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1
Inheritance
8
Pathophys.
14
Phenotypes
28
Pathograph
1
Genes
2
Treatments
1
Trials
1
Deep Research
👪

Inheritance

1
Autosomal recessive inheritance HP:0000007
ALG12-CDG is an autosomal recessive condition caused by biallelic pathogenic ALG12 variants.
Autosomal recessive inheritance
Show evidence (2 references)
PMID:38717015 SUPPORT Human Clinical
"ALG12-CDG specifically is caused by biallelic pathogenic variants in ALG12."
The 2024 prenatal case report directly states biallelic ALG12 causation.
"ALG12 | HGNC:19358 | ALG12-congenital disorder of glycosylation | MONDO:0011783 | AR | Definitive"
ClinGen classifies the ALG12-ALG12-CDG relationship as definitive with autosomal recessive inheritance.

Pathophysiology

8
ALG12 mannosyltransferase deficiency
Pathogenic ALG12 variants impair the alpha-mannosyltransferase step needed for dolichol-linked oligosaccharide biosynthesis and N-glycan precursor formation.
ALG12 link
dolichol-linked oligosaccharide biosynthetic process link ↓ DECREASED
mannosyltransferase activity link ↓ DECREASED
endoplasmic reticulum link
Show evidence (3 references)
PMID:34467644 SUPPORT Human Clinical
"Congenital disorder of glycosylation type Ig (ALG12-CDG) is a rare inherited metabolic disease caused by a defect in alpha-mannosyltransferase 8, encoded by the ALG12 gene (22q13.33)."
This directly identifies the disease-causing ALG12 enzyme defect.
"The mechanism of pathogenicity is known to be loss-of-function."
ClinGen supports loss of ALG12 function as the curated mechanism.
PMID:12217961 SUPPORT Human Clinical
"In the patient's fibroblasts, the biosynthetic intermediate GlcNAc(2)Man(7) oligosaccharide was detected both on the lipid carrier dolichyl pyrophosphate and on newly synthesized glycoproteins, thus pointing to a defect in the dolichyl pyrophosphate-GlcNAc(2)Man(7)-dependent ALG12 alpha1,6..."
The original ALG12-CDG report directly localizes the ALG12 defect to dolichyl-pyrophosphate-linked oligosaccharide biosynthesis.
N-glycan precursor formation defect
Impaired N-glycan precursor formation leads to hypoglycosylation of transferrin and other plasma proteins, consistent with a type I CDG biochemical pattern.
protein N-linked glycosylation link ↓ DECREASED
Show evidence (2 references)
PMID:34467644 SUPPORT Human Clinical
"Due to a newborn Slovak patient's clinical and biochemical abnormalities, the isoelectric focusing of transferrin was performed with observed significant hypoglycosylation typical of CDG I."
The transferrin result directly supports a type I CDG hypoglycosylation pattern.
PMID:31529350 SUPPORT Human Clinical
"Intact serum transferrin showed, as expected for a CDG type I defect, underoccupancy of N-glycosylation sites."
This supports protein N-glycosylation underoccupancy as a downstream biochemical consequence.
Abnormal serum and IgG N-glycosylation
Abnormal N-glycosylation of serum and IgG proteins contributes to multisystem manifestations, including immune dysfunction and recurrent infections.
B cell link plasma cell link hepatocyte link
protein N-linked glycosylation link ⚠ ABNORMAL immunoglobulin mediated immune response link ↓ DECREASED
Show evidence (2 references)
PMID:31529350 SUPPORT Human Clinical
"ALG12-CDG is a severe multisystem disease associated with low to deficient serum immunoglobulins and recurrent infections."
The glycophenotype report links ALG12-CDG to low serum immunoglobulins and recurrent infections.
PMID:31529350 SUPPORT Human Clinical
"As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and it is associated with distinct, abnormal glycosylation of total serum and IgG N-glycans."
This supports abnormal IgG N-glycosylation as a disease-associated biochemical mechanism.
Neurodevelopmental and brain involvement
ALG12-CDG neurodevelopmental involvement includes developmental delay, intellectual disability, hypotonia, and reported brain anomalies.
nervous system development link ⚠ ABNORMAL
brain link
Show evidence (2 references)
PMID:38717015 SUPPORT Human Clinical
"Currently reported features of ALG12-CDG include: developmental delay, hypotonia, failure to thrive and/or short stature, brain anomalies, recurrent infections, hypogammaglobulinemia, coagulation abnormalities, and genitourinary abnormalities."
The prenatal phenotype report summarizes the neurologic and brain feature set reported in ALG12-CDG.
PMID:31481313 SUPPORT Human Clinical
"ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare disorder caused by a deficiency of dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase which presents with intellectual disability, hypotonia, dysmorphic features, low IgG levels with recurrent infections, male..."
The case-series abstract directly supports intellectual disability and hypotonia as neurologic manifestations.
Growth and feeding impairment
ALG12-CDG can impair somatic growth and feeding, presenting as failure to thrive, growth retardation, anorexia, or short stature.
Show evidence (2 references)
PMID:12217961 SUPPORT Human Clinical
"The severe disease was identified in a child presenting with psychomotor retardation, hypotonia, growth retardation, dysmorphic features and anorexia."
The original report describes growth retardation and anorexia in an ALG12-CDG child.
PMID:38717015 SUPPORT Human Clinical
"Currently reported features of ALG12-CDG include: developmental delay, hypotonia, failure to thrive and/or short stature, brain anomalies, recurrent infections, hypogammaglobulinemia, coagulation abnormalities, and genitourinary abnormalities."
The prenatal phenotype report summarizes failure to thrive and/or short stature in the broader ALG12-CDG phenotype.
Dysmorphic and genitourinary developmental involvement
ALG12-CDG can involve craniofacial dysmorphism and male genitourinary developmental abnormalities.
Show evidence (2 references)
PMID:31481313 SUPPORT Human Clinical
"ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare disorder caused by a deficiency of dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase which presents with intellectual disability, hypotonia, dysmorphic features, low IgG levels with recurrent infections, male..."
The case-series abstract lists dysmorphic features and male genital hypoplasia in ALG12-CDG.
"Common phenotypes include intellectual disability, hypotonia, dysmorphic features, coagulation abnormalities and genitourinary abnormalities."
ClinGen independently lists dysmorphic and genitourinary abnormalities as common ALG12-CDG phenotypes.
Renal and skeletal developmental involvement
Severe ALG12-CDG can involve prenatal renal malformation and skeletal-dysplasia-like abnormalities.
kidney development link ⚠ ABNORMAL skeletal system development link ⚠ ABNORMAL
kidney link skeletal system link
Show evidence (2 references)
PMID:38717015 SUPPORT Human Clinical
"In addition, skeletal abnormalities resembling a skeletal dysplasia including shortened long bones and talipes equinovarus have been seen in more severe neonatal presentation of this disorder."
The prenatal report supports skeletal-dysplasia-like abnormalities in severe neonatal ALG12-CDG.
PMID:38717015 SUPPORT Human Clinical
"We report on a case expanding the phenotype of ALG12-CDG to include bilateral, multicystic kidneys in a neonatal demise identified with homozygous pathogenic variants in the ALG12 gene at c.1001del (p.N334Tfs*15) through clinical trio exome sequencing."
The case report directly supports multicystic renal malformation in a severe prenatal ALG12-CDG presentation.
Adult cardioskeletal structural involvement
A mild adult ALG12-CDG presentation included ventricular septal defect and severe scoliosis; causal directness and possible modifier contributions are unresolved.
heart link skeletal system link
Show evidence (1 reference)
PMID:32530140 SUPPORT Human Clinical
"We here present an intriguing patient with an exceptional phenotype: 25-year-old women with a ventricular septal defect and severe idiopathic scoliosis but no facial dysmorphism, who dances as a professional, and has a University degree."
The adult case report supports the reported co-occurrence of VSD and severe scoliosis in a genetically diagnosed ALG12-CDG patient.

Pathograph

Use the checkboxes to hide or show graph categories. Hover nodes for evidence and cross-linked metadata.
Pathograph: causal mechanism network for ALG12-congenital disorder of glycosylation Interactive directed graph showing how pathophysiology mechanisms, phenotypes, genetic factors and variants, experimental models, environmental triggers, and treatments relate through causal and linked edges.

Phenotypes

14
Blood 2
Decreased circulating immunoglobulin concentration Decreased circulating immunoglobulin concentration (HP:0004313)
Show evidence (1 reference)
PMID:31529350 SUPPORT Human Clinical
"ALG12-CDG is a severe multisystem disease associated with low to deficient serum immunoglobulins and recurrent infections."
The glycophenotype report directly supports low serum immunoglobulins.
Abnormality of coagulation Abnormality of coagulation (HP:0001928)
Show evidence (2 references)
PMID:38717015 SUPPORT Human Clinical
"Currently reported features of ALG12-CDG include: developmental delay, hypotonia, failure to thrive and/or short stature, brain anomalies, recurrent infections, hypogammaglobulinemia, coagulation abnormalities, and genitourinary abnormalities."
The case-series abstract lists coagulation abnormalities.
PMID:32530140 SUPPORT Human Clinical
"Increased levels of hypoglycosylated forms of F XI (also with significant deficiency) and transferrin were also detected."
The adult case demonstrates hypoglycosylation and deficiency of a coagulation factor.
Cardiovascular 1
Ventricular septal defect Ventricular septal defect (HP:0001629)
Show evidence (1 reference)
PMID:32530140 SUPPORT Human Clinical
"We here present an intriguing patient with an exceptional phenotype: 25-year-old women with a ventricular septal defect and severe idiopathic scoliosis but no facial dysmorphism, who dances as a professional, and has a University degree."
The adult case report directly identifies ventricular septal defect in an ALG12-CDG patient.
Head and Neck 1
Facial dysmorphism Abnormal facial shape (HP:0001999)
Show evidence (2 references)
PMID:31481313 SUPPORT Human Clinical
"ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare disorder caused by a deficiency of dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase which presents with intellectual disability, hypotonia, dysmorphic features, low IgG levels with recurrent infections, male..."
The case-series abstract explicitly lists dysmorphic features in ALG12-CDG.
"Common phenotypes include intellectual disability, hypotonia, dysmorphic features, coagulation abnormalities and genitourinary abnormalities."
ClinGen independently lists dysmorphic features as common in ALG12-CDG.
Immune 1
Recurrent respiratory infections Recurrent respiratory infections (HP:0002205)
Show evidence (1 reference)
PMID:38717015 SUPPORT Human Clinical
"Currently reported features of ALG12-CDG include: developmental delay, hypotonia, failure to thrive and/or short stature, brain anomalies, recurrent infections, hypogammaglobulinemia, coagulation abnormalities, and genitourinary abnormalities."
The case-series abstract explicitly links low IgG levels with recurrent infections.
Musculoskeletal 3
Generalized hypotonia Generalized hypotonia (HP:0001290)
Show evidence (1 reference)
PMID:38717015 SUPPORT Human Clinical
"Currently reported features of ALG12-CDG include: developmental delay, hypotonia, failure to thrive and/or short stature, brain anomalies, recurrent infections, hypogammaglobulinemia, coagulation abnormalities, and genitourinary abnormalities."
The report lists hypotonia as a currently reported feature.
Skeletal dysplasia Skeletal dysplasia (HP:0002652)
Show evidence (1 reference)
PMID:38717015 SUPPORT Human Clinical
"In addition, skeletal abnormalities resembling a skeletal dysplasia including shortened long bones and talipes equinovarus have been seen in more severe neonatal presentation of this disorder."
The prenatal phenotype report directly supports a skeletal dysplasia-like phenotype in severe neonatal ALG12-CDG.
Scoliosis Scoliosis (HP:0002650)
Show evidence (1 reference)
PMID:32530140 SUPPORT Human Clinical
"We here present an intriguing patient with an exceptional phenotype: 25-year-old women with a ventricular septal defect and severe idiopathic scoliosis but no facial dysmorphism, who dances as a professional, and has a University degree."
The adult case report directly identifies severe scoliosis in an ALG12-CDG patient.
Nervous System 2
Global developmental delay Global developmental delay (HP:0001263)
Show evidence (1 reference)
PMID:38717015 SUPPORT Human Clinical
"Currently reported features of ALG12-CDG include: developmental delay, hypotonia, failure to thrive and/or short stature, brain anomalies, recurrent infections, hypogammaglobulinemia, coagulation abnormalities, and genitourinary abnormalities."
The prenatal phenotype report summarizes developmental delay among reported ALG12-CDG features.
Intellectual disability Intellectual disability (HP:0001249)
Show evidence (2 references)
PMID:31481313 SUPPORT Human Clinical
"ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare disorder caused by a deficiency of dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase which presents with intellectual disability, hypotonia, dysmorphic features, low IgG levels with recurrent infections, male..."
The case-series abstract directly lists intellectual disability among ALG12-CDG presenting features.
"Common phenotypes include intellectual disability, hypotonia, dysmorphic features, coagulation abnormalities and genitourinary abnormalities."
ClinGen names intellectual disability as a common phenotype for this gene-disease assertion.
Growth 2
Failure to thrive Failure to thrive (HP:0001508)
Show evidence (1 reference)
PMID:38717015 SUPPORT Human Clinical
"Currently reported features of ALG12-CDG include: developmental delay, hypotonia, failure to thrive and/or short stature, brain anomalies, recurrent infections, hypogammaglobulinemia, coagulation abnormalities, and genitourinary abnormalities."
The report lists failure to thrive among current features.
Short stature Short stature (HP:0004322)
Show evidence (1 reference)
PMID:38717015 SUPPORT Human Clinical
"Currently reported features of ALG12-CDG include: developmental delay, hypotonia, failure to thrive and/or short stature, brain anomalies, recurrent infections, hypogammaglobulinemia, coagulation abnormalities, and genitourinary abnormalities."
The report lists short stature as an alternate growth manifestation.
Other 2
Hypoplastic male external genitalia Hypoplastic male external genitalia (HP:0000050)
Show evidence (2 references)
PMID:31481313 SUPPORT Human Clinical
"ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare disorder caused by a deficiency of dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase which presents with intellectual disability, hypotonia, dysmorphic features, low IgG levels with recurrent infections, male..."
The Tahata case-series abstract directly lists male genital hypoplasia.
"Common phenotypes include intellectual disability, hypotonia, dysmorphic features, coagulation abnormalities and genitourinary abnormalities."
ClinGen supports genitourinary abnormalities as common in ALG12-CDG.
Multicystic kidney dysplasia Multicystic kidney dysplasia (HP:0000003)
Show evidence (1 reference)
PMID:38717015 SUPPORT Human Clinical
"We report on a case expanding the phenotype of ALG12-CDG to include bilateral, multicystic kidneys in a neonatal demise identified with homozygous pathogenic variants in the ALG12 gene at c.1001del (p.N334Tfs*15) through clinical trio exome sequencing."
This directly supports multicystic kidneys as a reported prenatal extension of the phenotype.
🧬

Genetic Associations

1
ALG12 (Biallelic loss-of-function variants)
Show evidence (3 references)
PMID:34467644 SUPPORT Human Clinical
"Genetic analysis of the coding regions of the ALG12 gene of the patient revealed a novel homozygous substitution mutation c.1439T>C p.(Leu480Pro) within Exon 10."
The case report documents a homozygous ALG12 variant in an affected patient.
PMID:38717015 SUPPORT Human Clinical
"We report on a case expanding the phenotype of ALG12-CDG to include bilateral, multicystic kidneys in a neonatal demise identified with homozygous pathogenic variants in the ALG12 gene at c.1001del (p.N334Tfs*15) through clinical trio exome sequencing."
The prenatal case report identifies a homozygous pathogenic ALG12 frameshift variant.
"In summary, there is definitive evidence to support the relationship between ALG12 and autosomal recessive ALG12 -congenital disorder of glycosylation."
ClinGen provides a deterministic structured assertion for the ALG12 gene-disease relationship.
💊

Treatments

2
Supportive multidisciplinary care
Action: Supportive Care NCIT:C15747
No ALG12-CDG-specific disease-modifying therapy is established in the fetched evidence; care is supportive and directed to infections, nutrition/growth, neurodevelopmental needs, coagulation abnormalities, and organ-specific complications.
Target Phenotypes: Recurrent respiratory infections Coagulation abnormalities
Show evidence (1 reference)
PMID:37644541 PARTIAL Other
"Since the first clinical report in 1980 of PMM2-CDG, the most common CDG worldwide, research made great strides, but nearly all of them are still missing a cure."
This CDG-wide review supports the lack of curative therapy for most CDGs; by itself it only partially supports ALG12-CDG-specific supportive care.
IVIG/SCIG immunoglobulin replacement therapy
Action: immunoglobulin infusion therapy MAXO:0001480
Agent: Therapeutic Immune Globulin
Intravenous or subcutaneous immunoglobulin replacement is a disease-specific supportive consideration for ALG12-CDG patients with low immunoglobulins and recurrent infections. Validator-cache evidence supports the antibody-deficiency indication, while direct published treatment-use details are limited.
Target Phenotypes: Hypogammaglobulinemia Recurrent respiratory infections
Show evidence (2 references)
PMID:31529350 PARTIAL Human Clinical
"ALG12-CDG is a severe multisystem disease associated with low to deficient serum immunoglobulins and recurrent infections."
This supports the hypogammaglobulinemia and recurrent-infection indication for immunoglobulin replacement; it does not by itself document treatment response.
PMID:31481313 PARTIAL Human Clinical
"ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare disorder caused by a deficiency of dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase which presents with intellectual disability, hypotonia, dysmorphic features, low IgG levels with recurrent infections, male..."
The case series supports the low-IgG and recurrent-infection phenotype targeted by immunoglobulin replacement, but the cached abstract does not directly quote treatment use.
🔬

Biochemical Markers

3
Type I transferrin hypoglycosylation pattern (Present)
Pathograph Readouts
Readout Of N-glycan precursor formation defect Present Absent Diagnostic
A type I transferrin hypoglycosylation pattern reports impaired N-glycosylation-site occupancy downstream of the ALG12 precursor defect.
Show evidence (1 reference)
PMID:34467644 SUPPORT Human Clinical
"Due to a newborn Slovak patient's clinical and biochemical abnormalities, the isoelectric focusing of transferrin was performed with observed significant hypoglycosylation typical of CDG I."
This directly supports transferrin isoelectric focusing as a biochemical marker of type I CDG in ALG12-CDG.
Aberrant serum oligomannose N-glycan profile (Present)
Pathograph Readouts
Readout Of ALG12 mannosyltransferase deficiency Present Absent Diagnostic
Accumulation of GlcNAc2Man5-7 with reduced GlcNAc2Man8-9 reports impaired ALG12 enzymatic activity.
Show evidence (1 reference)
PMID:34467644 SUPPORT Human Clinical
"Furthermore, analysis of neutral serum N-glycans by mass spectrometry revealed the accumulation of GlcNAc2Man5-7 and decreased levels of GlcNAc2Man8-9, which indicated impaired ALG12 enzymatic activity."
Serum N-glycan profiling directly supports an ALG12-associated oligomannose signature.
Hypoglycosylated antithrombin and factor XI (Present)
Pathograph Readouts
Readout Of Abnormal serum and IgG N-glycosylation Present Absent Diagnostic
Hypoglycosylated coagulation proteins report systemic plasma-protein hypoglycosylation caused by the ALG12-CDG glycosylation defect.
Show evidence (1 reference)
PMID:32530140 SUPPORT Human Clinical
"Congenital disorder of glycosylation diagnosis started through the identification of antithrombin deficiency without SERPINC1 defect and the detection of hypoglycosylated forms."
The adult case shows a coagulation-protein hypoglycosylation clue to diagnosis.
🔬

Clinical Trials

1
NCT04199000 RECRUITING
CDG-wide observational natural-history study relevant to genetically, enzymatically, or molecularly confirmed CDG subtypes such as ALG12-CDG.
Show evidence (1 reference)
clinicaltrials:NCT04199000 PARTIAL Human Clinical
"The purpose of this research is to study the natural history of congenital disorders of glycosylation and its causes and treatments."
The study is CDG-wide rather than ALG12-specific, so it partially supports relevance to ALG12-CDG natural-history research.
{ }

Source YAML

click to show
name: ALG12-congenital disorder of glycosylation
creation_date: "2026-05-10T18:41:07Z"
updated_date: "2026-05-18T05:43:18Z"
description: >-
  ALG12-congenital disorder of glycosylation is a rare autosomal recessive
  disorder of protein N-linked glycosylation caused by biallelic ALG12
  variants. Loss of ALG12 mannosyltransferase activity disrupts
  N-glycan-precursor formation, produces a type I congenital disorder of
  glycosylation biochemical pattern, and causes variable neurodevelopmental,
  immune, coagulation, growth, skeletal, cardiac, and prenatal manifestations.
category: Mendelian
disease_term:
  preferred_term: ALG12-congenital disorder of glycosylation
  term:
    id: MONDO:0011783
    label: ALG12-congenital disorder of glycosylation
parents:
- congenital disorder of glycosylation type I
- disorder of protein N-glycosylation
synonyms:
- ALG12-CDG
- CDG-Ig
- CDG1G
- congenital disorder of glycosylation type Ig
- mannosyltransferase 8 deficiency
inheritance:
- name: Autosomal recessive inheritance
  inheritance_term:
    preferred_term: Autosomal recessive inheritance
    term:
      id: HP:0000007
      label: Autosomal recessive inheritance
  description: >-
    ALG12-CDG is an autosomal recessive condition caused by biallelic
    pathogenic ALG12 variants.
  evidence:
  - reference: PMID:38717015
    reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      ALG12-CDG specifically is caused by biallelic pathogenic variants in
      ALG12.
    explanation: >-
      The 2024 prenatal case report directly states biallelic ALG12 causation.
  - reference: CGGV:assertion_21631e85-8133-4703-a77c-c046effb6e56-2023-02-15T200000.000Z
    reference_title: "ALG12 / ALG12-congenital disorder of glycosylation (Definitive)"
    supports: SUPPORT
    evidence_source: OTHER
    snippet: "ALG12 | HGNC:19358 | ALG12-congenital disorder of glycosylation | MONDO:0011783 | AR | Definitive"
    explanation: >-
      ClinGen classifies the ALG12-ALG12-CDG relationship as definitive with
      autosomal recessive inheritance.
prevalence:
- population: Global published literature
  notes: >-
    ALG12-CDG is extremely rare, with only small case reports and case series
    in the published literature.
  evidence:
  - reference: PMID:34467644
    reference_title: A novel homozygous mutation in the human ALG12 gene results in an aberrant profile of oligomannose N-glycans in patient's serum.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      To date, only 15 patients have been diagnosed with ALG12-CDG globally.
    explanation: >-
      The 2021 case report provides a direct published patient-count estimate.
progression:
- phase: Variable severity
  notes: >-
    ALG12-CDG severity ranges from mild adult presentations to fatal
    multisystem infantile disease.
  evidence:
  - reference: PMID:31481313
    reference_title: "Complex phenotypes in ALG12-congenital disorder of glycosylation (ALG12-CDG): Case series and review of the literature."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Our cases broaden the recognized genetic and phenotypic spectrum of this
      disorder and suggest a role for other genetic and environmental factors
      in modulating disease phenotype.
    explanation: >-
      The within-family case series directly supports broad clinical
      variability, including mild surviving adults and severe infantile death.
pathophysiology:
- name: ALG12 mannosyltransferase deficiency
  description: >-
    Pathogenic ALG12 variants impair the alpha-mannosyltransferase step needed
    for dolichol-linked oligosaccharide biosynthesis and N-glycan precursor
    formation.
  genes:
  - preferred_term: ALG12
    term:
      id: hgnc:19358
      label: ALG12
  biological_processes:
  - preferred_term: dolichol-linked oligosaccharide biosynthetic process
    modifier: DECREASED
    term:
      id: GO:0006488
      label: dolichol-linked oligosaccharide biosynthetic process
  molecular_functions:
  - preferred_term: mannosyltransferase activity
    modifier: DECREASED
    term:
      id: GO:0000030
      label: mannosyltransferase activity
  locations:
  - preferred_term: endoplasmic reticulum
    term:
      id: GO:0005783
      label: endoplasmic reticulum
  evidence:
  - reference: PMID:34467644
    reference_title: A novel homozygous mutation in the human ALG12 gene results in an aberrant profile of oligomannose N-glycans in patient's serum.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Congenital disorder of glycosylation type Ig (ALG12-CDG) is a rare
      inherited metabolic disease caused by a defect in alpha-mannosyltransferase
      8, encoded by the ALG12 gene (22q13.33).
    explanation: >-
      This directly identifies the disease-causing ALG12 enzyme defect.
  - reference: CGGV:assertion_21631e85-8133-4703-a77c-c046effb6e56-2023-02-15T200000.000Z
    reference_title: "ALG12 / ALG12-congenital disorder of glycosylation (Definitive)"
    supports: SUPPORT
    evidence_source: OTHER
    snippet: >-
      The mechanism of pathogenicity is known to be loss-of-function.
    explanation: >-
      ClinGen supports loss of ALG12 function as the curated mechanism.
  - reference: PMID:12217961
    reference_title: ALG12 mannosyltransferase defect in congenital disorder of glycosylation type lg.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      In the patient's fibroblasts, the biosynthetic intermediate
      GlcNAc(2)Man(7) oligosaccharide was detected both on the lipid carrier
      dolichyl pyrophosphate and on newly synthesized glycoproteins, thus
      pointing to a defect in the dolichyl pyrophosphate-GlcNAc(2)Man(7)-dependent
      ALG12 alpha1,6 mannosyltransferase.
    explanation: >-
      The original ALG12-CDG report directly localizes the ALG12 defect to
      dolichyl-pyrophosphate-linked oligosaccharide biosynthesis.
  downstream:
  - target: N-glycan precursor formation defect
    description: >-
      Loss of ALG12 function impairs N-glycan precursor formation and transfer
      to proteins.
    causal_link_type: DIRECT
    evidence:
    - reference: PMID:32530140
      reference_title: "ALG12-CDG: An unusual patient without intellectual disability and facial dysmorphism, and with a novel variant."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Congenital disorder of glycosylation (CDG) type I is a group of rare
        disorders caused by recessive mutations in up to 25 genes that impair
        the N-glycan precursor formation and its transfer to proteins resulting
        in hypoglycosylation of multiple proteins.
      explanation: >-
        The case report describes the biochemical class to which ALG12-CDG
        belongs and links recessive pathway-gene defects to impaired
        precursor formation.
- name: N-glycan precursor formation defect
  description: >-
    Impaired N-glycan precursor formation leads to hypoglycosylation of
    transferrin and other plasma proteins, consistent with a type I CDG
    biochemical pattern.
  biological_processes:
  - preferred_term: protein N-linked glycosylation
    modifier: DECREASED
    term:
      id: GO:0006487
      label: protein N-linked glycosylation
  chemical_entities:
  - preferred_term: N-glycan precursor
    modifier: ABNORMAL
    term:
      id: CHEBI:59520
      label: N-glycan
  evidence:
  - reference: PMID:34467644
    reference_title: A novel homozygous mutation in the human ALG12 gene results in an aberrant profile of oligomannose N-glycans in patient's serum.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Due to a newborn Slovak patient's clinical and biochemical abnormalities,
      the isoelectric focusing of transferrin was performed with observed
      significant hypoglycosylation typical of CDG I.
    explanation: >-
      The transferrin result directly supports a type I CDG hypoglycosylation
      pattern.
  - reference: PMID:31529350
    reference_title: "ALG12-CDG: novel glycophenotype insights endorse the molecular defect."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Intact serum transferrin showed, as expected for a CDG type I defect,
      underoccupancy of N-glycosylation sites.
    explanation: >-
      This supports protein N-glycosylation underoccupancy as a downstream
      biochemical consequence.
  downstream:
  - target: Abnormal serum and IgG N-glycosylation
    description: >-
      Impaired ALG12 activity shifts serum N-glycan structures toward
      truncated and abnormal high-mannose or hybrid species.
    causal_link_type: DIRECT
    evidence:
    - reference: PMID:34467644
      reference_title: A novel homozygous mutation in the human ALG12 gene results in an aberrant profile of oligomannose N-glycans in patient's serum.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Furthermore, analysis of neutral serum N-glycans by mass spectrometry
        revealed the accumulation of GlcNAc2Man5-7 and decreased levels of
        GlcNAc2Man8-9, which indicated impaired ALG12 enzymatic activity.
      explanation: >-
        Serum N-glycan mass spectrometry directly links abnormal N-glycan
        species to impaired ALG12 activity.
    - reference: PMID:31529350
      reference_title: "ALG12-CDG: novel glycophenotype insights endorse the molecular defect."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and
        it is associated with distinct, abnormal glycosylation of total serum
        and IgG N-glycans.
      explanation: >-
        This supports downstream abnormal glycosylation of total serum and IgG
        N-glycans.
  - target: Neurodevelopmental and brain involvement
    description: >-
      Abnormal N-glycan precursor formation affects developmentally important
      glycoproteins and is associated with neurologic and brain manifestations.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:32530140
      reference_title: "ALG12-CDG: An unusual patient without intellectual disability and facial dysmorphism, and with a novel variant."
      supports: PARTIAL
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        The relevance of N‐glycosylation for the folding, intracellular
        trafficking, and function of many proteins, particularly during
        development, support multiple mostly severe clinical phenotypes usually
        comprising also neurological involvement.
      explanation: >-
        The full-text clinical report explains why impaired N-glycosylation can
        produce developmental and neurologic disease, while the intermediates
        between ALG12 deficiency and individual neurologic signs remain
        unresolved.
  - target: Growth and feeding impairment
    description: >-
      The ALG12-linked glycosylation defect is associated with impaired growth
      and feeding failure in reported patients.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:12217961
      reference_title: ALG12 mannosyltransferase defect in congenital disorder of glycosylation type lg.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        The severe disease was identified in a child presenting with psychomotor
        retardation, hypotonia, growth retardation, dysmorphic features and
        anorexia.
      explanation: >-
        The original ALG12-CDG patient report links the ALG12 defect to growth
        retardation and anorexia.
  - target: Dysmorphic and genitourinary developmental involvement
    description: >-
      Multisystem ALG12-CDG presentations include craniofacial dysmorphism and
      male genital hypoplasia.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:31481313
      reference_title: "Complex phenotypes in ALG12-congenital disorder of glycosylation (ALG12-CDG): Case series and review of the literature."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare
        disorder caused by a deficiency of
        dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase
        which presents with intellectual disability, hypotonia, dysmorphic
        features, low IgG levels with recurrent infections, male genital
        hypoplasia, and coagulation abnormalities.
      explanation: >-
        The case-series abstract connects ALG12 mannosyltransferase deficiency
        to dysmorphic and genital developmental features.
  - target: Renal and skeletal developmental involvement
    description: >-
      Severe prenatal or neonatal ALG12-CDG presentations can include renal and
      skeletal developmental abnormalities.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:38717015
      reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        In addition, skeletal abnormalities resembling a skeletal dysplasia
        including shortened long bones and talipes equinovarus have been seen in
        more severe neonatal presentation of this disorder.
      explanation: >-
        The prenatal case report directly supports a severe neonatal skeletal
        branch of ALG12-CDG.
  - target: Adult cardioskeletal structural involvement
    description: >-
      An unusually mild adult presentation included congenital cardiac and
      spinal structural findings, with mechanism and modifier contribution still
      uncertain.
    causal_link_type: UNKNOWN
    evidence:
    - reference: PMID:32530140
      reference_title: "ALG12-CDG: An unusual patient without intellectual disability and facial dysmorphism, and with a novel variant."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        We here present an intriguing patient with an exceptional phenotype:
        25-year-old women with a ventricular septal defect and severe idiopathic
        scoliosis but no facial dysmorphism, who dances as a professional, and
        has a University degree.
      explanation: >-
        This supports a reported cardioskeletal structural branch, while the
        edge is marked unknown because the article discusses phenotype
        heterogeneity and possible modifiers.
- name: Abnormal serum and IgG N-glycosylation
  description: >-
    Abnormal N-glycosylation of serum and IgG proteins contributes to
    multisystem manifestations, including immune dysfunction and recurrent
    infections.
  cell_types:
  - preferred_term: B cell
    term:
      id: CL:0000236
      label: B cell
  - preferred_term: plasma cell
    term:
      id: CL:0000786
      label: plasma cell
  - preferred_term: hepatocyte
    term:
      id: CL:0000182
      label: hepatocyte
  biological_processes:
  - preferred_term: protein N-linked glycosylation
    modifier: ABNORMAL
    term:
      id: GO:0006487
      label: protein N-linked glycosylation
  - preferred_term: immunoglobulin mediated immune response
    modifier: DECREASED
    term:
      id: GO:0016064
      label: immunoglobulin mediated immune response
  evidence:
  - reference: PMID:31529350
    reference_title: "ALG12-CDG: novel glycophenotype insights endorse the molecular defect."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      ALG12-CDG is a severe multisystem disease associated with low to
      deficient serum immunoglobulins and recurrent infections.
    explanation: >-
      The glycophenotype report links ALG12-CDG to low serum immunoglobulins
      and recurrent infections.
  - reference: PMID:31529350
    reference_title: "ALG12-CDG: novel glycophenotype insights endorse the molecular defect."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      As a whole, ALG12-CDG behaves as a dual CDG (CDG-I and II defects) and it
      is associated with distinct, abnormal glycosylation of total serum and IgG
      N-glycans.
    explanation: >-
      This supports abnormal IgG N-glycosylation as a disease-associated
      biochemical mechanism.
  downstream:
  - target: Decreased circulating immunoglobulin concentration
    description: Abnormal IgG N-glycosylation and immune-system involvement contribute to hypogammaglobulinemia.
    evidence:
    - reference: PMID:31529350
      reference_title: "ALG12-CDG: novel glycophenotype insights endorse the molecular defect."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        ALG12-CDG is a severe multisystem disease associated with low to
        deficient serum immunoglobulins and recurrent infections.
      explanation: >-
        This directly supports the low-immunoglobulin downstream phenotype.
  - target: Recurrent respiratory infections
    description: Immunoglobulin deficiency contributes to recurrent infections.
    evidence:
    - reference: PMID:31529350
      reference_title: "ALG12-CDG: novel glycophenotype insights endorse the molecular defect."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        ALG12-CDG is a severe multisystem disease associated with low to
        deficient serum immunoglobulins and recurrent infections.
      explanation: >-
        This directly supports recurrent infections as a downstream
        immune-related manifestation.
  - target: Abnormality of coagulation
    description: Hypoglycosylation affects plasma proteins involved in coagulation.
    evidence:
    - reference: PMID:32530140
      reference_title: "ALG12-CDG: An unusual patient without intellectual disability and facial dysmorphism, and with a novel variant."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Increased levels of hypoglycosylated forms of F XI (also with
        significant deficiency) and transferrin were also detected.
      explanation: >-
        The adult case supports hypoglycosylated coagulation factors as a
        downstream manifestation of the glycosylation defect.
- name: Neurodevelopmental and brain involvement
  description: >-
    ALG12-CDG neurodevelopmental involvement includes developmental delay,
    intellectual disability, hypotonia, and reported brain anomalies.
  locations:
  - preferred_term: brain
    term:
      id: UBERON:0000955
      label: brain
  biological_processes:
  - preferred_term: nervous system development
    modifier: ABNORMAL
    term:
      id: GO:0007399
      label: nervous system development
  evidence:
  - reference: PMID:38717015
    reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Currently reported features of ALG12-CDG include: developmental delay,
      hypotonia, failure to thrive and/or short stature, brain anomalies,
      recurrent infections, hypogammaglobulinemia, coagulation abnormalities,
      and genitourinary abnormalities.
    explanation: >-
      The prenatal phenotype report summarizes the neurologic and brain feature
      set reported in ALG12-CDG.
  - reference: PMID:31481313
    reference_title: "Complex phenotypes in ALG12-congenital disorder of glycosylation (ALG12-CDG): Case series and review of the literature."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare disorder
      caused by a deficiency of
      dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase which
      presents with intellectual disability, hypotonia, dysmorphic features, low
      IgG levels with recurrent infections, male genital hypoplasia, and
      coagulation abnormalities.
    explanation: >-
      The case-series abstract directly supports intellectual disability and
      hypotonia as neurologic manifestations.
  downstream:
  - target: Global developmental delay
    description: Developmental delay is part of the reported ALG12-CDG neurologic spectrum.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:38717015
      reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Currently reported features of ALG12-CDG include: developmental delay,
        hypotonia, failure to thrive and/or short stature, brain anomalies,
        recurrent infections, hypogammaglobulinemia, coagulation abnormalities,
        and genitourinary abnormalities.
      explanation: The report lists developmental delay among ALG12-CDG features.
  - target: Intellectual disability
    description: Intellectual disability is part of the reported ALG12-CDG neurologic spectrum.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:31481313
      reference_title: "Complex phenotypes in ALG12-congenital disorder of glycosylation (ALG12-CDG): Case series and review of the literature."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare
        disorder caused by a deficiency of
        dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase
        which presents with intellectual disability, hypotonia, dysmorphic
        features, low IgG levels with recurrent infections, male genital
        hypoplasia, and coagulation abnormalities.
      explanation: The case-series abstract directly lists intellectual disability.
  - target: Generalized hypotonia
    description: Hypotonia is part of the reported ALG12-CDG neurologic spectrum.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:38717015
      reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Currently reported features of ALG12-CDG include: developmental delay,
        hypotonia, failure to thrive and/or short stature, brain anomalies,
        recurrent infections, hypogammaglobulinemia, coagulation abnormalities,
        and genitourinary abnormalities.
      explanation: The report lists hypotonia among ALG12-CDG features.
- name: Growth and feeding impairment
  description: >-
    ALG12-CDG can impair somatic growth and feeding, presenting as failure to
    thrive, growth retardation, anorexia, or short stature.
  evidence:
  - reference: PMID:12217961
    reference_title: ALG12 mannosyltransferase defect in congenital disorder of glycosylation type lg.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The severe disease was identified in a child presenting with psychomotor
      retardation, hypotonia, growth retardation, dysmorphic features and
      anorexia.
    explanation: >-
      The original report describes growth retardation and anorexia in an
      ALG12-CDG child.
  - reference: PMID:38717015
    reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Currently reported features of ALG12-CDG include: developmental delay,
      hypotonia, failure to thrive and/or short stature, brain anomalies,
      recurrent infections, hypogammaglobulinemia, coagulation abnormalities,
      and genitourinary abnormalities.
    explanation: >-
      The prenatal phenotype report summarizes failure to thrive and/or short
      stature in the broader ALG12-CDG phenotype.
  downstream:
  - target: Failure to thrive
    description: Failure to thrive is a reported growth manifestation of ALG12-CDG.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:38717015
      reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Currently reported features of ALG12-CDG include: developmental delay,
        hypotonia, failure to thrive and/or short stature, brain anomalies,
        recurrent infections, hypogammaglobulinemia, coagulation abnormalities,
        and genitourinary abnormalities.
      explanation: The report lists failure to thrive among ALG12-CDG features.
  - target: Short stature
    description: Short stature is a reported growth manifestation of ALG12-CDG.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:38717015
      reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Currently reported features of ALG12-CDG include: developmental delay,
        hypotonia, failure to thrive and/or short stature, brain anomalies,
        recurrent infections, hypogammaglobulinemia, coagulation abnormalities,
        and genitourinary abnormalities.
      explanation: The report lists short stature as a growth manifestation.
- name: Dysmorphic and genitourinary developmental involvement
  description: >-
    ALG12-CDG can involve craniofacial dysmorphism and male genitourinary
    developmental abnormalities.
  evidence:
  - reference: PMID:31481313
    reference_title: "Complex phenotypes in ALG12-congenital disorder of glycosylation (ALG12-CDG): Case series and review of the literature."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare disorder
      caused by a deficiency of
      dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase which
      presents with intellectual disability, hypotonia, dysmorphic features, low
      IgG levels with recurrent infections, male genital hypoplasia, and
      coagulation abnormalities.
    explanation: >-
      The case-series abstract lists dysmorphic features and male genital
      hypoplasia in ALG12-CDG.
  - reference: CGGV:assertion_21631e85-8133-4703-a77c-c046effb6e56-2023-02-15T200000.000Z
    reference_title: "ALG12 / ALG12-congenital disorder of glycosylation (Definitive)"
    supports: SUPPORT
    evidence_source: OTHER
    snippet: >-
      Common phenotypes include intellectual disability, hypotonia, dysmorphic
      features, coagulation abnormalities and genitourinary abnormalities.
    explanation: >-
      ClinGen independently lists dysmorphic and genitourinary abnormalities as
      common ALG12-CDG phenotypes.
  downstream:
  - target: Facial dysmorphism
    description: Dysmorphic features include craniofacial involvement.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:31481313
      reference_title: "Complex phenotypes in ALG12-congenital disorder of glycosylation (ALG12-CDG): Case series and review of the literature."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare
        disorder caused by a deficiency of
        dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase
        which presents with intellectual disability, hypotonia, dysmorphic
        features, low IgG levels with recurrent infections, male genital
        hypoplasia, and coagulation abnormalities.
      explanation: The case-series abstract lists dysmorphic features.
  - target: Hypoplastic male external genitalia
    description: Male genital hypoplasia is a genitourinary developmental manifestation.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:31481313
      reference_title: "Complex phenotypes in ALG12-congenital disorder of glycosylation (ALG12-CDG): Case series and review of the literature."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare
        disorder caused by a deficiency of
        dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase
        which presents with intellectual disability, hypotonia, dysmorphic
        features, low IgG levels with recurrent infections, male genital
        hypoplasia, and coagulation abnormalities.
      explanation: The case-series abstract lists male genital hypoplasia.
- name: Renal and skeletal developmental involvement
  description: >-
    Severe ALG12-CDG can involve prenatal renal malformation and
    skeletal-dysplasia-like abnormalities.
  locations:
  - preferred_term: kidney
    term:
      id: UBERON:0002113
      label: kidney
  - preferred_term: skeletal system
    term:
      id: UBERON:0001434
      label: skeletal system
  biological_processes:
  - preferred_term: kidney development
    modifier: ABNORMAL
    term:
      id: GO:0001822
      label: kidney development
  - preferred_term: skeletal system development
    modifier: ABNORMAL
    term:
      id: GO:0001501
      label: skeletal system development
  evidence:
  - reference: PMID:38717015
    reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      In addition, skeletal abnormalities resembling a skeletal dysplasia
      including shortened long bones and talipes equinovarus have been seen in
      more severe neonatal presentation of this disorder.
    explanation: >-
      The prenatal report supports skeletal-dysplasia-like abnormalities in
      severe neonatal ALG12-CDG.
  - reference: PMID:38717015
    reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      We report on a case expanding the phenotype of ALG12-CDG to include
      bilateral, multicystic kidneys in a neonatal demise identified with
      homozygous pathogenic variants in the ALG12 gene at c.1001del
      (p.N334Tfs*15) through clinical trio exome sequencing.
    explanation: >-
      The case report directly supports multicystic renal malformation in a
      severe prenatal ALG12-CDG presentation.
  downstream:
  - target: Skeletal dysplasia
    description: Skeletal dysplasia-like abnormalities are part of the severe neonatal branch.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:38717015
      reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        In addition, skeletal abnormalities resembling a skeletal dysplasia
        including shortened long bones and talipes equinovarus have been seen in
        more severe neonatal presentation of this disorder.
      explanation: The report directly supports skeletal dysplasia-like abnormalities.
  - target: Multicystic kidney dysplasia
    description: Multicystic kidneys expand the renal phenotype of severe ALG12-CDG.
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    evidence:
    - reference: PMID:38717015
      reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        We report on a case expanding the phenotype of ALG12-CDG to include
        bilateral, multicystic kidneys in a neonatal demise identified with
        homozygous pathogenic variants in the ALG12 gene at c.1001del
        (p.N334Tfs*15) through clinical trio exome sequencing.
      explanation: The case report directly supports multicystic kidney dysplasia.
- name: Adult cardioskeletal structural involvement
  description: >-
    A mild adult ALG12-CDG presentation included ventricular septal defect and
    severe scoliosis; causal directness and possible modifier contributions are
    unresolved.
  locations:
  - preferred_term: heart
    term:
      id: UBERON:0000948
      label: heart
  - preferred_term: skeletal system
    term:
      id: UBERON:0001434
      label: skeletal system
  evidence:
  - reference: PMID:32530140
    reference_title: "ALG12-CDG: An unusual patient without intellectual disability and facial dysmorphism, and with a novel variant."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      We here present an intriguing patient with an exceptional phenotype:
      25-year-old women with a ventricular septal defect and severe idiopathic
      scoliosis but no facial dysmorphism, who dances as a professional, and has
      a University degree.
    explanation: >-
      The adult case report supports the reported co-occurrence of VSD and
      severe scoliosis in a genetically diagnosed ALG12-CDG patient.
  downstream:
  - target: Ventricular septal defect
    description: Ventricular septal defect was reported in the mild adult ALG12-CDG case.
    causal_link_type: UNKNOWN
    evidence:
    - reference: PMID:32530140
      reference_title: "ALG12-CDG: An unusual patient without intellectual disability and facial dysmorphism, and with a novel variant."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        We here present an intriguing patient with an exceptional phenotype:
        25-year-old women with a ventricular septal defect and severe idiopathic
        scoliosis but no facial dysmorphism, who dances as a professional, and
        has a University degree.
      explanation: The case report directly identifies ventricular septal defect.
  - target: Scoliosis
    description: Severe scoliosis was reported in the mild adult ALG12-CDG case.
    causal_link_type: UNKNOWN
    evidence:
    - reference: PMID:32530140
      reference_title: "ALG12-CDG: An unusual patient without intellectual disability and facial dysmorphism, and with a novel variant."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        We here present an intriguing patient with an exceptional phenotype:
        25-year-old women with a ventricular septal defect and severe idiopathic
        scoliosis but no facial dysmorphism, who dances as a professional, and
        has a University degree.
      explanation: The case report directly identifies severe scoliosis.
phenotypes:
- name: Global developmental delay
  category: Neurologic
  description: Developmental delay is a reported core feature of ALG12-CDG.
  phenotype_term:
    preferred_term: Global developmental delay
    term:
      id: HP:0001263
      label: Global developmental delay
  evidence:
  - reference: PMID:38717015
    reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Currently reported features of ALG12-CDG include: developmental delay,
      hypotonia, failure to thrive and/or short stature, brain anomalies,
      recurrent infections, hypogammaglobulinemia, coagulation abnormalities,
      and genitourinary abnormalities.
    explanation: >-
      The prenatal phenotype report summarizes developmental delay among
      reported ALG12-CDG features.
- name: Intellectual disability
  category: Neurologic
  description: Intellectual disability is a core neurodevelopmental phenotype in ALG12-CDG.
  phenotype_term:
    preferred_term: Intellectual disability
    term:
      id: HP:0001249
      label: Intellectual disability
  evidence:
  - reference: PMID:31481313
    reference_title: "Complex phenotypes in ALG12-congenital disorder of glycosylation (ALG12-CDG): Case series and review of the literature."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare disorder
      caused by a deficiency of
      dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase which
      presents with intellectual disability, hypotonia, dysmorphic features, low
      IgG levels with recurrent infections, male genital hypoplasia, and
      coagulation abnormalities.
    explanation: >-
      The case-series abstract directly lists intellectual disability among
      ALG12-CDG presenting features.
  - reference: CGGV:assertion_21631e85-8133-4703-a77c-c046effb6e56-2023-02-15T200000.000Z
    reference_title: "ALG12 / ALG12-congenital disorder of glycosylation (Definitive)"
    supports: SUPPORT
    evidence_source: OTHER
    snippet: >-
      Common phenotypes include intellectual disability, hypotonia, dysmorphic
      features, coagulation abnormalities and genitourinary abnormalities.
    explanation: >-
      ClinGen names intellectual disability as a common phenotype for this
      gene-disease assertion.
- name: Generalized hypotonia
  category: Neurologic
  description: Generalized hypotonia is a recurrent neurologic manifestation.
  phenotype_term:
    preferred_term: Generalized hypotonia
    term:
      id: HP:0001290
      label: Generalized hypotonia
  evidence:
  - reference: PMID:38717015
    reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Currently reported features of ALG12-CDG include: developmental delay,
      hypotonia, failure to thrive and/or short stature, brain anomalies,
      recurrent infections, hypogammaglobulinemia, coagulation abnormalities,
      and genitourinary abnormalities.
    explanation: The report lists hypotonia as a currently reported feature.
- name: Failure to thrive
  category: Growth
  description: Feeding and growth impairment can present as failure to thrive.
  phenotype_term:
    preferred_term: Failure to thrive
    term:
      id: HP:0001508
      label: Failure to thrive
  evidence:
  - reference: PMID:38717015
    reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Currently reported features of ALG12-CDG include: developmental delay,
      hypotonia, failure to thrive and/or short stature, brain anomalies,
      recurrent infections, hypogammaglobulinemia, coagulation abnormalities,
      and genitourinary abnormalities.
    explanation: The report lists failure to thrive among current features.
- name: Short stature
  category: Growth
  description: Short stature is part of the reported growth phenotype.
  phenotype_term:
    preferred_term: Short stature
    term:
      id: HP:0004322
      label: Short stature
  evidence:
  - reference: PMID:38717015
    reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Currently reported features of ALG12-CDG include: developmental delay,
      hypotonia, failure to thrive and/or short stature, brain anomalies,
      recurrent infections, hypogammaglobulinemia, coagulation abnormalities,
      and genitourinary abnormalities.
    explanation: The report lists short stature as an alternate growth manifestation.
- name: Recurrent respiratory infections
  category: Immune
  description: Recurrent respiratory infections reflect immune involvement.
  phenotype_term:
    preferred_term: Recurrent respiratory infections
    term:
      id: HP:0002205
      label: Recurrent respiratory infections
  evidence:
  - reference: PMID:38717015
    reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Currently reported features of ALG12-CDG include: developmental delay,
      hypotonia, failure to thrive and/or short stature, brain anomalies,
      recurrent infections, hypogammaglobulinemia, coagulation abnormalities,
      and genitourinary abnormalities.
    explanation: >-
      The case-series abstract explicitly links low IgG levels with recurrent
      infections.
- name: Facial dysmorphism
  category: Craniofacial
  description: Dysmorphic facial features are a common craniofacial manifestation.
  phenotype_term:
    preferred_term: Facial dysmorphism
    term:
      id: HP:0001999
      label: Abnormal facial shape
  evidence:
  - reference: PMID:31481313
    reference_title: "Complex phenotypes in ALG12-congenital disorder of glycosylation (ALG12-CDG): Case series and review of the literature."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare disorder
      caused by a deficiency of
      dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase which
      presents with intellectual disability, hypotonia, dysmorphic features, low
      IgG levels with recurrent infections, male genital hypoplasia, and
      coagulation abnormalities.
    explanation: >-
      The case-series abstract explicitly lists dysmorphic features in
      ALG12-CDG.
  - reference: CGGV:assertion_21631e85-8133-4703-a77c-c046effb6e56-2023-02-15T200000.000Z
    reference_title: "ALG12 / ALG12-congenital disorder of glycosylation (Definitive)"
    supports: SUPPORT
    evidence_source: OTHER
    snippet: >-
      Common phenotypes include intellectual disability, hypotonia, dysmorphic
      features, coagulation abnormalities and genitourinary abnormalities.
    explanation: >-
      ClinGen independently lists dysmorphic features as common in ALG12-CDG.
- name: Decreased circulating immunoglobulin concentration
  category: Immune
  description: Hypogammaglobulinemia or low serum immunoglobulins can occur.
  phenotype_term:
    preferred_term: Hypogammaglobulinemia
    term:
      id: HP:0004313
      label: Decreased circulating immunoglobulin concentration
  evidence:
  - reference: PMID:31529350
    reference_title: "ALG12-CDG: novel glycophenotype insights endorse the molecular defect."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      ALG12-CDG is a severe multisystem disease associated with low to
      deficient serum immunoglobulins and recurrent infections.
    explanation: The glycophenotype report directly supports low serum immunoglobulins.
- name: Abnormality of coagulation
  category: Hematologic
  description: ALG12-CDG can involve coagulation factor abnormalities.
  phenotype_term:
    preferred_term: Coagulation abnormalities
    term:
      id: HP:0001928
      label: Abnormality of coagulation
  evidence:
  - reference: PMID:38717015
    reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Currently reported features of ALG12-CDG include: developmental delay,
      hypotonia, failure to thrive and/or short stature, brain anomalies,
      recurrent infections, hypogammaglobulinemia, coagulation abnormalities,
      and genitourinary abnormalities.
    explanation: The case-series abstract lists coagulation abnormalities.
  - reference: PMID:32530140
    reference_title: "ALG12-CDG: An unusual patient without intellectual disability and facial dysmorphism, and with a novel variant."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Increased levels of hypoglycosylated forms of F XI (also with significant
      deficiency) and transferrin were also detected.
    explanation: >-
      The adult case demonstrates hypoglycosylation and deficiency of a
      coagulation factor.
- name: Hypoplastic male external genitalia
  category: Genitourinary
  description: Male genital hypoplasia is part of the reported ALG12-CDG genitourinary spectrum.
  phenotype_term:
    preferred_term: Male genital hypoplasia
    term:
      id: HP:0000050
      label: Hypoplastic male external genitalia
  evidence:
  - reference: PMID:31481313
    reference_title: "Complex phenotypes in ALG12-congenital disorder of glycosylation (ALG12-CDG): Case series and review of the literature."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare disorder
      caused by a deficiency of
      dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase which
      presents with intellectual disability, hypotonia, dysmorphic features, low
      IgG levels with recurrent infections, male genital hypoplasia, and
      coagulation abnormalities.
    explanation: >-
      The Tahata case-series abstract directly lists male genital hypoplasia.
  - reference: CGGV:assertion_21631e85-8133-4703-a77c-c046effb6e56-2023-02-15T200000.000Z
    reference_title: "ALG12 / ALG12-congenital disorder of glycosylation (Definitive)"
    supports: SUPPORT
    evidence_source: OTHER
    snippet: >-
      Common phenotypes include intellectual disability, hypotonia, dysmorphic
      features, coagulation abnormalities and genitourinary abnormalities.
    explanation: >-
      ClinGen supports genitourinary abnormalities as common in ALG12-CDG.
- name: Skeletal dysplasia
  category: Musculoskeletal
  description: Severe neonatal presentations can include skeletal dysplasia-like anomalies.
  phenotype_term:
    preferred_term: Skeletal dysplasia
    term:
      id: HP:0002652
      label: Skeletal dysplasia
  evidence:
  - reference: PMID:38717015
    reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      In addition, skeletal abnormalities resembling a skeletal dysplasia
      including shortened long bones and talipes equinovarus have been seen in
      more severe neonatal presentation of this disorder.
    explanation: >-
      The prenatal phenotype report directly supports a skeletal
      dysplasia-like phenotype in severe neonatal ALG12-CDG.
- name: Multicystic kidney dysplasia
  category: Renal
  description: Bilateral multicystic kidneys have been reported as an expanded prenatal phenotype.
  phenotype_term:
    preferred_term: Multicystic kidney dysplasia
    term:
      id: HP:0000003
      label: Multicystic kidney dysplasia
  evidence:
  - reference: PMID:38717015
    reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      We report on a case expanding the phenotype of ALG12-CDG to include
      bilateral, multicystic kidneys in a neonatal demise identified with
      homozygous pathogenic variants in the ALG12 gene at c.1001del
      (p.N334Tfs*15) through clinical trio exome sequencing.
    explanation: >-
      This directly supports multicystic kidneys as a reported prenatal
      extension of the phenotype.
- name: Ventricular septal defect
  category: Cardiovascular
  description: Ventricular septal defect has been reported in an unusually mild adult ALG12-CDG case.
  phenotype_term:
    preferred_term: Ventricular septal defect
    term:
      id: HP:0001629
      label: Ventricular septal defect
  evidence:
  - reference: PMID:32530140
    reference_title: "ALG12-CDG: An unusual patient without intellectual disability and facial dysmorphism, and with a novel variant."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      We here present an intriguing patient with an exceptional phenotype:
      25-year-old women with a ventricular septal defect and severe idiopathic
      scoliosis but no facial dysmorphism, who dances as a professional, and
      has a University degree.
    explanation: >-
      The adult case report directly identifies ventricular septal defect in an
      ALG12-CDG patient.
- name: Scoliosis
  category: Musculoskeletal
  description: Scoliosis has been reported in a mild adult presentation.
  phenotype_term:
    preferred_term: Scoliosis
    term:
      id: HP:0002650
      label: Scoliosis
  evidence:
  - reference: PMID:32530140
    reference_title: "ALG12-CDG: An unusual patient without intellectual disability and facial dysmorphism, and with a novel variant."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      We here present an intriguing patient with an exceptional phenotype:
      25-year-old women with a ventricular septal defect and severe idiopathic
      scoliosis but no facial dysmorphism, who dances as a professional, and
      has a University degree.
    explanation: >-
      The adult case report directly identifies severe scoliosis in an
      ALG12-CDG patient.
biochemical:
- name: Type I transferrin hypoglycosylation pattern
  presence: Present
  specificity: Diagnostic support
  biomarker_term:
    preferred_term: N-glycan
    term:
      id: CHEBI:59520
      label: N-glycan
  readouts:
  - target: N-glycan precursor formation defect
    relationship: READOUT_OF
    direction: PRESENT_ABSENT
    endpoint_context: DIAGNOSTIC
    interpretation: >-
      A type I transferrin hypoglycosylation pattern reports impaired
      N-glycosylation-site occupancy downstream of the ALG12 precursor defect.
  evidence:
  - reference: PMID:34467644
    reference_title: A novel homozygous mutation in the human ALG12 gene results in an aberrant profile of oligomannose N-glycans in patient's serum.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Due to a newborn Slovak patient's clinical and biochemical abnormalities,
      the isoelectric focusing of transferrin was performed with observed
      significant hypoglycosylation typical of CDG I.
    explanation: >-
      This directly supports transferrin isoelectric focusing as a biochemical
      marker of type I CDG in ALG12-CDG.
- name: Aberrant serum oligomannose N-glycan profile
  presence: Present
  specificity: Diagnostic support
  biomarker_term:
    preferred_term: N-glycan
    term:
      id: CHEBI:59520
      label: N-glycan
  readouts:
  - target: ALG12 mannosyltransferase deficiency
    relationship: READOUT_OF
    direction: PRESENT_ABSENT
    endpoint_context: DIAGNOSTIC
    interpretation: >-
      Accumulation of GlcNAc2Man5-7 with reduced GlcNAc2Man8-9 reports impaired
      ALG12 enzymatic activity.
  evidence:
  - reference: PMID:34467644
    reference_title: A novel homozygous mutation in the human ALG12 gene results in an aberrant profile of oligomannose N-glycans in patient's serum.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Furthermore, analysis of neutral serum N-glycans by mass spectrometry
      revealed the accumulation of GlcNAc2Man5-7 and decreased levels of
      GlcNAc2Man8-9, which indicated impaired ALG12 enzymatic activity.
    explanation: >-
      Serum N-glycan profiling directly supports an ALG12-associated
      oligomannose signature.
- name: Hypoglycosylated antithrombin and factor XI
  presence: Present
  specificity: Supportive
  readouts:
  - target: Abnormal serum and IgG N-glycosylation
    relationship: READOUT_OF
    direction: PRESENT_ABSENT
    endpoint_context: DIAGNOSTIC
    interpretation: >-
      Hypoglycosylated coagulation proteins report systemic plasma-protein
      hypoglycosylation caused by the ALG12-CDG glycosylation defect.
  evidence:
  - reference: PMID:32530140
    reference_title: "ALG12-CDG: An unusual patient without intellectual disability and facial dysmorphism, and with a novel variant."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Congenital disorder of glycosylation diagnosis started through the
      identification of antithrombin deficiency without SERPINC1 defect and the
      detection of hypoglycosylated forms.
    explanation: >-
      The adult case shows a coagulation-protein hypoglycosylation clue to
      diagnosis.
genetic:
- name: ALG12
  association: Biallelic loss-of-function variants
  relationship_type: CAUSATIVE
  variant_origin: GERMLINE
  gene_term:
    preferred_term: ALG12
    term:
      id: hgnc:19358
      label: ALG12
  evidence:
  - reference: PMID:34467644
    reference_title: A novel homozygous mutation in the human ALG12 gene results in an aberrant profile of oligomannose N-glycans in patient's serum.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Genetic analysis of the coding regions of the ALG12 gene of the patient
      revealed a novel homozygous substitution mutation c.1439T>C
      p.(Leu480Pro) within Exon 10.
    explanation: >-
      The case report documents a homozygous ALG12 variant in an affected
      patient.
  - reference: PMID:38717015
    reference_title: Expanded prenatal phenotype of ALG12-associated congenital disorder of glycosylation including bilateral multicystic kidneys.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      We report on a case expanding the phenotype of ALG12-CDG to include
      bilateral, multicystic kidneys in a neonatal demise identified with
      homozygous pathogenic variants in the ALG12 gene at c.1001del
      (p.N334Tfs*15) through clinical trio exome sequencing.
    explanation: >-
      The prenatal case report identifies a homozygous pathogenic ALG12
      frameshift variant.
  - reference: CGGV:assertion_21631e85-8133-4703-a77c-c046effb6e56-2023-02-15T200000.000Z
    reference_title: "ALG12 / ALG12-congenital disorder of glycosylation (Definitive)"
    supports: SUPPORT
    evidence_source: OTHER
    snippet: >-
      In summary, there is definitive evidence to support the relationship
      between ALG12 and autosomal recessive ALG12 -congenital disorder of
      glycosylation.
    explanation: >-
      ClinGen provides a deterministic structured assertion for the ALG12
      gene-disease relationship.
diagnosis:
- name: Transferrin isoelectric focusing and serum glycomics
  description: >-
    Biochemical evaluation uses transferrin isoelectric focusing and serum
    N-glycan profiling to detect a type I CDG pattern and ALG12-associated
    oligomannose abnormalities.
  diagnosis_term:
    preferred_term: clinical laboratory procedure
    term:
      id: MAXO:0000006
      label: clinical laboratory procedure
  results: >-
    Type I transferrin hypoglycosylation with accumulation of GlcNAc2Man5-7 and
    decreased GlcNAc2Man8-9 supports ALG12-CDG.
  evidence:
  - reference: PMID:34467644
    reference_title: A novel homozygous mutation in the human ALG12 gene results in an aberrant profile of oligomannose N-glycans in patient's serum.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Due to a newborn Slovak patient's clinical and biochemical abnormalities,
      the isoelectric focusing of transferrin was performed with observed
      significant hypoglycosylation typical of CDG I.
    explanation: >-
      This supports transferrin isoelectric focusing as first-line biochemical
      evidence of a type I CDG pattern.
  - reference: PMID:34467644
    reference_title: A novel homozygous mutation in the human ALG12 gene results in an aberrant profile of oligomannose N-glycans in patient's serum.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Furthermore, analysis of neutral serum N-glycans by mass spectrometry
      revealed the accumulation of GlcNAc2Man5-7 and decreased levels of
      GlcNAc2Man8-9, which indicated impaired ALG12 enzymatic activity.
    explanation: >-
      This supports serum N-glycan mass spectrometry for resolving the
      ALG12-associated glycomic signature.
- name: ALG12 molecular genetic testing
  description: >-
    Molecular testing confirms ALG12-CDG by identifying biallelic pathogenic
    ALG12 variants in a compatible biochemical and clinical context.
  diagnosis_term:
    preferred_term: molecular genetic testing
    term:
      id: MAXO:0000533
      label: molecular genetic testing
  results: >-
    Homozygous or compound heterozygous pathogenic ALG12 variants confirm the
    diagnosis when paired with compatible glycosylation abnormalities.
  evidence:
  - reference: PMID:34467644
    reference_title: A novel homozygous mutation in the human ALG12 gene results in an aberrant profile of oligomannose N-glycans in patient's serum.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      This comprehensive genomic and glycomic approach led to the confirmation
      of the ALG12 pathogenic variant responsible for the clinical manifestation
      of the disorder in the patient described.
    explanation: >-
      The case report directly supports combined genomic and glycomic
      confirmation of the diagnosis.
  - reference: PMID:37644541
    reference_title: "Congenital disorders of glycosylation: narration of a story through its patents."
    supports: PARTIAL
    evidence_source: OTHER
    snippet: >-
      CDG diagnosis has been at a rapid pace since the introduction of
      whole-exome/whole-genome sequencing as a diagnostic tool.
    explanation: >-
      This CDG-wide review supports genome-scale sequencing as a modern
      diagnostic approach for CDG; it is broader than ALG12-CDG alone.
treatments:
- name: Supportive multidisciplinary care
  description: >-
    No ALG12-CDG-specific disease-modifying therapy is established in the
    fetched evidence; care is supportive and directed to infections,
    nutrition/growth, neurodevelopmental needs, coagulation abnormalities, and
    organ-specific complications.
  treatment_term:
    preferred_term: Supportive Care
    term:
      id: NCIT:C15747
      label: Supportive Care
  target_phenotypes:
  - preferred_term: Recurrent respiratory infections
    term:
      id: HP:0002205
      label: Recurrent respiratory infections
  - preferred_term: Coagulation abnormalities
    term:
      id: HP:0001928
      label: Abnormality of coagulation
  evidence:
  - reference: PMID:37644541
    reference_title: "Congenital disorders of glycosylation: narration of a story through its patents."
    supports: PARTIAL
    evidence_source: OTHER
    snippet: >-
      Since the first clinical report in 1980 of PMM2-CDG, the most common CDG
      worldwide, research made great strides, but nearly all of them are still
      missing a cure.
    explanation: >-
      This CDG-wide review supports the lack of curative therapy for most CDGs;
      by itself it only partially supports ALG12-CDG-specific supportive care.
- name: IVIG/SCIG immunoglobulin replacement therapy
  description: >-
    Intravenous or subcutaneous immunoglobulin replacement is a disease-specific
    supportive consideration for ALG12-CDG patients with low immunoglobulins and
    recurrent infections. Validator-cache evidence supports the
    antibody-deficiency indication, while direct published treatment-use details
    are limited.
  treatment_term:
    preferred_term: immunoglobulin infusion therapy
    term:
      id: MAXO:0001480
      label: immunoglobulin infusion therapy
    therapeutic_agent:
    - preferred_term: Therapeutic Immune Globulin
      term:
        id: NCIT:C2701
        label: Therapeutic Immune Globulin
  target_phenotypes:
  - preferred_term: Hypogammaglobulinemia
    term:
      id: HP:0004313
      label: Decreased circulating immunoglobulin concentration
  - preferred_term: Recurrent respiratory infections
    term:
      id: HP:0002205
      label: Recurrent respiratory infections
  evidence:
  - reference: PMID:31529350
    reference_title: "ALG12-CDG: novel glycophenotype insights endorse the molecular defect."
    supports: PARTIAL
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      ALG12-CDG is a severe multisystem disease associated with low to
      deficient serum immunoglobulins and recurrent infections.
    explanation: >-
      This supports the hypogammaglobulinemia and recurrent-infection
      indication for immunoglobulin replacement; it does not by itself document
      treatment response.
  - reference: PMID:31481313
    reference_title: "Complex phenotypes in ALG12-congenital disorder of glycosylation (ALG12-CDG): Case series and review of the literature."
    supports: PARTIAL
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      ALG12-congenital disorder of glycosylation (ALG12-CDG) is a rare disorder
      caused by a deficiency of
      dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase which
      presents with intellectual disability, hypotonia, dysmorphic features, low
      IgG levels with recurrent infections, male genital hypoplasia, and
      coagulation abnormalities.
    explanation: >-
      The case series supports the low-IgG and recurrent-infection phenotype
      targeted by immunoglobulin replacement, but the cached abstract does not
      directly quote treatment use.
clinical_trials:
- name: NCT04199000
  status: RECRUITING
  description: >-
    CDG-wide observational natural-history study relevant to genetically,
    enzymatically, or molecularly confirmed CDG subtypes such as ALG12-CDG.
  evidence:
  - reference: clinicaltrials:NCT04199000
    reference_title: Clinical and Basic Investigations Into Congenital Disorders of Glycosylation
    supports: PARTIAL
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The purpose of this research is to study the natural history of
      congenital disorders of glycosylation and its causes and treatments.
    explanation: >-
      The study is CDG-wide rather than ALG12-specific, so it partially
      supports relevance to ALG12-CDG natural-history research.
notes: >-
  Falcon deep research completed on 2026-05-10. The 2002 original ALG12-CDG
  abstract was fetched as PMID:12217961, and this curation uses fetched PubMed,
  ClinGen, and ClinicalTrials.gov caches with validator-checkable snippets.
datasets: []
📚

References & Deep Research

Deep Research

1
Falcon
ALG12–Congenital Disorder of Glycosylation (ALG12‑CDG; historical CDG‑Ig) — Disease Characteristics Research Report
Edison Scientific Literature 35 citations 2026-05-10T14:56:01.118066

ALG12–Congenital Disorder of Glycosylation (ALG12‑CDG; historical CDG‑Ig) — Disease Characteristics Research Report

Executive summary

ALG12‑CDG is a very rare autosomal recessive congenital disorder of glycosylation caused by deficiency of the ER luminal α1,6‑mannosyltransferase ALG12, which catalyzes addition of the 8th mannose during lipid‑linked oligosaccharide (LLO) assembly for protein N‑glycosylation. Biochemically it produces a type‑I carbohydrate‑deficient transferrin pattern and characteristic accumulation of truncated LLO/N‑glycans (notably Man7 intermediates and reduced Man8/Man9 species), with multisystem disease that often includes neurodevelopmental impairment, growth failure, dysmorphism, immunodeficiency (hypogammaglobulinemia), coagulation abnormalities, and variable severity up to early death. Recent (2023–2024) literature emphasizes accelerated CDG diagnosis via WES/WGS and emerging multi‑omics/proteomics tools, but also highlights the ongoing lack of targeted therapies for most CDGs, including ALG12‑CDG. (grubenmann2002alg12mannosyltransferasedefect pages 1-2, grubenmann2002alg12mannosyltransferasedefect pages 2-3, ziburova2021anovelhomozygous pages 1-1, monticelli2023congenitaldisordersof pages 1-2, pascoal2024revisitingtheimmunopathology pages 4-6)

Abbreviations

CDG: congenital disorder(s) of glycosylation; ER: endoplasmic reticulum; LLO: lipid‑linked oligosaccharide; Tf IEF: transferrin isoelectric focusing; MALDI‑TOF: matrix‑assisted laser desorption/ionization time‑of‑flight; MS: mass spectrometry; WES/WGS: whole‑exome/whole‑genome sequencing.


1. Disease information

1.1 What is the disease?

The first molecularly defined report described “a deficiency in the ALG12 ER α1,6‑mannosyltransferase resulting in a novel type of glycosylation disorder” and explicitly stated: “The ALG12 mannosyltransferase defect defines a new type of congenital disorder of glycosylation, designated CDG‑Ig.” (Grubenmann et al., 2002‑09; Human Molecular Genetics; DOI https://doi.org/10.1093/hmg/11.19.2331) (grubenmann2002alg12mannosyltransferasedefect pages 1-2)

A later case report defined the entity as: “Congenital disorder of glycosylation type Ig (ALG12‑CDG) is a rare inherited metabolic disease caused by a defect in alpha‑mannosyltransferase 8, encoded by the ALG12 gene (22q13.33).” (Ziburová et al., 2021‑09; American Journal of Medical Genetics A; DOI https://doi.org/10.1002/ajmg.a.62474) (ziburova2021anovelhomozygous pages 1-1)

1.2 Key identifiers

  • MONDO: MONDO_0011783 (“ALG12‑congenital disorder of glycosylation”) (OpenTargets disease identifier) (OpenTargets Search: ALG12-congenital disorder of glycosylation-ALG12)
  • OMIM/MIM: Literature excerpts report #607143 (ziburova2021anovelhomozygous pages 2-2, sturiale2019alg12cdgnovelglycophenotype pages 1-2); one excerpt reported OMIM: 607144 (tahata2019complexphenotypesin pages 1-2). These inconsistencies indicate that a direct OMIM lookup is recommended for knowledge‑base normalization.
  • Orphanet / ICD‑10 / ICD‑11 / MeSH: Not present in the retrieved full texts; would require direct lookup in those databases (limitation of current tool‑retrieved corpus).

1.3 Synonyms / alternative names

  • ALG12‑CDG (ziburova2021anovelhomozygous pages 1-1)
  • ALG12‑congenital disorder of glycosylation (ziburova2021anovelhomozygous pages 7-8)
  • CDG‑Ig (historical) (grubenmann2002alg12mannosyltransferasedefect pages 1-2)
  • “ALG12 mannosyltransferase defect” / “ALG12 α1,6‑mannosyltransferase deficiency” (functional descriptor) (grubenmann2002alg12mannosyltransferasedefect pages 1-2, sturiale2019alg12cdgnovelglycophenotype pages 1-2)

1.4 Evidence sources

Most disease‑specific information is derived from individual patient case reports/series plus aggregated reviews of CDG (e.g., immunopathology and diagnostic evolution). (grubenmann2002alg12mannosyltransferasedefect pages 1-2, tahata2019complexphenotypesin pages 1-2, monticelli2023congenitaldisordersof pages 1-2, pascoal2024revisitingtheimmunopathology pages 4-6)


2. Etiology

2.1 Disease causal factors

Primary cause (genetic): biallelic pathogenic variants in ALG12, encoding an ER mannosyltransferase involved in N‑glycan precursor assembly. (ziburova2021anovelhomozygous pages 2-2, grubenmann2002alg12mannosyltransferasedefect pages 1-2)

Mechanistic definition from a 2021 report: ALG12 encodes “the dolichyl‑P‑mannose Man‑7‑GlcNAc‑2‑PP‑dolichyl‑alpha‑6‑mannosyltransferase,” and “this enzyme transfers the eighth mannose residue from dolichyl‑P‑mannose to lipid‑linked oligosaccharides.” (Ziburová et al., 2021‑09; DOI https://doi.org/10.1002/ajmg.a.62474) (ziburova2021anovelhomozygous pages 2-2)

2.2 Risk factors

  • Genetic risk factors (causal variants): autosomal‑recessive inheritance; risk elevated in families with carrier parents and potentially consanguinity (not directly documented in retrieved excerpts). (ziburova2021anovelhomozygous pages 2-2)
  • Environmental risk factors: none established for causation in current evidence; clinical complications (e.g., infections) are downstream consequences.

2.3 Protective factors

No validated protective genetic or environmental factors were identified in the retrieved evidence.

2.4 Gene–environment interactions

Not specifically described for ALG12‑CDG in the retrieved evidence. In CDG more broadly, host glycan alterations may influence host–pathogen interactions, but ALG12‑specific GxE data were not retrieved. (pascoal2024revisitingtheimmunopathology pages 1-2)


3. Phenotypes (clinical features)

ALG12‑CDG presents as a multisystem disorder with marked inter‑individual variability.

3.1 Core phenotype spectrum (human evidence)

  • Neurodevelopmental: psychomotor delay/intellectual disability and hypotonia are prominent; the index case had “psychomotor retardation” and “hypotonia.” (grubenmann2002alg12mannosyltransferasedefect pages 1-2)
  • Growth / nutrition: growth retardation/failure to thrive, feeding problems/anorexia. (grubenmann2002alg12mannosyltransferasedefect pages 1-2, ziburova2021anovelhomozygous pages 5-5)
  • Craniofacial / dysmorphism: characteristic dysmorphism described across cases. (grubenmann2002alg12mannosyltransferasedefect pages 1-2, ziburova2021anovelhomozygous pages 5-5)
  • Immunologic / infection susceptibility: low immunoglobulins and recurrent infections; the index case had “markedly low immunoglobulins” and recurrent pneumonias requiring immunoglobulin infusions. (grubenmann2002alg12mannosyltransferasedefect pages 2-3)
  • Coagulation: low antithrombin and other coagulation factor abnormalities; e.g., low antithrombin reported in multiple cases. (grubenmann2002alg12mannosyltransferasedefect pages 2-3, sturiale2019alg12cdgnovelglycophenotype pages 6-8)
  • Genitourinary / endocrine‑related: male genital anomalies such as “micropenis” and undescended testes in the original case. (grubenmann2002alg12mannosyltransferasedefect pages 2-3)
  • Skeletal: skeletal abnormalities reported as frequent across published cases. (ziburova2021anovelhomozygous pages 5-5)

A 2021 synthesis of published cases reported frequent features including: “characteristic dysmorphism, psychomotor retardation, hypotonia, and/or skeletal abnormalities,” and also noted “feeding difficulties, respiratory distress, and frequent infections.” (Ziburová et al., 2021‑09; DOI https://doi.org/10.1002/ajmg.a.62474) (ziburova2021anovelhomozygous pages 5-5)

3.2 Age of onset, severity, progression

  • Onset is typically congenital/neonatal or early infancy, consistent with CDG type I disorders; a 2021 report describes evaluation in a “newborn” with significant biochemical abnormalities. (ziburova2021anovelhomozygous pages 1-1)
  • Severity is variable, ranging from severe infantile lethal multisystem disease to milder presentations; a 2019 case series described three brothers including one who “died at 18 months” and two survivors with comparatively milder disease. (tahata2019complexphenotypesin pages 1-2)

3.3 Quality of life impact

Direct QoL instrument data specific to ALG12‑CDG were not captured in the retrieved texts. Real‑world impact is inferred from multisystem disability (developmental delay, recurrent infections, feeding problems).

3.4 Suggested HPO terms (examples)

(ontology suggestions; not exhaustive) * Developmental delay — HP:0001263 * Intellectual disability — HP:0001249 * Hypotonia — HP:0001252 * Growth delay / failure to thrive — HP:0001508 / HP:0001507 * Microcephaly — HP:0000252 (reported in some cases) (sturiale2019alg12cdgnovelglycophenotype pages 6-8) * Recurrent respiratory infections — HP:0002205 (supported by recurrent pneumonias/infections) (grubenmann2002alg12mannosyltransferasedefect pages 2-3, ziburova2021anovelhomozygous pages 5-5) * Hypogammaglobulinemia — HP:0004313 (grubenmann2002alg12mannosyltransferasedefect pages 2-3, pascoal2024revisitingtheimmunopathology pages 4-6) * Abnormal coagulation / prolonged aPTT — HP:0011014 / HP:0030842 (sturiale2019alg12cdgnovelglycophenotype pages 6-8) * Cryptorchidism — HP:0000028; Micropenis — HP:0000054 (grubenmann2002alg12mannosyltransferasedefect pages 2-3)


4. Genetic / molecular information

4.1 Causal gene

  • ALG12 (approved symbol used in OpenTargets; Ensembl ENSG00000182858) (OpenTargets Search: ALG12-congenital disorder of glycosylation-ALG12)

4.2 Pathogenic variant classes (examples from primary reports)

Reported variants include missense, frameshift, and (in later literature) intronic splice‑altering variants; most evidence supports loss‑of‑function or hypomorphic loss‑of‑function. (grubenmann2002alg12mannosyltransferasedefect pages 1-2, tahata2019complexphenotypesin pages 1-2, ziburova2021anovelhomozygous pages 1-1)

Examples: * Compound heterozygous missense: T67M and R146Q in the first report. (grubenmann2002alg12mannosyltransferasedefect pages 1-2) * Frameshift: c.1001delA (p.N334TfsX15) reported in a family case series. (tahata2019complexphenotypesin pages 1-2) * Missense: c.1439T>C (p.Leu480Pro) reported as homozygous in a Slovak patient. (ziburova2021anovelhomozygous pages 1-1) * Compound heterozygous missense: c.367G>A (p.Gly123Arg) and c.1439T>C (p.Leu480Pro) in another patient. (sturiale2019alg12cdgnovelglycophenotype pages 6-8)

Population frequency note (limited): p.Leu480Pro was described as having a very low ExAC frequency (“8 × 10−6”). (sturiale2019alg12cdgnovelglycophenotype pages 6-8)

4.3 Modifier genes / epigenetics / chromosomal abnormalities

No ALG12‑CDG‑specific modifier genes, epigenetic mechanisms, or recurrent chromosomal abnormalities were identified in the retrieved texts.


5. Environmental information

ALG12‑CDG is a Mendelian disorder primarily driven by biallelic ALG12 variants; no consistent non‑genetic causal environmental exposures were identified.


6. Mechanism / pathophysiology

6.1 Causal chain (from gene defect to biochemical signature)

  1. ALG12 enzymatic defect in ER N‑glycan precursor assembly: ALG12 adds the 8th mannose residue during LLO synthesis. (ziburova2021anovelhomozygous pages 2-2, pascoal2024revisitingtheimmunopathology pages 4-6)
  2. Truncated LLO accumulation: patient fibroblasts showed accumulation of DolPP‑GlcNAc2Man7 and absence of mature DolPP‑GlcNAc2Man9Glc3. (grubenmann2002alg12mannosyltransferasedefect pages 2-3)
  3. Transfer of truncated glycans to proteins → hypoglycosylation: serum transferrin IEF shows a type‑I CDG pattern (decreased tetrasialotransferrin with increased disialo/asialo forms). (grubenmann2002alg12mannosyltransferasedefect pages 2-3)
  4. Systemic multisystem disease due to widespread hypoglycosylation affecting secreted and membrane proteins (neurodevelopment, immunity, coagulation, endocrine axes). (grubenmann2002alg12mannosyltransferasedefect pages 2-3, pascoal2024revisitingtheimmunopathology pages 4-6)

6.2 Biochemical abnormalities in serum glycomics

A 2021 report found MS evidence of impaired ALG12 activity: “analysis of neutral serum N‑glycans by mass spectrometry revealed the accumulation of GlcNAc2Man5–7 and decreased levels of GlcNAc2Man8–9.” (ziburova2021anovelhomozygous pages 1-1)

6.3 Immune involvement (expert synthesis)

A 2024 immunopathology review classifies ALG12‑CDG among “predominantly antibody deficiencies” characterized by “hypogammaglobulinemia and low IgG.” (Pascoal et al., 2024‑03; Frontiers in Immunology; DOI https://doi.org/10.3389/fimmu.2024.1350101) (pascoal2024revisitingtheimmunopathology pages 3-4, pascoal2024revisitingtheimmunopathology pages 4-6)

Mechanistic framing in the same review: defective glycosylation can lead to “defective antibody glycosylation,” reducing stability and Fc receptor binding, likely contributing to antibody deficiency and infection susceptibility. (pascoal2024revisitingtheimmunopathology pages 4-6)

6.4 Suggested GO / CL / pathway terms (examples)

  • GO Biological Process: protein N‑linked glycosylation; dolichol‑linked oligosaccharide biosynthetic process.
  • GO Cellular Component: endoplasmic reticulum membrane; ER lumen.
  • Cell Ontology (CL): B cell (CL:0000236), plasma cell (CL:0000786), hepatocyte (CL:0000182), neuron (CL:0000540) — reflecting immune, liver/coagulation, and neurodevelopmental involvement.
  • Reactome/KEGG pathway concept: N‑glycan precursor (LLO) biosynthesis in ER (ALG3/ALG9/ALG12 steps referenced in CDG literature). (pascoal2024revisitingtheimmunopathology pages 4-6)

7. Anatomical structures affected

Based on reported phenotypes and biochemical effects: * Central nervous system (neurodevelopmental delay, hypotonia) (UBERON:0000955 — brain) * Immune system (hypogammaglobulinemia, infections) (UBERON:0002405 — immune system) * Liver / plasma protein production (coagulation factors, antithrombin, transaminases) (UBERON:0002107 — liver) (grubenmann2002alg12mannosyltransferasedefect pages 2-3, ziburova2021anovelhomozygous pages 5-5) * Male reproductive system (micropenis, cryptorchidism) (UBERON:0000079 — male reproductive system) (grubenmann2002alg12mannosyltransferasedefect pages 2-3)

Subcellular localization relevant to mechanism: endoplasmic reticulum (GO:0005783) consistent with ER mannosyltransferase role and LLO assembly. (grubenmann2002alg12mannosyltransferasedefect pages 1-2, ziburova2021anovelhomozygous pages 2-2)


8. Temporal development

  • Typical onset: congenital/neonatal or infancy (newborn presentation and early‑life diagnosis described). (ziburova2021anovelhomozygous pages 1-1)
  • Course: variable; severe early fatal disease has been reported as well as longer survival with chronic multisystem impairment. (tahata2019complexphenotypesin pages 1-2, ziburova2021anovelhomozygous pages 5-5)

No formal staging system specific to ALG12‑CDG was identified.


9. Inheritance and population

9.1 Inheritance

Autosomal recessive, supported by homozygous and compound heterozygous cases. (ziburova2021anovelhomozygous pages 2-2, sturiale2019alg12cdgnovelglycophenotype pages 6-8)

9.2 Epidemiology

Robust incidence/prevalence estimates for ALG12‑CDG were not found in the retrieved evidence. However, multiple primary sources emphasize extreme rarity: * “To date, only 15 patients have been diagnosed with ALG12‑CDG globally.” (Ziburová et al., 2021‑09; DOI https://doi.org/10.1002/ajmg.a.62474) (ziburova2021anovelhomozygous pages 1-1) * Another excerpt states the Slovak case “brings the total number of published cases of this subtype to 16.” (ziburova2021anovelhomozygous pages 5-5)


10. Diagnostics

10.1 Clinical biochemical tests

Transferrin isoelectric focusing / carbohydrate‑deficient transferrin * Index case evidence: transferrin IEF demonstrated decreased tetrasialotransferrin with increased disialo/asialotransferrin (type‑I CDG pattern). (grubenmann2002alg12mannosyltransferasedefect pages 2-3)

Lipid‑linked oligosaccharide analysis (patient fibroblasts) * Demonstrated accumulation of truncated DolPP‑GlcNAc2Man7 and loss of mature DolPP‑GlcNAc2Man9Glc3. (grubenmann2002alg12mannosyltransferasedefect pages 2-3)

Serum N‑glycan profiling by MS * “Accumulation of GlcNAc2Man5–7 and decreased levels of GlcNAc2Man8–9” in neutral serum N‑glycans. (ziburova2021anovelhomozygous pages 1-1)

10.2 Genetic testing

Exome/genome sequencing is widely used for CDG diagnosis broadly. A 2023 review states: “CDG diagnosis has been at a rapid pace since the introduction of whole‑exome/whole‑genome sequencing as a diagnostic tool.” (Monticelli et al., 2023‑08; Orphanet J Rare Dis; DOI https://doi.org/10.1186/s13023-023-02852-w) (monticelli2023congenitaldisordersof pages 1-2)

The same review also emphasizes: “genetic analysis is the most reliable diagnostic.” (monticelli2023congenitaldisordersof pages 14-15)

10.3 Differential diagnosis

A comprehensive differential diagnosis list specific to ALG12‑CDG was not present in the retrieved texts. Practically, it overlaps with other CDG type I (LLO assembly/transfer) disorders, where transferrin IEF abnormalities prompt gene‑panel/WES/WGS confirmation.


11. Outcome / prognosis

Evidence indicates variable prognosis: * Severe early‑fatal multisystem disease is reported (e.g., infant death in a sibling group; fatal neonatal/infant outcomes in some cases). (tahata2019complexphenotypesin pages 1-2, ziburova2021anovelhomozygous pages 5-5) * Survivors can have chronic neurodevelopmental disability and recurrent infections. (tahata2019complexphenotypesin pages 1-2, grubenmann2002alg12mannosyltransferasedefect pages 2-3)

Quantitative survival curves or life expectancy estimates specific to ALG12‑CDG were not found in retrieved texts.


12. Treatment

12.1 Disease‑modifying therapy

No ALG12‑CDG‑specific disease‑modifying therapy was identified in the retrieved evidence.

12.2 Supportive and rehabilitative care (real‑world implementation)

  • Immunoglobulin replacement has been used in cases with hypogammaglobulinemia; the index case received “regular immunoglobulin infusions.” (grubenmann2002alg12mannosyltransferasedefect pages 2-3)
  • However, the 2024 immunopathology review notes that Ig infusion “attempts” in some ALG12‑CDG patients showed “apparent lack of success” (case‑dependent; limited published evidence). (pascoal2024revisitingtheimmunopathology pages 4-6)
  • Additional supportive management is implied by disease manifestations (infection management, nutritional support, coagulation monitoring).

Suggested MAXO terms (examples) * Immunoglobulin replacement therapy (MAXO term for IVIG/SCIG) * Anti‑infective therapy / infection prophylaxis * Nutritional support / enteral feeding * Coagulation factor replacement / management of coagulopathy * Physical therapy / occupational therapy / speech therapy for developmental impairment

12.3 Clinical trials

No interventional trials specific to ALG12‑CDG were found. A key real‑world research infrastructure is an observational CDG natural history study: * NCT04199000 (ClinicalTrials.gov; first posted 2019; recruiting): observational case‑only study with “genetically, enzymatically, or molecularly confirmed diagnosis of CDG or NGLY1 deficiency,” enrollment target 500; includes questionnaires, clinical exams, Nijmegen progression scale and PROMIS measures; allows biospecimen collection for biomarker/DNA studies. ALG12 is listed among keywords. URL: https://clinicaltrials.gov/study/NCT04199000 (NCT04199000 chunk 1, NCT04199000 chunk 2)


13. Prevention

No primary prevention exists for Mendelian ALG12‑CDG beyond reproductive and carrier testing strategies: * Carrier testing in at‑risk families (based on known familial variants) * Prenatal diagnosis / preimplantation genetic testing where appropriate

These approaches were not detailed in the retrieved corpus but follow standard practice for autosomal recessive disorders.


14. Other species / natural disease

No naturally occurring veterinary disease analogs for ALG12‑CDG were identified in the retrieved evidence.


15. Model organisms / experimental systems

Direct ALG12‑CDG functional modeling evidence in the retrieved corpus is limited but includes: * Yeast complementation/ortholog modeling: The original report used yeast functional complementation to show that ALG12 patient mutations fail to rescue an alg12 yeast mutant, supporting pathogenicity. (grubenmann2002alg12mannosyltransferasedefect pages 1-2)

No detailed mammalian or zebrafish ALG12‑specific in vivo phenotype data were retrieved in this tool run; therefore, this section should be revisited with dedicated model‑organism database searches (MGI/ZFIN/IMPC) if required.


Recent developments and latest research (prioritizing 2023–2024)

Diagnostic acceleration and unmet needs

A 2023 Orphanet Journal of Rare Diseases review (patent‑focused) summarizes the state of the field: * CDG comprises “more than 160” defects. (monticelli2023congenitaldisordersof pages 1-2) * “CDG diagnosis has been at a rapid pace since the introduction of whole‑exome/whole‑genome sequencing as a diagnostic tool.” (monticelli2023congenitaldisordersof pages 1-2) * Despite progress, “diagnostic tools, drugs, and biomarkers are still urgently needed.” (monticelli2023congenitaldisordersof pages 1-2)

Immune phenotype synthesis for ALG12‑CDG

A 2024 Frontiers in Immunology review places ALG12‑CDG into an inborn‑errors‑of‑immunity framing as predominantly antibody deficiency and discusses how glycosylation defects can compromise antibody properties and infection defense. (pascoal2024revisitingtheimmunopathology pages 3-4, pascoal2024revisitingtheimmunopathology pages 4-6)

Emerging translational methods

A 2024 targeted proteomics paper describes an MRM assay to quantify low‑abundance ER glycosyltransferases in CDG patient fibroblasts and explicitly situates ALG12 among the ER enzymes in the pathway context (“ALG3, ALG9, ALG12…”). This represents a real‑world implementation of quantitative proteomics for CDG mechanism and potentially diagnostics/biomarker development. (Lin et al., 2024‑01; Int J Mol Sci; DOI https://doi.org/10.3390/ijms25021191) (lin2024targetedproteomicsreveals pages 1-2)


Key statistics and data points (from retrieved studies)

  • Reported patient count: “only 15 patients” diagnosed globally by 2021; Slovak report increased published cases to 16. (ziburova2021anovelhomozygous pages 1-1, ziburova2021anovelhomozygous pages 5-5)
  • Characteristic serum N‑glycan shift: accumulation of GlcNAc2Man5–7 with decreased GlcNAc2Man8–9 (MS evidence). (ziburova2021anovelhomozygous pages 1-1)
  • Patent landscape (CDG, not ALG12‑specific): final list of 43 patents categorized into therapy (25), delivery (2), and diagnostics (17). (monticelli2023congenitaldisordersof pages 1-2)

Evidence organization artifact

The following table summarizes key facts for knowledge‑base ingestion:

Category Key findings (concise) Evidence type Primary citations (include PMID if available; otherwise DOI/URL)
Disease definition ALG12-congenital disorder of glycosylation (ALG12-CDG), historically CDG-Ig, is a rare type I CDG caused by deficiency of ALG12 ER α1,6-mannosyltransferase; severe multisystem disease with hypoglycosylation. OMIM/MIM reported in literature as #607143; one excerpt also cites OMIM:607144. Human case report, review Grubenmann et al., 2002, Hum Mol Genet, DOI: https://doi.org/10.1093/hmg/11.19.2331; Ziburová et al., 2021, AJMG A, DOI: https://doi.org/10.1002/ajmg.a.62474; Tahata et al., 2019, Mol Genet Metab, DOI: https://doi.org/10.1016/j.ymgme.2019.08.007 (grubenmann2002alg12mannosyltransferasedefect pages 1-2, ziburova2021anovelhomozygous pages 2-2, tahata2019complexphenotypesin pages 1-2)
Gene/enzyme function ALG12 encodes dolichol-P-mannose:Man7GlcNAc2-PP-dolichyl-α-6-mannosyltransferase / α-mannosyltransferase 8, which adds the 8th mannose to the lipid-linked oligosaccharide precursor during N-glycan biosynthesis in the ER. Human case report, review Grubenmann et al., 2002, DOI: https://doi.org/10.1093/hmg/11.19.2331; Ziburová et al., 2021, DOI: https://doi.org/10.1002/ajmg.a.62474; Pascoal et al., 2024, Front Immunol, DOI: https://doi.org/10.3389/fimmu.2024.1350101 (grubenmann2002alg12mannosyltransferasedefect pages 1-2, ziburova2021anovelhomozygous pages 2-2, pascoal2024revisitingtheimmunopathology pages 4-6)
Inheritance Autosomal recessive; reported in homozygous and compound heterozygous states. Human case report/series Ziburová et al., 2021, DOI: https://doi.org/10.1002/ajmg.a.62474; Tahata et al., 2019, DOI: https://doi.org/10.1016/j.ymgme.2019.08.007; Sturiale et al., 2019, DOI: https://doi.org/10.1007/s10719-019-09890-2 (ziburova2021anovelhomozygous pages 2-2, tahata2019complexphenotypesin pages 1-2, sturiale2019alg12cdgnovelglycophenotype pages 6-8)
Core phenotypes Commonly reported: psychomotor/intellectual delay, hypotonia, growth retardation/failure to thrive, microcephaly, dysmorphic facial features, recurrent infections, feeding difficulties, respiratory distress, skeletal abnormalities; variable severity including neonatal/infantile fatal cases and milder presentations. Human case report/series, review Grubenmann et al., 2002, DOI: https://doi.org/10.1093/hmg/11.19.2331; Tahata et al., 2019, DOI: https://doi.org/10.1016/j.ymgme.2019.08.007; Ziburová et al., 2021, DOI: https://doi.org/10.1002/ajmg.a.62474; de la Morena-Barrio et al., 2020, DOI: https://doi.org/10.1002/mgg3.1304 (grubenmann2002alg12mannosyltransferasedefect pages 1-2, tahata2019complexphenotypesin pages 1-2, ziburova2021anovelhomozygous pages 5-5)
Immune involvement Predominantly antibody deficiency phenotype: hypogammaglobulinemia/low IgG, recurrent severe bacterial or sinopulmonary infections, altered lymphocyte counts/dysfunction. 2024 review groups ALG12-CDG with predominantly antibody deficiencies. Human case report, review Grubenmann et al., 2002, DOI: https://doi.org/10.1093/hmg/11.19.2331; Pascoal et al., 2024, DOI: https://doi.org/10.3389/fimmu.2024.1350101; Ziburová et al., 2021, DOI: https://doi.org/10.1002/ajmg.a.62474 (grubenmann2002alg12mannosyltransferasedefect pages 2-3, pascoal2024revisitingtheimmunopathology pages 4-6, ziburova2021anovelhomozygous pages 6-7)
Coagulation/endocrine Reported coagulation abnormalities include low antithrombin III, prolonged APTT, decreased coagulation factors; endocrine/metabolic findings include undetectable IGF-1/IGF-BP3, hypoglycemia, low cholesterol, elevated transaminases, and male genital anomalies (micropenis/cryptorchidism). Human case report/series Grubenmann et al., 2002, DOI: https://doi.org/10.1093/hmg/11.19.2331; Sturiale et al., 2019, DOI: https://doi.org/10.1007/s10719-019-09890-2; Ziburová et al., 2021, DOI: https://doi.org/10.1002/ajmg.a.62474; Tahata et al., 2019, DOI: https://doi.org/10.1016/j.ymgme.2019.08.007 (grubenmann2002alg12mannosyltransferasedefect pages 2-3, sturiale2019alg12cdgnovelglycophenotype pages 6-8, ziburova2021anovelhomozygous pages 5-5, tahata2019complexphenotypesin pages 1-2)
Diagnostics (transferrin IEF, LLO, serum N-glycan MS) Serum transferrin IEF shows a type I CDG pattern with decreased tetrasialotransferrin and increased disialo-/asialotransferrin. Patient fibroblasts can show truncated LLO with accumulation of DolPP-GlcNAc2Man7 and absence/reduction of mature DolPP-GlcNAc2Man9Glc3. Serum N-glycan MS/MALDI-TOF shows accumulation of GlcNAc2Man5-7 with decreased GlcNAc2Man8-9; transferrin/total serum glycomics can reveal mono-glycosylated transferrin and increased high-mannose/hybrid glycans. Human case report/series Grubenmann et al., 2002, DOI: https://doi.org/10.1093/hmg/11.19.2331; Ziburová et al., 2021, DOI: https://doi.org/10.1002/ajmg.a.62474; Sturiale et al., 2019, DOI: https://doi.org/10.1007/s10719-019-09890-2 (grubenmann2002alg12mannosyltransferasedefect pages 2-3, ziburova2021anovelhomozygous pages 1-1, sturiale2019alg12cdgnovelglycophenotype pages 6-8)
Variant examples Reported pathogenic/likely pathogenic examples include compound heterozygous p.T67M and p.R146Q; c.1001delA (p.N334Tfs*15) with c.671C>T (p.T224M, reported as VUS in one series); c.367G>A (p.Gly123Arg) and c.1439T>C (p.Leu480Pro); homozygous c.1439T>C (p.Leu480Pro); novel p.Val26Asp. 2025 report (outside requested priority window) adds an intronic splice variant upstream of exon 2. Human case report/series Grubenmann et al., 2002, DOI: https://doi.org/10.1093/hmg/11.19.2331; Tahata et al., 2019, DOI: https://doi.org/10.1016/j.ymgme.2019.08.007; Sturiale et al., 2019, DOI: https://doi.org/10.1007/s10719-019-09890-2; Ziburová et al., 2021, DOI: https://doi.org/10.1002/ajmg.a.62474 (grubenmann2002alg12mannosyltransferasedefect pages 1-2, tahata2019complexphenotypesin pages 1-2, sturiale2019alg12cdgnovelglycophenotype pages 6-8, ziburova2021anovelhomozygous pages 1-1)
Epidemiology/patient counts Extremely rare. Literature excerpts report “only 15 patients” worldwide by 2021 and “16 published cases” after the Slovak case; no robust prevalence/incidence estimate identified in available context. Human case report, review Ziburová et al., 2021, DOI: https://doi.org/10.1002/ajmg.a.62474; Piedade et al., 2022, J Rare Dis, DOI: https://doi.org/10.1007/s44162-022-00003-6 (ziburova2021anovelhomozygous pages 1-1, ziburova2021anovelhomozygous pages 5-5)
Management/supportive care No disease-specific curative therapy identified in available context. Supportive care includes immunoglobulin replacement/IVIG in patients with hypogammaglobulinemia, management of infections, nutritional/supportive multidisciplinary care, and monitoring of coagulation/endocrine issues. Reported response to Ig infusion may be variable or limited in some ALG12-CDG cases. Human case report, review Grubenmann et al., 2002, DOI: https://doi.org/10.1093/hmg/11.19.2331; Pascoal et al., 2024, DOI: https://doi.org/10.3389/fimmu.2024.1350101 (grubenmann2002alg12mannosyltransferasedefect pages 2-3, pascoal2024revisitingtheimmunopathology pages 4-6)
Research/real-world implementations Real-world diagnosis increasingly uses WES/WGS for CDG discovery/confirmation; MS-based glycomics (MALDI-MS/UHPLC-ESI-MS) refines subtype-specific glycophenotypes; targeted proteomics/MRM assays for ER glycosyltransferases were introduced in 2024 and include the ALG12 pathway, supporting translational diagnostics/research. Patent review notes CDG diagnosis accelerated with WES/WGS and that diagnostic tools, drugs, and biomarkers remain urgently needed. Review, translational research Monticelli et al., 2023, DOI: https://doi.org/10.1186/s13023-023-02852-w; Sturiale et al., 2019, DOI: https://doi.org/10.1007/s10719-019-09890-2; Lin et al., 2024, DOI: https://doi.org/10.3390/ijms25021191 (monticelli2023congenitaldisordersof pages 1-2, sturiale2019alg12cdgnovelglycophenotype pages 1-2, lin2024targetedproteomicsreveals pages 1-2)
Clinical trial registry (NCT04199000) Ongoing observational natural-history study: “Clinical and Basic Investigations Into Congenital Disorders of Glycosylation” (NCT04199000), recruiting, case-only, target enrollment 500, includes genetically/enzymatically/molecularly confirmed CDG or NGLY1 deficiency; outcomes include disease severity/progression measures and biomarker collection. ALG12 is listed among keywords, making the registry relevant to ALG12-CDG. Trial registry ClinicalTrials.gov NCT04199000: https://clinicaltrials.gov/study/NCT04199000 (NCT04199000 chunk 1, NCT04199000 chunk 2)

Table: This table summarizes key disease facts, clinical and molecular findings, diagnostics, management, and current research implementations for ALG12-CDG using only evidence available in the conversation context. It is designed for rapid knowledge-base ingestion with source-linked citations.


Limitations of this report (from available tool‑retrieved corpus)

  1. PMIDs were not available in the retrieved text snippets for several primary papers (DOIs/URLs were available); therefore, PMID‑level citation could not be consistently provided.
  2. Orphanet/ICD/MeSH identifiers were not present in the retrieved full texts; normalization would require direct database queries.
  3. Quantitative phenotype frequencies (percentages across cohorts) are largely unavailable because ALG12‑CDG literature is dominated by small case reports/series.
  4. Animal model phenotype data specific to ALG12 were not retrieved beyond yeast complementation.

Source URLs and publication dates (high‑priority items)

  • Grubenmann et al., “ALG12 mannosyltransferase defect in congenital disorder of glycosylation type Ig.” Human Molecular Genetics. 2002‑09. https://doi.org/10.1093/hmg/11.19.2331 (grubenmann2002alg12mannosyltransferasedefect pages 1-2)
  • Ziburová et al., “A novel homozygous mutation in the human ALG12 gene…” American Journal of Medical Genetics A. 2021‑09. https://doi.org/10.1002/ajmg.a.62474 (ziburova2021anovelhomozygous pages 1-1)
  • Monticelli et al., “Congenital disorders of glycosylation: narration of a story through its patents.” Orphanet J Rare Dis. 2023‑08. https://doi.org/10.1186/s13023-023-02852-w (monticelli2023congenitaldisordersof pages 1-2)
  • Pascoal et al., “Revisiting the immunopathology of congenital disorders of glycosylation: an updated review.” Frontiers in Immunology. 2024‑03. https://doi.org/10.3389/fimmu.2024.1350101 (pascoal2024revisitingtheimmunopathology pages 1-2)
  • Lin et al., “Targeted Proteomics Reveals Quantitative Differences…” Int J Mol Sci. 2024‑01. https://doi.org/10.3390/ijms25021191 (lin2024targetedproteomicsreveals pages 1-2)
  • ClinicalTrials.gov natural history study: NCT04199000. First posted 2019; recruiting. https://clinicaltrials.gov/study/NCT04199000 (NCT04199000 chunk 1)

References

  1. (grubenmann2002alg12mannosyltransferasedefect pages 1-2): C. Grubenmann, C. Frank, S. Kjaergaard, E. Berger, M. Aebi, and T. Hennet. Alg12 mannosyltransferase defect in congenital disorder of glycosylation type lg. Human molecular genetics, 11 19:2331-9, Sep 2002. URL: https://doi.org/10.1093/hmg/11.19.2331, doi:10.1093/hmg/11.19.2331. This article has 103 citations and is from a domain leading peer-reviewed journal.

  2. (grubenmann2002alg12mannosyltransferasedefect pages 2-3): C. Grubenmann, C. Frank, S. Kjaergaard, E. Berger, M. Aebi, and T. Hennet. Alg12 mannosyltransferase defect in congenital disorder of glycosylation type lg. Human molecular genetics, 11 19:2331-9, Sep 2002. URL: https://doi.org/10.1093/hmg/11.19.2331, doi:10.1093/hmg/11.19.2331. This article has 103 citations and is from a domain leading peer-reviewed journal.

  3. (ziburova2021anovelhomozygous pages 1-1): Jana Ziburová, Marek Nemčovič, Sergej Šesták, Jana Bellová, Zuzana Pakanová, Barbara Siváková, Anna Šalingová, Claudia Šebová, Mária Ostrožlíková, Dimitra‐Evanthia Lekka, Jana Brucknerová, Ingrid Brucknerová, Martina Skokňová, Alexandra Mc Cullough, Gabriela Hrčková, Anna Hlavatá, Vladimír Bzdúch, Ján Mucha, and Peter Baráth. A novel homozygous mutation in the human alg12 gene results in an aberrant profile of oligomannose n‐glycans in patient's serum. American Journal of Medical Genetics. Part a, 185:3494-3501, Sep 2021. URL: https://doi.org/10.1002/ajmg.a.62474, doi:10.1002/ajmg.a.62474. This article has 17 citations and is from a peer-reviewed journal.

  4. (monticelli2023congenitaldisordersof pages 1-2): Maria Monticelli, Tania D’Onofrio, Jaak Jaeken, Eva Morava, Giuseppina Andreotti, and Maria Vittoria Cubellis. Congenital disorders of glycosylation: narration of a story through its patents. Orphanet Journal of Rare Diseases, Aug 2023. URL: https://doi.org/10.1186/s13023-023-02852-w, doi:10.1186/s13023-023-02852-w. This article has 19 citations and is from a peer-reviewed journal.

  5. (pascoal2024revisitingtheimmunopathology pages 4-6): Carlota Pascoal, Rita Francisco, Patrícia Mexia, Beatriz Luís Pereira, Pedro Granjo, Helena Coelho, Mariana Barbosa, Vanessa dos Reis Ferreira, and Paula Alexandra Videira. Revisiting the immunopathology of congenital disorders of glycosylation: an updated review. Frontiers in Immunology, Mar 2024. URL: https://doi.org/10.3389/fimmu.2024.1350101, doi:10.3389/fimmu.2024.1350101. This article has 15 citations and is from a peer-reviewed journal.

  6. (OpenTargets Search: ALG12-congenital disorder of glycosylation-ALG12): Open Targets Query (ALG12-congenital disorder of glycosylation-ALG12, 2 results). Buniello, A. et al. (2025). Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery. Nucleic Acids Research.

  7. (ziburova2021anovelhomozygous pages 2-2): Jana Ziburová, Marek Nemčovič, Sergej Šesták, Jana Bellová, Zuzana Pakanová, Barbara Siváková, Anna Šalingová, Claudia Šebová, Mária Ostrožlíková, Dimitra‐Evanthia Lekka, Jana Brucknerová, Ingrid Brucknerová, Martina Skokňová, Alexandra Mc Cullough, Gabriela Hrčková, Anna Hlavatá, Vladimír Bzdúch, Ján Mucha, and Peter Baráth. A novel homozygous mutation in the human alg12 gene results in an aberrant profile of oligomannose n‐glycans in patient's serum. American Journal of Medical Genetics. Part a, 185:3494-3501, Sep 2021. URL: https://doi.org/10.1002/ajmg.a.62474, doi:10.1002/ajmg.a.62474. This article has 17 citations and is from a peer-reviewed journal.

  8. (sturiale2019alg12cdgnovelglycophenotype pages 1-2): Luisa Sturiale, Sebastiano Bianca, Domenico Garozzo, Alessandra Terracciano, Emanuele Agolini, Angela Messina, Angelo Palmigiano, Francesca Esposito, Chiara Barone, Antonio Novelli, Agata Fiumara, Jaak Jaeken, and Rita Barone. Alg12-cdg: novel glycophenotype insights endorse the molecular defect. Glycoconjugate Journal, 36:461-472, Sep 2019. URL: https://doi.org/10.1007/s10719-019-09890-2, doi:10.1007/s10719-019-09890-2. This article has 22 citations and is from a peer-reviewed journal.

  9. (tahata2019complexphenotypesin pages 1-2): Shawn Tahata, Lauren B. Gunderson, Brendan Lanpher, and E. Morava. Complex phenotypes in alg12-congenital disorder of glycosylation (alg12-cdg): case series and review of the literature. Molecular genetics and metabolism, 128:409-414, Dec 2019. URL: https://doi.org/10.1016/j.ymgme.2019.08.007, doi:10.1016/j.ymgme.2019.08.007. This article has 25 citations and is from a peer-reviewed journal.

  10. (ziburova2021anovelhomozygous pages 7-8): Jana Ziburová, Marek Nemčovič, Sergej Šesták, Jana Bellová, Zuzana Pakanová, Barbara Siváková, Anna Šalingová, Claudia Šebová, Mária Ostrožlíková, Dimitra‐Evanthia Lekka, Jana Brucknerová, Ingrid Brucknerová, Martina Skokňová, Alexandra Mc Cullough, Gabriela Hrčková, Anna Hlavatá, Vladimír Bzdúch, Ján Mucha, and Peter Baráth. A novel homozygous mutation in the human alg12 gene results in an aberrant profile of oligomannose n‐glycans in patient's serum. American Journal of Medical Genetics. Part a, 185:3494-3501, Sep 2021. URL: https://doi.org/10.1002/ajmg.a.62474, doi:10.1002/ajmg.a.62474. This article has 17 citations and is from a peer-reviewed journal.

  11. (pascoal2024revisitingtheimmunopathology pages 1-2): Carlota Pascoal, Rita Francisco, Patrícia Mexia, Beatriz Luís Pereira, Pedro Granjo, Helena Coelho, Mariana Barbosa, Vanessa dos Reis Ferreira, and Paula Alexandra Videira. Revisiting the immunopathology of congenital disorders of glycosylation: an updated review. Frontiers in Immunology, Mar 2024. URL: https://doi.org/10.3389/fimmu.2024.1350101, doi:10.3389/fimmu.2024.1350101. This article has 15 citations and is from a peer-reviewed journal.

  12. (ziburova2021anovelhomozygous pages 5-5): Jana Ziburová, Marek Nemčovič, Sergej Šesták, Jana Bellová, Zuzana Pakanová, Barbara Siváková, Anna Šalingová, Claudia Šebová, Mária Ostrožlíková, Dimitra‐Evanthia Lekka, Jana Brucknerová, Ingrid Brucknerová, Martina Skokňová, Alexandra Mc Cullough, Gabriela Hrčková, Anna Hlavatá, Vladimír Bzdúch, Ján Mucha, and Peter Baráth. A novel homozygous mutation in the human alg12 gene results in an aberrant profile of oligomannose n‐glycans in patient's serum. American Journal of Medical Genetics. Part a, 185:3494-3501, Sep 2021. URL: https://doi.org/10.1002/ajmg.a.62474, doi:10.1002/ajmg.a.62474. This article has 17 citations and is from a peer-reviewed journal.

  13. (sturiale2019alg12cdgnovelglycophenotype pages 6-8): Luisa Sturiale, Sebastiano Bianca, Domenico Garozzo, Alessandra Terracciano, Emanuele Agolini, Angela Messina, Angelo Palmigiano, Francesca Esposito, Chiara Barone, Antonio Novelli, Agata Fiumara, Jaak Jaeken, and Rita Barone. Alg12-cdg: novel glycophenotype insights endorse the molecular defect. Glycoconjugate Journal, 36:461-472, Sep 2019. URL: https://doi.org/10.1007/s10719-019-09890-2, doi:10.1007/s10719-019-09890-2. This article has 22 citations and is from a peer-reviewed journal.

  14. (pascoal2024revisitingtheimmunopathology pages 3-4): Carlota Pascoal, Rita Francisco, Patrícia Mexia, Beatriz Luís Pereira, Pedro Granjo, Helena Coelho, Mariana Barbosa, Vanessa dos Reis Ferreira, and Paula Alexandra Videira. Revisiting the immunopathology of congenital disorders of glycosylation: an updated review. Frontiers in Immunology, Mar 2024. URL: https://doi.org/10.3389/fimmu.2024.1350101, doi:10.3389/fimmu.2024.1350101. This article has 15 citations and is from a peer-reviewed journal.

  15. (monticelli2023congenitaldisordersof pages 14-15): Maria Monticelli, Tania D’Onofrio, Jaak Jaeken, Eva Morava, Giuseppina Andreotti, and Maria Vittoria Cubellis. Congenital disorders of glycosylation: narration of a story through its patents. Orphanet Journal of Rare Diseases, Aug 2023. URL: https://doi.org/10.1186/s13023-023-02852-w, doi:10.1186/s13023-023-02852-w. This article has 19 citations and is from a peer-reviewed journal.

  16. (NCT04199000 chunk 1): Eva Morava-Kozicz. Clinical and Basic Investigations Into Congenital Disorders of Glycosylation. Icahn School of Medicine at Mount Sinai. 2019. ClinicalTrials.gov Identifier: NCT04199000

  17. (NCT04199000 chunk 2): Eva Morava-Kozicz. Clinical and Basic Investigations Into Congenital Disorders of Glycosylation. Icahn School of Medicine at Mount Sinai. 2019. ClinicalTrials.gov Identifier: NCT04199000

  18. (lin2024targetedproteomicsreveals pages 1-2): Qingsong Lin, Lei Zhou, Chuen Lam, Roman Sakson, Lars Beedgen, Patrick Bernhard, K. M. Alp, Nicole Lübbehusen, R. Röth, Beate Niesler, Marcin Luzarowski, Olga Shevchuk, Matthias P. Mayer, Christian Thiel, and Thomas Ruppert. Targeted proteomics reveals quantitative differences in low-abundance glycosyltransferases of patients with congenital disorders of glycosylation. International Journal of Molecular Sciences, 25:1191, Jan 2024. URL: https://doi.org/10.3390/ijms25021191, doi:10.3390/ijms25021191. This article has 5 citations.

  19. (ziburova2021anovelhomozygous pages 6-7): Jana Ziburová, Marek Nemčovič, Sergej Šesták, Jana Bellová, Zuzana Pakanová, Barbara Siváková, Anna Šalingová, Claudia Šebová, Mária Ostrožlíková, Dimitra‐Evanthia Lekka, Jana Brucknerová, Ingrid Brucknerová, Martina Skokňová, Alexandra Mc Cullough, Gabriela Hrčková, Anna Hlavatá, Vladimír Bzdúch, Ján Mucha, and Peter Baráth. A novel homozygous mutation in the human alg12 gene results in an aberrant profile of oligomannose n‐glycans in patient's serum. American Journal of Medical Genetics. Part a, 185:3494-3501, Sep 2021. URL: https://doi.org/10.1002/ajmg.a.62474, doi:10.1002/ajmg.a.62474. This article has 17 citations and is from a peer-reviewed journal.