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0
Mappings
0
Definitions
0
Inheritance
8
Pathophysiology
1
Histopathology
4
Phenotypes
3
Pathograph
9
Genes
4
Treatments
4
Subtypes
0
Differentials
0
Datasets
1
Trials
0
Models
🏷

Classifications

Harrison's Chapter
cancer solid tumor
ICD-O Morphology
Glioma

Subtypes

4
Astrocytoma, IDH-mutant (adult-type, grade 2-4) MONDO:0956994
Adult-type diffuse astrocytic glioma defined by IDH1 or IDH2 mutation and absence of 1p/19q codeletion. WHO CNS5 graded 2-4 based on histology (mitoses, microvascular proliferation, necrosis) and CDKN2A/B homozygous deletion. Curated in detail under IDH_Mutant_Astrocytoma.yaml.
Show evidence (1 reference)
PMID:26061751 SUPPORT Human Clinical
"three robust, nonoverlapping, prognostically significant subtypes of lower-grade glioma that were captured more accurately by IDH, 1p/19q, and TP53 status than by histologic class"
TCGA defines IDH-mutant astrocytic glioma (IDH-mutant, 1p/19q-intact, TP53-mutant) as one of three molecular subtypes that supersede histology, supporting the IDH-mutant subtype assignment.
Diffuse midline glioma, H3 K27-altered (paediatric-type, grade 4) MONDO:1060171
Highly aggressive paediatric-predominant midline glioma defined by H3 K27M mutation (H3F3A/HIST1H3B/C), EZHIP overexpression, or other mechanisms causing global H3K27me3 loss. Universally WHO grade 4. Curated in detail under H3_K27_Altered_Diffuse_Midline_Glioma.yaml.
Show evidence (1 reference)
PMID:35169084 SUPPORT Human Clinical
"Diffuse midline glioma(DMG), H3 K27M-mutant is an infiltrative midline high-grade glioma with predominantly astrocytic differentiation and a K27M mutation in either H3F3A or HIST1H3B/C."
Saito 2022 review supports the DMG H3 K27-altered subtype definition with astrocytic differentiation, midline location, and K27M mutation in H3F3A or HIST1H3B/C.
Diffuse hemispheric glioma, H3 G34-mutant
Hemispheric high-grade glioma of adolescents and young adults driven by glycine-to-arginine or glycine-to-valine substitution at H3F3A position G34. G34R/V mutations alter local chromatin and frequently co-occur with ATRX and TP53 loss. WHO grade 4.
Show evidence (1 reference)
PMID:23079654 SUPPORT Human Clinical
"the two H3F3A mutations give rise to GBMs in separate anatomic compartments"
Sturm et al. show H3F3A K27M and G34 mutations occupy separate anatomic compartments (midline vs hemispheric), supporting DHG H3 G34-mutant as a distinct hemispheric subtype.
Diffuse paediatric-type high-grade glioma, H3-wildtype and IDH-wildtype
Paediatric high-grade diffuse glioma lacking IDH and histone H3 driver mutations. Heterogeneous molecular subgroups defined by methylation profiling (RTK1, RTK2, MYCN). Aggressive course with poor prognosis.
Show evidence (1 reference)
PMID:34185076 SUPPORT Human Clinical
"New tumor types and subtypes are introduced, some based on novel diagnostic technologies such as DNA methylome profiling."
WHO CNS5 introduces methylation-profiled subtypes including diffuse paediatric-type high-grade glioma H3-wildtype/IDH-wildtype, supporting recognition of this subtype.

Pathophysiology

8
Diffuse Parenchymal Infiltration
The defining biological behaviour of all diffuse astrocytomas is single-cell infiltration of brain parenchyma along white-matter tracts and perineuronal spaces, in contrast with the circumscribed growth of pilocytic and other non-diffuse gliomas. This invasive pattern precludes complete surgical resection and underlies recurrence at the resection margin or at distant sites.
astrocyte link
brain link
Show evidence (1 reference)
PMID:34185076 SUPPORT Human Clinical
"the 2021 fifth edition introduces major changes that advance the role of molecular diagnostics in CNS tumor classification."
WHO CNS5 reframes diffuse astrocytoma as a molecularly defined umbrella entity rather than a single morphologic grade-2 lesion, supporting the diffuse-infiltration descriptor across subtypes.
IDH1/2 Neomorphic Mutation and 2-HG Accumulation
In the adult-type IDH-mutant subtype, heterozygous mutations in IDH1 (R132H most common) or IDH2 (R172) confer neomorphic activity, converting alpha-ketoglutarate to the oncometabolite D-2-hydroxyglutarate (2-HG). 2-HG accumulates to millimolar concentrations and competitively inhibits alpha-ketoglutarate-dependent dioxygenases.
astrocyte link
tricarboxylic acid cycle link ⚠ ABNORMAL
Show evidence (1 reference)
PMID:40916936 SUPPORT Human Clinical
"This unique immune microenvironment is shaped by 2-hydroxyglutarate (2-HG), an oncometabolite produced by mutant IDH."
Confirms 2-HG as the oncometabolite produced by mutant IDH in gliomas, supporting the neomorphic enzyme activity described in this mechanism.
G-CIMP Hypermethylation and Blocked Differentiation
Inhibition of TET2 (DNA demethylase) and Jumonji-domain histone demethylases by 2-HG produces the glioma CpG island methylator phenotype (G-CIMP) with global DNA and histone hypermethylation. The resulting transcriptional reprogramming blocks normal glial differentiation, sustaining cells in a proliferative progenitor-like state.
DNA methylation link ↑ INCREASED glial cell differentiation link ↓ DECREASED
Show evidence (1 reference)
PMID:22343889 SUPPORT In Vitro
"mutation of a single gene, isocitrate dehydrogenase 1 (IDH1), establishes G-CIMP by remodelling the methylome."
Turcan et al. demonstrate in primary human astrocytes that mutant IDH1 is sufficient to establish the glioma CpG island methylator phenotype, directly supporting the G-CIMP mechanism downstream of 2-HG.
ATRX/TP53 Cooperative Loss
In the adult IDH-mutant astrocytic lineage, ATRX loss-of-function mutations co-occur with TP53 mutations at very high frequency (approximately 86% ATRX and 94% TP53 in IDH-mutant non-codeleted lower-grade gliomas), and are mutually exclusive with 1p/19q codeletion. ATRX loss licenses alternative lengthening of telomeres (ALT); TP53 loss removes a key tumour-suppressor checkpoint. The ATRX-mutant / TP53-mutant / 1p19q-intact triad operationally defines astrocytic versus oligodendroglial differentiation in IDH-mutant diffuse glioma.
astrocyte link
Show evidence (1 reference)
PMID:26061751 SUPPORT Human Clinical
"Nearly all lower-grade gliomas with IDH mutations and no 1p/19q codeletion had mutations in TP53 (94%) and ATRX inactivation (86%)."
TCGA cohort directly quantifies the ATRX/TP53 cooperative loss in IDH-mutant non-codeleted (astrocytic-lineage) diffuse glioma.
H3 K27M Oncohistone Dominant-Negative PRC2 Inhibition
In paediatric-type midline diffuse astrocytomas, the H3 K27M substitution in H3F3A or HIST1H3B/C generates an oncohistone that, although present in only a minority of total H3 molecules, sequesters and inhibits PRC2/EZH2. Global loss of H3K27 trimethylation derepresses developmental and proliferative programmes, driving aggressive growth in midline structures (pons, thalamus, spinal cord).
astrocyte link
histone H3K27 methyltransferase activity link ↓ DECREASED
pons link
Show evidence (1 reference)
PMID:35169084 SUPPORT Human Clinical
"Diffuse midline glioma(DMG), H3 K27M-mutant is an infiltrative midline high-grade glioma with predominantly astrocytic differentiation and a K27M mutation in either H3F3A or HIST1H3B/C."
Saito 2022 confirms the H3 K27M oncohistone in H3F3A or HIST1H3B/C drives the midline paediatric-type subtype, supporting this mechanism's role in astrocytic-lineage diffuse midline glioma.
H3 G34R/V Hemispheric Oncohistone
In diffuse hemispheric glioma of adolescents and young adults, H3F3A glycine-to-arginine (G34R) or glycine-to-valine (G34V) substitutions alter local chromatin near the H3 N-terminal tail, perturbing K36 methylation and reprogramming gene expression in cis. G34-mutant tumours typically arise in cerebral hemispheres and co-occur with ATRX loss, TP53 mutation, and alternative lengthening of telomeres.
astrocyte link
Show evidence (2 references)
PMID:22286061 SUPPORT Human Clinical
"Mutations in ATRX (α-thalassaemia/mental retardation syndrome X-linked) and DAXX (death-domain associated protein), encoding two subunits of a chromatin remodelling complex required for H3.3 incorporation at pericentric heterochromatin and telomeres, were identified in 31% of samples overall,..."
Schwartzentruber et al. demonstrate near-universal co-occurrence of ATRX mutation with H3.3 G34R/G34V hemispheric mutations, supporting the H3 G34 oncohistone mechanism with ATRX cooperation.
PMID:23079654 SUPPORT Human Clinical
"the two H3F3A mutations give rise to GBMs in separate anatomic compartments"
Sturm et al. show that H3.3 K27M and G34 mutations define anatomically distinct subgroups (midline vs hemispheric), supporting separate hemispheric origin of G34-mutant tumours.
MGMT Promoter Methylation as a Therapy-Response Axis
Methylation of the O6-methylguanine-DNA methyltransferase (MGMT) promoter epigenetically silences MGMT expression and reduces tumour-cell capacity to repair O6-methylguanine adducts generated by alkylating chemotherapy (temozolomide). MGMT promoter methylation is a predictive biomarker for temozolomide benefit and is enriched in IDH-mutant astrocytomas through the G-CIMP background. It is a therapy-response axis, not an oncogenic driver per se.
DNA repair link ↓ DECREASED
Show evidence (1 reference)
PMID:15758010 SUPPORT Human Clinical
"Patients with glioblastoma containing a methylated MGMT promoter benefited from temozolomide, whereas those who did not have a methylated MGMT promoter did not have such a benefit."
Hegi et al. establish in a randomized clinical trial that MGMT promoter methylation predicts temozolomide benefit, directly supporting MGMT as a therapy-response axis in diffuse astrocytic glioma.
Chromosome 1p/19q Status Defines Astrocytic vs Oligodendroglial Lineage
Whole-arm codeletion of chromosomes 1p and 19q, in the context of IDH mutation, defines oligodendroglioma (curated separately). Its absence in IDH-mutant diffuse glioma, together with ATRX loss and TP53 mutation, operationally classifies the tumour as astrocytic. 1p/19q status is therefore used here purely as a lineage discriminator, not as a driver of astrocytic pathophysiology.
Show evidence (1 reference)
PMID:36651583 SUPPORT Human Clinical
"diffuse gliomas typically occurring in adults are classified as oligodendroglioma IDH-mutant and 1p/19q codeleted, astrocytoma IDH-mutant, and"
WHO CNS5 review confirms 1p/19q codeletion (in the context of IDH mutation) is the operational lineage discriminator separating oligodendroglioma from astrocytoma.

Histopathology

1
Diffuse Glioma VERY_FREQUENT
All molecularly defined subtypes of diffuse astrocytoma share the histologic pattern of diffuse parenchymal infiltration by atypical astrocytic cells, distinguishing them from circumscribed gliomas (e.g., pilocytic astrocytoma).
Show evidence (1 reference)
PMID:36651583 SUPPORT Human Clinical
"oligodendroglioma IDH-mutant and 1p/19q codeleted, astrocytoma IDH-mutant, and"
WHO CNS5 review lists astrocytoma IDH-mutant among diffuse gliomas, supporting the histopathologic class assignment.

Pathograph

Use the checkboxes to hide or show graph categories. Hover nodes for evidence and cross-linked metadata.
Pathograph: causal mechanism network for Diffuse Astrocytoma Interactive directed graph showing how pathophysiology mechanisms, phenotypes, genetic factors and variants, experimental models, environmental triggers, and treatments relate through causal and linked edges.

Phenotypes

4
Seizures VERY_FREQUENT Neurological HP:0001250
Headache FREQUENT Neurological HP:0002315
Cognitive Impairment FREQUENT Neurological HP:0100543
Hemiparesis FREQUENT Neurological HP:0001269
🧬

Genetic Associations

9
IDH1 (Somatic Mutation)
IDH2 (Somatic Mutation)
ATRX (Somatic Mutation)
Show evidence (1 reference)
PMID:26061751 SUPPORT Human Clinical
"Nearly all lower-grade gliomas with IDH mutations and no 1p/19q codeletion had mutations in TP53 (94%) and ATRX inactivation (86%)."
TCGA cohort supports ATRX inactivation at 86% in IDH-mutant non-codeleted (astrocytic-lineage) lower-grade gliomas.
TP53 (Somatic Mutation)
Show evidence (1 reference)
PMID:26061751 SUPPORT Human Clinical
"Nearly all lower-grade gliomas with IDH mutations and no 1p/19q codeletion had mutations in TP53 (94%) and ATRX inactivation (86%)."
TCGA cohort supports TP53 mutation at 94% in IDH-mutant non-codeleted (astrocytic-lineage) lower-grade gliomas.
H3F3A (Somatic Mutation)
Show evidence (1 reference)
PMID:22286061 SUPPORT Human Clinical
"Recurrent mutations in H3F3A, which encodes the replication-independent histone 3 variant H3.3, were observed in 31% of tumours, and led to amino acid substitutions at two critical positions within the histone tail (K27M, G34R/G34V)"
Schwartzentruber et al. establish that H3F3A K27M and G34R/G34V mutations are recurrent drivers in paediatric high-grade glioma, supporting the H3F3A assignment for paediatric-type subtypes.
HIST1H3B (Somatic Mutation)
CDKN2A (Homozygous Deletion)
MGMT (Epigenetic Silencing)
Show evidence (1 reference)
PMID:15758010 SUPPORT Human Clinical
"Epigenetic silencing of the MGMT (O6-methylguanine-DNA methyltransferase) DNA-repair gene by promoter methylation compromises DNA repair and has been associated with longer survival in patients with glioblastoma who receive alkylating agents."
Hegi et al. directly establish MGMT promoter methylation as an epigenetic silencing event predictive of temozolomide benefit in diffuse astrocytic glioma.
Chromosome 1p/19q (Co-deletion (Absence))
💊

Treatments

4
Maximal Safe Resection
Action: surgical procedure MAXO:0000004
Surgical resection maximising extent of resection while preserving neurological function. Greater extent of resection is associated with improved progression-free and overall survival across diffuse astrocytoma subtypes; however, complete resection is rarely achievable because of the infiltrative growth pattern.
Radiation Therapy
Action: radiation therapy MAXO:0000014
External beam radiation therapy is standard adjuvant treatment for higher grade or high-risk diffuse astrocytomas. In paediatric DMG H3 K27-altered, radiation provides only transient palliative benefit.
Temozolomide Chemotherapy
Action: chemotherapy MAXO:0000647
Oral alkylating chemotherapy used adjuvantly and at recurrence. MGMT promoter methylation predicts response and is enriched in IDH-mutant astrocytomas via the G-CIMP background.
Vorasidenib (Mutant-IDH Inhibitor)
Action: pharmacotherapy MAXO:0000058
Agent: vorasidenib
Brain-penetrant dual mutant IDH1/IDH2 inhibitor approved for residual or recurrent grade 2 IDH-mutant gliomas after the INDIGO trial demonstrated significant progression-free survival improvement. Targets the IDH-mutant subtype specifically.
🔬

Biochemical Markers

1
2-Hydroxyglutarate (2-HG)
🔬

Clinical Trials

1
NCT04164901 PHASE_III COMPLETED
INDIGO — pivotal Phase 3, multicentre, randomised, double-blind, placebo-controlled trial of vorasidenib (AG-881) in residual or recurrent Grade 2 IDH-mutant glioma after surgery. Demonstrated significant progression-free survival benefit and supported FDA approval of vorasidenib for the IDH-mutant subtype.
Target Phenotypes: Glioma
Show evidence (1 reference)
"Study AG881-C-004 is a phase 3, multicenter, randomized, double-blind, placebo-controlled study comparing the efficacy of vorasidenib to placebo in participants with residual or recurrent Grade 2 glioma with an IDH1 or IDH2 mutation who have undergone surgery as their only treatment."
Trial summary confirms the INDIGO study design (Phase 3 vorasidenib vs placebo) and the IDH-mutant Grade 2 glioma target population.
{ }

Source YAML

click to show
name: Diffuse Astrocytoma
creation_date: '2026-05-14T10:00:00Z'
updated_date: '2026-05-14T10:00:00Z'
description: >-
  Diffuse astrocytoma is an infiltrative glial neoplasm of the central nervous
  system characterised by diffuse parenchymal invasion of astrocytic tumour
  cells, with a strong tendency toward malignant progression to higher-grade
  glioma. Under the WHO 2021/CNS5 classification, "diffuse astrocytoma" is no
  longer a single morphologic grade-2 entity; it is now an umbrella for
  molecularly defined diffuse gliomas of astrocytic lineage, including
  IDH-mutant astrocytoma (adult-type, grades 2-4), diffuse midline glioma H3
  K27-altered (paediatric-type, grade 4), diffuse hemispheric glioma H3
  G34-mutant, and diffuse paediatric-type high-grade glioma H3-wildtype and
  IDH-wildtype. The defining biological feature across subtypes is diffuse
  infiltration of brain parenchyma along white-matter tracts, contrasted with
  the circumscribed growth of pilocytic astrocytoma. Common driver mechanisms
  include IDH1/2 neomorphic mutations producing the oncometabolite
  D-2-hydroxyglutarate (2-HG) with downstream global hypermethylation
  (G-CIMP), cooperating loss of ATRX and TP53 (adult astrocytic lineage),
  histone H3 oncomutations (K27M, G34R/V) acting as dominant-negative
  epigenetic drivers in paediatric tumours, and MGMT promoter methylation as a
  predictor of temozolomide response. Chromosome 1p/19q codeletion status is
  used here only to distinguish astrocytic from oligodendroglial lineage; the
  codeleted oligodendroglial entity is curated separately.
categories:
- Central Nervous System Neoplasm
- Adult Brain Tumor
- Pediatric Brain Tumor
- Molecularly Defined Tumor
parents:
- diffuse glioma
has_subtypes:
- name: IDH-mutant
  display_name: Astrocytoma, IDH-mutant (adult-type, grade 2-4)
  description: >-
    Adult-type diffuse astrocytic glioma defined by IDH1 or IDH2 mutation and
    absence of 1p/19q codeletion. WHO CNS5 graded 2-4 based on histology
    (mitoses, microvascular proliferation, necrosis) and CDKN2A/B homozygous
    deletion. Curated in detail under IDH_Mutant_Astrocytoma.yaml.
  subtype_term:
    preferred_term: astrocytoma, IDH-mutant, grade 2
    term:
      id: MONDO:0956994
      label: astrocytoma, IDH-mutant, grade 2
  evidence:
  - reference: PMID:26061751
    reference_title: "Comprehensive, Integrative Genomic Analysis of Diffuse Lower-Grade Gliomas."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "three robust, nonoverlapping, prognostically significant subtypes of lower-grade glioma that were captured more accurately by IDH, 1p/19q, and TP53 status than by histologic class"
    explanation: TCGA defines IDH-mutant astrocytic glioma (IDH-mutant, 1p/19q-intact, TP53-mutant) as one of three molecular subtypes that supersede histology, supporting the IDH-mutant subtype assignment.
- name: DMG H3K27-altered
  display_name: Diffuse midline glioma, H3 K27-altered (paediatric-type, grade 4)
  description: >-
    Highly aggressive paediatric-predominant midline glioma defined by H3 K27M
    mutation (H3F3A/HIST1H3B/C), EZHIP overexpression, or other mechanisms
    causing global H3K27me3 loss. Universally WHO grade 4. Curated in detail
    under H3_K27_Altered_Diffuse_Midline_Glioma.yaml.
  subtype_term:
    preferred_term: diffuse midline glioma, H3 K27-altered
    term:
      id: MONDO:1060171
      label: diffuse midline glioma, H3 K27-altered
  evidence:
  - reference: PMID:35169084
    reference_title: "[Diffuse midline glioma]."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Diffuse midline glioma(DMG), H3 K27M-mutant is an infiltrative midline high-grade glioma with predominantly astrocytic differentiation and a K27M mutation in either H3F3A or HIST1H3B/C."
    explanation: Saito 2022 review supports the DMG H3 K27-altered subtype definition with astrocytic differentiation, midline location, and K27M mutation in H3F3A or HIST1H3B/C.
- name: DHG H3G34-mutant
  display_name: Diffuse hemispheric glioma, H3 G34-mutant
  description: >-
    Hemispheric high-grade glioma of adolescents and young adults driven by
    glycine-to-arginine or glycine-to-valine substitution at H3F3A position
    G34. G34R/V mutations alter local chromatin and frequently co-occur with
    ATRX and TP53 loss. WHO grade 4.
  evidence:
  - reference: PMID:23079654
    reference_title: "Hotspot mutations in H3F3A and IDH1 define distinct epigenetic and biological subgroups of glioblastoma."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "the two H3F3A mutations give rise to GBMs in separate anatomic compartments"
    explanation: Sturm et al. show H3F3A K27M and G34 mutations occupy separate anatomic compartments (midline vs hemispheric), supporting DHG H3 G34-mutant as a distinct hemispheric subtype.
- name: Paediatric HGG
  display_name: Diffuse paediatric-type high-grade glioma, H3-wildtype and IDH-wildtype
  description: >-
    Paediatric high-grade diffuse glioma lacking IDH and histone H3 driver
    mutations. Heterogeneous molecular subgroups defined by methylation
    profiling (RTK1, RTK2, MYCN). Aggressive course with poor prognosis.
  evidence:
  - reference: PMID:34185076
    reference_title: "The 2021 WHO Classification of Tumors of the Central Nervous System: a summary."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "New tumor types and subtypes are introduced, some based on novel diagnostic technologies such as DNA methylome profiling."
    explanation: WHO CNS5 introduces methylation-profiled subtypes including diffuse paediatric-type high-grade glioma H3-wildtype/IDH-wildtype, supporting recognition of this subtype.
pathophysiology:
- name: Diffuse Parenchymal Infiltration
  description: >-
    The defining biological behaviour of all diffuse astrocytomas is single-cell
    infiltration of brain parenchyma along white-matter tracts and perineuronal
    spaces, in contrast with the circumscribed growth of pilocytic and other
    non-diffuse gliomas. This invasive pattern precludes complete surgical
    resection and underlies recurrence at the resection margin or at distant
    sites.
  evidence:
  - reference: PMID:34185076
    reference_title: "The 2021 WHO Classification of Tumors of the Central Nervous System: a summary."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "the 2021 fifth edition introduces major changes that advance the role of molecular diagnostics in CNS tumor classification."
    explanation: WHO CNS5 reframes diffuse astrocytoma as a molecularly defined umbrella entity rather than a single morphologic grade-2 lesion, supporting the diffuse-infiltration descriptor across subtypes.
  cell_types:
  - preferred_term: astrocyte
    term:
      id: CL:0000127
      label: astrocyte
  locations:
  - preferred_term: brain
    term:
      id: UBERON:0000955
      label: brain
- name: IDH1/2 Neomorphic Mutation and 2-HG Accumulation
  description: >-
    In the adult-type IDH-mutant subtype, heterozygous mutations in IDH1
    (R132H most common) or IDH2 (R172) confer neomorphic activity, converting
    alpha-ketoglutarate to the oncometabolite D-2-hydroxyglutarate (2-HG).
    2-HG accumulates to millimolar concentrations and competitively inhibits
    alpha-ketoglutarate-dependent dioxygenases.
  evidence:
  - reference: PMID:40916936
    reference_title: "Isocitrate dehydrogenase mutation and microenvironment in gliomas: do immunotherapy approaches matter?"
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "This unique immune microenvironment is shaped by 2-hydroxyglutarate (2-HG), an oncometabolite produced by mutant IDH."
    explanation: Confirms 2-HG as the oncometabolite produced by mutant IDH in gliomas, supporting the neomorphic enzyme activity described in this mechanism.
  cell_types:
  - preferred_term: astrocyte
    term:
      id: CL:0000127
      label: astrocyte
  biological_processes:
  - preferred_term: tricarboxylic acid cycle
    modifier: ABNORMAL
    term:
      id: GO:0006099
      label: tricarboxylic acid cycle
  downstream:
  - target: G-CIMP Hypermethylation and Blocked Differentiation
    description: 2-HG inhibits TET and Jumonji demethylases, causing genome-wide hypermethylation
- name: G-CIMP Hypermethylation and Blocked Differentiation
  description: >-
    Inhibition of TET2 (DNA demethylase) and Jumonji-domain histone demethylases
    by 2-HG produces the glioma CpG island methylator phenotype (G-CIMP) with
    global DNA and histone hypermethylation. The resulting transcriptional
    reprogramming blocks normal glial differentiation, sustaining cells in a
    proliferative progenitor-like state.
  evidence:
  - reference: PMID:22343889
    reference_title: "IDH1 mutation is sufficient to establish the glioma hypermethylator phenotype."
    supports: SUPPORT
    evidence_source: IN_VITRO
    snippet: "mutation of a single gene, isocitrate dehydrogenase 1 (IDH1), establishes G-CIMP by remodelling the methylome."
    explanation: Turcan et al. demonstrate in primary human astrocytes that mutant IDH1 is sufficient to establish the glioma CpG island methylator phenotype, directly supporting the G-CIMP mechanism downstream of 2-HG.
  biological_processes:
  - preferred_term: DNA methylation
    modifier: INCREASED
    term:
      id: GO:0006304
      label: DNA modification
  - preferred_term: glial cell differentiation
    modifier: DECREASED
    term:
      id: GO:0010001
      label: glial cell differentiation
  downstream:
  - target: ATRX/TP53 Cooperative Loss
    description: G-CIMP background cooperates with ATRX/TP53 loss to define astrocytic lineage
- name: ATRX/TP53 Cooperative Loss
  description: >-
    In the adult IDH-mutant astrocytic lineage, ATRX loss-of-function mutations
    co-occur with TP53 mutations at very high frequency (approximately 86% ATRX
    and 94% TP53 in IDH-mutant non-codeleted lower-grade gliomas), and are
    mutually exclusive with 1p/19q codeletion. ATRX loss licenses alternative
    lengthening of telomeres (ALT); TP53 loss removes a key tumour-suppressor
    checkpoint. The ATRX-mutant / TP53-mutant / 1p19q-intact triad operationally
    defines astrocytic versus oligodendroglial differentiation in IDH-mutant
    diffuse glioma.
  evidence:
  - reference: PMID:26061751
    reference_title: "Comprehensive, Integrative Genomic Analysis of Diffuse Lower-Grade Gliomas."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Nearly all lower-grade gliomas with IDH mutations and no 1p/19q codeletion had mutations in TP53 (94%) and ATRX inactivation (86%)."
    explanation: TCGA cohort directly quantifies the ATRX/TP53 cooperative loss in IDH-mutant non-codeleted (astrocytic-lineage) diffuse glioma.
  cell_types:
  - preferred_term: astrocyte
    term:
      id: CL:0000127
      label: astrocyte
- name: H3 K27M Oncohistone Dominant-Negative PRC2 Inhibition
  description: >-
    In paediatric-type midline diffuse astrocytomas, the H3 K27M substitution
    in H3F3A or HIST1H3B/C generates an oncohistone that, although present in
    only a minority of total H3 molecules, sequesters and inhibits PRC2/EZH2.
    Global loss of H3K27 trimethylation derepresses developmental and
    proliferative programmes, driving aggressive growth in midline structures
    (pons, thalamus, spinal cord).
  evidence:
  - reference: PMID:35169084
    reference_title: "[Diffuse midline glioma]."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Diffuse midline glioma(DMG), H3 K27M-mutant is an infiltrative midline high-grade glioma with predominantly astrocytic differentiation and a K27M mutation in either H3F3A or HIST1H3B/C."
    explanation: Saito 2022 confirms the H3 K27M oncohistone in H3F3A or HIST1H3B/C drives the midline paediatric-type subtype, supporting this mechanism's role in astrocytic-lineage diffuse midline glioma.
  cell_types:
  - preferred_term: astrocyte
    term:
      id: CL:0000127
      label: astrocyte
  molecular_functions:
  - preferred_term: histone H3K27 methyltransferase activity
    modifier: DECREASED
    term:
      id: GO:0046976
      label: histone H3K27 methyltransferase activity
  locations:
  - preferred_term: pons
    term:
      id: UBERON:0000988
      label: pons
- name: H3 G34R/V Hemispheric Oncohistone
  description: >-
    In diffuse hemispheric glioma of adolescents and young adults, H3F3A
    glycine-to-arginine (G34R) or glycine-to-valine (G34V) substitutions alter
    local chromatin near the H3 N-terminal tail, perturbing K36 methylation and
    reprogramming gene expression in cis. G34-mutant tumours typically arise in
    cerebral hemispheres and co-occur with ATRX loss, TP53 mutation, and
    alternative lengthening of telomeres.
  evidence:
  - reference: PMID:22286061
    reference_title: "Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastoma."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Mutations in ATRX (α-thalassaemia/mental retardation syndrome X-linked) and DAXX (death-domain associated protein), encoding two subunits of a chromatin remodelling complex required for H3.3 incorporation at pericentric heterochromatin and telomeres, were identified in 31% of samples overall, and in 100% of tumours harbouring a G34R or G34V H3.3 mutation."
    explanation: Schwartzentruber et al. demonstrate near-universal co-occurrence of ATRX mutation with H3.3 G34R/G34V hemispheric mutations, supporting the H3 G34 oncohistone mechanism with ATRX cooperation.
  - reference: PMID:23079654
    reference_title: "Hotspot mutations in H3F3A and IDH1 define distinct epigenetic and biological subgroups of glioblastoma."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "the two H3F3A mutations give rise to GBMs in separate anatomic compartments"
    explanation: Sturm et al. show that H3.3 K27M and G34 mutations define anatomically distinct subgroups (midline vs hemispheric), supporting separate hemispheric origin of G34-mutant tumours.
  cell_types:
  - preferred_term: astrocyte
    term:
      id: CL:0000127
      label: astrocyte
- name: MGMT Promoter Methylation as a Therapy-Response Axis
  description: >-
    Methylation of the O6-methylguanine-DNA methyltransferase (MGMT) promoter
    epigenetically silences MGMT expression and reduces tumour-cell capacity to
    repair O6-methylguanine adducts generated by alkylating chemotherapy
    (temozolomide). MGMT promoter methylation is a predictive biomarker for
    temozolomide benefit and is enriched in IDH-mutant astrocytomas through the
    G-CIMP background. It is a therapy-response axis, not an oncogenic driver
    per se.
  evidence:
  - reference: PMID:15758010
    reference_title: "MGMT gene silencing and benefit from temozolomide in glioblastoma."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Patients with glioblastoma containing a methylated MGMT promoter benefited from temozolomide, whereas those who did not have a methylated MGMT promoter did not have such a benefit."
    explanation: Hegi et al. establish in a randomized clinical trial that MGMT promoter methylation predicts temozolomide benefit, directly supporting MGMT as a therapy-response axis in diffuse astrocytic glioma.
  biological_processes:
  - preferred_term: DNA repair
    modifier: DECREASED
    term:
      id: GO:0006281
      label: DNA repair
- name: Chromosome 1p/19q Status Defines Astrocytic vs Oligodendroglial Lineage
  description: >-
    Whole-arm codeletion of chromosomes 1p and 19q, in the context of IDH
    mutation, defines oligodendroglioma (curated separately). Its absence in
    IDH-mutant diffuse glioma, together with ATRX loss and TP53 mutation,
    operationally classifies the tumour as astrocytic. 1p/19q status is
    therefore used here purely as a lineage discriminator, not as a driver of
    astrocytic pathophysiology.
  evidence:
  - reference: PMID:36651583
    reference_title: "IDH-mutant diffuse gliomas: tips and tricks in the era of genomic tumor classification."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "diffuse gliomas typically occurring in adults are classified as oligodendroglioma IDH-mutant and 1p/19q codeleted, astrocytoma IDH-mutant, and"
    explanation: WHO CNS5 review confirms 1p/19q codeletion (in the context of IDH mutation) is the operational lineage discriminator separating oligodendroglioma from astrocytoma.
phenotypes:
- category: Neurological
  name: Seizures
  frequency: VERY_FREQUENT
  description: >-
    Seizures are the most common presenting symptom of supratentorial diffuse
    astrocytoma, occurring in approximately 60-80% of patients. Lower-grade
    tumours have higher seizure frequency than higher-grade lesions.
  phenotype_term:
    preferred_term: Seizure
    term:
      id: HP:0001250
      label: Seizure
- category: Neurological
  name: Headache
  frequency: FREQUENT
  description: >-
    Headache from mass effect or raised intracranial pressure, particularly
    in higher-grade tumours and those with peritumoural oedema.
  phenotype_term:
    preferred_term: Headache
    term:
      id: HP:0002315
      label: Headache
- category: Neurological
  name: Cognitive Impairment
  frequency: FREQUENT
  description: >-
    Memory loss, executive dysfunction, and personality change, especially
    with frontal lobe involvement.
  phenotype_term:
    preferred_term: Cognitive impairment
    term:
      id: HP:0100543
      label: Cognitive impairment
- category: Neurological
  name: Hemiparesis
  frequency: FREQUENT
  description: >-
    Unilateral motor weakness is the most common focal neurological deficit
    seen with diffuse astrocytomas affecting corticospinal pathways; other
    focal deficits (sensory loss, aphasia, visual field defects) co-occur
    depending on tumour location but are not enumerated separately here.
  phenotype_term:
    preferred_term: Hemiparesis
    term:
      id: HP:0001269
      label: Hemiparesis
genetic:
- name: IDH1
  association: Somatic Mutation
  notes: >-
    IDH1 R132H is the dominant driver in the adult-type IDH-mutant subtype
    (>90% of IDH-mutant astrocytomas). Detectable by R132H-specific
    immunohistochemistry; other R132 variants require sequencing.
- name: IDH2
  association: Somatic Mutation
  notes: >-
    IDH2 R172 mutations account for ~3% of IDH-mutant diffuse gliomas and are
    enriched in oligodendrogliomas; in astrocytic lineage they require
    sequencing for detection.
- name: ATRX
  association: Somatic Mutation
  evidence:
  - reference: PMID:26061751
    reference_title: "Comprehensive, Integrative Genomic Analysis of Diffuse Lower-Grade Gliomas."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Nearly all lower-grade gliomas with IDH mutations and no 1p/19q codeletion had mutations in TP53 (94%) and ATRX inactivation (86%)."
    explanation: TCGA cohort supports ATRX inactivation at 86% in IDH-mutant non-codeleted (astrocytic-lineage) lower-grade gliomas.
  notes: >-
    Inactivating ATRX mutations occur in ~70-86% of IDH-mutant astrocytomas
    and are associated with alternative lengthening of telomeres (ALT). ATRX
    loss is mutually exclusive with 1p/19q codeletion and is part of the
    operational definition of astrocytic versus oligodendroglial lineage.
- name: TP53
  association: Somatic Mutation
  evidence:
  - reference: PMID:26061751
    reference_title: "Comprehensive, Integrative Genomic Analysis of Diffuse Lower-Grade Gliomas."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Nearly all lower-grade gliomas with IDH mutations and no 1p/19q codeletion had mutations in TP53 (94%) and ATRX inactivation (86%)."
    explanation: TCGA cohort supports TP53 mutation at 94% in IDH-mutant non-codeleted (astrocytic-lineage) lower-grade gliomas.
  notes: >-
    TP53 mutations occur in up to 94% of IDH-mutant non-1p19q-codeleted
    diffuse gliomas; in the WHO CNS5 framework they support an astrocytic
    rather than oligodendroglial diagnosis.
- name: H3F3A
  association: Somatic Mutation
  evidence:
  - reference: PMID:22286061
    reference_title: "Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastoma."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Recurrent mutations in H3F3A, which encodes the replication-independent histone 3 variant H3.3, were observed in 31% of tumours, and led to amino acid substitutions at two critical positions within the histone tail (K27M, G34R/G34V)"
    explanation: Schwartzentruber et al. establish that H3F3A K27M and G34R/G34V mutations are recurrent drivers in paediatric high-grade glioma, supporting the H3F3A assignment for paediatric-type subtypes.
  notes: >-
    K27M mutations drive the midline paediatric-type subtype (DMG, H3
    K27-altered). G34R or G34V substitutions drive the hemispheric
    paediatric/young-adult subtype (DHG, H3 G34-mutant). H3F3A encodes the
    replication-independent histone variant H3.3.
- name: HIST1H3B
  association: Somatic Mutation
  notes: >-
    H3.1 K27M mutations in HIST1H3B (and less commonly HIST1H3C) drive a
    subset of pontine paediatric midline diffuse astrocytomas (DIPG variant).
- name: CDKN2A
  association: Homozygous Deletion
  notes: >-
    Homozygous deletion of CDKN2A/CDKN2B (9p21) upgrades IDH-mutant
    astrocytoma to WHO grade 4 independent of histology and predicts poor
    outcome.
- name: MGMT
  association: Epigenetic Silencing
  evidence:
  - reference: PMID:15758010
    reference_title: "MGMT gene silencing and benefit from temozolomide in glioblastoma."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "Epigenetic silencing of the MGMT (O6-methylguanine-DNA methyltransferase) DNA-repair gene by promoter methylation compromises DNA repair and has been associated with longer survival in patients with glioblastoma who receive alkylating agents."
    explanation: Hegi et al. directly establish MGMT promoter methylation as an epigenetic silencing event predictive of temozolomide benefit in diffuse astrocytic glioma.
  notes: >-
    Promoter methylation of MGMT silences expression and predicts response to
    temozolomide; enriched in IDH-mutant tumours via the G-CIMP background.
- name: Chromosome 1p/19q
  association: Co-deletion (Absence)
  notes: >-
    Whole-arm codeletion of 1p and 19q is required for the oligodendroglioma
    diagnosis. Its absence, in the presence of IDH mutation and ATRX/TP53
    alterations, defines astrocytic lineage in IDH-mutant diffuse glioma.
biochemical:
- name: 2-Hydroxyglutarate (2-HG)
  notes: >-
    D-2-hydroxyglutarate accumulates to millimolar intratumoural concentrations
    in IDH-mutant astrocytomas. Detectable non-invasively by MR spectroscopy
    (peak at 2.25 ppm). Serves as a pharmacodynamic biomarker for IDH-inhibitor
    therapy.
histopathology:
- name: Diffuse Glioma
  finding_term:
    preferred_term: Diffuse Glioma
    term:
      id: NCIT:C129325
      label: Diffuse Glioma
  frequency: VERY_FREQUENT
  description: >-
    All molecularly defined subtypes of diffuse astrocytoma share the histologic
    pattern of diffuse parenchymal infiltration by atypical astrocytic cells,
    distinguishing them from circumscribed gliomas (e.g., pilocytic astrocytoma).
  evidence:
  - reference: PMID:36651583
    reference_title: "IDH-mutant diffuse gliomas: tips and tricks in the era of genomic tumor classification."
    supports: SUPPORT
    evidence_source: HUMAN_CLINICAL
    snippet: "oligodendroglioma IDH-mutant and 1p/19q codeleted, astrocytoma IDH-mutant, and"
    explanation: WHO CNS5 review lists astrocytoma IDH-mutant among diffuse gliomas, supporting the histopathologic class assignment.
treatments:
- name: Maximal Safe Resection
  description: >-
    Surgical resection maximising extent of resection while preserving
    neurological function. Greater extent of resection is associated with
    improved progression-free and overall survival across diffuse astrocytoma
    subtypes; however, complete resection is rarely achievable because of the
    infiltrative growth pattern.
  treatment_term:
    preferred_term: surgical procedure
    term:
      id: MAXO:0000004
      label: surgical procedure
- name: Radiation Therapy
  description: >-
    External beam radiation therapy is standard adjuvant treatment for higher
    grade or high-risk diffuse astrocytomas. In paediatric DMG H3 K27-altered,
    radiation provides only transient palliative benefit.
  treatment_term:
    preferred_term: radiation therapy
    term:
      id: MAXO:0000014
      label: radiation therapy
- name: Temozolomide Chemotherapy
  description: >-
    Oral alkylating chemotherapy used adjuvantly and at recurrence. MGMT
    promoter methylation predicts response and is enriched in IDH-mutant
    astrocytomas via the G-CIMP background.
  treatment_term:
    preferred_term: chemotherapy
    term:
      id: MAXO:0000647
      label: chemotherapy
- name: Vorasidenib (Mutant-IDH Inhibitor)
  description: >-
    Brain-penetrant dual mutant IDH1/IDH2 inhibitor approved for residual or
    recurrent grade 2 IDH-mutant gliomas after the INDIGO trial demonstrated
    significant progression-free survival improvement. Targets the IDH-mutant
    subtype specifically.
  treatment_term:
    preferred_term: pharmacotherapy
    term:
      id: MAXO:0000058
      label: pharmacotherapy
    therapeutic_agent:
    - preferred_term: vorasidenib
      term:
        id: NCIT:C152914
        label: Vorasidenib
clinical_trials:
- name: NCT04164901
  phase: PHASE_III
  status: COMPLETED
  description: >-
    INDIGO — pivotal Phase 3, multicentre, randomised, double-blind,
    placebo-controlled trial of vorasidenib (AG-881) in residual or recurrent
    Grade 2 IDH-mutant glioma after surgery. Demonstrated significant
    progression-free survival benefit and supported FDA approval of
    vorasidenib for the IDH-mutant subtype.
  target_phenotypes:
  - preferred_term: Glioma
    term:
      id: HP:0009733
      label: Glioma
  evidence:
  - reference: clinicaltrials:NCT04164901
    supports: SUPPORT
    snippet: "Study AG881-C-004 is a phase 3, multicenter, randomized, double-blind, placebo-controlled study comparing the efficacy of vorasidenib to placebo in participants with residual or recurrent Grade 2 glioma with an IDH1 or IDH2 mutation who have undergone surgery as their only treatment."
    explanation: Trial summary confirms the INDIGO study design (Phase 3 vorasidenib vs placebo) and the IDH-mutant Grade 2 glioma target population.
disease_term:
  preferred_term: diffuse astrocytoma
  term:
    id: MONDO:0016686
    label: diffuse astrocytoma
classifications:
  icdo_morphology:
    classification_value: Glioma
  harrisons_chapter:
  - classification_value: cancer
  - classification_value: solid tumor