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2
Definitions
10
Pathophys.
1
Histopath.
7
Phenotypes
2
Gaps
23
Pathograph
1
Genes
3
Medical Actions
2
Subtypes
1
Deep Research
📘

Definitions

2
Clinical framing of acute inorganic arsenic poisoning
A compatible acute exposure followed by gastrointestinal and cardiovascular toxicity, with exposure supported by appropriately interpreted arsenic measurements, defines the acute clinical syndrome.
CASE_DEFINITION Acute high-dose inorganic arsenic exposure
Show evidence (1 reference)
PMID:15533028 SUPPORT Human Clinical
"Classical symptoms of an acute arsenicum (As) poisoning such as gastrointestinal cramps, vomiting, diarrhea, ECG changes and disturbed liver function tests were observed."
This acute arsenic-trioxide case directly documents the characteristic gastrointestinal, cardiac, and hepatic presentation.
Clinical framing of chronic inorganic arsenicosis
Chronic arsenicosis is a clinical toxicity syndrome in a person with sustained inorganic arsenic exposure, commonly identified by characteristic melanosis and keratotic skin lesions rather than by exposure alone.
CASE_DEFINITION Chronic environmental inorganic arsenic exposure
Show evidence (1 reference)
PMID:11218669 SUPPORT Human Clinical
"Chronic arsenic toxicity (CAT) manifests predominantly as cutaneous lesions in the form of melanosis, keratosis and neoplastic changes."
The human biopsy series directly identifies melanosis and keratosis as predominant manifestations of chronic arsenic toxicity.

Subtypes

2
Acute inorganic arsenic poisoning
Toxicity following a large, usually ingested, dose of inorganic arsenic, with early gastrointestinal and hemodynamic manifestations and possible delayed neuropathy.
Show evidence (1 reference)
PMID:3578979 SUPPORT Human Clinical
"A 21-year-old man presented in shock after ingesting 2 g of arsenic trioxide."
Directly supports the acute high-dose ingestion subtype.
Chronic inorganic arsenicosis
Clinical toxicity arising during sustained inorganic arsenic exposure, particularly through drinking water, with characteristic cutaneous lesions.
Show evidence (1 reference)
PMID:21576319 SUPPORT Human Clinical
"Chronic arsenic exposure from drinking water was associated with increased incidence of skin lesions, even at low levels of arsenic exposure (<100 μg/L)."
The prospective cohort supports chronic drinking-water exposure as a cause of incident arsenical skin lesions.
?

Discussions and Knowledge Gaps

2
Which dithiol chelator, timing, and regimen improve patient-centered outcomes in severe acute inorganic arsenic poisoning beyond modern supportive care?
KNOWLEDGE GAP OPEN gap_arsenic_acute_chelation_outcomes
Attached to
treatments#Early dithiol chelation for severe acute poisoning
Acute treatment reports combine chelation with resuscitation, gastrointestinal decontamination, and other interventions, preventing a clean estimate of comparative efficacy. Chronic arsenicosis should not be used as a proxy indication: a randomized placebo-controlled DMSA trial did not show clinical, biochemical, or histopathologic benefit.
Show evidence (2 references)
PMID:9865236 SUPPORT Human Clinical
"Under the conditions of this study, 2,3-dimercaptosuccinic acid was not effective in producing any clinical or biochemical benefit or any histopathological improvement of skin lesions in patients with chronic arsenicosis."
The randomized trial directly establishes that chronic DMSA treatment did not provide the asserted benefits under the studied regimen.
PMID:15533028 PARTIAL Human Clinical
"The patient survived this massive dose of ingested inorganic As with only polyneuropathy one year later."
Survival after multiple simultaneous interventions motivates study but does not isolate chelator efficacy.
To what extent do PDH inhibition and ROS/RNS-mediated PARP-1 impairment drive the acute and chronic organ syndromes in exposed humans at clinically relevant internal doses?
HUMAN MODEL MISMATCH OPEN gap_arsenic_molecular_human_translation
The retained proximal mechanisms are supported by purified-enzyme, cell, and animal experiments, while the direct quantitative bridge to human organ injury remains unresolved. The graph therefore does not connect these molecular nodes directly to cancer, neuropathy, or cutaneous lesions.
Show evidence (2 references)
PMID:9806419 SUPPORT In Vitro
"Our results show that direct enzyme inhibition is not a common toxic effect of As and that only a few sensitive enzymes are responsible for arsenic-induced cellular toxicity."
Directly supports limiting the breadth of the enzyme-inhibition model.
PMID:23602911 SUPPORT In Vitro
"Most importantly, arsenite incubation with purified PARP-1 protein in vitro did not alter PARP-1 activity or DNA-binding ability, whereas hydrogen peroxide or NONOate retained PARP-1 inhibitory activity."
Supports an indirect ROS/RNS-mediated cellular mechanism and the need to test its relevance in human tissues.

Pathophysiology

10
Systemic inorganic arsenic exposure
Absorbed inorganic arsenic is the initiating toxic exposure. Dose, chemical species, route, and duration distinguish severe acute poisoning from chronic arsenicosis; exposure itself is not equivalent to clinical disease.
Show evidence (2 references)
PMID:3578979 SUPPORT Human Clinical
"Hemodynamic and laboratory data are presented illustrating the multisystem toxicities of inorganic arsenic."
Supports inorganic arsenic as the initiating exposure in acute multisystem toxicity.
PMID:21576319 SUPPORT Human Clinical
"The authors aimed to evaluate the association between arsenic exposure and skin lesion incidence among participants in the Health Effects of Arsenic Longitudinal Study (HEALS)."
Supports the initiating exposure in the chronic human cohort.
AS3MT-linked arsenic biotransformation
AS3MT-region variation changes the distribution of urinary MMA and DMA in exposed humans. This establishes a human exposure-response modifier but does not make AS3MT a monogenic cause of arsenicosis.
AS3MT hgnc:17452
detoxification of arsenic-containing substance GO:0071722 ⚠ ABNORMAL
Show evidence (1 reference)
PMID:22383894 SUPPORT Human Clinical
"In a follow-up analysis of 1,085 individuals with arsenic-induced premalignant skin lesions (the classical sign of arsenic toxicity) and 1,794 controls, we show that one of these five variants (rs9527) is also associated with skin lesion risk (P = 0.0005)."
Human association data connect the AS3MT-region signal to both metabolite proportions and arsenical skin-lesion susceptibility.
MMA(III)-sensitive pyruvate dehydrogenase inhibition
Pyruvate dehydrogenase is among the purified enzymes sensitive to micromolar arsenic, and MMA(III) inhibited PDH in animal tissue and purified-enzyme assays. These data do not justify a universal sulfhydryl-enzyme blockade model for human poisoning.
pyruvate metabolic process GO:0006090 ↓ DECREASED
Show evidence (2 references)
PMID:9806419 SUPPORT In Vitro
"Only pyruvate dehydrogenase, one of eight purified enzymes examined so far, is inhibited by micromolar arsenic."
Supports selective PDH sensitivity while constraining broader enzyme-inhibition claims.
PMID:11409934 SUPPORT In Vitro
"To inhibit activity of purified porcine heart PDH activity by 50%, the concentrations (mean +/- SE) of MMA(III) as methylarsine oxide and arsenite were 17.6 +/- 4.1 and 106.1 +/- 19.8 microM, respectively."
Quantifies greater PDH inhibition by MMA(III) than arsenite in the purified-enzyme assay.
Arsenite-generated oxidative and nitrosative stress
In cultured cells, arsenite-generated reactive oxygen and nitrogen species mediate downstream loss of PARP-1 zinc and activity.
response to oxidative stress GO:0006979 ↑ INCREASED
Show evidence (1 reference)
PMID:23602911 SUPPORT In Vitro
"Furthermore, the effects of arsenite on PARP-1 activity, DNA binding, and zinc content were partially reversed by the antioxidant ascorbic acid, catalase, and the NOS inhibitor, aminoguanidine."
Reversal by antioxidant and NOS-directed interventions supports a mediated role for ROS/RNS in this cellular model.
PARP-1 zinc loss and activity impairment
Arsenite-induced ROS/RNS reduce PARP-1 zinc content, DNA binding, and enzymatic activity in cells. This is a mechanistic observation relevant to DNA-repair impairment, not direct proof of a clinical cancer trajectory.
DNA repair GO:0006281 ↓ DECREASED
Show evidence (1 reference)
PMID:23602911 SUPPORT In Vitro
"These results strongly suggest that cellular generation of ROS/RNS plays an important role in arsenite inhibition of PARP-1 activity, leading to the loss of PARP-1 DNA-binding ability and enzymatic activity."
Supports the retained PARP-1 impairment mechanism and its model-system boundary.
Acute gastrointestinal and hemodynamic toxicity
Large acute inorganic arsenic ingestions can produce cramps, vomiting, diarrhea, oliguria, shock, and multisystem toxicity.
Show evidence (1 reference)
PMID:12793928 SUPPORT Human Clinical
"Initially, the patient developed signs of acute hemodynamic compromise with tachycardia, hypertension, gastrointestinal symptoms, and poor urine output."
Supports combined gastrointestinal and hemodynamic toxicity in acute poisoning.
Cardiac repolarization disturbance
Arsenic intoxication can markedly prolong the QT-U interval and cause torsade de pointes.
Show evidence (1 reference)
PMID:1689832 SUPPORT Human Clinical
"Reported in this study are two cases of arsenic poisoning causing torsade de pointes."
Supports clinically consequential repolarization toxicity.
Delayed peripheral axonopathy
A delayed axonal peripheral neuropathy can follow a single acute dose, with sensory nerve action-potential abnormalities and incomplete long-term recovery.
neuron CL:0000540
Show evidence (1 reference)
PMID:196051 SUPPORT Human Clinical
"Sural nerve biopsies from 2 patients showed axonal degeneration, which was at an early stage in some fibres, even 10 weeks after intoxication."
Human nerve biopsies establish axonal degeneration after acute intoxication.
Chronic cutaneous toxicity
Sustained inorganic arsenic exposure causes melanotic and keratotic skin lesions with epidermal proliferative changes.
keratinocyte CL:0000312 melanocyte CL:0000148
skin of body UBERON:0002097
Show evidence (1 reference)
PMID:21576319 SUPPORT Human Clinical
"Multivariate-adjusted hazard ratios for incident skin lesions comparing 10.1-50.0, 50.1-100.0, 100.1-200.0, and ≥200.1 μg/L with ≤10.0 μg/L of well water arsenic exposure were 1.17 (95% confidence interval (CI): 0.92, 1.49), 1.69 (95% CI: 1.33, 2.14), 1.97 (95% CI: 1.58, 2.46), and 2.98 (95% CI:..."
Prospective dose-response data support chronic arsenical cutaneous toxicity.
Hepatic biochemical injury
Higher chronic arsenic exposure is associated with increased ALP, AST, and ALT activities. These cross-sectional biochemical associations do not by themselves establish chronic liver failure or fibrosis.
Show evidence (1 reference)
PMID:21740555 SUPPORT Human Clinical
"Further, this study revealed a novel exposure- and dose- response relationship between arsenic exposure metrics and serum hepatic enzyme activity."
Supports a biochemical exposure-response relationship while preserving the study's cross-sectional boundary.

Histopathology

1
Hyperkeratotic and proliferative epidermal change
Biopsied arsenical skin lesions can show hyperkeratosis, parakeratosis, acanthosis, papillomatosis, rete-ridge elongation, increased basal pigmentation, and sometimes dysplasia; the pattern is supportive rather than independently pathognomonic.
Show evidence (1 reference)
PMID:11218669 SUPPORT Human Clinical
"Histological study of H/E stained sections showed--hyperkeratosis in 13, parakeratosis in 13, acanthosis in 12, papillomatosis in 24, elongation of reteridges in 21, increased basal pigmentation in 27 and dysplastic changes in 8 cases."
Directly supports the retained histopathologic features in 42 human biopsies.

Pathograph

Use the checkboxes to hide or show graph categories. Hover nodes for evidence and cross-linked metadata.
Pathograph: causal mechanism network for Arsenic Poisoning Interactive directed graph showing how pathophysiology mechanisms, phenotypes, genetic factors and variants, experimental models, environmental triggers, and treatments relate through causal and linked edges.

Phenotypes

7
Cardiovascular 2
Shock Shock HP:0031273
Temporal: ACUTE
Show evidence (1 reference)
PMID:3578979 SUPPORT Human Clinical
"A 21-year-old man presented in shock after ingesting 2 g of arsenic trioxide."
Directly supports shock after severe acute ingestion.
Prolonged QTc interval Prolonged QTc interval HP:0005184
Temporal: ACUTE
Show evidence (1 reference)
PMID:1689832 SUPPORT Human Clinical
"Furthermore, marked prolongation of the QT-U interval and the rarely observed phenomenon of T-U wave alternans are demonstrated."
Directly supports marked repolarization-interval prolongation.
Digestive 2
Vomiting Vomiting HP:0002013
Temporal: ACUTE
Show evidence (1 reference)
PMID:15533028 SUPPORT Human Clinical
"Classical symptoms of an acute arsenicum (As) poisoning such as gastrointestinal cramps, vomiting, diarrhea, ECG changes and disturbed liver function tests were observed."
Directly supports vomiting in acute poisoning.
Diarrhea Diarrhea HP:0002014
Temporal: ACUTE
Show evidence (1 reference)
PMID:15533028 SUPPORT Human Clinical
"Classical symptoms of an acute arsenicum (As) poisoning such as gastrointestinal cramps, vomiting, diarrhea, ECG changes and disturbed liver function tests were observed."
Directly supports diarrhea in acute poisoning.
Integument 2
Chronic hyperpigmentation Hyperpigmentation of the skin HP:0000953
Temporal: CHRONIC
Show evidence (1 reference)
PMID:11218669 SUPPORT Human Clinical
"Chronic arsenic toxicity (CAT) manifests predominantly as cutaneous lesions in the form of melanosis, keratosis and neoplastic changes."
Directly supports melanosis in chronic arsenic toxicity.
Arsenical palmar keratosis Palmoplantar keratoderma HP:0000982
Temporal: CHRONIC
Show evidence (1 reference)
PMID:40583662 SUPPORT Human Clinical
"Thirty patients of moderate palmar arsenical keratosis from an arsenic-affected area at Cumilla in Bangladesh were recruited randomly based on inclusion and exclusion criteria."
Directly documents palmar arsenical keratosis in a primary human treatment cohort.
Nervous System 1
Delayed peripheral neuropathy Peripheral neuropathy HP:0009830
Temporal: SUBACUTE
Show evidence (1 reference)
PMID:196051 SUPPORT Human Clinical
"Four patients are described who developed a peripheral neuropathy 10 days to 3 weeks after ingestion of a single dose of arsenic."
Directly supports the delayed peripheral neuropathy phenotype.
🧬

Genetic Associations

1
AS3MT (Exposure-response modifier of arsenic metabolism and arsenical skin-lesion risk)
Gene: AS3MT hgnc:17452 relationship_type: SUSCEPTIBILITY
Show evidence (1 reference)
PMID:22383894 SUPPORT Human Clinical
"In a follow-up analysis of 1,085 individuals with arsenic-induced premalignant skin lesions (the classical sign of arsenic toxicity) and 1,794 controls, we show that one of these five variants (rs9527) is also associated with skin lesion risk (P = 0.0005)."
Directly supports an AS3MT-region susceptibility association under arsenic exposure.
💊

Medical Actions

3
Exposure cessation and arsenic-safe water
Category: Therapeutic Action: chemical exposure avoidance Ontology label: Lifestyle Therapy NCIT:C15900
Remove the exposure source. For chronic drinking-water arsenicosis, provide reliably arsenic-safe water and prevent renewed exposure. Skin lesions can improve over years, but established severe disease may persist.
Mechanism Target:
INHIBITS Systemic inorganic arsenic exposure — Preventing further ingestion reduces ongoing systemic arsenic exposure.
Show evidence (1 reference)
PMID:25161968 SUPPORT Human Clinical
"All the participants who had arsenic contaminated drinking water source in their houses were supplied with arsenic removal filters for getting arsenic-free water during the follow-up period."
Directly documents exposure mitigation in the human follow-up study.
Show evidence (1 reference)
PMID:25161968 SUPPORT Human Clinical
"In participants belonging to Cohort-I, the skin score was found to improve significantly at the end of each year, and it was found to be reduced significantly from 2.17 ± 1.09 to 1.23 ± 1.17; P < 0.001 at the end of 3 year's intervention study indicating beneficial effect of safe water on skin lesions."
Supports clinical improvement after provision of arsenic-safe water.
Early supportive resuscitation and intensive monitoring
Category: Therapeutic Action: supportive care Ontology label: Supportive Care NCIT:C15747
Acute severe poisoning requires immediate restoration and maintenance of cardiac output and renal perfusion, with continuous cardiovascular, electrolyte, urine-output, and acid-base monitoring.
Mechanism Target:
RESTORES Acute gastrointestinal and hemodynamic toxicity — Resuscitation restores organ perfusion during early hemodynamic compromise.
Show evidence (1 reference)
PMID:12793928 SUPPORT Human Clinical
"Recovery from arsenic poisoning was attributable to the restoration and maintenance of adequate cardiac output and renal perfusion in early shock, which allowed depot intramuscular British anti-Lewisite to circulate and eliminate the poison."
Directly supports early restoration of perfusion in this acute survivor.
Show evidence (1 reference)
PMID:12793928 SUPPORT Human Clinical
"Continuous monitoring in the pediatric intensive care unit included continuous electrocardiogram, arterial blood pressure, urine output, vital signs, arterial blood gases, serum and urine arsenic concentrations, electrolytes, electromyography, and determination of arsenic toxicokinetics."
Directly supports the intensive monitoring components retained here.
Early dithiol chelation for severe acute poisoning
Category: Therapeutic Action: chelator agent therapy Ontology label: Pharmacotherapy NCIT:C15986
Agent: dimercaprol CHEBI:64198 succimer CHEBI:63623 2,3-disulfanylpropane-1-sulfonic acid CHEBI:888
Dimercaprol, succimer, or DMPS may be considered early for severe acute inorganic arsenic poisoning with toxicology consultation. Human evidence is largely multi-intervention case experience, so this entry does not rank the agents or assert controlled outcome benefit.
Mechanism Target:
INHIBITS Systemic inorganic arsenic exposure — Dithiol ligands can bind arsenic species and promote urinary excretion.
Show evidence (1 reference)
PMID:12387631 SUPPORT Human Clinical
"The finding of the DMPS-MMA(III) complex in human urine after DMPS treatment provides an explanation for the inhibition of arsenic methylation by DMPS."
Demonstrates a human urinary DMPS-arsenic complex supporting the chelation mechanism.
Show evidence (2 references)
PMID:12793928 PARTIAL Human Clinical
"He was stabilized with fluid resuscitation, placed on a sodium bicarbonate intravenous drip, and treated with intramuscular dimercaprol (British anti-Lewisite), 5 mg/kg every 6 hrs for 3 days."
Documents early dimercaprol use in a survivor but cannot isolate chelation efficacy from simultaneous resuscitation and other treatment.
PMID:15533028 PARTIAL Human Clinical
"Forced diuresis, BAL (2,3-dimercaptopropanol) and DMSA (meso-2,3-dimercaptosuccinic acid) were started and therapy to enhance the formation of methylated As derivatives, which are potentially less toxic and which can be excreted more easily, was then administered."
Documents BAL and DMSA within a successful multi-intervention case, not a controlled estimate of benefit.
🌍

Environmental Factors

2
Chronic ingestion of arsenic-contaminated drinking water
exposure to arsenic in water via ingestion ECTO:0080000
Sustained ingestion of inorganic arsenic in groundwater is the central exposure context for chronic arsenicosis.
Show evidence (1 reference)
PMID:21576319 SUPPORT Human Clinical
"Chronic arsenic exposure from drinking water was associated with increased incidence of skin lesions, even at low levels of arsenic exposure (<100 μg/L)."
Prospective human data support contaminated drinking water as a chronic causal exposure.
Acute high-dose inorganic arsenic ingestion
exposure to arsenic ECTO:9000032
Accidental or intentional ingestion of a concentrated inorganic arsenic compound can produce life-threatening acute poisoning.
Show evidence (2 references)
PMID:3578979 SUPPORT Human Clinical
"A 21-year-old man presented in shock after ingesting 2 g of arsenic trioxide."
Directly supports concentrated inorganic arsenic ingestion as an acute exposure.
PMID:12793928 SUPPORT Human Clinical
"A 22-month-old boy ingested approximately twice the estimated lethal dose of arsenic trioxide (As(2)O(3)) ant bait."
Directly supports accidental pediatric ingestion of an arsenical insecticide.
🔬

Biochemical Markers

2
Total and speciated urinary arsenic (INCREASED)
Context: Total urinary arsenic reflects recent exposure but can rise markedly after seafood because of arsenobetaine. Speciation of inorganic arsenic, MMA, DMA, and arsenobetaine is necessary when the total is elevated; no universal diagnostic threshold is asserted here.
Pathograph Readouts
Readout Of Systemic inorganic arsenic exposure Threshold Dependent Diagnostic
The inorganic and methylated species support recent inorganic exposure, whereas arsenobetaine-dominant elevation can reflect seafood.
Show evidence (1 reference)
PMID:18430299 SUPPORT Human Clinical
"Two case studies underline the need for As speciation, especially when total urinary arsenic concentrations are elevated."
Directly supports species-level interpretation of an elevated total.
Show evidence (1 reference)
PMID:18430299 SUPPORT Human Clinical
"A maximum enhancement of total As from 1 up to 2,200 microg/L (2,000 microg/L for As-B) was observed after a normal fish meal."
Demonstrates the magnitude of seafood-related confounding of total urinary arsenic.
Serum ALP, AST, and ALT activities (INCREASED)
Context: Increased activities correlate with higher chronic exposure in the cited cross-sectional cohort; they are nonspecific injury markers, not a diagnosis of arsenicosis or liver failure.
Pathograph Readouts
Correlates With Hepatic biochemical injury Positive Diagnostic
Higher hepatic enzyme activities track a biochemical liver-injury association in exposed populations.
Show evidence (1 reference)
PMID:21740555 SUPPORT Human Clinical
"the respective activities of ALP, AST and ALT were found to be significantly increased in the high-exposure groups compared to the lowest-exposure groups before and after adjustments were made for different covariates."
Directly supports the exposure-stratified biochemical association.
Show evidence (1 reference)
PMID:21740555 SUPPORT Human Clinical
"Elevated serum hepatic enzyme activities in the higher exposure gradients provided new insights into arsenic-induced liver toxicity that might be helpful for the early prognosis of arsenic-induced liver diseases."
Supports increased hepatic enzyme activity as an exposure-associated biochemical finding.
{ }

Source YAML

click to show
name: Arsenic Poisoning
creation_date: '2026-02-11T21:06:43Z'
category: Environmental
categories:
- Toxic Exposure Disorder
- Heavy Metal Poisoning
- Environmental Health Disorder
synonyms:
- arsenicosis
- arsenic intoxication
- inorganic arsenic poisoning
description: >-
  Arsenic poisoning is a toxic disorder caused by clinically significant
  exposure to inorganic arsenic. Acute high-dose ingestion can produce severe
  gastrointestinal illness, hemodynamic compromise, cardiac repolarization
  abnormalities, multisystem toxicity, and death; peripheral neuropathy can
  appear after a delay. Chronic exposure, most often through contaminated
  drinking water, is classically associated with hyperpigmentation and
  palmoplantar keratosis. Total urinary arsenic can be misleading after seafood
  consumption because arsenobetaine can raise the total, so arsenic speciation
  is central to exposure assessment. Long-latency
  arsenic-related malignancies are modeled separately in Arsenic Related
  Cancers; this entry does not infer cancer from short-term poisoning findings.
definitions:
- name: Clinical framing of acute inorganic arsenic poisoning
  definition_type: CASE_DEFINITION
  description: >-
    A compatible acute exposure followed by gastrointestinal and cardiovascular
    toxicity, with exposure supported by appropriately interpreted arsenic
    measurements, defines the acute clinical syndrome.
  scope: Acute high-dose inorganic arsenic exposure
  evidence:
  - reference: PMID:15533028
    reference_title: Survival after a lethal dose of arsenic trioxide.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Classical symptoms of an acute arsenicum (As) poisoning such as gastrointestinal cramps, vomiting, diarrhea, ECG changes and disturbed liver function tests were observed.
    explanation: >-
      This acute arsenic-trioxide case directly documents the characteristic
      gastrointestinal, cardiac, and hepatic presentation.
- name: Clinical framing of chronic inorganic arsenicosis
  definition_type: CASE_DEFINITION
  description: >-
    Chronic arsenicosis is a clinical toxicity syndrome in a person with
    sustained inorganic arsenic exposure, commonly identified by characteristic
    melanosis and keratotic skin lesions rather than by exposure alone.
  scope: Chronic environmental inorganic arsenic exposure
  evidence:
  - reference: PMID:11218669
    reference_title: Histopathology of skin lesions in chronic arsenic toxicity--grading of changes and study of proliferative markers.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Chronic arsenic toxicity (CAT) manifests predominantly as cutaneous lesions in the form of melanosis, keratosis and neoplastic changes.
    explanation: >-
      The human biopsy series directly identifies melanosis and keratosis as
      predominant manifestations of chronic arsenic toxicity.
parents:
- heavy metal poisoning
has_subtypes:
- name: Acute
  display_name: Acute inorganic arsenic poisoning
  description: >-
    Toxicity following a large, usually ingested, dose of inorganic arsenic,
    with early gastrointestinal and hemodynamic manifestations and possible
    delayed neuropathy.
  evidence:
  - reference: PMID:3578979
    reference_title: Acute arsenic ingestion.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      A 21-year-old man presented in shock after ingesting 2 g of arsenic trioxide.
    explanation: Directly supports the acute high-dose ingestion subtype.
- name: Chronic
  display_name: Chronic inorganic arsenicosis
  description: >-
    Clinical toxicity arising during sustained inorganic arsenic exposure,
    particularly through drinking water, with characteristic cutaneous lesions.
  evidence:
  - reference: PMID:21576319
    reference_title: A prospective study of arsenic exposure from drinking water and incidence of skin lesions in Bangladesh.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Chronic arsenic exposure from drinking water was associated with increased incidence of skin lesions, even at low levels of arsenic exposure (<100 μg/L).
    explanation: >-
      The prospective cohort supports chronic drinking-water exposure as a cause
      of incident arsenical skin lesions.
progression:
- phase: Early acute gastrointestinal and hemodynamic toxicity
  subtype: Acute
  notes: >-
    Gastrointestinal symptoms and hemodynamic compromise can occur early after a
    large ingestion and require immediate stabilization and monitoring.
  evidence:
  - reference: PMID:12793928
    reference_title: 'Treatment and toxicokinetics of acute pediatric arsenic ingestion: danger of arsenic insecticides in children.'
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Initially, the patient developed signs of acute hemodynamic compromise with tachycardia, hypertension, gastrointestinal symptoms, and poor urine output.
    explanation: The case directly establishes the early acute phase.
- phase: Delayed peripheral neuropathy after a single acute exposure
  subtype: Acute
  duration: Neurologic abnormalities can persist for years
  notes: >-
    Peripheral neuropathy can begin 10 days to 3 weeks after ingestion and may
    improve only slowly and incompletely.
  evidence:
  - reference: PMID:196051
    reference_title: Peripheral neuropathy following a single exposure to arsenic. Clincal course in four patients with electrophysiological and histological studies.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Four patients are described who developed a peripheral neuropathy 10 days to 3 weeks after ingestion of a single dose of arsenic. All improved slowly, but after 6 to 8 years 3 of them still had abnormal neurological symptoms and signs.
    explanation: >-
      The longitudinal case series directly supplies the onset window and
      persistent course of delayed neuropathy.
- phase: Chronic cutaneous disease during sustained exposure and after mitigation
  subtype: Chronic
  duration: Years
  notes: >-
    Skin lesions arise during sustained drinking-water exposure. Providing
    arsenic-safe water can improve lesion scores over several years, although
    severe lesions and systemic disease may persist.
  evidence:
  - reference: PMID:25161968
    reference_title: 'Effect of Safe Water on Arsenicosis: A Follow-up Study.'
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      In participants belonging to Cohort-I, the skin score was found to improve significantly at the end of each year, and it was found to be reduced significantly from 2.17 ± 1.09 to 1.23 ± 1.17; P < 0.001 at the end of 3 year's intervention study indicating beneficial effect of safe water on skin lesions.
    explanation: >-
      The three-year intervention follow-up supports improvement after exposure
      mitigation without implying that all established disease reverses.
clinical_burden:
  burden_level: HIGH
  rationale: >-
    Severe acute poisoning can be fatal despite intensive care, and delayed
    neurologic or chronic cutaneous injury can remain clinically important for
    years.
  evidence:
  - reference: PMID:3578979
    reference_title: Acute arsenic ingestion.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      He died within 37 hours despite intensive treatment that included intramuscular dimercaprol and hemodialysis.
    explanation: Documents the mortality risk of severe acute ingestion.
  - reference: PMID:196051
    reference_title: Peripheral neuropathy following a single exposure to arsenic. Clincal course in four patients with electrophysiological and histological studies.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      All improved slowly, but after 6 to 8 years 3 of them still had abnormal neurological symptoms and signs.
    explanation: Documents long-term neurologic burden among survivors.
pathophysiology:
- name: Systemic inorganic arsenic exposure
  biological_scale: ORGANISM
  mechanism_confidence: ESTABLISHED
  description: >-
    Absorbed inorganic arsenic is the initiating toxic exposure. Dose, chemical
    species, route, and duration distinguish severe acute poisoning from chronic
    arsenicosis; exposure itself is not equivalent to clinical disease.
  chemical_entities:
  - preferred_term: arsenic atom
    term:
      id: CHEBI:27563
      label: arsenic atom
  triggers:
  - preferred_term: exposure to arsenic
    term:
      id: ECTO:9000032
      label: exposure to arsenic
  downstream:
  - target: AS3MT-linked arsenic biotransformation
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    intermediate_mechanisms:
    - cellular uptake and enzymatic methylation of inorganic arsenic
    description: >-
      In exposed humans, variation near AS3MT strongly influences the urinary
      distribution of methylated arsenic species.
    evidence:
    - reference: PMID:22383894
      reference_title: Genome-wide association study identifies chromosome 10q24.32 variants associated with arsenic metabolism and toxicity phenotypes in Bangladesh.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Using data on urinary arsenic metabolite concentrations and approximately 300,000 genome-wide single nucleotide polymorphisms (SNPs) for 1,313 arsenic-exposed Bangladeshi individuals, we identified genome-wide significant association signals (P<5×10(-8)) for percentages of both monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) near the AS3MT gene (arsenite methyltransferase; 10q24.32), with five genetic variants showing independent associations.
      explanation: >-
        The human GWAS links AS3MT-region variation to arsenic metabolite
        proportions under exposure.
  - target: Arsenite-generated oxidative and nitrosative stress
    causal_link_type: DIRECT
    description: Cellular arsenite exposure generates reactive oxygen and nitrogen species.
    evidence:
    - reference: PMID:23602911
      reference_title: Arsenite-induced ROS/RNS generation causes zinc loss and inhibits the activity of poly(ADP-ribose) polymerase-1.
      supports: SUPPORT
      evidence_source: IN_VITRO
      snippet: >-
        We report herein that arsenite-generated ROS/RNS inhibits PARP-1 activity in cells.
      explanation: Directly supports arsenite-triggered cellular ROS/RNS generation.
  - target: Acute gastrointestinal and hemodynamic toxicity
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: >-
      Large acute ingestions produce gastrointestinal illness and cardiovascular
      compromise through multiple incompletely resolved intermediates.
    evidence:
    - reference: PMID:15533028
      reference_title: Survival after a lethal dose of arsenic trioxide.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Classical symptoms of an acute arsenicum (As) poisoning such as gastrointestinal cramps, vomiting, diarrhea, ECG changes and disturbed liver function tests were observed.
      explanation: >-
        The acute ingestion case directly links arsenic trioxide to the retained
        gastrointestinal syndrome.
    - reference: PMID:3578979
      reference_title: Acute arsenic ingestion.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        A 21-year-old man presented in shock after ingesting 2 g of arsenic trioxide.
      explanation: Directly links acute arsenic-trioxide ingestion to shock.
  - target: Cardiac repolarization disturbance
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: Arsenic intoxication can markedly prolong repolarization and trigger torsade de pointes.
    evidence:
    - reference: PMID:1689832
      reference_title: Torsade de pointes and T-U wave alternans associated with arsenic poisoning.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Reported in this study are two cases of arsenic poisoning causing torsade de pointes. Furthermore, marked prolongation of the QT-U interval and the rarely observed phenomenon of T-U wave alternans are demonstrated.
      explanation: The two cases directly support arsenic-associated repolarization toxicity.
  - target: Delayed peripheral axonopathy
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: Peripheral nerve injury may appear after a symptom-free interval following acute ingestion.
    evidence:
    - reference: PMID:196051
      reference_title: Peripheral neuropathy following a single exposure to arsenic. Clincal course in four patients with electrophysiological and histological studies.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Sural nerve biopsies from 2 patients showed axonal degeneration, which was at an early stage in some fibres, even 10 weeks after intoxication.
      explanation: >-
        Human biopsy evidence directly links the post-ingestion neuropathy to
        peripheral axonal degeneration.
  - target: Chronic cutaneous toxicity
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: Sustained drinking-water arsenic exposure increases incident arsenical skin lesions.
    evidence:
    - reference: PMID:21576319
      reference_title: A prospective study of arsenic exposure from drinking water and incidence of skin lesions in Bangladesh.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Chronic arsenic exposure from drinking water was associated with increased incidence of skin lesions, even at low levels of arsenic exposure (<100 μg/L).
      explanation: The prospective cohort directly supports the exposure-to-skin-lesion edge.
  - target: Hepatic biochemical injury
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: Higher chronic exposure is associated with increased serum hepatic enzyme activities.
    evidence:
    - reference: PMID:21740555
      reference_title: 'Dose-response relationship between arsenic exposure and the serum enzymes for liver function tests in the individuals exposed to arsenic: a cross sectional study in Bangladesh.'
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        the respective activities of ALP, AST and ALT were found to be significantly increased in the high-exposure groups compared to the lowest-exposure groups before and after adjustments were made for different covariates.
      explanation: >-
        The exposure-stratified human study directly supports a dose-related
        biochemical liver-injury association.
  evidence:
  - reference: PMID:3578979
    reference_title: Acute arsenic ingestion.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Hemodynamic and laboratory data are presented illustrating the multisystem toxicities of inorganic arsenic.
    explanation: Supports inorganic arsenic as the initiating exposure in acute multisystem toxicity.
  - reference: PMID:21576319
    reference_title: A prospective study of arsenic exposure from drinking water and incidence of skin lesions in Bangladesh.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The authors aimed to evaluate the association between arsenic exposure and skin lesion incidence among participants in the Health Effects of Arsenic Longitudinal Study (HEALS).
    explanation: Supports the initiating exposure in the chronic human cohort.
- name: AS3MT-linked arsenic biotransformation
  biological_scale: MOLECULAR
  mechanism_confidence: PROVISIONAL
  description: >-
    AS3MT-region variation changes the distribution of urinary MMA and DMA in
    exposed humans. This establishes a human exposure-response modifier but does
    not make AS3MT a monogenic cause of arsenicosis.
  gene:
    preferred_term: AS3MT
    term:
      id: hgnc:17452
      label: AS3MT
  biological_processes:
  - preferred_term: detoxification of arsenic-containing substance
    modifier: ABNORMAL
    term:
      id: GO:0071722
      label: detoxification of arsenic-containing substance
  downstream:
  - target: MMA(III)-sensitive pyruvate dehydrogenase inhibition
    causal_link_type: INDIRECT_KNOWN_INTERMEDIATES
    intermediate_mechanisms:
    - production of trivalent monomethylated arsenic species
    description: >-
      Methylation can generate MMA(III), which inhibited pyruvate dehydrogenase
      more potently than arsenite in the studied systems.
    evidence:
    - reference: PMID:11409934
      reference_title: 'Monomethylarsonous acid (MMA(III)) and arsenite: LD(50) in hamsters and in vitro inhibition of pyruvate dehydrogenase.'
      supports: SUPPORT
      evidence_source: MODEL_ORGANISM
      snippet: >-
        These data demonstrate that MMA(III) is more toxic than inorganic arsenite, both in vivo and in vitro, and call into question the hypothesis that methylation of inorganic arsenic is a detoxication process.
      explanation: >-
        The comparative animal and enzyme data support toxicity of the
        methylated trivalent intermediate rather than uniform detoxification.
  evidence:
  - reference: PMID:22383894
    reference_title: Genome-wide association study identifies chromosome 10q24.32 variants associated with arsenic metabolism and toxicity phenotypes in Bangladesh.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      In a follow-up analysis of 1,085 individuals with arsenic-induced premalignant skin lesions (the classical sign of arsenic toxicity) and 1,794 controls, we show that one of these five variants (rs9527) is also associated with skin lesion risk (P = 0.0005).
    explanation: >-
      Human association data connect the AS3MT-region signal to both metabolite
      proportions and arsenical skin-lesion susceptibility.
- name: MMA(III)-sensitive pyruvate dehydrogenase inhibition
  biological_scale: MOLECULAR
  mechanism_confidence: PROVISIONAL
  description: >-
    Pyruvate dehydrogenase is among the purified enzymes sensitive to micromolar
    arsenic, and MMA(III) inhibited PDH in animal tissue and purified-enzyme
    assays. These data do not justify a universal sulfhydryl-enzyme blockade
    model for human poisoning.
  biological_processes:
  - preferred_term: pyruvate metabolic process
    modifier: DECREASED
    term:
      id: GO:0006090
      label: pyruvate metabolic process
  evidence:
  - reference: PMID:9806419
    reference_title: Arsenic toxicity is enzyme specific and its affects on ligation are not caused by the direct inhibition of DNA repair enzymes.
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      Only pyruvate dehydrogenase, one of eight purified enzymes examined so far, is inhibited by micromolar arsenic.
    explanation: Supports selective PDH sensitivity while constraining broader enzyme-inhibition claims.
  - reference: PMID:11409934
    reference_title: 'Monomethylarsonous acid (MMA(III)) and arsenite: LD(50) in hamsters and in vitro inhibition of pyruvate dehydrogenase.'
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      To inhibit activity of purified porcine heart PDH activity by 50%, the concentrations (mean +/- SE) of MMA(III) as methylarsine oxide and arsenite were 17.6 +/- 4.1 and 106.1 +/- 19.8 microM, respectively.
    explanation: Quantifies greater PDH inhibition by MMA(III) than arsenite in the purified-enzyme assay.
- name: Arsenite-generated oxidative and nitrosative stress
  biological_scale: CELLULAR
  mechanism_confidence: PROVISIONAL
  description: >-
    In cultured cells, arsenite-generated reactive oxygen and nitrogen species
    mediate downstream loss of PARP-1 zinc and activity.
  biological_processes:
  - preferred_term: response to oxidative stress
    modifier: INCREASED
    term:
      id: GO:0006979
      label: response to oxidative stress
  downstream:
  - target: PARP-1 zinc loss and activity impairment
    causal_link_type: DIRECT
    description: Arsenite-generated ROS/RNS decrease PARP-1 zinc content, DNA binding, and enzymatic activity.
    evidence:
    - reference: PMID:23602911
      reference_title: Arsenite-induced ROS/RNS generation causes zinc loss and inhibits the activity of poly(ADP-ribose) polymerase-1.
      supports: SUPPORT
      evidence_source: IN_VITRO
      snippet: >-
        Cellular exposure to arsenite, as well as hydrogen peroxide and NONOate (nitric oxide donor), decreased PARP-1 zinc content, enzymatic activity, and PARP-1 DNA binding.
      explanation: Directly supports the ROS/RNS-to-PARP-1 impairment edge in cells.
  evidence:
  - reference: PMID:23602911
    reference_title: Arsenite-induced ROS/RNS generation causes zinc loss and inhibits the activity of poly(ADP-ribose) polymerase-1.
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      Furthermore, the effects of arsenite on PARP-1 activity, DNA binding, and zinc content were partially reversed by the antioxidant ascorbic acid, catalase, and the NOS inhibitor, aminoguanidine.
    explanation: >-
      Reversal by antioxidant and NOS-directed interventions supports a mediated
      role for ROS/RNS in this cellular model.
- name: PARP-1 zinc loss and activity impairment
  biological_scale: MOLECULAR
  mechanism_confidence: PROVISIONAL
  description: >-
    Arsenite-induced ROS/RNS reduce PARP-1 zinc content, DNA binding, and
    enzymatic activity in cells. This is a mechanistic observation relevant to
    DNA-repair impairment, not direct proof of a clinical cancer trajectory.
  biological_processes:
  - preferred_term: DNA repair
    modifier: DECREASED
    term:
      id: GO:0006281
      label: DNA repair
  evidence:
  - reference: PMID:23602911
    reference_title: Arsenite-induced ROS/RNS generation causes zinc loss and inhibits the activity of poly(ADP-ribose) polymerase-1.
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      These results strongly suggest that cellular generation of ROS/RNS plays an important role in arsenite inhibition of PARP-1 activity, leading to the loss of PARP-1 DNA-binding ability and enzymatic activity.
    explanation: Supports the retained PARP-1 impairment mechanism and its model-system boundary.
- name: Acute gastrointestinal and hemodynamic toxicity
  biological_scale: ORGANISM
  mechanism_confidence: ESTABLISHED
  subtypes:
  - Acute
  description: >-
    Large acute inorganic arsenic ingestions can produce cramps, vomiting,
    diarrhea, oliguria, shock, and multisystem toxicity.
  downstream:
  - target: Vomiting
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: Acute inorganic arsenic ingestion produces vomiting.
    evidence:
    - reference: PMID:15533028
      reference_title: Survival after a lethal dose of arsenic trioxide.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Classical symptoms of an acute arsenicum (As) poisoning such as gastrointestinal cramps, vomiting, diarrhea, ECG changes and disturbed liver function tests were observed.
      explanation: Directly supports vomiting after acute arsenic-trioxide ingestion.
  - target: Diarrhea
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: Acute inorganic arsenic ingestion produces diarrhea.
    evidence:
    - reference: PMID:15533028
      reference_title: Survival after a lethal dose of arsenic trioxide.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Classical symptoms of an acute arsenicum (As) poisoning such as gastrointestinal cramps, vomiting, diarrhea, ECG changes and disturbed liver function tests were observed.
      explanation: Directly supports diarrhea after acute arsenic-trioxide ingestion.
  - target: Shock
    causal_link_type: INDIRECT_UNKNOWN_INTERMEDIATES
    description: Severe acute arsenic ingestion can cause shock.
    evidence:
    - reference: PMID:3578979
      reference_title: Acute arsenic ingestion.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        A 21-year-old man presented in shock after ingesting 2 g of arsenic trioxide.
      explanation: Directly supports shock after severe acute ingestion.
  evidence:
  - reference: PMID:12793928
    reference_title: 'Treatment and toxicokinetics of acute pediatric arsenic ingestion: danger of arsenic insecticides in children.'
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Initially, the patient developed signs of acute hemodynamic compromise with tachycardia, hypertension, gastrointestinal symptoms, and poor urine output.
    explanation: Supports combined gastrointestinal and hemodynamic toxicity in acute poisoning.
- name: Cardiac repolarization disturbance
  biological_scale: ORGANISM
  mechanism_confidence: ESTABLISHED
  subtypes:
  - Acute
  description: >-
    Arsenic intoxication can markedly prolong the QT-U interval and cause torsade
    de pointes.
  downstream:
  - target: Prolonged QTc interval
    causal_link_type: DIRECT
    description: Arsenic-associated repolarization disturbance manifests as marked QT-U prolongation.
    evidence:
    - reference: PMID:1689832
      reference_title: Torsade de pointes and T-U wave alternans associated with arsenic poisoning.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Thus, arsenic intoxication may be complicated by prolongation of the QT-U interval and torsade de pointes.
      explanation: Directly supports the electrocardiographic phenotype.
  evidence:
  - reference: PMID:1689832
    reference_title: Torsade de pointes and T-U wave alternans associated with arsenic poisoning.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Reported in this study are two cases of arsenic poisoning causing torsade de pointes.
    explanation: Supports clinically consequential repolarization toxicity.
- name: Delayed peripheral axonopathy
  biological_scale: TISSUE
  mechanism_confidence: ESTABLISHED
  subtypes:
  - Acute
  description: >-
    A delayed axonal peripheral neuropathy can follow a single acute dose, with
    sensory nerve action-potential abnormalities and incomplete long-term
    recovery.
  cell_types:
  - preferred_term: neuron
    term:
      id: CL:0000540
      label: neuron
  locations:
  - preferred_term: nerve
    term:
      id: UBERON:0001021
      label: nerve
  downstream:
  - target: Delayed peripheral neuropathy
    causal_link_type: DIRECT
    description: Peripheral axonal degeneration manifests as delayed neuropathy.
    evidence:
    - reference: PMID:196051
      reference_title: Peripheral neuropathy following a single exposure to arsenic. Clincal course in four patients with electrophysiological and histological studies.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Electrophysiological studies showed reduction of motor conduction velocity and marked abnormalities of sensory nerve action potentials.
      explanation: Electrophysiology directly supports the clinical neuropathy phenotype.
  evidence:
  - reference: PMID:196051
    reference_title: Peripheral neuropathy following a single exposure to arsenic. Clincal course in four patients with electrophysiological and histological studies.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Sural nerve biopsies from 2 patients showed axonal degeneration, which was at an early stage in some fibres, even 10 weeks after intoxication.
    explanation: Human nerve biopsies establish axonal degeneration after acute intoxication.
- name: Chronic cutaneous toxicity
  biological_scale: TISSUE
  mechanism_confidence: ESTABLISHED
  subtypes:
  - Chronic
  description: >-
    Sustained inorganic arsenic exposure causes melanotic and keratotic skin
    lesions with epidermal proliferative changes.
  cell_types:
  - preferred_term: keratinocyte
    term:
      id: CL:0000312
      label: keratinocyte
  - preferred_term: melanocyte
    term:
      id: CL:0000148
      label: melanocyte
  locations:
  - preferred_term: skin of body
    term:
      id: UBERON:0002097
      label: skin of body
  downstream:
  - target: Chronic hyperpigmentation
    causal_link_type: DIRECT
    description: Chronic arsenical cutaneous toxicity manifests as melanosis and increased basal pigmentation.
    evidence:
    - reference: PMID:11218669
      reference_title: Histopathology of skin lesions in chronic arsenic toxicity--grading of changes and study of proliferative markers.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Histological study of H/E stained sections showed--hyperkeratosis in 13, parakeratosis in 13, acanthosis in 12, papillomatosis in 24, elongation of reteridges in 21, increased basal pigmentation in 27 and dysplastic changes in 8 cases.
      explanation: Directly supports increased basal pigmentation in chronic arsenical skin lesions.
  - target: Arsenical palmar keratosis
    causal_link_type: DIRECT
    description: Chronic arsenical cutaneous toxicity can manifest as keratotic lesions on the palms.
    evidence:
    - reference: PMID:40583662
      reference_title: Comparison between Combination of Neem, Propylene Glycol and Salicylic Acid with Salicylic Acid Alone Topically in Arsenical Palmar Keratosis.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Thirty patients of moderate palmar arsenical keratosis from an arsenic-affected area at Cumilla in Bangladesh were recruited randomly based on inclusion and exclusion criteria.
      explanation: Directly documents palmar arsenical keratosis in a primary human treatment cohort.
  evidence:
  - reference: PMID:21576319
    reference_title: A prospective study of arsenic exposure from drinking water and incidence of skin lesions in Bangladesh.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Multivariate-adjusted hazard ratios for incident skin lesions comparing 10.1-50.0, 50.1-100.0, 100.1-200.0, and ≥200.1 μg/L with ≤10.0 μg/L of well water arsenic exposure were 1.17 (95% confidence interval (CI): 0.92, 1.49), 1.69 (95% CI: 1.33, 2.14), 1.97 (95% CI: 1.58, 2.46), and 2.98 (95% CI: 2.40, 3.71), respectively (P(trend) = 0.0001).
    explanation: Prospective dose-response data support chronic arsenical cutaneous toxicity.
- name: Hepatic biochemical injury
  biological_scale: TISSUE
  mechanism_confidence: PROVISIONAL
  subtypes:
  - Chronic
  description: >-
    Higher chronic arsenic exposure is associated with increased ALP, AST, and
    ALT activities. These cross-sectional biochemical associations do not by
    themselves establish chronic liver failure or fibrosis.
  locations:
  - preferred_term: liver
    term:
      id: UBERON:0002107
      label: liver
  evidence:
  - reference: PMID:21740555
    reference_title: 'Dose-response relationship between arsenic exposure and the serum enzymes for liver function tests in the individuals exposed to arsenic: a cross sectional study in Bangladesh.'
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Further, this study revealed a novel exposure- and dose- response relationship between arsenic exposure metrics and serum hepatic enzyme activity.
    explanation: Supports a biochemical exposure-response relationship while preserving the study's cross-sectional boundary.
phenotypes:
- name: Vomiting
  category: Acute gastrointestinal
  subtype: Acute
  description: Vomiting can occur early after a large inorganic arsenic ingestion.
  phenotype_term:
    preferred_term: Vomiting
    temporality: ACUTE
    term:
      id: HP:0002013
      label: Vomiting
  evidence:
  - reference: PMID:15533028
    reference_title: Survival after a lethal dose of arsenic trioxide.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Classical symptoms of an acute arsenicum (As) poisoning such as gastrointestinal cramps, vomiting, diarrhea, ECG changes and disturbed liver function tests were observed.
    explanation: Directly supports vomiting in acute poisoning.
- name: Diarrhea
  category: Acute gastrointestinal
  subtype: Acute
  description: Diarrhea can occur early after a large inorganic arsenic ingestion.
  phenotype_term:
    preferred_term: Diarrhea
    temporality: ACUTE
    term:
      id: HP:0002014
      label: Diarrhea
  evidence:
  - reference: PMID:15533028
    reference_title: Survival after a lethal dose of arsenic trioxide.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Classical symptoms of an acute arsenicum (As) poisoning such as gastrointestinal cramps, vomiting, diarrhea, ECG changes and disturbed liver function tests were observed.
    explanation: Directly supports diarrhea in acute poisoning.
- name: Shock
  category: Acute cardiovascular
  subtype: Acute
  severity: SEVERE
  description: Cardiovascular shock can dominate severe acute poisoning.
  phenotype_term:
    preferred_term: Shock
    temporality: ACUTE
    term:
      id: HP:0031273
      label: Shock
  evidence:
  - reference: PMID:3578979
    reference_title: Acute arsenic ingestion.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      A 21-year-old man presented in shock after ingesting 2 g of arsenic trioxide.
    explanation: Directly supports shock after severe acute ingestion.
- name: Prolonged QTc interval
  category: Acute cardiovascular
  subtype: Acute
  description: >-
    Marked QT-U prolongation can precede torsade de pointes in arsenic
    intoxication.
  phenotype_term:
    preferred_term: Prolonged QTc interval
    temporality: ACUTE
    term:
      id: HP:0005184
      label: Prolonged QTc interval
  evidence:
  - reference: PMID:1689832
    reference_title: Torsade de pointes and T-U wave alternans associated with arsenic poisoning.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Furthermore, marked prolongation of the QT-U interval and the rarely observed phenomenon of T-U wave alternans are demonstrated.
    explanation: Directly supports marked repolarization-interval prolongation.
- name: Delayed peripheral neuropathy
  category: Neurologic
  subtype: Acute
  description: >-
    Peripheral neuropathy may begin days to weeks after a single exposure and
    can remain abnormal years later.
  phenotype_term:
    preferred_term: Peripheral neuropathy
    temporality: SUBACUTE
    term:
      id: HP:0009830
      label: Peripheral neuropathy
  evidence:
  - reference: PMID:196051
    reference_title: Peripheral neuropathy following a single exposure to arsenic. Clincal course in four patients with electrophysiological and histological studies.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Four patients are described who developed a peripheral neuropathy 10 days to 3 weeks after ingestion of a single dose of arsenic.
    explanation: Directly supports the delayed peripheral neuropathy phenotype.
- name: Chronic hyperpigmentation
  category: Cutaneous
  subtype: Chronic
  description: Melanosis and increased basal pigmentation are characteristic chronic cutaneous findings.
  phenotype_term:
    preferred_term: Hyperpigmentation of the skin
    temporality: CHRONIC
    term:
      id: HP:0000953
      label: Hyperpigmentation of the skin
  evidence:
  - reference: PMID:11218669
    reference_title: Histopathology of skin lesions in chronic arsenic toxicity--grading of changes and study of proliferative markers.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Chronic arsenic toxicity (CAT) manifests predominantly as cutaneous lesions in the form of melanosis, keratosis and neoplastic changes.
    explanation: Directly supports melanosis in chronic arsenic toxicity.
- name: Arsenical palmar keratosis
  category: Cutaneous
  subtype: Chronic
  description: Keratotic lesions of the palms are a characteristic chronic cutaneous manifestation.
  phenotype_term:
    preferred_term: Palmoplantar keratoderma
    temporality: CHRONIC
    term:
      id: HP:0000982
      label: Palmoplantar keratoderma
  evidence:
  - reference: PMID:40583662
    reference_title: Comparison between Combination of Neem, Propylene Glycol and Salicylic Acid with Salicylic Acid Alone Topically in Arsenical Palmar Keratosis.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Thirty patients of moderate palmar arsenical keratosis from an arsenic-affected area at Cumilla in Bangladesh were recruited randomly based on inclusion and exclusion criteria.
    explanation: Directly documents palmar arsenical keratosis in a primary human treatment cohort.
histopathology:
- name: Hyperkeratotic and proliferative epidermal change
  subtype: Chronic
  diagnostic: true
  description: >-
    Biopsied arsenical skin lesions can show hyperkeratosis, parakeratosis,
    acanthosis, papillomatosis, rete-ridge elongation, increased basal
    pigmentation, and sometimes dysplasia; the pattern is supportive rather
    than independently pathognomonic.
  finding_term:
    preferred_term: Hyperkeratosis
    term:
      id: NCIT:C35541
      label: Hyperkeratosis
  evidence:
  - reference: PMID:11218669
    reference_title: Histopathology of skin lesions in chronic arsenic toxicity--grading of changes and study of proliferative markers.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Histological study of H/E stained sections showed--hyperkeratosis in 13, parakeratosis in 13, acanthosis in 12, papillomatosis in 24, elongation of reteridges in 21, increased basal pigmentation in 27 and dysplastic changes in 8 cases.
    explanation: Directly supports the retained histopathologic features in 42 human biopsies.
biochemical:
- name: Total and speciated urinary arsenic
  presence: INCREASED
  context: >-
    Total urinary arsenic reflects recent exposure but can rise markedly after
    seafood because of arsenobetaine. Speciation of inorganic arsenic, MMA, DMA,
    and arsenobetaine is necessary when the total is elevated; no universal
    diagnostic threshold is asserted here.
  biomarker_term:
    preferred_term: arsenic atom
    term:
      id: CHEBI:27563
      label: arsenic atom
  readouts:
  - target: Systemic inorganic arsenic exposure
    relationship: READOUT_OF
    direction: THRESHOLD_DEPENDENT
    endpoint_context: DIAGNOSTIC
    interpretation: >-
      The inorganic and methylated species support recent inorganic exposure,
      whereas arsenobetaine-dominant elevation can reflect seafood.
    evidence:
    - reference: PMID:18430299
      reference_title: 'Fast determination of arsenic species and total arsenic in urine by HPLC-ICP-MS: concentration ranges for unexposed german inhabitants and clinical case studies.'
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Two case studies underline the need for As speciation, especially when total urinary arsenic concentrations are elevated.
      explanation: Directly supports species-level interpretation of an elevated total.
  evidence:
  - reference: PMID:18430299
    reference_title: 'Fast determination of arsenic species and total arsenic in urine by HPLC-ICP-MS: concentration ranges for unexposed german inhabitants and clinical case studies.'
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      A maximum enhancement of total As from 1 up to 2,200 microg/L (2,000 microg/L for As-B) was observed after a normal fish meal.
    explanation: Demonstrates the magnitude of seafood-related confounding of total urinary arsenic.
- name: Serum ALP, AST, and ALT activities
  presence: INCREASED
  subtype: Chronic
  context: >-
    Increased activities correlate with higher chronic exposure in the cited
    cross-sectional cohort; they are nonspecific injury markers, not a diagnosis
    of arsenicosis or liver failure.
  readouts:
  - target: Hepatic biochemical injury
    relationship: CORRELATES_WITH
    direction: POSITIVE
    endpoint_context: DIAGNOSTIC
    interpretation: Higher hepatic enzyme activities track a biochemical liver-injury association in exposed populations.
    evidence:
    - reference: PMID:21740555
      reference_title: 'Dose-response relationship between arsenic exposure and the serum enzymes for liver function tests in the individuals exposed to arsenic: a cross sectional study in Bangladesh.'
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        the respective activities of ALP, AST and ALT were found to be significantly increased in the high-exposure groups compared to the lowest-exposure groups before and after adjustments were made for different covariates.
      explanation: Directly supports the exposure-stratified biochemical association.
  evidence:
  - reference: PMID:21740555
    reference_title: 'Dose-response relationship between arsenic exposure and the serum enzymes for liver function tests in the individuals exposed to arsenic: a cross sectional study in Bangladesh.'
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Elevated serum hepatic enzyme activities in the higher exposure gradients provided new insights into arsenic-induced liver toxicity that might be helpful for the early prognosis of arsenic-induced liver diseases.
    explanation: Supports increased hepatic enzyme activity as an exposure-associated biochemical finding.
genetic:
- name: AS3MT
  gene_term:
    preferred_term: AS3MT
    term:
      id: hgnc:17452
      label: AS3MT
  relationship_type: SUSCEPTIBILITY
  association: Exposure-response modifier of arsenic metabolism and arsenical skin-lesion risk
  notes: >-
    Common variation near AS3MT changes MMA and DMA proportions and modifies
    skin-lesion risk in exposed people; it is neither necessary nor sufficient
    to cause arsenic poisoning.
  evidence:
  - reference: PMID:22383894
    reference_title: Genome-wide association study identifies chromosome 10q24.32 variants associated with arsenic metabolism and toxicity phenotypes in Bangladesh.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      In a follow-up analysis of 1,085 individuals with arsenic-induced premalignant skin lesions (the classical sign of arsenic toxicity) and 1,794 controls, we show that one of these five variants (rs9527) is also associated with skin lesion risk (P = 0.0005).
    explanation: Directly supports an AS3MT-region susceptibility association under arsenic exposure.
environmental:
- name: Chronic ingestion of arsenic-contaminated drinking water
  exposure_term:
    preferred_term: exposure to arsenic in water via ingestion
    term:
      id: ECTO:0080000
      label: exposure to arsenic in water via ingestion
  description: >-
    Sustained ingestion of inorganic arsenic in groundwater is the central
    exposure context for chronic arsenicosis.
  evidence:
  - reference: PMID:21576319
    reference_title: A prospective study of arsenic exposure from drinking water and incidence of skin lesions in Bangladesh.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Chronic arsenic exposure from drinking water was associated with increased incidence of skin lesions, even at low levels of arsenic exposure (<100 μg/L).
    explanation: Prospective human data support contaminated drinking water as a chronic causal exposure.
- name: Acute high-dose inorganic arsenic ingestion
  exposure_term:
    preferred_term: exposure to arsenic
    term:
      id: ECTO:9000032
      label: exposure to arsenic
  description: >-
    Accidental or intentional ingestion of a concentrated inorganic arsenic
    compound can produce life-threatening acute poisoning.
  evidence:
  - reference: PMID:3578979
    reference_title: Acute arsenic ingestion.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      A 21-year-old man presented in shock after ingesting 2 g of arsenic trioxide.
    explanation: Directly supports concentrated inorganic arsenic ingestion as an acute exposure.
  - reference: PMID:12793928
    reference_title: 'Treatment and toxicokinetics of acute pediatric arsenic ingestion: danger of arsenic insecticides in children.'
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      A 22-month-old boy ingested approximately twice the estimated lethal dose of arsenic trioxide (As(2)O(3)) ant bait.
    explanation: Directly supports accidental pediatric ingestion of an arsenical insecticide.
treatments:
- name: Exposure cessation and arsenic-safe water
  action_category: THERAPEUTIC
  description: >-
    Remove the exposure source. For chronic drinking-water arsenicosis, provide
    reliably arsenic-safe water and prevent renewed exposure. Skin lesions can
    improve over years, but established severe disease may persist.
  treatment_term:
    preferred_term: chemical exposure avoidance
    term:
      id: NCIT:C15900
      label: Lifestyle Therapy
  target_mechanisms:
  - target: Systemic inorganic arsenic exposure
    treatment_effect: INHIBITS
    description: Preventing further ingestion reduces ongoing systemic arsenic exposure.
    evidence:
    - reference: PMID:25161968
      reference_title: 'Effect of Safe Water on Arsenicosis: A Follow-up Study.'
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        All the participants who had arsenic contaminated drinking water source in their houses were supplied with arsenic removal filters for getting arsenic-free water during the follow-up period.
      explanation: Directly documents exposure mitigation in the human follow-up study.
  evidence:
  - reference: PMID:25161968
    reference_title: 'Effect of Safe Water on Arsenicosis: A Follow-up Study.'
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      In participants belonging to Cohort-I, the skin score was found to improve significantly at the end of each year, and it was found to be reduced significantly from 2.17 ± 1.09 to 1.23 ± 1.17; P < 0.001 at the end of 3 year's intervention study indicating beneficial effect of safe water on skin lesions.
    explanation: Supports clinical improvement after provision of arsenic-safe water.
- name: Early supportive resuscitation and intensive monitoring
  action_category: THERAPEUTIC
  description: >-
    Acute severe poisoning requires immediate restoration and maintenance of
    cardiac output and renal perfusion, with continuous cardiovascular,
    electrolyte, urine-output, and acid-base monitoring.
  treatment_term:
    preferred_term: supportive care
    term:
      id: NCIT:C15747
      label: Supportive Care
  target_mechanisms:
  - target: Acute gastrointestinal and hemodynamic toxicity
    treatment_effect: RESTORES
    description: Resuscitation restores organ perfusion during early hemodynamic compromise.
    evidence:
    - reference: PMID:12793928
      reference_title: 'Treatment and toxicokinetics of acute pediatric arsenic ingestion: danger of arsenic insecticides in children.'
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Recovery from arsenic poisoning was attributable to the restoration and maintenance of adequate cardiac output and renal perfusion in early shock, which allowed depot intramuscular British anti-Lewisite to circulate and eliminate the poison.
      explanation: Directly supports early restoration of perfusion in this acute survivor.
  evidence:
  - reference: PMID:12793928
    reference_title: 'Treatment and toxicokinetics of acute pediatric arsenic ingestion: danger of arsenic insecticides in children.'
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Continuous monitoring in the pediatric intensive care unit included continuous electrocardiogram, arterial blood pressure, urine output, vital signs, arterial blood gases, serum and urine arsenic concentrations, electrolytes, electromyography, and determination of arsenic toxicokinetics.
    explanation: Directly supports the intensive monitoring components retained here.
- name: Early dithiol chelation for severe acute poisoning
  action_category: THERAPEUTIC
  therapeutic_modality: SMALL_MOLECULE
  description: >-
    Dimercaprol, succimer, or DMPS may be considered early for severe acute
    inorganic arsenic poisoning with toxicology consultation. Human evidence is
    largely multi-intervention case experience, so this entry does not rank the
    agents or assert controlled outcome benefit.
  treatment_term:
    preferred_term: chelator agent therapy
    term:
      id: NCIT:C15986
      label: Pharmacotherapy
    therapeutic_agent:
    - preferred_term: dimercaprol
      term:
        id: CHEBI:64198
        label: dimercaprol
    - preferred_term: succimer
      term:
        id: CHEBI:63623
        label: succimer
    - preferred_term: 2,3-disulfanylpropane-1-sulfonic acid
      term:
        id: CHEBI:888
        label: 2,3-disulfanylpropane-1-sulfonic acid
  target_mechanisms:
  - target: Systemic inorganic arsenic exposure
    treatment_effect: INHIBITS
    description: Dithiol ligands can bind arsenic species and promote urinary excretion.
    evidence:
    - reference: PMID:12387631
      reference_title: Determination of arsenic metabolic complex excreted in human urine after administration of sodium 2,3-dimercapto-1-propane sulfonate.
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        The finding of the DMPS-MMA(III) complex in human urine after DMPS treatment provides an explanation for the inhibition of arsenic methylation by DMPS.
      explanation: Demonstrates a human urinary DMPS-arsenic complex supporting the chelation mechanism.
  evidence:
  - reference: PMID:12793928
    reference_title: 'Treatment and toxicokinetics of acute pediatric arsenic ingestion: danger of arsenic insecticides in children.'
    supports: PARTIAL
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      He was stabilized with fluid resuscitation, placed on a sodium bicarbonate intravenous drip, and treated with intramuscular dimercaprol (British anti-Lewisite), 5 mg/kg every 6 hrs for 3 days.
    explanation: >-
      Documents early dimercaprol use in a survivor but cannot isolate chelation
      efficacy from simultaneous resuscitation and other treatment.
  - reference: PMID:15533028
    reference_title: Survival after a lethal dose of arsenic trioxide.
    supports: PARTIAL
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Forced diuresis, BAL (2,3-dimercaptopropanol) and DMSA (meso-2,3-dimercaptosuccinic acid) were started and therapy to enhance the formation of methylated As derivatives, which are potentially less toxic and which can be excreted more easily, was then administered.
    explanation: >-
      Documents BAL and DMSA within a successful multi-intervention case, not a
      controlled estimate of benefit.
diagnosis:
- name: Total urinary arsenic with species-level interpretation
  diagnosis_term:
    preferred_term: urine chemistry measurement
    term:
      id: NCIT:C61044
      label: Urine Chemistry Measurement
  description: >-
    Measure urinary arsenic after a compatible exposure and obtain speciation
    when total arsenic is elevated. Interpret inorganic species and MMA/DMA
    separately from arsenobetaine, which can increase substantially after
    seafood. A universal diagnostic cutoff is not asserted.
  evidence:
  - reference: PMID:18430299
    reference_title: 'Fast determination of arsenic species and total arsenic in urine by HPLC-ICP-MS: concentration ranges for unexposed german inhabitants and clinical case studies.'
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Two case studies underline the need for As speciation, especially when total urinary arsenic concentrations are elevated.
    explanation: Directly supports urinary speciation when total arsenic is elevated.
- name: Acute electrocardiographic and physiologic monitoring
  diagnosis_term:
    preferred_term: electrocardiography
    term:
      id: NCIT:C38053
      label: Electrocardiography
  description: >-
    In severe acute poisoning, continuously monitor electrocardiography,
    perfusion, urine output, acid-base status, and electrolytes because
    hemodynamic compromise and malignant ventricular arrhythmia can occur.
  evidence:
  - reference: PMID:12793928
    reference_title: 'Treatment and toxicokinetics of acute pediatric arsenic ingestion: danger of arsenic insecticides in children.'
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Continuous monitoring in the pediatric intensive care unit included continuous electrocardiogram, arterial blood pressure, urine output, vital signs, arterial blood gases, serum and urine arsenic concentrations, electrolytes, electromyography, and determination of arsenic toxicokinetics.
    explanation: Directly supports the retained acute monitoring strategy.
  - reference: PMID:1689832
    reference_title: Torsade de pointes and T-U wave alternans associated with arsenic poisoning.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      T-U wave alternans occurs in the presence of a long QT-U interval and may be an electrocardiographic warning sign of torsade de pointes.
    explanation: Supports ECG monitoring for repolarization warning signs.
- name: Nerve conduction study for delayed neuropathy
  diagnosis_term:
    preferred_term: nerve conduction study
    term:
      id: NCIT:C88502
      label: Nerve Conduction Velocity Test
  description: >-
    Electrophysiologic testing can document motor-conduction slowing and marked
    sensory nerve action-potential abnormalities when delayed neuropathy follows
    acute exposure.
  evidence:
  - reference: PMID:196051
    reference_title: Peripheral neuropathy following a single exposure to arsenic. Clincal course in four patients with electrophysiological and histological studies.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Electrophysiological studies showed reduction of motor conduction velocity and marked abnormalities of sensory nerve action potentials.
    explanation: Directly supports nerve-conduction testing for arsenic-associated neuropathy.
discussions:
- discussion_id: gap_arsenic_acute_chelation_outcomes
  prompt: >-
    Which dithiol chelator, timing, and regimen improve patient-centered outcomes
    in severe acute inorganic arsenic poisoning beyond modern supportive care?
  kind: KNOWLEDGE_GAP
  status: OPEN
  attaches_to:
  - treatments#Early dithiol chelation for severe acute poisoning
  rationale: >-
    Acute treatment reports combine chelation with resuscitation,
    gastrointestinal decontamination, and other interventions, preventing a
    clean estimate of comparative efficacy. Chronic arsenicosis should not be
    used as a proxy indication: a randomized placebo-controlled DMSA trial did
    not show clinical, biochemical, or histopathologic benefit.
  evidence:
  - reference: PMID:9865236
    reference_title: Randomized placebo-controlled trial of 2,3-dimercaptosuccinic acid in therapy of chronic arsenicosis due to drinking arsenic-contaminated subsoil water.
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Under the conditions of this study, 2,3-dimercaptosuccinic acid was not effective in producing any clinical or biochemical benefit or any histopathological improvement of skin lesions in patients with chronic arsenicosis.
    explanation: >-
      The randomized trial directly establishes that chronic DMSA treatment did
      not provide the asserted benefits under the studied regimen.
  - reference: PMID:15533028
    reference_title: Survival after a lethal dose of arsenic trioxide.
    supports: PARTIAL
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The patient survived this massive dose of ingested inorganic As with only polyneuropathy one year later.
    explanation: >-
      Survival after multiple simultaneous interventions motivates study but
      does not isolate chelator efficacy.
- discussion_id: gap_arsenic_molecular_human_translation
  prompt: >-
    To what extent do PDH inhibition and ROS/RNS-mediated PARP-1 impairment drive
    the acute and chronic organ syndromes in exposed humans at clinically
    relevant internal doses?
  kind: HUMAN_MODEL_MISMATCH
  status: OPEN
  attaches_to:
  - pathophysiology#MMA(III)-sensitive pyruvate dehydrogenase inhibition
  - pathophysiology#Arsenite-generated oxidative and nitrosative stress
  - pathophysiology#PARP-1 zinc loss and activity impairment
  rationale: >-
    The retained proximal mechanisms are supported by purified-enzyme, cell,
    and animal experiments, while the direct quantitative bridge to human organ
    injury remains unresolved. The graph therefore does not connect these
    molecular nodes directly to cancer, neuropathy, or cutaneous lesions.
  evidence:
  - reference: PMID:9806419
    reference_title: Arsenic toxicity is enzyme specific and its affects on ligation are not caused by the direct inhibition of DNA repair enzymes.
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      Our results show that direct enzyme inhibition is not a common toxic effect of As and that only a few sensitive enzymes are responsible for arsenic-induced cellular toxicity.
    explanation: Directly supports limiting the breadth of the enzyme-inhibition model.
  - reference: PMID:23602911
    reference_title: Arsenite-induced ROS/RNS generation causes zinc loss and inhibits the activity of poly(ADP-ribose) polymerase-1.
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: >-
      Most importantly, arsenite incubation with purified PARP-1 protein in vitro did not alter PARP-1 activity or DNA-binding ability, whereas hydrogen peroxide or NONOate retained PARP-1 inhibitory activity.
    explanation: >-
      Supports an indirect ROS/RNS-mediated cellular mechanism and the need to
      test its relevance in human tissues.
📚

References & Deep Research

Deep Research

1
Falcon
1. Disease Information
Edison Scientific Literature 29 citations 2026-05-08T09:19:48.807697

1. Disease Information

1.1 Concise overview

Arsenic poisoning refers to adverse clinical outcomes resulting from exposure to arsenic compounds, most importantly inorganic arsenic (iAs). Acute intoxication often follows ingestion of iAs and can present within ~30 minutes to 2 hours with severe gastroenteritis, hypotension, cardiac conduction abnormalities (including QT prolongation), neurologic toxicity (delirium, seizures), and acute kidney injury. (balalimood2025recentadvancesin pages 12-13)

Chronic arsenic poisoning is commonly termed arsenicosis and results from long-term low-dose exposure, typically via contaminated drinking water and/or diet. It features characteristic dermatologic findings (hyperpigmentation with “raindrop” pattern, palmoplantar hyperkeratosis), peripheral neuropathy, vascular disease, and increased risk of cancers (skin, bladder, lung, and others). (ganie2024arsenictoxicitysources pages 2-3, chakif2026heavymetaltoxicity pages 12-13)

1.2 Synonyms and alternative names

Chronic arsenic toxicity is explicitly described as arsenicosis, historically also called arseniasis, arsenism, arsenicism. (ganie2024arsenictoxicitysources pages 2-3)

1.3 Key identifiers (ontology/terminology)

  • ICD-10/ICD-11: Not found in retrieved sources.
  • MeSH: Not found in retrieved sources.
  • MONDO: Not found in retrieved sources.

1.4 Evidence provenance

The information summarized here is derived from aggregated disease-level resources (systematic reviews, regulatory assessments, narrative reviews) and mechanistic/model-system studies, rather than individual EHR extractions. (balalimood2025recentadvancesin pages 12-13, visciano2025arsenicinwater pages 10-12, issanov2023arsenicindrinking pages 1-2)

2. Etiology

2.1 Causal factors

  • Environmental/toxic exposure: Exposure to inorganic arsenic species—especially As(III) (arsenite) and As(V) (arsenate)—from groundwater, food, industrial sources, and in some circumstances inhalational exposures (including arsine gas). (balalimood2025recentadvancesin pages 12-13, ganie2024arsenictoxicitysources pages 1-2)
  • Chemical species matter: Trivalent arsenicals are emphasized as more toxic due to strong interactions with sulfur-containing proteins/thiols. (ganie2024arsenictoxicitysources pages 1-2)

2.2 Risk factors

Environmental/occupational

  • Drinking-water contamination is repeatedly highlighted as a principal route in high-burden regions (e.g., South Asia). (ganie2024arsenictoxicitysources pages 1-2)
  • Global burden: An opinion/global scenario paper reports groundwater arsenic contamination affecting 106 countries and exposing ~230 million people (ATSDR 2022 ranking and WHO “top 10 chemicals” framing also noted). (bhat2024arseniccontaminationneeds pages 1-2)

Host and contextual risk modifiers

  • Genetic susceptibility: Inter-individual differences in arsenic metabolism (methylation phenotype) are linked to susceptibility; methylation depends on AS3MT and related pathways. (ganie2024arsenictoxicitysources pages 2-3, pullella2024elucidatingtherelationship pages 37-41)
  • Nutritional status / one‑carbon metabolism: Methyl-donor availability (folate and related nutrients) modifies arsenic methylation capacity and may influence toxicity. (pereira2025arsenomearsenobolomeand pages 20-22, abuawad2023thefolicacid pages 7-8)

2.3 Protective factors

  • Nutritional supplementation improving methylation indices (human evidence): In the FACT trial (Bangladesh; randomized, double-blind, placebo-controlled), folic acid supplementation improved blood arsenic methylation indices and metabolite profiles (increased SMI and %DMAs, decreased %MMAs), suggesting improved detoxification via enhanced methylation. (abuawad2023thefolicacid pages 7-8, abuawad2023thefolicacid pages 1-2)

2.4 Gene–environment interactions

  • Mechanistic G×E framing: Variation in response to arsenic metabolites is genetically regulated in model systems; QTL mapping under MMA(III) exposure implicated detoxification and DNA repair loci (e.g., Abcc4, Txnrd1, Xrcc2), supporting gene-by-environment modulation of oxidative stress response and cell death trajectories. (o’connor2024unravelingthegenetics pages 1-2)

3. Phenotypes (clinical manifestations)

A structured phenotype-to-HPO mapping is provided in Artifact-01.

Clinical feature Acute/Chronic Description/onset notes Suggested HPO term(s) Evidence (citation IDs)
Nausea and vomiting Acute Common early gastrointestinal manifestations; acute symptoms may begin within ~30 minutes to 2 hours after ingestion Nausea (HP:0002018); Vomiting (HP:0002013) (balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13)
Diarrhea Acute Prominent early gastroenteritis in acute inorganic arsenic ingestion Diarrhea (HP:0002014) (balalimood2025recentadvancesin pages 12-13, balalimood2025recentadvancesin pages 13-14)
Abdominal pain Acute Early abdominal pain/cramping as part of acute gastroenteritis syndrome Abdominal pain (HP:0002027) (balalimood2025recentadvancesin pages 12-13)
Dehydration / hypovolemia Acute Follows severe vomiting and diarrhea; contributes to shock and mortality Dehydration (HP:0001944); Hypovolemia (balalimood2025recentadvancesin pages 12-13, balalimood2025recentadvancesin pages 13-14)
Hypotension Acute Reported in severe poisoning, often secondary to fluid loss and systemic toxicity Hypotension (HP:0002615) (balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13)
QT prolongation / arrhythmia Acute ECG abnormalities include prolonged QT and other conduction disturbances; can progress to torsades/serious arrhythmia Prolonged QT interval (HP:0005184); Cardiac arrhythmia (HP:0011675) (balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13, pereira2025arsenomearsenobolomeand pages 19-20)
Tremor Acute Neurologic sign reported in acute intoxication Tremor (HP:0001337) (balalimood2025recentadvancesin pages 12-13)
Delirium / encephalopathy Acute Severe neurotoxicity may include delirium and central nervous system dysfunction Delirium (HP:0031258); Encephalopathy (HP:0001298) (balalimood2025recentadvancesin pages 12-13, pereira2025arsenomearsenobolomeand pages 19-20)
Seizures Acute Can occur in severe poisoning as part of CNS involvement Seizure (HP:0001250) (balalimood2025recentadvancesin pages 12-13, balalimood2025recentadvancesin pages 13-14)
Proteinuria / hematuria Acute Renal involvement in acute poisoning may include urinary abnormalities and acute tubular injury Proteinuria (HP:0000093); Hematuria (HP:0000790) (balalimood2025recentadvancesin pages 12-13)
Acute kidney injury Acute Severe poisoning may cause acute tubular necrosis/renal failure Acute kidney injury (HP:0031270) (balalimood2025recentadvancesin pages 12-13, balalimood2025recentadvancesin pages 13-14)
Peripheral neuropathy Subacute/Chronic Characteristic sensory > motor neuropathy; may appear 2-4 weeks after acute exposure or develop with chronic exposure Peripheral neuropathy (HP:0009830); Sensory neuropathy (HP:0000763); Motor neuropathy (balalimood2025recentadvancesin pages 12-13, ganie2024arsenictoxicitysources pages 2-3, chakif2026heavymetaltoxicity pages 12-13, pereira2025arsenomearsenobolomeand pages 20-22)
Hyperpigmentation Chronic Classic skin manifestation, often diffuse or spotted; chronic arsenicosis hallmark Hyperpigmentation of the skin (HP:0000953) (balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13, pereira2025arsenomearsenobolomeand pages 20-22)
Raindrop-pattern pigmentation Chronic Characteristic mottled hyper/hypopigmented skin change in chronic arsenic toxicity Mottled pigmentation (balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13)
Palmoplantar hyperkeratosis Chronic Typical chronic dermal lesion; affects palms and soles Palmoplantar hyperkeratosis (HP:0000982) (balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13, pereira2025arsenomearsenobolomeand pages 20-22)
Desquamation Chronic Reported with chronic arsenic-related dermal injury Desquamation (HP:0001029) (pereira2025arsenomearsenobolomeand pages 20-22)
Mees lines Chronic Transverse white nail bands associated with chronic exposure Leukonychia striata / Mees lines (balalimood2025recentadvancesin pages 12-13)
Cognitive / attention impairment Chronic Chronic exposure linked to learning, memory, and attention deficits Cognitive impairment (HP:0100543); Abnormality of attention (ganie2024arsenictoxicitysources pages 2-3)
Hypertension / vascular disease Chronic Chronic exposure associated with peripheral vascular disease and hypertension; Blackfoot disease is a classic severe vascular manifestation Hypertension (HP:0000822); Peripheral vascular disease; Blackfoot disease (ganie2024arsenictoxicitysources pages 1-2, pereira2025arsenomearsenobolomeand pages 20-22)
Skin cancer Chronic complication Chronic inorganic arsenic exposure increases skin cancer risk Skin neoplasm (HP:0012126) (balalimood2025recentadvancesin pages 12-13, ganie2024arsenictoxicitysources pages 1-2)
Bladder cancer Chronic complication Strong epidemiologic association with long-term exposure Bladder neoplasm (HP:0100747) (chakif2026heavymetaltoxicity pages 12-13, pereira2025arsenomearsenobolomeand pages 20-22)
Lung cancer Chronic complication Established chronic carcinogenic outcome of inorganic arsenic exposure Lung neoplasm (HP:0100526) (chakif2026heavymetaltoxicity pages 12-13, ganie2024arsenictoxicitysources pages 1-2, pereira2025arsenomearsenobolomeand pages 20-22)
Kidney or liver cancer Chronic complication Reported among internal malignancies linked to chronic exposure Renal neoplasm; Hepatic neoplasm (ganie2024arsenictoxicitysources pages 1-2, pereira2025arsenomearsenobolomeand pages 20-22)

Table: This table maps major acute and chronic clinical manifestations of arsenic poisoning to suggested HPO terms for knowledge-base annotation. It emphasizes timing, characteristic arsenicosis features, and long-term cancer complications supported by recent review evidence.

Key time-course features: - Acute: Symptoms begin ~30 min–2 h after ingestion (GI symptoms prominent) with possible cardiovascular collapse and multi-organ injury. (balalimood2025recentadvancesin pages 12-13) - Subacute neurologic: Sensory deficits may appear 2–4 weeks post-exposure. (balalimood2025recentadvancesin pages 12-13) - Chronic: Dermatologic and neurologic manifestations plus long-term cancer risks. (balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13)

4. Genetic/Molecular Information

4.1 Causal genes

Arsenic poisoning is not a Mendelian genetic disease; however, genes that govern arsenic metabolism and response modify susceptibility. Strongly implicated genes/pathways include: - AS3MT (arsenic methyltransferase) for methylation of iAs to methylated metabolites. (ganie2024arsenictoxicitysources pages 2-3, singh2024arsenicexposurein pages 1-5) - Detoxification/transport and oxidative stress response genes highlighted in genetic mapping screens under MMA(III) exposure (Abcc4, Txnrd1) and DNA repair candidates (Xrcc2). (o’connor2024unravelingthegenetics pages 1-2)

4.2 Pathogenic variants

Specific human variant pathogenicity classifications (ACMG/ClinVar) were not present in retrieved sources.

4.3 Epigenetic information

Chronic iAs exposure is described as producing epigenetic alterations, including promoter hypermethylation (e.g., MLH1/MSH2), altered DNMT expression (↑DNMT1/DNMT3B, ↓DNMT3A), and global methylation changes linked to SAM depletion. (pereira2025arsenomearsenobolomeand pages 20-22, pullella2024elucidatingtherelationship pages 37-41)

5. Environmental Information

5.1 Environmental factors

  • Major exposure is via contaminated groundwater and diet; mining/manufacturing and geogenic sources are emphasized in recent reviews. (ganie2024arsenictoxicitysources pages 1-2)

5.2 Lifestyle factors

  • Dietary contributions can be substantial; risk assessments distinguish organic arsenic from seafood versus inorganic arsenic from water/food sources, requiring speciation for interpretation. (chakif2026heavymetaltoxicity pages 12-13, pullella2024elucidatingtherelationship pages 37-41)

6. Mechanism / Pathophysiology

A structured mechanism table with ontology mapping is provided in Artifact-03.

Mechanism (high level) Molecular details/chain Example genes/proteins Suggested GO biological process terms Suggested CL cell types Suggested UBERON organs/tissues Suggested CHEBI entities/arsenic species Evidence (citation IDs)
Toxicokinetic uptake, biotransformation, and distribution Ingested/inhaled inorganic arsenic is absorbed, distributed systemically, and methylated mainly in liver to MMA and DMA; As(V) enters via phosphate transporters, As(III) via aquaglyceroporins, then binds thiols and undergoes reduction/methylation using GSH, thioredoxin systems, SAM, and AS3MT. Urinary excretion is the main clearance route; skin, hair, nails, bone, and teeth can accumulate arsenic. AS3MT, TXNRD1, thioredoxin, glutathione-related proteins, aquaglyceroporins, phosphate transporters GO:0006730 one-carbon metabolic process; GO:0017144 drug metabolic process; GO:0042493 response to drug; GO:0055085 transmembrane transport hepatocyte; erythrocyte; renal tubular epithelial cell liver (UBERON:0002107); kidney (UBERON:0002113); skin (UBERON:0002097); blood (UBERON:0000178) inorganic arsenic; arsenite(3+) / As(III); arsenate(V) / As(V); monomethylarsonous acid (MMAIII); dimethylarsinic acid (DMA) (ganie2024arsenictoxicitysources pages 2-3, chakif2026heavymetaltoxicity pages 12-13, ganie2024arsenictoxicitysources pages 1-2, pullella2024elucidatingtherelationship pages 37-41)
Thiol binding and enzyme inhibition Trivalent arsenicals bind sulfhydryl/lipoic-acid–dependent enzymes, disrupting central metabolism and redox homeostasis. MMA(III) binds lipoic acid and inhibits pyruvate dehydrogenase; As(V) can substitute for phosphate in metabolic intermediates, impairing ATP-generating reactions. pyruvate dehydrogenase complex, lipoic acid–dependent enzymes, glyceraldehyde-3-phosphate dehydrogenase GO:0006099 tricarboxylic acid cycle; GO:0006096 glycolytic process; GO:0046034 ATP metabolic process; GO:0055114 oxidation-reduction process hepatocyte; cardiomyocyte; neuron liver (UBERON:0002107); heart (UBERON:0000948); nervous system (UBERON:0001016) arsenite(3+); arsenate(V); MMAIII; ADP-arsenate; glucose-6-arsenate (pereira2025arsenomearsenobolomeand pages 19-20, ganie2024arsenictoxicitysources pages 2-3, chakif2026heavymetaltoxicity pages 12-13)
Oxidative and nitrosative stress A central initiating event is ROS/RNS generation with lipid, protein, and DNA oxidation; reported biomarkers include MDA, protein carbonyls, and 8-hydroxydeoxyguanosine. MMA(III) and As(III) also interfere with nitric oxide biology, including NOS inhibition and peroxynitrite-related injury. TXNRD1, Nrf2 pathway components, nitric oxide synthase, heme oxygenase, ferritin, metallothionein GO:0006979 response to oxidative stress; GO:1903409 reactive oxygen species metabolic process; GO:0051409 response to nitrosative stress; GO:0034599 cellular response to oxidative stress fibroblast; endothelial cell; keratinocyte; neuron skin (UBERON:0002097); vasculature (UBERON:0004535); lung (UBERON:0002048); kidney (UBERON:0002113) arsenite(3+); MMAIII; inorganic arsenic (balalimood2025recentadvancesin pages 12-13, pereira2025arsenomearsenobolomeand pages 19-20, pereira2025arsenomearsenobolomeand pages 20-22, pullella2024elucidatingtherelationship pages 37-41, ganie2024arsenictoxicitysources pages 2-3)
NRF2-mediated antioxidant response and detoxification Genetic and experimental studies identify NRF2-linked antioxidant defense, detoxification, and stress-response programs as major regulated responses to arsenic metabolites. Cellular morphology QTL mapped loci including Abcc4 and Txnrd1, supporting genetically controlled variability in response to MMAIII. ABCC4, TXNRD1, NRF2 pathway genes, metallothioneins GO:0034599 cellular response to oxidative stress; GO:0042744 hydrogen peroxide catabolic process; GO:0006805 xenobiotic metabolic process; GO:0046677 response to antibiotic fibroblast; hepatocyte; renal epithelial cell liver (UBERON:0002107); kidney (UBERON:0002113); skin (UBERON:0002097) MMAIII; inorganic arsenic; arsenite(3+) (pullella2024elucidatingtherelationship pages 37-41, ganie2024arsenictoxicitysources pages 2-3)
DNA damage and impaired DNA repair Arsenic increases chromosomal abnormalities, sister chromatid exchange, oxidative DNA damage, and genomic instability. It inhibits DNA mismatch repair and broader DNA repair responses; cmQTL work highlighted DNA repair candidate Xrcc2. Chronic exposure is linked to 8-oxo-dG elevation and repair gene dysregulation. XRCC2, MLH1, MSH2, p53-related pathways GO:0006281 DNA repair; GO:0006974 cellular response to DNA damage stimulus; GO:0036297 interstrand cross-link repair; GO:0006302 double-strand break repair fibroblast; keratinocyte; urothelial cell skin (UBERON:0002097); urinary bladder (UBERON:0001255); lung (UBERON:0002048) inorganic arsenic; arsenite(3+); MMAIII (pereira2025arsenomearsenobolomeand pages 19-20, pereira2025arsenomearsenobolomeand pages 20-22, pullella2024elucidatingtherelationship pages 37-41, ganie2024arsenictoxicitysources pages 2-3)
Epigenetic dysregulation and methyl-donor depletion Arsenic perturbs epigenetic control through SAM depletion, global hypomethylation, locus-specific hypermethylation (e.g., MLH1, MSH2), altered DNMT expression, mitochondrial D-loop hypomethylation, miRNA changes, and m6A-related signaling. Nutritional methyl-donor status (folate, choline, methionine, betaine, B vitamins) modifies toxicity. DNMT1, DNMT3A, DNMT3B, MLH1, MSH2, METTL3, YTHDF2, JAK2, STAT3, AS3MT GO:0006306 DNA methylation; GO:0016573 histone acetylation; GO:0032776 DNA methylation on cytosine; GO:0010608 post-transcriptional regulation of gene expression keratinocyte; hepatocyte; stem/progenitor-like epithelial cell skin (UBERON:0002097); liver (UBERON:0002107); urinary bladder (UBERON:0001255) inorganic arsenic; arsenite(3+); methylated arsenicals (pereira2025arsenomearsenobolomeand pages 20-22, pereira2025arsenomearsenobolomeand pages 19-20, pullella2024elucidatingtherelationship pages 37-41, abuawad2023thefolicacid pages 6-7, abuawad2023thefolicacid pages 7-8, abuawad2023thefolicacid pages 1-2)
Mitochondrial dysfunction and apoptosis Arsenic disrupts mitochondrial respiration and oxidative phosphorylation, lowers ATP production, activates JNK/ERK and GRP78/CHOP stress pathways, and promotes apoptosis/cell death trajectories. These events link upstream redox injury to organ dysfunction and neuro/cardiotoxicity. JNK, ERK, GRP78, CHOP, pyruvate dehydrogenase complex GO:0007005 mitochondrion organization; GO:0008635 activation of apoptotic process; GO:1902600 proton transmembrane transport; GO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress neuron; cardiomyocyte; hepatocyte brain (UBERON:0000955); heart (UBERON:0000948); liver (UBERON:0002107) MMAIII; arsenite(3+); inorganic arsenic (pereira2025arsenomearsenobolomeand pages 19-20, ganie2024arsenictoxicitysources pages 2-3, chakif2026heavymetaltoxicity pages 12-13)
Inflammation, angiogenesis, and malignant transformation Chronic exposure activates inflammatory signaling (including NF-kB-related and CD14-linked profiles), VEGF/angiogenesis, EMT-like programs, and altered signal transduction, helping connect long-term exposure to skin, bladder, lung, kidney, and liver cancers. NFkB, VEGF, EGFR, CD14, p38 MAPK pathway components GO:0006954 inflammatory response; GO:0001525 angiogenesis; GO:0001837 epithelial to mesenchymal transition; GO:0008284 positive regulation of cell population proliferation endothelial cell; macrophage/monocyte; keratinocyte; urothelial cell skin (UBERON:0002097); lung (UBERON:0002048); urinary bladder (UBERON:0001255); kidney (UBERON:0002113) inorganic arsenic; arsenite(3+); methylated arsenicals (pereira2025arsenomearsenobolomeand pages 20-22, pullella2024elucidatingtherelationship pages 37-41, chakif2026heavymetaltoxicity pages 12-13, ganie2024arsenictoxicitysources pages 1-2)
Electrophysiologic cardiotoxicity Arsenic blocks repolarizing potassium currents (IKr, IKs), prolonging QT and predisposing to torsades/arrhythmias; this is a key downstream mechanism in acute severe poisoning. IKr channel, IKs channel GO:0086001 cardiac muscle cell action potential; GO:1903779 regulation of cardiac conduction; GO:0006813 potassium ion transport cardiomyocyte heart (UBERON:0000948) arsenite(3+); inorganic arsenic (pereira2025arsenomearsenobolomeand pages 19-20, balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13)
Population genetic susceptibility and gene-by-environment interaction Toxic response varies with arsenic methylation phenotype and genetic background. Human and model-system evidence implicates AS3MT genotype in methylation/toxicity variability, while cell-based QTL mapping identified response loci including Abcc4, Txnrd1, and Xrcc2 under MMAIII exposure. AS3MT, ABCC4, TXNRD1, XRCC2 GO:0006805 xenobiotic metabolic process; GO:0042221 response to chemical; GO:0006974 cellular response to DNA damage stimulus fibroblast; hepatocyte liver (UBERON:0002107); skin (UBERON:0002097); kidney (UBERON:0002113) MMAIII; inorganic arsenic; arsenite(3+) (ganie2024arsenictoxicitysources pages 2-3, pullella2024elucidatingtherelationship pages 37-41, abuawad2023thefolicacid pages 8-9, abuawad2023thefolicacid pages 1-2)

Table: This table summarizes major molecular and cellular mechanisms of arsenic poisoning and links them to suggested ontology terms for knowledge-base curation. It integrates toxicokinetics, oxidative stress, DNA damage, epigenetic dysregulation, mitochondrial injury, carcinogenic signaling, and genetic susceptibility.

6.1 Current mechanistic understanding (high-level causal chain)

  1. Exposure and uptake (water/food/air) → systemic distribution and hepatic biotransformation to MMA/DMA. (ganie2024arsenictoxicitysources pages 2-3, chakif2026heavymetaltoxicity pages 12-13)
  2. Molecular initiating events: thiol binding and inhibition of lipoic-acid–dependent enzymes; phosphate mimicry by arsenate; redox disruption. (pereira2025arsenomearsenobolomeand pages 19-20)
  3. Cellular injury pathways: oxidative/nitrosative stress, mitochondrial dysfunction, ER stress, apoptosis, impaired DNA repair, and epigenetic dysregulation. (pereira2025arsenomearsenobolomeand pages 19-20, pereira2025arsenomearsenobolomeand pages 20-22)
  4. Organ-level outcomes: GI injury/shock in acute poisoning; skin lesions/neuropathy/vascular disease and carcinogenesis in chronic exposure. (balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13)

7. Anatomical Structures Affected

  • Acute toxicity: gastrointestinal tract, cardiovascular system (cardiac conduction), kidney (acute tubular necrosis/AKI), liver, nervous system. (balalimood2025recentadvancesin pages 12-13)
  • Chronic toxicity: skin (hyperpigmentation, hyperkeratosis), peripheral nerves, vasculature (e.g., Blackfoot disease), and carcinogenesis in bladder, lung, and skin. (chakif2026heavymetaltoxicity pages 12-13, pereira2025arsenomearsenobolomeand pages 20-22)

Suggested UBERON targets are included in Artifact-03.

8. Temporal Development

  • Acute onset: minutes to hours after ingestion (30 min–2 h), with death possible within days if severe. (balalimood2025recentadvancesin pages 12-13)
  • Subacute stage: neuropathy may develop 2–4 weeks after exposure. (balalimood2025recentadvancesin pages 12-13)
  • Chronic course: prolonged exposure leads to arsenicosis; recovery can be prolonged and incomplete, particularly neuropathy. (balalimood2025recentadvancesin pages 13-14)

9. Inheritance and Population

9.1 Epidemiology and exposure burden

  • A global scenario/opinion report estimates arsenic-contaminated groundwater affects ~106 countries and exposes ~230 million people; WHO, EPA, and EU drinking-water limits are cited as 10 µg/L. (bhat2024arseniccontaminationneeds pages 1-2)

9.2 Regulatory coverage and inequity

  • WHO drinking-water guideline value for arsenic is 10 µg/L (0.01 mg/L) and is explicitly labeled “Provisional”, with rationale including treatment performance and analytical achievability rather than purely health-based derivation. (mitchell2023acomprehensivesurvey pages 6-8)
  • An international survey reports that 32% of the world’s population live in countries where the national arsenic standard is less protective than the WHO GV of 10 µg/L. (mitchell2023acomprehensivesurvey pages 2-4)

10. Diagnostics

A structured diagnostics table is provided in Artifact-02.

Category Item What it indicates/when used Key quantitative thresholds or notes Evidence
Diagnostic Urine total arsenic (24-hour) Main biomarker for recent arsenic exposure; used in suspected acute or ongoing exposure 24-hour urinary arsenic >100 µg/L reported as elevated/toxic in retrieved evidence; chelation follow-up target <50 µg/L in 24-hour urine; seafood can confound total urinary arsenic unless speciation is done (pullella2024elucidatingtherelationship pages 37-41, balalimood2025recentadvancesin pages 13-14, pullella2024elucidatingtherelationship pages 41-44) (pullella2024elucidatingtherelationship pages 37-41, balalimood2025recentadvancesin pages 13-14, pullella2024elucidatingtherelationship pages 41-44)
Diagnostic Spot urine arsenic Practical alternative to 24-hour collection for recent exposure assessment Spot urine >50 µg/L reported as elevated in retrieved evidence; should be interpreted with hydration correction (e.g., creatinine adjustment) and ideally with speciation (balalimood2025recentadvancesin pages 12-13, pullella2024elucidatingtherelationship pages 37-41, pullella2024elucidatingtherelationship pages 41-44) (balalimood2025recentadvancesin pages 12-13, pullella2024elucidatingtherelationship pages 37-41, pullella2024elucidatingtherelationship pages 41-44)
Diagnostic Urine arsenic speciation (iAs, MMA, DMA) Best test to distinguish toxic inorganic exposure from seafood-derived organic arsenic; also used to assess methylation phenotype and susceptibility Requires seafood avoidance before testing; elevated urinary MMA or higher MMA/DMA ratio suggests less complete methylation and potentially higher cancer susceptibility; recent exposure window roughly several days because biologic half-life is about 2-4 days (chakif2026heavymetaltoxicity pages 12-13, pullella2024elucidatingtherelationship pages 37-41) (chakif2026heavymetaltoxicity pages 12-13, pullella2024elucidatingtherelationship pages 37-41)
Diagnostic Blood arsenic Reflects very recent exposure and acute poisoning; less useful after rapid clearance Blood arsenic >130 nmol/L reported as elevated/toxic in retrieved evidence; blood half-life about 2-6 h, so sensitivity falls quickly after exposure (pullella2024elucidatingtherelationship pages 37-41, pullella2024elucidatingtherelationship pages 41-44) (pullella2024elucidatingtherelationship pages 37-41, pullella2024elucidatingtherelationship pages 41-44)
Diagnostic Serum arsenic Limited clinical utility because arsenic clears rapidly from blood Not considered reliable for diagnosis once time has elapsed after exposure (balalimood2025recentadvancesin pages 12-13) (balalimood2025recentadvancesin pages 12-13)
Diagnostic Hair arsenic Marker of longer-term past exposure Can become positive about 30 h after exposure; reflects longer-term exposure but is nondiscriminatory for source/species and is not ideal for acute decision-making (balalimood2025recentadvancesin pages 12-13, pullella2024elucidatingtherelationship pages 37-41) (balalimood2025recentadvancesin pages 12-13, pullella2024elucidatingtherelationship pages 37-41)
Diagnostic Nail arsenic (especially toenail) Marker of chronic exposure over prior months Toenail arsenic >0.5 µg/g reported as elevated in retrieved evidence; nails reflect long-term exposure over about 3-6 months (pullella2024elucidatingtherelationship pages 37-41, pullella2024elucidatingtherelationship pages 41-44) (pullella2024elucidatingtherelationship pages 37-41, pullella2024elucidatingtherelationship pages 41-44)
Diagnostic Imaging (abdominal/chest X-ray) Supportive test in acute poisoning to identify radiopaque ingested arsenic material or evaluate complications Used selectively in acute ingestion; not a biomarker of body burden (balalimood2025recentadvancesin pages 12-13) (balalimood2025recentadvancesin pages 12-13)
Diagnostic ECG monitoring Detects cardiotoxicity in acute poisoning Important because acute arsenic can prolong QT/QRS and trigger torsades/arrhythmias (balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13) (balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13)
Treatment Exposure cessation/source removal First-line intervention in all cases, especially chronic/subacute arsenicosis Removal from contaminated water/food/occupational source is the primary treatment for chronic poisoning (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment Succimer / DMSA Oral chelator used for arsenic poisoning, especially when prolonged treatment is needed or less invasive therapy is preferred Named as a key chelator; preferred for prolonged chronic/subacute cases in retrieved evidence; most effective when started minutes to hours after exposure (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment DMPS Water-soluble chelator used in arsenic poisoning Named as a key arsenic chelator; most useful early after exposure; use varies by region/regulatory approval (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment Dimercaprol / BAL Traditional parenteral chelator for severe acute arsenic poisoning Named as a key chelator; most effective when given soon after exposure, typically minutes to hours (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment IV fluids and electrolyte replacement Core supportive therapy for acute poisoning with severe vomiting/diarrhea and shock Critical because deaths often result from hypovolemia, renal failure, or cardiac complications (balalimood2025recentadvancesin pages 12-13, balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 12-13, balalimood2025recentadvancesin pages 13-14)
Treatment GI decontamination / bowel irrigation / NG suction Used in selected acute ingestions, especially if arsenic is still in the GI tract or radiopaque material is seen Activated charcoal adsorbs arsenic poorly; whole-bowel irrigation or continued NG suction may be considered in severe ingestion (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment Antiarrhythmic management and magnesium Supportive management for QT prolongation/torsades and other arrhythmias Magnesium sulfate, amiodarone, or lidocaine reported as options; avoid class IA/IC/III antiarrhythmics in this context per retrieved review (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment Benzodiazepines for seizures Symptom-directed treatment in acute neurotoxicity Used when seizures occur during severe intoxication (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment Hemodialysis Adjunctive/supportive therapy in severe poisoning with kidney failure or oliguria Recommended in acute kidney injury/oliguria; supportive rather than stand-alone antidotal therapy (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment Exchange transfusion Special situation therapy for arsine gas poisoning with massive hemolysis Can help remove arsine-related toxic burden in severe hemolytic presentations (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment Antioxidant/nutritional adjuncts Investigational or supportive approaches to mitigate toxicity or improve methylation/detoxification Selenium, zinc, folate, vitamins A/C/E, and phytochemicals have been proposed; clinical evidence remains limited (balalimood2025recentadvancesin pages 13-14, abuawad2023thefolicacid pages 6-7, abuawad2023thefolicacid pages 7-8, abuawad2023thefolicacid pages 1-2) (balalimood2025recentadvancesin pages 13-14, abuawad2023thefolicacid pages 6-7, abuawad2023thefolicacid pages 7-8, abuawad2023thefolicacid pages 1-2)

Table: This table summarizes clinically relevant diagnostic biomarkers and current treatment approaches for arsenic poisoning, including practical interpretation notes and quantitative thresholds reported in the retrieved evidence. It is useful for distinguishing recent versus chronic exposure and for mapping acute management options to the evidence base.

Key points: - Urine arsenic speciation (iAs/MMA/DMA) is emphasized as essential to distinguish inorganic exposure from seafood-related organic arsenic and to evaluate methylation phenotype. (chakif2026heavymetaltoxicity pages 12-13, pullella2024elucidatingtherelationship pages 37-41) - Quantitative cutoffs reported in retrieved sources include blood arsenic >130 nmol/L, 24‑h urinary total arsenic >100 µg/L, spot urine >50 µg/L, and toenail As >0.5 µg/g (noting these are presented in a review context and should be interpreted clinically). (pullella2024elucidatingtherelationship pages 41-44)

11. Outcome / Prognosis

11.1 Acute poisoning outcomes

  • Minimal lethal dose reported as 1–3 mg/kg, with an estimated lethal inorganic exposure around 0.6 mg/kg; death may occur within 1–4 days after ingestion. (balalimood2025recentadvancesin pages 12-13)
  • Arsine gas exposures above ~10 ppm are described as lethal. (balalimood2025recentadvancesin pages 12-13)

11.2 Long-term complications and quantitative cancer risks

  • A 2023 systematic review/meta-analysis update reported Bayesian pooled RRs for bladder cancer incidence of 1.25 at 10 µg/L, 2.11 at 50 µg/L, and 3.01 at 150 µg/L; and for kidney cancer of 1.37 at 10 µg/L, 1.95 at 50 µg/L, and 2.47 at 150 µg/L. (issanov2023arsenicindrinking pages 1-2)

12. Treatment

12.1 Acute management (real-world implementation)

  • Core management includes aggressive IV fluids/electrolytes, symptom control (antiemetics), arrhythmia and seizure management, and renal support including hemodialysis for AKI/oliguria. (balalimood2025recentadvancesin pages 13-14)
  • Chelation: key agents named include succimer (DMSA), DMPS, and dimercaprol (BAL); chelators are described as most effective minutes to hours after exposure. A suggested chelation target is 24‑h urinary arsenic <50 µg/L. (balalimood2025recentadvancesin pages 13-14)
  • Chronic/subacute arsenicosis: exposure cessation is primary, chelation has limited efficacy, and succimer is noted as preferred for prolonged cases with monitoring. (balalimood2025recentadvancesin pages 13-14)

12.2 Nutritional intervention evidence (human trial)

  • FACT trial (NCT01050556; Bangladesh; 2023 EHP): folic acid (400–800 µg/day) improved blood methylation profiles (increased SMI and %DMAs, decreased %MMAs), with partial reversal upon stopping supplementation, supporting sustained methyl-donor strategies (e.g., fortification) as a plausible mitigation adjunct. (abuawad2023thefolicacid pages 1-2, abuawad2023thefolicacid pages 7-8)

Suggested MAXO terms (names only): chelation therapy; hemodialysis; gastrointestinal decontamination; nutritional supplementation; exposure avoidance/remediation.

13. Prevention

  • Primary prevention: reduce iAs exposure through enforcement of drinking-water standards and mitigation/removal technologies; WHO GV is 10 µg/L (provisional) and set with feasibility constraints. (mitchell2023acomprehensivesurvey pages 6-8)
  • Population-scale mitigation urgency: global scenario evidence supports the large exposed population and multi-country distribution, motivating monitoring and remediation prioritization. (bhat2024arseniccontaminationneeds pages 1-2)

14. Other Species / Natural Disease

The retrieved sources did not provide well-documented naturally occurring “arsenicosis” case series in companion animals; however, the toxicant is relevant across species and arsenic exposure is discussed in livestock contexts in broader heavy-metal reviews (not specific to arsenic-only disease characterization).

15. Model Organisms

  • Humanized AS3MT mouse model (C57BL/6): A 2024 study used C57BL/6 mice carrying the human BORCS7/AS3MT locus; with 200 ppb arsenite exposures during different developmental windows, the model showed sex- and window-specific increases in fasting glycemia and impaired β-cell function (lower HOMA‑β in in utero exposed males), and it is presented as more human-relevant because standard mice methylate/detoxify iAs more efficiently than humans. (singh2024arsenicexposurein pages 1-5)
  • Genetically diverse in vitro model (Diversity Outbred fibroblasts): A 2024 PLOS Genetics study derived fibroblast lines from DO mice and exposed them to MMA(III), using high-content imaging to map cell morphology QTLs; loci included known detox genes (Abcc4, Txnrd1) and DNA repair (Xrcc2), enabling gene–environment mapping of arsenic sensitivity/resilience. (o’connor2024unravelingthegenetics pages 2-3, o’connor2024unravelingthegenetics pages 1-2)

Recent developments and expert analysis (2023–2024 emphasis)

  • Regulatory science update: WHO’s arsenic GV remains 10 µg/L (provisional) and is explicitly constrained by treatment/analytical feasibility, highlighting a persistent gap between health-based values and implementable standards. (mitchell2023acomprehensivesurvey pages 6-8)
  • Quantitative cancer risk at low-to-moderate exposures: Updated systematic review evidence supports elevated bladder and kidney cancer risk even at 10 µg/L, albeit with uncertainty, with increasing risks at 50 and 150 µg/L. (issanov2023arsenicindrinking pages 1-2)
  • Global public-health framing (expert opinion): Recent global scenario/opinion work emphasizes urgent mitigation given widespread exposure (230 million) and broad geographic footprint (106 countries). (bhat2024arseniccontaminationneeds pages 1-2)

Key concepts and definitions (summary tables)

Concept Definition/notes Synonyms Key exposure route(s) Identifier(s) explicitly available in retrieved evidence Key citation ID
Acute arsenic poisoning Rapid-onset toxicity, usually after ingestion of inorganic arsenic; symptoms may begin within ~30 min to 2 h and commonly include severe gastroenteritis, hypotension, QT prolongation/arrhythmia, neurologic toxicity, renal injury, and hepatic/hematologic abnormalities. Acute arsenic toxicity Ingestion; less commonly inhalation in occupational settings Not found in retrieved sources (balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13)
Chronic arsenic poisoning / arsenicosis Slow accumulation of low-dose exposure over time causing multisystem disease, especially skin lesions, peripheral neuropathy, vascular disease, and elevated cancer risk; explicitly named “arsenicosis.” Arsenicosis; arseniasis; arsenism; arsenicism Chronic ingestion via drinking water/food; inhalation in some occupational settings Not found in retrieved sources (ganie2024arsenictoxicitysources pages 2-3, ganie2024arsenictoxicitysources pages 1-2, pereira2025arsenomearsenobolomeand pages 20-22)
Inorganic arsenic (iAs) Toxicologically most important arsenic category; includes pentavalent arsenate and trivalent arsenite, undergoes hepatic methylation to MMA and DMA, and is associated with carcinogenic, vascular, neurologic, and dermatologic effects. iAs; inorganic As Ingestion from contaminated water/food; inhalation Not found in retrieved sources (ganie2024arsenictoxicitysources pages 2-3, chakif2026heavymetaltoxicity pages 12-13, ganie2024arsenictoxicitysources pages 1-2)
Arsenite As(III) Trivalent inorganic arsenic; generally more toxic than As(V), strongly interacts with sulfhydryl-containing proteins and key enzymes, and is central to oxidative stress and mitochondrial dysfunction mechanisms. Arsenite; As3+; trivalent arsenic; meta-arsenite Ingestion; inhalation; some dermal absorption of trivalent forms Not found in retrieved sources (ganie2024arsenictoxicitysources pages 2-3, ganie2024arsenictoxicitysources pages 1-2, pereira2025arsenomearsenobolomeand pages 19-20)
Arsenate As(V) Pentavalent inorganic arsenic; enters cells via phosphate transporters and can substitute for phosphate in biochemical reactions, disrupting cellular energetics before reduction/methylation. Arsenate; As5+; pentavalent arsenic Ingestion; inhalation Not found in retrieved sources (ganie2024arsenictoxicitysources pages 2-3, ganie2024arsenictoxicitysources pages 1-2, pereira2025arsenomearsenobolomeand pages 19-20)
Arsine gas AsH3 Extremely toxic gaseous arsenic species; inhalational exposure is a classic occupational hazard and can be rapidly lethal, with reported lethality above ~10 ppm in retrieved evidence. Arsine; arsenic hydride Inhalation Not found in retrieved sources (balalimood2025recentadvancesin pages 12-13)

Table: This table summarizes the main clinical and chemical concepts relevant to arsenic poisoning, including acute and chronic disease forms and major inorganic arsenic species. It is useful as a compact reference for terminology, exposure routes, and evidence-backed definitions from the retrieved literature.

Diagnostics and treatments (summary table)

Category Item What it indicates/when used Key quantitative thresholds or notes Evidence
Diagnostic Urine total arsenic (24-hour) Main biomarker for recent arsenic exposure; used in suspected acute or ongoing exposure 24-hour urinary arsenic >100 µg/L reported as elevated/toxic in retrieved evidence; chelation follow-up target <50 µg/L in 24-hour urine; seafood can confound total urinary arsenic unless speciation is done (pullella2024elucidatingtherelationship pages 37-41, balalimood2025recentadvancesin pages 13-14, pullella2024elucidatingtherelationship pages 41-44) (pullella2024elucidatingtherelationship pages 37-41, balalimood2025recentadvancesin pages 13-14, pullella2024elucidatingtherelationship pages 41-44)
Diagnostic Spot urine arsenic Practical alternative to 24-hour collection for recent exposure assessment Spot urine >50 µg/L reported as elevated in retrieved evidence; should be interpreted with hydration correction (e.g., creatinine adjustment) and ideally with speciation (balalimood2025recentadvancesin pages 12-13, pullella2024elucidatingtherelationship pages 37-41, pullella2024elucidatingtherelationship pages 41-44) (balalimood2025recentadvancesin pages 12-13, pullella2024elucidatingtherelationship pages 37-41, pullella2024elucidatingtherelationship pages 41-44)
Diagnostic Urine arsenic speciation (iAs, MMA, DMA) Best test to distinguish toxic inorganic exposure from seafood-derived organic arsenic; also used to assess methylation phenotype and susceptibility Requires seafood avoidance before testing; elevated urinary MMA or higher MMA/DMA ratio suggests less complete methylation and potentially higher cancer susceptibility; recent exposure window roughly several days because biologic half-life is about 2-4 days (chakif2026heavymetaltoxicity pages 12-13, pullella2024elucidatingtherelationship pages 37-41) (chakif2026heavymetaltoxicity pages 12-13, pullella2024elucidatingtherelationship pages 37-41)
Diagnostic Blood arsenic Reflects very recent exposure and acute poisoning; less useful after rapid clearance Blood arsenic >130 nmol/L reported as elevated/toxic in retrieved evidence; blood half-life about 2-6 h, so sensitivity falls quickly after exposure (pullella2024elucidatingtherelationship pages 37-41, pullella2024elucidatingtherelationship pages 41-44) (pullella2024elucidatingtherelationship pages 37-41, pullella2024elucidatingtherelationship pages 41-44)
Diagnostic Serum arsenic Limited clinical utility because arsenic clears rapidly from blood Not considered reliable for diagnosis once time has elapsed after exposure (balalimood2025recentadvancesin pages 12-13) (balalimood2025recentadvancesin pages 12-13)
Diagnostic Hair arsenic Marker of longer-term past exposure Can become positive about 30 h after exposure; reflects longer-term exposure but is nondiscriminatory for source/species and is not ideal for acute decision-making (balalimood2025recentadvancesin pages 12-13, pullella2024elucidatingtherelationship pages 37-41) (balalimood2025recentadvancesin pages 12-13, pullella2024elucidatingtherelationship pages 37-41)
Diagnostic Nail arsenic (especially toenail) Marker of chronic exposure over prior months Toenail arsenic >0.5 µg/g reported as elevated in retrieved evidence; nails reflect long-term exposure over about 3-6 months (pullella2024elucidatingtherelationship pages 37-41, pullella2024elucidatingtherelationship pages 41-44) (pullella2024elucidatingtherelationship pages 37-41, pullella2024elucidatingtherelationship pages 41-44)
Diagnostic Imaging (abdominal/chest X-ray) Supportive test in acute poisoning to identify radiopaque ingested arsenic material or evaluate complications Used selectively in acute ingestion; not a biomarker of body burden (balalimood2025recentadvancesin pages 12-13) (balalimood2025recentadvancesin pages 12-13)
Diagnostic ECG monitoring Detects cardiotoxicity in acute poisoning Important because acute arsenic can prolong QT/QRS and trigger torsades/arrhythmias (balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13) (balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13)
Treatment Exposure cessation/source removal First-line intervention in all cases, especially chronic/subacute arsenicosis Removal from contaminated water/food/occupational source is the primary treatment for chronic poisoning (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment Succimer / DMSA Oral chelator used for arsenic poisoning, especially when prolonged treatment is needed or less invasive therapy is preferred Named as a key chelator; preferred for prolonged chronic/subacute cases in retrieved evidence; most effective when started minutes to hours after exposure (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment DMPS Water-soluble chelator used in arsenic poisoning Named as a key arsenic chelator; most useful early after exposure; use varies by region/regulatory approval (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment Dimercaprol / BAL Traditional parenteral chelator for severe acute arsenic poisoning Named as a key chelator; most effective when given soon after exposure, typically minutes to hours (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment IV fluids and electrolyte replacement Core supportive therapy for acute poisoning with severe vomiting/diarrhea and shock Critical because deaths often result from hypovolemia, renal failure, or cardiac complications (balalimood2025recentadvancesin pages 12-13, balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 12-13, balalimood2025recentadvancesin pages 13-14)
Treatment GI decontamination / bowel irrigation / NG suction Used in selected acute ingestions, especially if arsenic is still in the GI tract or radiopaque material is seen Activated charcoal adsorbs arsenic poorly; whole-bowel irrigation or continued NG suction may be considered in severe ingestion (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment Antiarrhythmic management and magnesium Supportive management for QT prolongation/torsades and other arrhythmias Magnesium sulfate, amiodarone, or lidocaine reported as options; avoid class IA/IC/III antiarrhythmics in this context per retrieved review (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment Benzodiazepines for seizures Symptom-directed treatment in acute neurotoxicity Used when seizures occur during severe intoxication (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment Hemodialysis Adjunctive/supportive therapy in severe poisoning with kidney failure or oliguria Recommended in acute kidney injury/oliguria; supportive rather than stand-alone antidotal therapy (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment Exchange transfusion Special situation therapy for arsine gas poisoning with massive hemolysis Can help remove arsine-related toxic burden in severe hemolytic presentations (balalimood2025recentadvancesin pages 13-14) (balalimood2025recentadvancesin pages 13-14)
Treatment Antioxidant/nutritional adjuncts Investigational or supportive approaches to mitigate toxicity or improve methylation/detoxification Selenium, zinc, folate, vitamins A/C/E, and phytochemicals have been proposed; clinical evidence remains limited (balalimood2025recentadvancesin pages 13-14, abuawad2023thefolicacid pages 6-7, abuawad2023thefolicacid pages 7-8, abuawad2023thefolicacid pages 1-2) (balalimood2025recentadvancesin pages 13-14, abuawad2023thefolicacid pages 6-7, abuawad2023thefolicacid pages 7-8, abuawad2023thefolicacid pages 1-2)

Table: This table summarizes clinically relevant diagnostic biomarkers and current treatment approaches for arsenic poisoning, including practical interpretation notes and quantitative thresholds reported in the retrieved evidence. It is useful for distinguishing recent versus chronic exposure and for mapping acute management options to the evidence base.

Mechanisms and ontology mapping (summary table)

Mechanism (high level) Molecular details/chain Example genes/proteins Suggested GO biological process terms Suggested CL cell types Suggested UBERON organs/tissues Suggested CHEBI entities/arsenic species Evidence (citation IDs)
Toxicokinetic uptake, biotransformation, and distribution Ingested/inhaled inorganic arsenic is absorbed, distributed systemically, and methylated mainly in liver to MMA and DMA; As(V) enters via phosphate transporters, As(III) via aquaglyceroporins, then binds thiols and undergoes reduction/methylation using GSH, thioredoxin systems, SAM, and AS3MT. Urinary excretion is the main clearance route; skin, hair, nails, bone, and teeth can accumulate arsenic. AS3MT, TXNRD1, thioredoxin, glutathione-related proteins, aquaglyceroporins, phosphate transporters GO:0006730 one-carbon metabolic process; GO:0017144 drug metabolic process; GO:0042493 response to drug; GO:0055085 transmembrane transport hepatocyte; erythrocyte; renal tubular epithelial cell liver (UBERON:0002107); kidney (UBERON:0002113); skin (UBERON:0002097); blood (UBERON:0000178) inorganic arsenic; arsenite(3+) / As(III); arsenate(V) / As(V); monomethylarsonous acid (MMAIII); dimethylarsinic acid (DMA) (ganie2024arsenictoxicitysources pages 2-3, chakif2026heavymetaltoxicity pages 12-13, ganie2024arsenictoxicitysources pages 1-2, pullella2024elucidatingtherelationship pages 37-41)
Thiol binding and enzyme inhibition Trivalent arsenicals bind sulfhydryl/lipoic-acid–dependent enzymes, disrupting central metabolism and redox homeostasis. MMA(III) binds lipoic acid and inhibits pyruvate dehydrogenase; As(V) can substitute for phosphate in metabolic intermediates, impairing ATP-generating reactions. pyruvate dehydrogenase complex, lipoic acid–dependent enzymes, glyceraldehyde-3-phosphate dehydrogenase GO:0006099 tricarboxylic acid cycle; GO:0006096 glycolytic process; GO:0046034 ATP metabolic process; GO:0055114 oxidation-reduction process hepatocyte; cardiomyocyte; neuron liver (UBERON:0002107); heart (UBERON:0000948); nervous system (UBERON:0001016) arsenite(3+); arsenate(V); MMAIII; ADP-arsenate; glucose-6-arsenate (pereira2025arsenomearsenobolomeand pages 19-20, ganie2024arsenictoxicitysources pages 2-3, chakif2026heavymetaltoxicity pages 12-13)
Oxidative and nitrosative stress A central initiating event is ROS/RNS generation with lipid, protein, and DNA oxidation; reported biomarkers include MDA, protein carbonyls, and 8-hydroxydeoxyguanosine. MMA(III) and As(III) also interfere with nitric oxide biology, including NOS inhibition and peroxynitrite-related injury. TXNRD1, Nrf2 pathway components, nitric oxide synthase, heme oxygenase, ferritin, metallothionein GO:0006979 response to oxidative stress; GO:1903409 reactive oxygen species metabolic process; GO:0051409 response to nitrosative stress; GO:0034599 cellular response to oxidative stress fibroblast; endothelial cell; keratinocyte; neuron skin (UBERON:0002097); vasculature (UBERON:0004535); lung (UBERON:0002048); kidney (UBERON:0002113) arsenite(3+); MMAIII; inorganic arsenic (balalimood2025recentadvancesin pages 12-13, pereira2025arsenomearsenobolomeand pages 19-20, pereira2025arsenomearsenobolomeand pages 20-22, pullella2024elucidatingtherelationship pages 37-41, ganie2024arsenictoxicitysources pages 2-3)
NRF2-mediated antioxidant response and detoxification Genetic and experimental studies identify NRF2-linked antioxidant defense, detoxification, and stress-response programs as major regulated responses to arsenic metabolites. Cellular morphology QTL mapped loci including Abcc4 and Txnrd1, supporting genetically controlled variability in response to MMAIII. ABCC4, TXNRD1, NRF2 pathway genes, metallothioneins GO:0034599 cellular response to oxidative stress; GO:0042744 hydrogen peroxide catabolic process; GO:0006805 xenobiotic metabolic process; GO:0046677 response to antibiotic fibroblast; hepatocyte; renal epithelial cell liver (UBERON:0002107); kidney (UBERON:0002113); skin (UBERON:0002097) MMAIII; inorganic arsenic; arsenite(3+) (pullella2024elucidatingtherelationship pages 37-41, ganie2024arsenictoxicitysources pages 2-3)
DNA damage and impaired DNA repair Arsenic increases chromosomal abnormalities, sister chromatid exchange, oxidative DNA damage, and genomic instability. It inhibits DNA mismatch repair and broader DNA repair responses; cmQTL work highlighted DNA repair candidate Xrcc2. Chronic exposure is linked to 8-oxo-dG elevation and repair gene dysregulation. XRCC2, MLH1, MSH2, p53-related pathways GO:0006281 DNA repair; GO:0006974 cellular response to DNA damage stimulus; GO:0036297 interstrand cross-link repair; GO:0006302 double-strand break repair fibroblast; keratinocyte; urothelial cell skin (UBERON:0002097); urinary bladder (UBERON:0001255); lung (UBERON:0002048) inorganic arsenic; arsenite(3+); MMAIII (pereira2025arsenomearsenobolomeand pages 19-20, pereira2025arsenomearsenobolomeand pages 20-22, pullella2024elucidatingtherelationship pages 37-41, ganie2024arsenictoxicitysources pages 2-3)
Epigenetic dysregulation and methyl-donor depletion Arsenic perturbs epigenetic control through SAM depletion, global hypomethylation, locus-specific hypermethylation (e.g., MLH1, MSH2), altered DNMT expression, mitochondrial D-loop hypomethylation, miRNA changes, and m6A-related signaling. Nutritional methyl-donor status (folate, choline, methionine, betaine, B vitamins) modifies toxicity. DNMT1, DNMT3A, DNMT3B, MLH1, MSH2, METTL3, YTHDF2, JAK2, STAT3, AS3MT GO:0006306 DNA methylation; GO:0016573 histone acetylation; GO:0032776 DNA methylation on cytosine; GO:0010608 post-transcriptional regulation of gene expression keratinocyte; hepatocyte; stem/progenitor-like epithelial cell skin (UBERON:0002097); liver (UBERON:0002107); urinary bladder (UBERON:0001255) inorganic arsenic; arsenite(3+); methylated arsenicals (pereira2025arsenomearsenobolomeand pages 20-22, pereira2025arsenomearsenobolomeand pages 19-20, pullella2024elucidatingtherelationship pages 37-41, abuawad2023thefolicacid pages 6-7, abuawad2023thefolicacid pages 7-8, abuawad2023thefolicacid pages 1-2)
Mitochondrial dysfunction and apoptosis Arsenic disrupts mitochondrial respiration and oxidative phosphorylation, lowers ATP production, activates JNK/ERK and GRP78/CHOP stress pathways, and promotes apoptosis/cell death trajectories. These events link upstream redox injury to organ dysfunction and neuro/cardiotoxicity. JNK, ERK, GRP78, CHOP, pyruvate dehydrogenase complex GO:0007005 mitochondrion organization; GO:0008635 activation of apoptotic process; GO:1902600 proton transmembrane transport; GO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress neuron; cardiomyocyte; hepatocyte brain (UBERON:0000955); heart (UBERON:0000948); liver (UBERON:0002107) MMAIII; arsenite(3+); inorganic arsenic (pereira2025arsenomearsenobolomeand pages 19-20, ganie2024arsenictoxicitysources pages 2-3, chakif2026heavymetaltoxicity pages 12-13)
Inflammation, angiogenesis, and malignant transformation Chronic exposure activates inflammatory signaling (including NF-kB-related and CD14-linked profiles), VEGF/angiogenesis, EMT-like programs, and altered signal transduction, helping connect long-term exposure to skin, bladder, lung, kidney, and liver cancers. NFkB, VEGF, EGFR, CD14, p38 MAPK pathway components GO:0006954 inflammatory response; GO:0001525 angiogenesis; GO:0001837 epithelial to mesenchymal transition; GO:0008284 positive regulation of cell population proliferation endothelial cell; macrophage/monocyte; keratinocyte; urothelial cell skin (UBERON:0002097); lung (UBERON:0002048); urinary bladder (UBERON:0001255); kidney (UBERON:0002113) inorganic arsenic; arsenite(3+); methylated arsenicals (pereira2025arsenomearsenobolomeand pages 20-22, pullella2024elucidatingtherelationship pages 37-41, chakif2026heavymetaltoxicity pages 12-13, ganie2024arsenictoxicitysources pages 1-2)
Electrophysiologic cardiotoxicity Arsenic blocks repolarizing potassium currents (IKr, IKs), prolonging QT and predisposing to torsades/arrhythmias; this is a key downstream mechanism in acute severe poisoning. IKr channel, IKs channel GO:0086001 cardiac muscle cell action potential; GO:1903779 regulation of cardiac conduction; GO:0006813 potassium ion transport cardiomyocyte heart (UBERON:0000948) arsenite(3+); inorganic arsenic (pereira2025arsenomearsenobolomeand pages 19-20, balalimood2025recentadvancesin pages 12-13, chakif2026heavymetaltoxicity pages 12-13)
Population genetic susceptibility and gene-by-environment interaction Toxic response varies with arsenic methylation phenotype and genetic background. Human and model-system evidence implicates AS3MT genotype in methylation/toxicity variability, while cell-based QTL mapping identified response loci including Abcc4, Txnrd1, and Xrcc2 under MMAIII exposure. AS3MT, ABCC4, TXNRD1, XRCC2 GO:0006805 xenobiotic metabolic process; GO:0042221 response to chemical; GO:0006974 cellular response to DNA damage stimulus fibroblast; hepatocyte liver (UBERON:0002107); skin (UBERON:0002097); kidney (UBERON:0002113) MMAIII; inorganic arsenic; arsenite(3+) (ganie2024arsenictoxicitysources pages 2-3, pullella2024elucidatingtherelationship pages 37-41, abuawad2023thefolicacid pages 8-9, abuawad2023thefolicacid pages 1-2)

Table: This table summarizes major molecular and cellular mechanisms of arsenic poisoning and links them to suggested ontology terms for knowledge-base curation. It integrates toxicokinetics, oxidative stress, DNA damage, epigenetic dysregulation, mitochondrial injury, carcinogenic signaling, and genetic susceptibility.

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