A Deficiency."
  tags:
  - GeneReviews
  findings: []
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References & Deep Research

References

1
Carnitine Palmitoyltransferase 1A Deficiency.
No top-level findings curated for this source.