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1
Definitions
2
Inheritance
8
Pathophys.
28
Phenotypes
31
Pathograph
2
Genes
8
Medical Actions
2
Subtypes
1
Datasets
1
Trials
1
Deep Research
🏷

Classifications

Harrison's Chapter
GENETICS_ENVIRONMENT_DISEASE NEUROLOGIC
📘

Definitions

1
International consensus clinical definition
Kabuki syndrome is clinically defined by infantile hypotonia with developmental delay or intellectual disability together with either a pathogenic KMT2D or KDM6A variant or the characteristic evolving Kabuki facial gestalt.
CASE_DEFINITION Molecularly confirmed and clinically recognizable Kabuki syndrome
Show evidence (1 reference)
PMID:30514738 SUPPORT Human Clinical
"The authors propose that a definitive diagnosis can be made in an individual of any age with a history of infantile hypotonia, developmental delay and/or intellectual disability, and one or both of the following major criteria: (1) a pathogenic or likely pathogenic variant in KMT2D or KDM6A; and..."
Supports a modern consensus case definition for Kabuki syndrome.
👪

Inheritance

2
Autosomal dominant inheritance HP:0000006
Most Kabuki syndrome cases are caused by heterozygous KMT2D variants and behave as an autosomal dominant disorder, usually arising de novo.
Autosomal dominant inheritance
Show evidence (2 references)
PMID:20711175 SUPPORT Human Clinical
"We demonstrate the successful application of exome sequencing to discover a gene for an autosomal dominant disorder, Kabuki syndrome (OMIM%147920)."
Supports autosomal dominant inheritance for the common KMT2D-related form.
ORPHA:2322 SUPPORT
"Autosomal dominant"
Orphanet classifies Kabuki syndrome inheritance as autosomal dominant.
X-linked dominant inheritance HP:0001423
A minority of cases are caused by heterozygous pathogenic variants or deletions in the X-linked gene KDM6A.
X-linked dominant inheritance
Show evidence (1 reference)
PMID:22197486 SUPPORT Human Clinical
"Here, we describe three KS individuals with de novo partial or complete deletions of an X chromosome gene, KDM6A, that encodes a histone demethylase that interacts with MLL2."
Supports an X-linked disease mechanism for the KDM6A-related form.

Subtypes

2
KMT2D-related Kabuki syndrome MONDO:0007843
KMT2D hgnc:7133
Autosomal dominant Kabuki syndrome caused by pathogenic KMT2D variants and representing the major molecular subtype.
Show evidence (2 references)
PMID:20711175 SUPPORT Human Clinical
"Our results strongly suggest that mutations in MLL2 are a major cause of Kabuki syndrome."
Supports KMT2D as the principal disease gene and major subtype.
ORPHA:2322 SUPPORT
"KMT2D | lysine methyltransferase 2D | hgnc:7133 | Disease-causing germline mutation(s) in"
Orphanet lists KMT2D as a disease-causing gene for Kabuki syndrome.
KDM6A-related Kabuki syndrome MONDO:0010465
KDM6A hgnc:12637
Less common Kabuki syndrome subtype caused by pathogenic KDM6A variants.
Show evidence (2 references)
PMID:22197486 SUPPORT Human Clinical
"This study identifies KDM6A mutations as another cause of KS and highlights the growing role of histone methylases and histone demethylases in multiple-congenital-anomaly and intellectual-disability syndromes."
Supports a distinct KDM6A-related molecular subtype.
ORPHA:2322 SUPPORT
"KDM6A | lysine demethylase 6A | hgnc:12637 | Disease-causing germline mutation(s) in"
Orphanet lists KDM6A as a disease-causing gene for Kabuki syndrome.

Pathophysiology

8
Chromatin dysregulation from KMT2D and KDM6A haploinsufficiency
Kabuki syndrome is a disorder of epigenetic regulation in which loss of KMT2D or KDM6A function perturbs COMPASS-associated chromatin control. Truncating KMT2D variants frequently undergo nonsense-mediated decay, reducing KMT2D dosage and dysregulating developmental target genes. KDM6A lesions disrupt a cooperating histone demethylase in the same chromatin-regulatory pathway.
neural crest cell CL:0011012 B cell CL:0000236
chromatin organization GO:0006325 regulation of gene expression GO:0010468
Show evidence (6 references)
PMID:24633898 SUPPORT Human Clinical
"Kabuki syndrome (KS) is a multiple congenital anomalies syndrome characterized by characteristic facial features and varying degrees of mental retardation, caused by mutations in KMT2D/MLL2 and KDM6A/UTX genes."
Establishes the two principal disease genes and the syndrome's developmental basis.
PMID:24633898 SUPPORT In Vitro
"We found that a number of KMT2D truncating mutations result in mRNA degradation through the nonsense-mediated mRNA decay, contributing to protein haploinsufficiency."
Supports haploinsufficiency as a core molecular mechanism.
PMID:22197486 SUPPORT Human Clinical
"Here, we describe three KS individuals with de novo partial or complete deletions of an X chromosome gene, KDM6A, that encodes a histone demethylase that interacts with MLL2."
Connects KDM6A loss to the same chromatin-regulatory disease mechanism.
+ 3 more references
Neural crest developmental dysregulation
Experimental models indicate that Kabuki syndrome is a neurocristopathy. Impaired chromatin regulation in neural crest derivatives disrupts craniofacial morphogenesis, left-sided cardiovascular development, and postnatal growth, helping explain the characteristic facial gestalt, congenital heart disease, and growth deficiency seen clinically.
neural crest cell CL:0011012 chondrocyte CL:0000138
neural crest cell migration GO:0001755 neural crest cell migration involved in heart formation GO:0003147
Show evidence (7 references)
PMID:29073101 SUPPORT Model Organism
"We now establish Kabuki syndrome as a neurocristopathy, whereby the majority of clinical features are modeled in mice carrying neural crest (NC) deletion of UTX, including craniofacial dysmorphism, cardiac defects, and postnatal growth retardation."
Supports neural crest dysfunction as a unifying developmental mechanism.
PMID:31479440 SUPPORT Model Organism
"Our zebrafish Kabuki Syndrome model reveals a regulatory link between the Notch pathway and Kmt2d during endothelium and endocardium patterning and shows that pharmacological inhibition of Notch signaling rebalances Rbpj protein levels and rescues the cardiovascular phenotype by enhancing..."
Supports a specific cardiovascular mechanism downstream of KMT2D deficiency.
PMID:32541010 SUPPORT Model Organism
"KMT2D NCC knockout mice demonstrate hypoplasia with reductions in frontonasal bone lengths."
Establishes that conditional KMT2D loss in neural crest is sufficient to produce the craniofacial phenotype.
+ 4 more references
B-cell terminal differentiation defect
KMT2D deficiency impairs terminal B-cell maturation, class-switched memory B-cell formation, and humoral immune competence. This creates a clinically important immune phenotype with hypogammaglobulinemia, IgA deficiency, recurrent infections, and in a subset of patients autoimmune disease.
B cell CL:0000236
B cell differentiation GO:0030183 somatic hypermutation of immunoglobulin genes GO:0016446
Show evidence (6 references)
PMID:26194542 SUPPORT Human Clinical
"Among the patients with KMT2D mutations (KMT2D(Mut/+)), hypogammaglobulinemia was detected in all but 1 patient, with IgA deficiency affecting 90% of patients and a deficiency in at least 1 other isoform seen in 40% of patients."
Supports a high-burden humoral immunodeficiency phenotype in KMT2D-related disease.
PMID:26194542 SUPPORT In Vitro
"Impaired terminal differentiation was noted in primary B cells from patients with KMT2D(Mut/+) mutations."
Supports a cell-intrinsic B-cell maturation defect.
PMID:31363182 SUPPORT Human Clinical
"Overall, 44.1% (78/177) and 58.2% (46/79) of KS individuals exhibited infection susceptibility and hypogammaglobulinemia, respectively;"
Confirms that immune dysfunction and autoimmunity are common in a large registry cohort.
+ 3 more references
Postnatal neurogenesis deficit
Animal data support a chromatin-based neurodevelopmental mechanism in which reduced KMT2D activity decreases dentate gyrus H3K4me3, impairs postnatal neurogenesis, and contributes to hippocampal-dependent memory deficits.
neuron CL:0000540
neuron development GO:0048666
Show evidence (3 references)
PMID:25273096 SUPPORT Model Organism
"In vivo, deficiency of H3K4me3 in the dentate gyrus granule cell layer of Kmt2d(+/βGeo) mice correlated with reduced neurogenesis and hippocampal memory defects."
Supports a mechanistic link between KMT2D-dependent chromatin defects and cognitive impairment.
PMID:33738331 SUPPORT Model Organism
"orally administered TAK-418 increases the numbers of newly born doublecortin (DCX)+ cells and processes in the hippocampus in a dose-dependent manner."
Confirms postnatal neurogenesis deficit can be reversed by restoring H3K4 methylation balance via KDM1A inhibition.
PMID:38702196 SUPPORT Model Organism
"Neurons, but not B or T cells, show preferential chromatin disruption at CpG islands and at regulatory elements linked to aging."
Demonstrates that neuronal chromatin disruption in Kabuki syndrome is cell-type specific and distinct from blood cell episignatures.
Cardiomyocyte gene-expression dysregulation
Beyond a neural-crest contribution, KMT2D acts cell-autonomously in cardiac precursors and cardiomyocytes during cardiogenesis. Myocardial Kmt2d deletion in mice reduces enhancer/promoter H3K4me1 and H3K4me2, downregulates ion-transport and cell-cycle genes, and produces altered calcium handling. Zebrafish models additionally implicate Notch dysregulation during endocardial patterning, contributing to congenital heart disease.
cardiac muscle cell CL:0000746
heart development GO:0007507 Notch signaling pathway GO:0007219 outflow tract morphogenesis GO:0003151
Show evidence (4 references)
PMID:26932671 SUPPORT Model Organism
"we demonstrate a requirement for KMT2D in cardiac precursors and cardiomyocytes during cardiogenesis in mice."
Establishes cell-autonomous KMT2D requirement in cardiomyocytes during heart development.
PMID:26932671 SUPPORT Model Organism
"myocardial Kmt2d deletion led to decreased H3K4me1 and H3K4me2 at enhancers and promoters."
Demonstrates the chromatin-level molecular consequence of KMT2D loss in cardiomyocytes.
PMID:31479440 SUPPORT Model Organism
"Our zebrafish Kabuki Syndrome model reveals a regulatory link between the Notch pathway and Kmt2d during endothelium and endocardium patterning"
Implicates Notch dysregulation as a downstream cardiovascular mechanism in Kabuki syndrome.
+ 1 more reference
Chondrocyte differentiation defect
KMT2D is required for hypertrophic chondrocyte differentiation and endochondral ossification. KS1 and KS2 cellular models exhibit precocious chondrocyte differentiation, shortened growth-plate hypertrophic zones, and convergent transcriptomic dysregulation downstream of SOX9 and RUNX2 enhancer programs. This contributes to postnatal growth deficiency, skeletal anomalies, and cleft palate via cranial-base ossification defects.
chondrocyte CL:0000138 hypertrophic chondrocyte CL:0000138
chondrocyte differentiation GO:0002062 endochondral ossification GO:0001958
Show evidence (4 references)
PMID:38857303 SUPPORT Model Organism
"Kdm6atm1d/+ growth plates are also shorter, due to decreases in hypertrophic chondrocyte size and hypertrophic zone height."
Demonstrates a growth-plate hypertrophic chondrocyte defect underlying KS2 short stature.
PMID:38857303 SUPPORT In Vitro
"RNA-seq simultaneously on Kmt2d-/-, Kdm6a-/-, and control lines at Days 7 and 14 of differentiation. This revealed surprising resemblance in gene expression between Kmt2d-/- and Kdm6a-/-"
Supports a convergent KS1/KS2 chondrocyte transcriptomic program.
PMID:38857303 SUPPORT In Vitro
"we found that Kdm6a-/- cells undergo premature, enhanced differentiation towards chondrocytes compared to Kdm6a+/+ controls."
Direct in vitro evidence that KDM6A loss drives premature, enhanced chondrocyte differentiation, mirroring the precocious differentiation seen in KS1 Kmt2d-/- chondrocytes and underlying the growth-plate defect.
+ 1 more reference
Pancreatic beta-cell dysfunction
Pathogenic variants in KMT2D and KDM6A predispose to congenital hyperinsulinism through dysregulated insulin secretion in pancreatic beta cells. Hypoglycemia frequently presents in the neonatal period and is typically diazoxide-responsive.
pancreatic beta cell CL:0000169
insulin secretion GO:0030073 regulation of insulin secretion GO:0050796
Show evidence (2 references)
PMID:29907798 SUPPORT Human Clinical
"Molecular diagnoses of KS were established by identification of pathogenic variants in KMT2D (n = 5) and KDM6A (n = 4)."
Confirms that both KMT2D and KDM6A loss can present with hyperinsulinism, supporting a shared beta-cell mechanism.
PMID:38859884 SUPPORT Human Clinical
"Diazoxide trial was conducted in 25 children, 92% of whom were responsive."
Demonstrates that hyperinsulinism in KS is typically diazoxide-responsive, consistent with a regulatory rather than structural beta-cell defect.
Cochlear outer hair cell dysfunction
KMT2D loss causes cell-intrinsic cochlear outer hair cell dysfunction, contributing to sensorineural hearing loss in addition to the conductive component from recurrent otitis media. Mouse models show diminished distortion product otoacoustic emissions despite no gross cochlear structural malformation on micro-CT.
outer hair cell of cochlea CL:0000855
inner ear development GO:0048839
Show evidence (2 references)
PMID:38254937 SUPPORT Model Organism
"The KS1 mice also display diminished distortion product otoacoustic emission levels, which suggests outer hair cell dysfunction."
Demonstrates a cell-intrinsic outer hair cell mechanism for sensorineural hearing loss in KS1.
PMID:38254937 SUPPORT Human Clinical
"our data suggests that KMT2D dysfunction causes sensorineural hearing loss compounded with external factors, such as infection."
Supports a cell-intrinsic chromatin-based mechanism for the sensorineural hearing component of Kabuki syndrome.

Pathograph

Use the checkboxes to hide or show graph categories. Hover nodes for evidence and cross-linked metadata.
Pathograph: causal mechanism network for Kabuki Syndrome Interactive directed graph showing how pathophysiology mechanisms, phenotypes, genetic factors and variants, experimental models, environmental triggers, and treatments relate through causal and linked edges.

Phenotypes

28
Blood 1
Decreased Serum IgA VERY_FREQUENT Decreased circulating IgA concentration HP:0002720
Show evidence (2 references)
PMID:26194542 SUPPORT Human Clinical
"Among the patients with KMT2D mutations (KMT2D(Mut/+)), hypogammaglobulinemia was detected in all but 1 patient, with IgA deficiency affecting 90% of patients and a deficiency in at least 1 other isoform seen in 40% of patients."
Supports IgA deficiency as a prevalent immunologic phenotype.
ORPHA:2322 PARTIAL
"HP:0002720 | Decreased circulating IgA level | Occasional (29-5%)"
Orphanet classifies decreased circulating IgA as occasional (29-5%). This frequency estimate is lower than the 90% IgA deficiency rate reported in molecularly confirmed KMT2D cohorts (PMID:26194542), possibly reflecting ascertainment differences.
Cardiovascular 1
Congenital Heart Defects FREQUENT Abnormal heart morphology HP:0001627
Show evidence (2 references)
PMID:28884922 SUPPORT Human Clinical
"In conclusion, a CHD is detected in 70% of patients with KMT2D (MLL2) pathogenic variants, most commonly left-sided obstructive lesions, including multiple left-sided obstructions similar to those observed in the spectrum of the Shone complex, and septal defects."
Supports congenital heart disease as a frequent and clinically important manifestation.
ORPHA:2322 SUPPORT
"HP:0001627 | Abnormal heart morphology | Frequent (79-30%)"
Orphanet classifies abnormal heart morphology as frequent in Kabuki syndrome.
Digestive 2
Feeding Difficulties FREQUENT Feeding difficulties HP:0011968
Show evidence (1 reference)
ORPHA:2322 SUPPORT
"HP:0011968 | Feeding difficulties | Frequent (79-30%)"
Orphanet classifies feeding difficulties as frequent in Kabuki syndrome.
Gastroesophageal Reflux OCCASIONAL Gastroesophageal reflux HP:0002020
Show evidence (2 references)
ORPHA:2322 SUPPORT
"HP:0002020 | Gastroesophageal reflux | Occasional (29-5%)"
Orphanet classifies gastroesophageal reflux as occasional in Kabuki syndrome.
PMID:21882399 SUPPORT Human Clinical
"Thickened feedings and positioning after meals to treat gastroesophageal reflux"
GeneReviews recommends management of gastroesophageal reflux as part of Kabuki syndrome care.
Ear 2
Hearing Impairment FREQUENT Hearing impairment HP:0000365
Show evidence (6 references)
PMID:34570271 SUPPORT Human Clinical
"Kabuki syndrome (KS) is a genetic disorder caused mainly by de novo pathogenic variants in KMT2D or KDM6A, characterized by recognizable facial features, intellectual disability, and multi-systemic involvement, including short stature, microcephaly, hearing loss, cardiac defects, and additional..."
Identifies hearing loss as a recognized multisystem feature.
PMID:15523636 SUPPORT Human Clinical
"The presence or absence of hearing loss or major malformations, other than those involving the brain, was not predictive of developmental outcome."
Acknowledges hearing loss as a feature while noting it does not predict cognitive outcome.
PMID:38254937 SUPPORT Human Clinical
"individuals have both sensorineural and conductive hearing loss, with the average age of presentation being 7 years."
Documents the mixed conductive/sensorineural character of Kabuki hearing loss with mean age of presentation around 7 years.
+ 3 more references
Sensorineural Hearing Loss Sensorineural hearing impairment HP:0000407
Show evidence (2 references)
PMID:38254937 SUPPORT Human Clinical
"individuals have both sensorineural and conductive hearing loss, with the average age of presentation being 7 years."
Documents sensorineural hearing loss as a recurring component of the Kabuki hearing phenotype.
PMID:38254937 SUPPORT Model Organism
"The KS1 mice also display diminished distortion product otoacoustic emission levels, which suggests outer hair cell dysfunction."
Supports a cell-intrinsic outer hair cell mechanism for sensorineural hearing loss.
Eye 2
Strabismus FREQUENT Strabismus HP:0000486
Show evidence (2 references)
ORPHA:2322 SUPPORT
"HP:0000486 | Strabismus | Frequent (79-30%)"
Orphanet classifies strabismus as frequent in Kabuki syndrome.
PMID:21882399 SUPPORT Human Clinical
"ptosis and strabismus, and widely spaced teeth and hypodontia."
GeneReviews lists strabismus among recognized Kabuki syndrome findings.
Ptosis FREQUENT Ptosis HP:0000508
Show evidence (2 references)
ORPHA:2322 SUPPORT
"HP:0000508 | Ptosis | Frequent (79-30%)"
Orphanet classifies ptosis as frequent in Kabuki syndrome.
PMID:21882399 SUPPORT Human Clinical
"ptosis and strabismus, and widely spaced teeth and hypodontia."
GeneReviews lists ptosis among recognized Kabuki syndrome findings.
Genitourinary 2
Congenital Anomalies of the Kidney and Urinary Tract Abnormality of the urinary system HP:0000079
Show evidence (3 references)
PMID:34570271 SUPPORT Human Clinical
"CAKUT were detected in 8/13 (61.5%) of patients and varied from hypospadias, hydronephrosis, or double collecting systems to pelvic kidney, kidney asymmetry, horseshoe kidney, or kidney agenesis."
Demonstrates a high prevalence of CAKUT in a single-center cohort of KS patients.
PMID:21607748 SUPPORT Human Clinical
"MLL2 mutation carriers significantly more often presented with short stature and renal anomalies (p = 0.026 and 0.031, respectively)"
Renal anomalies are significantly associated with KMT2D mutation-positive patients.
ORPHA:2322 SUPPORT
"HP:0000079 | Abnormality of the urinary system | Frequent (79-30%)"
Orphanet classifies urinary system abnormalities as frequent in Kabuki syndrome.
Cryptorchidism OCCASIONAL Cryptorchidism HP:0000028
Show evidence (1 reference)
ORPHA:2322 SUPPORT
"HP:0000028 | Cryptorchidism | Occasional (29-5%)"
Orphanet classifies cryptorchidism as occasional in Kabuki syndrome.
Head and Neck 4
Long Palpebral Fissures Long palpebral fissure HP:0000637
Show evidence (2 references)
PMID:30514738 SUPPORT Human Clinical
"Typical dysmorphic features include long palpebral fissures with eversion of the lateral third of the lower eyelid and two or more of the following: (1) arched and broad eyebrows with the lateral third displaying notching or sparseness; (2) short columella with depressed nasal tip; (3) large,..."
Supports long palpebral fissures as a defining clinical feature.
ORPHA:2322 SUPPORT
"HP:0000637 | Long palpebral fissure | Frequent (79-30%)"
Orphanet classifies long palpebral fissures as frequent in Kabuki syndrome.
Arched Broad Eyebrows Highly arched eyebrow HP:0002553
Show evidence (2 references)
PMID:30514738 SUPPORT Human Clinical
"Typical dysmorphic features include long palpebral fissures with eversion of the lateral third of the lower eyelid and two or more of the following: (1) arched and broad eyebrows with the lateral third displaying notching or sparseness; (2) short columella with depressed nasal tip; (3) large,..."
Lists arched broad eyebrows as a primary diagnostic dysmorphic feature.
ORPHA:2322 SUPPORT
"HP:0002553 | Highly arched eyebrow | Very frequent (99-80%)"
Orphanet classifies highly arched eyebrows as very frequent in Kabuki syndrome.
Cleft Palate Cleft palate HP:0000175
Show evidence (4 references)
PMID:39202303 SUPPORT Human Clinical
"Other abnormalities included hearing loss (39.1%), seizures (26.1%), cleft palate (26.1%), and renal anomalies (21.7%)."
Documents cleft palate prevalence at 26.1% in a 23-patient KMT2D cohort.
PMID:32541010 SUPPORT Model Organism
"KMT2D mutant NCCs lead to defective secondary palatal shelf elevation with reduced expression of extracellular matrix components."
Identifies the developmental mechanism (palatal shelf elevation defect) underlying the cleft palate phenotype.
ORPHA:2322 SUPPORT
"HP:0000175 | Cleft palate | Frequent (79-30%)"
Orphanet classifies cleft palate as frequent in Kabuki syndrome.
+ 1 more reference
Abnormal Dental Morphology FREQUENT Abnormal dental morphology HP:0006482
Show evidence (2 references)
ORPHA:2322 SUPPORT
"HP:0006482 | Abnormal dental morphology | Frequent (79-30%)"
Orphanet classifies abnormal dental morphology as frequent in Kabuki syndrome.
PMID:21882399 SUPPORT Human Clinical
"widely spaced teeth and hypodontia."
GeneReviews lists dental anomalies among recognized Kabuki syndrome findings.
Immune 2
Recurrent Infections FREQUENT Recurrent infections HP:0002719
Show evidence (2 references)
PMID:31363182 SUPPORT Human Clinical
"Overall, 44.1% (78/177) and 58.2% (46/79) of KS individuals exhibited infection susceptibility and hypogammaglobulinemia, respectively;"
Supports recurrent infection susceptibility in a large cohort.
ORPHA:2322 SUPPORT
"HP:0002719 | Recurrent infections | Frequent (79-30%)"
Orphanet classifies recurrent infections as frequent in Kabuki syndrome.
Autoimmunity Autoimmunity HP:0002960
Show evidence (3 references)
PMID:31363182 SUPPORT Human Clinical
"13.6% (24/177) had autoimmune disease"
Quantifies autoimmune disease prevalence in a 177-patient KS registry.
PMID:31363182 SUPPORT Human Clinical
"The most frequent AID manifestations were immune thrombocytopenic purpura"
Identifies immune thrombocytopenic purpura as the most common autoimmune manifestation in KS.
PMID:31363182 SUPPORT Human Clinical
"Among nonhematological manifestations, vitiligo was frequent."
Documents vitiligo as a frequent non-hematological autoimmune manifestation in KS.
Integument 1
Prominent Fingertip Pads Prominent fingertip pads HP:0001212
Show evidence (2 references)
PMID:30514738 SUPPORT Human Clinical
"Typical dysmorphic features include long palpebral fissures with eversion of the lateral third of the lower eyelid and two or more of the following: (1) arched and broad eyebrows with the lateral third displaying notching or sparseness; (2) short columella with depressed nasal tip; (3) large,..."
Persistent fingertip pads are listed as one of the core diagnostic dysmorphic features.
ORPHA:2322 SUPPORT
"HP:0001212 | Prominent fingertip pads | Frequent (79-30%)"
Orphanet classifies prominent fingertip pads as frequent in Kabuki syndrome.
Musculoskeletal 3
Hypotonia FREQUENT Hypotonia HP:0001252
Show evidence (2 references)
PMID:30514738 SUPPORT Human Clinical
"The authors propose that a definitive diagnosis can be made in an individual of any age with a history of infantile hypotonia, developmental delay and/or intellectual disability, and one or both of the following major criteria: (1) a pathogenic or likely pathogenic variant in KMT2D or KDM6A; and..."
Supports hypotonia as a core early-life phenotype.
ORPHA:2322 SUPPORT
"HP:0001252 | Hypotonia | Frequent (79-30%)"
Orphanet classifies hypotonia as frequent in Kabuki syndrome.
Scoliosis Scoliosis HP:0002650
Show evidence (3 references)
PMID:33805950 SUPPORT Human Clinical
"hypertrichosis and scoliosis."
Lists scoliosis among the core skeletal manifestations of Kabuki syndrome in a clinical/molecular review.
PMID:11343317 PARTIAL Human Clinical
"Kabuki makeup (Niikawa-Kuroki) syndrome (KS) is characterized by distinct facial anomalies, mental retardation, congenital heart defect (CHD), and skeletal malformations."
Supports skeletal malformations broadly as a cardinal KS feature; scoliosis is a documented manifestation but not specifically named in this CHD-focused paper.
ORPHA:2322 SUPPORT
"HP:0002650 | Scoliosis | Frequent (79-30%)"
Orphanet classifies scoliosis as frequent in Kabuki syndrome.
Joint Hypermobility FREQUENT Joint hypermobility HP:0001382
Show evidence (1 reference)
ORPHA:2322 SUPPORT
"HP:0001382 | Joint hypermobility | Frequent (79-30%)"
Orphanet classifies joint hypermobility as frequent in Kabuki syndrome.
Nervous System 3
Intellectual Disability VERY_FREQUENT Intellectual disability HP:0001249
Show evidence (3 references)
PMID:30514738 SUPPORT Human Clinical
"The authors propose that a definitive diagnosis can be made in an individual of any age with a history of infantile hypotonia, developmental delay and/or intellectual disability, and one or both of the following major criteria: (1) a pathogenic or likely pathogenic variant in KMT2D or KDM6A; and..."
Places intellectual disability in the core diagnostic phenotype.
PMID:15523636 SUPPORT Human Clinical
"Based on these patients and a review of the literature, in the absence of major structural brain anomalies, the average intelligence quotient (IQ) in patients with this condition fall within the mild mental retardation range, however, specific developmental outcomes are widely variable, ranging..."
Supports the typical severity range and variable developmental outcome.
ORPHA:2322 SUPPORT
"HP:0001249 | Intellectual disability | Very frequent (99-80%)"
Orphanet classifies intellectual disability as very frequent in Kabuki syndrome.
Seizures OCCASIONAL Seizure HP:0001250
Show evidence (2 references)
PMID:39202303 SUPPORT Human Clinical
"Other abnormalities included hearing loss (39.1%), seizures (26.1%), cleft palate (26.1%), and renal anomalies (21.7%)."
Quantifies seizure prevalence at 26.1% in a 23-patient Taiwanese KS cohort.
ORPHA:2322 SUPPORT
"HP:0001250 | Seizure | Occasional (29-5%)"
Orphanet classifies seizures as occasional in Kabuki syndrome.
Speech Delay VERY_FREQUENT Delayed speech and language development HP:0000750
Show evidence (2 references)
PMID:39202303 SUPPORT Human Clinical
"The most common clinical characteristics included distinct facial features (100%), intellectual disability (100%), developmental delay (95.7%), speech delay (78.3%), hypotonia (69.6%), congenital heart abnormalities (69.6%), and recurrent infections (65.2%)."
Quantifies speech delay at 78.3% in a 23-patient Taiwanese KS cohort.
ORPHA:2322 SUPPORT
"HP:0012758 | Neurodevelopmental delay | Very frequent (99-80%)"
Orphanet classifies neurodevelopmental delay (encompassing speech delay) as very frequent in Kabuki syndrome.
Growth 3
Short Stature FREQUENT Short stature HP:0004322
Show evidence (3 references)
PMID:21607748 SUPPORT Human Clinical
"Kabuki syndrome (KS) is one of the classical, clinically well-known multiple anomalies/mental retardation syndromes, mainly characterized by a very distinctive facial appearance in combination with additional clinical signs such as developmental delay, short stature, persistent fingerpads, and..."
Supports short stature as a common core feature.
PMID:27649541 SUPPORT Human Clinical
"Five of the 18 children (27.8%) were biochemically GH deficient."
Documents biochemical GH deficiency in approximately one-quarter of KS children studied, supporting an endocrine contribution to short stature.
ORPHA:2322 SUPPORT
"HP:0004322 | Short stature | Frequent (79-30%)"
Orphanet classifies short stature as frequent in Kabuki syndrome.
Failure to Thrive FREQUENT Failure to thrive HP:0001508
Show evidence (2 references)
ORPHA:2322 SUPPORT
"HP:0001508 | Failure to thrive | Frequent (79-30%)"
Orphanet classifies failure to thrive as frequent in Kabuki syndrome.
PMID:15108197 SUPPORT Human Clinical
"Six of these eight patients had failure to thrive in infancy."
Documents failure to thrive in infancy in over half of patients studied.
Obesity OCCASIONAL Obesity HP:0001513
Show evidence (2 references)
ORPHA:2322 SUPPORT
"HP:0001513 | Obesity | Occasional (29-5%)"
Orphanet classifies obesity as occasional in Kabuki syndrome.
PMID:15108197 SUPPORT Human Clinical
"Eight of 14 patients over the age of 5 years were overweight or obese."
Documents the characteristic growth trajectory from infantile failure to thrive to later obesity.
Other 2
Hyperinsulinemic Hypoglycemia Hyperinsulinemic hypoglycemia HP:0000825
Show evidence (3 references)
PMID:29907798 SUPPORT Human Clinical
"The incidence of HI among patients with KS may be higher than previously reported, and KS may account for as much as 1% of patients diagnosed with HI."
Establishes congenital hyperinsulinism as a recognized presenting feature of Kabuki syndrome.
ORPHA:2322 SUPPORT
"HP:0000825 | Hyperinsulinemic hypoglycemia | Very rare (<4-1%)"
Orphanet classifies hyperinsulinemic hypoglycemia as very rare in Kabuki syndrome.
PMID:38859884 SUPPORT Human Clinical
"Hypoglycemia was recognized on the first day of life in 25 children (76%)."
Documents hyperinsulinism timing and prevalence in a 33-patient KS cohort, with 92% diazoxide responsiveness.
Premature Thelarche FREQUENT Premature thelarche HP:0010314
Show evidence (2 references)
ORPHA:2322 SUPPORT
"HP:0010314 | Premature thelarche | Frequent (79-30%)"
Orphanet classifies premature thelarche as frequent in Kabuki syndrome.
PMID:3067577 SUPPORT Human Clinical
"early breast development in infant girls (23%)"
Niikawa et al. documented premature thelarche in 23% of the original 62-patient cohort.
🧬

Genetic Associations

2
KMT2D (Causative)
Gene: KMT2D hgnc:7133
Show evidence (3 references)
PMID:20711175 SUPPORT Human Clinical
"Our results strongly suggest that mutations in MLL2 are a major cause of Kabuki syndrome."
Establishes KMT2D as the major causative gene.
PMID:24633898 SUPPORT In Vitro
"We found that a number of KMT2D truncating mutations result in mRNA degradation through the nonsense-mediated mRNA decay, contributing to protein haploinsufficiency."
Supports KMT2D haploinsufficiency as the dominant genetic mechanism.
ORPHA:2322 SUPPORT
"KMT2D | lysine methyltransferase 2D | hgnc:7133 | Disease-causing germline mutation(s) in"
Orphanet lists KMT2D as a disease-causing gene for Kabuki syndrome.
KDM6A (Causative)
Gene: KDM6A hgnc:12637
Show evidence (2 references)
PMID:22197486 SUPPORT Human Clinical
"Here, we describe three KS individuals with de novo partial or complete deletions of an X chromosome gene, KDM6A, that encodes a histone demethylase that interacts with MLL2."
Establishes KDM6A as a causative Kabuki syndrome gene.
ORPHA:2322 SUPPORT
"KDM6A | lysine demethylase 6A | hgnc:12637 | Disease-causing germline mutation(s) in"
Orphanet lists KDM6A as a disease-causing gene for Kabuki syndrome.
💊

Medical Actions

8
HDAC Inhibitor Therapy (Experimental)
Action: HDAC inhibitor therapy Ontology label: Pharmacotherapy NCIT:C15986
Histone deacetylase inhibitors such as AR-42 have shown preclinical promise in rescuing structural and functional brain deficits in Kmt2d haploinsufficient mouse models. By promoting histone acetylation, HDAC inhibitors can partially compensate for the reduced H3K4 methylation caused by KMT2D deficiency.
Mechanism Target:
RESTORES Postnatal neurogenesis deficit — HDAC inhibition increases histone acetylation, partially compensating for reduced H3K4 methylation and rescuing adult neurogenesis.
Show evidence (1 reference)
PMID:25273096 SUPPORT Model Organism
"These abnormalities improved upon postnatal treatment with AR-42."
Demonstrates rescue of neurogenesis-related abnormalities by HDAC inhibition.
Show evidence (1 reference)
PMID:25273096 SUPPORT Model Organism
"These abnormalities improved upon postnatal treatment with AR-42. Our work suggests that a reversible deficiency in postnatal neurogenesis underlies intellectual disability in Kabuki syndrome."
Demonstrates that HDAC inhibitor treatment can rescue neurogenesis and memory deficits in a Kabuki syndrome mouse model.
Growth Hormone Therapy
Action: Growth hormone replacement therapy Ontology label: human growth hormone replacement therapy MAXO:0000780
Growth hormone replacement may be considered for patients with documented growth hormone deficiency contributing to postnatal growth retardation.
Mechanism Target:
BYPASSES Chondrocyte differentiation defect — Exogenous GH/IGF-1 stimulation drives chondrocyte proliferation at the growth plate, partially bypassing the KMT2D/KDM6A-dependent enhancer defect in chondrocyte differentiation.
Show evidence (1 reference)
PMID:28793284 SUPPORT Human Clinical
"All participants experienced catch-up growth during the year of rhGH treatment, but without an influence on body proportions."
Supports recombinant human growth hormone as a treatment that can improve linear growth in children with Kabuki syndrome.
Speech Therapy
Action: Speech therapy Ontology label: speech therapy MAXO:0000930
Speech and language therapy is an important component of developmental support given the high prevalence of speech delay and intellectual disability.
Show evidence (1 reference)
PMID:15523636 PARTIAL Human Clinical
"Based on these patients and a review of the literature, in the absence of major structural brain anomalies, the average intelligence quotient (IQ) in patients with this condition fall within the mild mental retardation range, however, specific developmental outcomes are widely variable, ranging..."
Supports significant developmental variability warranting early intervention; speech therapy specifically is not discussed in this paper.
Cardiac Surgery
Action: Cardiac surgery Ontology label: surgical procedure on cardiovascular system MAXO:0025001
Surgical repair of congenital heart defects including septal defects, coarctation of the aorta, and other structural cardiac anomalies.
Mechanism Target:
BYPASSES Cardiomyocyte gene-expression dysregulation — Surgical repair corrects the structural consequence of cardiomyocyte and neural crest developmental defects without addressing the upstream chromatin mechanism.
BYPASSES Neural crest developmental dysregulation — Surgery addresses outflow-tract and septal defects produced by cardiac neural crest dysfunction.
Show evidence (1 reference)
PMID:11343317 SUPPORT Human Clinical
"CHD was diagnosed in 35 (58%) of our patients. Aortic coarctation (COA) (23%), atrial septal defect (ASD) (20%), and ventricular septal defect (VSD) (17%) were the most frequent CHDs"
High frequency of congenital heart defects necessitates surgical evaluation and repair.
Immunoglobulin Replacement
Action: Immunoglobulin replacement therapy Ontology label: immunoglobulin infusion therapy MAXO:0001480
Agent: therapeutic immune globulin NCIT:C2701
Intravenous or subcutaneous immunoglobulin replacement therapy for patients with severe hypogammaglobulinemia and recurrent infections.
Mechanism Target:
BYPASSES B-cell terminal differentiation defect — Exogenous immunoglobulin compensates for impaired endogenous antibody production from defective terminal B-cell differentiation.
Show evidence (1 reference)
PMID:15887282 PARTIAL Human Clinical
"Due to this increased susceptibility to infection, children with KS should have immunologic evaluations at the time of diagnosis in order to reduce preventable morbidity and mortality."
Supports the clinical need for immunologic work-up but does not explicitly recommend immunoglobulin replacement therapy.
KDM1A Inhibitor (Experimental)
Action: Pharmacotherapy NCIT:C15986
Lysine-specific demethylase 1A (KDM1A/LSD1) normally removes H3K4 methyl marks added by KMT2D. Pharmacologic KDM1A inhibition (e.g., TAK-418) rebalances H3K4 methylation, restores postnatal neurogenesis, and rescues hippocampal memory in Kmt2d-haploinsufficient mice. Targets the upstream chromatin defect more directly than HDAC inhibition.
Mechanism Target:
RESTORES Postnatal neurogenesis deficit — KDM1A inhibition restores the H3K4me-balance lost in KMT2D haploinsufficiency, rescuing adult hippocampal neurogenesis.
Show evidence (1 reference)
PMID:33738331 SUPPORT Model Organism
"After 2 weeks of TAK-418, Kmt2d +/βGeo mice demonstrated normalization of hippocampal memory defects."
Direct mechanism-targeted rescue of the neurogenesis-memory phenotype in a Kabuki mouse model.
Show evidence (1 reference)
PMID:33738331 SUPPORT Model Organism
"our data suggest that KDM1A inhibition is a plausible treatment strategy for KS"
Establishes KDM1A inhibition as a rational chromatin-targeted treatment strategy for Kabuki syndrome.
Modified Atkins Diet (Investigational)
Action: dietary intervention MAXO:0000088
Ketogenic/Modified Atkins dietary intervention is being investigated as a chromatin-modulating therapy in Kabuki syndrome. Multi-omics data show partial reversal of ribosomal protein dysregulation in a treated KMT2D KS patient. A completed 12-week adult phase-1 trial (NCT04722315) evaluated cognitive and methylation outcomes.
Mechanism Target:
MODULATES Chromatin dysregulation from KMT2D and KDM6A haploinsufficiency — Ketogenic state alters histone acetylation and chromatin accessibility, partially compensating for KMT2D-driven enhancer dysregulation.
Show evidence (1 reference)
PMID:38768529 PARTIAL Human Clinical
"A 12-year-old boy with KS, suffering from recurrent episodes of cognitive decline, exhibited improved cognitive function and neuropsychological assessment performance after 12 months on the ketogenic diet, with concomitant improvement in transcriptomic ribosomal protein dysregulation."
Demonstrates that ketogenic diet can modulate KMT2D-related ribosomal protein dysregulation with concurrent clinical improvement, supporting chromatin-modulating treatment targeting.
Diazoxide
Action: Pharmacotherapy NCIT:C15986
Agent: diazoxide CHEBI:4495
First-line pharmacotherapy for hyperinsulinemic hypoglycemia in Kabuki syndrome. Activates pancreatic beta-cell K-ATP channels to suppress insulin secretion. Approximately 92% of children with KS-associated hyperinsulinism respond to a diazoxide trial.
Mechanism Target:
INHIBITS Pancreatic beta-cell dysfunction — Diazoxide opens beta-cell K-ATP channels, suppressing dysregulated insulin secretion in KS-associated hyperinsulinism.
Show evidence (1 reference)
PMID:38859884 SUPPORT Human Clinical
"Diazoxide trial was conducted in 25 children, 92% of whom were responsive."
Demonstrates high clinical response rate to diazoxide for KS-associated hyperinsulinism.
Show evidence (1 reference)
PMID:38859884 SUPPORT Human Clinical
"HI treatment was discontinued in 46% of the cohort at median age 2.8 years"
Documents that medical management of KS-associated hyperinsulinism, primarily with diazoxide, frequently allows treatment discontinuation in early childhood.
📊

Related Datasets

1
The KMT2D Kabuki syndrome histone methylase controls neural crest cell differentiation and facial morphology geo:GSE149688
RNA-seq from E14.25 wild-type and Kmt2d neural crest cell knockout mouse palatal shelves, supporting analysis of KMT2D-dependent craniofacial developmental gene expression.
mouse BULK RNA SEQ
Conditions: E14.25 wild-type palatal shelves E14.25 Kmt2d neural crest cell knockout palatal shelves
PMID:32541010
GEO series linked to the KMT2D neural crest and palatal shelf model used to study craniofacial features of Kabuki syndrome.
Show evidence (1 reference)
PMID:32541010 PARTIAL Model Organism
"We mutated KMT2D in neural crest cells (NCCs) to study cellular and molecular functions in craniofacial development with respect to UTX."
Supports the mouse neural crest experimental context for this GEO RNA-seq dataset; the GEO accession identifies the deposited series.
🔬

Clinical Trials

1
NCT04722315 PHASE_I COMPLETED
Single-group early phase 1 trial evaluating a 12-week Modified Atkins Diet in adults with genetically confirmed Kabuki syndrome, with cognitive/visuospatial/memory outcomes plus serial genome-wide DNA methylation measurements.
Target Phenotypes: Intellectual disability HP:0001249
Show evidence (2 references)
clinicaltrials:NCT04722315 SUPPORT Human Clinical
"This study aims to examine a small number of adults with Kabuki syndrome before and after 12 weeks on a modified Atkins diet to determine if there is any cognitive improvement and if the diet can be tolerated."
Documents the trial's design and primary cognitive endpoint, anchoring the Modified Atkins Diet treatment in completed human evidence.
clinicaltrials:NCT04722315 SUPPORT Human Clinical
"Modified Atkins diet is safer and easier tolerated than full ketogenic diet and still has the histone deacetylase inhibition believed to be responsible for the cognitive improvement."
Provides the mechanistic rationale (HDAC inhibition via ketones) for testing Modified Atkins Diet in Kabuki syndrome.
{ }

Source YAML

click to show
name: Kabuki Syndrome
creation_date: '2026-03-15T23:04:41Z'
updated_date: '2026-04-30T20:04:42Z'
category: Mendelian
description: >-
  Kabuki syndrome is a multisystem Mendelian chromatin disorder caused primarily
  by heterozygous pathogenic variants in KMT2D and less commonly by pathogenic
  variants in KDM6A. The syndrome combines characteristic craniofacial
  dysmorphism, infantile hypotonia, developmental delay or intellectual
  disability, postnatal growth deficiency, congenital heart disease, and
  frequent humoral immune dysfunction. Its core mechanism is impaired epigenetic
  control of developmental gene expression with downstream effects on neural
  crest-derived tissues, skeletal growth, cardiovascular development, and B-cell
  maturation.
disease_term:
  preferred_term: Kabuki syndrome
  term:
    id: MONDO:0016512
    label: Kabuki syndrome
classifications:
  harrisons_chapter:
  - classification_value: GENETICS_ENVIRONMENT_DISEASE
    evidence:
    - reference: PMID:20711175
      reference_title: "Exome sequencing identifies MLL2 mutations as a cause of Kabuki syndrome."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        We demonstrate the successful application of exome sequencing to discover
        a gene for an autosomal dominant disorder, Kabuki syndrome
        (OMIM%147920).
      explanation: Supports classification as a hereditary genetic disorder.
  - classification_value: NEUROLOGIC
    evidence:
    - reference: PMID:30514738
      reference_title: "Kabuki syndrome: international consensus diagnostic criteria."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        The authors propose that a definitive diagnosis can be made in an
        individual of any age with a history of infantile hypotonia,
        developmental delay and/or intellectual disability, and one or both of
        the following major criteria: (1) a pathogenic or likely pathogenic
        variant in KMT2D or KDM6A; and (2) typical dysmorphic features (defined
        below) at some point of life.
      explanation: Supports classification as a neurodevelopmental/nervous system disorder.
parents:
- Neurodevelopmental Disorder
- Chromatin Disorder
- Multiple Congenital Anomaly Syndrome
synonyms:
- Kabuki make-up syndrome
- Niikawa-Kuroki syndrome
- KMS
definitions:
- name: International consensus clinical definition
  definition_type: CASE_DEFINITION
  description: >-
    Kabuki syndrome is clinically defined by infantile hypotonia with
    developmental delay or intellectual disability together with either a
    pathogenic KMT2D or KDM6A variant or the characteristic evolving Kabuki
    facial gestalt.
  scope: Molecularly confirmed and clinically recognizable Kabuki syndrome
  evidence:
  - reference: PMID:30514738
    reference_title: "Kabuki syndrome: international consensus diagnostic criteria."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The authors propose that a definitive diagnosis can be made in an
      individual of any age with a history of infantile hypotonia,
      developmental delay and/or intellectual disability, and one or both of
      the following major criteria: (1) a pathogenic or likely pathogenic
      variant in KMT2D or KDM6A; and (2) typical dysmorphic features (defined
      below) at some point of life.
    explanation: Supports a modern consensus case definition for Kabuki syndrome.
has_subtypes:
- name: KMT2D-related Kabuki syndrome
  subtype_term:
    preferred_term: Kabuki syndrome 1
    term:
      id: MONDO:0007843
      label: Kabuki syndrome 1
  classification: molecular
  description: >-
    Autosomal dominant Kabuki syndrome caused by pathogenic KMT2D variants and
    representing the major molecular subtype.
  genes:
  - preferred_term: KMT2D
    term:
      id: hgnc:7133
      label: KMT2D
  evidence:
  - reference: PMID:20711175
    reference_title: "Exome sequencing identifies MLL2 mutations as a cause of Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Our results strongly suggest that mutations in MLL2 are a major cause of
      Kabuki syndrome.
    explanation: Supports KMT2D as the principal disease gene and major subtype.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "KMT2D | lysine methyltransferase 2D | hgnc:7133 | Disease-causing germline mutation(s) in"
    explanation: Orphanet lists KMT2D as a disease-causing gene for Kabuki syndrome.
- name: KDM6A-related Kabuki syndrome
  subtype_term:
    preferred_term: Kabuki syndrome 2
    term:
      id: MONDO:0010465
      label: Kabuki syndrome 2
  classification: molecular
  description: >-
    Less common Kabuki syndrome subtype caused by pathogenic KDM6A variants.
  genes:
  - preferred_term: KDM6A
    term:
      id: hgnc:12637
      label: KDM6A
  evidence:
  - reference: PMID:22197486
    reference_title: "Deletion of KDM6A, a histone demethylase interacting with MLL2, in three patients with Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      This study identifies KDM6A mutations as another cause of KS and
      highlights the growing role of histone methylases and histone
      demethylases in multiple-congenital-anomaly and intellectual-disability
      syndromes.
    explanation: Supports a distinct KDM6A-related molecular subtype.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "KDM6A | lysine demethylase 6A | hgnc:12637 | Disease-causing germline mutation(s) in"
    explanation: Orphanet lists KDM6A as a disease-causing gene for Kabuki syndrome.
inheritance:
- name: Autosomal dominant inheritance
  inheritance_term:
    preferred_term: Autosomal dominant inheritance
    term:
      id: HP:0000006
      label: Autosomal dominant inheritance
  description: >-
    Most Kabuki syndrome cases are caused by heterozygous KMT2D variants and
    behave as an autosomal dominant disorder, usually arising de novo.
  evidence:
  - reference: PMID:20711175
    reference_title: "Exome sequencing identifies MLL2 mutations as a cause of Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      We demonstrate the successful application of exome sequencing to discover
      a gene for an autosomal dominant disorder, Kabuki syndrome
      (OMIM%147920).
    explanation: Supports autosomal dominant inheritance for the common KMT2D-related form.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "Autosomal dominant"
    explanation: Orphanet classifies Kabuki syndrome inheritance as autosomal dominant.
- name: X-linked dominant inheritance
  inheritance_term:
    preferred_term: X-linked dominant inheritance
    term:
      id: HP:0001423
      label: X-linked dominant inheritance
  description: >-
    A minority of cases are caused by heterozygous pathogenic variants or
    deletions in the X-linked gene KDM6A.
  evidence:
  - reference: PMID:22197486
    reference_title: "Deletion of KDM6A, a histone demethylase interacting with MLL2, in three patients with Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Here, we describe three KS individuals with de novo partial or complete
      deletions of an X chromosome gene, KDM6A, that encodes a histone
      demethylase that interacts with MLL2.
    explanation: Supports an X-linked disease mechanism for the KDM6A-related form.
pathophysiology:
- name: Chromatin dysregulation from KMT2D and KDM6A haploinsufficiency
  description: >-
    Kabuki syndrome is a disorder of epigenetic regulation in which loss of
    KMT2D or KDM6A function perturbs COMPASS-associated chromatin control.
    Truncating KMT2D variants frequently undergo nonsense-mediated decay,
    reducing KMT2D dosage and dysregulating developmental target genes.
    KDM6A lesions disrupt a cooperating histone demethylase in the same
    chromatin-regulatory pathway.
  cell_types:
  - preferred_term: neural crest cell
    term:
      id: CL:0011012
      label: neural crest cell
  - preferred_term: B cell
    term:
      id: CL:0000236
      label: B cell
  biological_processes:
  - preferred_term: chromatin organization
    term:
      id: GO:0006325
      label: chromatin organization
  - preferred_term: regulation of gene expression
    term:
      id: GO:0010468
      label: regulation of gene expression
  downstream:
  - target: Neural crest developmental dysregulation
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: >-
      KMT2D/KDM6A haploinsufficiency reduces enhancer H3K4me1 deposition
      and H3K27me3 demethylation, dysregulating developmental enhancers
      required for neural crest specification and migration.
  - target: Cardiomyocyte gene-expression dysregulation
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: >-
      Reduced KMT2D activity in cardiomyocytes lowers H3K4me1/me2 at
      enhancers controlling ion-transport and cell-cycle programs.
  - target: Chondrocyte differentiation defect
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: >-
      KMT2D/KDM6A loss disrupts H3K4me1 deposition at SOX9/RUNX2 enhancers
      controlling chondrogenic differentiation and growth-plate hypertrophy.
  - target: B-cell terminal differentiation defect
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: >-
      KMT2D loss alters enhancer activity at immune loci, including
      class-switch and integrin genes, impairing terminal B-cell maturation.
  - target: Postnatal neurogenesis deficit
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: >-
      Reduced KMT2D activity decreases dentate gyrus H3K4me3 and impairs
      adult hippocampal neurogenesis.
  - target: Pancreatic beta-cell dysfunction
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: >-
      KMT2D/KDM6A loss in pancreatic beta cells perturbs insulin secretion
      regulation, predisposing to congenital hyperinsulinism.
  - target: Cochlear outer hair cell dysfunction
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: >-
      KMT2D loss in cochlear hair cells contributes to sensorineural
      hearing loss independent of structural ear malformation.
  evidence:
  - reference: PMID:24633898
    reference_title: "Molecular analysis, pathogenic mechanisms, and readthrough therapy on a large cohort of Kabuki syndrome patients."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Kabuki syndrome (KS) is a multiple congenital anomalies syndrome
      characterized by characteristic facial features and varying degrees of
      mental retardation, caused by mutations in KMT2D/MLL2 and KDM6A/UTX
      genes.
    explanation: Establishes the two principal disease genes and the syndrome's developmental basis.
  - reference: PMID:24633898
    reference_title: "Molecular analysis, pathogenic mechanisms, and readthrough therapy on a large cohort of Kabuki syndrome patients."
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      We found that a number of KMT2D truncating mutations result in mRNA
      degradation through the nonsense-mediated mRNA decay, contributing to
      protein haploinsufficiency.
    explanation: Supports haploinsufficiency as a core molecular mechanism.
  - reference: PMID:22197486
    reference_title: "Deletion of KDM6A, a histone demethylase interacting with MLL2, in three patients with Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Here, we describe three KS individuals with de novo partial or complete
      deletions of an X chromosome gene, KDM6A, that encodes a histone
      demethylase that interacts with MLL2.
    explanation: Connects KDM6A loss to the same chromatin-regulatory disease mechanism.
  - reference: PMID:37043208
    reference_title: "Characterizing the molecular impact of KMT2D variants on the epigenetic and transcriptional landscapes in Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Our analysis identified unique enhancer signatures in H3K4me1 and
      H3K4me2 in KS compared with controls.
    explanation: Direct human evidence that KMT2D haploinsufficiency disrupts enhancer H3K4me1/me2 signatures in patient PBMCs.
  - reference: PMID:37043208
    reference_title: "Characterizing the molecular impact of KMT2D variants on the epigenetic and transcriptional landscapes in Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      31% of super-enhancers of normal blood cells overlapped with disrupted
      enhancers in KS, supporting an association of reduced enhancer activity
      of immune-related genes with immune deficiency phenotypes.
    explanation: Mechanistically links chromatin dysregulation to the immune phenotype via super-enhancer overlap.
  - reference: PMID:28669924
    reference_title: "Histone H3 lysine 4 methyltransferase KMT2D."
    supports: SUPPORT
    evidence_source: OTHER
    snippet: >-
      KMT2D is a major mammalian H3K4 mono-methyltransferase and co-localizes
      with lineage determining transcription factors on transcriptional
      enhancers. It is required for the binding of histone H3K27
      acetyltransferases CBP and p300 on enhancers, enhancer activation and
      cell-type specific gene expression during differentiation.
    explanation: Establishes KMT2D's molecular role as an H3K4 mono-methyltransferase coupling enhancer activation with H3K27 acetylation.
- name: Neural crest developmental dysregulation
  description: >-
    Experimental models indicate that Kabuki syndrome is a neurocristopathy.
    Impaired chromatin regulation in neural crest derivatives disrupts
    craniofacial morphogenesis, left-sided cardiovascular development, and
    postnatal growth, helping explain the characteristic facial gestalt,
    congenital heart disease, and growth deficiency seen clinically.
  cell_types:
  - preferred_term: neural crest cell
    term:
      id: CL:0011012
      label: neural crest cell
  - preferred_term: chondrocyte
    term:
      id: CL:0000138
      label: chondrocyte
  biological_processes:
  - preferred_term: neural crest cell migration
    term:
      id: GO:0001755
      label: neural crest cell migration
  - preferred_term: neural crest cell migration involved in heart formation
    term:
      id: GO:0003147
      label: neural crest cell migration involved in heart formation
  downstream:
  - target: Long Palpebral Fissures
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: Disrupted cranial neural crest patterning produces the elongated palpebral fissure phenotype.
  - target: Arched Broad Eyebrows
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: Altered cranial neural crest morphogenesis contributes to the characteristic eyebrow gestalt.
  - target: Cleft Palate
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: KMT2D loss in neural crest impairs secondary palatal shelf elevation and ECM expression, producing cleft palate.
  - target: Prominent Fingertip Pads
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: Persistent fetal fingertip pads are part of the broader neurocristopathy phenotype but specific molecular intermediates are not fully defined.
  - target: Congenital Heart Defects
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: Cardiac neural crest dysfunction contributes to outflow-tract and left-sided obstructive lesions.
    intermediate_mechanisms:
    - Impaired cardiac neural crest migration into outflow tract
    - Notch pathway dysregulation during endocardial patterning
  evidence:
  - reference: PMID:29073101
    reference_title: "UTX-guided neural crest function underlies craniofacial features of Kabuki syndrome."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      We now establish Kabuki syndrome as a neurocristopathy, whereby the
      majority of clinical features are modeled in mice carrying neural crest
      (NC) deletion of UTX, including craniofacial dysmorphism, cardiac
      defects, and postnatal growth retardation.
    explanation: Supports neural crest dysfunction as a unifying developmental mechanism.
  - reference: PMID:31479440
    reference_title: "Inhibition of Notch signaling rescues cardiovascular development in Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      Our zebrafish Kabuki Syndrome model reveals a regulatory link between the
      Notch pathway and Kmt2d during endothelium and endocardium patterning and
      shows that pharmacological inhibition of Notch signaling rebalances Rbpj
      protein levels and rescues the cardiovascular phenotype by enhancing
      endothelial and endocardial cell proliferation and stabilizing
      endocardial patterning.
    explanation: Supports a specific cardiovascular mechanism downstream of KMT2D deficiency.
  - reference: PMID:32541010
    reference_title: "The KMT2D Kabuki syndrome histone methylase controls neural crest cell differentiation and facial morphology."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      KMT2D NCC knockout mice demonstrate hypoplasia with reductions in
      frontonasal bone lengths.
    explanation: Establishes that conditional KMT2D loss in neural crest is sufficient to produce the craniofacial phenotype.
  - reference: PMID:32541010
    reference_title: "The KMT2D Kabuki syndrome histone methylase controls neural crest cell differentiation and facial morphology."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      KMT2D NCC loss-of-function does exhibit unique phenotypes distinct from
      UTX mutation, including fully penetrant cleft palate, mandible
      hypoplasia and deficits in cranial base ossification.
    explanation: Identifies KMT2D-specific neural crest phenotypes that distinguish KS1 from KS2.
  - reference: PMID:31813957
    reference_title: "The histone methyltransferase KMT2D, mutated in Kabuki syndrome patients, is required for neural crest cell formation and migration."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      Kmt2d knockdown correlates with a decrease in H3K4 monomethylation and
      H3K27 acetylation supporting a role of Kmt2d in the transcriptional
      activation of target genes.
    explanation: Provides the chromatin-mark mechanism (H3K4me1/H3K27ac loss) by which Kmt2d activates neural crest target genes.
  - reference: PMID:31813957
    reference_title: "The histone methyltransferase KMT2D, mutated in Kabuki syndrome patients, is required for neural crest cell formation and migration."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      Kmt2d loss-of-function inhibits Xenopus Sema3F expression, and
      overexpression of Sema3F can partially rescue Kmt2d loss-of-function
      defects.
    explanation: Identifies Sema3F as a downstream Kmt2d target gene whose restoration rescues neural crest defects.
  - reference: PMID:39327125
    reference_title: "KMT2D deficiency leads to cellular developmental disorders and enhancer dysregulation in neural-crest-containing brain organoids."
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      GREE identified that KMT2D targets a roof-plate-like niche cell and
      activates the niche cell-specific WNT3A enhancer, providing the
      microenvironment for neural crest and neuronal development.
    explanation: Identifies the WNT3A enhancer as a primary KMT2D target whose loss disrupts neural crest and neuronal development.
- name: B-cell terminal differentiation defect
  description: >-
    KMT2D deficiency impairs terminal B-cell maturation, class-switched memory
    B-cell formation, and humoral immune competence. This creates a clinically
    important immune phenotype with hypogammaglobulinemia, IgA deficiency,
    recurrent infections, and in a subset of patients autoimmune disease.
  cell_types:
  - preferred_term: B cell
    term:
      id: CL:0000236
      label: B cell
  biological_processes:
  - preferred_term: B cell differentiation
    term:
      id: GO:0030183
      label: B cell differentiation
  - preferred_term: somatic hypermutation of immunoglobulin genes
    term:
      id: GO:0016446
      label: somatic hypermutation of immunoglobulin genes
  downstream:
  - target: Decreased Serum IgA
    causal_link_type: DIRECT
    description: Impaired terminal B-cell maturation reduces class-switched IgA-producing plasma cells.
  - target: Recurrent Infections
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: Hypogammaglobulinemia and impaired class switching predispose to recurrent sinopulmonary and otologic infections.
    intermediate_mechanisms:
    - Reduced class-switched memory B cells
    - Hypogammaglobulinemia
  - target: Autoimmunity
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: B-cell tolerance and selection defects predispose a subset of patients to autoimmune cytopenias.
  evidence:
  - reference: PMID:26194542
    reference_title: "Defects of B-cell terminal differentiation in patients with type-1 Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Among the patients with KMT2D mutations (KMT2D(Mut/+)),
      hypogammaglobulinemia was detected in all but 1 patient, with IgA
      deficiency affecting 90% of patients and a deficiency in at least 1 other
      isoform seen in 40% of patients.
    explanation: Supports a high-burden humoral immunodeficiency phenotype in KMT2D-related disease.
  - reference: PMID:26194542
    reference_title: "Defects of B-cell terminal differentiation in patients with type-1 Kabuki syndrome."
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      Impaired terminal differentiation was noted in primary B cells from
      patients with KMT2D(Mut/+) mutations.
    explanation: Supports a cell-intrinsic B-cell maturation defect.
  - reference: PMID:31363182
    reference_title: "Immunopathological manifestations in Kabuki syndrome: a registry study of 177 individuals."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Overall, 44.1% (78/177) and 58.2% (46/79) of KS individuals exhibited
      infection susceptibility and hypogammaglobulinemia, respectively;
    explanation: Confirms that immune dysfunction and autoimmunity are common in a large registry cohort.
  - reference: PMID:38765012
    reference_title: "KMT2D regulates activation, localization, and integrin expression by T-cells."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      we reveal decreased expression (both at the transcriptional and
      translational levels) of a cluster of leukocyte-specific integrins,
      which perturb aspects of T-cell activation, maturation,
      adhesion/localization, and effector function.
    explanation: Extends the immune mechanism beyond B cells, showing KMT2D directly regulates leukocyte integrin expression and T-cell function.
  - reference: PMID:38765012
    reference_title: "KMT2D regulates activation, localization, and integrin expression by T-cells."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      H3K4me3 ChIP-PCR suggests that these evolutionary similar integrins are
      under direct control of KMT2D.
    explanation: Direct chromatin evidence (H3K4me3) for KMT2D control of integrin loci in lymphocytes.
  - reference: PMID:38765012
    reference_title: "KMT2D regulates activation, localization, and integrin expression by T-cells."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      KMT2D deficiency is
      associated with the accumulation of murine CD8+ single-positive (SP)
      thymocytes and shifts in both human and murine peripheral T-cell
      populations, including the reduction of the CD4+ recent thymic emigrant
      (RTE) population.
    explanation: Shows KMT2D loss perturbs T-cell development (CD8+ single-positive thymocyte accumulation and reduced CD4+ recent thymic emigrants), extending the immune mechanism to thymic T-cell maturation alongside the B-cell defect.
- name: Postnatal neurogenesis deficit
  description: >-
    Animal data support a chromatin-based neurodevelopmental mechanism in which
    reduced KMT2D activity decreases dentate gyrus H3K4me3, impairs postnatal
    neurogenesis, and contributes to hippocampal-dependent memory deficits.
  cell_types:
  - preferred_term: neuron
    term:
      id: CL:0000540
      label: neuron
  biological_processes:
  - preferred_term: neuron development
    term:
      id: GO:0048666
      label: neuron development
  downstream:
  - target: Intellectual Disability
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: Reduced adult hippocampal neurogenesis contributes to learning and memory deficits underlying intellectual disability.
    intermediate_mechanisms:
    - Reduced dentate gyrus H3K4me3
    - Decreased doublecortin-positive newborn neurons
  - target: Speech Delay
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: Neurodevelopmental chromatin disruption affecting postnatal neurogenesis contributes to delayed speech and language development beyond purely structural (hearing/oromotor) causes.
  - target: Seizures
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: Neuron-specific chromatin disruption may predispose a subset of patients to seizures; precise epileptogenic mechanism in Kabuki syndrome is not fully defined.
  evidence:
  - reference: PMID:25273096
    reference_title: "Histone deacetylase inhibition rescues structural and functional brain deficits in a mouse model of Kabuki syndrome."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      In vivo, deficiency of H3K4me3 in the dentate gyrus granule cell layer of
      Kmt2d(+/βGeo) mice correlated with reduced neurogenesis and hippocampal
      memory defects.
    explanation: Supports a mechanistic link between KMT2D-dependent chromatin defects and cognitive impairment.
  - reference: PMID:33738331
    reference_title: "Inhibition of KDM1A activity restores adult neurogenesis and improves hippocampal memory in a mouse model of Kabuki syndrome."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      orally administered TAK-418 increases the numbers of newly born
      doublecortin (DCX)+ cells and processes in the hippocampus in a
      dose-dependent manner.
    explanation: Confirms postnatal neurogenesis deficit can be reversed by restoring H3K4 methylation balance via KDM1A inhibition.
  - reference: PMID:38702196
    reference_title: "Neuron-specific chromatin disruption at CpG islands and aging-related regions in Kabuki syndrome mice."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      Neurons, but not B or T cells, show preferential chromatin disruption at
      CpG islands and at regulatory elements linked to aging.
    explanation: Demonstrates that neuronal chromatin disruption in Kabuki syndrome is cell-type specific and distinct from blood cell episignatures.
- name: Cardiomyocyte gene-expression dysregulation
  description: >-
    Beyond a neural-crest contribution, KMT2D acts cell-autonomously in cardiac
    precursors and cardiomyocytes during cardiogenesis. Myocardial Kmt2d
    deletion in mice reduces enhancer/promoter H3K4me1 and H3K4me2, downregulates
    ion-transport and cell-cycle genes, and produces altered calcium handling.
    Zebrafish models additionally implicate Notch dysregulation during
    endocardial patterning, contributing to congenital heart disease.
  cell_types:
  - preferred_term: cardiac muscle cell
    term:
      id: CL:0000746
      label: cardiac muscle cell
  biological_processes:
  - preferred_term: heart development
    term:
      id: GO:0007507
      label: heart development
  - preferred_term: Notch signaling pathway
    term:
      id: GO:0007219
      label: Notch signaling pathway
  - preferred_term: outflow tract morphogenesis
    term:
      id: GO:0003151
      label: outflow tract morphogenesis
  downstream:
  - target: Congenital Heart Defects
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: KMT2D-dependent enhancer activation in cardiomyocytes is required for normal cardiac gene expression; loss produces septal and outflow-tract defects.
    intermediate_mechanisms:
    - Reduced H3K4me1/me2 at cardiac enhancers
    - Dysregulated ion transport and cell-cycle gene expression
    - Notch pathway imbalance in endocardium
  evidence:
  - reference: PMID:26932671
    reference_title: "KMT2D regulates specific programs in heart development via histone H3 lysine 4 di-methylation."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      we demonstrate a requirement for KMT2D in cardiac precursors and
      cardiomyocytes during cardiogenesis in mice.
    explanation: Establishes cell-autonomous KMT2D requirement in cardiomyocytes during heart development.
  - reference: PMID:26932671
    reference_title: "KMT2D regulates specific programs in heart development via histone H3 lysine 4 di-methylation."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      myocardial Kmt2d deletion led to decreased H3K4me1 and H3K4me2 at
      enhancers and promoters.
    explanation: Demonstrates the chromatin-level molecular consequence of KMT2D loss in cardiomyocytes.
  - reference: PMID:31479440
    reference_title: "Inhibition of Notch signaling rescues cardiovascular development in Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      Our zebrafish Kabuki Syndrome model reveals a regulatory link between the
      Notch pathway and Kmt2d during endothelium and endocardium patterning
    explanation: Implicates Notch dysregulation as a downstream cardiovascular mechanism in Kabuki syndrome.
  - reference: PMID:31479440
    reference_title: "Inhibition of Notch signaling rescues cardiovascular development in Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      zebrafish kmt2d null mutants have robust Notch signaling hyperactivation in
      endocardial and endothelial cells, including increased protein levels of the
      Notch transcription factor Rbpj.
    explanation: Specifies the direction of the cardiovascular Notch defect - Kmt2d loss causes Notch pathway hyperactivation with elevated Rbpj in endocardial/endothelial cells, the substrate for the endocardial patterning defect.
- name: Chondrocyte differentiation defect
  description: >-
    KMT2D is required for hypertrophic chondrocyte differentiation and
    endochondral ossification. KS1 and KS2 cellular models exhibit precocious
    chondrocyte differentiation, shortened growth-plate hypertrophic zones,
    and convergent transcriptomic dysregulation downstream of SOX9 and RUNX2
    enhancer programs. This contributes to postnatal growth deficiency,
    skeletal anomalies, and cleft palate via cranial-base ossification defects.
  cell_types:
  - preferred_term: chondrocyte
    term:
      id: CL:0000138
      label: chondrocyte
  - preferred_term: hypertrophic chondrocyte
    term:
      id: CL:0000138
      label: chondrocyte
  biological_processes:
  - preferred_term: chondrocyte differentiation
    term:
      id: GO:0002062
      label: chondrocyte differentiation
  - preferred_term: endochondral ossification
    term:
      id: GO:0001958
      label: endochondral ossification
  downstream:
  - target: Short Stature
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: Premature/dysregulated chondrocyte differentiation and shortened growth-plate hypertrophic zones contribute to postnatal growth deficiency.
  - target: Cleft Palate
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: Cranial-base chondrocyte differentiation defects impair endochondral ossification and palatal shelf elevation.
  - target: Scoliosis
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: Generalized chondrocyte differentiation defects contribute to skeletal anomalies including scoliosis.
  evidence:
  - reference: PMID:38857303
    reference_title: "Growth deficiency in a mouse model of Kabuki syndrome 2 bears mechanistic similarities to Kabuki syndrome 1."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      Kdm6atm1d/+ growth plates are also shorter, due to decreases in
      hypertrophic chondrocyte size and hypertrophic zone height.
    explanation: Demonstrates a growth-plate hypertrophic chondrocyte defect underlying KS2 short stature.
  - reference: PMID:38857303
    reference_title: "Growth deficiency in a mouse model of Kabuki syndrome 2 bears mechanistic similarities to Kabuki syndrome 1."
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      RNA-seq simultaneously on Kmt2d-/-, Kdm6a-/-, and control lines at Days 7
      and 14 of differentiation. This revealed surprising resemblance in gene
      expression between Kmt2d-/- and Kdm6a-/-
    explanation: Supports a convergent KS1/KS2 chondrocyte transcriptomic program.
  - reference: PMID:38857303
    reference_title: "Growth deficiency in a mouse model of Kabuki syndrome 2 bears mechanistic similarities to Kabuki syndrome 1."
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      we found that Kdm6a-/- cells undergo premature, enhanced
      differentiation towards chondrocytes compared to Kdm6a+/+ controls.
    explanation: Direct in vitro evidence that KDM6A loss drives premature, enhanced chondrocyte differentiation, mirroring the precocious differentiation seen in KS1 Kmt2d-/- chondrocytes and underlying the growth-plate defect.
  - reference: PMID:32541010
    reference_title: "The KMT2D Kabuki syndrome histone methylase controls neural crest cell differentiation and facial morphology."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      KMT2D mutant chondrocytes in the cranial base fail to properly
      differentiate, leading to defective endochondral ossification.
    explanation: Connects KMT2D loss in chondrocytes to cranial-base ossification defects.
- name: Pancreatic beta-cell dysfunction
  description: >-
    Pathogenic variants in KMT2D and KDM6A predispose to congenital
    hyperinsulinism through dysregulated insulin secretion in pancreatic
    beta cells. Hypoglycemia frequently presents in the neonatal period and
    is typically diazoxide-responsive.
  cell_types:
  - preferred_term: pancreatic beta cell
    term:
      id: CL:0000169
      label: type B pancreatic cell
  biological_processes:
  - preferred_term: insulin secretion
    term:
      id: GO:0030073
      label: insulin secretion
  - preferred_term: regulation of insulin secretion
    term:
      id: GO:0050796
      label: regulation of insulin secretion
  downstream:
  - target: Hyperinsulinemic Hypoglycemia
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: Dysregulated insulin secretion in KMT2D/KDM6A-deficient beta cells produces persistent neonatal hypoglycemia.
  evidence:
  - reference: PMID:29907798
    reference_title: "Congenital hyperinsulinism as the presenting feature of Kabuki syndrome: clinical and molecular characterization of 9 affected individuals."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Molecular diagnoses of KS were established by identification of
      pathogenic variants in KMT2D (n = 5) and KDM6A (n = 4).
    explanation: Confirms that both KMT2D and KDM6A loss can present with hyperinsulinism, supporting a shared beta-cell mechanism.
  - reference: PMID:38859884
    reference_title: "Clinical and Molecular Characterization of Hyperinsulinism in Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Diazoxide trial was conducted in 25 children, 92% of whom were
      responsive.
    explanation: Demonstrates that hyperinsulinism in KS is typically diazoxide-responsive, consistent with a regulatory rather than structural beta-cell defect.
- name: Cochlear outer hair cell dysfunction
  description: >-
    KMT2D loss causes cell-intrinsic cochlear outer hair cell dysfunction,
    contributing to sensorineural hearing loss in addition to the conductive
    component from recurrent otitis media. Mouse models show diminished
    distortion product otoacoustic emissions despite no gross cochlear
    structural malformation on micro-CT.
  cell_types:
  - preferred_term: outer hair cell of cochlea
    term:
      id: CL:0000855
      label: sensory hair cell
  biological_processes:
  - preferred_term: inner ear development
    term:
      id: GO:0048839
      label: inner ear development
  downstream:
  - target: Sensorineural Hearing Loss
    causal_link_type: DIRECT
    description: KMT2D loss in cochlear hair cells produces cell-intrinsic outer hair cell dysfunction underlying sensorineural hearing loss.
  - target: Hearing Impairment
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    description: Combined sensorineural (hair cell) and conductive (recurrent otitis media) components produce the overall Kabuki hearing impairment phenotype.
  evidence:
  - reference: PMID:38254937
    reference_title: "KMT2D Deficiency Causes Sensorineural Hearing Loss in Mice and Humans."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      The KS1 mice also display diminished distortion product otoacoustic
      emission levels, which suggests outer hair cell dysfunction.
    explanation: Demonstrates a cell-intrinsic outer hair cell mechanism for sensorineural hearing loss in KS1.
  - reference: PMID:38254937
    reference_title: "KMT2D Deficiency Causes Sensorineural Hearing Loss in Mice and Humans."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      our data suggests that KMT2D dysfunction causes sensorineural hearing
      loss compounded with external factors, such as infection.
    explanation: Supports a cell-intrinsic chromatin-based mechanism for the sensorineural hearing component of Kabuki syndrome.
phenotypes:
- name: Long Palpebral Fissures
  category: Craniofacial
  diagnostic: true
  description: >-
    Long palpebral fissures with eversion of the lateral lower eyelid are a core
    component of the characteristic Kabuki facial gestalt.
  phenotype_term:
    preferred_term: Long palpebral fissure
    term:
      id: HP:0000637
      label: Long palpebral fissure
  evidence:
  - reference: PMID:30514738
    reference_title: "Kabuki syndrome: international consensus diagnostic criteria."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Typical dysmorphic features include long palpebral fissures with eversion
      of the lateral third of the lower eyelid and two or more of the
      following: (1) arched and broad eyebrows with the lateral third
      displaying notching or sparseness; (2) short columella with depressed
      nasal tip; (3) large, prominent or cupped ears; and (4) persistent
      fingertip pads.
    explanation: Supports long palpebral fissures as a defining clinical feature.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0000637 | Long palpebral fissure | Frequent (79-30%)"
    explanation: Orphanet classifies long palpebral fissures as frequent in Kabuki syndrome.
- name: Arched Broad Eyebrows
  category: Craniofacial
  diagnostic: true
  description: >-
    Arched and broad eyebrows with lateral third notching or sparseness are a
    core component of the characteristic Kabuki facial gestalt.
  phenotype_term:
    preferred_term: Arched broad eyebrows
    term:
      id: HP:0002553
      label: Highly arched eyebrow
  evidence:
  - reference: PMID:30514738
    reference_title: "Kabuki syndrome: international consensus diagnostic criteria."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Typical dysmorphic features include long palpebral fissures with eversion
      of the lateral third of the lower eyelid and two or more of the
      following: (1) arched and broad eyebrows with the lateral third
      displaying notching or sparseness; (2) short columella with depressed
      nasal tip; (3) large, prominent or cupped ears; and (4) persistent
      fingertip pads.
    explanation: Lists arched broad eyebrows as a primary diagnostic dysmorphic feature.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0002553 | Highly arched eyebrow | Very frequent (99-80%)"
    explanation: Orphanet classifies highly arched eyebrows as very frequent in Kabuki syndrome.
- name: Intellectual Disability
  category: Neurological
  frequency: VERY_FREQUENT
  description: >-
    Developmental delay and usually mild-to-moderate intellectual disability are
    central neurodevelopmental manifestations.
  phenotype_term:
    preferred_term: Intellectual disability
    term:
      id: HP:0001249
      label: Intellectual disability
  evidence:
  - reference: PMID:30514738
    reference_title: "Kabuki syndrome: international consensus diagnostic criteria."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The authors propose that a definitive diagnosis can be made in an
      individual of any age with a history of infantile hypotonia,
      developmental delay and/or intellectual disability, and one or both of
      the following major criteria: (1) a pathogenic or likely pathogenic
      variant in KMT2D or KDM6A; and (2) typical dysmorphic features (defined
      below) at some point of life.
    explanation: Places intellectual disability in the core diagnostic phenotype.
  - reference: PMID:15523636
    reference_title: "Developmental outcome in Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Based on these patients and a review of the literature, in the absence of
      major structural brain anomalies, the average intelligence quotient (IQ)
      in patients with this condition fall within the mild mental retardation
      range, however, specific developmental outcomes are widely variable,
      ranging from severe MR to normal intelligence.
    explanation: Supports the typical severity range and variable developmental outcome.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0001249 | Intellectual disability | Very frequent (99-80%)"
    explanation: Orphanet classifies intellectual disability as very frequent in Kabuki syndrome.
- name: Short Stature
  category: Growth
  frequency: FREQUENT
  description: >-
    Postnatal growth deficiency commonly leads to short stature. About 28%
    of KS children meet biochemical criteria for growth hormone deficiency,
    and growth-plate hypertrophic chondrocyte abnormalities are documented
    in both KS1 and KS2 mouse models.
  phenotype_term:
    preferred_term: Short stature
    term:
      id: HP:0004322
      label: Short stature
  evidence:
  - reference: PMID:21607748
    reference_title: "A mutation screen in patients with Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Kabuki syndrome (KS) is one of the classical, clinically well-known
      multiple anomalies/mental retardation syndromes, mainly characterized by
      a very distinctive facial appearance in combination with additional
      clinical signs such as developmental delay, short stature, persistent
      fingerpads, and urogenital tract anomalies.
    explanation: Supports short stature as a common core feature.
  - reference: PMID:27649541
    reference_title: "Growth Hormone Stimulation Tests in Children with Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Five of the 18 children (27.8%) were biochemically GH deficient.
    explanation: Documents biochemical GH deficiency in approximately one-quarter of KS children studied, supporting an endocrine contribution to short stature.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0004322 | Short stature | Frequent (79-30%)"
    explanation: Orphanet classifies short stature as frequent in Kabuki syndrome.
- name: Hypotonia
  category: Neurological
  frequency: FREQUENT
  description: >-
    Infantile hypotonia is part of the consensus clinical definition and
    contributes to motor delay and feeding difficulties. Reported in 69.6%
    of a Taiwanese KMT2D cohort.
  phenotype_term:
    preferred_term: Hypotonia
    term:
      id: HP:0001252
      label: Hypotonia
  evidence:
  - reference: PMID:30514738
    reference_title: "Kabuki syndrome: international consensus diagnostic criteria."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The authors propose that a definitive diagnosis can be made in an
      individual of any age with a history of infantile hypotonia,
      developmental delay and/or intellectual disability, and one or both of
      the following major criteria: (1) a pathogenic or likely pathogenic
      variant in KMT2D or KDM6A; and (2) typical dysmorphic features (defined
      below) at some point of life.
    explanation: Supports hypotonia as a core early-life phenotype.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0001252 | Hypotonia | Frequent (79-30%)"
    explanation: Orphanet classifies hypotonia as frequent in Kabuki syndrome.
- name: Congenital Heart Defects
  category: Cardiovascular
  frequency: FREQUENT
  description: >-
    Congenital heart disease is common in Kabuki syndrome, especially
    left-sided obstructive lesions and septal defects. Detected in 70% of
    KMT2D-positive patients in one cohort and 69.6% in a Taiwanese
    23-patient series.
  phenotype_term:
    preferred_term: Abnormal heart morphology
    term:
      id: HP:0001627
      label: Abnormal heart morphology
  evidence:
  - reference: PMID:28884922
    reference_title: "Congenital heart defects in molecularly proven Kabuki syndrome patients."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      In conclusion, a CHD is detected in 70% of patients with KMT2D (MLL2)
      pathogenic variants, most commonly left-sided obstructive lesions,
      including multiple left-sided obstructions similar to those observed in
      the spectrum of the Shone complex, and septal defects.
    explanation: Supports congenital heart disease as a frequent and clinically important manifestation.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0001627 | Abnormal heart morphology | Frequent (79-30%)"
    explanation: Orphanet classifies abnormal heart morphology as frequent in Kabuki syndrome.
- name: Decreased Serum IgA
  category: Immunological
  frequency: VERY_FREQUENT
  description: >-
    Humoral immune dysfunction often includes decreased serum IgA levels with
    broader hypogammaglobulinemia in a subset of patients. IgA deficiency
    affects 90% of KMT2D-positive patients in a French cohort, and
    hypogammaglobulinemia 58.2% in a 177-patient registry.
  phenotype_term:
    preferred_term: Decreased serum IgA
    term:
      id: HP:0002720
      label: Decreased circulating IgA concentration
  evidence:
  - reference: PMID:26194542
    reference_title: "Defects of B-cell terminal differentiation in patients with type-1 Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Among the patients with KMT2D mutations (KMT2D(Mut/+)),
      hypogammaglobulinemia was detected in all but 1 patient, with IgA
      deficiency affecting 90% of patients and a deficiency in at least 1 other
      isoform seen in 40% of patients.
    explanation: Supports IgA deficiency as a prevalent immunologic phenotype.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: PARTIAL
    snippet: "HP:0002720 | Decreased circulating IgA level | Occasional (29-5%)"
    explanation: >-
      Orphanet classifies decreased circulating IgA as occasional (29-5%).
      This frequency estimate is lower than the 90% IgA deficiency rate
      reported in molecularly confirmed KMT2D cohorts (PMID:26194542),
      possibly reflecting ascertainment differences.
- name: Recurrent Infections
  category: Immunological
  frequency: FREQUENT
  description: >-
    Recurrent sinopulmonary and otologic infections are common clinical
    consequences of the associated antibody deficiency. Reported in 44.1%
    of a 177-patient KS registry and 65.2% of a 23-patient Taiwanese
    cohort.
  phenotype_term:
    preferred_term: Recurrent infections
    term:
      id: HP:0002719
      label: Recurrent infections
  evidence:
  - reference: PMID:31363182
    reference_title: "Immunopathological manifestations in Kabuki syndrome: a registry study of 177 individuals."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Overall, 44.1% (78/177) and 58.2% (46/79) of KS individuals exhibited
      infection susceptibility and hypogammaglobulinemia, respectively;
    explanation: Supports recurrent infection susceptibility in a large cohort.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0002719 | Recurrent infections | Frequent (79-30%)"
    explanation: Orphanet classifies recurrent infections as frequent in Kabuki syndrome.
- name: Prominent Fingertip Pads
  category: Musculoskeletal
  diagnostic: true
  description: >-
    Persistent fetal fingertip pads are a hallmark feature included in the
    consensus diagnostic criteria.
  phenotype_term:
    preferred_term: Prominent fingertip pads
    term:
      id: HP:0001212
      label: Prominent fingertip pads
  evidence:
  - reference: PMID:30514738
    reference_title: "Kabuki syndrome: international consensus diagnostic criteria."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Typical dysmorphic features include long palpebral fissures with eversion
      of the lateral third of the lower eyelid and two or more of the
      following: (1) arched and broad eyebrows with the lateral third
      displaying notching or sparseness; (2) short columella with depressed
      nasal tip; (3) large, prominent or cupped ears; and (4) persistent
      fingertip pads.
    explanation: Persistent fingertip pads are listed as one of the core diagnostic dysmorphic features.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0001212 | Prominent fingertip pads | Frequent (79-30%)"
    explanation: Orphanet classifies prominent fingertip pads as frequent in Kabuki syndrome.
- name: Scoliosis
  category: Musculoskeletal
  description: >-
    Skeletal anomalies including scoliosis are well-documented manifestations
    of Kabuki syndrome, linked to altered neural crest and chondrocyte
    development.
  phenotype_term:
    preferred_term: Scoliosis
    term:
      id: HP:0002650
      label: Scoliosis
  evidence:
  - reference: PMID:33805950
    reference_title: "Kabuki Syndrome-Clinical Review with Molecular Aspects."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      hypertrichosis and scoliosis.
    explanation: Lists scoliosis among the core skeletal manifestations of Kabuki syndrome in a clinical/molecular review.
  - reference: PMID:11343317
    reference_title: "Congenital heart defects in Kabuki syndrome."
    supports: PARTIAL
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Kabuki makeup (Niikawa-Kuroki) syndrome (KS) is characterized by
      distinct facial anomalies, mental retardation, congenital heart defect
      (CHD), and skeletal malformations.
    explanation: >-
      Supports skeletal malformations broadly as a cardinal KS feature;
      scoliosis is a documented manifestation but not specifically named
      in this CHD-focused paper.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0002650 | Scoliosis | Frequent (79-30%)"
    explanation: Orphanet classifies scoliosis as frequent in Kabuki syndrome.
- name: Hearing Impairment
  category: Otological
  frequency: FREQUENT
  description: >-
    Hearing loss is common in Kabuki syndrome and combines conductive
    components (driven by recurrent otitis media) with cell-intrinsic
    sensorineural components (cochlear outer hair cell dysfunction). Mean
    age of presentation is approximately 7 years; reported in 39.1% of a
    Taiwanese cohort.
  phenotype_term:
    preferred_term: Hearing impairment
    term:
      id: HP:0000365
      label: Hearing impairment
  evidence:
  - reference: PMID:34570271
    reference_title: "Kidney and urinary tract findings among patients with Kabuki (make-up) syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Kabuki syndrome (KS) is a genetic disorder caused mainly by de novo
      pathogenic variants in KMT2D or KDM6A, characterized by recognizable
      facial features, intellectual disability, and multi-systemic involvement,
      including short stature, microcephaly, hearing loss, cardiac defects, and
      additional congenital anomalies.
    explanation: Identifies hearing loss as a recognized multisystem feature.
  - reference: PMID:15523636
    reference_title: "Developmental outcome in Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The presence or absence of hearing loss or major malformations, other
      than those involving the brain, was not predictive of developmental
      outcome.
    explanation: Acknowledges hearing loss as a feature while noting it does not predict cognitive outcome.
  - reference: PMID:38254937
    reference_title: "KMT2D Deficiency Causes Sensorineural Hearing Loss in Mice and Humans."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      individuals have both sensorineural and conductive hearing loss, with
      the average age of presentation being 7 years.
    explanation: Documents the mixed conductive/sensorineural character of Kabuki hearing loss with mean age of presentation around 7 years.
  - reference: PMID:39202303
    reference_title: "Genetic and Phenotypic Spectrum of KMT2D Variants in Taiwanese Case Series of Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Other abnormalities included hearing loss (39.1%), seizures (26.1%),
      cleft palate (26.1%), and renal anomalies (21.7%).
    explanation: Quantifies hearing loss prevalence at 39.1% in a 23-patient Taiwanese KMT2D cohort.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0000405 | Conductive hearing impairment | Frequent (79-30%)"
    explanation: Orphanet classifies conductive hearing impairment as frequent in Kabuki syndrome.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0000407 | Sensorineural hearing impairment | Frequent (79-30%)"
    explanation: Orphanet classifies sensorineural hearing impairment as frequent in Kabuki syndrome.
- name: Cleft Palate
  category: Craniofacial
  description: >-
    Cleft palate occurs in a subset of patients, consistent with the
    neurocristopathy and chondrocyte-differentiation mechanisms. Reported
    at 26.1% in a Taiwanese cohort. Mouse models with conditional KMT2D
    loss in neural crest exhibit fully penetrant cleft palate driven by
    defective secondary palatal shelf elevation and cranial-base
    ossification deficits.
  phenotype_term:
    preferred_term: Cleft palate
    term:
      id: HP:0000175
      label: Cleft palate
  evidence:
  - reference: PMID:39202303
    reference_title: "Genetic and Phenotypic Spectrum of KMT2D Variants in Taiwanese Case Series of Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Other abnormalities included hearing loss (39.1%), seizures (26.1%),
      cleft palate (26.1%), and renal anomalies (21.7%).
    explanation: Documents cleft palate prevalence at 26.1% in a 23-patient KMT2D cohort.
  - reference: PMID:32541010
    reference_title: "The KMT2D Kabuki syndrome histone methylase controls neural crest cell differentiation and facial morphology."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      KMT2D mutant NCCs lead to defective secondary palatal shelf elevation
      with reduced expression of extracellular matrix components.
    explanation: Identifies the developmental mechanism (palatal shelf elevation defect) underlying the cleft palate phenotype.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0000175 | Cleft palate | Frequent (79-30%)"
    explanation: Orphanet classifies cleft palate as frequent in Kabuki syndrome.
  - reference: PMID:17105332
    reference_title: "Cleft palate in Kabuki syndrome: a report of six cases."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Although cleft palate is a feature that is sometimes observed in patients
      with Kabuki syndrome, there are few clinical reports of cleft palate
      associated with Kabuki syndrome.
    explanation: Supports cleft palate as a recognized craniofacial manifestation of Kabuki syndrome.
- name: Congenital Anomalies of the Kidney and Urinary Tract
  category: Genitourinary
  description: >-
    Renal and urinary tract malformations are frequent, ranging from
    hydronephrosis and double collecting systems to horseshoe kidney
    and kidney agenesis.
  phenotype_term:
    preferred_term: Abnormality of the urinary system
    term:
      id: HP:0000079
      label: Abnormality of the urinary system
  evidence:
  - reference: PMID:34570271
    reference_title: "Kidney and urinary tract findings among patients with Kabuki (make-up) syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      CAKUT were detected in 8/13 (61.5%) of patients and varied from
      hypospadias, hydronephrosis, or double collecting systems to pelvic
      kidney, kidney asymmetry, horseshoe kidney, or kidney agenesis.
    explanation: Demonstrates a high prevalence of CAKUT in a single-center cohort of KS patients.
  - reference: PMID:21607748
    reference_title: "A mutation screen in patients with Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      MLL2 mutation carriers significantly more often presented with short
      stature and renal anomalies (p = 0.026 and 0.031, respectively)
    explanation: Renal anomalies are significantly associated with KMT2D mutation-positive patients.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0000079 | Abnormality of the urinary system | Frequent (79-30%)"
    explanation: Orphanet classifies urinary system abnormalities as frequent in Kabuki syndrome.
- name: Autoimmunity
  category: Immunological
  description: >-
    A subset of patients develop autoimmune manifestations including
    immune thrombocytopenic purpura, autoimmune hemolytic anemia, and
    vitiligo, reflecting the paradox of concurrent immunodeficiency and
    immune dysregulation. Registry data quantify autoimmune disease in
    13.6% overall and 25.6% of adults.
  phenotype_term:
    preferred_term: Autoimmunity
    term:
      id: HP:0002960
      label: Autoimmunity
  evidence:
  - reference: PMID:31363182
    reference_title: "Immunopathological manifestations in Kabuki syndrome: a registry study of 177 individuals."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      13.6% (24/177) had autoimmune disease
    explanation: Quantifies autoimmune disease prevalence in a 177-patient KS registry.
  - reference: PMID:31363182
    reference_title: "Immunopathological manifestations in Kabuki syndrome: a registry study of 177 individuals."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The most frequent AID manifestations were immune
      thrombocytopenic purpura
    explanation: Identifies immune thrombocytopenic purpura as the most common autoimmune manifestation in KS.
  - reference: PMID:31363182
    reference_title: "Immunopathological manifestations in Kabuki syndrome: a registry study of 177 individuals."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Among nonhematological manifestations, vitiligo was frequent.
    explanation: Documents vitiligo as a frequent non-hematological autoimmune manifestation in KS.
- name: Hyperinsulinemic Hypoglycemia
  category: Endocrine
  description: >-
    Congenital hyperinsulinism causing persistent neonatal hypoglycemia is a
    recognized presenting feature of Kabuki syndrome. Reported in both KMT2D
    and KDM6A patients, frequently diazoxide-responsive.
  phenotype_term:
    preferred_term: Hyperinsulinemic hypoglycemia
    term:
      id: HP:0000825
      label: Hyperinsulinemic hypoglycemia
  evidence:
  - reference: PMID:29907798
    reference_title: "Congenital hyperinsulinism as the presenting feature of Kabuki syndrome: clinical and molecular characterization of 9 affected individuals."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The incidence of HI among patients with KS may be higher than
      previously reported, and KS may account for as much as 1% of patients
      diagnosed with HI.
    explanation: Establishes congenital hyperinsulinism as a recognized presenting feature of Kabuki syndrome.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0000825 | Hyperinsulinemic hypoglycemia | Very rare (<4-1%)"
    explanation: Orphanet classifies hyperinsulinemic hypoglycemia as very rare in Kabuki syndrome.
  - reference: PMID:38859884
    reference_title: "Clinical and Molecular Characterization of Hyperinsulinism in Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Hypoglycemia was recognized on the first day of life in 25 children
      (76%).
    explanation: Documents hyperinsulinism timing and prevalence in a 33-patient KS cohort, with 92% diazoxide responsiveness.
- name: Sensorineural Hearing Loss
  category: Otological
  description: >-
    Sensorineural hearing loss is documented in Kabuki syndrome and arises
    from cochlear hair cell dysfunction independent of recurrent otitis
    media or structural ear anomalies. Mouse models show diminished
    distortion product otoacoustic emissions consistent with outer hair
    cell dysfunction.
  phenotype_term:
    preferred_term: Sensorineural hearing impairment
    term:
      id: HP:0000407
      label: Sensorineural hearing impairment
  evidence:
  - reference: PMID:38254937
    reference_title: "KMT2D Deficiency Causes Sensorineural Hearing Loss in Mice and Humans."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      individuals have both sensorineural and conductive hearing loss, with
      the average age of presentation being 7 years.
    explanation: Documents sensorineural hearing loss as a recurring component of the Kabuki hearing phenotype.
  - reference: PMID:38254937
    reference_title: "KMT2D Deficiency Causes Sensorineural Hearing Loss in Mice and Humans."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      The KS1 mice also display diminished distortion product otoacoustic
      emission levels, which suggests outer hair cell dysfunction.
    explanation: Supports a cell-intrinsic outer hair cell mechanism for sensorineural hearing loss.
- name: Seizures
  category: Neurological
  frequency: OCCASIONAL
  description: >-
    Seizures occur in a minority of patients with Kabuki syndrome,
    reported at 26.1% in a Taiwanese cohort.
  phenotype_term:
    preferred_term: Seizure
    term:
      id: HP:0001250
      label: Seizure
  evidence:
  - reference: PMID:39202303
    reference_title: "Genetic and Phenotypic Spectrum of KMT2D Variants in Taiwanese Case Series of Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Other abnormalities included hearing loss (39.1%), seizures (26.1%),
      cleft palate (26.1%), and renal anomalies (21.7%).
    explanation: Quantifies seizure prevalence at 26.1% in a 23-patient Taiwanese KS cohort.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0001250 | Seizure | Occasional (29-5%)"
    explanation: Orphanet classifies seizures as occasional in Kabuki syndrome.
- name: Speech Delay
  category: Neurological
  frequency: VERY_FREQUENT
  description: >-
    Delayed speech and language development is a near-universal feature of
    Kabuki syndrome, reported in 78.3% of a 23-patient Taiwanese cohort.
    Reflects both intellectual disability and the structural/oromotor
    contributions of cleft palate, hearing loss, and hypotonia.
  phenotype_term:
    preferred_term: Speech delay
    term:
      id: HP:0000750
      label: Delayed speech and language development
  evidence:
  - reference: PMID:39202303
    reference_title: "Genetic and Phenotypic Spectrum of KMT2D Variants in Taiwanese Case Series of Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The most common clinical characteristics included distinct facial
      features (100%), intellectual disability (100%), developmental delay
      (95.7%), speech delay (78.3%), hypotonia (69.6%), congenital heart
      abnormalities (69.6%), and recurrent infections (65.2%).
    explanation: Quantifies speech delay at 78.3% in a 23-patient Taiwanese KS cohort.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0012758 | Neurodevelopmental delay | Very frequent (99-80%)"
    explanation: Orphanet classifies neurodevelopmental delay (encompassing speech delay) as very frequent in Kabuki syndrome.
- name: Feeding Difficulties
  category: Gastrointestinal
  frequency: FREQUENT
  description: >-
    Feeding difficulties are common in infancy and early childhood, often
    requiring thickened feeds and positioning strategies.
  phenotype_term:
    preferred_term: Feeding difficulties
    term:
      id: HP:0011968
      label: Feeding difficulties
  evidence:
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0011968 | Feeding difficulties | Frequent (79-30%)"
    explanation: Orphanet classifies feeding difficulties as frequent in Kabuki syndrome.
- name: Failure to Thrive
  category: Growth
  frequency: FREQUENT
  description: >-
    Failure to thrive in infancy is common in Kabuki syndrome,
    often preceding a shift to overweight or obesity in later childhood.
  phenotype_term:
    preferred_term: Failure to thrive
    term:
      id: HP:0001508
      label: Failure to thrive
  evidence:
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0001508 | Failure to thrive | Frequent (79-30%)"
    explanation: Orphanet classifies failure to thrive as frequent in Kabuki syndrome.
  - reference: PMID:15108197
    reference_title: "Growth, behavior, and clinical findings in 27 patients with Kabuki (Niikawa-Kuroki) syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Six of these eight patients had failure to thrive in infancy.
    explanation: Documents failure to thrive in infancy in over half of patients studied.
- name: Joint Hypermobility
  category: Musculoskeletal
  frequency: FREQUENT
  description: >-
    Joint hypermobility and laxity are frequently observed, contributing
    to motor delays and joint dislocations.
  phenotype_term:
    preferred_term: Joint hypermobility
    term:
      id: HP:0001382
      label: Joint hypermobility
  evidence:
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0001382 | Joint hypermobility | Frequent (79-30%)"
    explanation: Orphanet classifies joint hypermobility as frequent in Kabuki syndrome.
- name: Strabismus
  category: Ophthalmological
  frequency: FREQUENT
  description: >-
    Strabismus is a frequent ophthalmological finding in Kabuki syndrome.
  phenotype_term:
    preferred_term: Strabismus
    term:
      id: HP:0000486
      label: Strabismus
  evidence:
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0000486 | Strabismus | Frequent (79-30%)"
    explanation: Orphanet classifies strabismus as frequent in Kabuki syndrome.
  - reference: PMID:21882399
    reference_title: "Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      ptosis and strabismus, and widely spaced teeth and hypodontia.
    explanation: GeneReviews lists strabismus among recognized Kabuki syndrome findings.
- name: Ptosis
  category: Ophthalmological
  frequency: FREQUENT
  description: >-
    Ptosis is a frequently reported ophthalmological feature of Kabuki
    syndrome.
  phenotype_term:
    preferred_term: Ptosis
    term:
      id: HP:0000508
      label: Ptosis
  evidence:
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0000508 | Ptosis | Frequent (79-30%)"
    explanation: Orphanet classifies ptosis as frequent in Kabuki syndrome.
  - reference: PMID:21882399
    reference_title: "Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      ptosis and strabismus, and widely spaced teeth and hypodontia.
    explanation: GeneReviews lists ptosis among recognized Kabuki syndrome findings.
- name: Premature Thelarche
  category: Endocrine
  frequency: FREQUENT
  description: >-
    Premature breast development (isolated premature thelarche) is
    frequently reported in female infants and young girls with Kabuki
    syndrome.
  phenotype_term:
    preferred_term: Premature thelarche
    term:
      id: HP:0010314
      label: Premature thelarche
  evidence:
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0010314 | Premature thelarche | Frequent (79-30%)"
    explanation: Orphanet classifies premature thelarche as frequent in Kabuki syndrome.
  - reference: PMID:3067577
    reference_title: "Kabuki make-up (Niikawa-Kuroki) syndrome: a study of 62 patients."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      early breast development in infant girls (23%)
    explanation: Niikawa et al. documented premature thelarche in 23% of the original 62-patient cohort.
- name: Cryptorchidism
  category: Genitourinary
  frequency: OCCASIONAL
  description: >-
    Cryptorchidism is an occasional genitourinary finding in males
    with Kabuki syndrome.
  phenotype_term:
    preferred_term: Cryptorchidism
    term:
      id: HP:0000028
      label: Cryptorchidism
  evidence:
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0000028 | Cryptorchidism | Occasional (29-5%)"
    explanation: Orphanet classifies cryptorchidism as occasional in Kabuki syndrome.
- name: Obesity
  category: Growth
  frequency: OCCASIONAL
  description: >-
    Obesity may develop in later childhood or adolescence, often
    following infantile failure to thrive.
  phenotype_term:
    preferred_term: Obesity
    term:
      id: HP:0001513
      label: Obesity
  evidence:
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0001513 | Obesity | Occasional (29-5%)"
    explanation: Orphanet classifies obesity as occasional in Kabuki syndrome.
  - reference: PMID:15108197
    reference_title: "Growth, behavior, and clinical findings in 27 patients with Kabuki (Niikawa-Kuroki) syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Eight of 14 patients over the age of 5 years were overweight or obese.
    explanation: Documents the characteristic growth trajectory from infantile failure to thrive to later obesity.
- name: Abnormal Dental Morphology
  category: Craniofacial
  frequency: FREQUENT
  description: >-
    Dental anomalies including hypodontia, widely spaced teeth, and
    microdontia are frequent findings in Kabuki syndrome.
  phenotype_term:
    preferred_term: Abnormal dental morphology
    term:
      id: HP:0006482
      label: Abnormal dental morphology
  evidence:
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0006482 | Abnormal dental morphology | Frequent (79-30%)"
    explanation: Orphanet classifies abnormal dental morphology as frequent in Kabuki syndrome.
  - reference: PMID:21882399
    reference_title: "Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      widely spaced teeth and hypodontia.
    explanation: GeneReviews lists dental anomalies among recognized Kabuki syndrome findings.
- name: Gastroesophageal Reflux
  category: Gastrointestinal
  frequency: OCCASIONAL
  description: >-
    Gastroesophageal reflux is an occasional finding, particularly in
    infancy, often concurrent with feeding difficulties.
  phenotype_term:
    preferred_term: Gastroesophageal reflux
    term:
      id: HP:0002020
      label: Gastroesophageal reflux
  evidence:
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "HP:0002020 | Gastroesophageal reflux | Occasional (29-5%)"
    explanation: Orphanet classifies gastroesophageal reflux as occasional in Kabuki syndrome.
  - reference: PMID:21882399
    reference_title: "Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Thickened feedings and positioning after meals to treat gastroesophageal reflux
    explanation: GeneReviews recommends management of gastroesophageal reflux as part of Kabuki syndrome care.
prevalence:
- population: Worldwide
  measure_type: BIRTH_PREVALENCE
  prevalence_class: BAND_1_9_PER_100000
  rate_per_100000: 3.0
  percentage: 0.003
  notes: >-
    Estimated prevalence of approximately 1 in 32,000 live births. This is
    likely an underestimate as milder cases may go undiagnosed.
  evidence:
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "1-9 / 100 000 | Japan | Prevalence at birth | PMID:3067577"
    explanation: Orphanet cites prevalence at birth of 1-9 per 100,000 in Japan.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "1-9 / 100 000 | Australia | Prevalence at birth | PMID:15108197"
    explanation: Orphanet cites prevalence at birth of 1-9 per 100,000 in Australia.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "1-9 / 100 000 | Europe | Point prevalence | EXPERT,PMID:21882399"
    explanation: Orphanet cites a European point prevalence of 1-9 per 100,000.
  - reference: PMID:3067577
    reference_title: "Kabuki make-up (Niikawa-Kuroki) syndrome: a study of 62 patients."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      It was estimated that its prevalence in Japanese newborn infants is
      1/32,000.
    explanation: Original Japanese epidemiological estimate of 1 in 32,000 live births.
  - reference: PMID:15108197
    reference_title: "Growth, behavior, and clinical findings in 27 patients with Kabuki (Niikawa-Kuroki) syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The minimum birth prevalence was calculated at 1 in 86,000.
    explanation: Australian/New Zealand estimate of minimum birth prevalence at 1 in 86,000.
datasets:
- accession: geo:GSE149688
  title: The KMT2D Kabuki syndrome histone methylase controls neural crest cell differentiation and facial morphology
  description: >-
    RNA-seq from E14.25 wild-type and Kmt2d neural crest cell knockout mouse
    palatal shelves, supporting analysis of KMT2D-dependent craniofacial
    developmental gene expression.
  organism:
    preferred_term: mouse
    term:
      id: NCBITaxon:10090
      label: Mus musculus
  data_type: BULK_RNA_SEQ
  conditions:
  - E14.25 wild-type palatal shelves
  - E14.25 Kmt2d neural crest cell knockout palatal shelves
  publication: PMID:32541010
  notes: >-
    GEO series linked to the KMT2D neural crest and palatal shelf model used to
    study craniofacial features of Kabuki syndrome.
  evidence:
  - reference: PMID:32541010
    reference_title: "The KMT2D Kabuki syndrome histone methylase controls neural crest cell differentiation and facial morphology."
    supports: PARTIAL
    evidence_source: MODEL_ORGANISM
    snippet: >-
      We mutated KMT2D in neural crest cells (NCCs) to study cellular and
      molecular functions in craniofacial development with respect to UTX.
    explanation: >-
      Supports the mouse neural crest experimental context for this GEO RNA-seq
      dataset; the GEO accession identifies the deposited series.
treatments:
- name: HDAC Inhibitor Therapy (Experimental)
  description: >-
    Histone deacetylase inhibitors such as AR-42 have shown preclinical
    promise in rescuing structural and functional brain deficits in Kmt2d
    haploinsufficient mouse models. By promoting histone acetylation, HDAC
    inhibitors can partially compensate for the reduced H3K4 methylation
    caused by KMT2D deficiency.
  treatment_term:
    preferred_term: HDAC inhibitor therapy
    term:
      id: NCIT:C15986
      label: Pharmacotherapy
  target_mechanisms:
  - target: Postnatal neurogenesis deficit
    treatment_effect: RESTORES
    description: HDAC inhibition increases histone acetylation, partially compensating for reduced H3K4 methylation and rescuing adult neurogenesis.
    evidence:
    - reference: PMID:25273096
      reference_title: "Histone deacetylase inhibition rescues structural and functional brain deficits in a mouse model of Kabuki syndrome."
      supports: SUPPORT
      evidence_source: MODEL_ORGANISM
      snippet: >-
        These abnormalities improved upon postnatal treatment with AR-42.
      explanation: Demonstrates rescue of neurogenesis-related abnormalities by HDAC inhibition.
  evidence:
  - reference: PMID:25273096
    reference_title: "Histone deacetylase inhibition rescues structural and functional brain deficits in a mouse model of Kabuki syndrome."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      These abnormalities improved upon postnatal treatment with AR-42. Our
      work suggests that a reversible deficiency in postnatal neurogenesis
      underlies intellectual disability in Kabuki syndrome.
    explanation: >-
      Demonstrates that HDAC inhibitor treatment can rescue neurogenesis and
      memory deficits in a Kabuki syndrome mouse model.
- name: Growth Hormone Therapy
  description: >-
    Growth hormone replacement may be considered for patients with
    documented growth hormone deficiency contributing to postnatal growth
    retardation.
  treatment_term:
    preferred_term: Growth hormone replacement therapy
    term:
      id: MAXO:0000780
      label: human growth hormone replacement therapy
  target_mechanisms:
  - target: Chondrocyte differentiation defect
    treatment_effect: BYPASSES
    description: Exogenous GH/IGF-1 stimulation drives chondrocyte proliferation at the growth plate, partially bypassing the KMT2D/KDM6A-dependent enhancer defect in chondrocyte differentiation.
  notes: >-
    Short stature is a core feature of KS. Growth hormone therapy has been
    used in patients with documented GH deficiency, though abstract-level
    evidence for efficacy is limited.
  evidence:
  - reference: PMID:28793284
    reference_title: "Growth Hormone Therapy in Children with Kabuki Syndrome: 1-year Treatment Results."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      All participants experienced catch-up growth during the year of rhGH
      treatment, but without an influence on body proportions.
    explanation: Supports recombinant human growth hormone as a treatment that can improve linear growth in children with Kabuki syndrome.
- name: Speech Therapy
  description: >-
    Speech and language therapy is an important component of developmental
    support given the high prevalence of speech delay and intellectual
    disability.
  treatment_term:
    preferred_term: Speech therapy
    term:
      id: MAXO:0000930
      label: speech therapy
  evidence:
  - reference: PMID:15523636
    reference_title: "Developmental outcome in Kabuki syndrome."
    supports: PARTIAL
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Based on these patients and a review of the literature, in the absence
      of major structural brain anomalies, the average intelligence quotient
      (IQ) in patients with this condition fall within the mild mental
      retardation range, however, specific developmental outcomes are widely
      variable, ranging from severe MR to normal intelligence.
    explanation: >-
      Supports significant developmental variability warranting early
      intervention; speech therapy specifically is not discussed in this paper.
- name: Cardiac Surgery
  description: >-
    Surgical repair of congenital heart defects including septal defects,
    coarctation of the aorta, and other structural cardiac anomalies.
  treatment_term:
    preferred_term: Cardiac surgery
    term:
      id: MAXO:0025001
      label: surgical procedure on cardiovascular system
  target_mechanisms:
  - target: Cardiomyocyte gene-expression dysregulation
    treatment_effect: BYPASSES
    description: Surgical repair corrects the structural consequence of cardiomyocyte and neural crest developmental defects without addressing the upstream chromatin mechanism.
  - target: Neural crest developmental dysregulation
    treatment_effect: BYPASSES
    description: Surgery addresses outflow-tract and septal defects produced by cardiac neural crest dysfunction.
  evidence:
  - reference: PMID:11343317
    reference_title: "Congenital heart defects in Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      CHD was diagnosed in 35 (58%) of our patients. Aortic coarctation (COA)
      (23%), atrial septal defect (ASD) (20%), and ventricular septal defect
      (VSD) (17%) were the most frequent CHDs
    explanation: >-
      High frequency of congenital heart defects necessitates surgical
      evaluation and repair.
- name: Immunoglobulin Replacement
  description: >-
    Intravenous or subcutaneous immunoglobulin replacement therapy for
    patients with severe hypogammaglobulinemia and recurrent infections.
  treatment_term:
    preferred_term: Immunoglobulin replacement therapy
    term:
      id: MAXO:0001480
      label: immunoglobulin infusion therapy
    therapeutic_agent:
    - preferred_term: therapeutic immune globulin
      term:
        id: NCIT:C2701
        label: Therapeutic Immune Globulin
  target_mechanisms:
  - target: B-cell terminal differentiation defect
    treatment_effect: BYPASSES
    description: Exogenous immunoglobulin compensates for impaired endogenous antibody production from defective terminal B-cell differentiation.
  evidence:
  - reference: PMID:15887282
    reference_title: "Immune abnormalities are a frequent manifestation of Kabuki syndrome."
    supports: PARTIAL
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Due to this increased susceptibility to infection, children with KS
      should have immunologic evaluations at the time of diagnosis in order to
      reduce preventable morbidity and mortality.
    explanation: >-
      Supports the clinical need for immunologic work-up but does not
      explicitly recommend immunoglobulin replacement therapy.
- name: KDM1A Inhibitor (Experimental)
  description: >-
    Lysine-specific demethylase 1A (KDM1A/LSD1) normally removes H3K4 methyl
    marks added by KMT2D. Pharmacologic KDM1A inhibition (e.g., TAK-418)
    rebalances H3K4 methylation, restores postnatal neurogenesis, and
    rescues hippocampal memory in Kmt2d-haploinsufficient mice. Targets the
    upstream chromatin defect more directly than HDAC inhibition.
  treatment_term:
    preferred_term: Pharmacotherapy
    term:
      id: NCIT:C15986
      label: Pharmacotherapy
  target_mechanisms:
  - target: Postnatal neurogenesis deficit
    treatment_effect: RESTORES
    description: KDM1A inhibition restores the H3K4me-balance lost in KMT2D haploinsufficiency, rescuing adult hippocampal neurogenesis.
    evidence:
    - reference: PMID:33738331
      reference_title: "Inhibition of KDM1A activity restores adult neurogenesis and improves hippocampal memory in a mouse model of Kabuki syndrome."
      supports: SUPPORT
      evidence_source: MODEL_ORGANISM
      snippet: >-
        After 2 weeks of TAK-418, Kmt2d +/βGeo mice demonstrated
        normalization of hippocampal memory defects.
      explanation: Direct mechanism-targeted rescue of the neurogenesis-memory phenotype in a Kabuki mouse model.
  evidence:
  - reference: PMID:33738331
    reference_title: "Inhibition of KDM1A activity restores adult neurogenesis and improves hippocampal memory in a mouse model of Kabuki syndrome."
    supports: SUPPORT
    evidence_source: MODEL_ORGANISM
    snippet: >-
      our data suggest that KDM1A inhibition is a plausible treatment
      strategy for KS
    explanation: Establishes KDM1A inhibition as a rational chromatin-targeted treatment strategy for Kabuki syndrome.
- name: Modified Atkins Diet (Investigational)
  description: >-
    Ketogenic/Modified Atkins dietary intervention is being investigated as
    a chromatin-modulating therapy in Kabuki syndrome. Multi-omics data
    show partial reversal of ribosomal protein dysregulation in a treated
    KMT2D KS patient. A completed 12-week adult phase-1 trial (NCT04722315)
    evaluated cognitive and methylation outcomes.
  treatment_term:
    preferred_term: dietary intervention
    term:
      id: MAXO:0000088
      label: dietary intervention
  target_mechanisms:
  - target: Chromatin dysregulation from KMT2D and KDM6A haploinsufficiency
    treatment_effect: MODULATES
    description: Ketogenic state alters histone acetylation and chromatin accessibility, partially compensating for KMT2D-driven enhancer dysregulation.
    evidence:
    - reference: PMID:38768529
      reference_title: "Ketogenic diet modifies ribosomal protein dysregulation in KMT2D Kabuki syndrome."
      supports: PARTIAL
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        A 12-year-old boy with KS, suffering from recurrent episodes of
        cognitive decline, exhibited improved cognitive function and
        neuropsychological assessment performance after 12 months on the
        ketogenic diet, with concomitant improvement in transcriptomic
        ribosomal protein dysregulation.
      explanation: Demonstrates that ketogenic diet can modulate KMT2D-related ribosomal protein dysregulation with concurrent clinical improvement, supporting chromatin-modulating treatment targeting.
- name: Diazoxide
  description: >-
    First-line pharmacotherapy for hyperinsulinemic hypoglycemia in Kabuki
    syndrome. Activates pancreatic beta-cell K-ATP channels to suppress
    insulin secretion. Approximately 92% of children with KS-associated
    hyperinsulinism respond to a diazoxide trial.
  treatment_term:
    preferred_term: Pharmacotherapy
    term:
      id: NCIT:C15986
      label: Pharmacotherapy
    therapeutic_agent:
    - preferred_term: diazoxide
      term:
        id: CHEBI:4495
        label: diazoxide
  target_mechanisms:
  - target: Pancreatic beta-cell dysfunction
    treatment_effect: INHIBITS
    description: Diazoxide opens beta-cell K-ATP channels, suppressing dysregulated insulin secretion in KS-associated hyperinsulinism.
    evidence:
    - reference: PMID:38859884
      reference_title: "Clinical and Molecular Characterization of Hyperinsulinism in Kabuki Syndrome."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Diazoxide trial was conducted in 25 children, 92% of whom were
        responsive.
      explanation: Demonstrates high clinical response rate to diazoxide for KS-associated hyperinsulinism.
  evidence:
  - reference: PMID:38859884
    reference_title: "Clinical and Molecular Characterization of Hyperinsulinism in Kabuki Syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      HI treatment was discontinued in 46% of the cohort at median age 2.8
      years
    explanation: Documents that medical management of KS-associated hyperinsulinism, primarily with diazoxide, frequently allows treatment discontinuation in early childhood.
clinical_trials:
- name: NCT04722315
  phase: PHASE_I
  status: COMPLETED
  description: >-
    Single-group early phase 1 trial evaluating a 12-week Modified Atkins
    Diet in adults with genetically confirmed Kabuki syndrome, with
    cognitive/visuospatial/memory outcomes plus serial genome-wide DNA
    methylation measurements.
  target_phenotypes:
  - preferred_term: Intellectual disability
    term:
      id: HP:0001249
      label: Intellectual disability
  evidence:
  - reference: clinicaltrials:NCT04722315
    reference_title: "Pilot Clinical Trial of Modified Atkins Diet for Kabuki Syndrome"
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      This study aims to examine a small number of adults with Kabuki
      syndrome before and after 12 weeks on a modified Atkins diet to
      determine if there is any cognitive improvement and if the diet can
      be tolerated.
    explanation: Documents the trial's design and primary cognitive endpoint, anchoring the Modified Atkins Diet treatment in completed human evidence.
  - reference: clinicaltrials:NCT04722315
    reference_title: "Pilot Clinical Trial of Modified Atkins Diet for Kabuki Syndrome"
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Modified Atkins diet is safer and easier tolerated than full
      ketogenic diet and still has the histone deacetylase inhibition
      believed to be responsible for the cognitive improvement.
    explanation: Provides the mechanistic rationale (HDAC inhibition via ketones) for testing Modified Atkins Diet in Kabuki syndrome.
genetic:
- name: KMT2D
  gene_term:
    preferred_term: KMT2D
    term:
      id: hgnc:7133
      label: KMT2D
  association: Causative
  notes: >-
    KMT2D is the major Kabuki syndrome gene. Many pathogenic variants are
    truncating and reduce KMT2D dosage through nonsense-mediated decay,
    consistent with haploinsufficiency.
  evidence:
  - reference: PMID:20711175
    reference_title: "Exome sequencing identifies MLL2 mutations as a cause of Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Our results strongly suggest that mutations in MLL2 are a major cause of
      Kabuki syndrome.
    explanation: Establishes KMT2D as the major causative gene.
  - reference: PMID:24633898
    reference_title: "Molecular analysis, pathogenic mechanisms, and readthrough therapy on a large cohort of Kabuki syndrome patients."
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      We found that a number of KMT2D truncating mutations result in mRNA
      degradation through the nonsense-mediated mRNA decay, contributing to
      protein haploinsufficiency.
    explanation: Supports KMT2D haploinsufficiency as the dominant genetic mechanism.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "KMT2D | lysine methyltransferase 2D | hgnc:7133 | Disease-causing germline mutation(s) in"
    explanation: Orphanet lists KMT2D as a disease-causing gene for Kabuki syndrome.
- name: KDM6A
  gene_term:
    preferred_term: KDM6A
    term:
      id: hgnc:12637
      label: KDM6A
  association: Causative
  notes: >-
    KDM6A is a less common Kabuki syndrome gene that encodes a histone
    demethylase functionally linked to KMT2D.
  evidence:
  - reference: PMID:22197486
    reference_title: "Deletion of KDM6A, a histone demethylase interacting with MLL2, in three patients with Kabuki syndrome."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Here, we describe three KS individuals with de novo partial or complete
      deletions of an X chromosome gene, KDM6A, that encodes a histone
      demethylase that interacts with MLL2.
    explanation: Establishes KDM6A as a causative Kabuki syndrome gene.
  - reference: ORPHA:2322
    reference_title: "Kabuki syndrome (Orphanet structured-database record)"
    supports: SUPPORT
    snippet: "KDM6A | lysine demethylase 6A | hgnc:12637 | Disease-causing germline mutation(s) in"
    explanation: Orphanet lists KDM6A as a disease-causing gene for Kabuki syndrome.
📚

References & Deep Research

Deep Research

1
Falcon
Kabuki Syndrome (MONDO:0016512) — Disease Characteristics Research Report
Edison Scientific Literature 44 citations 2026-04-25T18:47:41.219641

Kabuki Syndrome (MONDO:0016512) — Disease Characteristics Research Report

1. Disease Information

1.1 Overview (definition and current understanding)

Kabuki syndrome (KS) is a rare, congenital, multisystem neurodevelopmental disorder characterized by a recognizable facial gestalt, developmental delay/intellectual disability, skeletal anomalies, dermatoglyphic anomalies (including persistent fetal fingertip pads), and postnatal growth deficiency, with additional frequent involvement of cardiac, renal, hearing, immune, endocrine, and gastrointestinal systems. (barry2022fromgenotypeto pages 1-2, dugan2021kabukisyndrome pages 1-3, boniel2021kabukisyndrome—clinicalreview pages 1-2)

Epidemiologic frequency (range reported in large review): estimated frequency ~1:32,000–1:86,000. (barry2022fromgenotypeto pages 1-2)

Evidence type: aggregated disease-level literature review synthesizing 152 publications and 1369 individuals. (barry2022fromgenotypeto pages 1-2, barry2022fromgenotypeto pages 2-4)

1.2 Key identifiers and synonyms

A structured list of identifiers available from the retrieved sources is provided here:

Identifier system Identifier/value Notes
MONDO MONDO:0016512 User-provided disease identifier for Kabuki syndrome. Not independently verified in retrieved evidence.
OMIM Kabuki syndrome 1 (KS1): 147920 KMT2D-related Kabuki syndrome; autosomal dominant in retrieved reviews/management sources (dugan2021kabukisyndrome pages 1-3, boniel2021kabukisyndrome—clinicalreview pages 1-2)
OMIM Kabuki syndrome 2 (KS2): 300867 KDM6A-related Kabuki syndrome; X-linked in retrieved reviews/management sources (dugan2021kabukisyndrome pages 1-3, boniel2021kabukisyndrome—clinicalreview pages 1-2)
OMIM / gene KMT2D: 602113 Major causal gene for KS1; cited in recent mechanistic and clinical reviews (golden2023molecularinsightsof pages 1-3, golden2023molecularinsightsof pages 9-11)
OMIM / gene KDM6A: 300128 Causal gene for KS2; X-linked histone demethylase noted in retrieved reviews and KS2 cohort study (golden2023molecularinsightsof pages 1-3, wang2024sexspecificdifferencein pages 2-4)
Disease name Kabuki syndrome Preferred disease name across retrieved sources (barry2022fromgenotypeto pages 1-2, adam2019kabukisyndromeinternational pages 1-2)
Synonym Kabuki make-up syndrome Explicit synonym in management/review sources (dugan2021kabukisyndrome pages 1-3, boniel2021kabukisyndrome—clinicalreview pages 8-9)
Synonym Niikawa–Kuroki syndrome Historical synonym in review sources (barry2022fromgenotypeto pages 1-2, dugan2021kabukisyndrome pages 1-3)
Orphanet Not in retrieved sources No Orphanet identifier was present in the gathered evidence.
MeSH Not in retrieved sources No MeSH identifier was present in the gathered evidence.
ICD-10 Not in retrieved sources No ICD-10 code was present in the gathered evidence.
ICD-11 Not in retrieved sources No ICD-11 code was present in the gathered evidence.

Table: This table summarizes the key disease names and identifiers for Kabuki syndrome that were supported by retrieved evidence, including KS1/KS2 OMIM entries and common synonyms. Fields not found in the evidence are explicitly marked to avoid overclaiming.

Synonyms supported by retrieved sources include Kabuki make-up syndrome and Niikawa–Kuroki syndrome. (barry2022fromgenotypeto pages 1-2, dugan2021kabukisyndrome pages 1-3, boniel2021kabukisyndrome—clinicalreview pages 8-9)

Note on missing identifiers: Orphanet IDs, MeSH IDs, and ICD-10/ICD-11 codes were not present in the retrieved documents available to this run; therefore they are not asserted here. (artifact-00)

1.3 Consensus diagnostic framing (expert consensus)

The international consensus diagnostic criteria emphasize a recognisable clinical pattern plus molecular confirmation when available.

Direct abstract quote (consensus paper):The authors propose that a definitive diagnosis can be made in an individual of any age with a history of infantile hypotonia, developmental delay and/or intellectual disability, and one or both of the following major criteria: (1) a pathogenic or likely pathogenic variant in KMT2D or KDM6A; and (2) typical dysmorphic features…” (Adam et al., 2019, Journal of Medical Genetics, published online 2019; DOI URL: https://doi.org/10.1136/jmedgenet-2018-105625). (adam2019kabukisyndromeinternational pages 1-2)

2. Etiology

2.1 Primary causal factors (genetic)

KS is primarily a Mendelian disorder caused by pathogenic variants in chromatin regulators: - KMT2D (KS1; autosomal dominant): heterozygous dominant loss-of-function variants are the most common cause (often de novo). (barry2022fromgenotypeto pages 1-2, jung2023characterizingthemolecular pages 1-5, golden2023molecularinsightsof pages 1-3) - KDM6A (KS2; X-linked): heterozygous (female) or hemizygous (male) variants cause KS2. (barry2022fromgenotypeto pages 1-2, dugan2021kabukisyndrome pages 1-3, wang2024sexspecificdifferencein pages 2-4)

Mechanistically, KMT2D is an H3K4 methyltransferase, and KDM6A is an H3K27 demethylase; both are key components of enhancer/promoter chromatin regulation during development. (golden2023molecularinsightsof pages 1-3, boniel2021kabukisyndrome—clinicalreview pages 2-3)

2.2 Risk factors / protective factors / gene–environment interactions

For KS (a monogenic syndrome), the predominant “risk factor” is carrying a pathogenic germline variant in KMT2D or KDM6A; additional environmental risk and protective factors are not well-defined in the retrieved sources. (barry2022fromgenotypeto pages 1-2, dugan2021kabukisyndrome pages 1-3)

A plausible gene–environment interaction discussed in recent intervention work is that metabolic state (ketosis via dietary intervention) may modulate downstream molecular phenotypes (e.g., ribosomal/protein-translation pathways) in KMT2D-related KS. (tsang2024ketogenicdietmodifies pages 8-10, tsang2024ketogenicdietmodifies pages 2-3)

3. Phenotypes (with HPO suggestions)

3.1 Core phenotypic domains

Across large reviews and clinical management sources, commonly described domains include: - Craniofacial gestalt (long palpebral fissures with lower-lid eversion; arched/broad eyebrows with lateral sparseness; depressed nasal tip/short columella; prominent/cupped ears) (adam2019kabukisyndromeinternational pages 1-2, dugan2021kabukisyndrome pages 1-3) - Neurodevelopmental phenotype (developmental delay; intellectual disability; hypotonia) (barry2022fromgenotypeto pages 1-2, dugan2021kabukisyndrome pages 1-3) - Skeletal anomalies and persistent fingertip pads (barry2022fromgenotypeto pages 1-2, adam2019kabukisyndromeinternational pages 1-2) - Postnatal growth deficiency/short stature (barry2022fromgenotypeto pages 1-2, boniel2021kabukisyndrome—clinicalreview pages 1-2) - Congenital heart disease (barry2022fromgenotypeto pages 1-2, lee2024geneticandphenotypic pages 1-2) - Immune dysfunction (recurrent infections, hypogammaglobulinemia; autoimmunity in a subset) (margot2020immunopathologicalmanifestationsin pages 1-2) - Hearing loss (conductive and sensorineural components) (kalinousky2023kmt2ddeficiencycauses pages 5-7)

3.2 Recent cohort statistics (2024 Taiwanese case series)

A 2024 Taiwanese case series (n=23) provides concrete phenotype frequencies (primarily KMT2D): - Distinct facial features: 100% - Intellectual disability: 100% - Developmental delay: 95.7% - Speech delay: 78.3% - Hypotonia: 69.6% - Congenital heart abnormalities: 69.6% - Recurrent infections: 65.2% - Hearing loss: 39.1% - Seizures: 26.1% - Cleft palate: 26.1% - Renal anomalies: 21.7% (Lee et al., 2024, Diagnostics, Aug 2024; DOI URL: https://doi.org/10.3390/diagnostics14161815). (lee2024geneticandphenotypic pages 1-2)

Cardiac lesion distribution within CHD subset (n=16): ASD 37.5% (6/16), VSD 18.8% (3/16), aortic coarctation 18.8% (3/16). (lee2024geneticandphenotypic pages 6-7, lee2024geneticandphenotypic pages 4-6)

3.3 Hearing phenotype (recent 2023 human+mouse study)

A 2023 KS1 study (KMT2D; n=21 individuals) reported: - Current hearing loss in 71.43% (15/21) - Female skew in that cohort: all 12 females reported hearing loss vs 3/9 (33.33%) males - Among those with hearing loss and reported type (n=10): 6 sensorineural, 1 conductive, 3 mixed - Structural ear abnormalities in 19.05% (4/21) (Kalinousky et al., 2023, Genes, Dec 2023; DOI URL: https://doi.org/10.3390/genes15010048). (kalinousky2023kmt2ddeficiencycauses pages 5-7)

3.4 Immune/autoimmune phenotype statistics (registry)

A registry study (n=177; molecularly confirmed KMT2D/KDM6A) quantified: - Susceptibility to infections: 44.1% (78/177) - Hypogammaglobulinemia: 58.2% (46/79 tested) - Autoimmune disease overall: 13.6% (24/177); in adults: 25.6% (11/43) - Immune thrombocytopenic purpura: 7.3% (13/177); autoimmune hemolytic anemia: 4.0% (7/177) (Margot et al., 2020, Genetics in Medicine, Jan 2020; DOI URL: https://doi.org/10.1038/s41436-019-0623-x). (margot2020immunopathologicalmanifestationsin pages 1-2)

3.5 Quality of life / humanistic burden

Caregiver/adolescent report studies indicate substantial multidimensional burden; while this run did not extract instrument-level statistics (e.g., EQ-5D), a qualitative study reports “substantial negative effects on physical, mental, emotional, and social aspects of health-related quality of life.” (barry2022fromgenotypeto pages 1-2)

3.6 HPO term suggestions (non-exhaustive; ontology mapping suggestions)

(These are suggested mappings for knowledge-base structuring; the retrieved sources support the clinical concepts but do not enumerate HPO IDs.) - Facial gestalt: Long palpebral fissures; Everted lower eyelids; Arched eyebrows; Large/protruding ears - Neurodevelopment: Global developmental delay; Intellectual disability; Hypotonia; Speech delay - Growth: Postnatal growth retardation; Short stature - Cardiac: Congenital heart defect; Atrial septal defect; Ventricular septal defect; Coarctation of aorta - Immune: Recurrent infections; Hypogammaglobulinemia; Immune thrombocytopenia; Autoimmune hemolytic anemia - Hearing: Hearing impairment; Sensorineural hearing loss; Conductive hearing loss

4. Genetic / Molecular Information

4.1 Causal genes and inheritance

  • KMT2D (KS1): autosomal dominant; typically de novo but familial transmission occurs. (barry2022fromgenotypeto pages 1-2, dugan2021kabukisyndrome pages 1-3)
  • KDM6A (KS2): X-linked; sex-dependent expressivity is reported in recent KS2 cohorts. (wang2024sexspecificdifferencein pages 2-4, wang2024sexspecificdifferencein pages 5-6)

4.2 Variant classes and spectrum

Large aggregated reviews and recent KS1-focused review note broad variant classes including nonsense, frameshift, splice-site, indels, CNVs, and missense (some clustering near functional domains). (barry2022fromgenotypeto pages 2-4, golden2023molecularinsightsof pages 9-11)

Examples of quantitative variant spectrum reporting: - In the 2024 Taiwanese series (n=23), variant class frequencies were reported (patient-level): missense 26.1%, nonsense 21.7%, frameshift 17.4%. (lee2024geneticandphenotypic pages 1-2) - In the same study, among 16 unique KMT2D variants: nonsense 31.3%, missense 18.7%, frameshift 18.7%, deletions 18.7%, splicing 6.3%, insertion/deletion 6.3%. (lee2024geneticandphenotypic pages 2-4)

4.3 Epigenetic and transcriptional consequences (human evidence; 2023)

A 2023 study profiled PBMCs from 33 individuals with KMT2D-related KS and 36 controls, finding: - Distinct enhancer signatures in H3K4me1/H3K4me2 - Reduced promoter-distal enhancer signals at immune-related genes and overlap with ~31% of normal blood-cell super-enhancers - Increased enhancer signals near promoters of metabolic genes, with elevated transcription (Jung et al., 2023, Human Molecular Genetics, Oct 2023; DOI URL: https://doi.org/10.1101/2022.10.25.22280882). (jung2023characterizingthemolecular pages 1-5)

Direct abstract quote:…we profiled and characterized alterations in histone modification and gene transcription in peripheral blood mononuclear cells (PBMCs) from 33 patients with KMT2D mutations and 36 unaffected healthy controls.” (jung2023characterizingthemolecular pages 1-5)

4.4 Immune mechanism: integrin regulation in T cells (2024)

A 2024 mechanistic immunology study reports that KMT2D directly regulates leukocyte integrin loci (chromatin and expression), with functional impact on thymocyte migration/egress and peripheral T-cell composition.

Key mechanistic findings include: - Reduced expression of integrins (e.g., Itgal, Itgb7) at transcript and protein levels; H3K4me3 ChIP-PCR supports direct control (potter2024kmt2dregulatesactivation pages 1-2, potter2024kmt2dregulatesactivation pages 9-11) - Peripheral shifts in humans and mice, including reduced naïve/RTE and increased memory phenotypes (potter2024kmt2dregulatesactivation pages 14-16) (Potter et al., 2024, Frontiers in Immunology, May 2024; DOI URL: https://doi.org/10.3389/fimmu.2024.1341745). (potter2024kmt2dregulatesactivation pages 1-2)

4.5 Cell-type specificity of chromatin disruption (2024)

A 2024 Genome Research study in Kabuki mouse models indicates that chromatin accessibility abnormalities in neurons are largely distinct from those in peripheral B and T cells, with neuron-specific enrichment at CpG islands and aging-linked elements.

Direct abstract-level statement from retrieved text:…chromatin accessibility abnormalities in neurons are mostly distinct from those in B or T cells… Neurons, but not B or T cells, show preferential chromatin disruption at CpG islands and at regulatory elements linked to aging.” (Boukas et al., 2024, Genome Research, May 2024; DOI URL: https://doi.org/10.1101/gr.278416.123). (boukas2024neuronspecificchromatindisruption pages 1-2)

4.6 KS2 growth mechanism and convergence with KS1 (2024)

A 2024 KS2 mouse study (Kdm6a tm1d/+), focusing on endochondral ossification, found: - Decreased femur/tibia length; cortical and trabecular structural changes - Shorter growth plates, driven by reduced hypertrophic chondrocyte size and hypertrophic zone height - In vitro Kdm6a−/− cells showed premature/enhanced chondrocyte differentiation - RNA-seq showed convergent gene expression between Kdm6a−/− and Kmt2d−/− lines, suggesting shared downstream pathways (Gao et al., 2024, PLOS Genetics, Jun 2024; DOI URL: https://doi.org/10.1371/journal.pgen.1011310). (gao2024growthdeficiencyin pages 1-2, gao2024growthdeficiencyin pages 3-6)

5. Environmental Information

No specific, reproducible non-genetic environmental causal factors are described in the retrieved sources for Kabuki syndrome, consistent with its primary Mendelian etiology. (barry2022fromgenotypeto pages 1-2, dugan2021kabukisyndrome pages 1-3)

Dietary metabolic state (ketogenic/Modified Atkins) is an intervention rather than a causal environmental exposure and is covered under Treatment/Applications. (tsang2024ketogenicdietmodifies pages 8-10, NCT04722315 chunk 1)

6. Mechanism / Pathophysiology

6.1 Chromatin and histone-mark dysregulation (core causal chain)

Upstream trigger: germline loss-of-function (typically) variants in KMT2D (H3K4 methyltransferase) and/or KDM6A (H3K27 demethylase). (golden2023molecularinsightsof pages 1-3, boniel2021kabukisyndrome—clinicalreview pages 2-3)

Intermediate molecular consequence: altered enhancer/promoter chromatin state and transcriptional dysregulation, measurable in human immune cells as altered H3K4me1/H3K4me2 enhancer signatures with reduced enhancer activity at immune genes. (jung2023characterizingthemolecular pages 1-5)

Downstream cellular consequences: impaired maturation/function of immune cells (B- and T-cell defects; altered integrin programs and migration/egress), contributing to recurrent infections, hypogammaglobulinemia, and autoimmune manifestations. (margot2020immunopathologicalmanifestationsin pages 1-2, potter2024kmt2dregulatesactivation pages 14-16)

Clinical manifestations: multisystem developmental phenotype including neurodevelopmental delay, craniofacial anomalies, growth deficiency, heart defects, hearing loss, and immune disease. (barry2022fromgenotypeto pages 1-2, dugan2021kabukisyndrome pages 1-3, lee2024geneticandphenotypic pages 1-2, kalinousky2023kmt2ddeficiencycauses pages 5-7)

6.2 Immune pathway mechanism (integrin/MST1 axis)

In Kmt2d-deficient murine thymocytes, transcriptomics implicate integrin-linked migration programs (including Mst1 pathway-related genes such as Rap1a/Vasp and integrin genes) as dysregulated, aligning with abnormal T-cell maturation and peripheral distribution shifts. (potter2024kmt2dregulatesactivation pages 11-13, potter2024kmt2dregulatesactivation pages 14-16)

6.3 Neurodevelopment: tissue specificity and episignature caveat

The 2024 neuron-vs-blood chromatin accessibility study supports a model in which neurodevelopmental chromatin disruptions are not simply recapitulated by blood epigenomic patterns; neurons show preferential disruption at CpG islands and aging-linked regulatory elements. This helps explain why blood-derived episignatures can be diagnostically useful yet incomplete as mechanistic proxies for brain phenotypes. (boukas2024neuronspecificchromatindisruption pages 1-2)

6.4 Growth-plate biology and endochondral ossification

KS2 growth failure can arise from impaired hypertrophic chondrocyte enlargement (hypertrophic-zone shortening) and premature chondrogenic differentiation, with transcriptional convergence between KS1 and KS2 models supporting a shared downstream program affecting cartilage development. (gao2024growthdeficiencyin pages 1-2, gao2024growthdeficiencyin pages 3-6)

6.5 Suggested ontology terms (mechanism structuring; not exhaustively validated here)

  • GO biological process (suggested): chromatin organization; histone H3-K4 methylation; regulation of transcription by RNA polymerase II; T cell activation; leukocyte migration; endochondral ossification; chondrocyte differentiation.
  • Cell Ontology (suggested): B cell; T cell; CD4-positive T cell; CD8-positive T cell; thymocyte; natural killer cell; chondrocyte; hypertrophic chondrocyte; neuron.

7. Anatomical Structures Affected

7.1 Organ/system involvement (supported)

  • Central nervous system / neurodevelopment: developmental delay, intellectual disability, hypotonia. (barry2022fromgenotypeto pages 1-2, lee2024geneticandphenotypic pages 1-2)
  • Immune system: recurrent infections, hypogammaglobulinemia, autoimmune cytopenias. (margot2020immunopathologicalmanifestationsin pages 1-2)
  • Cardiovascular system: congenital heart abnormalities (e.g., ASD, VSD, coarctation). (lee2024geneticandphenotypic pages 1-2)
  • Auditory system: mixed conductive and sensorineural hearing loss. (kalinousky2023kmt2ddeficiencycauses pages 5-7)
  • Skeletal system / growth plates: growth deficiency and altered long-bone and growth-plate parameters (mouse models; mechanistic relevance). (gao2024growthdeficiencyin pages 3-6)

7.2 UBERON / GO-CC suggestions (not asserted as extracted identifiers)

  • UBERON (suggested): heart; cochlea; thymus; bone growth plate; hippocampus
  • GO cellular component (suggested): nucleus; chromatin; histone methyltransferase complex

8. Temporal Development

8.1 Typical onset

KS is congenital with early-life hypotonia and developmental delay emphasized in diagnostic criteria. (adam2019kabukisyndromeinternational pages 1-2)

8.2 Hearing temporal profile (example)

In a KS1 cohort, mean onset/presentation of hearing loss was reported as ~7 years, though some individuals had hearing loss at birth. (kalinousky2023kmt2ddeficiencycauses pages 5-7)

9. Inheritance and Population

9.1 Inheritance patterns

  • KS1 (KMT2D): autosomal dominant. (barry2022fromgenotypeto pages 1-2, dugan2021kabukisyndrome pages 1-3)
  • KS2 (KDM6A): X-linked; recent cohort work suggests sex-modified severity. (wang2024sexspecificdifferencein pages 2-4, wang2024sexspecificdifferencein pages 5-6)

9.2 Sex-specific severity in KS2 (2024 matched case–control)

In a KS2 cohort (n=12; males=5, females=7): - CHD: 5/5 (100%) males vs 1/7 (14.29%) females (P=0.015) - Moderate-to-severe intellectual disability (IQ<55): 4/4 (100%) assessed males vs 0/7 females (P=0.003) - Median IQ: 41 in males vs 69 in females (P=0.029) (Wang et al., 2024, BMC Pediatrics, Feb 2024; DOI URL: https://doi.org/10.1186/s12887-024-04562-z). (wang2024sexspecificdifferencein pages 5-6)

10. Diagnostics

10.1 Clinical criteria and confirmatory testing

The consensus criteria emphasize hypotonia and developmental delay/intellectual disability plus either a pathogenic/likely pathogenic variant in KMT2D/KDM6A and/or typical dysmorphism. (adam2019kabukisyndromeinternational pages 1-2)

10.2 Genetic testing (real-world implementation)

Recent reviews describe clinical implementation of WES/trio-WES and targeted sequencing to detect SNVs/indels and CNVs, with interpretive challenges including VUS and complex variant classes. (golden2023molecularinsightsof pages 9-11)

10.3 Epigenomic “episignature” as a diagnostic adjunct

DNA methylation episignatures are described as capable of identifying KS1 “regardless of variant class” in a review context, supporting clinical adoption of episignature testing when sequence findings are equivocal. (golden2023molecularinsightsof pages 9-11)

10.4 Differential diagnosis

This run did not retrieve differential-diagnosis tables or guideline-style differential lists; therefore no specific differentials are asserted here.

11. Outcome / Prognosis

The retrieved sources emphasize variable multisystem burden and the need for longitudinal adult natural history data, but did not provide robust survival or life-expectancy statistics in the extracted passages. (barry2022fromgenotypeto pages 1-2)

However, immune complications can be serious: immunopathological manifestations are described as “common and can be life-threatening,” supporting systematic screening and preventive management. (margot2020immunopathologicalmanifestationsin pages 1-2)

12. Treatment

12.1 Standard of care (current practice)

Clinical management is primarily supportive and multidisciplinary, including surveillance and treatment of congenital anomalies, developmental interventions, and management of immune dysfunction, feeding problems, endocrine issues, and seizures as they arise. (dugan2021kabukisyndrome pages 1-3, boniel2021kabukisyndrome—clinicalreview pages 1-2)

12.2 Dietary/metabolic interventions (recent developments and real-world implementation)

12.2.1 2024 multi-omics + Modified Atkins/ketogenic diet report (KMT2D)

A 2024 eBioMedicine study combined proteomics (KS n=4 vs controls n=4; significant protein changes at FDR<0.05) and scRNA-seq with a single-patient Modified Atkins/ketogenic-style dietary intervention.

Molecular findings included large-scale proteomic dysregulation and downregulation of ribosomal proteins/translation pathways in KS, with partial reversal of ribosomal gene dysregulation after 12 months of diet in the treated participant. (tsang2024ketogenicdietmodifies pages 8-10, tsang2024ketogenicdietmodifies pages 6-8)

Reported clinical signals in the treated child included elimination of “brain fog” episodes, improved neuropsychological testing domains (e.g., attention/impulse control), reduced school absenteeism (8.5→3 days/semester), and reduced antibiotic courses (8.5/year→3.7/year), though this is uncontrolled n=1 evidence. (tsang2024ketogenicdietmodifies pages 8-10)

Intervention details included initial carbohydrate restriction to 15 g/day, ketosis tracking with urinary ketones and serum beta-hydroxybutyrate (BOHB ~1.90–4.86 mmol/L). (tsang2024ketogenicdietmodifies pages 2-3)

Evidence type: n=1 intervention with supporting multi-omics; hypothesis-generating. (tsang2024ketogenicdietmodifies pages 8-10, tsang2024ketogenicdietmodifies pages 2-3)

12.2.2 ClinicalTrials.gov: Modified Atkins Diet trial (adults; completed)

A single-group early phase 1 trial evaluated 12-week Modified Atkins Diet in adults with genetically confirmed KS. - ClinicalTrials.gov ID: NCT04722315 - Status: Completed - Enrollment: 10 - Primary completion: 2024-01-26 - Results posted: 2025-05-13 - Outcome domains: cognitive/visuospatial/memory testing plus serial genome-wide DNA methylation measures. (NCT04722315 chunk 1)

URL: https://clinicaltrials.gov/study/NCT04722315 (NCT04722315 chunk 1)

Note: numeric outcome results were not extracted from the record text available in this run. (NCT04722315 chunk 1)

12.3 MAXO term suggestions (treatment action structuring)

  • Multidisciplinary care coordination
  • Genetic counseling
  • Developmental therapy (speech therapy; physical therapy; occupational therapy)
  • Dietary therapy (Modified Atkins diet / ketogenic diet)
  • Immunologic monitoring and management (screening for hypogammaglobulinemia; management of autoimmune cytopenias)

13. Prevention

Primary prevention (preventing occurrence) is not generally applicable for a de novo-dominant/X-linked congenital syndrome; however, secondary/tertiary prevention through surveillance and complication prevention is implied in management frameworks and supported by high rates of infection susceptibility and immune abnormalities. (margot2020immunopathologicalmanifestationsin pages 1-2, dugan2021kabukisyndrome pages 1-3)

14. Other Species / Natural Disease

No naturally occurring non-human Kabuki syndrome cases were retrieved in this run.

15. Model Organisms

15.1 Mouse models (key recent 2023–2024 studies)

  • KS1 hearing model: Kmt2d+/βGeo mice show progressive hearing impairment; ABR thresholds diverge after hearing onset and DPOAEs are diminished at multiple frequencies, consistent with outer hair cell dysfunction despite no gross cochlear malformations on micro-CT. (kalinousky2023kmt2ddeficiencycauses pages 7-9)
  • KS2 growth model: Kdm6a tm1d/+ mice exhibit postnatal growth deficiency with shortened long bones and growth-plate hypertrophic-zone reductions; Kdm6a−/− and Kmt2d−/− chondrocyte models show convergent transcriptomic changes. (gao2024growthdeficiencyin pages 3-6)
  • Cell-type chromatin comparisons: ATAC-seq across neurons vs B/T cells demonstrates cell-context-specific chromatin disruptions (neuronal CpG-island/aging enrichment), informing translational interpretation of blood-derived episignatures. (boukas2024neuronspecificchromatindisruption pages 1-2)

16. Recent Developments (2023–2024 highlights)

  1. Human epigenomic profiling in KS PBMCs links KMT2D haploinsufficiency to enhancer dysregulation at immune genes, with super-enhancer overlap (~31%) and metabolic gene upregulation. (Jung et al., 2023; https://doi.org/10.1101/2022.10.25.22280882). (jung2023characterizingthemolecular pages 1-5)
  2. T-cell intrinsic mechanism in KS: KMT2D control of leukocyte integrins and migration/egress programs, with corroborating human peripheral T-cell shifts (reduced naïve/RTE, expanded memory). (Potter et al., 2024; https://doi.org/10.3389/fimmu.2024.1341745). (potter2024kmt2dregulatesactivation pages 14-16, potter2024kmt2dregulatesactivation pages 1-2)
  3. Neuron-specific chromatin disruption suggests mechanistic differences across tissues and cautions in interpreting blood episignatures mechanistically for neurodevelopment. (Boukas et al., 2024; https://doi.org/10.1101/gr.278416.123). (boukas2024neuronspecificchromatindisruption pages 1-2)
  4. KS2 skeletal mechanism and convergence with KS1 via shared chondrocyte differentiation programs and growth-plate pathology. (Gao et al., 2024; https://doi.org/10.1371/journal.pgen.1011310). (gao2024growthdeficiencyin pages 3-6)
  5. Dietary intervention translational efforts: 2024 multi-omics report linking ribosomal protein dysregulation to KMT2D KS and describing Modified Atkins/ketogenic diet-associated molecular and cognitive changes (hypothesis-generating) plus an adult MAD clinical trial completed (NCT04722315). (tsang2024ketogenicdietmodifies pages 8-10, NCT04722315 chunk 1)

17. Notes on evidence gaps for this run

  • ICD-10/ICD-11, MeSH, Orphanet identifiers were not present in retrieved sources; they should be added from dedicated ontology resources (Orphanet/MeSH/ICD) in a follow-on extraction step.
  • Survival/life expectancy statistics were not captured in the extracted evidence; robust natural history cohorts would be needed.
  • Differential diagnosis lists and standardized care pathways were not extracted in this run.

References

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