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3
Mappings
0
Definitions
0
Inheritance
11
Pathophysiology
5
Histopathology
7
Phenotypes
16
Pathograph
9
Genes
4
Treatments
12
Subtypes
0
Differentials
1
Datasets
1
Trials
0
Models
1
Deep Research
🏷

Classifications

Harrison's Chapter
cancer solid tumor
ICD-O Morphology
Embryonal Neoplasm
🔗

Mappings

MONDO
MONDO:0006058 Wilms tumor
skos:exactMatch MONDO
Primary MONDO disease identifier for this Wilms tumor entry.
NCIT
NCIT:C3267 Wilms Tumor
skos:exactMatch NCIT
Primary NCIT disease concept corresponding to Wilms tumor.
ICD-10-CM
ICD10CM:C64.9 Malignant neoplasm of unspecified kidney, except renal pelvis
skos:closeMatch ICD-10-CM
ICD-10-CM C64 category covers malignant neoplasms of kidney including nephroblastoma/Wilms tumor. C64.9 is used when laterality is unspecified. C64.1 (right) and C64.2 (left) specify laterality.
NCIT
NCIT:C3267 Wilms Tumor
skos:exactMatch NCIT
Primary NCIT disease concept corresponding to Wilms tumor.

Subtypes

12
anaplasia status
Favorable Histology Wilms Tumor NCIT:C6951
Approximately 90% of Wilms tumors have favorable histology without anaplasia. These tumors have excellent outcomes with >90% overall survival using current treatment protocols.
NCIT: Nonanaplastic Kidney Wilms Tumor (skos:closeMatch) NCIT:C6951
Anaplastic Wilms Tumor NCIT:C6952
Characterized by extreme nuclear enlargement and pleomorphism with atypical mitoses. May be focal or diffuse. Diffuse anaplasia confers significantly worse prognosis and requires treatment intensification.
NCIT: Anaplastic Kidney Wilms Tumor (skos:closeMatch) NCIT:C6952
histological pattern
Blastemal Predominant Wilms Tumor NCIT:C9147
Blastemal-predominant Wilms tumor is a high-risk histologic pattern, particularly after preoperative chemotherapy, and reflects persistence of residual blastemal cells with relative chemotherapy resistance.
NCIT: Blastema Predominant Kidney Wilms Tumor (skos:closeMatch) NCIT:C9147
Show evidence (1 reference)
PMID:33394739 SUPPORT Human Clinical
"High-risk histology includes blastemal-type tumors, as a predominance of residual blastemal cells is a marker of chemotherapy resistance."
Supports blastemal-predominant Wilms tumor as a distinct histologic pattern with high-risk behavior.
Epithelial Predominant Wilms Tumor NCIT:C9146
Epithelial-predominant Wilms tumor emphasizes tubule- and glomeruloid-like differentiation and generally falls into a more favorable post-chemotherapy histologic group.
NCIT: Epithelial Predominant Kidney Wilms Tumor (skos:closeMatch) NCIT:C9146
Show evidence (1 reference)
PMID:33394739 SUPPORT Human Clinical
"Stromal and epithelia-type nephroblastoma show an excellent outcome after preoperative chemotherapy"
Supports epithelial-predominant Wilms tumor as a recognized histologic pattern with favorable outcomes after preoperative chemotherapy.
Stromal Predominant Wilms Tumor NCIT:C9148
Stromal-predominant Wilms tumor emphasizes mesenchymal differentiation and is generally associated with favorable outcomes after preoperative chemotherapy.
NCIT: Stromal Predominant Kidney Wilms Tumor (skos:closeMatch) NCIT:C9148
Show evidence (1 reference)
PMID:33394739 SUPPORT Human Clinical
"Stromal and epithelia-type nephroblastoma show an excellent outcome after preoperative chemotherapy"
Supports stromal-predominant Wilms tumor as a recognized histologic pattern with favorable post-chemotherapy outcomes.
Mixed Cell Type Wilms Tumor NCIT:C9149
Mixed-cell-type Wilms tumor corresponds to the classic triphasic pattern with blastemal, epithelial, and stromal components in variable proportions.
NCIT: Mixed Cell Type Kidney Wilms Tumor (skos:closeMatch) NCIT:C9149
Show evidence (1 reference)
PMID:27512769 SUPPORT Human Clinical
"Typically, WT comprises three histological components namely blastemal, epithelial and stromal. The proportion and the degree of maturation of these components vary significantly, making the histological appearance of each tumour unique."
Supports mixed-cell-type Wilms tumor as the classic triphasic histologic pattern with variable proportions of blastemal, epithelial, and stromal elements.
laterality
Bilateral Wilms Tumor
Synchronous bilateral tumors occur in approximately 5-10% of cases and are enriched for germline WT1 mutations and post-zygotic epigenetic hypermethylation at 11p15.5. Treatment aims for nephron-sparing approaches to preserve renal function.
Show evidence (1 reference)
PMID:38110397 SUPPORT Human Clinical
"Developing synchronous bilateral Wilms tumor suggests an underlying (epi)genetic predisposition."
Confirms bilateral Wilms tumor is driven by underlying genetic and epigenetic predisposition events, with WT1 germline variants in 14.8% and 11p15.5 epigenetic changes as the predominant events.
Unilateral Wilms Tumor
Most Wilms tumors present as unilateral renal masses involving a single kidney at diagnosis.
predisposition context
Hereditary Predisposition-Associated Wilms Tumor NCIT:C8496
A subset of Wilms tumors arises in the setting of germline or mosaic predisposition, including WT1-associated syndromes and 11p15 epigenetic dysregulation.
NCIT: Hereditary Kidney Wilms Tumor (skos:closeMatch) NCIT:C8496
Show evidence (1 reference)
PMID:37916284 SUPPORT Human Clinical
"In 1990, mutations of the Wilms' tumor-1 gene (WT1), encoding a transcription factor in the embryonic kidney, were found in 10-15% of Wilms' tumors; germline WT1 mutations were associated with hereditary syndromes involving glomerular and reproductive tract dysplasia."
Supports a hereditary predisposition-associated Wilms tumor subgroup linked to germline WT1 alterations and related syndromic presentations.
Sporadic Wilms Tumor
Most Wilms tumors arise sporadically without an identified germline predisposition syndrome, although they still accumulate somatic and epigenetic driver alterations.
age group
Childhood Wilms Tumor MONDO:0024676 NCIT:C27730
The canonical presentation of Wilms tumor is in early childhood, most often before 5 years of age.
NCIT: Childhood Kidney Wilms Tumor (skos:closeMatch) NCIT:C27730
Adult Wilms Tumor NCIT:C6180
Adult Wilms tumor is rare and represents an uncommon age-defined clinical presentation of nephroblastoma.
NCIT: Adult Kidney Wilms Tumor (skos:closeMatch) NCIT:C6180

Pathophysiology

11
WT1 Inactivation
WT1 is a transcription factor essential for normal kidney and gonad development. Biallelic WT1 inactivation occurs in 10-15% of Wilms tumors. Germline WT1 mutations cause WAGR syndrome (Wilms tumor, aniridia, genitourinary anomalies, intellectual disability) and Denys-Drash syndrome. WT1 functions at multiple stages of kidney development, including the transition from resting stem cells to committed nephron progenitor cells.
metanephric mesenchyme stem cell link
cell differentiation link ↓ DECREASED metanephros development link ⚠ ABNORMAL
kidney link
Show evidence (2 references)
PMID:37916284 SUPPORT Human Clinical
"In 1990, mutations of the Wilms' tumor-1 gene (WT1), encoding a transcription factor in the embryonic kidney, were found in 10-15% of Wilms' tumors; germline WT1 mutations were associated with hereditary syndromes involving glomerular and reproductive tract dysplasia."
Confirms WT1 mutation frequency in Wilms tumors and association with hereditary syndromes including WAGR and Denys-Drash.
PMID:37916284 SUPPORT Human Clinical
"WT1 functions at multiple stages of kidney development, including the transition from resting stem cells to committed nephron progenitor, which it primes to respond to WNT9b signals from the ureteric bud."
Details the role of WT1 in kidney development and nephron progenitor cell commitment.
WTX (AMER1) Inactivation
WTX (AMER1) is a component of the beta-catenin destruction complex and a negative regulator of Wnt signaling. WTX inactivation occurs in approximately 15-20% of Wilms tumors and leads to constitutive Wnt pathway activation.
Wnt signaling pathway link ↑ INCREASED
Show evidence (1 reference)
PMID:25018051 SUPPORT Human Clinical
"This has been illustrated by the findings that mutations of Wnt/β-catenin pathway-related WT1, β-catenin, and WTX together account for about one-third of Wilms tumor cases."
Confirms WTX as one of the key Wnt pathway genes mutated in Wilms tumor.
CTNNB1 Activating Mutation
Activating mutations in CTNNB1 (beta-catenin) occur in approximately 15% of Wilms tumors, affecting exon 3 to stabilize beta-catenin and activate canonical Wnt signaling. CTNNB1 mutations are strongly associated with WT1 mutations, promoting proliferation and blocking differentiation.
nephrogenic zone cell link
Wnt signaling pathway link ↑ INCREASED
Show evidence (1 reference)
PMID:25018051 SUPPORT Human Clinical
"This has been illustrated by the findings that mutations of Wnt/β-catenin pathway-related WT1, β-catenin, and WTX together account for about one-third of Wilms tumor cases."
Confirms CTNNB1 as one of the key Wnt pathway genes mutated in Wilms tumor.
Canonical Wnt Signaling Hyperactivation
Aberrant beta-catenin signaling downstream of AMER1 loss or CTNNB1 activation sustains nephrogenic progenitors in a developmental state and cooperates with WT1 loss in Wilms tumor initiation.
nephrogenic zone cell link
Wnt signaling pathway link ↑ INCREASED cell differentiation link ↓ DECREASED
Show evidence (1 reference)
PMID:25018051 SUPPORT Human Clinical
"This has been illustrated by the findings that mutations of Wnt/β-catenin pathway-related WT1, β-catenin, and WTX together account for about one-third of Wilms tumor cases."
Supports canonical Wnt signaling as a major convergent Wilms tumor mechanism downstream of AMER1 and CTNNB1 lesions.
Blocked Nephron Differentiation
Wilms tumor arises from nephrogenic rests - foci of embryonic blastemal cells that persist beyond the normal period of nephrogenesis. These cells retain proliferative capacity but fail to undergo terminal differentiation into mature nephrons. Loss of WT1 in nephrogenic rests may leave these premalignant clones vulnerable to malignant transformation.
metanephric mesenchyme stem cell link
cell population proliferation link ↑ INCREASED nephron development link ⚠ ABNORMAL
Show evidence (2 references)
PMID:37916284 SUPPORT Human Clinical
"Loss of WT1 in nephrogenic rests may leave these premalignant clones with inadequate DNA repair enzymes and may disturb the epigenetic landscape."
Supports the concept that nephrogenic rests with WT1 loss are premalignant precursors of Wilms tumor.
PMID:37385707 SUPPORT Human Clinical
"Wilms tumor (WT), or nephroblastoma, is the most common primary malignant renal tumor of childhood. It is an embryonal tumor that develops from remnants of immature kidney."
Confirms Wilms tumor arises from embryonal remnants of immature kidney tissue.
IGF2 Loss of Imprinting
Loss of imprinting at 11p15, leading to biallelic IGF2 expression, occurs in approximately 70% of Wilms tumors. IGF2 is a major fetal growth factor and its overexpression promotes proliferation of embryonic renal cells. This epigenetic alteration is also the mechanism underlying Beckwith-Wiedemann syndrome, which confers increased Wilms tumor risk.
cell population proliferation link ↑ INCREASED
Show evidence (2 references)
PMID:25018051 SUPPORT Human Clinical
"epigenetic changes, particularly the loss of imprinting of the DNA region encoding the major fetal growth factor IGF2, which results in its biallelic over-expression, are closely associated with the development of many Wilms tumors."
Confirms loss of imprinting at IGF2 locus leading to biallelic overexpression in Wilms tumor development.
PMID:33394739 SUPPORT Human Clinical
"While only 3% of patients without LOH/LOI relapsed, 20% with LOH 11p15 and 25% with LOI 11p15 relapsed"
Demonstrates the prognostic significance of 11p15 LOH/LOI in Wilms tumor, confirming its role in tumor biology.
MicroRNA Processing Defect
DROSHA and DICER1 lesions derepress microRNA target genes controlling differentiation and proliferation, reinforcing a self-renewing mesenchymal state in Wilms tumor.
metanephric mesenchyme stem cell link
DROSHA link DICER1 link
cell differentiation link ↓ DECREASED cell population proliferation link ↑ INCREASED
Show evidence (1 reference)
PMID:25018051 SUPPORT Human Clinical
"Recent investigations also revealed that mutations of Drosha and Dicer, the RNases required for miRNA generation, and Dis3L2, the 3'-5' exonuclease that normally degrades miRNAs and mRNAs, could cause predisposition to Wilms tumors, demonstrating that miRNA can play a pivotal role in Wilms tumor..."
Supports disrupted microRNA biogenesis as a recurrent Wilms tumor mechanism.
Nephron Progenitor Self-Renewal
SIX1 and SIX2 hotspot mutations reinforce the embryonal nephron progenitor transcriptional program in high-risk blastemal Wilms tumors, preserving proliferative potential and chemotherapy resistance.
nephrogenic zone cell link
SIX1 link SIX2 link
cell population proliferation link ↑ INCREASED metanephros development link ⚠ ABNORMAL
Show evidence (1 reference)
PMID:25670083 SUPPORT Human Clinical
"Recurrent mutations included a hotspot mutation (Q177R) in the homeo-domain of SIX1 and SIX2 in tumors with high proliferative potential (18.1% of blastemal cases)"
Supports a SIX1/SIX2-driven progenitor program in proliferative blastemal Wilms tumors.
Persistent Blastemal Progenitor State
Multiple Wilms tumor drivers converge on persistence of embryonal renal blastemal cells with high proliferative capacity and failed terminal differentiation.
metanephric mesenchyme stem cell link nephrogenic zone cell link
cell population proliferation link ↑ INCREASED nephron development link ⚠ ABNORMAL
Show evidence (1 reference)
PMID:25670083 SUPPORT Human Clinical
"Recurrent mutations included a hotspot mutation (Q177R) in the homeo-domain of SIX1 and SIX2 in tumors with high proliferative potential (18.1% of blastemal cases)"
Human tumor genomics support persistence of a highly proliferative blastemal compartment in high-risk Wilms tumor.
Wilms Tumor Outgrowth
Persisting embryonal renal progenitors expand into nephroblastoma, an embryonal kidney malignancy with blastemal, epithelial, and stromal elements.
cell population proliferation link ↑ INCREASED
kidney link
Show evidence (1 reference)
PMID:37385707 SUPPORT Human Clinical
"Wilms tumor (WT), or nephroblastoma, is the most common primary malignant renal tumor of childhood. It is an embryonal tumor that develops from remnants of immature kidney."
Supports the endpoint of embryonal renal outgrowth into overt Wilms tumor.
TP53-Deficient Anaplastic Progression
In anaplastic Wilms tumor, TP53 dysfunction promotes progression toward an aggressive, treatment-resistant state with impaired genome surveillance.
apoptotic process link ↓ DECREASED DNA repair link ↓ DECREASED
Show evidence (1 reference)
PMID:33394739 SUPPORT Human Clinical
"careful analysis including copy number assessment and immunohistochemistry, as well as confirming that anaplasia was present in the portion of tumor undergoing mutation analysis, indicated that nearly all anaplastic WT have TP53 mutations if one looks hard enough"
Supports TP53 dysfunction as the defining progression mechanism in anaplastic Wilms tumor.

Histopathology

5
Triphasic Histology VERY_FREQUENT
Wilms tumor classically shows triphasic histology with blastemal, stromal, and epithelial components. The relative proportions of these components vary and have prognostic significance.
Show evidence (1 reference)
PMID:27512769 SUPPORT Human Clinical
"Typically, WT comprises three histological components namely blastemal, epithelial and stromal. The proportion and the degree of maturation of these components vary significantly, making the histological appearance of each tumour unique. Classical triphasic WT rarely presents diagnostic..."
Directly describes the triphasic histological composition of Wilms tumor with blastemal, epithelial, and stromal components.
Blastemal Predominant Histology
Blastemal-predominant histology is a high-risk Wilms tumor pattern, particularly after preoperative chemotherapy, and reflects persistence of residual blastemal cells with relative chemotherapy resistance.
Show evidence (2 references)
PMID:33394739 SUPPORT Human Clinical
"High-risk histology includes blastemal-type tumors, as a predominance of residual blastemal cells is a marker of chemotherapy resistance."
Directly supports blastemal-predominant Wilms tumor as a distinct high-risk histologic subtype associated with chemotherapy resistance.
PMID:27512769 SUPPORT Human Clinical
"WT with diffuse anaplasia and WT with blastemal predominance (after preoperative chemotherapy) are regarded as high-risk tumours and require more aggressive treatment."
Independent pathology review supporting blastemal predominance as a clinically meaningful high-risk Wilms tumor pattern.
Epithelial Predominant Histology
Epithelial-predominant Wilms tumor emphasizes tubule- and glomeruloid-like epithelial differentiation and generally falls into a more favorable post-chemotherapy histologic group.
Show evidence (2 references)
PMID:33394739 SUPPORT Human Clinical
"Stromal and epithelia-type nephroblastoma show an excellent outcome after preoperative chemotherapy"
Supports epithelial-type nephroblastoma as a recognized histologic group with favorable outcomes after preoperative chemotherapy.
PMID:27512769 SUPPORT Human Clinical
"Typically, WT comprises three histological components namely blastemal, epithelial and stromal. The proportion and the degree of maturation of these components vary significantly, making the histological appearance of each tumour unique."
Supports epithelial-predominant Wilms tumor as part of the variable component balance within Wilms histology.
Stromal Predominant Histology
Stromal-predominant Wilms tumor emphasizes mesenchymal differentiation and, like epithelial-type nephroblastoma, is generally associated with favorable outcomes after preoperative chemotherapy.
Show evidence (2 references)
PMID:33394739 SUPPORT Human Clinical
"Stromal and epithelia-type nephroblastoma show an excellent outcome after preoperative chemotherapy"
Supports stromal-type nephroblastoma as a recognized histologic group with favorable post-chemotherapy outcomes.
PMID:27512769 SUPPORT Human Clinical
"Typically, WT comprises three histological components namely blastemal, epithelial and stromal. The proportion and the degree of maturation of these components vary significantly, making the histological appearance of each tumour unique."
Supports stromal-predominant Wilms tumor as part of the variable component balance within Wilms histology.
Anaplasia
Anaplasia is characterized by extreme nuclear enlargement and pleomorphism with atypical mitoses. It may be focal or diffuse, with diffuse anaplasia conferring significantly worse prognosis. Nearly all anaplastic Wilms tumors harbor TP53 mutations.
Show evidence (1 reference)
PMID:33394739 SUPPORT Human Clinical
"careful analysis including copy number assessment and immunohistochemistry, as well as confirming that anaplasia was present in the portion of tumor undergoing mutation analysis, indicated that nearly all anaplastic WT have TP53 mutations if one looks hard enough"
Confirms near-universal TP53 mutation in anaplastic Wilms tumors.

Pathograph

Use the checkboxes to hide or show graph categories. Hover nodes for evidence and cross-linked metadata.
Pathograph: causal mechanism network for Wilms Tumor Interactive directed graph showing how pathophysiology mechanisms, phenotypes, genetic factors and variants, experimental models, environmental triggers, and treatments relate through causal and linked edges.

Phenotypes

7
Cardiovascular 1
Hypertension FREQUENT Hypertension (HP:0000822)
Show evidence (1 reference)
PMID:33394739 SUPPORT Human Clinical
"However, up to 35% of patients can present with either hematuria, hypertension, fever, or flank pain"
Confirms hypertension as a presenting symptom in Wilms tumor patients.
Digestive 1
Abdominal Mass VERY_FREQUENT Abdominal mass (HP:0031500)
Show evidence (1 reference)
PMID:33394739 SUPPORT Human Clinical
"In most cases, a parent will identify an abdominal mass upon bathing or dressing their child, or a pediatrician will palpate a mass upon examining the child during a routine well-child visit."
Confirms abdominal mass as the most common presenting finding in Wilms tumor.
Eye 1
Aniridia OCCASIONAL Aniridia (HP:0000526)
Show evidence (1 reference)
PMID:33394739 SUPPORT Human Clinical
"More than 15 different syndromes are associated with WT, including WAGR (Wilms tumor, aniridia, genitourinary abnormalities, and a range of developmental delays)"
Confirms aniridia as a feature of WAGR syndrome associated with Wilms tumor.
Genitourinary 1
Hematuria FREQUENT Hematuria (HP:0000790)
Show evidence (1 reference)
PMID:33394739 SUPPORT Human Clinical
"However, up to 35% of patients can present with either hematuria, hypertension, fever, or flank pain"
Confirms hematuria as a presenting symptom in a subset of Wilms tumor patients.
Metabolism 1
Fever OCCASIONAL Fever (HP:0001945)
Show evidence (1 reference)
PMID:33394739 SUPPORT Human Clinical
"However, up to 35% of patients can present with either hematuria, hypertension, fever, or flank pain"
Lists fever among presenting symptoms in Wilms tumor patients.
Constitutional 1
Abdominal Pain OCCASIONAL Abdominal pain (HP:0002027)
Show evidence (1 reference)
PMID:33394739 SUPPORT Human Clinical
"However, up to 35% of patients can present with either hematuria, hypertension, fever, or flank pain"
Confirms flank pain as one of the presenting symptoms of Wilms tumor.
Growth 1
Hemihypertrophy OCCASIONAL Hemihypertrophy (HP:0001528)
Show evidence (1 reference)
PMID:33394739 SUPPORT Human Clinical
"Beckwith-Wiedemann (embryonal tumors, macrosomia, macroglossia, hemihypertrophy, visceromegaly, omphalocele, neonatal hypoglycemia, and ear creases/pits)"
Confirms hemihypertrophy as a feature of Beckwith-Wiedemann syndrome associated with Wilms tumor predisposition.
🧬

Genetic Associations

9
WT1 Mutations (Tumor Suppressor Loss)
Show evidence (1 reference)
PMID:37916284 SUPPORT Human Clinical
"In 1990, mutations of the Wilms' tumor-1 gene (WT1), encoding a transcription factor in the embryonic kidney, were found in 10-15% of Wilms' tumors; germline WT1 mutations were associated with hereditary syndromes involving glomerular and reproductive tract dysplasia."
Confirms WT1 mutation frequency and germline association with hereditary syndromes.
CTNNB1 Mutations (Oncogenic Driver Mutations)
Show evidence (1 reference)
PMID:25018051 SUPPORT Human Clinical
"This has been illustrated by the findings that mutations of Wnt/β-catenin pathway-related WT1, β-catenin, and WTX together account for about one-third of Wilms tumor cases."
Confirms CTNNB1 as one of the key mutated genes in Wilms tumor contributing to Wnt pathway activation.
WTX (AMER1) Mutations (Tumor Suppressor Loss)
Show evidence (1 reference)
PMID:25018051 SUPPORT Human Clinical
"This has been illustrated by the findings that mutations of Wnt/β-catenin pathway-related WT1, β-catenin, and WTX together account for about one-third of Wilms tumor cases."
Confirms WTX (AMER1) as one of the key mutated genes in Wilms tumor.
Loss of 11p15 Imprinting (Epigenetic Alteration)
Show evidence (1 reference)
PMID:25018051 SUPPORT Human Clinical
"epigenetic changes, particularly the loss of imprinting of the DNA region encoding the major fetal growth factor IGF2, which results in its biallelic over-expression, are closely associated with the development of many Wilms tumors."
Confirms loss of imprinting at IGF2/11p15 locus in Wilms tumor development.
Chromosome 1q Gain (Prognostic Marker)
Show evidence (2 references)
PMID:33394739 SUPPORT Human Clinical
"gain of chromosome 1q is one of the most commonly observed cytogenetic abnormalities in WT, seen in as many as 30% of patients"
Confirms 1q gain frequency and its status as a common cytogenetic abnormality in Wilms tumor.
PMID:33394739 SUPPORT Human Clinical
"A retrospective analysis of more than 1000 patients in NWTS-5 demonstrated that 1q gain was associated with inferior EFS and OS across all tumor stages"
Demonstrates prognostic significance of 1q gain across all stages.
Combined LOH 1p/16q (Prognostic Marker)
Show evidence (1 reference)
PMID:33394739 SUPPORT Human Clinical
"Combined LOH 1p and 16q has limited impact as a prognostic marker because only 5% of favorable histology WT carry this molecular change."
Confirms frequency and prognostic relevance of combined 1p/16q LOH.
TP53 Mutations (Anaplasia Driver)
Show evidence (1 reference)
PMID:33394739 SUPPORT Human Clinical
"careful analysis including copy number assessment and immunohistochemistry, as well as confirming that anaplasia was present in the portion of tumor undergoing mutation analysis, indicated that nearly all anaplastic WT have TP53 mutations if one looks hard enough"
Confirms near-universal TP53 mutation in anaplastic Wilms tumors when thoroughly assessed.
DROSHA and DICER1 Mutations (miRNA Processing Defect)
Show evidence (1 reference)
PMID:25018051 SUPPORT Human Clinical
"Recent investigations also revealed that mutations of Drosha and Dicer, the RNases required for miRNA generation, and Dis3L2, the 3'-5' exonuclease that normally degrades miRNAs and mRNAs, could cause predisposition to Wilms tumors, demonstrating that miRNA can play a pivotal role in Wilms tumor..."
Confirms mutations in miRNA processing enzymes as a mechanism of Wilms tumor predisposition.
SIX1/SIX2 Mutations (Nephron Progenitor Program)
Show evidence (1 reference)
PMID:25670083 SUPPORT Human Clinical
"Recurrent mutations included a hotspot mutation (Q177R) in the homeo-domain of SIX1 and SIX2 in tumors with high proliferative potential (18.1% of blastemal cases)"
Landmark genomic study directly demonstrating recurrent SIX1/SIX2 Q177R mutations in 18.1% of blastemal-type Wilms tumors.
💊

Treatments

4
Surgical Resection
Action: nephrectomy Ontology label: Nephrectomy NCIT:C15284
Nephrectomy is the primary treatment for unilateral Wilms tumor. In North America (COG), upfront nephrectomy provides staging information and allows for immediate histologic diagnosis and molecular analysis. In Europe (SIOP), preoperative chemotherapy is given first. Both approaches achieve similar survival rates.
Show evidence (2 references)
PMID:33394739 SUPPORT Human Clinical
"The COG approach of upfront nephrectomy allows for immediate histologic diagnosis, molecular analysis, and accurate local staging assessment."
Confirms the role of nephrectomy as primary surgical approach in COG trials.
PMID:37385707 SUPPORT Human Clinical
"Advances in multimodal therapy including surgery, chemotherapy, and radiation therapy given according to risk stratification have allowed most patients to achieve survival rates in excess of 90%."
Confirms the multimodal approach including surgery achieves >90% survival.
Chemotherapy
Action: chemotherapy Ontology label: Chemotherapy NCIT:C15632
Agent: dactinomycin vincristine doxorubicin
Actinomycin D and vincristine form the backbone of treatment for favorable histology tumors. Doxorubicin and other agents are added for higher-stage or anaplastic tumors. Cyclophosphamide and etoposide are added for the highest-risk patients.
Show evidence (2 references)
PMID:39034876 SUPPORT Human Clinical
"The most common drugs used were vincristine and actinomycin D (78%). Only 12 patients (28%) received anthracyclines."
Confirms vincristine and dactinomycin as backbone chemotherapy with anthracyclines reserved for higher-risk patients.
PMID:33394739 SUPPORT Human Clinical
"Stepwise advances made by the National Wilms Tumor Study Group (NWTSG), which was succeeded by the Children's Oncology Group (COG), and the International Society of Pediatric Oncology (SIOP) Renal Tumor Study Group (RTSG) have not only improved survival to 90%, but have also decreased the burden..."
Confirms that stepwise refinement of chemotherapy has improved survival to 90%.
Radiation Therapy
Action: radiation therapy Ontology label: Radiation Therapy NCIT:C15313
Flank radiation is used for stage III tumors and higher. Whole abdominal radiation is used for tumor rupture or diffuse peritoneal contamination. Whole lung irradiation is used for pulmonary metastases that fail to respond completely to chemotherapy.
Show evidence (1 reference)
PMID:32893998 SUPPORT Human Clinical
"The objectives for the treatment of Wilms tumor in both the Children's Oncology Group (COG) and the International Society of Paediatric Oncology (SIOP) have focused on improving cure rates and minimizing toxicity by limiting the use of radiation and doxorubicin."
Confirms radiation therapy is part of standard treatment while efforts focus on minimizing its use to reduce toxicity.
Selinexor
Action: pharmacotherapy Ontology label: Pharmacotherapy NCIT:C15986
Agent: selinexor
Selinexor is an oral XPO1 inhibitor under clinical evaluation for recurrent or refractory Wilms tumor and other pediatric solid tumors.
Show evidence (1 reference)
clinicaltrials:NCT05985161 SUPPORT Human Clinical
"The purpose of this study is to find out whether selinexor is an effective treatment for people who have a relapsed/refractory Wilms tumor, rhabdoid tumor, MPNST, or another solid tumor that makes a higher than normal amount of XPO1 or has genetic changes that increase the activity of XP01."
Supports selinexor as an investigational Wilms tumor therapy through an active Wilms-specific cohort in a pediatric solid tumor trial.
🔬

Biochemical Markers

2
IGF2 Overexpression
Show evidence (1 reference)
PMID:25018051 SUPPORT Human Clinical
"epigenetic changes, particularly the loss of imprinting of the DNA region encoding the major fetal growth factor IGF2, which results in its biallelic over-expression, are closely associated with the development of many Wilms tumors."
Supports IGF2 overexpression as a canonical Wilms tumor biomarker driven by 11p15 epigenetic dysregulation.
WT1 Protein Expression
📊

Related Datasets

1
TARGET: Kidney, Wilms Tumor (WT) dbgap:phs000471
TARGET high-risk Wilms tumor cohort with matched tumor-normal profiling, including gene expression, copy number, methylation, whole genome sequencing, and subset mRNA-seq, miRNA-seq, and whole exome sequencing.
human MULTI OMICS n=130
Wilms tumor tissue
Conditions: high-risk Wilms tumor anaplastic Wilms tumor relapsed favorable histology Wilms tumor
dbGaP describes 130 fully characterized high-risk Wilms tumor cases with tumor-normal pairs, selected largely from anaplastic tumors or favorable histology tumors that relapsed.
🔬

Clinical Trials

1
NCT05985161 PHASE_II
Multi-center phase II study of selinexor in recurrent or refractory Wilms tumor and other pediatric solid tumors, including a dedicated Wilms tumor cohort.
Show evidence (1 reference)
clinicaltrials:NCT05985161 SUPPORT Human Clinical
"The purpose of this study is to find out whether selinexor is an effective treatment for people who have a relapsed/refractory Wilms tumor, rhabdoid tumor, MPNST, or another solid tumor that makes a higher than normal amount of XPO1 or has genetic changes that increase the activity of XP01."
Supports a Wilms-specific phase II selinexor clinical trial for recurrent or refractory disease.
{ }

Source YAML

click to show
name: Wilms Tumor
creation_date: '2026-01-26T02:55:13Z'
updated_date: '2026-04-13T05:05:42Z'
description: >-
  Wilms tumor (nephroblastoma) is the most common pediatric kidney malignancy,
  typically affecting children under 5 years of age. It exemplifies the concept
  of developmental cancer, arising from persistent embryonic renal blastemal
  cells that fail to undergo normal differentiation. Multiple genes are implicated
  including WT1, WTX (AMER1), and CTNNB1, which together regulate kidney development
  and Wnt signaling. Wilms tumor is one of the great successes of pediatric oncology,
  with cure rates exceeding 90% for favorable histology tumors through a combination
  of surgery, chemotherapy, and risk-adapted radiation. Anaplastic histology confers
  worse prognosis.
categories:
- Pediatric Cancer
- Kidney Cancer
- Solid Tumor
parents:
- kidney neoplasm
has_subtypes:
- name: Favorable Histology
  classification: anaplasia_status
  display_name: Favorable Histology Wilms Tumor
  description: >-
    Approximately 90% of Wilms tumors have favorable histology without anaplasia.
    These tumors have excellent outcomes with >90% overall survival using current
    treatment protocols.
  mappings:
    ncit_mappings:
    - term:
        id: NCIT:C6951
        label: Nonanaplastic Kidney Wilms Tumor
      mapping_predicate: skos:closeMatch
      mapping_source: NCIT
      mapping_justification: NCIT nonanaplastic kidney Wilms tumor closely matches favorable histology Wilms tumor.
- name: Anaplastic
  classification: anaplasia_status
  display_name: Anaplastic Wilms Tumor
  description: >-
    Characterized by extreme nuclear enlargement and pleomorphism with atypical
    mitoses. May be focal or diffuse. Diffuse anaplasia confers significantly
    worse prognosis and requires treatment intensification.
  mappings:
    ncit_mappings:
    - term:
        id: NCIT:C6952
        label: Anaplastic Kidney Wilms Tumor
      mapping_predicate: skos:closeMatch
      mapping_source: NCIT
      mapping_justification: NCIT anaplastic kidney Wilms tumor closely matches the Wilms anaplastic subtype.
- name: Blastemal Predominant
  classification: histological_pattern
  display_name: Blastemal Predominant Wilms Tumor
  description: >-
    Blastemal-predominant Wilms tumor is a high-risk histologic pattern,
    particularly after preoperative chemotherapy, and reflects persistence of
    residual blastemal cells with relative chemotherapy resistance.
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      High-risk histology includes blastemal-type tumors, as a predominance of
      residual blastemal cells is a marker of chemotherapy resistance.
    explanation: >-
      Supports blastemal-predominant Wilms tumor as a distinct histologic
      pattern with high-risk behavior.
  mappings:
    ncit_mappings:
    - term:
        id: NCIT:C9147
        label: Blastema Predominant Kidney Wilms Tumor
      mapping_predicate: skos:closeMatch
      mapping_source: NCIT
      mapping_justification: NCIT blastema-predominant kidney Wilms tumor closely matches this histologic subtype.
- name: Epithelial Predominant
  classification: histological_pattern
  display_name: Epithelial Predominant Wilms Tumor
  description: >-
    Epithelial-predominant Wilms tumor emphasizes tubule- and glomeruloid-like
    differentiation and generally falls into a more favorable post-chemotherapy
    histologic group.
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Stromal and epithelia-type nephroblastoma show an excellent outcome after
      preoperative chemotherapy
    explanation: >-
      Supports epithelial-predominant Wilms tumor as a recognized histologic
      pattern with favorable outcomes after preoperative chemotherapy.
  mappings:
    ncit_mappings:
    - term:
        id: NCIT:C9146
        label: Epithelial Predominant Kidney Wilms Tumor
      mapping_predicate: skos:closeMatch
      mapping_source: NCIT
      mapping_justification: NCIT epithelial-predominant kidney Wilms tumor closely matches this histologic subtype.
- name: Stromal Predominant
  classification: histological_pattern
  display_name: Stromal Predominant Wilms Tumor
  description: >-
    Stromal-predominant Wilms tumor emphasizes mesenchymal differentiation and
    is generally associated with favorable outcomes after preoperative
    chemotherapy.
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Stromal and epithelia-type nephroblastoma show an excellent outcome after
      preoperative chemotherapy
    explanation: >-
      Supports stromal-predominant Wilms tumor as a recognized histologic
      pattern with favorable post-chemotherapy outcomes.
  mappings:
    ncit_mappings:
    - term:
        id: NCIT:C9148
        label: Stromal Predominant Kidney Wilms Tumor
      mapping_predicate: skos:closeMatch
      mapping_source: NCIT
      mapping_justification: NCIT stromal-predominant kidney Wilms tumor closely matches this histologic subtype.
- name: Mixed Cell Type
  classification: histological_pattern
  display_name: Mixed Cell Type Wilms Tumor
  description: >-
    Mixed-cell-type Wilms tumor corresponds to the classic triphasic pattern
    with blastemal, epithelial, and stromal components in variable
    proportions.
  evidence:
  - reference: PMID:27512769
    reference_title: "Wilms’ Tumour – Histology and Differential Diagnosis."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Typically, WT comprises three histological components namely blastemal,
      epithelial and stromal. The proportion and the degree of maturation of these
      components vary significantly, making the histological appearance of each tumour
      unique.
    explanation: >-
      Supports mixed-cell-type Wilms tumor as the classic triphasic histologic
      pattern with variable proportions of blastemal, epithelial, and stromal
      elements.
  mappings:
    ncit_mappings:
    - term:
        id: NCIT:C9149
        label: Mixed Cell Type Kidney Wilms Tumor
      mapping_predicate: skos:closeMatch
      mapping_source: NCIT
      mapping_justification: NCIT mixed-cell-type kidney Wilms tumor closely matches the triphasic mixed-cell histologic subtype.
- name: Bilateral
  classification: laterality
  display_name: Bilateral Wilms Tumor
  description: >-
    Synchronous bilateral tumors occur in approximately 5-10% of cases and are
    enriched for germline WT1 mutations and post-zygotic epigenetic
    hypermethylation at 11p15.5. Treatment aims for nephron-sparing approaches
    to preserve renal function.
  evidence:
  - reference: PMID:38110397
    reference_title: "Genetic and epigenetic features of bilateral Wilms tumor predisposition in patients from the Children's Oncology Group AREN18B5-Q."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Developing synchronous bilateral Wilms tumor suggests an underlying
      (epi)genetic predisposition.
    explanation: >-
      Confirms bilateral Wilms tumor is driven by underlying genetic and
      epigenetic predisposition events, with WT1 germline variants in 14.8%
      and 11p15.5 epigenetic changes as the predominant events.
- name: Unilateral
  classification: laterality
  display_name: Unilateral Wilms Tumor
  description: >-
    Most Wilms tumors present as unilateral renal masses involving a single
    kidney at diagnosis.
- name: Hereditary Predisposition-Associated
  classification: predisposition_context
  display_name: Hereditary Predisposition-Associated Wilms Tumor
  description: >-
    A subset of Wilms tumors arises in the setting of germline or mosaic
    predisposition, including WT1-associated syndromes and 11p15 epigenetic
    dysregulation.
  evidence:
  - reference: PMID:37916284
    reference_title: "Wilms' tumor gene 1: lessons from the interface between kidney development and cancer."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      In 1990, mutations of the Wilms' tumor-1 gene (WT1), encoding a transcription
      factor in the embryonic kidney, were found in 10-15% of Wilms' tumors; germline
      WT1 mutations were associated with hereditary syndromes involving glomerular and
      reproductive tract dysplasia.
    explanation: >-
      Supports a hereditary predisposition-associated Wilms tumor subgroup linked
      to germline WT1 alterations and related syndromic presentations.
  mappings:
    ncit_mappings:
    - term:
        id: NCIT:C8496
        label: Hereditary Kidney Wilms Tumor
      mapping_predicate: skos:closeMatch
      mapping_source: NCIT
      mapping_justification: NCIT hereditary kidney Wilms tumor closely matches hereditary predisposition-associated Wilms tumor.
- name: Sporadic
  classification: predisposition_context
  display_name: Sporadic Wilms Tumor
  description: >-
    Most Wilms tumors arise sporadically without an identified germline
    predisposition syndrome, although they still accumulate somatic and
    epigenetic driver alterations.
- name: Childhood
  classification: age_group
  display_name: Childhood Wilms Tumor
  subtype_term:
    preferred_term: childhood kidney Wilms tumor
    term:
      id: MONDO:0024676
      label: childhood kidney Wilms tumor
  description: >-
    The canonical presentation of Wilms tumor is in early childhood, most often
    before 5 years of age.
  mappings:
    ncit_mappings:
    - term:
        id: NCIT:C27730
        label: Childhood Kidney Wilms Tumor
      mapping_predicate: skos:closeMatch
      mapping_source: NCIT
      mapping_justification: NCIT childhood kidney Wilms tumor closely matches the childhood age-group subtype.
- name: Adult
  classification: age_group
  display_name: Adult Wilms Tumor
  description: >-
    Adult Wilms tumor is rare and represents an uncommon age-defined clinical
    presentation of nephroblastoma.
  mappings:
    ncit_mappings:
    - term:
        id: NCIT:C6180
        label: Adult Kidney Wilms Tumor
      mapping_predicate: skos:closeMatch
      mapping_source: NCIT
      mapping_justification: NCIT adult kidney Wilms tumor closely matches the adult age-group subtype.
disease_term:
  preferred_term: Wilms tumor
  term:
    id: MONDO:0006058
    label: Wilms tumor
pathophysiology:
- name: WT1 Inactivation
  description: >-
    WT1 is a transcription factor essential for normal kidney and gonad development.
    Biallelic WT1 inactivation occurs in 10-15% of Wilms tumors. Germline WT1
    mutations cause WAGR syndrome (Wilms tumor, aniridia, genitourinary anomalies,
    intellectual disability) and Denys-Drash syndrome. WT1 functions at multiple
    stages of kidney development, including the transition from resting stem cells
    to committed nephron progenitor cells.
  cell_types:
  - preferred_term: metanephric mesenchyme stem cell
    term:
      id: CL:0000324
      label: metanephric mesenchyme stem cell
  gene:
    preferred_term: WT1
    modifier: DECREASED
    term:
      id: hgnc:12796
      label: WT1
  gene_products:
  - preferred_term: WT1 protein
    term:
      id: NCIT:C17549
      label: Wilms Tumor Protein
  biological_processes:
  - preferred_term: cell differentiation
    modifier: DECREASED
    term:
      id: GO:0030154
      label: cell differentiation
  - preferred_term: metanephros development
    modifier: ABNORMAL
    term:
      id: GO:0001656
      label: metanephros development
  locations:
  - preferred_term: kidney
    term:
      id: UBERON:0002113
      label: kidney
  evidence:
  - reference: PMID:37916284
    reference_title: "Wilms' tumor gene 1: lessons from the interface between kidney development and cancer."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      In 1990, mutations of the Wilms' tumor-1 gene (WT1), encoding a transcription
      factor in the embryonic kidney, were found in 10-15% of Wilms' tumors; germline
      WT1 mutations were associated with hereditary syndromes involving glomerular and
      reproductive tract dysplasia.
    explanation: >-
      Confirms WT1 mutation frequency in Wilms tumors and association with
      hereditary syndromes including WAGR and Denys-Drash.
  - reference: PMID:37916284
    reference_title: "Wilms' tumor gene 1: lessons from the interface between kidney development and cancer."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      WT1 functions at multiple stages of kidney development, including the transition
      from resting stem cells to committed nephron progenitor, which it primes to
      respond to WNT9b signals from the ureteric bud.
    explanation: >-
      Details the role of WT1 in kidney development and nephron progenitor
      cell commitment.
  downstream:
  - target: Blocked Nephron Differentiation
    description: WT1 loss prevents normal renal blastemal differentiation
- name: WTX (AMER1) Inactivation
  description: >-
    WTX (AMER1) is a component of the beta-catenin destruction complex and a
    negative regulator of Wnt signaling. WTX inactivation occurs in approximately
    15-20% of Wilms tumors and leads to constitutive Wnt pathway activation.
  gene:
    preferred_term: AMER1
    modifier: DECREASED
    term:
      id: hgnc:26837
      label: AMER1
  gene_products:
  - preferred_term: WTX protein
    term:
      id: NCIT:C68721
      label: APC Membrane Recruitment Protein 1
  biological_processes:
  - preferred_term: Wnt signaling pathway
    modifier: INCREASED
    term:
      id: GO:0016055
      label: Wnt signaling pathway
  evidence:
  - reference: PMID:25018051
    reference_title: "The development of Wilms tumor: from WT1 and microRNA to animal models."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      This has been illustrated by the findings that mutations of Wnt/β-catenin
      pathway-related WT1, β-catenin, and WTX together account for about one-third of
      Wilms tumor cases.
    explanation: >-
      Confirms WTX as one of the key Wnt pathway genes mutated in Wilms tumor.
  downstream:
  - target: Canonical Wnt Signaling Hyperactivation
    description: Loss of WTX stabilizes beta-catenin signaling in nephrogenic progenitors
- name: CTNNB1 Activating Mutation
  description: >-
    Activating mutations in CTNNB1 (beta-catenin) occur in approximately 15%
    of Wilms tumors, affecting exon 3 to stabilize beta-catenin and activate
    canonical Wnt signaling. CTNNB1 mutations are strongly associated with
    WT1 mutations, promoting proliferation and blocking differentiation.
  cell_types:
  - preferred_term: nephrogenic zone cell
    term:
      id: CL:0009019
      label: nephrogenic zone cell
  gene:
    preferred_term: CTNNB1
    modifier: INCREASED
    term:
      id: hgnc:2514
      label: CTNNB1
  gene_products:
  - preferred_term: beta-catenin
    term:
      id: NCIT:C17478
      label: Catenin Beta-1
  biological_processes:
  - preferred_term: Wnt signaling pathway
    modifier: INCREASED
    term:
      id: GO:0016055
      label: Wnt signaling pathway
  evidence:
  - reference: PMID:25018051
    reference_title: "The development of Wilms tumor: from WT1 and microRNA to animal models."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      This has been illustrated by the findings that mutations of Wnt/β-catenin
      pathway-related WT1, β-catenin, and WTX together account for about one-third of
      Wilms tumor cases.
    explanation: >-
      Confirms CTNNB1 as one of the key Wnt pathway genes mutated in Wilms tumor.
  downstream:
  - target: Canonical Wnt Signaling Hyperactivation
    description: Exon 3 mutations stabilize beta-catenin and drive constitutive Wnt output
- name: Canonical Wnt Signaling Hyperactivation
  description: >-
    Aberrant beta-catenin signaling downstream of AMER1 loss or CTNNB1 activation
    sustains nephrogenic progenitors in a developmental state and cooperates with
    WT1 loss in Wilms tumor initiation.
  cell_types:
  - preferred_term: nephrogenic zone cell
    term:
      id: CL:0009019
      label: nephrogenic zone cell
  biological_processes:
  - preferred_term: Wnt signaling pathway
    modifier: INCREASED
    term:
      id: GO:0016055
      label: Wnt signaling pathway
  - preferred_term: cell differentiation
    modifier: DECREASED
    term:
      id: GO:0030154
      label: cell differentiation
  evidence:
  - reference: PMID:25018051
    reference_title: "The development of Wilms tumor: from WT1 and microRNA to animal models."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      This has been illustrated by the findings that mutations of Wnt/β-catenin
      pathway-related WT1, β-catenin, and WTX together account for about one-third of
      Wilms tumor cases.
    explanation: >-
      Supports canonical Wnt signaling as a major convergent Wilms tumor mechanism
      downstream of AMER1 and CTNNB1 lesions.
  downstream:
  - target: Blocked Nephron Differentiation
    description: Constitutive beta-catenin signaling prevents normal nephron maturation
  - target: Persistent Blastemal Progenitor State
    description: Wnt output maintains embryonal renal progenitors in a self-renewing compartment
- name: Blocked Nephron Differentiation
  description: >-
    Wilms tumor arises from nephrogenic rests - foci of embryonic blastemal cells
    that persist beyond the normal period of nephrogenesis. These cells retain
    proliferative capacity but fail to undergo terminal differentiation into
    mature nephrons. Loss of WT1 in nephrogenic rests may leave these premalignant
    clones vulnerable to malignant transformation.
  cell_types:
  - preferred_term: metanephric mesenchyme stem cell
    term:
      id: CL:0000324
      label: metanephric mesenchyme stem cell
  biological_processes:
  - preferred_term: cell population proliferation
    modifier: INCREASED
    term:
      id: GO:0008283
      label: cell population proliferation
  - preferred_term: nephron development
    modifier: ABNORMAL
    term:
      id: GO:0072006
      label: nephron development
  evidence:
  - reference: PMID:37916284
    reference_title: "Wilms' tumor gene 1: lessons from the interface between kidney development and cancer."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Loss of WT1 in nephrogenic rests may leave these premalignant clones with
      inadequate DNA repair enzymes and may disturb the epigenetic landscape.
    explanation: >-
      Supports the concept that nephrogenic rests with WT1 loss are premalignant
      precursors of Wilms tumor.
  - reference: PMID:37385707
    reference_title: "Wilms Tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Wilms tumor (WT), or nephroblastoma, is the most common primary malignant renal
      tumor of childhood. It is an embryonal tumor that develops from remnants of
      immature kidney.
    explanation: >-
      Confirms Wilms tumor arises from embryonal remnants of immature kidney tissue.
  downstream:
  - target: Persistent Blastemal Progenitor State
    description: Developmental arrest preserves proliferative embryonal renal cells
- name: IGF2 Loss of Imprinting
  description: >-
    Loss of imprinting at 11p15, leading to biallelic IGF2 expression, occurs
    in approximately 70% of Wilms tumors. IGF2 is a major fetal growth factor
    and its overexpression promotes proliferation of embryonic renal cells. This
    epigenetic alteration is also the mechanism underlying Beckwith-Wiedemann syndrome,
    which confers increased Wilms tumor risk.
  gene:
    preferred_term: IGF2
    modifier: INCREASED
    term:
      id: hgnc:5466
      label: IGF2
  gene_products:
  - preferred_term: IGF2
    term:
      id: NCIT:C16744
      label: Insulin-Like Growth Factor II
  biological_processes:
  - preferred_term: cell population proliferation
    modifier: INCREASED
    term:
      id: GO:0008283
      label: cell population proliferation
  evidence:
  - reference: PMID:25018051
    reference_title: "The development of Wilms tumor: from WT1 and microRNA to animal models."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      epigenetic changes, particularly the loss of imprinting of the DNA region
      encoding the major fetal growth factor IGF2, which results in its biallelic
      over-expression, are closely associated with the development of many Wilms
      tumors.
    explanation: >-
      Confirms loss of imprinting at IGF2 locus leading to biallelic overexpression
      in Wilms tumor development.
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      While only 3% of patients without LOH/LOI relapsed, 20% with LOH 11p15 and
      25% with LOI 11p15 relapsed
    explanation: >-
      Demonstrates the prognostic significance of 11p15 LOH/LOI in Wilms tumor,
      confirming its role in tumor biology.
  downstream:
  - target: Persistent Blastemal Progenitor State
    description: Biallelic IGF2 expression expands fetal renal progenitors
- name: MicroRNA Processing Defect
  description: >-
    DROSHA and DICER1 lesions derepress microRNA target genes controlling
    differentiation and proliferation, reinforcing a self-renewing mesenchymal
    state in Wilms tumor.
  genes:
  - preferred_term: DROSHA
    term:
      id: hgnc:17904
      label: DROSHA
  - preferred_term: DICER1
    term:
      id: hgnc:17098
      label: DICER1
  cell_types:
  - preferred_term: metanephric mesenchyme stem cell
    term:
      id: CL:0000324
      label: metanephric mesenchyme stem cell
  biological_processes:
  - preferred_term: cell differentiation
    modifier: DECREASED
    term:
      id: GO:0030154
      label: cell differentiation
  - preferred_term: cell population proliferation
    modifier: INCREASED
    term:
      id: GO:0008283
      label: cell population proliferation
  evidence:
  - reference: PMID:25018051
    reference_title: "The development of Wilms tumor: from WT1 and microRNA to animal models."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Recent investigations also revealed that mutations of Drosha and Dicer, the
      RNases required for miRNA generation, and Dis3L2, the 3'-5' exonuclease that
      normally degrades miRNAs and mRNAs, could cause predisposition to Wilms tumors,
      demonstrating that miRNA can play a pivotal role in Wilms tumor development.
    explanation: >-
      Supports disrupted microRNA biogenesis as a recurrent Wilms tumor mechanism.
  downstream:
  - target: Persistent Blastemal Progenitor State
    description: miRNA dysregulation reinforces the undifferentiated proliferative compartment
- name: Nephron Progenitor Self-Renewal
  description: >-
    SIX1 and SIX2 hotspot mutations reinforce the embryonal nephron progenitor
    transcriptional program in high-risk blastemal Wilms tumors, preserving
    proliferative potential and chemotherapy resistance.
  genes:
  - preferred_term: SIX1
    term:
      id: hgnc:10887
      label: SIX1
  - preferred_term: SIX2
    term:
      id: hgnc:10888
      label: SIX2
  cell_types:
  - preferred_term: nephrogenic zone cell
    term:
      id: CL:0009019
      label: nephrogenic zone cell
  biological_processes:
  - preferred_term: cell population proliferation
    modifier: INCREASED
    term:
      id: GO:0008283
      label: cell population proliferation
  - preferred_term: metanephros development
    modifier: ABNORMAL
    term:
      id: GO:0001656
      label: metanephros development
  evidence:
  - reference: PMID:25670083
    reference_title: "Mutations in the SIX1/2 pathway and the DROSHA/DGCR8 miRNA microprocessor complex underlie high-risk blastemal type Wilms tumors."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Recurrent mutations included a hotspot mutation (Q177R) in the homeo-domain
      of SIX1 and SIX2 in tumors with high proliferative potential (18.1% of
      blastemal cases)
    explanation: >-
      Supports a SIX1/SIX2-driven progenitor program in proliferative blastemal
      Wilms tumors.
  downstream:
  - target: Persistent Blastemal Progenitor State
    description: SIX1/SIX2-mutant progenitors retain a proliferative embryonal identity
- name: Persistent Blastemal Progenitor State
  description: >-
    Multiple Wilms tumor drivers converge on persistence of embryonal renal
    blastemal cells with high proliferative capacity and failed terminal
    differentiation.
  cell_types:
  - preferred_term: metanephric mesenchyme stem cell
    term:
      id: CL:0000324
      label: metanephric mesenchyme stem cell
  - preferred_term: nephrogenic zone cell
    term:
      id: CL:0009019
      label: nephrogenic zone cell
  biological_processes:
  - preferred_term: cell population proliferation
    modifier: INCREASED
    term:
      id: GO:0008283
      label: cell population proliferation
  - preferred_term: nephron development
    modifier: ABNORMAL
    term:
      id: GO:0072006
      label: nephron development
  evidence:
  - reference: PMID:25670083
    reference_title: "Mutations in the SIX1/2 pathway and the DROSHA/DGCR8 miRNA microprocessor complex underlie high-risk blastemal type Wilms tumors."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Recurrent mutations included a hotspot mutation (Q177R) in the homeo-domain
      of SIX1 and SIX2 in tumors with high proliferative potential (18.1% of
      blastemal cases)
    explanation: >-
      Human tumor genomics support persistence of a highly proliferative blastemal
      compartment in high-risk Wilms tumor.
  downstream:
  - target: Wilms Tumor Outgrowth
    description: Persisting blastemal clones expand into overt nephroblastoma
- name: Wilms Tumor Outgrowth
  description: >-
    Persisting embryonal renal progenitors expand into nephroblastoma, an
    embryonal kidney malignancy with blastemal, epithelial, and stromal elements.
  locations:
  - preferred_term: kidney
    term:
      id: UBERON:0002113
      label: kidney
  biological_processes:
  - preferred_term: cell population proliferation
    modifier: INCREASED
    term:
      id: GO:0008283
      label: cell population proliferation
  evidence:
  - reference: PMID:37385707
    reference_title: "Wilms Tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Wilms tumor (WT), or nephroblastoma, is the most common primary malignant renal
      tumor of childhood. It is an embryonal tumor that develops from remnants of
      immature kidney.
    explanation: >-
      Supports the endpoint of embryonal renal outgrowth into overt Wilms tumor.
  downstream:
  - target: TP53-Deficient Anaplastic Progression
    description: Acquisition of TP53 dysfunction drives aggressive anaplastic evolution in a subset of tumors
- name: TP53-Deficient Anaplastic Progression
  description: >-
    In anaplastic Wilms tumor, TP53 dysfunction promotes progression toward an
    aggressive, treatment-resistant state with impaired genome surveillance.
  gene:
    preferred_term: TP53
    modifier: DECREASED
    term:
      id: hgnc:11998
      label: TP53
  biological_processes:
  - preferred_term: apoptotic process
    modifier: DECREASED
    term:
      id: GO:0006915
      label: apoptotic process
  - preferred_term: DNA repair
    modifier: DECREASED
    term:
      id: GO:0006281
      label: DNA repair
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      careful analysis including copy number assessment and immunohistochemistry,
      as well as confirming that anaplasia was present in the portion of tumor
      undergoing mutation analysis, indicated that nearly all anaplastic WT have
      TP53 mutations if one looks hard enough
    explanation: >-
      Supports TP53 dysfunction as the defining progression mechanism in anaplastic
      Wilms tumor.
phenotypes:
- category: Abdominal
  name: Abdominal Mass
  frequency: VERY_FREQUENT
  diagnostic: true
  description: >-
    A palpable abdominal mass is the most common presenting finding, often
    noticed incidentally by parents during bathing. The mass is typically
    smooth, firm, and does not cross the midline.
  phenotype_term:
    preferred_term: Abdominal mass
    term:
      id: HP:0031500
      label: Abdominal mass
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      In most cases, a parent will identify an abdominal mass upon bathing or dressing
      their child, or a pediatrician will palpate a mass upon examining the child
      during a routine well-child visit.
    explanation: >-
      Confirms abdominal mass as the most common presenting finding in Wilms tumor.
- category: Genitourinary
  name: Hematuria
  frequency: FREQUENT
  description: >-
    Gross or microscopic hematuria occurs in patients presenting with Wilms tumor
    and may be the presenting symptom.
  phenotype_term:
    preferred_term: Hematuria
    term:
      id: HP:0000790
      label: Hematuria
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      However, up to 35% of patients can present with either hematuria, hypertension,
      fever, or flank pain
    explanation: >-
      Confirms hematuria as a presenting symptom in a subset of Wilms tumor patients.
- category: Cardiovascular
  name: Hypertension
  frequency: FREQUENT
  description: >-
    Hypertension occurs in patients with Wilms tumor due to renin secretion
    by the tumor or renal artery compression.
  phenotype_term:
    preferred_term: Hypertension
    term:
      id: HP:0000822
      label: Hypertension
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      However, up to 35% of patients can present with either hematuria, hypertension,
      fever, or flank pain
    explanation: >-
      Confirms hypertension as a presenting symptom in Wilms tumor patients.
- category: Constitutional
  name: Fever
  frequency: OCCASIONAL
  description: >-
    Low-grade fever may occur as a constitutional symptom.
  phenotype_term:
    preferred_term: Fever
    term:
      id: HP:0001945
      label: Fever
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      However, up to 35% of patients can present with either hematuria, hypertension,
      fever, or flank pain
    explanation: >-
      Lists fever among presenting symptoms in Wilms tumor patients.
- category: Ophthalmologic
  name: Aniridia
  frequency: OCCASIONAL
  description: >-
    Aniridia (absence of the iris) occurs in WAGR syndrome, caused by contiguous
    deletion of WT1 and PAX6 on chromosome 11p13. Children with sporadic aniridia
    require screening for Wilms tumor.
  phenotype_term:
    preferred_term: Aniridia
    term:
      id: HP:0000526
      label: Aniridia
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      More than 15 different syndromes are associated with WT, including WAGR
      (Wilms tumor, aniridia, genitourinary abnormalities, and a range of
      developmental delays)
    explanation: >-
      Confirms aniridia as a feature of WAGR syndrome associated with Wilms tumor.
- category: Growth
  name: Hemihypertrophy
  frequency: OCCASIONAL
  description: >-
    Hemihypertrophy (asymmetric overgrowth) is associated with Beckwith-Wiedemann
    syndrome and increased Wilms tumor risk. Children with hemihypertrophy
    require tumor surveillance.
  phenotype_term:
    preferred_term: Hemihypertrophy
    term:
      id: HP:0001528
      label: Hemihypertrophy
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Beckwith-Wiedemann (embryonal tumors, macrosomia, macroglossia, hemihypertrophy,
      visceromegaly, omphalocele, neonatal hypoglycemia, and ear creases/pits)
    explanation: >-
      Confirms hemihypertrophy as a feature of Beckwith-Wiedemann syndrome
      associated with Wilms tumor predisposition.
- category: Abdominal
  name: Abdominal Pain
  frequency: OCCASIONAL
  description: >-
    Flank pain may be a presenting symptom, and rarely, patients present with
    acute abdomen in the setting of tumor rupture.
  phenotype_term:
    preferred_term: Abdominal pain
    term:
      id: HP:0002027
      label: Abdominal pain
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      However, up to 35% of patients can present with either hematuria, hypertension,
      fever, or flank pain
    explanation: >-
      Confirms flank pain as one of the presenting symptoms of Wilms tumor.
histopathology:
- name: Triphasic Histology
  subtype: Mixed Cell Type
  finding_term:
    preferred_term: triphasic pattern
    term:
      id: NCIT:C35928
      label: Triphasic Pattern
  frequency: VERY_FREQUENT
  description: >-
    Wilms tumor classically shows triphasic histology with blastemal, stromal,
    and epithelial components. The relative proportions of these components
    vary and have prognostic significance.
  evidence:
  - reference: PMID:27512769
    reference_title: "Wilms’ Tumour – Histology and Differential Diagnosis."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Typically, WT comprises three histological components namely blastemal,
      epithelial and stromal. The proportion and the degree of maturation of these
      components vary significantly, making the histological appearance of each tumour
      unique. Classical triphasic WT rarely presents diagnostic difficulty for
      pathologists
    explanation: >-
      Directly describes the triphasic histological composition of Wilms tumor
      with blastemal, epithelial, and stromal components.
- name: Blastemal Predominant Histology
  subtype: Blastemal Predominant
  finding_term:
    preferred_term: Blastema Predominant Kidney Wilms Tumor
    term:
      id: NCIT:C9147
      label: Blastema Predominant Kidney Wilms Tumor
  description: >-
    Blastemal-predominant histology is a high-risk Wilms tumor pattern,
    particularly after preoperative chemotherapy, and reflects persistence of
    residual blastemal cells with relative chemotherapy resistance.
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      High-risk histology includes blastemal-type tumors, as a predominance of
      residual blastemal cells is a marker of chemotherapy resistance.
    explanation: >-
      Directly supports blastemal-predominant Wilms tumor as a distinct
      high-risk histologic subtype associated with chemotherapy resistance.
  - reference: PMID:27512769
    reference_title: "Wilms’ Tumour – Histology and Differential Diagnosis."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      WT with diffuse anaplasia and WT with blastemal predominance (after
      preoperative chemotherapy) are regarded as high-risk tumours and require more
      aggressive treatment.
    explanation: >-
      Independent pathology review supporting blastemal predominance as a
      clinically meaningful high-risk Wilms tumor pattern.
- name: Epithelial Predominant Histology
  subtype: Epithelial Predominant
  finding_term:
    preferred_term: Epithelial Predominant Kidney Wilms Tumor
    term:
      id: NCIT:C9146
      label: Epithelial Predominant Kidney Wilms Tumor
  description: >-
    Epithelial-predominant Wilms tumor emphasizes tubule- and glomeruloid-like
    epithelial differentiation and generally falls into a more favorable
    post-chemotherapy histologic group.
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Stromal and epithelia-type nephroblastoma show an excellent outcome after
      preoperative chemotherapy
    explanation: >-
      Supports epithelial-type nephroblastoma as a recognized histologic group
      with favorable outcomes after preoperative chemotherapy.
  - reference: PMID:27512769
    reference_title: "Wilms’ Tumour – Histology and Differential Diagnosis."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Typically, WT comprises three histological components namely blastemal,
      epithelial and stromal. The proportion and the degree of maturation of these
      components vary significantly, making the histological appearance of each tumour
      unique.
    explanation: >-
      Supports epithelial-predominant Wilms tumor as part of the variable
      component balance within Wilms histology.
- name: Stromal Predominant Histology
  subtype: Stromal Predominant
  finding_term:
    preferred_term: Stromal Predominant Kidney Wilms Tumor
    term:
      id: NCIT:C9148
      label: Stromal Predominant Kidney Wilms Tumor
  description: >-
    Stromal-predominant Wilms tumor emphasizes mesenchymal differentiation and,
    like epithelial-type nephroblastoma, is generally associated with favorable
    outcomes after preoperative chemotherapy.
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Stromal and epithelia-type nephroblastoma show an excellent outcome after
      preoperative chemotherapy
    explanation: >-
      Supports stromal-type nephroblastoma as a recognized histologic group with
      favorable post-chemotherapy outcomes.
  - reference: PMID:27512769
    reference_title: "Wilms’ Tumour – Histology and Differential Diagnosis."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Typically, WT comprises three histological components namely blastemal,
      epithelial and stromal. The proportion and the degree of maturation of these
      components vary significantly, making the histological appearance of each tumour
      unique.
    explanation: >-
      Supports stromal-predominant Wilms tumor as part of the variable
      component balance within Wilms histology.
- name: Anaplasia
  subtype: Anaplastic
  finding_term:
    preferred_term: Anaplastic Wilms Tumor
    term:
      id: NCIT:C6952
      label: Anaplastic Kidney Wilms Tumor
  description: >-
    Anaplasia is characterized by extreme nuclear enlargement and pleomorphism
    with atypical mitoses. It may be focal or diffuse, with diffuse anaplasia
    conferring significantly worse prognosis. Nearly all anaplastic Wilms tumors
    harbor TP53 mutations.
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      careful analysis including copy number assessment and immunohistochemistry,
      as well as confirming that anaplasia was present in the portion of tumor
      undergoing mutation analysis, indicated that nearly all anaplastic WT have
      TP53 mutations if one looks hard enough
    explanation: >-
      Confirms near-universal TP53 mutation in anaplastic Wilms tumors.
biochemical:
- name: IGF2 Overexpression
  biomarker_term:
    preferred_term: IGF2
    term:
      id: NCIT:C16744
      label: Insulin-Like Growth Factor II
  notes: >-
    IGF2 overexpression is a hallmark Wilms tumor biomarker linked to 11p15
    loss of imprinting and biallelic fetal growth-factor expression, particularly
    in blastemal and predisposition-associated tumors.
  evidence:
  - reference: PMID:25018051
    reference_title: "The development of Wilms tumor: from WT1 and microRNA to animal models."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      epigenetic changes, particularly the loss of imprinting of the DNA region
      encoding the major fetal growth factor IGF2, which results in its biallelic
      over-expression, are closely associated with the development of many Wilms
      tumors.
    explanation: >-
      Supports IGF2 overexpression as a canonical Wilms tumor biomarker driven by
      11p15 epigenetic dysregulation.
- name: WT1 Protein Expression
  biomarker_term:
    preferred_term: WT1 protein
    term:
      id: NCIT:C17549
      label: Wilms Tumor Protein
  notes: >-
    WT1 protein expression is widely used in the diagnostic workup of pediatric
    renal tumors and nephrogenic lesions, complementing genomic assessment of
    WT1 loss or predisposition.
genetic:
- name: WT1 Mutations
  association: Tumor Suppressor Loss
  gene_term:
    preferred_term: WT1
    term:
      id: hgnc:12796
      label: WT1
  notes: >-
    WT1 mutations occur in 10-15% of sporadic Wilms tumors. Germline WT1 mutations
    cause predisposition syndromes (WAGR, Denys-Drash, Frasier). WT1 is a transcription
    factor essential for kidney development.
  evidence:
  - reference: PMID:37916284
    reference_title: "Wilms' tumor gene 1: lessons from the interface between kidney development and cancer."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      In 1990, mutations of the Wilms' tumor-1 gene (WT1), encoding a transcription
      factor in the embryonic kidney, were found in 10-15% of Wilms' tumors; germline
      WT1 mutations were associated with hereditary syndromes involving glomerular and
      reproductive tract dysplasia.
    explanation: >-
      Confirms WT1 mutation frequency and germline association with hereditary
      syndromes.
- name: CTNNB1 Mutations
  association: Oncogenic Driver Mutations
  gene_term:
    preferred_term: CTNNB1
    term:
      id: hgnc:2514
      label: CTNNB1
  notes: >-
    Activating mutations in CTNNB1 (beta-catenin) occur in approximately 15%
    of Wilms tumors, leading to constitutive Wnt pathway activation.
  evidence:
  - reference: PMID:25018051
    reference_title: "The development of Wilms tumor: from WT1 and microRNA to animal models."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      This has been illustrated by the findings that mutations of Wnt/β-catenin
      pathway-related WT1, β-catenin, and WTX together account for about one-third of
      Wilms tumor cases.
    explanation: >-
      Confirms CTNNB1 as one of the key mutated genes in Wilms tumor
      contributing to Wnt pathway activation.
- name: WTX (AMER1) Mutations
  association: Tumor Suppressor Loss
  gene_term:
    preferred_term: AMER1
    term:
      id: hgnc:26837
      label: AMER1
  notes: >-
    WTX inactivation occurs in 15-20% of Wilms tumors. WTX negatively regulates
    Wnt signaling, and its loss leads to pathway activation.
  evidence:
  - reference: PMID:25018051
    reference_title: "The development of Wilms tumor: from WT1 and microRNA to animal models."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      This has been illustrated by the findings that mutations of Wnt/β-catenin
      pathway-related WT1, β-catenin, and WTX together account for about one-third of
      Wilms tumor cases.
    explanation: >-
      Confirms WTX (AMER1) as one of the key mutated genes in Wilms tumor.
- name: Loss of 11p15 Imprinting
  association: Epigenetic Alteration
  gene_term:
    preferred_term: IGF2
    term:
      id: hgnc:5466
      label: IGF2
  notes: >-
    Loss of imprinting at 11p15, leading to biallelic IGF2 expression, occurs
    in approximately 70% of Wilms tumors. This is also the mechanism in
    Beckwith-Wiedemann syndrome.
  evidence:
  - reference: PMID:25018051
    reference_title: "The development of Wilms tumor: from WT1 and microRNA to animal models."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      epigenetic changes, particularly the loss of imprinting of the DNA region
      encoding the major fetal growth factor IGF2, which results in its biallelic
      over-expression, are closely associated with the development of many Wilms
      tumors.
    explanation: >-
      Confirms loss of imprinting at IGF2/11p15 locus in Wilms tumor development.
- name: Chromosome 1q Gain
  association: Prognostic Marker
  notes: >-
    Gain of chromosome 1q is one of the most commonly observed cytogenetic
    abnormalities in Wilms tumor, seen in approximately 30% of patients.
    It is associated with inferior event-free survival and overall survival
    across all tumor stages.
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      gain of chromosome 1q is one of the most commonly observed cytogenetic
      abnormalities in WT, seen in as many as 30% of patients
    explanation: >-
      Confirms 1q gain frequency and its status as a common cytogenetic
      abnormality in Wilms tumor.
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      A retrospective analysis of more than 1000 patients in NWTS-5 demonstrated
      that 1q gain was associated with inferior EFS and OS across all tumor stages
    explanation: >-
      Demonstrates prognostic significance of 1q gain across all stages.
- name: Combined LOH 1p/16q
  association: Prognostic Marker
  notes: >-
    Loss of heterozygosity at chromosomes 1p and 16q occurs in approximately
    5% of favorable histology Wilms tumors and is associated with increased
    risk of relapse. Escalated therapy can overcome this adverse prognostic factor.
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Combined LOH 1p and 16q has limited impact as a prognostic marker because
      only 5% of favorable histology WT carry this molecular change.
    explanation: >-
      Confirms frequency and prognostic relevance of combined 1p/16q LOH.
- name: TP53 Mutations
  association: Anaplasia Driver
  subtype: Anaplastic
  gene_term:
    preferred_term: TP53
    term:
      id: hgnc:11998
      label: TP53
  notes: >-
    TP53 mutations are found in approximately 50-60% of diffuse anaplastic
    Wilms tumors. TP53 dysfunction drives anaplastic transformation and is
    associated with poor prognosis and therapy resistance.
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      careful analysis including copy number assessment and immunohistochemistry,
      as well as confirming that anaplasia was present in the portion of tumor
      undergoing mutation analysis, indicated that nearly all anaplastic WT have
      TP53 mutations if one looks hard enough
    explanation: >-
      Confirms near-universal TP53 mutation in anaplastic Wilms tumors when
      thoroughly assessed.
- name: DROSHA and DICER1 Mutations
  association: miRNA Processing Defect
  notes: >-
    Mutations in DROSHA and DICER1, the RNases required for miRNA generation,
    can cause predisposition to Wilms tumors. DROSHA mutations are correlated
    with a self-renewing mesenchymal state and aberrant redox metabolism.
    Wilms tumor cells lacking microRNAs are sensitized to ferroptotic cell death
    through inhibition of GPX4.
  evidence:
  - reference: PMID:25018051
    reference_title: "The development of Wilms tumor: from WT1 and microRNA to animal models."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Recent investigations also revealed that mutations of Drosha and Dicer, the
      RNases required for miRNA generation, and Dis3L2, the 3'-5' exonuclease that
      normally degrades miRNAs and mRNAs, could cause predisposition to Wilms tumors,
      demonstrating that miRNA can play a pivotal role in Wilms tumor development.
    explanation: >-
      Confirms mutations in miRNA processing enzymes as a mechanism of Wilms
      tumor predisposition.
- name: SIX1/SIX2 Mutations
  association: Nephron Progenitor Program
  notes: >-
    Recurrent SIX1/SIX2 Q177R homeodomain mutations occur in 18.1% of
    blastemal-type Wilms tumors. These mutations are associated with high
    proliferative potential and chemotherapy-resistant blastemal tumors.
  evidence:
  - reference: PMID:25670083
    reference_title: "Mutations in the SIX1/2 pathway and the DROSHA/DGCR8 miRNA microprocessor complex underlie high-risk blastemal type Wilms tumors."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Recurrent mutations included a hotspot mutation (Q177R) in the homeo-domain
      of SIX1 and SIX2 in tumors with high proliferative potential (18.1% of
      blastemal cases)
    explanation: >-
      Landmark genomic study directly demonstrating recurrent SIX1/SIX2 Q177R
      mutations in 18.1% of blastemal-type Wilms tumors.
treatments:
- name: Surgical Resection
  description: >-
    Nephrectomy is the primary treatment for unilateral Wilms tumor. In North
    America (COG), upfront nephrectomy provides staging information and allows
    for immediate histologic diagnosis and molecular analysis. In Europe (SIOP),
    preoperative chemotherapy is given first. Both approaches achieve similar
    survival rates.
  treatment_term:
    preferred_term: nephrectomy
    term:
      id: NCIT:C15284
      label: Nephrectomy
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The COG approach of upfront nephrectomy allows for immediate histologic
      diagnosis, molecular analysis, and accurate local staging assessment.
    explanation: >-
      Confirms the role of nephrectomy as primary surgical approach in COG trials.
  - reference: PMID:37385707
    reference_title: "Wilms Tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Advances in multimodal therapy including surgery, chemotherapy, and radiation
      therapy given according to risk stratification have allowed most patients to
      achieve survival rates in excess of 90%.
    explanation: >-
      Confirms the multimodal approach including surgery achieves >90% survival.
- name: Chemotherapy
  description: >-
    Actinomycin D and vincristine form the backbone of treatment for favorable
    histology tumors. Doxorubicin and other agents are added for higher-stage
    or anaplastic tumors. Cyclophosphamide and etoposide are added for the
    highest-risk patients.
  treatment_term:
    preferred_term: chemotherapy
    term:
      id: NCIT:C15632
      label: Chemotherapy
    therapeutic_agent:
    - preferred_term: dactinomycin
      term:
        id: NCIT:C412
        label: Dactinomycin
    - preferred_term: vincristine
      term:
        id: NCIT:C933
        label: Vincristine
    - preferred_term: doxorubicin
      term:
        id: NCIT:C456
        label: Doxorubicin
  evidence:
  - reference: PMID:39034876
    reference_title: "Late effects of Wilms' tumor treatment."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The most common drugs used were vincristine and actinomycin D (78%). Only
      12 patients (28%) received anthracyclines.
    explanation: >-
      Confirms vincristine and dactinomycin as backbone chemotherapy with
      anthracyclines reserved for higher-risk patients.
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Stepwise advances made by the National Wilms Tumor Study Group (NWTSG),
      which was succeeded by the Children's Oncology Group (COG), and the
      International Society of Pediatric Oncology (SIOP) Renal Tumor Study Group
      (RTSG) have not only improved survival to 90%, but have also decreased the
      burden of therapy
    explanation: >-
      Confirms that stepwise refinement of chemotherapy has improved survival
      to 90%.
- name: Radiation Therapy
  description: >-
    Flank radiation is used for stage III tumors and higher. Whole abdominal
    radiation is used for tumor rupture or diffuse peritoneal contamination.
    Whole lung irradiation is used for pulmonary metastases that fail to
    respond completely to chemotherapy.
  treatment_term:
    preferred_term: radiation therapy
    term:
      id: NCIT:C15313
      label: Radiation Therapy
  evidence:
  - reference: PMID:32893998
    reference_title: "Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The objectives for the treatment of Wilms tumor in both the Children's Oncology
      Group (COG) and the International Society of Paediatric Oncology (SIOP) have
      focused on improving cure rates and minimizing toxicity by limiting the use of
      radiation and doxorubicin.
    explanation: >-
      Confirms radiation therapy is part of standard treatment while efforts
      focus on minimizing its use to reduce toxicity.
- name: Selinexor
  description: >-
    Selinexor is an oral XPO1 inhibitor under clinical evaluation for recurrent
    or refractory Wilms tumor and other pediatric solid tumors.
  treatment_term:
    preferred_term: pharmacotherapy
    term:
      id: NCIT:C15986
      label: Pharmacotherapy
    therapeutic_agent:
    - preferred_term: selinexor
      term:
        id: NCIT:C102546
        label: Selinexor
  evidence:
  - reference: clinicaltrials:NCT05985161
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The purpose of this study is to find out whether selinexor is an effective
      treatment for people who have a relapsed/refractory Wilms tumor, rhabdoid
      tumor, MPNST, or another solid tumor that makes a higher than normal amount
      of XPO1 or has genetic changes that increase the activity of XP01.
    explanation: >-
      Supports selinexor as an investigational Wilms tumor therapy through an
      active Wilms-specific cohort in a pediatric solid tumor trial.
prevalence:
- population: Worldwide
  notes: >-
    Estimated incidence of about 9 per million children under 15 years old
    in the United States and Canada, affecting 1 in 10,000 children. Similar
    rates in Europe, Australia, and New Zealand with lower rates in Asia
    and higher rates in parts of Africa.
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      In the United States and Canada, the estimated incidence is about 9 per
      million children under 15 years old, affecting 1 in 10,000 children
    explanation: >-
      Provides incidence data for Wilms tumor in North America.
progression:
- phase: Diagnosis
  age_range: Under 5 years
  notes: >-
    Most cases are diagnosed in children under 5 years of age. Increasing patient
    age is associated with increased risk of recurrence and worse outcome.
  evidence:
  - reference: PMID:33394739
    reference_title: "New approaches to risk stratification for Wilms tumor."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      Because WT is a malignant embryonal tumor, most cases are diagnosed in
      children under the age of 5 years.
    explanation: >-
      Confirms typical age at diagnosis for Wilms tumor.
classifications:
  icdo_morphology:
    classification_value: Embryonal Neoplasm
    evidence:
    - reference: PMID:37385707
      reference_title: "Wilms Tumor."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Wilms tumor (WT), or nephroblastoma, is the most common primary malignant renal
        tumor of childhood. It is an embryonal tumor that develops from remnants of
        immature kidney.
      explanation: >-
        Confirms Wilms tumor is an embryonal neoplasm arising from immature kidney.
  harrisons_chapter:
  - classification_value: cancer
    evidence:
    - reference: PMID:37385707
      reference_title: "Wilms Tumor."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Wilms tumor (WT), or nephroblastoma, is the most common primary malignant renal
        tumor of childhood.
      explanation: >-
        Confirms Wilms tumor is a malignant neoplasm classified under cancer.
  - classification_value: solid tumor
    evidence:
    - reference: PMID:33394739
      reference_title: "New approaches to risk stratification for Wilms tumor."
      supports: SUPPORT
      evidence_source: HUMAN_CLINICAL
      snippet: >-
        Wilms tumor (WT) is the second most common extracranial solid tumor and the
        most common malignant renal tumor in children.
      explanation: >-
        Confirms Wilms tumor is classified as a solid tumor.
mappings:
  icd10cm_mappings:
  - term:
      id: ICD10CM:C64.9
      label: Malignant neoplasm of unspecified kidney, except renal pelvis
    mapping_predicate: skos:closeMatch
    mapping_source: ICD-10-CM
    mapping_justification: >-
      ICD-10-CM C64 category covers malignant neoplasms of kidney including
      nephroblastoma/Wilms tumor. C64.9 is used when laterality is unspecified.
      C64.1 (right) and C64.2 (left) specify laterality.
  mondo_mappings:
  - term:
      id: MONDO:0006058
      label: Wilms tumor
    mapping_predicate: skos:exactMatch
    mapping_source: MONDO
    mapping_justification: Primary MONDO disease identifier for this Wilms tumor entry.
  ncit_mappings:
  - term:
      id: NCIT:C3267
      label: Wilms Tumor
    mapping_predicate: skos:exactMatch
    mapping_source: NCIT
    mapping_justification: Primary NCIT disease concept corresponding to Wilms tumor.
datasets:
- accession: dbgap:phs000471
  title: "TARGET: Kidney, Wilms Tumor (WT)"
  description: >-
    TARGET high-risk Wilms tumor cohort with matched tumor-normal profiling,
    including gene expression, copy number, methylation, whole genome sequencing,
    and subset mRNA-seq, miRNA-seq, and whole exome sequencing.
  organism:
    preferred_term: human
    term:
      id: NCBITaxon:9606
      label: Homo sapiens
  data_type: MULTI_OMICS
  sample_types:
  - preferred_term: Wilms tumor tissue
    tissue_term:
      preferred_term: kidney
      term:
        id: UBERON:0002113
        label: kidney
  sample_count: 130
  conditions:
  - high-risk Wilms tumor
  - anaplastic Wilms tumor
  - relapsed favorable histology Wilms tumor
  notes: >-
    dbGaP describes 130 fully characterized high-risk Wilms tumor cases with
    tumor-normal pairs, selected largely from anaplastic tumors or favorable
    histology tumors that relapsed.
clinical_trials:
- name: NCT05985161
  phase: PHASE_II
  description: >-
    Multi-center phase II study of selinexor in recurrent or refractory Wilms
    tumor and other pediatric solid tumors, including a dedicated Wilms tumor
    cohort.
  evidence:
  - reference: clinicaltrials:NCT05985161
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: >-
      The purpose of this study is to find out whether selinexor is an effective
      treatment for people who have a relapsed/refractory Wilms tumor, rhabdoid
      tumor, MPNST, or another solid tumor that makes a higher than normal amount
      of XPO1 or has genetic changes that increase the activity of XP01.
    explanation: >-
      Supports a Wilms-specific phase II selinexor clinical trial for recurrent
      or refractory disease.
📚

References & Deep Research

Deep Research

1
Falcon
Disease Pathophysiology Research Template
Edison Scientific Literature 39 citations 2026-03-28T05:21:02.269146

Question: You are an expert researcher providing comprehensive, well-cited information.

Provide detailed information focusing on: 1. Key concepts and definitions with current understanding 2. Recent developments and latest research (prioritize 2023-2024 sources) 3. Current applications and real-world implementations 4. Expert opinions and analysis from authoritative sources 5. Relevant statistics and data from recent studies

Format as a comprehensive research report with proper citations. Include URLs and publication dates where available. Always prioritize recent, authoritative sources and provide specific citations for all major claims.

Disease Pathophysiology Research Template

Target Disease

  • Disease Name: Wilms Tumor
  • MONDO ID: (if available)
  • Category:

Research Objectives

Please provide a comprehensive research report on the pathophysiology of Wilms Tumor. Focus on the molecular and cellular mechanisms underlying disease progression.

Required Information

1. Core Pathophysiology

  • What are the primary pathophysiological mechanisms?
  • What molecular pathways are dysregulated?
  • What cellular processes are affected?

2. Key Molecular Players

  • Genes/Proteins: Which genes are causally involved or implicated?
  • Chemical Entities: What metabolites, drugs, or small molecules are relevant?
  • Cell Types: Which cell types are primarily affected?
  • Anatomical Locations: Which tissues/organs are involved?

3. Biological Processes (for GO annotation)

  • What biological processes (GO terms) are disrupted?
  • Consider: signaling pathways, metabolic processes, cellular responses, transport, etc.

4. Cellular Components

  • Where in the cell do key processes occur?
  • Consider: organelles, membrane compartments, extracellular space, etc.

5. Disease Progression

  • What is the sequence of events from initial trigger to clinical manifestation?
  • Are there distinct stages or phases?

6. Phenotypic Manifestations

  • What are the key clinical phenotypes?
  • How do they relate to the underlying mechanisms?

Citation Requirements

  • Cite primary literature (PMID preferred) for all mechanistic claims
  • Prioritize recent reviews and landmark papers
  • Include direct quotes where possible to support key statements

Output Format

Structure your response as a narrative that could be used to populate a disease knowledge base entry with: - Pathophysiology description - Gene/protein annotations with ontology terms (HGNC, GO) - Phenotype associations (HP terms) - Cell type involvement (CL terms) - Anatomical locations (UBERON terms) - Chemical entities (CHEBI terms) - Evidence items with PMIDs

Wilms Tumor (Nephroblastoma) Pathophysiology — Molecular and Cellular Mechanisms (2023–2024 emphasis)

Target Disease

  • Disease name: Wilms tumor (nephroblastoma)
  • MONDO ID: MONDO:0006058 (Open Targets disease mapping) (rastegar2026understandinganaplasticwilms pages 77-80)
  • Category: Embryonal pediatric renal malignancy (kidney cancer) arising from disrupted renal development programs with triphasic histology (blastemal, epithelial, stromal) (perotti2024hallmarkdiscoveriesin pages 6-10, tiburcio2024drosharegulatesmesenchymal pages 1-3, neagu2025wilms’tumora pages 6-8)

Executive summary (current understanding)

Wilms tumor is best understood as a developmental cancer in which aberrant fetal kidney programs persist or are reactivated, often via early embryonic events that create nephrogenic rests and/or clonal nephrogenesis, followed by acquisition of additional genetic/epigenetic driver events that lock cells into progenitor-like states and enable malignant growth (perotti2024hallmarkdiscoveriesin pages 6-10, perotti2024hallmarkdiscoveriesin pages 25-29). The dominant mechanistic axes supported by recent synthesis and primary studies include: 11p15 imprinting/IGF2 dysregulation, WT1 loss, WNT/β-catenin activation (CTNNB1; AMER1/WTX), microRNA-processing defects (DROSHA/DGCR8/DICER1), SIX1/SIX2 nephron progenitor dysregulation, and (in high-risk subsets) TP53-driven anaplasia with additional genomic instability, including 1q gain and LOH 1p/16q that are used in risk stratification (perotti2024hallmarkdiscoveriesin pages 10-14, perotti2024hallmarkdiscoveriesin pages 14-17, perotti2024hallmarkdiscoveriesin pages 17-21, cantoni2026tumormicroenvironmentand pages 2-4).

Mechanism/pathway Key genes/proteins (HGNC symbols) Cellular process (GO-style phrase) Cell types/anatomy (CL/UBERON terms as text) Evidence highlights with quantitative stats Key recent sources (author, year, journal) with URL Evidence citation IDs (pqac-...)
11p15 imprinting / IGF2-H19 dysregulation IGF2, H19, KCNQ1OT1, KCNQ1, WT1 genomic imprinting; regulation of IGF signaling; epigenetic regulation of gene expression embryonic renal progenitor / nephrogenic rest-like cells; blastemal tumor cells; kidney (UBERON:kidney), chromosome 11p15.5 locus 11p15 alterations are among the most frequent WT events; IGF2 overexpression occurs in up to 70% of sporadic tumors and is near-universal in blastemal-type tumors. In bilateral WT, 58/99 (58.6%) tumors had H19/ICR1 loss of imprinting and 25/99 (25.2%) had 11p15.5 LOH; 29/30 (96.7%) synchronous tumors were concordant for 11p15.5 status. In 18/19 (94.7%) LOH tumors, breakpoints overlapped WT1/11p13, supporting coupled WT1/IGF2 dysregulation. Perotti et al., 2024, Nat Rev Urol — https://doi.org/10.1038/s41585-023-00824-0; Murphy et al., 2023, Nat Commun — https://doi.org/10.1038/s41467-023-43730-0; Nirgude et al., 2024, Br J Cancer — https://doi.org/10.1038/s41416-023-02538-x (perotti2024hallmarkdiscoveriesin pages 10-14, murphy2023geneticandepigenetic pages 4-5, murphy2023geneticandepigenetic pages 1-2, nirgude2024cancerpredispositionsignaling pages 1-2)
WT1 tumor suppressor / developmental regulator WT1, IGF2, CTNNB1 kidney development; nephron progenitor differentiation; tumor suppressor activity; transcription regulation metanephric mesenchyme / embryonic renal progenitors; nephrogenic rests; kidney (UBERON:kidney) WT1 mutations occur in ~6–20% of sporadic tumors and define a classic predisposition pathway. In bilateral WT, germline WT1 variants were found in 9/61 (14.8%) patients, and all 14 tumors from these patients showed 11p15.5 LOH; CTNNB1 mutations occurred in 10/14 (71.4%) of this WT1-associated set, supporting a stereotyped WT1 → 11p LOH/IGF2 activation → CTNNB1 sequence. Perotti et al., 2024, Nat Rev Urol — https://doi.org/10.1038/s41585-023-00824-0; Murphy et al., 2023, Nat Commun — https://doi.org/10.1038/s41467-023-43730-0 (perotti2024hallmarkdiscoveriesin pages 6-10, murphy2023geneticandepigenetic pages 1-2, murphy2023geneticandepigenetic pages 7-9)
WNT/β-catenin activation CTNNB1, AMER1, WT1 canonical Wnt signaling; β-catenin stabilization; regulation of progenitor proliferation and myogenic differentiation blastemal tumor cells; epithelial/prototubular elements; embryonic kidney (UBERON:kidney) CTNNB1 activating mutations occur in ~15% of WTs; ~65% affect exon 3, causing loss of phosphorylation sites and β-catenin stabilization. There is a strong WT1-CTNNB1 association: 19/20 CTNNB1-mutant tumors also carried WT1 mutations (p = 3.6 × 10^-13). AMER1 inactivation has been reported in up to 30% of tumors in initial studies (additional screens 7–18%). Perotti et al., 2024, Nat Rev Urol — https://doi.org/10.1038/s41585-023-00824-0 (perotti2024hallmarkdiscoveriesin pages 14-17, perotti2024hallmarkdiscoveriesin pages 10-14)
miRNA processing defects and ferroptosis vulnerability DROSHA, DGCR8, DICER1, XPO5, GPX4, ACSL4, CCND2 miRNA biogenesis; post-transcriptional gene silencing; maintenance of mesenchymal progenitor state; regulation of ferroptotic cell death self-renewing mesenchymal/blastemal tumor cells; embryonic renal progenitors; kidney (UBERON:kidney) miRNA-processing gene mutations are enriched in blastemal WT: DROSHA/DGCR8 in ~18.2% of blastemal cases; DROSHA ~10% in favorable-histology WT; DGCR8 ~4.5%. In bilateral WT, DROSHA mutations were seen in 7/85 (8.2%) tumors and DGCR8 in 4/85 (4.7%). DROSHA loss de-represses miRNA targets, increases CCND2, and is associated with ACSL4/redox abnormalities that sensitize cells to GPX4 inhibition and ferroptosis. Tiburcio et al., 2024, Mol Cancer Res — https://doi.org/10.1158/1541-7786.mcr-23-0930; Perotti et al., 2024, Nat Rev Urol — https://doi.org/10.1038/s41585-023-00824-0; Murphy et al., 2023, Nat Commun — https://doi.org/10.1038/s41467-023-43730-0 (tiburcio2024drosharegulatesmesenchymal pages 1-3, perotti2024hallmarkdiscoveriesin pages 17-21, murphy2023geneticandepigenetic pages 4-5)
SIX1/SIX2 progenitor-state program SIX1, SIX2 maintenance of nephron progenitor cell population; regulation of cell cycle; kidney development; adhesion gene regulation blastemal tumor cells; nephron progenitor/cap mesenchyme-like cells; kidney (UBERON:kidney) Recurrent SIX1/SIX2 Q177R homeodomain mutations occur in 18.1% of blastemal cases and 4.3% overall. These are linked to a progenitor-like state, chemotherapy-resistant blastemal tumors, and altered cell-cycle, kidney-development, and adhesion programs. Perotti et al., 2024, Nat Rev Urol — https://doi.org/10.1038/s41585-023-00824-0 (perotti2024hallmarkdiscoveriesin pages 17-21, perotti2024hallmarkdiscoveriesin pages 14-17)
MYCN dysregulation MYCN positive regulation of cell proliferation; transcriptional amplification; oncogenic progenitor expansion blastemal/anaplastic tumor cells; embryonic kidney-derived tumor compartments MYCN gain/amplification and recurrent P44L mutation are described in WT; MYCN gain is associated with anaplastic histology and poor outcome. Reviews classify MYCN activation as one of the major transcriptional/mutational subclasses in WT. Perotti et al., 2024, Nat Rev Urol — https://doi.org/10.1038/s41585-023-00824-0; Tiburcio et al., 2024, Mol Cancer Res — https://doi.org/10.1158/1541-7786.mcr-23-0930 (perotti2024hallmarkdiscoveriesin pages 14-17, tiburcio2024drosharegulatesmesenchymal pages 1-3)
TP53 dysfunction and anaplasia TP53 DNA damage response; apoptotic signaling; anaplastic transformation diffuse anaplastic tumor cells; high-risk Wilms tumor compartments; kidney tumor tissue Diffuse anaplasia occurs in ~5–10% of WTs. TP53 is mutated in ~50–60% of diffuse anaplastic WTs, and TP53 immunopositivity is usually associated with anaplasia and poor prognosis. Perotti et al., 2024, Nat Rev Urol — https://doi.org/10.1038/s41585-023-00824-0 (perotti2024hallmarkdiscoveriesin pages 10-14)
Copy-number risk biomarkers 1q gain; LOH 1p; LOH 16q; chromosome 12 gain chromosomal instability; copy-number alteration-associated progression; relapse risk stratification Wilms tumor tissue broadly; high-risk blastemal and anaplastic compartments Combined LOH at 1p/16q is an established adverse biomarker in COG protocols. 1q gain occurs in ~20–30% of cases and is a strong predictor of poor outcome. In one 2024 genomic/modeling study, 1q gain was seen in 2 patients, combined LOH 1p/16q in 2 patients, and chromosome 12 gain (linked to relapse) in 2 patients. Mittal et al., 2024, Commun Biol — https://doi.org/10.1038/s42003-024-06140-6; Cantoni et al., 2026, Cancers — https://doi.org/10.3390/cancers18060908; Lim & Loh, 2024, Cancers — https://doi.org/10.3390/cancers16173051 (mittal2024targetingtrip13in pages 2-3, cantoni2026tumormicroenvironmentand pages 2-4, lim2024interethnicvariationsin pages 15-17)
XPO1 nuclear export dependency / selinexor vulnerability XPO1, TRIP13 nuclear export; regulation of cell survival; drug response Wilms tumor cell lines and recurrent/refractory Wilms tumor; kidney tumor tissue XPO1 was identified as a vulnerability in WT models; selinexor suppressed TRIP13 and synergized with doxorubicin in vivo/in vitro. A dedicated Phase II trial (NCT05985161) is recruiting for relapsed/refractory or high-risk Wilms tumor and other XPO1-dependent solid tumors; estimated enrollment ~45, with a Wilms-specific cohort and primary endpoint of overall response rate at 6 months. Mittal et al., 2024, Commun Biol — https://doi.org/10.1038/s42003-024-06140-6; ClinicalTrials.gov NCT05985161 (2023) — https://clinicaltrials.gov/study/NCT05985161 (mittal2024targetingtrip13in pages 2-3, NCT05985161 chunk 1, cantoni2026tumormicroenvironmentand pages 19-20)

Table: This table summarizes major molecular mechanisms, genes, cellular processes, anatomic context, and recent evidence for Wilms tumor pathophysiology. It is useful as a compact knowledge-base style overview linking mechanistic biology to quantitative biomarkers and emerging therapeutic vulnerabilities.

1) Key concepts and definitions

1.1 Developmental origin and histology

Wilms tumor typically resembles fetal kidney and often contains a triphasic mixture of (i) blastema (undifferentiated metanephric mesenchyme), (ii) epithelial cells (prototubular/primitive tubules), and (iii) stromal elements (mesenchymal tissues). The blastemal compartment is often considered the aggressive and less-differentiated compartment and can be predominant in monophasic cases (neagu2025wilms’tumora pages 6-8, perotti2024hallmarkdiscoveriesin pages 6-10, tiburcio2024drosharegulatesmesenchymal pages 1-3).

1.2 Nephrogenic rests and clonal nephrogenesis

Wilms tumors frequently arise within or adjacent to nephrogenic rests (embryonic renal precursor remnants) (perotti2024hallmarkdiscoveriesin pages 6-10, tiburcio2024drosharegulatesmesenchymal pages 1-3). Perotti et al. (Nature Reviews Urology; online 2023, issue Oct 2024) further highlight that intralobar nephrogenic rests (ILNR) and perilobar nephrogenic rests (PLNR) associate with distinct molecular subsets; PLNR is enriched in tumor subsets with dysregulated nephrogenic-zone self-renewal/differentiation programs (perotti2024hallmarkdiscoveriesin pages 25-29).

1.3 Genomic imprinting in Wilms tumor

Perotti et al. explicitly define genomic imprinting as parent-specific monoallelic expression and describe Wilms tumor–predisposing lesions at 11p15 affecting imprint control regions for IGF2/H19 (perotti2024hallmarkdiscoveriesin pages 6-10, perotti2024hallmarkdiscoveriesin pages 10-14).

2) Core pathophysiology: dysregulated pathways and cellular processes

2.1 11p15 imprinting defects → IGF2-driven growth signaling

A major early and recurrent driver is abnormal imprinting/allelic imbalance at 11p15 (WT2 region), affecting IGF2 (growth factor) and H19. Perotti et al. report IGF2 overexpression in up to ~70% of sporadic tumors and its presence in nephrogenic rests, supporting an early lesion that precedes frank malignancy in many cases (perotti2024hallmarkdiscoveriesin pages 10-14).

Recent 2023–2024 developments (bilateral predisposition and mosaicism): Murphy et al. (Nature Communications, Dec 2023; https://doi.org/10.1038/s41467-023-43730-0) provide strong evidence that bilateral disease frequently reflects shared early 11p15.5 epigenetic/copy-number events rather than a shared tumor clone. Across 99 bilateral tumor specimens, they report 11p15.5 LOI (H19/ICR1 hypermethylation) in 58/99 (58.6%), 11p15.5 LOH in 25/99 (25.2%), and normal imprinting retention in 16/99 (16.1%) (murphy2023geneticandepigenetic pages 1-2). Synchronous paired tumors were concordant for 11p15.5 status in 29/30 (96.7%) yet usually lacked shared somatic variants, supporting independent tumor origins seeded by early predisposition events (murphy2023geneticandepigenetic pages 4-5).

In Beckwith–Wiedemann syndrome (BWS), Nirgude et al. (British Journal of Cancer, Dec 2024; https://doi.org/10.1038/s41416-023-02538-x) describe mosaic 11p15 LOH due to paternal uniparental isodisomy and note that LOH at 11p15 is present in at least 30% of Wilms tumors and is associated with higher staging (nirgude2024cancerpredispositionsignaling pages 1-2).

Cellular processes impacted: growth factor signaling, epigenetic regulation, cell proliferation and survival, and maintenance of embryonic progenitor programs (perotti2024hallmarkdiscoveriesin pages 10-14, nirgude2024cancerpredispositionsignaling pages 1-2).

2.2 WT1 tumor suppressor loss and aberrant nephrogenesis

WT1 is a master developmental regulator in kidney organogenesis. Perotti et al. summarize WT1’s role in mesenchymal–epithelial transitions and note that Wilms tumors often arise adjacent to nephrogenic rests, consistent with early developmental disruption (perotti2024hallmarkdiscoveriesin pages 6-10). Murphy et al. report that WT1 germline variants were among the most common detectable predispositions in their bilateral cohort (9/61, 14.8%) (murphy2023geneticandepigenetic pages 1-2).

A key mechanistic pattern in WT1-predisposed disease is a stereotyped evolutionary sequence in which early 11p events both (i) drive biallelic WT1 inactivation and (ii) activate the IGF2 imprinting domain, followed by WNT pathway activation. Murphy et al. report that in WT1-predisposed tumors, 11p15.5 LOH was present in all tumors (14/14) and CTNNB1 exon 3 activating mutations occurred in 10/14 (71.4%) of the WT1-associated set (murphy2023geneticandepigenetic pages 1-2).

2.3 WNT/β-catenin signaling (CTNNB1) and β-catenin destruction complex (AMER1/WTX)

Perotti et al. report CTNNB1 activating mutations in ~15% of Wilms tumors, with many affecting exon 3 to stabilize β-catenin and activate canonical WNT signaling. They also report a very strong association between WT1 and CTNNB1 mutations: 19/20 CTNNB1-mutant tumors also had WT1 mutations (p = 3.6 × 10−13) (perotti2024hallmarkdiscoveriesin pages 14-17). AMER1 (WTX) is described as part of the β-catenin destruction complex, with inactivation reported in up to ~30% in early screens (perotti2024hallmarkdiscoveriesin pages 14-17).

Clinical/biologic implication: this axis connects renal development programs to sustained progenitor proliferation and lineage mis-specification (perotti2024hallmarkdiscoveriesin pages 14-17).

2.4 MicroRNA-processing defects (DROSHA/DGCR8/DICER1) maintain progenitor/mesenchymal state and create metabolic vulnerabilities

A key modern concept is that a substantial subset of Wilms tumors are driven by disruption of miRNA biogenesis, which derepresses miRNA target programs controlling differentiation and proliferation.

  • Perotti et al. report recurrent miRNA-processing pathway mutations in DROSHA, DGCR8, and DICER1, enriched in blastemal tumors; they cite DROSHA/DGCR8 in ~18.2% of blastemal cases and note DROSHA ~10% in favorable-histology tumors and DGCR8 ~4.5% (perotti2024hallmarkdiscoveriesin pages 17-21).
  • Murphy et al. quantify these alterations in bilateral tumors (WES/WGS panel): DROSHA 7/85 (8.2%) and DGCR8 4/85 (4.7%), and report pathway enrichment for RNA/miRNA biogenesis, p53, and transcription pathways (murphy2023geneticandepigenetic pages 4-5).

Mechanistic primary evidence (2024): Tiburcio et al. (Molecular Cancer Research, Apr 2024; https://doi.org/10.1158/1541-7786.mcr-23-0930) show that DROSHA mutations correlate with derepression of miRNA target genes and a self-renewing mesenchymal state. They also connect miRNA loss to redox/lipid biology: miRNA-deficient Wilms tumor cells show aberrant redox metabolism and are sensitized to ferroptotic cell death through inhibition of GPX4, which detoxifies lipid peroxides (tiburcio2024drosharegulatesmesenchymal pages 1-3).

2.5 SIX1/SIX2 (nephron progenitor program) and blastemal chemo-resistance

Perotti et al. report recurrent SIX1/SIX2 Q177R mutations in the homeodomain, frequent in blastemal tumors (18.1% of blastemal cases; 4.3% overall) and associated with a progenitor-like state and chemotherapy-resistant blastemal tumors (perotti2024hallmarkdiscoveriesin pages 17-21).

2.6 MYCN activation and high-risk biology

Perotti et al. describe MYCN gain/amplification and recurrent MYCN mutations (e.g., P44L), and specifically note that MYCN gain is associated with anaplastic histology and poor outcome (perotti2024hallmarkdiscoveriesin pages 14-17).

2.7 TP53-driven anaplasia (high-risk subtype)

Perotti et al. report that diffuse anaplasia occurs in ~5–10% of Wilms tumors and that TP53 is mutated in ~50–60% of diffuse anaplastic Wilms tumors; they further note that TP53 immunopositivity is used as a surrogate for mutation and correlates with poor prognosis (perotti2024hallmarkdiscoveriesin pages 10-14).

3) Key molecular players (genes/proteins, chemical entities, cell types, anatomy)

3.1 Genes/proteins (HGNC symbols; selected)

  • Tumor suppressors / developmental regulators: WT1; TP53 (anaplasia); REST; TRIM28; CTR9 (PAF1 complex) (perotti2024hallmarkdiscoveriesin pages 21-25, perotti2024hallmarkdiscoveriesin pages 10-14, rastegar2026understandinganaplasticwilmsa pages 38-42)
  • Imprinting/growth signaling: IGF2; H19; KCNQ1OT1; KCNQ1 (perotti2024hallmarkdiscoveriesin pages 10-14, nirgude2024cancerpredispositionsignaling pages 1-2, murphy2023geneticandepigenetic pages 6-7)
  • WNT/β-catenin: CTNNB1; AMER1/WTX (perotti2024hallmarkdiscoveriesin pages 14-17)
  • miRNA biogenesis: DROSHA; DGCR8; DICER1; XPO5 (perotti2024hallmarkdiscoveriesin pages 17-21, murphy2023geneticandepigenetic pages 4-5)
  • Nephron progenitor/renal development transcription factors: SIX1; SIX2; OSR1; HOXA11 (perotti2024hallmarkdiscoveriesin pages 25-29, perotti2024hallmarkdiscoveriesin pages 17-21)
  • Oncogenic transcriptional amplifiers: MYCN (perotti2024hallmarkdiscoveriesin pages 14-17)
  • Nuclear export dependency: XPO1; downstream TRIP13 (mittal2024targetingtrip13in pages 2-3)

3.2 Chemical entities / small molecules (CHEBI-style; named entities)

Evidence in the retrieved corpus names drug-like agents (not formal CHEBI IDs): - Selinexor (KPT-330), an XPO1 inhibitor, tested in Wilms tumor (NCT05985161) (NCT05985161 chunk 1, cantoni2026tumormicroenvironmentand pages 19-20) - Tegavivint, described as a WNT/β-catenin inhibitor in pediatric trials (NCT04851119) (cantoni2026tumormicroenvironmentand pages 19-20) - Doxorubicin, used clinically and shown to synergize with nuclear export inhibition in a Wilms model system (mittal2024targetingtrip13in pages 2-3) - GPX4 inhibitors (e.g., discussed mechanistically as GPX4 inhibition) to induce ferroptosis in miRNA-deficient subset (tiburcio2024drosharegulatesmesenchymal pages 1-3)

3.3 Cell types (CL terms as text) and microenvironment

  • Nephron progenitor/cap mesenchyme-like cells; embryonic renal progenitors (developmental origin) (perotti2024hallmarkdiscoveriesin pages 25-29, tiburcio2024drosharegulatesmesenchymal pages 1-3)
  • Tumor compartments: blastemal cells, epithelial prototubules/tubular cells, stromal/fibroblast-like cells (perotti2024hallmarkdiscoveriesin pages 6-10, perotti2024hallmarkdiscoveriesin pages 25-29)
  • Immune cells: tumor-associated macrophages (TAMs; CD68+), skewed toward M2-like macrophages (Arg-1, CD163, CD206); neutrophils; mast cells; Tregs; low CD8+ T cells (cantoni2026tumormicroenvironmentand pages 6-7, rastegar2026understandinganaplasticwilmsa pages 38-42)

3.4 Anatomical locations (UBERON terms as text)

  • Kidney (pediatric renal tumor)
  • Nephrogenic rests (intralobar/perilobar) in kidney (perotti2024hallmarkdiscoveriesin pages 25-29, perotti2024hallmarkdiscoveriesin pages 6-10)
  • Chromosome 11p15.5 imprinting domain; 11p13 WT1 locus (perotti2024hallmarkdiscoveriesin pages 10-14, murphy2023geneticandepigenetic pages 4-5)

4) Biological processes (GO annotation candidates)

The following disrupted processes are directly supported by the evidence base above: - Genomic imprinting / epigenetic regulation of gene expression (11p15.5; H19/ICR1 methylation) (perotti2024hallmarkdiscoveriesin pages 10-14, murphy2023geneticandepigenetic pages 1-2) - Regulation of growth factor signaling (IGF2 axis) (perotti2024hallmarkdiscoveriesin pages 10-14) - Kidney development / nephron progenitor differentiation; mesenchymal–epithelial transition (WT1, SIX programs; fetal kidney mapping) (perotti2024hallmarkdiscoveriesin pages 6-10, perotti2024hallmarkdiscoveriesin pages 25-29) - Canonical WNT signaling (β-catenin stabilization from CTNNB1 exon 3 mutations; AMER1/WTX disruption) (perotti2024hallmarkdiscoveriesin pages 14-17) - miRNA-mediated gene silencing / miRNA biogenesis (DROSHA/DGCR8/DICER1) (perotti2024hallmarkdiscoveriesin pages 17-21, murphy2023geneticandepigenetic pages 4-5) - Ferroptotic cell death / lipid peroxide detoxification (GPX4 dependence in miRNA-deficient state) (tiburcio2024drosharegulatesmesenchymal pages 1-3) - DNA damage response / apoptotic signaling (TP53 dysfunction in diffuse anaplasia) (perotti2024hallmarkdiscoveriesin pages 10-14) - Immune evasion / macrophage polarization (M2-skewed TAMs; immune-cold features) (cantoni2026tumormicroenvironmentand pages 6-7, rastegar2026understandinganaplasticwilmsa pages 38-42)

5) Cellular components (cellular anatomy; GO-CC candidates)

  • Nucleus (WT1 transcription/RNA metabolism roles; REST/TRIM28/CTR9 transcriptional regulation) (perotti2024hallmarkdiscoveriesin pages 6-10, perotti2024hallmarkdiscoveriesin pages 21-25)
  • miRNA microprocessor machinery (functionally represented by DROSHA/DGCR8; plus DICER1 in cytoplasmic processing pathway) (perotti2024hallmarkdiscoveriesin pages 17-21, murphy2023geneticandepigenetic pages 4-5)
  • Plasma membrane and lipid peroxidation sites relevant to ferroptosis (ACSL4-mediated PUFA incorporation; GPX4 detoxification of lipid peroxides) (tiburcio2024drosharegulatesmesenchymal pages 1-3)

6) Disease progression model (sequence of events)

A mechanistically supported progression model (not necessarily universal across all patients) is: 1. Early developmental lesion in fetal kidney lineages leading to nephrogenic rests and/or clonal nephrogenesis (perotti2024hallmarkdiscoveriesin pages 6-10, perotti2024hallmarkdiscoveriesin pages 25-29). 2. Initiating epigenetic/copy-number event at 11p15.5 (LOI or LOH) resulting in increased IGF2 expression and altered imprinting balance; this is especially prominent in bilateral predisposition and BWS-associated cases (murphy2023geneticandepigenetic pages 1-2, nirgude2024cancerpredispositionsignaling pages 1-2). 3. Acquisition of additional driver mutations that enforce progenitor-like programs and proliferation: - WT1 inactivation and/or WNT activation (CTNNB1; AMER1/WTX) (perotti2024hallmarkdiscoveriesin pages 14-17, murphy2023geneticandepigenetic pages 1-2). - miRNA-processing loss (DROSHA/DGCR8/DICER1) causing widespread derepression of differentiation/proliferation programs and metabolic rewiring (tiburcio2024drosharegulatesmesenchymal pages 1-3, perotti2024hallmarkdiscoveriesin pages 17-21). - SIX1/SIX2 mutations sustaining nephron progenitor states and chemotherapy resistance in blastemal tumors (perotti2024hallmarkdiscoveriesin pages 17-21). 4. Progression to high-risk phenotypes via genomic instability and additional lesions: - TP53 disruption associated with diffuse anaplasia (5–10% of tumors; TP53 mutated ~50–60% in diffuse anaplasia) (perotti2024hallmarkdiscoveriesin pages 10-14). - Copy-number alterations including 1q gain and LOH 1p/16q, used for risk assessment and associated with relapse/poor outcome (cantoni2026tumormicroenvironmentand pages 2-4, mittal2024targetingtrip13in pages 2-3).

7) Phenotypic manifestations (HP term candidates; clinical-pathologic links)

The following phenotype elements are supported conceptually and/or explicitly described in the retrieved evidence: - Renal neoplasm / embryonal tumor of kidney (disease entity) - Triphasic histology: blastemal, epithelial, stromal components; blastemal predominance as aggressive phenotype (neagu2025wilms’tumora pages 6-8, perotti2024hallmarkdiscoveriesin pages 6-10) - Nephrogenic rests (preneoplastic lesions), including ILNR/PLNR; association of PLNR with specific molecular subsets (perotti2024hallmarkdiscoveriesin pages 25-29, tiburcio2024drosharegulatesmesenchymal pages 1-3) - Diffuse anaplasia: nuclear enlargement/hyperchromasia and atypical multipolar mitoses; poor prognosis and TP53 association (perotti2024hallmarkdiscoveriesin pages 10-14, rastegar2026understandinganaplasticwilmsa pages 38-42)

8) Recent developments and latest research (prioritizing 2023–2024)

8.1 2023: Quantitative predisposition architecture in bilateral Wilms tumor (COG AREN18B5-Q)

Murphy et al. (Nature Communications, Dec 2023; https://doi.org/10.1038/s41467-023-43730-0) provide a modern framework in which bilateral disease is commonly driven by either detectable germline predisposition variants or post-zygotic mosaic imprinting defects at 11p15.5. Key statistics include LOI/LOH/ROI proportions across 99 tumors and high concordance of 11p15.5 status across synchronous tumors (murphy2023geneticandepigenetic pages 1-2, murphy2023geneticandepigenetic pages 4-5).

8.2 2024: miRNA-processing subclass mechanistically linked to ferroptosis sensitivity

Tiburcio et al. (Molecular Cancer Research, Apr 2024; https://doi.org/10.1158/1541-7786.mcr-23-0930) move beyond association to mechanism by showing DROSHA-driven transcriptional programs and demonstrating a therapeutic vulnerability: miRNA-deficient Wilms cells are sensitized to ferroptosis through GPX4 inhibition (tiburcio2024drosharegulatesmesenchymal pages 1-3).

8.3 2024: Consolidation of “hallmark” pathways and quantification of key high-risk features

Perotti et al. (Nature Reviews Urology; Oct 2024 issue; https://doi.org/10.1038/s41585-023-00824-0) synthesize the field with key quantitative statements that directly support knowledge-base assertions, including (i) IGF2 overexpression up to ~70% of sporadic tumors, (ii) CTNNB1 frequency (~15%) and tight association with WT1, (iii) SIX1/2 Q177R enrichment in blastemal tumors, and (iv) TP53 mutation frequency (~50–60%) in diffuse anaplasia (perotti2024hallmarkdiscoveriesin pages 10-14, perotti2024hallmarkdiscoveriesin pages 14-17, perotti2024hallmarkdiscoveriesin pages 17-21).

8.4 2024: Translational vulnerability—nuclear export (XPO1) and patient-derived models

Mittal et al. (Communications Biology, Apr 2024; https://doi.org/10.1038/s42003-024-06140-6) report clinically relevant genomic biomarkers in a small cohort (e.g., 1q gain, LOH 1p/16q) and identify XPO1 as a vulnerability, with nuclear export inhibitors synergizing with doxorubicin in vivo (mittal2024targetingtrip13in pages 2-3).

9) Current applications and real-world implementations

9.1 Molecular risk stratification and clinical decision-making

Evidence in the retrieved set supports several widely used stratification features: - Diffuse anaplasia and TP53: Diffuse anaplasia is a high-risk subtype (~5–10%); TP53 is mutated in ~50–60% of diffuse anaplastic tumors, and TP53 IHC is used as a surrogate associated with poor prognosis (perotti2024hallmarkdiscoveriesin pages 10-14). - 11p15 status: LOH/LOI at 11p15 is common; in BWS-associated analysis, 11p15 LOH is present in at least 30% of Wilms tumors and associated with higher staging (nirgude2024cancerpredispositionsignaling pages 1-2). - Copy-number biomarkers: combined LOH 1p/16q and 1q gain (20–30% overall) are adverse features; 1q gain is explicitly described as a poor prognostic factor in a 2024 cohort study (cantoni2026tumormicroenvironmentand pages 2-4, mittal2024targetingtrip13in pages 2-3).

9.2 Precision diagnostics and surveillance in predisposition

Murphy et al. show that post-zygotic mosaic 11p15.5 LOI can be detectable in non-tumor tissues (adjacent kidney; sometimes peripheral blood), emphasizing the need for multi-tissue testing strategies when evaluating predisposition in bilateral disease (murphy2023geneticandepigenetic pages 7-9, murphy2023geneticandepigenetic pages 5-6).

9.3 Targeted/experimental therapies and trials

  • XPO1 inhibitor selinexor (ClinicalTrials.gov): NCT05985161 (registered 2023; https://clinicaltrials.gov/study/NCT05985161) is a Phase II study in relapsed/refractory Wilms tumor and other pediatric solid tumors. It includes a Wilms tumor cohort, uses weekly oral selinexor, and has a primary endpoint of overall response rate evaluated at 6 months (NCT05985161 chunk 1).
  • WNT/β-catenin inhibitor tegavivint: A WNT/β-catenin inhibitor (Tegavivint; NCT04851119) is noted as investigational in pediatric trials (cantoni2026tumormicroenvironmentand pages 19-20).
  • IGF axis targeting: Despite IGF2 prevalence, Perotti et al. state that preclinical models and a phase 2 study of IGF-1R blockade were not effective, suggesting pathway redundancy/bypass and the need for combination strategies (perotti2024hallmarkdiscoveriesin pages 10-14).
  • Immunotherapy direction: Multi-tumor-associated antigen T cells targeting WT1/PRAME/survivin have been tested and reported safe with prolonged time-to-progression in early clinical experience; immune checkpoint inhibitors have had limited efficacy in unselected populations, consistent with an immune-cold phenotype (cantoni2026tumormicroenvironmentand pages 19-20, rastegar2026understandinganaplasticwilmsa pages 38-42).

10) Relevant statistics and data points (recent sources)

  • Bilateral predisposition tumor 11p15.5 states (Murphy 2023): LOI 58/99 (58.6%); LOH 25/99 (25.2%); ROI 16/99 (16.1%); concordance across paired synchronous tumors 29/30 (96.7%) (murphy2023geneticandepigenetic pages 1-2, murphy2023geneticandepigenetic pages 4-5).
  • WT1 predisposition frequency in bilateral cohort: WT1 germline variants 9/61 (14.8%) (murphy2023geneticandepigenetic pages 1-2).
  • WT1-associated progression data: 11p15.5 LOH in all WT1-predisposed tumors analyzed (14/14); CTNNB1 exon 3 mutations 10/14 (71.4%) in WT1-associated set (murphy2023geneticandepigenetic pages 1-2).
  • Diffuse anaplasia and TP53: diffuse anaplasia 5–10%; TP53 mutated ~50–60% in diffuse anaplastic tumors (perotti2024hallmarkdiscoveriesin pages 10-14).
  • Risk CNAs: 1q gain present in ~20–30% (review-level estimate) and is a robust predictor of poor outcome; LOH 1p/16q adverse (cantoni2026tumormicroenvironmentand pages 2-4).

11) Expert opinions / authoritative synthesis (with direct quotes)

From Perotti et al. (Nature Reviews Urology; Oct 2024 issue): - On origin: “Modern molecular findings support the embryonic renal origins of Wilms tumours.” (perotti2024hallmarkdiscoveriesin pages 21-25) - On tumor composition: Wilms tumors include “undifferentiated metanephric mesenchyme (blastema) and also more differentiated cells (stroma and tubular epithelial cells normally derived from metanephric mesenchyme)” (perotti2024hallmarkdiscoveriesin pages 21-25).

These statements support the mainstream interpretation that Wilms tumor pathophysiology is primarily a failure of normal renal differentiation programs, with recurrent lesions targeting developmental transcriptional/epigenetic and post-transcriptional (miRNA) regulators (perotti2024hallmarkdiscoveriesin pages 21-25, perotti2024hallmarkdiscoveriesin pages 17-21).

12) Knowledge-base style annotations (ontology-ready; evidence-linked)

12.1 Pathophysiology description (narrative)

Wilms tumor arises when embryonic renal precursor cells (often within nephrogenic rests) experience early epigenetic/copy-number alterations—especially at 11p15.5—leading to dysregulated imprinting (IGF2 overexpression; H19 perturbation) and a growth-promoting, developmentally arrested state. Subsequent driver events (WT1 loss; CTNNB1/AMER1-mediated WNT activation; miRNA-processing defects; SIX1/SIX2 progenitor-program activation) reinforce progenitor self-renewal, impair differentiation, and remodel cellular metabolism. In a subset, additional genomic instability and TP53 defects drive diffuse anaplasia and therapy resistance. The tumor microenvironment is characterized by an inflammatory stromal compartment with M2-like macrophage dominance and generally low cytotoxic T-cell infiltration, contributing to immune evasion (perotti2024hallmarkdiscoveriesin pages 25-29, perotti2024hallmarkdiscoveriesin pages 10-14, perotti2024hallmarkdiscoveriesin pages 14-17, tiburcio2024drosharegulatesmesenchymal pages 1-3, cantoni2026tumormicroenvironmentand pages 6-7, rastegar2026understandinganaplasticwilmsa pages 38-42).

12.2 Gene/protein annotations (examples)

  • WT1 (HGNC:12796) — kidney development; tumor suppressor; transcription/RNA regulation; associated with WT1→11p LOH→CTNNB1 evolutionary path in predisposed bilateral WT (murphy2023geneticandepigenetic pages 1-2, perotti2024hallmarkdiscoveriesin pages 6-10).
  • CTNNB1 (HGNC:2514) — canonical WNT signaling activation (exon 3 stabilization); strongly co-mutates with WT1 (perotti2024hallmarkdiscoveriesin pages 14-17).
  • IGF2 (HGNC:5466) / H19 (HGNC:4713) — imprinting dysregulation at 11p15 with IGF2 overexpression up to ~70% of sporadic tumors (perotti2024hallmarkdiscoveriesin pages 10-14).
  • DROSHA (HGNC:17179) / DGCR8 (HGNC:17233) / DICER1 (HGNC:17098) — miRNA biogenesis defects; linked to mesenchymal progenitor state and GPX4-dependent ferroptosis vulnerability (perotti2024hallmarkdiscoveriesin pages 17-21, tiburcio2024drosharegulatesmesenchymal pages 1-3).
  • SIX1/SIX2 (HGNC:10888/10889) — nephron progenitor program; Q177R enrichment in blastemal and chemo-resistant tumors (perotti2024hallmarkdiscoveriesin pages 17-21).
  • TP53 (HGNC:11998) — diffuse anaplasia driver; mutation ~50–60% of diffuse anaplastic tumors (perotti2024hallmarkdiscoveriesin pages 10-14).

12.3 Cell type involvement (examples)

  • Nephron progenitor / cap mesenchyme-like tumor cells (developmental origin) (perotti2024hallmarkdiscoveriesin pages 25-29, tiburcio2024drosharegulatesmesenchymal pages 1-3)
  • Tumor-associated macrophages (M2-like) in stromal compartment; neutrophils; mast cells; Tregs; low CD8+ T cells (cantoni2026tumormicroenvironmentand pages 6-7, rastegar2026understandinganaplasticwilmsa pages 38-42)

12.4 Anatomical locations (examples)

  • Kidney; nephrogenic rests (ILNR/PLNR) (perotti2024hallmarkdiscoveriesin pages 25-29, perotti2024hallmarkdiscoveriesin pages 6-10)
  • Chromosome 11p15.5 imprinting locus; 11p13 WT1 locus (murphy2023geneticandepigenetic pages 4-5, perotti2024hallmarkdiscoveriesin pages 10-14)

12.5 Evidence items (IDs; publication info)

  • Perotti et al. “Hallmark discoveries in the biology of Wilms tumour.” Nature Reviews Urology (Oct 2024 issue). DOI: https://doi.org/10.1038/s41585-023-00824-0 (perotti2024hallmarkdiscoveriesin pages 10-14, perotti2024hallmarkdiscoveriesin pages 14-17, perotti2024hallmarkdiscoveriesin pages 17-21, perotti2024hallmarkdiscoveriesin pages 25-29)
  • Murphy et al. “Genetic and epigenetic features of bilateral Wilms tumor predisposition…” Nature Communications (Dec 2023). DOI: https://doi.org/10.1038/s41467-023-43730-0 (murphy2023geneticandepigenetic pages 1-2, murphy2023geneticandepigenetic pages 4-5)
  • Tiburcio et al. “DROSHA regulates mesenchymal gene expression in Wilms tumor.” Molecular Cancer Research (Apr 2024). DOI: https://doi.org/10.1158/1541-7786.mcr-23-0930 (tiburcio2024drosharegulatesmesenchymal pages 1-3)
  • Nirgude et al. “Cancer predisposition signaling in Beckwith-Wiedemann Syndrome drives Wilms tumor development.” British Journal of Cancer (Dec 2024). DOI: https://doi.org/10.1038/s41416-023-02538-x (nirgude2024cancerpredispositionsignaling pages 1-2)
  • Mittal et al. “Targeting TRIP13… nuclear export inhibitors…” Communications Biology (Apr 2024). DOI: https://doi.org/10.1038/s42003-024-06140-6 (mittal2024targetingtrip13in pages 2-3)
  • ClinicalTrials.gov NCT05985161 (registered 2023): https://clinicaltrials.gov/study/NCT05985161 (NCT05985161 chunk 1)

Notes on PMID availability

The retrieved evidence snippets did not include PubMed IDs explicitly for the 2023–2024 core sources; therefore, this report cites DOIs and ClinicalTrials.gov identifiers as stable primary references.

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